##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431391_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4619639
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60946731984902	34.0	31.0	34.0	31.0	34.0
2	32.707068236284265	34.0	31.0	34.0	31.0	34.0
3	32.80622966426598	34.0	31.0	34.0	31.0	34.0
4	36.28947846357691	37.0	37.0	37.0	35.0	37.0
5	36.19980262527007	37.0	37.0	37.0	35.0	37.0
6	36.24697341069292	37.0	37.0	37.0	35.0	37.0
7	36.19488384265524	37.0	37.0	37.0	35.0	37.0
8	36.20584876870249	37.0	37.0	37.0	35.0	37.0
9	37.99058714328111	39.0	39.0	39.0	35.0	39.0
10-11	37.96892668453098	39.0	38.5	39.0	35.0	39.0
12-13	37.95950484442615	39.0	38.0	39.0	35.0	39.0
14-15	39.41811221612771	41.0	39.0	41.0	36.0	41.0
16-17	39.44987995815258	41.0	39.0	41.0	36.0	41.0
18-19	39.51977687001084	41.0	39.5	41.0	36.5	41.0
20-21	39.516534625324624	41.0	40.0	41.0	36.0	41.0
22-23	39.465847764295006	41.0	39.0	41.0	36.0	41.0
24-25	39.42398745876031	41.0	39.0	41.0	36.0	41.0
26-27	39.3149851103084	41.0	39.0	41.0	36.0	41.0
28-29	39.235716362252546	41.0	39.0	41.0	35.5	41.0
30-31	39.06906210636805	41.0	39.0	41.0	35.0	41.0
32-33	38.866406552546636	41.0	38.0	41.0	35.0	41.0
34-35	38.744452650953896	41.0	38.0	41.0	35.0	41.0
36-37	38.610154494755974	41.0	38.0	41.0	35.0	41.0
38-39	38.46785300756184	41.0	38.0	41.0	35.0	41.0
40-41	38.33428304679219	40.0	38.0	41.0	34.5	41.0
42-43	38.166410297428	40.0	38.0	41.0	34.0	41.0
44-45	37.92982871605335	40.0	38.0	41.0	33.5	41.0
46-47	37.5746331044482	40.0	38.0	41.0	33.0	41.0
48-49	37.24771329534624	40.0	37.5	41.0	32.0	41.0
50-51	37.08139304824468	40.0	37.0	41.0	32.0	41.0
52-53	37.022218943947784	40.0	37.0	41.0	32.0	41.0
54-55	36.87937477365655	40.0	37.0	41.0	31.0	41.0
56-57	36.730557625823145	40.0	36.0	41.0	31.0	41.0
58-59	36.54895826275603	40.0	36.0	41.0	31.0	41.0
60-61	36.39430191406731	39.5	35.0	41.0	31.0	41.0
62-63	36.23185318160142	39.0	35.0	41.0	31.0	41.0
64-65	35.997672004241025	39.0	35.0	41.0	30.5	41.0
66-67	35.72793426066409	39.0	35.0	41.0	30.0	41.0
68-69	35.431725076353366	38.0	35.0	41.0	30.0	41.0
70-71	35.129056945791646	37.0	35.0	40.0	29.0	41.0
72-73	34.805281213532055	37.0	35.0	39.5	29.0	41.0
74-75	34.467104572456854	36.0	35.0	39.0	29.0	41.0
76-77	34.1298828761295	36.0	35.0	39.0	29.0	41.0
78-79	33.80426479211904	35.5	34.0	38.0	29.0	39.5
80-81	33.537389609880776	35.0	34.0	37.0	29.0	39.0
82-83	33.33278238840741	35.0	34.0	37.0	29.0	39.0
84-85	33.06597355334475	35.0	34.0	36.5	29.0	38.5
86-87	32.821897771665704	35.0	34.0	36.0	29.0	37.0
88-89	32.586880273545184	35.0	34.0	36.0	29.0	37.0
90-91	32.40309437598912	35.0	34.0	35.5	29.0	36.5
92-93	32.24254774020221	35.0	34.0	35.0	28.0	36.0
94-95	32.10277567143234	35.0	34.0	35.0	27.0	36.0
96-97	31.961101073049214	35.0	34.0	35.0	27.0	36.0
98-99	31.843781299794205	35.0	34.0	35.0	27.0	36.0
100	31.740969370117448	35.0	34.0	35.0	26.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.021310490745285904
1101	2	-0.007218900418124008
1101	3	0.004105028429954416
1101	4	0.020929833019124544
1101	5	0.015936668036466983
1101	6	0.03186768996937417
1101	7	0.018928008316478895
1101	8	0.051463058432226205
1101	9	0.018888442653050674
1101	10-11	0.009429349732904768
1101	12-13	0.07768289973434861
1101	14-15	0.06942449254987793
1101	16-17	0.013985336507893464
1101	18-19	-0.0035386452411430014
1101	20-21	0.02601837173617838
1101	22-23	0.018425217902532154
1101	24-25	0.019530244414561082
1101	26-27	0.039954932794742604
1101	28-29	-0.02463702054991046
1101	30-31	-0.038237824455052305
1101	32-33	-0.01777015560058004
1101	34-35	0.0214995872263799
1101	36-37	0.008689955424266316
1101	38-39	-0.06335349294478476
1101	40-41	-0.07858268093565357
1101	42-43	-0.1010509642841555
1101	44-45	-0.1091224686491401
1101	46-47	-0.10374891180548218
1101	48-49	-0.024321064665883796
1101	50-51	-0.2178267995080816
1101	52-53	-0.1478340158930891
1101	54-55	-0.16286007828239946
1101	56-57	-0.14576328595578758
1101	58-59	-0.11967418208430303
1101	60-61	-0.15211778652226116
1101	62-63	-0.174626098076331
1101	64-65	-0.1971499166407611
1101	66-67	-0.19909853939408606
1101	68-69	-0.14971206072169707
1101	70-71	-0.12734597582495866
1101	72-73	-0.19615700913262657
1101	74-75	-0.229819410779605
1101	76-77	-0.1702165194215297
1101	78-79	-0.1605832402970151
1101	80-81	-0.10633308355367177
1101	82-83	-0.09343108287884405
1101	84-85	-0.07067396195461839
1101	86-87	-0.08217613584013606
1101	88-89	-0.09314353429407873
1101	90-91	-0.10740013058339315
1101	92-93	-0.1036015591126116
1101	94-95	-0.05212433450070364
1101	96-97	-0.06123473317380146
1101	98-99	-0.01728225538486683
1101	100	0.03776812124359452
1102	1	-0.035233800352877154
1102	2	-0.01683776603884013
1102	3	0.011757058317122926
1102	4	-0.0025597146135893922
1102	5	-0.03643638178515829
1102	6	-0.062406532501896095
1102	7	-0.04385035968810058
1102	8	-0.015195170385887025
1102	9	0.03019994914568258
1102	10-11	-0.0033602945617658975
1102	12-13	-0.022239328376059575
1102	14-15	-0.06774340626805753
1102	16-17	-0.05129814984771741
1102	18-19	-0.04859562959633479
1102	20-21	0.028138997472375138
1102	22-23	0.020334791775681538
1102	24-25	0.007193130812375159
1102	26-27	0.028089155440696345
1102	28-29	0.011394705760650936
1102	30-31	0.02967164292371649
1102	32-33	0.06007170897519387
1102	34-35	0.05996282961970678
1102	36-37	0.05100682241588572
1102	38-39	0.025415596807157215
1102	40-41	0.08620409927994643
1102	42-43	0.058536150233649664
1102	44-45	0.09140856643399786
1102	46-47	0.049823805686052935
1102	48-49	0.012709292994657062
1102	50-51	-0.07524135114842068
1102	52-53	-0.06518440889398391
1102	54-55	-0.09918591080505479
1102	56-57	-0.09086874215885388
1102	58-59	-0.0429685745352586
1102	60-61	-0.07240096579089794
1102	62-63	-0.04800741163664668
1102	64-65	-0.10466711703304554
1102	66-67	-0.09921666927384365
1102	68-69	-0.09250756784203418
1102	70-71	-0.09096610022053397
1102	72-73	-0.03187125258723711
1102	74-75	-0.10307915733987016
1102	76-77	-0.055103733982072356
1102	78-79	-0.04265729868805579
1102	80-81	-0.07150325763254273
1102	82-83	-0.10336027538377834
1102	84-85	-0.06300298564624285
1102	86-87	-0.08365892220648874
1102	88-89	-0.09889792866644598
1102	90-91	-0.12322532259022978
1102	92-93	-0.13807284690297905
1102	94-95	-0.1756963096305988
1102	96-97	-0.20705096894672792
1102	98-99	-0.1715490528311463
1102	100	-0.1823400607085368
1103	1	-0.08026622454617183
1103	2	-0.11980236989163728
1103	3	-0.062174860190708614
1103	4	-0.04125794870148525
1103	5	-0.022306685379035684
1103	6	-0.04841947850488992
1103	7	-0.05479328978616138
1103	8	-0.04680684333405338
1103	9	-0.024707789340354225
1103	10-11	1.8960426718450663E-4
1103	12-13	0.00971782457872905
1103	14-15	-0.015310242885199443
1103	16-17	-0.010630760012951157
1103	18-19	-0.051504543723766005
1103	20-21	-0.05299722295432474
1103	22-23	-0.06430381824400655
1103	24-25	-0.05445576909760774
1103	26-27	-0.038418441311378615
1103	28-29	-0.034106754569108944
1103	30-31	-0.018367971382950543
1103	32-33	-0.010660768742262405
1103	34-35	-0.008025376069085155
1103	36-37	0.025224057858260096
1103	38-39	-0.04278950884595645
1103	40-41	-0.09848654149691782
1103	42-43	-0.061530746257510316
1103	44-45	-0.09911608693669649
1103	46-47	-0.09265359427821807
1103	48-49	-0.05809464356879346
1103	50-51	-0.1611837352687786
1103	52-53	-0.07626281257631717
1103	54-55	-0.06498124523749738
1103	56-57	-0.050217789005664315
1103	58-59	-0.12032703073422368
1103	60-61	-0.02381441029447018
1103	62-63	-0.14072154799031011
1103	64-65	-0.16808365415618454
1103	66-67	-0.1290469638054148
1103	68-69	-0.04326218013775929
1103	70-71	-0.10579556569185655
1103	72-73	-0.10211394472160151
1103	74-75	-0.08550520190761546
1103	76-77	-0.06853052998030051
1103	78-79	-0.09344472929038972
1103	80-81	-0.06373469464309522
1103	82-83	-0.08572267225707719
1103	84-85	-0.06883076996504656
1103	86-87	-0.035108761399996524
1103	88-89	-0.03562573153013915
1103	90-91	-0.04164544940203996
1103	92-93	-0.07111944100690692
1103	94-95	-0.05827344779167731
1103	96-97	-0.014713509031356153
1103	98-99	-0.00807340788695754
1103	100	0.05792754269069178
1104	1	-0.10224773448040736
1104	2	-0.10574023761321172
1104	3	-0.10285949815626338
1104	4	-0.04743727625282901
1104	5	-0.055651803574825465
1104	6	-0.09862453533846605
1104	7	-0.08951340943681174
1104	8	-0.13093381338011767
1104	9	-0.10051528024300893
1104	10-11	-0.12413155895523431
1104	12-13	-0.09463922730471097
1104	14-15	-0.13665611214192808
1104	16-17	-0.0993350975373346
1104	18-19	-0.08994434802955453
1104	20-21	-0.07125122090065616
1104	22-23	-0.088845943667053
1104	24-25	-0.09212895989529102
1104	26-27	-0.11494491243190907
1104	28-29	-0.11459671474432298
1104	30-31	-0.07920885334684158
1104	32-33	-0.06227783102993101
1104	34-35	-0.07817403436101955
1104	36-37	-0.0728576285040532
1104	38-39	-0.06739812122161482
1104	40-41	-0.13036462468612342
1104	42-43	-0.10117669716579769
1104	44-45	-0.007391193044128386
1104	46-47	-0.1294350249953098
1104	48-49	-0.1289527644957289
1104	50-51	-0.17035701760087818
1104	52-53	-0.12474471046265734
1104	54-55	-0.060281260889894384
1104	56-57	-0.1381102052436134
1104	58-59	-0.07623316962509108
1104	60-61	-0.06443025848341932
1104	62-63	-0.06518808179801994
1104	64-65	-0.05594163920515882
1104	66-67	-0.1097832059011381
1104	68-69	-0.09508986574044087
1104	70-71	-0.1310262922065064
1104	72-73	-0.13717752546497763
1104	74-75	-0.06501581982063698
1104	76-77	-0.12560229030909653
1104	78-79	-0.11720570959190013
1104	80-81	-0.09027221137799302
1104	82-83	-0.09303819955033532
1104	84-85	-0.07961346535223157
1104	86-87	-0.07080148953787813
1104	88-89	-0.06505299742443071
1104	90-91	-0.05228231817671514
1104	92-93	-0.06906666059878575
1104	94-95	-0.09290624163423189
1104	96-97	-0.06213853549170878
1104	98-99	-0.11477886378972713
1104	100	-0.09633450692656709
1105	1	-0.14628297224719233
1105	2	-0.14388077971442215
1105	3	-0.11762591889276308
1105	4	-0.04423770750167222
1105	5	-0.07739017269513226
1105	6	-0.04047105639623538
1105	7	-0.028694117337522584
1105	8	-0.01102556566358004
1105	9	-0.015836306122871235
1105	10-11	-0.06201974192871518
1105	12-13	-0.09303794937648036
1105	14-15	-0.13396836382347033
1105	16-17	-0.1176280524516855
1105	18-19	-0.1507519257718215
1105	20-21	-0.10263805164055384
1105	22-23	-0.10167775673097168
1105	24-25	-0.13570439521628685
1105	26-27	-0.04822276585701246
1105	28-29	-0.09347203972298956
1105	30-31	-0.0938780259719465
1105	32-33	-0.158066886378613
1105	34-35	-0.17494932488320103
1105	36-37	-0.1474626568321824
1105	38-39	-0.15121425659705778
1105	40-41	-0.1805685470095284
1105	42-43	-0.20517770627238718
1105	44-45	-0.1604719920359443
1105	46-47	-0.20946207301099662
1105	48-49	-0.18142386631382124
1105	50-51	-0.14094395013417937
1105	52-53	-0.12729203139366518
1105	54-55	-0.1284103006293833
1105	56-57	-0.06551768339043207
1105	58-59	-0.10504417932843779
1105	60-61	-0.09849441611091692
1105	62-63	-0.15119804539924786
1105	64-65	-0.1563146528665058
1105	66-67	-0.22720202457098537
1105	68-69	-0.09428003696912413
1105	70-71	-0.06936831456106773
1105	72-73	-0.04501954183418633
1105	74-75	-0.07594710942110083
1105	76-77	-0.15453947177416438
1105	78-79	-0.048771260626004675
1105	80-81	-0.02544201705764948
1105	82-83	-0.10191322381221823
1105	84-85	-0.12062687827988583
1105	86-87	-0.11273373828893796
1105	88-89	-0.04430178858860501
1105	90-91	-0.10450852194281879
1105	92-93	-0.11534446767917217
1105	94-95	-0.09114714869060236
1105	96-97	-0.09251095418409605
1105	98-99	-0.062035344654656654
1105	100	-0.058406517431009775
1106	1	-0.09963678719983449
1106	2	-0.048184288797891384
1106	3	-0.05637177096101453
1106	4	-0.007811686796621586
1106	5	8.806371922247536E-4
1106	6	-0.05227650437912956
1106	7	-0.005097582389900879
1106	8	-0.014415694556198844
1106	9	-0.08527534898886557
1106	10-11	-0.07662007079819233
1106	12-13	-0.06352957712964269
1106	14-15	-0.07675265297326206
1106	16-17	-0.07710118362377472
1106	18-19	-0.05682325459509485
1106	20-21	-0.05519125602749142
1106	22-23	-0.07134373391760107
1106	24-25	-0.030197881997857223
1106	26-27	-0.07905850649650858
1106	28-29	-0.05218835603588445
1106	30-31	-0.10061158345377663
1106	32-33	-0.08764540949970723
1106	34-35	-0.09341088666789688
1106	36-37	-0.08387271629133153
1106	38-39	-0.08684345458991061
1106	40-41	-0.18023679669231285
1106	42-43	-0.08815689581815178
1106	44-45	-0.0762145163863508
1106	46-47	-0.06883025861339576
1106	48-49	-0.044668749380470274
1106	50-51	-0.06964798442923836
1106	52-53	-0.06824884319061653
1106	54-55	-0.09996508643436641
1106	56-57	-0.11145655050132319
1106	58-59	-0.06941113517417818
1106	60-61	-0.06661288021449252
1106	62-63	-0.15000045526016237
1106	64-65	-0.12378998090725446
1106	66-67	-0.055271159333031505
1106	68-69	-0.11000888271966858
1106	70-71	-0.029936943070225652
1106	72-73	0.03909051202524694
1106	74-75	0.014608428534110374
1106	76-77	-0.010914583641636
1106	78-79	0.006042262201070514
1106	80-81	-0.02172642214297582
1106	82-83	-0.05269236735521332
1106	84-85	0.011507770021701447
1106	86-87	-0.03556658884371444
1106	88-89	-0.07832605318084518
1106	90-91	-0.11319162543183836
1106	92-93	-0.053279116581109065
1106	94-95	-0.13796440261818077
1106	96-97	-0.10584695197220029
1106	98-99	-0.11178588513103449
1106	100	-0.08220466811627247
1107	1	-0.2409665463796955
1107	2	-0.2095289456128384
1107	3	-0.1481694282531052
1107	4	-0.20260559628866304
1107	5	-0.28477816035015735
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2216	22-23	-0.23331680124743315
2216	24-25	-0.22449066760781733
2216	26-27	-0.18130519682955537
2216	28-29	-0.178498824415378
2216	30-31	-0.27398362940217424
2216	32-33	-0.19177972767756302
2216	34-35	-0.13905278442143043
2216	36-37	-0.1284925624506812
2216	38-39	-0.12296200313765837
2216	40-41	-0.10342480854201597
2216	42-43	-0.089018148226792
2216	44-45	-0.09493558634905241
2216	46-47	-0.13036027443076392
2216	48-49	-0.14436755844199922
2216	50-51	-0.1285596798251305
2216	52-53	-0.12742217233781616
2216	54-55	-0.07745543123093057
2216	56-57	-0.09579532857072337
2216	58-59	-0.2248270823480354
2216	60-61	-0.23970415183714522
2216	62-63	-0.20283287471139033
2216	64-65	-0.2038729050362207
2216	66-67	-0.17699701489835462
2216	68-69	-0.18122515835862885
2216	70-71	-0.18690127550482316
2216	72-73	-0.19805627552538851
2216	74-75	-0.20405331023790296
2216	76-77	-0.1973694371313215
2216	78-79	-0.13536520803923224
2216	80-81	-0.14293237931399005
2216	82-83	-0.17583023732599656
2216	84-85	-0.1352849582124378
2216	86-87	-0.13100620239689675
2216	88-89	-0.11922095995201687
2216	90-91	-0.15512533753739888
2216	92-93	-0.16285571055031056
2216	94-95	-0.15575995007073828
2216	96-97	-0.13646791443555983
2216	98-99	-0.18625953769426573
2216	100	-0.1535213468548129
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	45.0
3	211.0
4	78.0
5	119.0
6	324.0
7	757.0
8	1315.0
9	2346.0
10	3560.0
11	5613.0
12	8406.0
13	12599.0
14	14181.0
15	18905.0
16	24427.0
17	38079.0
18	43628.0
19	21808.0
20	13550.0
21	12444.0
22	12120.0
23	12256.0
24	13510.0
25	15754.0
26	19903.0
27	24931.0
28	30904.0
29	39792.0
30	50475.0
31	63687.0
32	80667.0
33	105271.0
34	145660.0
35	227575.0
36	380393.0
37	798694.0
38	1446458.0
39	920152.0
40	9042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.299020111897526	21.316879554834443	14.702716042434036	22.681384290833993
2	14.307331024826409	21.988853184674493	40.695184433770706	23.008631356728397
3	16.239301808017988	28.578646282169256	32.19606765883285	22.985984250979904
4	11.006531029805576	18.521577118904744	40.04912505068037	30.422766800609313
5	11.211891924199854	39.37484759801478	35.7440097086832	13.669250769102167
6	27.123223264848185	40.31633207703026	18.762505035566633	13.797939622554923
7	24.167278005922107	35.24154160097791	23.74936656305828	16.841813830041698
8	24.97320678087617	35.335704802907756	21.252504795288115	18.438583620927957
9	25.87769864218798	15.780543843478645	22.346404631704907	35.99535288262847
10-11	22.73756672328725	27.62206094458896	29.836660397057003	19.803711935066787
12-13	24.25357525269564	25.961262986270462	29.89935251427141	19.88580924676249
14-15	21.553580437083983	27.019278672995732	28.54420318819319	22.882937701727094
16-17	19.39579045029276	30.300462871665946	29.166965210917994	21.136781467123296
18-19	19.361878087974276	28.053756200842294	32.93865564472687	19.645710066456555
20-21	20.956540110601715	27.03809756563229	32.73250355709612	19.272858766669863
22-23	20.456022843353278	27.152414789300543	32.85041660199708	19.541145765349096
24-25	20.129694144865894	27.255339054705154	32.74350717523754	19.871459625191413
26-27	19.791485600695985	27.393182532032306	32.756952770923576	20.05837909634813
28-29	19.814517733335094	27.3053075861221	32.96033472788856	19.919839952654247
30-31	20.12496025113705	27.552883575715715	32.624990015431976	19.697166157715262
32-33	19.802648682921106	27.44667858261543	32.89586561892328	19.854807115540183
34-35	20.149653714457404	27.339402692615245	32.56509549099294	19.94584810193441
36-37	20.28370937528716	27.360176067706494	32.214257704666856	20.14185685233949
38-39	20.093742085336576	27.587080238722482	32.316244515205895	20.002933160735044
40-41	20.077016982202363	27.439315763457707	32.27985441692793	20.203812837412
42-43	20.11935861224589	27.534262647393458	32.01229411673025	20.334084623630403
44-45	20.390355937596734	27.645828778495247	31.661853871413943	20.301961412494073
46-47	20.5201668054403	27.985499282572956	31.044172660017296	20.450161251969448
48-49	20.653922004851683	28.026357502169812	30.575466646867117	20.744253846111384
50-51	20.64668257056013	28.14071319881371	30.299568785205118	20.913035445421038
52-53	20.625141922245767	28.30196378176758	30.280887530043138	20.792006765943512
54-55	20.69527030376446	28.550075450271766	30.176461203667245	20.578193042296526
56-57	20.67983219963386	28.238620017547213	30.070156381472863	21.011391401346067
58-59	20.618862100297484	27.961834419915544	30.45666758485434	20.96263589493263
60-61	20.743649050726418	28.156371189186906	30.247955173462554	20.852024586624125
62-63	20.556272911960708	28.089536181280355	30.421975134130015	20.932215772628926
64-65	20.575779321213144	27.957545786237446	30.54193414314984	20.924740749399565
66-67	20.52374853654136	28.042795280955378	30.342539967830696	21.090916214672568
68-69	20.87512241245399	28.230184335188895	29.998118896153542	20.896574356203573
70-71	20.80839444578414	28.006801849327196	30.04362257897068	21.141181125917985
72-73	20.69122157539354	28.124366156359176	30.16453904455181	21.019873223695477
74-75	20.685769936664574	28.292406903136097	29.9213537591535	21.100469401045824
76-77	20.73939711033517	28.199845809211034	29.87177573650022	21.188981343953586
78-79	20.77880119490865	28.19953026236038	30.026344272231363	20.99532427049961
80-81	20.707316421124204	28.238171625842618	29.939443910908764	21.115068042124413
82-83	20.59062856499631	28.168227778775943	30.16936641289124	21.071777243336502
84-85	20.567214455094412	28.159470496802143	29.911146597777222	21.362168450326227
86-87	20.702196329359893	28.161875559916734	29.83140018986704	21.304527920856334
88-89	20.545034869000965	28.06374437827194	29.976008620704587	21.415212132022507
90-91	20.4516034344218	28.0834919334728	29.846064061179533	21.61884057092587
92-93	20.469713086843715	28.15351851875904	29.8337664890317	21.543001905365546
94-95	20.38483368495062	28.014153155052618	29.991994988317185	21.609018171679576
96-97	20.33923914285813	28.150976095065584	29.787578317926624	21.722206444149663
98-99	20.260408695687936	28.024083350524492	29.83687196985555	21.87863598393202
100	20.291915279758456	28.1277193748068	29.735965753086614	21.84439959234813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2318.0
1	1350.0
2	1677.0
3	2945.5
4	1885.5
5	1144.0
6	2578.5
7	6825.0
8	12123.5
9	13568.0
10	9381.5
11	5386.0
12	4963.5
13	5322.5
14	5826.5
15	6435.0
16	7081.5
17	7756.0
18	8649.5
19	9734.5
20	11176.0
21	13547.5
22	17665.0
23	24511.5
24	31911.0
25	39114.0
26	49189.0
27	62458.5
28	79971.0
29	95255.5
30	103446.0
31	113834.5
32	125658.0
33	135818.5
34	145378.0
35	153184.5
36	156259.5
37	157776.5
38	159628.5
39	159068.0
40	159494.5
41	157256.0
42	152506.0
43	149447.0
44	147261.0
45	144208.5
46	141113.0
47	136714.5
48	130287.0
49	126477.0
50	126110.0
51	121597.5
52	111674.5
53	103974.0
54	98985.0
55	94946.0
56	87227.0
57	77177.5
58	70118.5
59	63818.0
60	55773.0
61	46434.5
62	38930.5
63	31378.5
64	23642.0
65	18512.5
66	14587.5
67	10831.5
68	7746.5
69	5666.0
70	4158.0
71	3111.0
72	2311.5
73	1654.5
74	1302.0
75	956.0
76	568.0
77	413.0
78	322.5
79	167.0
80	69.5
81	27.0
82	12.5
83	8.5
84	6.0
85	4.5
86	3.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00506533086243319
2	0.004394282756726229
3	0.0010823356543660661
4	0.0
5	0.04149674898839498
6	0.0
7	0.0
8	0.0
9	2.1646713087321324E-5
10-11	0.0
12-13	1.0823356543660662E-5
14-15	1.0823356543660662E-5
16-17	0.0
18-19	9.741020889294597E-5
20-21	0.0
22-23	4.329342617464265E-5
24-25	2.1646713087321324E-5
26-27	1.2988027852392796E-4
28-29	1.2988027852392796E-4
30-31	2.1646713087321323E-4
32-33	0.0012988027852392794
34-35	8.875152365801744E-4
36-37	0.0011364524370843696
38-39	0.001060688941278745
40-41	1.8399706124223126E-4
42-43	6.71048105706961E-4
44-45	1.9482041778589194E-4
46-47	1.1905692198026729E-4
48-49	1.1905692198026729E-4
50-51	3.355240528534805E-4
52-53	6.926948187942824E-4
54-55	8.658685234928529E-4
56-57	0.0022079647349067754
58-59	6.818714622506218E-4
60-61	0.0011689225067153515
62-63	0.0011689225067153515
64-65	0.001601856768461778
66-67	3.788174790281232E-4
68-69	2.3811384396053457E-4
70-71	2.814072701351772E-4
72-73	1.2988027852392796E-4
74-75	6.169313229886578E-4
76-77	8.117517407745498E-4
78-79	8.442218104055317E-4
80-81	8.117517407745498E-4
82-83	0.0014936232030251715
84-85	0.0013745662810449042
86-87	0.00195902753440258
88-89	0.0013637429245012436
90-91	0.0011905692198026729
92-93	0.0011472757936280304
94-95	7.143415318816037E-4
96-97	1.5152699161124927E-4
98-99	3.463474093971412E-4
100	2.3811384396053457E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4619639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.68935857809611
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4774962894555	39.637477834166624
2	16.192484154117835	17.71113144349379
3	5.353949418433953	8.784121786611708
4	2.2068329724077396	4.827611189998902
5	1.1029663516338895	3.0160261152040118
6	0.644842700423104	2.1159620201944165
7	0.41102174824930576	1.5734961041381563
8	0.27917110185168303	1.221415079504711
9	0.20285336873804163	0.9984528559520561
>10	1.0144035989628408	9.966057507310595
>50	0.06848393312852433	2.598227091780383
>100	0.041725655956789516	4.15361992321831
>500	0.002419906900837877	0.850207067375986
>1k	0.0012297879982938033	1.1665716629268412
>5k	0.0	0.0
>10k+	1.1901174177028563E-4	1.379622318123244
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26859	0.5814090668123635	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22100	0.4783923592298013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14631	0.3167130591805983	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4686	0.10143649752718774	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.1646713087321324E-5	0.0	0.0	0.0
3	0.0	2.1646713087321324E-5	0.0	0.0	0.0
4	0.0	2.1646713087321324E-5	0.0	0.0	0.0
5	0.0	2.1646713087321324E-5	0.0	0.0	0.0
6	0.0	2.1646713087321324E-5	0.0	0.0	0.0
7	0.0	2.1646713087321324E-5	0.0	0.0	0.0
8	0.0	2.1646713087321324E-5	0.0	0.0	0.0
9	0.0	2.1646713087321324E-5	0.0	2.1646713087321324E-5	0.0
10-11	0.0	2.1646713087321324E-5	0.0	2.1646713087321324E-5	0.0
12-13	0.0	2.1646713087321324E-5	0.0	2.1646713087321324E-5	0.0
14-15	0.0	2.1646713087321324E-5	0.0	2.1646713087321324E-5	0.0
16-17	0.0	2.1646713087321324E-5	0.0	2.1646713087321324E-5	0.0
18-19	0.0	2.1646713087321324E-5	0.0	2.1646713087321324E-5	0.0
20-21	0.0	2.1646713087321324E-5	0.0	6.494013926196398E-5	0.0
22-23	0.0	2.1646713087321324E-5	0.0	2.3811384396053457E-4	0.0
24-25	0.0	2.1646713087321324E-5	0.0	4.978744010083905E-4	0.0
26-27	0.0	2.1646713087321324E-5	0.0	8.766918800365137E-4	0.0
28-29	0.0	2.1646713087321324E-5	0.0	0.0024244318657799884	0.0
30-31	0.0	2.1646713087321324E-5	0.0	0.005822965820489436	0.0
32-33	0.0	2.1646713087321324E-5	0.0	0.012436036668666103	0.0
34-35	0.0	2.1646713087321324E-5	0.0	0.023140336290346494	0.0
36-37	0.0	2.1646713087321324E-5	0.0	0.040187122846612036	0.0
38-39	0.0	2.1646713087321324E-5	0.0	0.06489684583578934	0.0
40-41	0.0	2.1646713087321324E-5	0.0	0.09048326070500315	0.0
42-43	0.0	2.1646713087321324E-5	0.0	0.11613461571347891	0.0
44-45	0.0	2.1646713087321324E-5	0.0	0.1481933977958018	0.0
46-47	0.0	2.1646713087321324E-5	0.0	0.18859698777328704	0.0
48-49	0.0	2.1646713087321324E-5	0.0	0.233124276593907	0.0
50-51	0.0	2.1646713087321324E-5	0.0	0.28199173138853495	0.0
52-53	0.0	2.1646713087321324E-5	0.0	0.32809923026452936	0.0
54-55	0.0	2.1646713087321324E-5	0.0	0.38028945551806104	0.0
56-57	1.0823356543660662E-5	2.1646713087321324E-5	0.0	0.4444719598219688	0.0
58-59	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.5172482092215431	0.0
60-61	2.1646713087321324E-5	2.1646713087321324E-5	0.0	0.5971570505833897	0.0
62-63	4.329342617464265E-5	2.1646713087321324E-5	0.0	0.6780616407472533	0.0
64-65	4.329342617464265E-5	2.1646713087321324E-5	0.0	0.7655143616200313	0.0
66-67	4.329342617464265E-5	2.1646713087321324E-5	0.0	0.8661282840499009	0.0
68-69	4.329342617464265E-5	2.1646713087321324E-5	0.0	0.9835292324789882	0.0
70-71	4.329342617464265E-5	3.247006963098199E-5	0.0	1.118096024386321	1.0823356543660662E-5
72-73	4.329342617464265E-5	4.329342617464265E-5	0.0	1.2602499892307604	2.1646713087321324E-5
74-75	4.329342617464265E-5	4.329342617464265E-5	0.0	1.4017329059694923	2.1646713087321324E-5
76-77	4.329342617464265E-5	4.329342617464265E-5	0.0	1.5605331931780817	2.1646713087321324E-5
78-79	4.329342617464265E-5	4.329342617464265E-5	0.0	1.7452445959521947	2.1646713087321324E-5
80-81	4.329342617464265E-5	4.329342617464265E-5	0.0	1.9529772780946737	2.1646713087321324E-5
82-83	6.494013926196398E-5	4.329342617464265E-5	0.0	2.177312989175128	2.1646713087321324E-5
84-85	6.494013926196398E-5	4.329342617464265E-5	0.0	2.406692384404929	2.1646713087321324E-5
86-87	8.65868523492853E-5	4.329342617464265E-5	0.0	2.6482155856767164	2.1646713087321324E-5
88	8.65868523492853E-5	4.329342617464265E-5	0.0	2.8482528613166527	2.1646713087321324E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12050	0.0	64.58378	1
GTATCAA	23960	0.0	45.374672	1
ATCAACG	31230	0.0	34.566917	3
TCAACGC	31540	0.0	34.22717	4
TATCAAC	32065	0.0	33.814865	2
CAACGCA	32655	0.0	33.058487	5
GTGGTAT	3845	0.0	32.53103	1
AACGCAG	33925	0.0	31.91832	6
TGGTATC	4085	0.0	29.58153	2
CGCAGAG	38170	0.0	28.171583	8
ACGCAGA	38300	0.0	28.125046	7
TATAACG	460	0.0	26.576334	2
TACCTGG	3500	0.0	26.331015	2
GTACATG	23780	0.0	26.141598	1
GCAGAGT	41100	0.0	26.003155	9
TACATGG	24110	0.0	25.216272	2
CATGGGT	10815	0.0	24.95434	4
ACATGGG	24070	0.0	24.61247	3
ATAACGC	535	0.0	24.607359	3
GAGTACT	27130	0.0	23.135107	12-13
>>END_MODULE