FastQCFastQC Report
Wed 25 May 2016
SRR2431390_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431390_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296883
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA238870.7245328390482768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51100.050.454631
GTATCAA93250.039.8583761
TCAACGC109050.032.8738754
ATCAACG109150.032.8447533
CAACGCA117150.030.7213785
TATCAAC119250.030.6152732
TACCTGG28950.029.9022622
AACGCAG122650.029.2337066
GTACATG202300.028.4892861
TACATGG199700.028.0116422
CATGGGT95700.027.431144
GTACCTG33000.026.8031181
ACATGGG206450.026.0475043
ACGCAGA141300.025.2436927
CGCAGAG139100.025.2375228
ACCTGGG35650.023.6226313
ATGGGTG52000.023.1610475
GCAGAGT152350.022.209739
GTGGTAT25500.022.1402471
TGGTATC25500.020.8484152
Produced by FastQC (version 0.11.5)