##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431390_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3296883
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59281114919759	33.0	31.0	34.0	26.0	34.0
2	30.721967082241015	33.0	31.0	34.0	26.0	34.0
3	30.765631355434817	34.0	31.0	34.0	26.0	34.0
4	33.90381217653159	37.0	35.0	37.0	28.0	37.0
5	34.06379025279332	37.0	35.0	37.0	30.0	37.0
6	34.006426373031736	37.0	35.0	37.0	30.0	37.0
7	33.981553485519505	37.0	35.0	37.0	30.0	37.0
8	33.93063539106483	37.0	35.0	37.0	30.0	37.0
9	35.31590171686408	39.0	37.0	39.0	27.0	39.0
10-11	35.362093073973206	39.0	37.0	39.0	28.0	39.0
12-13	35.303028800233434	39.0	37.0	39.0	27.5	39.0
14-15	36.6285162379132	40.5	38.0	41.0	27.5	41.0
16-17	36.65180459846467	40.5	38.0	41.0	28.0	41.0
18-19	36.67181804753156	40.0	38.0	41.0	28.0	41.0
20-21	36.762795343359166	40.5	38.0	41.0	28.5	41.0
22-23	36.74066140654673	40.0	38.0	41.0	28.0	41.0
24-25	36.79934607931189	40.0	38.0	41.0	29.0	41.0
26-27	36.85498105331612	40.0	38.0	41.0	29.5	41.0
28-29	36.83452081860351	40.0	38.0	41.0	30.0	41.0
30-31	36.766349002982516	40.0	38.0	41.0	30.0	41.0
32-33	36.71318924572088	40.0	37.5	41.0	30.0	41.0
34-35	36.63885964409414	40.0	37.0	41.0	29.5	41.0
36-37	36.50164382539508	40.0	37.0	41.0	29.0	41.0
38-39	36.376099940458914	40.0	37.0	41.0	28.0	41.0
40-41	36.317610452054254	40.0	37.0	41.0	28.0	41.0
42-43	36.14232139872722	40.0	36.5	41.0	27.0	41.0
44-45	35.98280042088239	40.0	36.0	41.0	27.0	41.0
46-47	35.8488677032215	40.0	36.0	41.0	27.0	41.0
48-49	35.71268740807605	40.0	35.0	41.0	27.0	41.0
50-51	35.57955863159232	40.0	35.0	41.0	27.0	41.0
52-53	35.41746522397064	40.0	35.0	41.0	26.5	41.0
54-55	35.25402751629342	39.0	35.0	41.0	26.0	41.0
56-57	35.06531942443817	39.0	35.0	41.0	26.0	41.0
58-59	34.848369960353466	39.0	34.0	41.0	26.0	41.0
60-61	34.60176081468466	38.5	34.0	40.5	25.5	41.0
62-63	34.49018618495106	38.0	34.0	40.5	25.5	41.0
64-65	34.227396301294284	38.0	34.0	40.0	24.5	41.0
66-67	33.95100857385597	37.0	34.0	40.0	23.5	41.0
68-69	33.63999571716679	37.0	33.5	40.0	23.0	41.0
70-71	33.29960086542349	36.5	33.0	39.5	22.0	41.0
72-73	32.94999777062152	36.0	33.0	39.0	21.5	41.0
74-75	32.65987161206509	35.5	33.0	39.0	20.0	41.0
76-77	32.396774013515184	35.0	33.0	38.5	20.0	40.0
78-79	32.05140673781872	35.0	32.5	37.0	18.5	39.5
80-81	31.728149740224325	35.0	32.0	37.0	18.0	39.0
82-83	31.411596195558047	35.0	32.0	36.5	16.0	39.0
84-85	31.118661475096324	35.0	32.0	36.0	12.0	37.5
86-87	30.849352251808753	35.0	32.0	36.0	7.5	37.0
88-89	30.591842203681477	35.0	31.5	35.0	4.0	37.0
90-91	30.367580529851985	35.0	31.0	35.0	2.0	36.0
92-93	30.146316687610692	35.0	31.0	35.0	2.0	36.0
94-95	29.937530388551853	35.0	31.0	35.0	2.0	36.0
96-97	29.747153599323966	35.0	31.0	35.0	2.0	36.0
98-99	29.53404564250536	35.0	31.0	35.0	2.0	35.0
100	29.363003479347007	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.23565063444731038
1101	2	0.29198180117170836
1101	3	0.32191770946472786
1101	4	0.36225668189074156
1101	5	0.3182790095751855
1101	6	0.3995292066712537
1101	7	0.4027677434704344
1101	8	0.41021030633545763
1101	9	0.3427096794657629
1101	10-11	0.4020860383031888
1101	12-13	0.24686122705779212
1101	14-15	0.4010274247081682
1101	16-17	0.3492816984079923
1101	18-19	0.2751457443954095
1101	20-21	0.24572366785548638
1101	22-23	0.3738163532012777
1101	24-25	0.35142880919625696
1101	26-27	0.30127514200598426
1101	28-29	0.3687718126569308
1101	30-31	0.2961296005826881
1101	32-33	0.2708190141422975
1101	34-35	0.34104647746156047
1101	36-37	0.283494681209028
1101	38-39	0.33969004604656305
1101	40-41	0.29116705184686964
1101	42-43	0.2732599582110211
1101	44-45	0.27529983271445957
1101	46-47	0.3525845855593772
1101	48-49	0.2740808452742911
1101	50-51	0.22994546692718387
1101	52-53	0.23230329263590477
1101	54-55	0.14935133308608783
1101	56-57	0.13525986749996832
1101	58-59	0.07828681849917274
1101	60-61	0.028253034317117454
1101	62-63	0.13086955999535377
1101	64-65	0.16393750456554557
1101	66-67	0.22885677017989536
1101	68-69	0.236175047949871
1101	70-71	0.2560046269280747
1101	72-73	0.1741321316528186
1101	74-75	0.19140207055766467
1101	76-77	0.19541216788584848
1101	78-79	0.13266420081679797
1101	80-81	0.05601518202370315
1101	82-83	0.12780042406532743
1101	84-85	0.14035349030795885
1101	86-87	0.11051616946425113
1101	88-89	0.05971318085759236
1101	90-91	0.08579564422710462
1101	92-93	0.08241224925028945
1101	94-95	0.08698840254648132
1101	96-97	0.1042805946690315
1101	98-99	0.08284582254386663
1101	100	0.1126646744690376
1102	1	0.24390804277268785
1102	2	0.19034429952116483
1102	3	0.24453129392602335
1102	4	0.2999038328178969
1102	5	0.3072571661181627
1102	6	0.33281251861065897
1102	7	0.2535420017720398
1102	8	0.24693194422314235
1102	9	0.3393828869753577
1102	10-11	0.2951670675497482
1102	12-13	0.2275616817059074
1102	14-15	0.2669344624819914
1102	16-17	0.20233663824161852
1102	18-19	0.3146540321087983
1102	20-21	0.2502508926259779
1102	22-23	0.1987583406506559
1102	24-25	0.1730277692996438
1102	26-27	0.17775607651313408
1102	28-29	0.17387273212699483
1102	30-31	0.22331370276269524
1102	32-33	0.21651748092254053
1102	34-35	0.22142621253757966
1102	36-37	0.19999191911892922
1102	38-39	0.19704627557711518
1102	40-41	0.23246240030820786
1102	42-43	0.16323395177624178
1102	44-45	0.06641059086778256
1102	46-47	0.07052796126929906
1102	48-49	0.05026631442915175
1102	50-51	-0.017667468139883624
1102	52-53	-0.007170432873813581
1102	54-55	-0.001288239049706874
1102	56-57	0.0465716023422047
1102	58-59	0.05064382008730206
1102	60-61	0.09876827271334321
1102	62-63	0.09476603717600796
1102	64-65	0.09651865900087131
1102	66-67	0.072544063050195
1102	68-69	0.048164191160779524
1102	70-71	-0.014118566763855256
1102	72-73	-0.11574112463349451
1102	74-75	-0.11574241135923558
1102	76-77	-0.07923347031353956
1102	78-79	-0.05553195881007866
1102	80-81	-0.08312014596006634
1102	82-83	-0.11349620514572223
1102	84-85	-0.12059723546223822
1102	86-87	-0.07356364252890657
1102	88-89	-0.01822310022043183
1102	90-91	-0.04647022029338643
1102	92-93	-0.0805574251218033
1102	94-95	0.0050719662343787775
1102	96-97	0.013893847939275616
1102	98-99	-0.07933966320662122
1102	100	-0.04487800335855141
1103	1	-0.2344939498967662
1103	2	-0.12127989242670623
1103	3	-0.07433489493590528
1103	4	0.0185493309565814
1103	5	0.029386537629982
1103	6	0.044448325859754334
1103	7	0.03201633912627244
1103	8	7.685785964000047E-4
1103	9	0.0687090847853753
1103	10-11	0.037597762919112654
1103	12-13	0.05659251387771036
1103	14-15	0.05033311395985862
1103	16-17	0.02993939780775179
1103	18-19	0.022961015916202143
1103	20-21	0.010164588069912384
1103	22-23	-0.02895193215118752
1103	24-25	0.0967193629763301
1103	26-27	0.02672973559113956
1103	28-29	-0.08138372942205763
1103	30-31	-0.052433727531152385
1103	32-33	0.0023004956237784313
1103	34-35	-0.048942329498345316
1103	36-37	-0.11340808747740994
1103	38-39	-0.09246277718789742
1103	40-41	-0.04507751474235988
1103	42-43	-0.13657991660662105
1103	44-45	-0.2727097795025841
1103	46-47	-0.346553127873122
1103	48-49	-0.2577924460891481
1103	50-51	-0.42061696595735754
1103	52-53	-0.4676201529593911
1103	54-55	-0.3804733324596725
1103	56-57	-0.4278439042283324
1103	58-59	-0.3832893144953218
1103	60-61	-0.4372771617562847
1103	62-63	-0.3501889069271513
1103	64-65	-0.3948801028940565
1103	66-67	-0.37325137715537693
1103	68-69	-0.3935924223909737
1103	70-71	-0.3939973517746651
1103	72-73	-0.37907344700542467
1103	74-75	-0.30315475885463883
1103	76-77	-0.29442144460500685
1103	78-79	-0.36418672050998424
1103	80-81	-0.3916474619937702
1103	82-83	-0.37842473519350506
1103	84-85	-0.4370168618797372
1103	86-87	-0.498578269204522
1103	88-89	-0.5341690446161209
1103	90-91	-0.49810736832108304
1103	92-93	-0.5736583305417007
1103	94-95	-0.5281097945879303
1103	96-97	-0.4644136807835011
1103	98-99	-0.5463249619636414
1103	100	-0.4793078354672602
1104	1	-0.16648150197416456
1104	2	-0.12376170315130963
1104	3	-0.08392928922001985
1104	4	-0.05828259394785107
1104	5	-0.10215760908862848
1104	6	-0.023858717269689578
1104	7	-0.04127657398590401
1104	8	-0.1097973115348907
1104	9	-0.0101476325481471
1104	10-11	-0.11091779381833788
1104	12-13	-0.043068033299029196
1104	14-15	-0.0697620956934415
1104	16-17	-0.04919256818170936
1104	18-19	-0.08018534003782207
1104	20-21	-0.12042142071553741
1104	22-23	-0.0602490673842766
1104	24-25	-0.02370891513842821
1104	26-27	-0.02863811445128306
1104	28-29	-0.01007555258473758
1104	30-31	-0.03819180805783162
1104	32-33	-0.08198081870350649
1104	34-35	0.028067539704906608
1104	36-37	-0.04963838213593874
1104	38-39	-0.05387990676477017
1104	40-41	-0.01998622540378392
1104	42-43	-0.15567012570414107
1104	44-45	-0.06932384289736859
1104	46-47	-0.22022050227179335
1104	48-49	-0.23728954305124716
1104	50-51	-0.26675498987954427
1104	52-53	-0.3185068317686657
1104	54-55	-0.18319857555787422
1104	56-57	-0.21254361410041867
1104	58-59	-0.2628207603791495
1104	60-61	-0.3067780805415694
1104	62-63	-0.24220015325306576
1104	64-65	-0.25511864059028255
1104	66-67	-0.1831152776873708
1104	68-69	-0.1451170625099536
1104	70-71	-0.17429765597280067
1104	72-73	-0.21079129936943275
1104	74-75	-0.11987094389770903
1104	76-77	-0.16685358569834818
1104	78-79	-0.1710081560662715
1104	80-81	-0.20866474971989746
1104	82-83	-0.2408529067263565
1104	84-85	-0.24836701721495302
1104	86-87	-0.18549074962933076
1104	88-89	-0.30204481795052374
1104	90-91	-0.3570045744680783
1104	92-93	-0.40123751597452184
1104	94-95	-0.3722718321526024
1104	96-97	-0.3041527010882348
1104	98-99	-0.36232378569334855
1104	100	-0.30020212802369173
1105	1	-0.08118850741221806
1105	2	-0.09458257095326417
1105	3	-0.14888952079650153
1105	4	0.005869673687371346
1105	5	-0.05178274223332835
1105	6	-0.15477650955911315
1105	7	-0.14224807114560178
1105	8	-0.15949701263934202
1105	9	-0.244183522883354
1105	10-11	-0.2316337311258181
1105	12-13	-0.20977922590820697
1105	14-15	-0.20481789865384314
1105	16-17	-0.21036790406671457
1105	18-19	-0.13130081884936118
1105	20-21	-0.13196724016266614
1105	22-23	-0.16929032855301784
1105	24-25	-0.12236670918809978
1105	26-27	-0.04138141321030986
1105	28-29	-0.1250244274470873
1105	30-31	-0.11225928409178465
1105	32-33	-0.10739408701856235
1105	34-35	-0.17410094299650325
1105	36-37	-0.09966838950263934
1105	38-39	-0.15805181432544657
1105	40-41	-0.11856267731334214
1105	42-43	-0.17437704209031324
1105	44-45	-0.25624695473752723
1105	46-47	-0.21446106294524014
1105	48-49	-0.3224926194646969
1105	50-51	-0.25309912586489247
1105	52-53	-0.2405248843529293
1105	54-55	-0.2434909464101196
1105	56-57	-0.2969861978109165
1105	58-59	-0.2644563957556869
1105	60-61	-0.27300697229952675
1105	62-63	-0.12826506653316727
1105	64-65	-0.14251032300562372
1105	66-67	-0.19691870968465253
1105	68-69	-0.08153371341062865
1105	70-71	-0.07180465364913147
1105	72-73	-0.16577912317006138
1105	74-75	-0.11567831531476003
1105	76-77	-0.14099821981984206
1105	78-79	-0.22243964779276126
1105	80-81	-0.20899224062586086
1105	82-83	-0.16871256372760257
1105	84-85	-0.19458790816347005
1105	86-87	-0.16390692893616432
1105	88-89	-0.20681591129283916
1105	90-91	-0.2367798518629698
1105	92-93	-0.22030357653396493
1105	94-95	-0.2342633701444896
1105	96-97	-0.24355930367912393
1105	98-99	-0.2025576872262178
1105	100	-0.2003082626244037
1106	1	-0.02478348679828457
1106	2	-0.027776503809196385
1106	3	0.1047885091340035
1106	4	0.01475986541603902
1106	5	0.13795056764551106
1106	6	-0.07125201701605022
1106	7	0.022325011407126283
1106	8	-0.07802698522056062
1106	9	-0.06439175926411167
1106	10-11	-0.03223522150017999
1106	12-13	-0.04543570338766756
1106	14-15	-0.1419206254586456
1106	16-17	-0.04192951892564167
1106	18-19	-0.0579711532381495
1106	20-21	-0.12892614004542935
1106	22-23	-0.025794101381016787
1106	24-25	0.01678667645484211
1106	26-27	-0.040928904508660935
1106	28-29	-0.09494152307079418
1106	30-31	-0.04127270232768865
1106	32-33	-0.06115571583529089
1106	34-35	-0.02471984258862392
1106	36-37	-0.08047625831697758
1106	38-39	-0.06345301462785358
1106	40-41	-0.08098377608548901
1106	42-43	-0.04578039740125206
1106	44-45	-0.06925019983936664
1106	46-47	-0.04691916169137045
1106	48-49	-0.12193809300048741
1106	50-51	-0.10975595279605699
1106	52-53	-0.09620156091848031
1106	54-55	-0.13002479608032047
1106	56-57	-0.15163156515935583
1106	58-59	-0.24290949994468036
1106	60-61	-0.2117911608601517
1106	62-63	-0.22018077246109868
1106	64-65	-0.10955094778066155
1106	66-67	-0.06169967629376316
1106	68-69	-0.08232354946911613
1106	70-71	-0.11372052433307545
1106	72-73	-0.11835199517305739
1106	74-75	-0.08038517129367762
1106	76-77	-0.1017485237313025
1106	78-79	-0.07412597618747441
1106	80-81	-0.11592750942823926
1106	82-83	-0.15215788044947232
1106	84-85	-0.1284144195843524
1106	86-87	-0.09853622454764022
1106	88-89	-0.16130605736297454
1106	90-91	-0.11596249986201812
1106	92-93	-0.11477047004007446
1106	94-95	-0.14224281180582565
1106	96-97	-0.15745781600844921
1106	98-99	-0.10259755817917693
1106	100	-0.1053223708077553
1107	1	-0.2723149369802158
1107	2	-0.3878295017789597
1107	3	-0.3328637662191021
1107	4	-0.2309029315806299
1107	5	-0.1386941077522863
1107	6	-0.319171767923109
1107	7	-0.285529713122223
1107	8	-0.21005926580214407
1107	9	-0.2717346026827272
1107	10-11	-0.23890968182332273
1107	12-13	-0.22330687420453899
1107	14-15	-0.3269778474685978
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2216	52-53	0.2730243555899179
2216	54-55	0.2780252057096888
2216	56-57	0.3096429153314446
2216	58-59	0.2844052018424321
2216	60-61	0.2909713674264083
2216	62-63	0.3080497668809201
2216	64-65	0.25952763102880283
2216	66-67	0.24183446046159673
2216	68-69	0.2507802036320044
2216	70-71	0.27745546224932127
2216	72-73	0.27921652567511757
2216	74-75	0.3188919987570742
2216	76-77	0.31791072944869114
2216	78-79	0.32237117756926637
2216	80-81	0.3022267037442745
2216	82-83	0.30153245833130526
2216	84-85	0.2789315456834416
2216	86-87	0.24578259926666846
2216	88-89	0.29163720890365497
2216	90-91	0.3294741257417293
2216	92-93	0.24872737407265078
2216	94-95	0.21767920453736878
2216	96-97	0.21996382799347103
2216	98-99	0.17374645205795147
2216	100	0.1426015010773085
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89665.0
3	17694.0
4	12588.0
5	10986.0
6	7412.0
7	6305.0
8	7021.0
9	7346.0
10	7169.0
11	7837.0
12	9317.0
13	10642.0
14	11615.0
15	12223.0
16	11849.0
17	11499.0
18	10857.0
19	9813.0
20	9479.0
21	10215.0
22	12171.0
23	18633.0
24	29856.0
25	38506.0
26	40444.0
27	30043.0
28	29303.0
29	36215.0
30	42401.0
31	48162.0
32	61916.0
33	83196.0
34	117232.0
35	188817.0
36	313861.0
37	585645.0
38	897031.0
39	440429.0
40	1490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.801491863364575	21.714943976118516	13.743429644858974	21.740134515657942
2	15.004056406843933	22.951119537903857	40.11233360376046	21.932490451491756
3	17.083932256133323	30.253993916058626	30.15902729872395	22.5030465290841
4	11.579776435579603	19.800738570256094	39.16540284835426	29.454082145810045
5	11.508668175056261	41.21647720624771	34.59219537545377	12.68265924324226
6	26.538652659556867	41.50562466931789	19.00457969108713	12.951142980038108
7	24.593954417256857	36.209901243764556	22.93431661553247	16.261827723446114
8	24.262318766678565	37.407425618761096	20.928518008600616	17.401737605959728
9	25.297479760814312	19.206572932391985	21.1689408245484	34.327006482245295
10-11	22.044088419675496	30.086775978345088	28.996638995993962	18.872496605985457
12-13	22.925260397734785	28.591412509259946	28.30549064383339	20.17783644917188
14-15	20.9549709684479	29.817994675398747	26.122144082337968	23.104890273815386
16-17	19.921618131513327	32.74163986682003	26.7103426641974	20.62639933746924
18-19	19.99555621254538	31.520324712163077	28.719099513824155	19.765019561467383
20-21	22.018692683803398	30.363940015371334	27.916061101660855	19.70130619916441
22-23	21.47701467004057	30.453147188946684	28.047640562530894	20.022197578481858
24-25	20.87881183971492	30.5899975868909	28.081305082250594	20.449885491143586
26-27	20.523734931036667	30.601532208260966	28.195187857681326	20.67954500302104
28-29	20.59320171511238	30.460015213335765	28.44107624940886	20.50570682214299
30-31	20.852335922056604	30.92882285944641	27.957892570412785	20.2609486480842
32-33	20.456686135016106	30.608710459879422	28.420820499713606	20.513782905390865
34-35	20.81688883060842	30.317986749692054	28.33488747922999	20.530236940469536
36-37	20.93761052073957	30.456669798561958	27.950222597307583	20.655497083390888
38-39	20.77213809043673	30.57538795038012	28.17324430624884	20.479229652934315
40-41	20.635917046722724	30.29185785830057	28.353241850732484	20.718983244244214
42-43	20.47237091123348	30.39246057036577	28.386027855467756	20.749140662932994
44-45	20.728186590489685	30.39849163994689	28.27073065326496	20.602591116298463
46-47	20.751158431051252	30.495852051101043	28.15736114650561	20.595628371342094
48-49	20.616263446164645	30.322531877056353	28.224787511589035	20.836417165189967
50-51	20.669972003548693	30.249721667158695	28.134968850248203	20.9453374790444
52-53	20.707474766056404	30.315125855911113	28.122393638440137	20.85500573959235
54-55	20.76962307366555	30.63691792559223	28.02991368018105	20.56354532056118
56-57	20.719018380698483	30.361732692539732	27.910886889001706	21.008362037760076
58-59	20.724680401004452	30.018262799745486	28.23216037749596	21.024896421754104
60-61	20.897843954192563	30.437219679894362	27.887302779864765	20.77763358604831
62-63	20.66462123306985	30.39892632948378	28.02657343633755	20.90987900110882
64-65	20.743776190237693	30.193616146162967	28.1032083183458	20.959399345253544
66-67	20.605124117361008	30.226701092951576	28.000517244205437	21.167657545481973
68-69	21.06663922650307	30.499681129514837	27.788249964106203	20.64542967987589
70-71	20.98642035245862	30.253434446280952	27.895530245785565	20.86461495547486
72-73	20.8035282446388	30.3630151673787	28.01127700365697	20.82217958432553
74-75	20.840514506931868	30.39354450182638	27.922512531514705	20.843428459727047
76-77	20.84544319395857	30.396581145210718	27.781458355815214	20.976517305015495
78-79	21.002438649776018	30.40714584485525	27.890212800574055	20.700202704794677
80-81	20.838492040905383	30.398102718803955	27.944128863338836	20.81927637695183
82-83	20.80171662198131	30.36221245944318	28.12405893609994	20.71201198247557
84-85	20.820611645833075	30.27826134736578	27.925388564544356	20.975738442256784
86-87	20.99226978202698	30.331945546966427	27.799688241356442	20.87609642965016
88-89	20.87411650171258	30.232239039581998	27.92748597589241	20.96615848281301
90-91	20.79330398374718	30.237895572442152	27.829828053721734	21.138972390088938
92-93	20.893629369353672	30.364221193507134	27.749370239996306	20.992779197142884
94-95	20.837259674887537	30.128502727937885	27.9589463902421	21.075291206932476
96-97	20.814174044301932	30.35678241108213	27.791043879109882	21.037999665506053
98-99	20.685517801612292	30.376015877622343	27.747483756279223	21.190982564486145
100	20.73435331740728	30.414517666052514	27.68993552770469	21.16119348883552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22200.0
1	16271.0
2	9443.0
3	7229.0
4	4923.0
5	3445.5
6	2816.0
7	2902.0
8	3406.5
9	3542.0
10	2676.0
11	1831.5
12	1706.0
13	1684.0
14	1685.5
15	1800.5
16	2039.5
17	2379.5
18	2914.5
19	3653.5
20	4708.0
21	6472.5
22	9342.5
23	13467.5
24	18283.5
25	23590.0
26	30610.5
27	39887.0
28	51593.0
29	62555.5
30	70146.5
31	77729.5
32	85877.5
33	93824.5
34	100394.0
35	105992.0
36	110359.0
37	113273.0
38	115271.0
39	115380.0
40	116282.5
41	115726.0
42	113346.0
43	112278.0
44	110245.0
45	106832.5
46	104502.0
47	102141.5
48	99284.0
49	97274.0
50	96154.5
51	91312.0
52	82331.0
53	75004.0
54	70438.0
55	68125.0
56	62361.0
57	54099.0
58	48785.0
59	44405.5
60	38846.0
61	32295.0
62	26406.5
63	20850.0
64	15999.5
65	12590.5
66	10020.5
67	7730.0
68	5683.0
69	4247.5
70	3230.5
71	2478.5
72	1882.5
73	1456.0
74	1248.5
75	1025.0
76	739.0
77	556.5
78	483.5
79	377.5
80	246.0
81	183.5
82	157.0
83	132.0
84	109.5
85	85.5
86	84.0
87	83.5
88	69.5
89	57.0
90	47.5
91	38.5
92	37.5
93	37.0
94	31.5
95	25.5
96	22.0
97	26.0
98	33.0
99	36.5
100	40.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18092847092238334
2	0.1757114219703884
3	0.18966399474897958
4	0.1798668621240123
5	0.19406208834223113
6	0.07752777396104138
7	0.07916568467852818
8	0.06779130469598102
9	0.06178563206519612
10-11	0.07523773212455523
12-13	0.07435811340590491
14-15	0.07170409140997724
16-17	0.06804912397558542
18-19	0.07267470516848792
20-21	0.07397896740648668
22-23	0.07217423244925586
24-25	0.07212873492932567
26-27	0.07545005388422944
28-29	0.07294769028806906
30-31	0.07871070947922629
32-33	0.07510123956476467
34-35	0.07488891780509044
36-37	0.08547467410884765
38-39	0.08127373643529358
40-41	0.07651166268260051
42-43	0.07972682075766717
44-45	0.09022158202156401
46-47	0.08081876123599169
48-49	0.08556566914870804
50-51	0.08236567691361811
52-53	0.0806671028362244
54-55	0.08304813971257094
56-57	0.07675431612222818
58-59	0.08356377827177973
60-61	0.0806216053162942
62-63	0.07249271508876719
64-65	0.07516190292467158
66-67	0.07585953156360115
68-69	0.07406996244634705
70-71	0.06926239117372379
72-73	0.06713917357698165
74-75	0.07246238340881372
76-77	0.07775526156069232
78-79	0.07391830404657976
80-81	0.08175904331454892
82-83	0.08307847139252439
84-85	0.0850348647495225
86-87	0.0809249221158288
88-89	0.0829268129927571
90-91	0.08145572651501433
92-93	0.07200740820951183
94-95	0.0716282622100936
96-97	0.07140077461044265
98-99	0.08150122403494453
100	0.0882651886645659
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3296883.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.2166697197422
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.23249827473774	49.45598004784397
2	13.726772298394637	17.355216614114408
3	4.012716837588229	7.610117850019904
4	1.552099275698254	3.924741891362705
5	0.7267156153726251	2.297027051859523
6	0.4223062343668974	1.601807624313612
7	0.2729527914771394	1.2078616527524393
8	0.17258385862270803	0.8728141431608333
9	0.12621846539238768	0.7181199935318955
>10	0.6681545849323774	7.709170005800122
>50	0.05624536598536024	2.4876636780846337
>100	0.02939411497541489	3.255552411211377
>500	9.587731836684276E-4	0.40272611951177983
>1k	3.355706135560826E-4	0.37730003604012163
>5k	0.0	0.0
>10k+	4.793865907944037E-5	0.7239008803928021
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	23887	0.7245328390482768	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.033167995345907E-5	0.0
6	0.0	0.0	0.0	3.033167995345907E-5	0.0
7	0.0	0.0	0.0	3.033167995345907E-5	0.0
8	0.0	0.0	0.0	3.033167995345907E-5	0.0
9	3.033167995345907E-5	0.0	0.0	6.066335990691814E-5	0.0
10-11	3.033167995345907E-5	0.0	0.0	6.066335990691814E-5	0.0
12-13	3.033167995345907E-5	0.0	0.0	6.066335990691814E-5	0.0
14-15	3.033167995345907E-5	0.0	0.0	7.582919988364767E-5	0.0
16-17	3.033167995345907E-5	0.0	0.0	9.099503986037721E-5	0.0
18-19	3.033167995345907E-5	0.0	0.0	1.2132671981383629E-4	0.0
20-21	3.033167995345907E-5	0.0	0.0	2.123217596742135E-4	0.0
22-23	3.033167995345907E-5	0.0	0.0	6.217994390459109E-4	0.0
24-25	3.033167995345907E-5	0.0	0.0	0.0012284330381150924	0.0
26-27	3.033167995345907E-5	0.0	0.0	0.002017056716905028	0.0
28-29	3.033167995345907E-5	0.0	0.0	0.004079610953740245	0.0
30-31	3.033167995345907E-5	0.0	0.0	0.008204719427410679	0.0
32-33	3.033167995345907E-5	0.0	0.0	0.016682423974402488	0.0
34-35	3.033167995345907E-5	0.0	0.0	0.02964921715450624	0.0
36-37	3.033167995345907E-5	0.0	0.0	0.05113921240153199	0.0
38-39	3.033167995345907E-5	0.0	0.0	0.07983298163750427	0.0
40-41	3.033167995345907E-5	0.0	0.0	0.11265185934714698	0.0
42-43	3.033167995345907E-5	0.0	0.0	0.14533424449699914	0.0
44-45	3.033167995345907E-5	0.0	0.0	0.18450760915689152	0.0
46-47	3.033167995345907E-5	0.0	0.0	0.2320373516439619	0.0
48-49	3.033167995345907E-5	0.0	0.0	0.28526944996228254	0.0
50-51	3.033167995345907E-5	0.0	0.0	0.34168637467571644	0.0
52-53	3.033167995345907E-5	0.0	0.0	0.3964805545116402	0.0
54-55	3.033167995345907E-5	0.0	0.0	0.4585998350563244	0.0
56-57	3.033167995345907E-5	0.0	0.0	0.5349143418192275	0.0
58-59	3.033167995345907E-5	0.0	0.0	0.622861047844282	0.0
60-61	3.033167995345907E-5	0.0	0.0	0.7170409140997724	0.0
62-63	3.033167995345907E-5	0.0	0.0	0.8111297853154025	0.0
64-65	3.033167995345907E-5	0.0	0.0	0.9114973142813985	0.0
66-67	3.033167995345907E-5	0.0	0.0	1.032202234656189	0.0
68-69	3.033167995345907E-5	0.0	0.0	1.169680574045242	0.0
70-71	4.5497519930188606E-5	0.0	0.0	1.325630906525952	0.0
72-73	6.066335990691814E-5	0.0	0.0	1.4947148564265094	0.0
74-75	6.066335990691814E-5	0.0	0.0	1.6632528360879049	0.0
76-77	6.066335990691814E-5	0.0	0.0	1.8469718215660063	0.0
78-79	6.066335990691814E-5	0.0	0.0	2.059976044039173	0.0
80-81	9.099503986037721E-5	0.0	0.0	2.301871191668009	0.0
82-83	9.099503986037721E-5	0.0	0.0	2.5606762508708982	0.0
84-85	9.099503986037721E-5	0.0	0.0	2.816948614797674	0.0
86-87	9.099503986037721E-5	0.0	0.0	3.0886143063008302	0.0
88	9.099503986037721E-5	0.0	0.0	3.3110971787594523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5110	0.0	50.45463	1
GTATCAA	9325	0.0	39.858376	1
TCAACGC	10905	0.0	32.873875	4
ATCAACG	10915	0.0	32.844753	3
CAACGCA	11715	0.0	30.721378	5
TATCAAC	11925	0.0	30.615273	2
TACCTGG	2895	0.0	29.902262	2
AACGCAG	12265	0.0	29.233706	6
GTACATG	20230	0.0	28.489286	1
TACATGG	19970	0.0	28.011642	2
CATGGGT	9570	0.0	27.43114	4
GTACCTG	3300	0.0	26.803118	1
ACATGGG	20645	0.0	26.047504	3
ACGCAGA	14130	0.0	25.243692	7
CGCAGAG	13910	0.0	25.237522	8
ACCTGGG	3565	0.0	23.622631	3
ATGGGTG	5200	0.0	23.161047	5
GCAGAGT	15235	0.0	22.20973	9
GTGGTAT	2550	0.0	22.140247	1
TGGTATC	2550	0.0	20.848415	2
>>END_MODULE