##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431390_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3296883 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.628001660962795 34.0 31.0 34.0 31.0 34.0 2 32.757603469701536 34.0 31.0 34.0 31.0 34.0 3 32.849668307913866 34.0 31.0 34.0 31.0 34.0 4 36.31495627839993 37.0 37.0 37.0 35.0 37.0 5 36.22922105516028 37.0 37.0 37.0 35.0 37.0 6 36.277467838561456 37.0 37.0 37.0 35.0 37.0 7 36.2315541679823 37.0 37.0 37.0 35.0 37.0 8 36.24955935651948 37.0 37.0 37.0 35.0 37.0 9 38.04876636507877 39.0 39.0 39.0 35.0 39.0 10-11 38.02475474561882 39.0 39.0 39.0 35.0 39.0 12-13 38.007877895575916 39.0 39.0 39.0 35.0 39.0 14-15 39.46249366447035 41.0 39.0 41.0 36.0 41.0 16-17 39.49272767641436 41.0 39.0 41.0 36.5 41.0 18-19 39.566988728444414 41.0 39.5 41.0 37.0 41.0 20-21 39.55711303676837 41.0 40.0 41.0 37.0 41.0 22-23 39.48236485795826 41.0 39.5 41.0 36.0 41.0 24-25 39.42482793596254 41.0 39.0 41.0 36.0 41.0 26-27 39.30129625467449 41.0 39.0 41.0 35.5 41.0 28-29 39.2169568953463 41.0 39.0 41.0 35.0 41.0 30-31 39.015320076569296 41.0 39.0 41.0 35.0 41.0 32-33 38.76291712505418 41.0 38.0 41.0 35.0 41.0 34-35 38.610858195453105 41.0 38.0 41.0 35.0 41.0 36-37 38.463849338905874 41.0 38.0 41.0 35.0 41.0 38-39 38.3183617677667 40.5 38.0 41.0 35.0 41.0 40-41 38.17155067377277 40.0 38.0 41.0 34.0 41.0 42-43 37.98928700229884 40.0 38.0 41.0 34.0 41.0 44-45 37.722009394934545 40.0 38.0 41.0 33.0 41.0 46-47 37.30832349828611 40.0 37.5 41.0 32.5 41.0 48-49 36.93617107431474 40.0 37.0 41.0 31.0 41.0 50-51 36.761562663885854 40.0 37.0 41.0 31.0 41.0 52-53 36.69763895170074 40.0 37.0 41.0 31.0 41.0 54-55 36.55288298068206 40.0 36.0 41.0 31.0 41.0 56-57 36.3992446805058 40.0 36.0 41.0 31.0 41.0 58-59 36.21657441286209 40.0 35.5 41.0 30.0 41.0 60-61 36.05366811621765 39.5 35.0 41.0 30.0 41.0 62-63 35.882439564885985 39.0 35.0 41.0 30.0 41.0 64-65 35.64154050962682 39.0 35.0 41.0 29.5 41.0 66-67 35.37186412135341 38.5 35.0 41.0 29.0 41.0 68-69 35.07197176848557 37.5 35.0 40.5 29.0 41.0 70-71 34.761573279973845 37.0 35.0 40.0 28.5 41.0 72-73 34.43513934222112 36.5 35.0 39.5 28.0 41.0 74-75 34.09672196435239 36.0 35.0 39.0 28.0 41.0 76-77 33.76175193356877 36.0 35.0 39.0 27.5 41.0 78-79 33.4386761677621 35.0 34.0 37.5 27.0 39.5 80-81 33.170799661377124 35.0 34.0 37.0 27.0 39.0 82-83 32.967206904218315 35.0 34.0 37.0 27.5 39.0 84-85 32.70465800575877 35.0 34.0 36.0 27.0 38.0 86-87 32.46338814571217 35.0 34.0 36.0 27.0 37.0 88-89 32.2346197908752 35.0 34.0 36.0 26.0 37.0 90-91 32.05502363899477 35.0 34.0 35.0 26.0 36.5 92-93 31.8975498978884 35.0 34.0 35.0 26.0 36.0 94-95 31.757431185759398 35.0 34.0 35.0 25.5 36.0 96-97 31.61895296254068 35.0 34.0 35.0 25.0 36.0 98-99 31.503025585075356 35.0 34.0 35.0 25.0 36.0 100 31.403079211485515 35.0 34.0 35.0 24.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05662351813916189 1101 2 -0.004867346025442032 1101 3 -0.01783423395735184 1101 4 0.009653473992358386 1101 5 -0.023007806966163002 1101 6 -0.01699236387357672 1101 7 -0.007645135360306199 1101 8 -0.014828573210785123 1101 9 0.013912610217445831 1101 10-11 0.030271228848462783 1101 12-13 -0.028111783792866163 1101 14-15 -0.03571354779004565 1101 16-17 -0.06088249653294042 1101 18-19 -0.09195788978133379 1101 20-21 -0.011514952930745892 1101 22-23 -0.03654922174457198 1101 24-25 -0.013852333559945862 1101 26-27 -0.030790968851135858 1101 28-29 -0.0806913468210837 1101 30-31 -0.15102136620699014 1101 32-33 -0.08288361110115972 1101 34-35 -0.022979618909147348 1101 36-37 -0.0988293294603011 1101 38-39 -0.14227406448754465 1101 40-41 -0.17205980081377703 1101 42-43 -0.1544246510330396 1101 44-45 -0.17932543803127743 1101 46-47 -0.16573115646459513 1101 48-49 -0.16497281218615711 1101 50-51 -0.2711024020975188 1101 52-53 -0.21606676686690207 1101 54-55 -0.19105974604166676 1101 56-57 -0.13313574885594903 1101 58-59 -0.13334883797802632 1101 60-61 -0.13682824801292526 1101 62-63 -0.205974157972733 1101 64-65 -0.267738015028705 1101 66-67 -0.19594312216448628 1101 68-69 -0.22250917847489404 1101 70-71 -0.2464513157717576 1101 72-73 -0.2589066458211704 1101 74-75 -0.24439168701627523 1101 76-77 -0.27509540768384255 1101 78-79 -0.25708794561604975 1101 80-81 -0.246006176575996 1101 82-83 -0.29115095537752467 1101 84-85 -0.19160978200959988 1101 86-87 -0.18923834796889594 1101 88-89 -0.18040717233764525 1101 90-91 -0.22520286344869334 1101 92-93 -0.2987734899093617 1101 94-95 -0.30895107136944233 1101 96-97 -0.30102805280763434 1101 98-99 -0.24995490262227094 1101 100 -0.22268987970112875 1102 1 -0.06770407765722553 1102 2 -0.02773864979979379 1102 3 -0.03790466436863227 1102 4 -0.013443945242840982 1102 5 -0.00777960262055899 1102 6 -0.05066534842448789 1102 7 -0.07365019558439911 1102 8 -0.036730114944830916 1102 9 0.010012456743943687 1102 10-11 -0.0156346291925189 1102 12-13 -0.05332983594211527 1102 14-15 -0.06120245144663983 1102 16-17 -0.06613848593194405 1102 18-19 -0.0630043326523193 1102 20-21 -0.023689200553420164 1102 22-23 -0.07813593174747524 1102 24-25 -0.0786237702436523 1102 26-27 -0.05477404455535151 1102 28-29 -0.06813621144765136 1102 30-31 0.0033176785485409255 1102 32-33 -0.006134413644183212 1102 34-35 -0.04770909209891272 1102 36-37 -0.011420776794068388 1102 38-39 -0.019445250937998537 1102 40-41 -0.0023863937958523707 1102 42-43 0.056731788396156446 1102 44-45 0.06502568906454798 1102 46-47 0.05028505169367037 1102 48-49 0.1230029502567831 1102 50-51 0.05820107930491503 1102 52-53 0.05631708536849089 1102 54-55 0.059277442889481335 1102 56-57 0.03520802348952401 1102 58-59 0.05134645542911187 1102 60-61 0.022780630865675278 1102 62-63 0.03187193307090297 1102 64-65 -0.008474406431588477 1102 66-67 -0.01699568918377281 1102 68-69 -0.01063182126405593 1102 70-71 -0.010036913506674239 1102 72-73 0.03345744318335875 1102 74-75 -0.0223817505631132 1102 76-77 0.010049672397293818 1102 78-79 0.031045926281564107 1102 80-81 0.026862691970016783 1102 82-83 -0.009340063665455034 1102 84-85 -0.028703198090191506 1102 86-87 -0.008517257015462576 1102 88-89 -0.09075455675809252 1102 90-91 -0.06872834892498858 1102 92-93 -0.0460290326694448 1102 94-95 -0.027984121566895226 1102 96-97 -7.458188626436879E-4 1102 98-99 0.00982037450671669 1102 100 0.014073301378122238 1103 1 -0.125072193798772 1103 2 -0.07822828493593192 1103 3 -0.061625332148068424 1103 4 -0.006728580350305435 1103 5 -0.028975734868652125 1103 6 -0.05212048754998477 1103 7 -0.0627733980806866 1103 8 -0.03105259872642563 1103 9 -0.03763286358024942 1103 10-11 -0.027314101370286892 1103 12-13 -0.003177187940231363 1103 14-15 -0.036038295692613076 1103 16-17 -0.04174509862330922 1103 18-19 -0.03427349072940444 1103 20-21 -0.0774943947440434 1103 22-23 -0.06559176492307017 1103 24-25 -0.06372607093368998 1103 26-27 -0.13106667701280372 1103 28-29 -0.15193881729028647 1103 30-31 -0.16381180768230763 1103 32-33 -0.14089503088990085 1103 34-35 -0.08605518075377461 1103 36-37 -0.029790519077785405 1103 38-39 -0.036853862895164013 1103 40-41 0.0110567041340488 1103 42-43 -0.02458231085874729 1103 44-45 0.005396891147846361 1103 46-47 -0.04210194289732527 1103 48-49 -0.03933361098309973 1103 50-51 -0.1030458304513644 1103 52-53 -0.01511038496229844 1103 54-55 -0.017566020133706672 1103 56-57 -0.017316261093959895 1103 58-59 0.02044168799324808 1103 60-61 0.06367276486896145 1103 62-63 0.010106866161024186 1103 64-65 0.0014032600687343688 1103 66-67 0.025841111532074024 1103 68-69 0.010354052522075108 1103 70-71 0.038626538159341806 1103 72-73 0.014830300088583215 1103 74-75 0.028855529965056803 1103 76-77 0.035816282278041456 1103 78-79 0.09079553767086423 1103 80-81 0.0678311858239482 1103 82-83 0.05677932155528964 1103 84-85 0.042168064220597046 1103 86-87 0.030928162540696746 1103 88-89 0.0482879480744387 1103 90-91 0.12093374618855535 1103 92-93 0.10098823684569425 1103 94-95 0.07209801008465888 1103 96-97 0.07661244578267556 1103 98-99 0.057080669105168624 1103 100 0.11031001548110453 1104 1 -0.10610923224282942 1104 2 -0.0815988937965173 1104 3 -0.07498773103596079 1104 4 -0.011451425179707542 1104 5 -0.02667833194964686 1104 6 -0.06461352279166022 1104 7 -0.1099995599641872 1104 8 -0.09759901778538449 1104 9 -0.10876758100101114 1104 10-11 -0.12110996148980746 1104 12-13 -0.10750091342463008 1104 14-15 -0.16156509347965198 1104 16-17 -0.07955349143849588 1104 18-19 -0.11215958281054128 1104 20-21 -0.07718005726140831 1104 22-23 -0.10556754304135296 1104 24-25 -0.12347762956218133 1104 26-27 -0.1601589210157286 1104 28-29 -0.14833552218573942 1104 30-31 -0.10976862477367888 1104 32-33 -0.03213070037201504 1104 34-35 -0.09586612449962928 1104 36-37 -0.13284774898937712 1104 38-39 -0.15895302346591222 1104 40-41 -0.15528269803642303 1104 42-43 -0.07144867086498863 1104 44-45 -0.09661034868325657 1104 46-47 -0.09712058769606102 1104 48-49 -0.09592838410785731 1104 50-51 -0.14955546644073792 1104 52-53 -0.07410087666016807 1104 54-55 -0.028312149768261463 1104 56-57 -0.06143391591209735 1104 58-59 -0.03780624790564957 1104 60-61 -0.08096411619970212 1104 62-63 -0.11688591652021785 1104 64-65 -0.09340037947476532 1104 66-67 -0.05102185579266205 1104 68-69 -0.11288228951650581 1104 70-71 -0.13440118650336075 1104 72-73 -0.12187453908178014 1104 74-75 -0.14368734375051417 1104 76-77 -0.09197832699901909 1104 78-79 -0.1567946735371919 1104 80-81 -0.11291849478208604 1104 82-83 -0.09182417404987575 1104 84-85 -0.1316962228906604 1104 86-87 -0.13368882499731427 1104 88-89 -0.034923194202200136 1104 90-91 -0.057039924210940285 1104 92-93 -0.05631817277214424 1104 94-95 -0.021413147950632094 1104 96-97 -0.049404918977899115 1104 98-99 -0.061592217301736696 1104 100 0.006329427458521053 1105 1 -0.13347085704818085 1105 2 -0.117404248552198 1105 3 -0.11263606133044135 1105 4 -0.0319057249304322 1105 5 -0.07558297643858225 1105 6 -0.04621252735097414 1105 7 -0.02686078940688219 1105 8 -0.032890002616071 1105 9 0.008396459501312847 1105 10-11 -0.028309754418174293 1105 12-13 -0.05533747237662823 1105 14-15 -0.06263039831083717 1105 16-17 -0.07029395786246795 1105 18-19 -0.1569256968704167 1105 20-21 -0.045349249259352575 1105 22-23 -0.12308209107010981 1105 24-25 -0.1083796967440307 1105 26-27 -0.029740919437038826 1105 28-29 -0.05851703730134261 1105 30-31 -0.06900490783809232 1105 32-33 -0.12699200812493672 1105 34-35 -0.13674397164778895 1105 36-37 -0.13368707166836913 1105 38-39 -0.10523983706472251 1105 40-41 -0.15446511857061296 1105 42-43 -0.24991869710930814 1105 44-45 -0.2996152953941973 1105 46-47 -0.35941845377727333 1105 48-49 -0.3424754145913269 1105 50-51 -0.31082951304848194 1105 52-53 -0.3381621086670137 1105 54-55 -0.2713036451399873 1105 56-57 -0.2751605623151292 1105 58-59 -0.35549585911967085 1105 60-61 -0.35412618231431026 1105 62-63 -0.38037758029356894 1105 64-65 -0.3602417974718506 1105 66-67 -0.3945286306660236 1105 68-69 -0.30194022105951035 1105 70-71 -0.301126715773016 1105 72-73 -0.2676933191887656 1105 74-75 -0.26709056029073963 1105 76-77 -0.3220817276722059 1105 78-79 -0.2008931150252522 1105 80-81 -0.2378578563389766 1105 82-83 -0.30372560331530707 1105 84-85 -0.33650988442003893 1105 86-87 -0.3704393684910201 1105 88-89 -0.2879773793289466 1105 90-91 -0.3083371970749269 1105 92-93 -0.2910682107144851 1105 94-95 -0.30755517600509563 1105 96-97 -0.35862299318410606 1105 98-99 -0.3303006063358076 1105 100 -0.28466900356507807 1106 1 -0.07526763026381644 1106 2 -0.05366205165001503 1106 3 -0.030215391768798838 1106 4 0.01521157728885214 1106 5 0.010165722157971402 1106 6 -0.0526342094438732 1106 7 -0.016811349087255678 1106 8 -0.03740085302403884 1106 9 -0.04335019071341151 1106 10-11 -0.050969281320050186 1106 12-13 -0.049913958196974306 1106 14-15 -0.063924894682998 1106 16-17 -0.0593603903559341 1106 18-19 -0.05450113484256747 1106 20-21 -0.03657335172778886 1106 22-23 -0.09754630454522584 1106 24-25 -0.019779240699413947 1106 26-27 -0.10669872348115206 1106 28-29 -0.05728992191029647 1106 30-31 -0.13564763419003611 1106 32-33 -0.052919609774320975 1106 34-35 -0.052298197326678064 1106 36-37 -0.057029186919166364 1106 38-39 -0.1536217472272412 1106 40-41 -0.22260744139707356 1106 42-43 -0.1681159892979167 1106 44-45 -0.1759588594839414 1106 46-47 -0.17558161342228829 1106 48-49 -0.15777605543303963 1106 50-51 -0.19281700527960055 1106 52-53 -0.10719322254264796 1106 54-55 -0.2023069145750327 1106 56-57 -0.21375711913432127 1106 58-59 -0.22832997246707265 1106 60-61 -0.17696172777159092 1106 62-63 -0.28780521607701814 1106 64-65 -0.23231258357935047 1106 66-67 -0.21131262473007695 1106 68-69 -0.1925368692971574 1106 70-71 -0.17604072581249142 1106 72-73 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28-29 -0.22715876002040147 1107 30-31 -0.2567322806383885 1107 32-33 -0.2145171241521311 1107 34-35 -0.2095707921063621 1107 36-37 -0.28027403343464385 1107 38-39 -0.2145347494137937 1107 40-41 -0.33607310103873544 1107 42-43 -0.3432304530127368 1107 44-45 -0.3387218565187169 1107 46-47 -0.36746867823990215 1107 48-49 -0.32081048980865745 1107 50-51 -0.30789296233308505 1107 52-53 -0.2910695998648052 1107 54-55 -0.25365152763325227 1107 56-57 -0.33695587955733686 1107 58-59 -0.3436823216864582 1107 60-61 -0.3778916800207597 1107 62-63 -0.3212458694007694 1107 64-65 -0.2803005618054826 1107 66-67 -0.2951102638699652 1107 68-69 -0.3031483631230145 1107 70-71 -0.3738595040189736 1107 72-73 -0.39343016142721865 1107 74-75 -0.3409236667152129 1107 76-77 -0.3104578784544074 1107 78-79 -0.2764540607206456 1107 80-81 -0.2869146662197224 1107 82-83 -0.27441726622827645 1107 84-85 -0.28830982419219 1107 86-87 -0.24146532129734766 1107 88-89 -0.2637640529462786 1107 90-91 -0.28794043132753444 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2214 64-65 0.0625380824711641 2214 66-67 0.03535437799956043 2214 68-69 0.0573928086277391 2214 70-71 0.04054389245344936 2214 72-73 0.0638776989599279 2214 74-75 0.05821396170024684 2214 76-77 0.06574585974282598 2214 78-79 0.047391722524096735 2214 80-81 0.027715830391684904 2214 82-83 -0.025826075134609994 2214 84-85 0.045667258036694136 2214 86-87 0.03645733928465944 2214 88-89 0.020721497180524295 2214 90-91 0.015658873043967958 2214 92-93 0.0460485820820935 2214 94-95 0.020320848800455593 2214 96-97 0.012473402770392994 2214 98-99 -0.02388301705217799 2214 100 -0.004790520394077902 2215 1 0.11207455215260609 2215 2 0.06867595454542652 2215 3 0.07436189660908354 2215 4 0.011651233209455825 2215 5 0.060635566664245744 2215 6 0.05358859556912421 2215 7 0.04624199146169161 2215 8 0.013400609447863587 2215 9 0.022802791167741532 2215 10-11 0.06616709015074917 2215 12-13 0.025469930199179203 2215 14-15 0.012118021117295541 2215 16-17 -0.019835719917658423 2215 18-19 0.008190080284940393 2215 20-21 0.017290049960060117 2215 22-23 -0.05578266276178567 2215 24-25 0.010012276551442767 2215 26-27 0.00558827222248226 2215 28-29 0.02479759135486148 2215 30-31 -0.04270852983096063 2215 32-33 -0.07703389381218528 2215 34-35 -0.10026686702522625 2215 36-37 -0.13497550951341708 2215 38-39 -0.095085169102056 2215 40-41 -0.09755366043711433 2215 42-43 -0.03894038387683452 2215 44-45 -0.07153643093329265 2215 46-47 -0.08620562972273405 2215 48-49 -0.05485444702129172 2215 50-51 -0.02116424683456586 2215 52-53 -0.143401922877878 2215 54-55 -0.11483779529751104 2215 56-57 -0.09799726781930929 2215 58-59 -0.09739396285975488 2215 60-61 -0.09242067813000432 2215 62-63 -0.035680809221766197 2215 64-65 -0.1324609821824012 2215 66-67 -0.16296403504941992 2215 68-69 -0.11825016661877896 2215 70-71 -0.1255519865911694 2215 72-73 -0.07322462794063256 2215 74-75 -0.047508095579161136 2215 76-77 -0.15466143837320345 2215 78-79 -0.10427221353441496 2215 80-81 -0.06360241972598146 2215 82-83 -0.05902936514028312 2215 84-85 -0.06492644557314264 2215 86-87 -0.0856857053585216 2215 88-89 -0.11332632014329391 2215 90-91 -0.09973459099649418 2215 92-93 -0.14498212443636405 2215 94-95 -0.1528759692113013 2215 96-97 -0.16456399298373725 2215 98-99 -0.1970970333694524 2215 100 -0.15931111008475085 2216 1 0.1453529952207262 2216 2 0.08160776836097483 2216 3 0.06849631732411154 2216 4 0.05070069460565918 2216 5 0.04420341726957133 2216 6 0.018130509969701336 2216 7 0.074014109258421 2216 8 0.04043406506450964 2216 9 0.031283614366472534 2216 10-11 0.044169566866045784 2216 12-13 0.03850220983347441 2216 14-15 0.02910865123813977 2216 16-17 0.033902016058497964 2216 18-19 0.006876068324956464 2216 20-21 0.0024498430071275834 2216 22-23 -0.050945000721149825 2216 24-25 -0.02231777085101072 2216 26-27 0.0011305173507665245 2216 28-29 -0.008919807500817 2216 30-31 -0.06261448450989349 2216 32-33 0.023319399894816684 2216 34-35 0.08255148195875961 2216 36-37 0.03408743603682751 2216 38-39 -0.03385755940954027 2216 40-41 -0.05406852502587611 2216 42-43 0.010979041094955733 2216 44-45 0.04006818894192321 2216 46-47 0.0011759576981873465 2216 48-49 0.0015845414786426204 2216 50-51 0.09093280525325298 2216 52-53 0.048272180310831914 2216 54-55 0.10509894505795359 2216 56-57 0.06890965464707932 2216 58-59 0.06848102262065936 2216 60-61 0.0791212143698985 2216 62-63 0.04401429753286834 2216 64-65 0.05737594660191547 2216 66-67 0.02119949968405166 2216 68-69 0.03264809316576134 2216 70-71 0.0055024443146081126 2216 72-73 -0.016994471949708156 2216 74-75 -0.04021110015584384 2216 76-77 0.0024330729606703017 2216 78-79 0.05016308622880672 2216 80-81 0.0428621149251569 2216 82-83 0.025048909486805826 2216 84-85 0.08134392723987816 2216 86-87 0.055761310149641474 2216 88-89 0.07263094806246073 2216 90-91 0.07350363247730485 2216 92-93 0.01711607812922722 2216 94-95 0.04289322498574677 2216 96-97 0.09943332959110407 2216 98-99 0.14583520737038214 2216 100 0.17543711686265695 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45.0 3 334.0 4 76.0 5 135.0 6 304.0 7 828.0 8 1411.0 9 2408.0 10 3372.0 11 4771.0 12 7449.0 13 11127.0 14 11707.0 15 15096.0 16 19916.0 17 31282.0 18 37444.0 19 18882.0 20 11226.0 21 9878.0 22 9454.0 23 9471.0 24 10084.0 25 11907.0 26 14284.0 27 17868.0 28 22322.0 29 27880.0 30 35161.0 31 44022.0 32 56053.0 33 72332.0 34 101831.0 35 171226.0 36 269789.0 37 564758.0 38 1030521.0 39 634272.0 40 5957.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71050248809793 20.80231868583536 14.417742617985589 22.06943620808112 2 14.69762732490825 22.074817478948162 40.634802288197776 22.592752907945812 3 16.49696422250458 29.268795750123832 31.824933444024882 22.409306583346705 4 10.852614423987749 18.689622895322643 39.923831085300876 30.533931595388736 5 10.857487230046692 39.436328662320655 35.53052696293747 14.175657144695187 6 26.538460721839385 40.33794951170545 18.911195817382662 14.212393949072503 7 23.87937333535949 35.49704372281334 23.76608451073332 16.857498431093852 8 25.063795105862113 34.58120897829859 21.372520650566003 18.98247526527329 9 26.225589443119457 15.554631450372975 22.37046325271476 35.84931585379281 10-11 22.626841777521374 27.54165070462009 29.766919238565638 20.0645882792929 12-13 24.50495278494153 26.22874791564455 29.784622290906636 19.48167700850728 14-15 21.802108233746846 26.863767989340236 29.091584384401877 22.24253939251105 16-17 18.964367252341074 30.211005364764233 29.53143924124696 21.293188141647732 18-19 18.90686329300176 27.525604135668292 34.09906246194318 19.46847010938677 20-21 20.63355306111152 26.556239113768843 33.951437456445596 18.858770368674044 22-23 20.085614225804864 26.597873384610065 34.200587100175255 19.115925289409812 24-25 19.56902006198584 26.847609347255986 34.128033699719914 19.45533689103826 26-27 19.322392069828506 26.87641971477856 34.17303708934243 19.628151126050504 28-29 19.3481378443485 26.730394633953697 34.43726155471934 19.484205966978458 30-31 19.648339342312855 27.18145065708506 33.891569628964916 19.27864037163717 32-33 19.291086969445857 26.957725528355063 34.23738699771538 19.51380050448369 34-35 19.597728317456824 26.784934049956448 34.0216415571038 19.595696075482927 36-37 19.833437801850952 26.994159418705117 33.400918119785075 19.77148465965886 38-39 19.703301276432082 27.150722235736822 33.547548171798134 19.598428316032958 40-41 19.610464750358485 26.938717013295154 33.52667789446226 19.9241403418841 42-43 19.52116632012533 27.144772382247982 33.260112258160405 20.073949039466278 44-45 19.923594312796208 27.186274881516585 32.880758293838866 20.00937251184834 46-47 20.083545769320075 27.551980655623787 32.13283304146608 20.231640533590063 48-49 20.22260763747025 27.569499863052215 31.601012109338626 20.606880390138908 50-51 20.307840046686643 27.651644004040797 31.29062085924108 20.749895090031483 52-53 20.35087032839439 27.860472649876744 31.116693191913882 20.671963829814985 54-55 20.370829562690343 28.224595496642777 31.033487556233574 20.371087384433302 56-57 20.309884309412947 27.819353308394078 30.96766845293831 20.903093929254666 58-59 20.23880568946646 27.4736799161749 31.353062887479904 20.93445150687873 60-61 20.42140662291871 27.788568415295924 31.073714689477605 20.716310272307766 62-63 20.20649126369699 27.690991982793257 31.257475894009623 20.84504085950013 64-65 20.223891100106588 27.55392610953687 31.39179926389806 20.83038352645848 66-67 20.20762713985061 27.57891837761951 31.23919145771984 20.974263024810035 68-69 20.672677214243446 27.749402387900528 30.9096261273189 20.668294270537125 70-71 20.585979591264145 27.56677730182165 30.95360830605369 20.893634800860514 72-73 20.398647143333136 27.678737034504337 30.960905257113897 20.961710565048637 74-75 20.45677760872783 27.792160167141056 30.784647555125243 20.966414669005864 76-77 20.45705714768859 27.79479822042387 30.674347730145644 21.07379690174189 78-79 20.552827615325597 27.818731847258466 30.75096975128232 20.877470786133614 80-81 20.38168473791857 27.78139907190394 30.78736809027507 21.04954809990242 82-83 20.31238838694981 27.79479835981613 30.927872888631807 20.964940364602253 84-85 20.274793855318812 27.76981559586694 30.67173516418019 21.283655384634052 86-87 20.403127801468113 27.775488535090098 30.564524213556666 21.256859449885123 88-89 20.24231374180409 27.67495798630295 30.691420864484993 21.391307407407968 90-91 20.105060923324057 27.649643644583055 30.6531175677139 21.592177864378986 92-93 20.141092704088265 27.799578960395248 30.587476278417558 21.47185205709893 94-95 20.038915905397648 27.532457093546043 30.775550316551925 21.653076684504388 96-97 20.006742741656993 27.724770567068212 30.581836728287076 21.686649962987715 98-99 19.899471133780466 27.707497185203877 30.51445252215439 21.87857915886127 100 19.957474817023417 27.670790780346206 30.465350468777963 21.90638393385241 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5247.0 1 3005.5 2 3789.5 3 6164.0 4 3456.5 5 1803.5 6 3857.0 7 9300.5 8 16187.0 9 18002.5 10 11513.0 11 5230.5 12 4256.5 13 4573.5 14 4947.0 15 5264.0 16 5666.0 17 6156.0 18 6802.5 19 7516.5 20 8515.5 21 9965.0 22 12458.0 23 16405.0 24 20698.0 25 25600.5 26 32279.5 27 40538.0 28 51524.0 29 62007.5 30 68924.0 31 76399.5 32 84499.0 33 92223.0 34 98998.0 35 104993.0 36 109163.5 37 111509.0 38 113637.5 39 113839.5 40 114244.0 41 114273.0 42 112510.0 43 111565.5 44 110067.5 45 106756.5 46 103816.0 47 101384.0 48 98344.0 49 96319.5 50 95669.5 51 90674.0 52 80745.0 53 72774.0 54 68247.0 55 64698.0 56 58697.5 57 51607.0 58 47076.0 59 42986.0 60 37065.0 61 30294.5 62 24762.0 63 19573.5 64 14666.5 65 11284.0 66 8742.0 67 6447.5 68 4633.5 69 3405.0 70 2490.5 71 1793.5 72 1339.0 73 1016.0 74 846.0 75 656.5 76 400.0 77 292.0 78 219.0 79 113.0 80 48.5 81 18.5 82 7.0 83 5.5 84 5.0 85 4.0 86 2.0 87 1.0 88 1.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005702355831250306 2 0.00424643519348427 3 0.0010312771184176083 4 0.0 5 0.044041599292422566 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.5165839976729534E-5 14-15 0.0 16-17 0.0 18-19 1.0616087983710674E-4 20-21 1.5165839976729534E-5 22-23 3.0331679953459068E-5 24-25 3.0331679953459068E-5 26-27 1.8199007972075442E-4 28-29 1.5165839976729535E-4 30-31 3.488143194647793E-4 32-33 0.0014255889578125764 34-35 9.554479185339607E-4 36-37 0.001107106318301256 38-39 0.0011222721582779857 40-41 1.6682423974402489E-4 42-43 5.459702391622633E-4 44-45 2.4265343962767255E-4 46-47 2.2748759965094304E-4 48-49 1.8199007972075442E-4 50-51 2.578192796044021E-4 52-53 7.279603188830177E-4 54-55 9.554479185339607E-4 56-57 0.0021838809566490534 58-59 5.611360791389928E-4 60-61 0.0013194280779754697 62-63 0.001107106318301256 64-65 0.001728905757347167 66-67 3.488143194647793E-4 68-69 3.6398015944150885E-4 70-71 3.1848263951132023E-4 72-73 2.2748759965094304E-4 74-75 8.189553587433949E-4 76-77 9.251162385805016E-4 78-79 9.857795984874198E-4 80-81 7.88623678789936E-4 82-83 0.0017440715973238963 84-85 0.0014559206377660354 86-87 0.0018957299970911918 88-89 0.0012587647180685513 90-91 0.0010312771184176083 92-93 0.0013194280779754697 94-95 9.251162385805016E-4 96-97 1.3649255979056584E-4 98-99 5.763019191157223E-4 100 3.94311839394968E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3296883.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.19486973225339 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53694680629822 43.958627793646784 2 15.266771734048667 17.76895584590021 3 4.561998406229193 7.964547089077664 4 1.7961085813646198 4.18097219669986 5 0.8441457721679408 2.456247662317288 6 0.4707566768872755 1.6437374092226096 7 0.29836317974162485 1.2154244464575334 8 0.19256195196718517 0.896489416809502 9 0.1584436598008176 0.8298547327809047 >10 0.7633554635964968 8.1696798811318 >50 0.06831871049768838 2.7380432472211678 >100 0.03972454399805912 4.059501719011654 >500 0.001513143514943245 0.5884042144990397 >1k 6.261283498087251E-4 0.5890418691219701 >5k 2.0870944993624165E-4 0.794063298077666 >10k+ 1.5653208745218126E-4 2.146409178024322 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30411 0.9224167190646438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24451 0.7416399065420277 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15826 0.4800291669434432 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8662 0.2627330117568625 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6970 0.21141180927560974 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5316 0.1612432106325884 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5203 0.15781573079784753 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4716 0.143044202660513 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 3.033167995345907E-5 0.0 0.0 3.033167995345907E-5 0.0 10-11 3.033167995345907E-5 0.0 0.0 3.033167995345907E-5 0.0 12-13 3.033167995345907E-5 0.0 0.0 3.033167995345907E-5 0.0 14-15 6.066335990691814E-5 0.0 0.0 3.033167995345907E-5 0.0 16-17 6.066335990691814E-5 0.0 0.0 6.066335990691814E-5 0.0 18-19 7.582919988364767E-5 0.0 0.0 9.099503986037721E-5 0.0 20-21 9.099503986037721E-5 0.0 0.0 1.8199007972075442E-4 0.0 22-23 9.099503986037721E-5 0.0 0.0 5.914677590924519E-4 0.0 24-25 9.099503986037721E-5 0.0 0.0 0.0011981013581616332 0.0 26-27 9.099503986037721E-5 0.0 0.0 0.0019867250369515694 0.0 28-29 9.099503986037721E-5 0.0 0.0 0.004018947593833326 0.0 30-31 9.099503986037721E-5 0.0 0.0 0.008250216947340867 0.0 32-33 9.099503986037721E-5 0.0 0.0 0.0168947457340767 0.0 34-35 9.099503986037721E-5 0.0 0.0 0.030149689873738318 0.0 36-37 9.099503986037721E-5 0.0 0.0 0.051670016800717526 0.0 38-39 9.099503986037721E-5 0.0 0.0 0.08036378603668981 0.0 40-41 9.099503986037721E-5 0.0 0.0 0.11366797062558787 0.0 42-43 9.099503986037721E-5 0.0 0.0 0.1468053309747419 0.0 44-45 9.099503986037721E-5 0.0 0.0 0.18622134907426197 0.0 46-47 9.099503986037721E-5 0.0 0.0 0.23422123260061095 0.0 48-49 9.099503986037721E-5 0.0 0.0 0.28846944219737247 0.0 50-51 9.099503986037721E-5 0.0 0.0 0.3457204881095265 0.0 52-53 9.099503986037721E-5 0.0 0.0 0.4010606381846126 0.0 54-55 9.099503986037721E-5 0.0 0.0 0.46402920576799356 0.0 56-57 9.099503986037721E-5 0.0 0.0 0.5415569797290349 0.0 58-59 9.099503986037721E-5 0.0 0.0 0.6309596063918557 0.0 60-61 9.099503986037721E-5 0.0 0.0 0.7269745392845303 0.0 62-63 9.099503986037721E-5 1.5165839976729536E-5 0.0 0.8224131702580892 0.0 64-65 9.099503986037721E-5 3.033167995345907E-5 0.0 0.9246915950611532 0.0 66-67 9.099503986037721E-5 3.033167995345907E-5 0.0 1.047625893912523 0.0 68-69 9.099503986037721E-5 3.033167995345907E-5 0.0 1.1873336117781554 0.0 70-71 9.099503986037721E-5 3.033167995345907E-5 0.0 1.346271614734281 0.0 72-73 9.099503986037721E-5 3.033167995345907E-5 0.0 1.5184493959900913 0.0 74-75 9.099503986037721E-5 3.033167995345907E-5 0.0 1.6902176995665301 0.0 76-77 9.099503986037721E-5 3.033167995345907E-5 0.0 1.8768181946402103 0.0 78-79 9.099503986037721E-5 3.033167995345907E-5 0.0 2.0931437360682805 0.0 80-81 9.099503986037721E-5 3.033167995345907E-5 0.0 2.3388000120113457 0.0 82-83 9.099503986037721E-5 3.033167995345907E-5 0.0 2.601411697048394 0.0 84-85 9.099503986037721E-5 3.033167995345907E-5 0.0 2.8619456620086305 0.0 86-87 9.099503986037721E-5 3.033167995345907E-5 0.0 3.1394047043828976 0.0 88 9.099503986037721E-5 3.033167995345907E-5 0.0 3.3664524946745153 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10060 0.0 64.17557 1 GTATCAA 20350 0.0 45.24238 1 TATAACG 325 0.0 43.403214 2 GTGGTAT 3265 0.0 37.300426 1 ATCAACG 26375 0.0 34.709175 3 TCAACGC 26620 0.0 34.3544 4 TATCAAC 26750 0.0 34.27637 2 TGGTATC 3415 0.0 33.871033 2 ATAACGC 480 0.0 33.30493 3 CAACGCA 27475 0.0 33.233982 5 AACGCAG 28395 0.0 32.258915 6 TACCTGG 2745 0.0 32.031944 2 CGCAGAG 32005 0.0 28.473429 8 ACGCAGA 32090 0.0 28.441946 7 CATGGGT 9560 0.0 27.984995 4 GTACCTG 3190 0.0 27.41697 1 GTACATG 19260 0.0 27.392632 1 TACATGG 19045 0.0 27.059116 2 ACATGGG 19100 0.0 26.709589 3 GCAGAGT 34385 0.0 26.31125 9 >>END_MODULE