FastQCFastQC Report
Wed 25 May 2016
SRR2431389_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431389_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4521451
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151500.3350694279336434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60800.047.2844621
GTATCAA105150.037.7223851
TCAACGC118150.032.3333134
ATCAACG119750.031.9812953
TATCAAC128750.030.440022
CAACGCA125750.030.3410875
AACGCAG134250.028.459876
GTACATG228450.028.0315951
TACATGG228200.027.2354352
CATGGGT104300.026.5680034
ACATGGG231750.025.8234673
CGCAGAG150250.024.8977478
ACGCAGA152850.024.7509527
ATGGGTG57450.021.8644985
GCAGAGT169050.021.2392739
ATGGGTA48550.021.1244565
GTGGTAT30250.020.6874581
TACCTGG35450.020.4386232
GTACCTG38200.020.3236371
CATGGGG100350.018.7061084
Produced by FastQC (version 0.11.5)