##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431389_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4521451
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92450609328731	33.0	31.0	34.0	27.0	34.0
2	31.060154140783567	33.0	31.0	34.0	27.0	34.0
3	31.10461508927112	34.0	31.0	34.0	27.0	34.0
4	34.3138567685462	37.0	35.0	37.0	30.0	37.0
5	34.41501080073631	37.0	35.0	37.0	31.0	37.0
6	34.39769423576635	37.0	35.0	37.0	31.0	37.0
7	34.36111792431235	37.0	35.0	37.0	31.0	37.0
8	34.33532576157521	37.0	35.0	37.0	31.0	37.0
9	35.7954983919985	39.0	37.0	39.0	30.0	39.0
10-11	35.818377994143916	39.0	37.0	39.0	30.5	39.0
12-13	35.758466142837776	39.0	37.0	39.0	30.0	39.0
14-15	37.123602467438	41.0	38.0	41.0	30.5	41.0
16-17	37.14640819949172	41.0	38.0	41.0	31.0	41.0
18-19	37.17268084957683	41.0	38.0	41.0	30.5	41.0
20-21	37.26102560881452	41.0	38.0	41.0	31.0	41.0
22-23	37.22630898797753	40.5	38.0	41.0	30.0	41.0
24-25	37.269054447344445	41.0	38.0	41.0	30.0	41.0
26-27	37.312549113105504	41.0	38.0	41.0	30.0	41.0
28-29	37.279520888316604	40.0	38.0	41.0	30.0	41.0
30-31	37.214089348751095	40.0	38.0	41.0	30.0	41.0
32-33	37.16977735687061	40.0	38.0	41.0	30.0	41.0
34-35	37.09851052239646	40.0	38.0	41.0	30.0	41.0
36-37	36.9654876277549	40.0	38.0	41.0	30.0	41.0
38-39	36.844453694179144	40.0	38.0	41.0	30.0	41.0
40-41	36.788398237645396	40.0	37.0	41.0	30.0	41.0
42-43	36.619054701687574	40.0	37.0	41.0	29.0	41.0
44-45	36.461823096169795	40.0	37.0	41.0	29.0	41.0
46-47	36.334199353260715	40.0	36.5	41.0	29.0	41.0
48-49	36.202743765220504	40.0	36.0	41.0	28.5	41.0
50-51	36.07367568508428	40.0	36.0	41.0	28.0	41.0
52-53	35.91968374754033	40.0	35.5	41.0	28.0	41.0
54-55	35.766959765791995	40.0	35.0	41.0	28.0	41.0
56-57	35.58281345966151	39.0	35.0	41.0	28.0	41.0
58-59	35.377550259861266	39.0	35.0	41.0	27.5	41.0
60-61	35.14871055773909	39.0	35.0	41.0	27.0	41.0
62-63	35.054392494798684	39.0	35.0	41.0	27.0	41.0
64-65	34.80616432645184	38.0	34.0	40.5	26.0	41.0
66-67	34.54095952825763	38.0	34.0	40.0	26.0	41.0
68-69	34.24013662870614	37.0	34.0	40.0	26.0	41.0
70-71	33.89817947822502	37.0	34.0	40.0	25.0	41.0
72-73	33.54716572180037	36.0	33.5	39.0	24.5	41.0
74-75	33.25429812243901	36.0	33.0	39.0	24.0	41.0
76-77	32.97781265350437	35.5	33.0	39.0	24.0	40.5
78-79	32.62204644040155	35.0	33.0	37.5	23.0	39.5
80-81	32.28754121188088	35.0	33.0	37.0	22.5	39.0
82-83	31.950557354265257	35.0	33.0	37.0	21.5	39.0
84-85	31.642359499196168	35.0	33.0	36.0	20.5	38.0
86-87	31.353272212836103	35.0	32.0	36.0	20.0	37.0
88-89	31.077364987478575	35.0	32.0	36.0	18.5	37.0
90-91	30.841507516060663	35.0	32.0	35.0	17.5	36.5
92-93	30.60646515908278	35.0	32.0	35.0	11.5	36.0
94-95	30.391068154890988	35.0	32.0	35.0	4.5	36.0
96-97	30.189185728209814	35.0	32.0	35.0	2.0	36.0
98-99	29.97329640418529	35.0	31.0	35.0	2.0	35.5
100	29.801721173136677	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.34319365507818134
1101	2	0.3703666803894414
1101	3	0.4081242210123257
1101	4	0.4520826598555061
1101	5	0.4189206402190564
1101	6	0.48801604311280755
1101	7	0.4544401185889768
1101	8	0.4012305934951286
1101	9	0.4002083226611646
1101	10-11	0.40321186592193214
1101	12-13	0.3486918784130282
1101	14-15	0.4240491379337996
1101	16-17	0.4406109801679321
1101	18-19	0.3908808815504017
1101	20-21	0.32896302553067613
1101	22-23	0.34036833865457083
1101	24-25	0.394804096643405
1101	26-27	0.42282246602233187
1101	28-29	0.4380165196416499
1101	30-31	0.4462450066957686
1101	32-33	0.36926824397605174
1101	34-35	0.38112819756374705
1101	36-37	0.3992907751648289
1101	38-39	0.47896874706874826
1101	40-41	0.37347629846504304
1101	42-43	0.35414753652944597
1101	44-45	0.4130870203730055
1101	46-47	0.3800401212293494
1101	48-49	0.4078080180081258
1101	50-51	0.43635213503552706
1101	52-53	0.3839152328818898
1101	54-55	0.43703349208696807
1101	56-57	0.4249313301248421
1101	58-59	0.4254354903095603
1101	60-61	0.370918189720129
1101	62-63	0.37858312659862037
1101	64-65	0.44389645436099556
1101	66-67	0.4231739236498129
1101	68-69	0.4447185031669605
1101	70-71	0.4498436789976452
1101	72-73	0.4415477211437491
1101	74-75	0.4009490229294599
1101	76-77	0.45392922866305696
1101	78-79	0.43322413368535706
1101	80-81	0.3798854072142035
1101	82-83	0.37391803602383433
1101	84-85	0.34548423632096714
1101	86-87	0.37784295035003623
1101	88-89	0.2866305290879225
1101	90-91	0.3245980984514958
1101	92-93	0.33699323788469826
1101	94-95	0.24073435014984668
1101	96-97	0.2961758762434954
1101	98-99	0.2324062482978171
1101	100	0.22732291931221837
1102	1	0.07232099357423749
1102	2	0.15436711998831854
1102	3	0.17761611583672732
1102	4	0.2010016644985413
1102	5	0.18267997940862557
1102	6	0.1804412465062839
1102	7	0.09174196501117393
1102	8	0.16380058774998218
1102	9	0.1265211972802902
1102	10-11	0.18537797086703733
1102	12-13	0.12308883814869631
1102	14-15	0.15809846817862905
1102	16-17	0.14086848666207885
1102	18-19	0.11104557243345425
1102	20-21	0.1067389502651892
1102	22-23	0.13779389852130208
1102	24-25	0.18152564712556796
1102	26-27	0.11291492551652738
1102	28-29	0.12835753638130853
1102	30-31	0.15524161964152228
1102	32-33	0.16683842436673046
1102	34-35	0.16064017438817046
1102	36-37	0.11266952658855445
1102	38-39	0.06780177620602501
1102	40-41	0.09854522276559408
1102	42-43	0.0734511087050933
1102	44-45	-4.789431419283119E-5
1102	46-47	-0.06998162708418221
1102	48-49	-0.07017723409568077
1102	50-51	-0.06989990227269516
1102	52-53	-0.04558458394003395
1102	54-55	-0.11371062475605243
1102	56-57	-0.08437165976759786
1102	58-59	-0.0939983721887856
1102	60-61	-0.06692785274605484
1102	62-63	-0.043790170914917326
1102	64-65	-0.05579122469969633
1102	66-67	-0.04104364463049137
1102	68-69	-0.15461068566881409
1102	70-71	-0.14295967350921757
1102	72-73	-0.1338889930456375
1102	74-75	-0.07639416156197854
1102	76-77	-0.11243072133233767
1102	78-79	-0.07019686926291513
1102	80-81	-0.0700821989762801
1102	82-83	0.0019461492558967564
1102	84-85	-0.08190426339320211
1102	86-87	-0.0971388749778761
1102	88-89	-0.12454121692474729
1102	90-91	-0.10815459327796262
1102	92-93	-0.14956151969816034
1102	94-95	-0.14851797278836543
1102	96-97	-0.14912042169203232
1102	98-99	-0.22104623144111812
1102	100	-0.254885477781837
1103	1	-0.23076862181947178
1103	2	-0.1419267168983822
1103	3	-0.13736878028425892
1103	4	-0.062193032154773675
1103	5	-0.08021696153952007
1103	6	-0.13042275363507372
1103	7	-0.11318525914872879
1103	8	-0.12273701005632631
1103	9	-0.14423675693982574
1103	10-11	-0.1732200295613282
1103	12-13	-0.09458666400662707
1103	14-15	-0.12166719306706142
1103	16-17	-0.15693109390114302
1103	18-19	-0.15288017775213802
1103	20-21	-0.2444078628772317
1103	22-23	-0.24799513141353202
1103	24-25	-0.1754907141564459
1103	26-27	-0.14603401833419838
1103	28-29	-0.15317007693837326
1103	30-31	-0.15972435335224588
1103	32-33	-0.18145864938954048
1103	34-35	-0.21750311224742802
1103	36-37	-0.32397734780032295
1103	38-39	-0.23987253683669962
1103	40-41	-0.2173773172935327
1103	42-43	-0.30619149848875793
1103	44-45	-0.49530427821322576
1103	46-47	-0.5540731987830227
1103	48-49	-0.5730349964102146
1103	50-51	-0.6483823247768186
1103	52-53	-0.726079388496963
1103	54-55	-0.7141395410136369
1103	56-57	-0.6852404999315169
1103	58-59	-0.6139006787090651
1103	60-61	-0.6545404871390588
1103	62-63	-0.5819913205336888
1103	64-65	-0.5665371612416834
1103	66-67	-0.5477751561382576
1103	68-69	-0.5333390610711817
1103	70-71	-0.49624564768732427
1103	72-73	-0.529116317707043
1103	74-75	-0.5012780380308541
1103	76-77	-0.46848390140863216
1103	78-79	-0.5165634497547344
1103	80-81	-0.49532954275422725
1103	82-83	-0.49488791008082345
1103	84-85	-0.5948359345476923
1103	86-87	-0.5991555192113864
1103	88-89	-0.64169791498351
1103	90-91	-0.5778017539612179
1103	92-93	-0.5984048450396067
1103	94-95	-0.6160329686205017
1103	96-97	-0.5895260857839588
1103	98-99	-0.6774083061990481
1103	100	-0.6111027816624279
1104	1	-0.19429307805453178
1104	2	-0.07483904853727807
1104	3	-0.010501202716920233
1104	4	0.0438292655820689
1104	5	-0.008515428684141568
1104	6	0.04534309509858758
1104	7	0.045972001393643325
1104	8	0.003262068766112236
1104	9	-0.06353933335978468
1104	10-11	-0.046835508806410076
1104	12-13	-0.02530614625711536
1104	14-15	-0.11526965231642095
1104	16-17	-0.006060489874862185
1104	18-19	-0.05717753658308311
1104	20-21	-0.1104592883515565
1104	22-23	0.013828234075248247
1104	24-25	-0.021508784956125737
1104	26-27	9.641194274649934E-4
1104	28-29	0.026833633035259652
1104	30-31	-0.03237940323221267
1104	32-33	-0.05269732443771602
1104	34-35	-0.013634669409441358
1104	36-37	-0.14363515536497573
1104	38-39	-0.08506502154358486
1104	40-41	-0.07671274312061627
1104	42-43	-0.17110918315643886
1104	44-45	-0.18982040618907092
1104	46-47	-0.2895072949377706
1104	48-49	-0.2826361789233758
1104	50-51	-0.33655545048574
1104	52-53	-0.36167242169982217
1104	54-55	-0.2558292585620663
1104	56-57	-0.3140956268647841
1104	58-59	-0.4180180008940724
1104	60-61	-0.43688958583301485
1104	62-63	-0.38259843689937867
1104	64-65	-0.3431234030407282
1104	66-67	-0.2512103788524698
1104	68-69	-0.28254046936407207
1104	70-71	-0.3484819360481808
1104	72-73	-0.3562081194372624
1104	74-75	-0.2225194616408359
1104	76-77	-0.2323052841901898
1104	78-79	-0.23220377225577238
1104	80-81	-0.2583999131878443
1104	82-83	-0.2939528493563195
1104	84-85	-0.2843450133397205
1104	86-87	-0.2931937366181785
1104	88-89	-0.33961675077226516
1104	90-91	-0.40768540441620615
1104	92-93	-0.4350833145374722
1104	94-95	-0.4805975040328576
1104	96-97	-0.4134994394714653
1104	98-99	-0.41519129987642245
1104	100	-0.3567361356821017
1105	1	-0.1690676422838493
1105	2	-0.09634756868230099
1105	3	-0.10780269877246695
1105	4	-0.0823441065791144
1105	5	-0.06653233594114738
1105	6	-0.11884969399487488
1105	7	-0.08937029354881076
1105	8	-0.09847937060354894
1105	9	-0.16141880965702882
1105	10-11	-0.1323393445311325
1105	12-13	-0.116895277061559
1105	14-15	-0.13264947583450493
1105	16-17	-0.1593026110273641
1105	18-19	-0.11175764562966606
1105	20-21	-0.05259317105765149
1105	22-23	-0.14257145399218984
1105	24-25	-0.17011324300850816
1105	26-27	-0.09519746987273692
1105	28-29	-0.10571074453258689
1105	30-31	-0.11555505711724123
1105	32-33	-0.15375130749768573
1105	34-35	-0.15209416103171947
1105	36-37	-0.1930414067948476
1105	38-39	-0.20344697285385394
1105	40-41	-0.2108713431821272
1105	42-43	-0.22545615064288427
1105	44-45	-0.23649378373602303
1105	46-47	-0.1875933038847677
1105	48-49	-0.29608279303907636
1105	50-51	-0.24863304870624603
1105	52-53	-0.21422205982692333
1105	54-55	-0.21519950545022226
1105	56-57	-0.31937831609877065
1105	58-59	-0.18341469990695458
1105	60-61	-0.2528847701288939
1105	62-63	-0.1967937968909368
1105	64-65	-0.19713192801913237
1105	66-67	-0.28047546196866335
1105	68-69	-0.2509403341030705
1105	70-71	-0.14508878556516436
1105	72-73	-0.14094946867588476
1105	74-75	-0.16752629419955412
1105	76-77	-0.1525831266943527
1105	78-79	-0.1911011534197442
1105	80-81	-0.20195902170257796
1105	82-83	-0.1635463910577606
1105	84-85	-0.1480725228147577
1105	86-87	-0.13574166050633707
1105	88-89	-0.14965929326665162
1105	90-91	-0.14574674747178662
1105	92-93	-0.24958333861231097
1105	94-95	-0.20317494008193648
1105	96-97	-0.31365414677437187
1105	98-99	-0.2588678871562742
1105	100	-0.265606935555013
1106	1	-0.06183155743589808
1106	2	-0.03318050562758401
1106	3	0.004271363080313506
1106	4	0.10388082275292021
1106	5	0.03271596032431745
1106	6	0.05146067103442675
1106	7	0.04068439870583518
1106	8	-0.012702980577564915
1106	9	0.08916329456648242
1106	10-11	0.025136251903440154
1106	12-13	0.0639003875232973
1106	14-15	0.08572544116407954
1106	16-17	0.039802417831452885
1106	18-19	-0.02716104444321843
1106	20-21	-0.05096948205573426
1106	22-23	0.06651747008840658
1106	24-25	0.03620911781741398
1106	26-27	-0.017833374895211307
1106	28-29	-0.022209351300276126
1106	30-31	0.023007262783124816
1106	32-33	-0.0357201542038581
1106	34-35	0.00267393323061782
1106	36-37	0.039905058209264155
1106	38-39	0.035648207752785765
1106	40-41	0.02493702895589678
1106	42-43	-0.007880244911000034
1106	44-45	-0.048516359217096294
1106	46-47	-0.017597480016625866
1106	48-49	0.05183670220404224
1106	50-51	0.05185508352487034
1106	52-53	-0.01349889269256721
1106	54-55	0.04100208871839328
1106	56-57	-0.01619232777478885
1106	58-59	-0.09334129177630501
1106	60-61	-0.13036512123444766
1106	62-63	-0.017664873283280258
1106	64-65	0.03464758702067172
1106	66-67	0.03720409342447084
1106	68-69	0.05454429801148564
1106	70-71	-0.004553092183321894
1106	72-73	0.014939703084444034
1106	74-75	0.024557832970387494
1106	76-77	0.011693497229316563
1106	78-79	0.034550528933209534
1106	80-81	0.10055733691983448
1106	82-83	0.04446973104955276
1106	84-85	0.007417019210603115
1106	86-87	-0.024410121125647777
1106	88-89	-0.03040837913422223
1106	90-91	-0.032560893177539185
1106	92-93	0.009528731545984215
1106	94-95	-0.024146335699715138
1106	96-97	-0.01916991485365216
1106	98-99	-0.023947258933183946
1106	100	-0.055109853116078966
1107	1	-0.32198859705472316
1107	2	-0.3768947258910629
1107	3	-0.3600846264384181
1107	4	-0.28069660632795745
1107	5	-0.2603614504999072
1107	6	-0.31631724367130687
1107	7	-0.3246331226060519
1107	8	-0.3191070063441046
1107	9	-0.32661630172055567
1107	10-11	-0.24389551329947068
1107	12-13	-0.2875324821186851
1107	14-15	-0.34043437114743114
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2216	56-57	0.5273031968324418
2216	58-59	0.5765425255451078
2216	60-61	0.5604445819019617
2216	62-63	0.5528189905478058
2216	64-65	0.4758718995709046
2216	66-67	0.45335742724952155
2216	68-69	0.45409947522383476
2216	70-71	0.3871775053827804
2216	72-73	0.444323896718295
2216	74-75	0.4643628257152628
2216	76-77	0.5088018100973386
2216	78-79	0.5370289547961562
2216	80-81	0.4452777920282216
2216	82-83	0.48208927131127766
2216	84-85	0.5145575580964881
2216	86-87	0.45624754939574075
2216	88-89	0.4643344797691853
2216	90-91	0.5446476398078381
2216	92-93	0.5044643795452544
2216	94-95	0.5203169197314566
2216	96-97	0.4879854404508315
2216	98-99	0.42572833176106784
2216	100	0.40902114320578065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104208.0
3	19244.0
4	13124.0
5	10813.0
6	7948.0
7	7285.0
8	8044.0
9	8562.0
10	8925.0
11	9693.0
12	11113.0
13	12845.0
14	14269.0
15	14904.0
16	15169.0
17	14890.0
18	14424.0
19	13259.0
20	12560.0
21	12997.0
22	15285.0
23	21483.0
24	33350.0
25	44749.0
26	48485.0
27	38891.0
28	39140.0
29	48823.0
30	58367.0
31	66274.0
32	84511.0
33	112587.0
34	159707.0
35	246921.0
36	420283.0
37	789111.0
38	1279595.0
39	677161.0
40	2452.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2579100612394	21.477925876819718	13.965170715430117	22.298993346510766
2	14.60899350390386	22.336068842666855	40.97131260136303	22.083625052066257
3	17.20138634831148	28.875350304230373	30.947316592593214	22.975946754864925
4	11.607619816557017	18.892828078204662	40.00213146060221	29.497420644636108
5	11.639325372115646	40.399848428106395	35.522978432028644	12.437847767749313
6	27.21287171884538	40.88368343677885	19.107578179538816	12.795866664836947
7	24.53790682437936	35.47955696426674	23.48706927081489	16.495466940539007
8	23.95408617749612	37.493817037423106	21.39945755914896	17.152639225931814
9	24.892115848325727	18.322468938216353	21.854430088414105	34.93098512504382
10-11	22.10935941172884	29.205341026207687	29.817409695026946	18.867889867036524
12-13	22.879431506487006	27.41058526351388	29.277739057723817	20.432244172275297
14-15	20.73069030296699	29.04431098071642	26.748066949844272	23.476931766472315
16-17	19.89005079175381	32.10419502262944	27.423828967898285	20.581925217718467
18-19	19.955358674349608	30.950846956831597	29.30677576531544	19.78701860350336
20-21	21.707093449251047	29.95183313515108	28.51562504322845	19.82544837236943
22-23	21.261207325845184	29.983621977535552	28.614861948763348	20.14030874785592
24-25	20.888128372828845	30.02507371725992	28.578095145561015	20.508702764350215
26-27	20.602321211862318	30.03957883795983	28.62603310129478	20.732066848883075
28-29	20.54529354102837	30.026590076151553	28.828376706172577	20.5997396766475
30-31	20.801528132613754	30.34796336598654	28.50050327223791	20.35000522916179
32-33	20.408700333823766	30.215837290101323	28.917670637175675	20.45779173889924
34-35	20.72103337522754	29.93108980016385	28.783656234503397	20.56422059010521
36-37	20.901445339378444	30.020644859387957	28.48918654516972	20.588723256063883
38-39	20.71426010135889	30.036214465068017	28.729507761286005	20.520017672287093
40-41	20.63420006648904	29.847679457321963	28.864033458771267	20.654087017417737
42-43	20.564440890163038	29.825698797375487	28.884370042601944	20.725490269859527
44-45	20.72783946724913	29.917506450360037	28.773326417825718	20.58132766456512
46-47	20.737891055886447	30.12429478323587	28.619341706566743	20.518472454310942
48-49	20.70848616186949	29.898319679298034	28.64864894777968	20.744545211052795
50-51	20.616275088047363	29.86867988611117	28.651721580197524	20.86332344564394
52-53	20.671107841573722	29.888085978159495	28.64982800804418	20.790978172222598
54-55	20.758452393745976	30.137396256656736	28.53585104542699	20.568300304170297
56-57	20.726283920645443	29.903764398017586	28.444985880450236	20.924965800886735
58-59	20.690082231648677	29.698597582888237	28.690665276272732	20.920654909190358
60-61	20.849295520413136	30.036476514156053	28.3389169400367	20.775311025394114
62-63	20.7059050062054	29.936750849472997	28.564126427477056	20.79321771684455
64-65	20.740783039890363	29.80684954064599	28.57854125775439	20.873826161709257
66-67	20.614660689968854	29.877542618831686	28.462656613036113	21.045140078163342
68-69	20.989520727146047	29.985224063705623	28.29269766411393	20.732557545034403
70-71	20.888911217546017	29.81278158729751	28.40940704357489	20.88890015158158
72-73	20.781058839568207	29.918959042646424	28.610392576579258	20.68958954120611
74-75	20.78959203511462	29.98536817055875	28.441146891084834	20.7838929032418
76-77	20.871329081963246	29.968529178156373	28.253597918279894	20.90654382160049
78-79	20.908706905719647	29.91615336187205	28.477059229144768	20.69808050326353
80-81	20.84184824526134	29.896623573880216	28.464900965833113	20.796627215025335
82-83	20.735720585414743	29.84045180512545	28.68579394542263	20.73803366403717
84-85	20.776764789702753	29.759485580368384	28.445845104131795	21.01790452579707
86-87	20.97363392504774	29.838316080806766	28.324168654873993	20.863881339271508
88-89	20.814546442963774	29.6997550565787	28.48825241080903	20.997446089648495
90-91	20.736309516760773	29.73530251244202	28.407460132357045	21.120927838440164
92-93	20.8506063468192	29.808482160395155	28.343887757366105	20.99702373541954
94-95	20.72522215013242	29.759945395204753	28.483786268881474	21.031046185781353
96-97	20.76089102380803	29.848241863989795	28.348997013926454	21.041870098275727
98-99	20.745349355049772	29.80425722929488	28.344453622815006	21.10593979284034
100	20.770573064297874	29.98277152447105	28.151877987949586	21.094777423281485
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15079.0
1	11118.0
2	6517.0
3	5091.5
4	3682.5
5	2763.0
6	2369.5
7	2399.0
8	2736.0
9	2859.0
10	2264.5
11	1724.0
12	1651.5
13	1693.5
14	1824.5
15	1964.5
16	2268.0
17	2794.0
18	3664.0
19	4953.0
20	6911.5
21	9899.5
22	14728.5
23	22071.0
24	30067.0
25	38389.5
26	49785.0
27	63814.5
28	81387.5
29	98556.5
30	109369.5
31	120082.5
32	132345.5
33	143146.5
34	152133.5
35	157845.0
36	160220.0
37	161304.5
38	161417.5
39	160057.0
40	158474.5
41	155102.5
42	148291.0
43	141810.5
44	137658.0
45	133914.0
46	131352.5
47	127664.0
48	122841.5
49	120068.5
50	118017.0
51	113143.0
52	106141.0
53	100109.5
54	95853.5
55	92896.0
56	85782.0
57	76539.5
58	69142.0
59	62198.0
60	54636.0
61	45916.5
62	38552.5
63	31354.0
64	24301.0
65	19249.0
66	15174.0
67	11667.0
68	9001.5
69	6833.5
70	5040.5
71	3765.5
72	2850.0
73	2226.5
74	1830.0
75	1463.0
76	1062.5
77	793.0
78	616.5
79	470.0
80	350.5
81	265.0
82	214.0
83	173.0
84	149.5
85	129.5
86	121.0
87	107.5
88	89.5
89	75.0
90	68.0
91	66.5
92	61.0
93	49.5
94	39.5
95	37.5
96	36.5
97	36.5
98	34.0
99	33.5
100	62.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18091537429024443
2	0.17629296436033476
3	0.18993902621083364
4	0.17945566589132558
5	0.1933228956810546
6	0.07636928941616308
7	0.07809439934215807
8	0.06604074665411612
9	0.060047095500979655
10-11	0.07365998216059402
12-13	0.07218921536471368
14-15	0.0708400909354099
16-17	0.06637249856296132
18-19	0.07112760925640906
20-21	0.07306282872467268
22-23	0.07079585734756387
24-25	0.07019870391164253
26-27	0.07475476345978316
28-29	0.07130454360779316
30-31	0.07781793941812042
32-33	0.07405808445120826
34-35	0.07352728139705594
36-37	0.0854039997337138
38-39	0.08057148026153552
40-41	0.07548461765924258
42-43	0.07922235583223174
44-45	0.08996005928185444
46-47	0.07967575010765349
48-49	0.08539294133675229
50-51	0.0820975390422234
52-53	0.08078158980380414
54-55	0.0829711524021824
56-57	0.07573896078935723
58-59	0.08374524018948784
60-61	0.07989691804688362
62-63	0.07096173330198646
64-65	0.07446724513878399
66-67	0.07405808445120826
68-69	0.07285271918240406
70-71	0.06838512680995548
72-73	0.06623979779942324
74-75	0.0711939596381781
76-77	0.07644669819489364
78-79	0.07213392337990614
80-81	0.08053830507065099
82-83	0.08100275774303427
84-85	0.08268363408118323
86-87	0.07878001995377147
88-89	0.08123498407922589
90-91	0.07952093255019241
92-93	0.0708511493323714
94-95	0.07003282795721993
96-97	0.06951308330002913
98-99	0.07924447262615475
100	0.08691900011744018
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4521451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.878123998140225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1838923256371	47.90300228312373
2	14.61455026000731	18.37871006851584
3	4.502429103217704	8.49312886334874
4	1.87046750731961	4.704459514389387
5	0.9212890723921825	2.896446426600362
6	0.4976570587085961	1.877504534761731
7	0.30802685639828586	1.3557705609958124
8	0.21708178715665016	1.0919756424458984
9	0.15121807325718686	0.8557477884922721
>10	0.6750645584864026	7.299191098411605
>50	0.037091923684535844	1.6135709192497005
>100	0.019648655477991345	2.370654836295896
>500	0.0011255600905446144	0.46161747843736056
>1k	4.2208446073591685E-4	0.3631540118019684
>5k	0.0	0.0
>10k+	3.5173705061241E-5	0.3350659731296806
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15150	0.3350694279336434	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.2116793923012767E-5	0.0
4	0.0	0.0	0.0	2.2116793923012767E-5	0.0
5	0.0	0.0	0.0	2.2116793923012767E-5	0.0
6	0.0	0.0	0.0	2.2116793923012767E-5	0.0
7	0.0	0.0	0.0	2.2116793923012767E-5	0.0
8	0.0	0.0	0.0	4.4233587846025534E-5	0.0
9	0.0	0.0	0.0	4.4233587846025534E-5	0.0
10-11	0.0	0.0	0.0	4.4233587846025534E-5	2.2116793923012767E-5
12-13	0.0	0.0	0.0	6.63503817690383E-5	2.2116793923012767E-5
14-15	0.0	0.0	0.0	6.63503817690383E-5	2.2116793923012767E-5
16-17	0.0	0.0	0.0	9.952557265355746E-5	2.2116793923012767E-5
18-19	0.0	0.0	0.0	1.2164236657657022E-4	2.2116793923012767E-5
20-21	0.0	0.0	0.0	1.5481755746108938E-4	2.2116793923012767E-5
22-23	0.0	0.0	0.0	2.543431301146468E-4	2.2116793923012767E-5
24-25	0.0	0.0	0.0	5.308030541523064E-4	2.2116793923012767E-5
26-27	0.0	0.0	0.0	0.001006314123497081	2.2116793923012767E-5
28-29	0.0	0.0	0.0	0.002333321758877847	2.2116793923012767E-5
30-31	0.0	0.0	0.0	0.006469162222481235	2.2116793923012767E-5
32-33	0.0	0.0	0.0	0.01394463856845955	2.2116793923012767E-5
34-35	0.0	0.0	1.1058396961506384E-5	0.024958801942119906	2.2116793923012767E-5
36-37	0.0	0.0	2.2116793923012767E-5	0.04240895234737698	2.2116793923012767E-5
38-39	0.0	0.0	2.2116793923012767E-5	0.06900439703979983	2.2116793923012767E-5
40-41	0.0	0.0	2.2116793923012767E-5	0.09626334554991306	2.2116793923012767E-5
42-43	0.0	0.0	2.2116793923012767E-5	0.12280349825752838	2.2116793923012767E-5
44-45	0.0	0.0	2.2116793923012767E-5	0.15757109830450447	2.2116793923012767E-5
46-47	0.0	0.0	2.2116793923012767E-5	0.20183786134141452	2.2116793923012767E-5
48-49	0.0	0.0	2.2116793923012767E-5	0.24846006293112544	2.2116793923012767E-5
50-51	0.0	0.0	2.2116793923012767E-5	0.29882000269382547	2.2116793923012767E-5
52-53	0.0	0.0	2.2116793923012767E-5	0.3487265481811038	2.2116793923012767E-5
54-55	0.0	0.0	2.2116793923012767E-5	0.40320021161348424	2.2116793923012767E-5
56-57	0.0	0.0	2.2116793923012767E-5	0.47130887849940206	2.2116793923012767E-5
58-59	0.0	0.0	2.2116793923012767E-5	0.5516149572338614	2.2116793923012767E-5
60-61	0.0	0.0	2.2116793923012767E-5	0.6375497600217275	2.2116793923012767E-5
62-63	0.0	0.0	2.2116793923012767E-5	0.7235730299852857	2.2116793923012767E-5
64-65	0.0	0.0	2.2116793923012767E-5	0.8162202797287862	2.2116793923012767E-5
66-67	0.0	0.0	2.2116793923012767E-5	0.9226352336893622	2.2116793923012767E-5
68-69	0.0	0.0	2.2116793923012767E-5	1.0512112151607969	2.2116793923012767E-5
70-71	0.0	0.0	2.2116793923012767E-5	1.193355849704	2.2116793923012767E-5
72-73	0.0	0.0	2.2116793923012767E-5	1.3425667999056055	2.2116793923012767E-5
74-75	0.0	0.0	2.2116793923012767E-5	1.496510744006736	4.4233587846025534E-5
76-77	0.0	0.0	2.2116793923012767E-5	1.6641560419431727	4.4233587846025534E-5
78-79	0.0	0.0	2.2116793923012767E-5	1.8589607628170692	4.4233587846025534E-5
80-81	0.0	0.0	2.2116793923012767E-5	2.079133446320661	4.4233587846025534E-5
82-83	0.0	0.0	2.2116793923012767E-5	2.3099443077012225	4.4233587846025534E-5
84-85	0.0	0.0	2.2116793923012767E-5	2.5432875419859684	4.4233587846025534E-5
86-87	0.0	0.0	2.2116793923012767E-5	2.7940366930881257	4.4233587846025534E-5
88	0.0	0.0	2.2116793923012767E-5	3.002244191079368	4.4233587846025534E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6080	0.0	47.284462	1
GTATCAA	10515	0.0	37.722385	1
TCAACGC	11815	0.0	32.333313	4
ATCAACG	11975	0.0	31.981295	3
TATCAAC	12875	0.0	30.44002	2
CAACGCA	12575	0.0	30.341087	5
AACGCAG	13425	0.0	28.45987	6
GTACATG	22845	0.0	28.031595	1
TACATGG	22820	0.0	27.235435	2
CATGGGT	10430	0.0	26.568003	4
ACATGGG	23175	0.0	25.823467	3
CGCAGAG	15025	0.0	24.897747	8
ACGCAGA	15285	0.0	24.750952	7
ATGGGTG	5745	0.0	21.864498	5
GCAGAGT	16905	0.0	21.239273	9
ATGGGTA	4855	0.0	21.124456	5
GTGGTAT	3025	0.0	20.687458	1
TACCTGG	3545	0.0	20.438623	2
GTACCTG	3820	0.0	20.323637	1
CATGGGG	10035	0.0	18.706108	4
>>END_MODULE