FastQCFastQC Report
Wed 25 May 2016
SRR2431389_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431389_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4521451
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279990.6192481130504345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226240.5003703457142408No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150720.3333443180076484No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47670.10543075663100186No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47120.10421433296523616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118000.063.721521
GTATCAA228850.044.671271
TATCAAC293750.034.6405372
ATCAACG292950.034.6044273
TCAACGC296200.034.2081074
CAACGCA306650.033.0270355
GTGGTAT39200.032.2690281
AACGCAG318500.031.9320856
TGGTATC39850.031.5056292
CGCAGAG356350.028.2898068
ACGCAGA358250.028.2447227
CATGGGT101650.028.0305794
GTACATG215550.027.0516241
TACATGG216600.026.4202772
GCAGAGT387500.025.7367159
ACATGGG217750.025.4584543
ATGGGTG57150.024.7637885
TACCTGG35800.023.773922
GAGTACT265950.023.04395712-13
CAGAGTA382250.022.34019310-11
Produced by FastQC (version 0.11.5)