##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431389_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4521451
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.66143412811507	34.0	31.0	34.0	31.0	34.0
2	32.78918515317317	34.0	31.0	34.0	31.0	34.0
3	32.873597878203256	34.0	31.0	34.0	31.0	34.0
4	36.335635617858074	37.0	37.0	37.0	35.0	37.0
5	36.241608501341716	37.0	37.0	37.0	35.0	37.0
6	36.28055971412717	37.0	37.0	37.0	35.0	37.0
7	36.237282898786255	37.0	37.0	37.0	35.0	37.0
8	36.25293406917381	37.0	37.0	37.0	35.0	37.0
9	38.05764808686415	39.0	39.0	39.0	35.0	39.0
10-11	38.03245009179575	39.0	39.0	39.0	35.0	39.0
12-13	38.01616217891115	39.0	39.0	39.0	35.0	39.0
14-15	39.497427706282785	41.0	39.0	41.0	36.0	41.0
16-17	39.527282502895645	41.0	39.0	41.0	36.5	41.0
18-19	39.59106180737113	41.0	40.0	41.0	37.0	41.0
20-21	39.58611041013161	41.0	40.0	41.0	37.0	41.0
22-23	39.530326105491355	41.0	40.0	41.0	36.0	41.0
24-25	39.48293877341588	41.0	39.5	41.0	36.0	41.0
26-27	39.373578747176516	41.0	39.0	41.0	36.0	41.0
28-29	39.29924586156081	41.0	39.0	41.0	36.0	41.0
30-31	39.135995281160845	41.0	39.0	41.0	35.0	41.0
32-33	38.93357265178811	41.0	38.5	41.0	35.0	41.0
34-35	38.807364715442006	41.0	38.0	41.0	35.0	41.0
36-37	38.67582276132153	41.0	38.0	41.0	35.0	41.0
38-39	38.53650166727451	41.0	38.0	41.0	35.0	41.0
40-41	38.40580579110555	41.0	38.0	41.0	35.0	41.0
42-43	38.24796895952207	41.0	38.0	41.0	34.0	41.0
44-45	38.02149774486111	40.0	38.0	41.0	34.0	41.0
46-47	37.68028626208711	40.0	38.0	41.0	33.0	41.0
48-49	37.36843073163902	40.0	38.0	41.0	33.0	41.0
50-51	37.216362623414476	40.0	37.5	41.0	32.0	41.0
52-53	37.1606416833888	40.0	37.0	41.0	32.0	41.0
54-55	37.02542380753435	40.0	37.0	41.0	32.0	41.0
56-57	36.881078441411844	40.0	36.5	41.0	31.5	41.0
58-59	36.71383788080419	40.0	36.0	41.0	31.0	41.0
60-61	36.56607436418088	40.0	36.0	41.0	31.0	41.0
62-63	36.41400902055557	39.5	35.0	41.0	31.0	41.0
64-65	36.18703044664202	39.0	35.0	41.0	31.0	41.0
66-67	35.92943006570236	39.0	35.0	41.0	31.0	41.0
68-69	35.63868037052707	38.5	35.0	41.0	30.0	41.0
70-71	35.33741004823452	37.0	35.0	40.5	30.0	41.0
72-73	35.01765738476432	37.0	35.0	40.0	30.0	41.0
74-75	34.67773265706076	36.5	35.0	39.0	29.0	41.0
76-77	34.335492964537266	36.0	35.0	39.0	29.0	41.0
78-79	34.006016873786756	36.0	35.0	38.5	29.0	40.0
80-81	33.72823027386562	35.0	35.0	37.0	29.0	39.0
82-83	33.51029459348338	35.0	35.0	37.0	29.0	39.0
84-85	33.236291734666594	35.0	34.5	36.5	29.0	39.0
86-87	32.98048226111485	35.0	34.0	36.0	29.0	37.0
88-89	32.738740063753866	35.0	34.0	36.0	29.0	37.0
90-91	32.551797752535634	35.0	34.0	36.0	29.0	37.0
92-93	32.38484858068793	35.0	34.0	35.0	29.0	36.0
94-95	32.2425082125185	35.0	34.0	35.0	29.0	36.0
96-97	32.09932939669146	35.0	34.0	35.0	29.0	36.0
98-99	31.982013296174173	35.0	34.0	35.0	27.5	36.0
100	31.87973064399017	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.018377865270878146
1101	2	0.033040574867037265
1101	3	0.017447123653766994
1101	4	0.03469913449161055
1101	5	0.05118036929846426
1101	6	0.02961442157906191
1101	7	0.03668728611068417
1101	8	0.05570337828931571
1101	9	0.09146418112616317
1101	10-11	0.0609257431880863
1101	12-13	0.04542140114169513
1101	14-15	0.06372250872621521
1101	16-17	0.039710608888476884
1101	18-19	0.055730588530572334
1101	20-21	0.069962573746011
1101	22-23	0.05656638026908212
1101	24-25	0.0659042657849298
1101	26-27	0.11386270509619578
1101	28-29	0.0716458304627281
1101	30-31	0.021916948113720025
1101	32-33	0.06976527000862376
1101	34-35	0.11241357958713394
1101	36-37	0.06036057948001172
1101	38-39	0.11214349695628556
1101	40-41	0.0473021160418341
1101	42-43	0.03637494138668984
1101	44-45	0.05048530463159295
1101	46-47	0.07975768957089713
1101	48-49	0.12101550046019582
1101	50-51	0.09998499368521863
1101	52-53	0.11094698076758647
1101	54-55	0.14335477242929784
1101	56-57	0.15794823000000946
1101	58-59	0.15031877765945012
1101	60-61	0.1512527429712236
1101	62-63	0.1798608887202704
1101	64-65	0.17566964349641978
1101	66-67	0.1480162101461957
1101	68-69	0.16113765253154355
1101	70-71	0.1970841425052754
1101	72-73	0.17965748699898398
1101	74-75	0.13155252006239238
1101	76-77	0.16112266832171684
1101	78-79	0.18452283229532895
1101	80-81	0.19141675303058747
1101	82-83	0.18730620698408273
1101	84-85	0.1917836171216365
1101	86-87	0.20198249445023464
1101	88-89	0.21944226354876406
1101	90-91	0.18282203873813785
1101	92-93	0.20946368830960438
1101	94-95	0.18943923313705824
1101	96-97	0.2123271719304114
1101	98-99	0.17771883995290594
1101	100	0.2178739446646567
1102	1	-0.0591164037409726
1102	2	-0.03723877544364029
1102	3	-0.004519088763103696
1102	4	0.0265028152959772
1102	5	-0.02236181008805005
1102	6	-0.03054605339298888
1102	7	-0.04712229715912741
1102	8	0.0058824333460592015
1102	9	7.088215370600892E-4
1102	10-11	0.010808643920150018
1102	12-13	-0.020662469164378194
1102	14-15	-0.07995710874104134
1102	16-17	-0.07203672562162922
1102	18-19	-0.06098169032088663
1102	20-21	-0.008011514959974875
1102	22-23	-0.013284548814738173
1102	24-25	0.01630719460057861
1102	26-27	0.03811673461598275
1102	28-29	0.00785903297310142
1102	30-31	0.02977361398191647
1102	32-33	0.007248686162306228
1102	34-35	-0.0063498993723456465
1102	36-37	0.014241254754630006
1102	38-39	0.027711837560083552
1102	40-41	0.06387631703749008
1102	42-43	0.04389462658599541
1102	44-45	-0.05844035576274109
1102	46-47	-0.1094530420488411
1102	48-49	-0.10575381672590112
1102	50-51	-0.16276032697073362
1102	52-53	-0.19320095679882598
1102	54-55	-0.1565161155934618
1102	56-57	-0.16656789585953646
1102	58-59	-0.11378807841294503
1102	60-61	-0.1342387668953151
1102	62-63	-0.15262281239819941
1102	64-65	-0.1289901022437192
1102	66-67	-0.13348329812026805
1102	68-69	-0.12038327263879012
1102	70-71	-0.06744641903644322
1102	72-73	-0.12443054738038484
1102	74-75	-0.1615318702123716
1102	76-77	-0.16923620985443932
1102	78-79	-0.09651076625362975
1102	80-81	-0.14753593399974108
1102	82-83	-0.10846102170347649
1102	84-85	-0.08495773930460615
1102	86-87	-0.05533605834483524
1102	88-89	-0.10119867370720925
1102	90-91	-0.14738696862483636
1102	92-93	-0.1277198888141342
1102	94-95	-0.11067548449513964
1102	96-97	-0.12089124896360914
1102	98-99	-0.101533687057632
1102	100	-0.07940420323475195
1103	1	-0.05315935553868911
1103	2	-0.07603474401764032
1103	3	-0.04551938078814288
1103	4	0.021341445791655644
1103	5	-0.03238937218993243
1103	6	-0.023194543828864767
1103	7	-0.04219707873131995
1103	8	0.022750879049340256
1103	9	0.03150762583420175
1103	10-11	0.02981769526379452
1103	12-13	0.02394991952644432
1103	14-15	-0.02101649822961349
1103	16-17	0.007749386928111335
1103	18-19	-0.07078991129143475
1103	20-21	-0.04689303755895935
1103	22-23	-0.040781595732390485
1103	24-25	-0.0166648443001165
1103	26-27	-0.05106361818751992
1103	28-29	-0.025395408231126737
1103	30-31	-0.03863099566799377
1103	32-33	-0.07938142603692455
1103	34-35	-0.03477784476008594
1103	36-37	-0.12758585257137867
1103	38-39	-0.14181784452898683
1103	40-41	-0.1696735398511393
1103	42-43	-0.12126140146273201
1103	44-45	-0.14128579476377467
1103	46-47	-0.09828597340002432
1103	48-49	-0.06278991297907766
1103	50-51	-0.14733803798628742
1103	52-53	-0.0389946858774195
1103	54-55	0.055469661855759966
1103	56-57	0.01979197975641256
1103	58-59	-0.03583088312364424
1103	60-61	-0.0692494175715197
1103	62-63	-0.05306354814848646
1103	64-65	-0.09015467566628388
1103	66-67	-0.009289620331095705
1103	68-69	-0.07479036748627976
1103	70-71	-0.12785096326128098
1103	72-73	-0.1183443682355616
1103	74-75	-0.09217525414984351
1103	76-77	-0.07965455542632327
1103	78-79	-0.04142108973285019
1103	80-81	-0.04362977074318053
1103	82-83	-0.08059459773039634
1103	84-85	-0.06314769502807138
1103	86-87	-0.03270103735508201
1103	88-89	-0.04565165445194452
1103	90-91	-0.05067273984195708
1103	92-93	-0.08723872323546544
1103	94-95	-0.07233630946708303
1103	96-97	-0.07353575897639075
1103	98-99	-0.0632398797793563
1103	100	-0.046202842297770275
1104	1	-0.11982727757684586
1104	2	-0.07134076187089278
1104	3	-0.11598480937782085
1104	4	-0.055155309852779055
1104	5	-0.05473749543684647
1104	6	-0.10639851541879608
1104	7	-0.0740661732708503
1104	8	-0.09484499254066492
1104	9	-0.08896211858230174
1104	10-11	-0.14281330762442224
1104	12-13	-0.1142369957755136
1104	14-15	-0.11427412408509241
1104	16-17	-0.10451556132991868
1104	18-19	-0.06541227410486528
1104	20-21	-0.05192973612220442
1104	22-23	-0.048831969501712535
1104	24-25	-0.04016137514728513
1104	26-27	-0.09310235630057662
1104	28-29	-0.08899864877379571
1104	30-31	-0.026250958694575388
1104	32-33	0.02625933623400556
1104	34-35	-0.028564609698172205
1104	36-37	-0.07513159741174746
1104	38-39	-0.10924581992901494
1104	40-41	-0.166187685119759
1104	42-43	-0.12717570996083793
1104	44-45	-0.13315904606442786
1104	46-47	-0.15843546475235826
1104	48-49	-0.12435110076012279
1104	50-51	-0.21823549370695616
1104	52-53	-0.15339476372605532
1104	54-55	-0.09763662233898174
1104	56-57	-0.10911554529116785
1104	58-59	-0.1086089399161807
1104	60-61	-0.13794739226744213
1104	62-63	-0.2250229545807798
1104	64-65	-0.1426672588161253
1104	66-67	-0.17125108058232996
1104	68-69	-0.2294132379064493
1104	70-71	-0.22841628979159623
1104	72-73	-0.21688781761746156
1104	74-75	-0.18580090658239357
1104	76-77	-0.2255815026303125
1104	78-79	-0.21852510209506448
1104	80-81	-0.24744388562557873
1104	82-83	-0.28943044028622467
1104	84-85	-0.28576964520635784
1104	86-87	-0.25846231294974586
1104	88-89	-0.21325386527809798
1104	90-91	-0.21497301565721472
1104	92-93	-0.208044564128123
1104	94-95	-0.24320086431819732
1104	96-97	-0.2015756624796481
1104	98-99	-0.27059480893312937
1104	100	-0.19841463921858704
1105	1	-0.16347302558759225
1105	2	-0.14008724443578302
1105	3	-0.12580939173001582
1105	4	-0.07358287216356985
1105	5	-0.10714406780318342
1105	6	-0.04205296388266788
1105	7	-0.0460899455853081
1105	8	-0.06595021541796342
1105	9	-0.018268911470471494
1105	10-11	-0.09733334925962112
1105	12-13	-0.043686580532892094
1105	14-15	-0.12889744769611866
1105	16-17	-0.17452268158985618
1105	18-19	-0.17013349254393262
1105	20-21	-0.11163195109016044
1105	22-23	-0.14363047645667137
1105	24-25	-0.22633314492775725
1105	26-27	-0.10766207492601865
1105	28-29	-0.09913844808999528
1105	30-31	-0.1601111895754883
1105	32-33	-0.17539602983564606
1105	34-35	-0.19464634142566695
1105	36-37	-0.17926866729496993
1105	38-39	-0.08973613277578352
1105	40-41	-0.1667368952395165
1105	42-43	-0.2552234095341106
1105	44-45	-0.20661314915794549
1105	46-47	-0.25025983158180765
1105	48-49	-0.17607690299310974
1105	50-51	-0.19950674274361546
1105	52-53	-0.21965345932279234
1105	54-55	-0.1694021379276478
1105	56-57	-0.1182072605732074
1105	58-59	-0.17037546867345554
1105	60-61	-0.20060509281383077
1105	62-63	-0.24391701866477433
1105	64-65	-0.2640064334202066
1105	66-67	-0.2768956588229301
1105	68-69	-0.1713727851816529
1105	70-71	-0.17800542022777677
1105	72-73	-0.18045590148959434
1105	74-75	-0.2415730308291799
1105	76-77	-0.25173073550838154
1105	78-79	-0.21762193891912318
1105	80-81	-0.16743254237205463
1105	82-83	-0.173668468900388
1105	84-85	-0.18025403766838366
1105	86-87	-0.16946385106952278
1105	88-89	-0.15408280430136756
1105	90-91	-0.16365368921502466
1105	92-93	-0.21578163246466175
1105	94-95	-0.22416589568499745
1105	96-97	-0.1993880054235575
1105	98-99	-0.19848706698997987
1105	100	-0.1788740414463419
1106	1	-0.1272214121538937
1106	2	-0.08155454149648733
1106	3	-0.08648287896077278
1106	4	-0.040030085071656174
1106	5	-0.057944572801950756
1106	6	-0.08602726230470381
1106	7	-0.05926373720345168
1106	8	-0.020381640074617735
1106	9	-0.13118738321380619
1106	10-11	-0.08864475890855061
1106	12-13	-0.10211099918004862
1106	14-15	-0.11519387760429822
1106	16-17	-0.11400340527099218
1106	18-19	-0.12331329609515507
1106	20-21	-0.12088918628147383
1106	22-23	-0.11110819525433868
1106	24-25	-0.05333375758354464
1106	26-27	-0.09194046605655615
1106	28-29	-0.07762855980941907
1106	30-31	-0.1609164172044686
1106	32-33	-0.12107047287440764
1106	34-35	-0.1484933941840083
1106	36-37	-0.11911517481854617
1106	38-39	-0.09204685954998126
1106	40-41	-0.13383634106040887
1106	42-43	-0.12965700980473116
1106	44-45	-0.097645012042193
1106	46-47	-0.13782897761203117
1106	48-49	-0.013698187492558134
1106	50-51	-0.021701633530142317
1106	52-53	0.037910307918203046
1106	54-55	-0.0010733934956306257
1106	56-57	0.02152721027695037
1106	58-59	0.005191276284023161
1106	60-61	0.002908465083535816
1106	62-63	-0.05376784988830963
1106	64-65	-0.03694917627869643
1106	66-67	-0.008830684456910376
1106	68-69	-0.05144864240107694
1106	70-71	-0.09863784020638633
1106	72-73	-1.9305022676974204E-4
1106	74-75	-0.023445843199915828
1106	76-77	-0.055969703394588066
1106	78-79	-0.04614728646905775
1106	80-81	-0.017686873946814785
1106	82-83	-0.04651719012585431
1106	84-85	-0.04011495045509861
1106	86-87	-0.07701221617301712
1106	88-89	-0.10513450223245968
1106	90-91	-0.07179197694319583
1106	92-93	-0.03830119561617806
1106	94-95	-0.058976786498959655
1106	96-97	-0.07973657028099268
1106	98-99	-0.06037516822728861
1106	100	-0.022438529288940856
1107	1	-0.2931767547328761
1107	2	-0.2174297190886847
1107	3	-0.18996256693800717
1107	4	-0.22480940716975084
1107	5	-0.28260314763161176
1107	6	-0.2801221387651154
1107	7	-0.20331080835345716
1107	8	-0.17287545004004556
1107	9	-0.17142317126043594
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2216	32-33	-0.011226358285227889
2216	34-35	0.010657451659589867
2216	36-37	0.02718057034692123
2216	38-39	0.07471894040391192
2216	40-41	-0.00217263922907307
2216	42-43	0.06171116779879071
2216	44-45	0.053595534981738524
2216	46-47	0.10140605113471679
2216	48-49	0.08672622429518384
2216	50-51	0.09385009573386327
2216	52-53	0.044660710389912595
2216	54-55	0.03755709375445804
2216	56-57	0.011988782907259576
2216	58-59	0.030996910482329554
2216	60-61	-0.022080044068047755
2216	62-63	-0.019084027957731564
2216	64-65	0.004327416184622734
2216	66-67	0.02683605358971164
2216	68-69	0.060205841808645744
2216	70-71	0.047703153440785684
2216	72-73	0.006554972132661874
2216	74-75	0.059227761356488884
2216	76-77	0.02675701894468574
2216	78-79	0.03347013094328588
2216	80-81	0.0693151097318605
2216	82-83	0.09434294065174242
2216	84-85	0.10550678379589584
2216	86-87	0.10811189930568332
2216	88-89	0.16140435796636865
2216	90-91	0.1111582880019455
2216	92-93	0.09174347654460036
2216	94-95	0.04205263051212427
2216	96-97	0.06619747686001887
2216	98-99	0.05444876485400485
2216	100	0.07192291091050862
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33.0
3	325.0
4	83.0
5	161.0
6	300.0
7	725.0
8	1426.0
9	2440.0
10	3580.0
11	5132.0
12	7952.0
13	12097.0
14	13478.0
15	17886.0
16	22983.0
17	34920.0
18	41189.0
19	20795.0
20	12772.0
21	11784.0
22	11576.0
23	11484.0
24	12593.0
25	14832.0
26	18259.0
27	23088.0
28	29069.0
29	36799.0
30	46657.0
31	59058.0
32	74728.0
33	97060.0
34	134546.0
35	215509.0
36	353355.0
37	749256.0
38	1437320.0
39	976439.0
40	9762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.62554189153322	21.066929133858267	14.449991152791295	22.857537821817218
2	14.15575065827816	22.047988781900642	40.93599910644477	22.86026145337643
3	16.37098404432961	28.295095193924364	32.215958678303764	23.117962083442258
4	10.848486470383069	18.465952633347126	40.2769376467864	30.40862324948341
5	11.105124562136801	39.32277601119266	35.94632551155675	13.625773915113784
6	27.175347029084246	40.40946147597309	18.71797350009986	13.697217994842806
7	24.005170021747443	35.271088860633455	23.777079526019413	16.94666159159969
8	24.556209942339308	35.682483344395415	21.423189148793163	18.33811756447211
9	25.624500989726744	15.878799942496325	22.42995056895465	36.066748498822285
10-11	22.485381352136738	27.61715210449035	30.096312002496546	19.80115454087637
12-13	24.074450878711282	25.941384745737594	30.142226466680714	19.841937908870406
14-15	21.326306533013405	27.145113371791492	28.681036242569036	22.84754385262607
16-17	19.145048790753233	30.49689137403015	29.290630375072073	21.06742946014454
18-19	19.083521980391026	28.247915132544303	33.12894833195675	19.539614555107924
20-21	20.63633997139414	27.333835974336555	32.86473744822182	19.16508660604748
22-23	20.136109006636037	27.41773406957216	33.04169381964788	19.404463104143925
24-25	19.880805909786883	27.483092256486803	32.941010724659286	19.69509110906703
26-27	19.647066252182515	27.489539568502845	32.977820025839094	19.885574153475545
28-29	19.56375888280836	27.522067339320433	33.120079213622994	19.79409456424822
30-31	19.8618583218418	27.795848451103573	32.77987457549522	19.5624186515594
32-33	19.551472846288597	27.67397078580886	33.09330066792388	19.681255699978667
34-35	19.82336939543353	27.52918971025322	32.83407943113365	19.813361463179604
36-37	20.07868799958243	27.6411227404957	32.43238102526186	19.847808234660008
38-39	19.91668307931784	27.75612831438331	32.50589611361322	19.821292492685636
40-41	19.825458415497348	27.634103175888058	32.53287003001696	20.007568378597636
42-43	19.86545398167437	27.721226064249183	32.27372685658753	20.139593097488913
44-45	20.158944779704804	27.879066081647135	31.89376757803839	20.06822156060967
46-47	20.281874582752167	28.19168258986317	31.34632778226688	20.18011504511778
48-49	20.37525409476576	28.20356244676755	30.902545935003495	20.518637523463195
50-51	20.363472578380147	28.320514596948783	30.642661195550136	20.673351629120933
52-53	20.36951661540881	28.450826460401107	30.623008853080574	20.55664807110951
54-55	20.452406895051585	28.735474822989655	30.444065482576093	20.36805279938267
56-57	20.380810956725103	28.385265819884363	30.428770073369666	20.805153150020868
58-59	20.26757932868738	28.14749463415907	30.807728668757235	20.777197368396315
60-61	20.432571674133747	28.375950286205537	30.500358185438543	20.691119854222176
62-63	20.260198053612573	28.265425193336046	30.749997456541134	20.724379296510243
64-65	20.311533238915246	28.10668735061943	30.829418322438983	20.75236108802634
66-67	20.217656731059623	28.240989606941962	30.628430398646977	20.91292326335144
68-69	20.654741393991376	28.338255075712702	30.32770134430802	20.679302185987897
70-71	20.527283809833865	28.16142924504516	30.35614127900959	20.955145666111385
72-73	20.4221065806233	28.291588898318036	30.47006474263404	20.816239778424624
74-75	20.46153163931669	28.35792754206723	30.271086086052325	20.90945473256376
76-77	20.54274362912596	28.367437505480858	30.115717054600033	20.974101810793144
78-79	20.52112033573579	28.428085249908076	30.2572923837407	20.793502030615432
80-81	20.474178780678713	28.326938214281057	30.273684112746068	20.925198892294166
82-83	20.30611212389131	28.324168548004952	30.476861889035046	20.892857439068692
84-85	20.319368212756437	28.334327311114453	30.188146557849233	21.158157918279876
86-87	20.469230030005324	28.359182277827856	30.08029987407562	21.091287818091196
88-89	20.270104541792662	28.226991765267268	30.240395806827426	21.262507886112644
90-91	20.197894479353128	28.21304554043499	30.16089376751601	21.42816621269587
92-93	20.216008220459052	28.287166237375043	30.130543579181857	21.36628196298405
94-95	20.02751351070406	28.18152060803974	30.311429054842343	21.479536826413856
96-97	20.03358664867682	28.30737865673089	30.103742403276655	21.55529229131563
98-99	20.023711501294557	28.228707061884894	30.092086925067697	21.655494511752853
100	20.076205861101236	28.280478086944484	29.942958400172337	21.700357651781946
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3260.0
1	1873.5
2	1812.5
3	3218.5
4	2096.5
5	1283.5
6	3149.0
7	8010.5
8	13864.5
9	15193.5
10	10156.5
11	5494.0
12	4820.5
13	5144.5
14	5659.0
15	6249.0
16	6846.5
17	7491.5
18	8314.5
19	9448.5
20	11195.5
21	13812.5
22	18235.0
23	25267.0
24	32800.5
25	40282.5
26	50795.5
27	64352.5
28	81300.0
29	97379.5
30	107786.0
31	118286.5
32	129654.5
33	140021.5
34	149607.5
35	156018.0
36	157974.5
37	159171.5
38	159530.0
39	157801.5
40	156996.5
41	154301.0
42	147444.5
43	141778.0
44	138627.5
45	134546.0
46	130963.5
47	127337.5
48	122384.0
49	118678.0
50	117252.5
51	113153.0
52	105188.0
53	98419.0
54	93972.0
55	90382.0
56	82700.0
57	73163.5
58	66360.5
59	60209.5
60	52984.0
61	44460.5
62	37031.5
63	29718.0
64	22535.0
65	17398.0
66	13423.0
67	10083.0
68	7511.5
69	5414.5
70	3838.5
71	2851.5
72	2161.0
73	1541.0
74	1208.0
75	904.5
76	503.5
77	374.0
78	320.0
79	164.0
80	61.5
81	30.0
82	19.5
83	10.5
84	6.0
85	4.5
86	2.0
87	2.0
88	1.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005551315274676205
2	0.004113723669680375
3	0.0010837229022276256
4	0.0
5	0.045560595481406306
6	0.0
7	0.0
8	0.0
9	2.2116793923012767E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.1058396961506384E-5
20-21	0.0
22-23	3.3175190884519156E-5
24-25	4.4233587846025534E-5
26-27	1.6587595442259576E-4
28-29	1.4375916049958297E-4
30-31	1.3270076353807662E-4
32-33	0.0011611316809581702
34-35	8.293797721129787E-4
36-37	0.0011058396961506384
38-39	8.957301538820171E-4
40-41	1.5481755746108938E-4
42-43	5.308030541523065E-4
44-45	1.4375916049958297E-4
46-47	1.6587595442259576E-4
48-49	1.7693435138410214E-4
50-51	2.101095422686213E-4
52-53	6.745622146518894E-4
54-55	7.630293903439405E-4
56-57	0.0022116793923012768
58-59	5.639782450368256E-4
60-61	0.0011058396961506384
62-63	0.0010837229022276256
64-65	0.0015813507654954127
66-67	4.3127748149874895E-4
68-69	3.7598549669121703E-4
70-71	3.317519088451915E-4
72-73	2.2116793923012765E-4
74-75	6.082118328828511E-4
76-77	7.630293903439405E-4
78-79	8.514965660359915E-4
80-81	7.519709933824341E-4
82-83	0.0015150003837263745
84-85	0.0012274820627272086
86-87	0.0019131026743406043
88-89	0.001183248474881183
90-91	0.0011390148870351576
92-93	0.0010837229022276256
94-95	7.962045812284597E-4
96-97	2.543431301146468E-4
98-99	3.649270997297107E-4
100	3.0963511492217876E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4521451.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.44163069153523
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.38455611694462	42.8871312704872
2	16.222253275367606	18.961098698071627
3	5.015024979410477	8.792587132665933
4	2.07727225351358	4.855967115424551
5	1.0654079949421407	3.1132090288108807
6	0.5862140163968114	2.0555581831478413
7	0.3761441878430574	1.538773579888399
8	0.23595132240490196	1.1031504036133313
9	0.17493569726929054	0.9201174673120276
>10	0.7882683994307353	7.9284558609590565
>50	0.04497434499074823	1.8028977314896073
>100	0.026193720644059705	2.875849507608902
>500	0.0016670599364245367	0.6200038089321099
>1k	0.0010229678150467016	1.0905691792934666
>5k	0.0	0.0
>10k+	1.1366309056060811E-4	1.4546310322951832
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27999	0.6192481130504345	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22624	0.5003703457142408	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15072	0.3333443180076484	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4767	0.10543075663100186	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4712	0.10421433296523616	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	2.2116793923012767E-5	0.0
14-15	0.0	0.0	0.0	2.2116793923012767E-5	0.0
16-17	0.0	0.0	0.0	3.317519088451915E-5	0.0
18-19	0.0	0.0	0.0	5.529198480753192E-5	0.0
20-21	0.0	0.0	0.0	8.846717569205107E-5	0.0
22-23	0.0	0.0	0.0	2.1010954226862127E-4	0.0
24-25	0.0	0.0	0.0	4.8656946630628087E-4	0.0
26-27	0.0	0.0	0.0	9.620805356510553E-4	0.0
28-29	0.0	0.0	0.0	0.002311204964954834	0.0
30-31	0.0	0.0	0.0	0.006380695046789183	0.0
32-33	0.0	0.0	0.0	0.013933580171498044	0.0
34-35	0.0	0.0	0.0	0.025025152323888945	0.0
36-37	0.0	0.0	0.0	0.04271858746229916	0.0
38-39	0.0	0.0	0.0	0.06934720734560654	0.0
40-41	0.0	0.0	0.0	0.09692684936760346	0.0
42-43	0.0	0.0	0.0	0.12383192917494848	0.0
44-45	0.0	0.0	0.0	0.15901974830646182	0.0
46-47	0.0	0.0	0.0	0.20383943119144715	0.0
48-49	0.0	0.0	0.0	0.2508486766748108	0.0
50-51	0.0	0.0	0.0	0.3018168282703937	0.0
52-53	0.0	0.0	0.0	0.35277392146901515	0.0
54-55	0.0	0.0	0.0	0.40805484787958557	0.0
56-57	0.0	0.0	0.0	0.477180887285962	0.0
58-59	0.0	0.0	0.0	0.5589577328163017	0.0
60-61	0.0	0.0	0.0	0.6459652001094339	0.0
62-63	0.0	0.0	0.0	0.7335366456476029	0.0
64-65	0.0	0.0	0.0	0.8276214869960993	0.0
66-67	0.0	0.0	0.0	0.9356730837069782	0.0
68-69	0.0	0.0	0.0	1.066151109455792	0.0
70-71	0.0	0.0	0.0	1.2100319123219516	0.0
72-73	0.0	0.0	0.0	1.361797352221665	0.0
74-75	0.0	0.0	0.0	1.5179419173181352	0.0
76-77	0.0	0.0	0.0	1.6886172160220247	0.0
78-79	0.0	0.0	0.0	1.886673105602604	0.0
80-81	0.0	0.0	0.0	2.1106719944548775	0.0
82-83	0.0	0.0	0.0	2.3456961050777725	0.0
84-85	0.0	0.0	0.0	2.5835732821167365	0.0
86-87	0.0	0.0	0.0	2.8392102446758796	0.0
88	0.0	0.0	0.0	3.0516752254973016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11800	0.0	63.72152	1
GTATCAA	22885	0.0	44.67127	1
TATCAAC	29375	0.0	34.640537	2
ATCAACG	29295	0.0	34.604427	3
TCAACGC	29620	0.0	34.208107	4
CAACGCA	30665	0.0	33.027035	5
GTGGTAT	3920	0.0	32.269028	1
AACGCAG	31850	0.0	31.932085	6
TGGTATC	3985	0.0	31.505629	2
CGCAGAG	35635	0.0	28.289806	8
ACGCAGA	35825	0.0	28.244722	7
CATGGGT	10165	0.0	28.030579	4
GTACATG	21555	0.0	27.051624	1
TACATGG	21660	0.0	26.420277	2
GCAGAGT	38750	0.0	25.736715	9
ACATGGG	21775	0.0	25.458454	3
ATGGGTG	5715	0.0	24.763788	5
TACCTGG	3580	0.0	23.77392	2
GAGTACT	26595	0.0	23.043957	12-13
CAGAGTA	38225	0.0	22.340193	10-11
>>END_MODULE