FastQCFastQC Report
Wed 25 May 2016
SRR2431388_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431388_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880705
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164890.4248970225770833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52200.046.599861
GTATCAA90600.037.1314961
TCAACGC102950.031.2551384
ATCAACG104050.030.9727273
CAACGCA112700.028.6756535
TATCAAC115600.028.6513962
AACGCAG117500.027.5961846
GTACATG203250.027.3853821
TACATGG203750.026.6002562
CATGGGT83250.025.1459164
ACATGGG210500.024.8085753
ACGCAGA134350.023.8203017
TACCTGG28750.023.7279992
CGCAGAG132450.023.6652818
GTACCTG34100.021.248631
GCAGAGT147400.020.4685259
GTGGTAT26750.019.5237731
ATGGGTG49500.019.4818045
CATGGGA53050.019.24274
CATGGGG93050.018.7058324
Produced by FastQC (version 0.11.5)