##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431388_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3880705
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.71054460465302	31.0	31.0	34.0	26.0	34.0
2	30.86722026023622	33.0	31.0	34.0	27.0	34.0
3	30.9067723519309	34.0	31.0	34.0	27.0	34.0
4	34.11667313026886	37.0	35.0	37.0	30.0	37.0
5	34.226362735636954	37.0	35.0	37.0	30.0	37.0
6	34.207573108494465	37.0	35.0	37.0	30.0	37.0
7	34.1686113734489	37.0	35.0	37.0	30.0	37.0
8	34.141828611038456	37.0	35.0	37.0	30.0	37.0
9	35.562932766082454	39.0	37.0	39.0	30.0	39.0
10-11	35.599621589376156	39.0	37.0	39.0	30.0	39.0
12-13	35.53661808872357	39.0	37.0	39.0	30.0	39.0
14-15	36.86489078659676	40.0	38.0	41.0	29.5	41.0
16-17	36.87849656183606	40.0	38.0	41.0	30.0	41.0
18-19	36.89355581009121	40.0	38.0	41.0	30.0	41.0
20-21	36.984237915533384	40.0	38.0	41.0	30.0	41.0
22-23	36.9458658929241	40.0	38.0	41.0	30.0	41.0
24-25	36.99097470691537	40.0	38.0	41.0	30.0	41.0
26-27	37.03404999864715	40.0	38.0	41.0	30.0	41.0
28-29	37.00419215065304	40.0	38.0	41.0	30.0	41.0
30-31	36.932434183994914	40.0	38.0	41.0	30.0	41.0
32-33	36.890760055196154	40.0	38.0	41.0	30.0	41.0
34-35	36.82347795052703	40.0	38.0	41.0	30.0	41.0
36-37	36.68277954649993	40.0	37.0	41.0	30.0	41.0
38-39	36.55668738025694	40.0	37.0	41.0	29.0	41.0
40-41	36.501264203282645	40.0	37.0	41.0	29.0	41.0
42-43	36.317603502456386	40.0	37.0	41.0	28.0	41.0
44-45	36.145240362253766	40.0	36.0	41.0	28.0	41.0
46-47	36.01043032129471	40.0	36.0	41.0	28.0	41.0
48-49	35.87074049689425	40.0	36.0	41.0	27.0	41.0
50-51	35.731536795504944	40.0	35.0	41.0	27.0	41.0
52-53	35.57239882959411	40.0	35.0	41.0	27.0	41.0
54-55	35.4090889413135	39.0	35.0	41.0	27.0	41.0
56-57	35.217398127402106	39.0	35.0	41.0	26.5	41.0
58-59	35.00630735910099	39.0	34.5	41.0	26.0	41.0
60-61	34.772361078721524	38.5	34.0	40.5	26.0	41.0
62-63	34.67970188406488	38.5	34.0	40.0	26.0	41.0
64-65	34.43852624716385	38.0	34.0	40.0	25.5	41.0
66-67	34.186055755333115	37.5	34.0	40.0	25.0	41.0
68-69	33.8939401474732	37.0	34.0	40.0	24.5	41.0
70-71	33.569991663885816	36.5	33.0	39.5	24.0	41.0
72-73	33.23007121128764	36.0	33.0	39.0	23.0	41.0
74-75	32.945210728462996	36.0	33.0	39.0	22.5	41.0
76-77	32.68165603930214	35.0	33.0	38.5	22.0	40.0
78-79	32.32900980620789	35.0	33.0	37.5	21.5	39.5
80-81	31.99331783786709	35.0	32.5	37.0	20.0	39.0
82-83	31.657631152071595	35.0	32.0	36.5	19.5	39.0
84-85	31.351106822085164	35.0	32.0	36.0	18.5	37.5
86-87	31.06165361706185	35.0	32.0	36.0	17.5	37.0
88-89	30.79152409162768	35.0	32.0	35.5	13.0	37.0
90-91	30.554664680773207	35.0	32.0	35.0	7.5	36.5
92-93	30.317442706930827	35.0	31.0	35.0	2.0	36.0
94-95	30.102254616107125	35.0	31.0	35.0	2.0	36.0
96-97	29.899993042501297	35.0	31.0	35.0	2.0	36.0
98-99	29.678987838549954	35.0	31.0	35.0	2.0	35.0
100	29.5044397860698	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.18469352463981537
1101	2	0.20578275742500907
1101	3	0.2871296842320632
1101	4	0.3701463611515834
1101	5	0.2892314704277865
1101	6	0.33974798075399804
1101	7	0.32264247027664794
1101	8	0.27042110224940785
1101	9	0.3286263355291865
1101	10-11	0.3138613884595287
1101	12-13	0.2594106496196744
1101	14-15	0.3126047789674118
1101	16-17	0.3021242739134209
1101	18-19	0.2597871263006155
1101	20-21	0.24197769741593333
1101	22-23	0.2929335804109314
1101	24-25	0.24319015944917055
1101	26-27	0.2488144785657198
1101	28-29	0.287370075133353
1101	30-31	0.2564825333221492
1101	32-33	0.28999533464173055
1101	34-35	0.23317248763042642
1101	36-37	0.21021848783480834
1101	38-39	0.263413312172176
1101	40-41	0.13718309714593602
1101	42-43	0.20702635977288963
1101	44-45	0.05977213225918376
1101	46-47	0.08111073213486719
1101	48-49	0.14826744700100392
1101	50-51	0.11318693183613249
1101	52-53	0.07527308530213617
1101	54-55	0.07918999022446371
1101	56-57	0.03761950172862072
1101	58-59	0.02180207286999547
1101	60-61	0.09706081383991716
1101	62-63	0.0786999297872697
1101	64-65	0.16464685208536167
1101	66-67	0.1384758406565112
1101	68-69	0.13862911323552396
1101	70-71	0.16626731066109812
1101	72-73	0.15666702551357048
1101	74-75	0.12612171940647698
1101	76-77	0.13626752702226952
1101	78-79	0.14647948628511642
1101	80-81	0.20040664001194486
1101	82-83	0.16758628903887995
1101	84-85	0.13213603360400938
1101	86-87	0.03554283611932618
1101	88-89	0.0016610224733355494
1101	90-91	0.005059341358844449
1101	92-93	-0.014545992066331337
1101	94-95	-0.09061573803243661
1101	96-97	-0.10553455515760035
1101	98-99	-0.13203341453360196
1101	100	-0.1279099665342578
1102	1	-0.04122699010385844
1102	2	0.0073914196531212895
1102	3	0.024512624082316137
1102	4	0.12245716761029968
1102	5	0.03345118850487694
1102	6	0.04049245795232537
1102	7	-0.04837402740008656
1102	8	0.04657826718573688
1102	9	0.04976772373348837
1102	10-11	-0.0032803607843945315
1102	12-13	0.023132889380185873
1102	14-15	0.06569382499803567
1102	16-17	0.07754375950821668
1102	18-19	0.009157221510626812
1102	20-21	0.003846646649748209
1102	22-23	0.009893530319203592
1102	24-25	-0.022164164733084135
1102	26-27	-0.029587113664419462
1102	28-29	-0.06487444197914272
1102	30-31	0.017434449468424873
1102	32-33	0.05679425854446407
1102	34-35	0.07723409644582091
1102	36-37	0.08065233008085215
1102	38-39	0.06961191441054382
1102	40-41	-0.015415882343539522
1102	42-43	-0.08552356736524303
1102	44-45	-0.07544524426036503
1102	46-47	-0.10837369565183508
1102	48-49	-0.061688740701654865
1102	50-51	-0.05308998473795867
1102	52-53	-0.13370883747275997
1102	54-55	-0.19274681498264812
1102	56-57	-0.10819323436650308
1102	58-59	-0.1425078616225548
1102	60-61	-0.154760291655478
1102	62-63	-0.1178698061227692
1102	64-65	-0.1295303376289496
1102	66-67	-0.06556942619938866
1102	68-69	-0.18031386320907217
1102	70-71	-0.21560998446254587
1102	72-73	-0.20236272650269171
1102	74-75	-0.17143544223335283
1102	76-77	-0.12877351901693146
1102	78-79	-0.13028964333929594
1102	80-81	-0.1431386689011731
1102	82-83	-0.15827301032678776
1102	84-85	-0.20393087646816355
1102	86-87	-0.29038595455014615
1102	88-89	-0.2534736048731645
1102	90-91	-0.24774171758146935
1102	92-93	-0.21342977263529406
1102	94-95	-0.17202703802437824
1102	96-97	-0.2237502775319946
1102	98-99	-0.2016314292442658
1102	100	-0.26317338695169923
1103	1	-0.29433645166133715
1103	2	-0.29402583098617185
1103	3	-0.23471353819389407
1103	4	-0.24627386190823586
1103	5	-0.22561762125763352
1103	6	-0.25709745756715563
1103	7	-0.25304548688815487
1103	8	-0.2069166323800502
1103	9	-0.19202824338967162
1103	10-11	-0.24796597821617894
1103	12-13	-0.19606946954581161
1103	14-15	-0.3161455010641703
1103	16-17	-0.29878903235847076
1103	18-19	-0.3400634201888053
1103	20-21	-0.32424185506117453
1103	22-23	-0.29596098555609274
1103	24-25	-0.30017168285462503
1103	26-27	-0.31419227894980395
1103	28-29	-0.39996792015243443
1103	30-31	-0.3692427571688981
1103	32-33	-0.40892450107138956
1103	34-35	-0.37612171068462885
1103	36-37	-0.4538010618965487
1103	38-39	-0.38905803888110313
1103	40-41	-0.3799583468129839
1103	42-43	-0.4221949205647135
1103	44-45	-0.5189006562884941
1103	46-47	-0.6541101801205542
1103	48-49	-0.6072440901102922
1103	50-51	-0.7342267942379692
1103	52-53	-0.7634605949698354
1103	54-55	-0.7349371941246616
1103	56-57	-0.736439362823269
1103	58-59	-0.6617607076321192
1103	60-61	-0.7091733866375804
1103	62-63	-0.7162596662662679
1103	64-65	-0.7169651909459631
1103	66-67	-0.6447336271408233
1103	68-69	-0.7049292715378996
1103	70-71	-0.7014449485315168
1103	72-73	-0.7293388007882058
1103	74-75	-0.6529505407933343
1103	76-77	-0.6514759217255133
1103	78-79	-0.6664935820315847
1103	80-81	-0.7020869016262985
1103	82-83	-0.6481703453766237
1103	84-85	-0.680480245535648
1103	86-87	-0.679400990225858
1103	88-89	-0.7449084839112601
1103	90-91	-0.696283095713369
1103	92-93	-0.7090870702079997
1103	94-95	-0.8098446248533655
1103	96-97	-0.8627776063728341
1103	98-99	-0.8720659510240658
1103	100	-0.8151450120570658
1104	1	-0.2684825229539989
1104	2	-0.207676198628441
1104	3	-0.19454767392069527
1104	4	-0.16117597720507604
1104	5	-0.14391061277343198
1104	6	-0.2174868277998243
1104	7	-0.2668620661791721
1104	8	-0.22404514176199086
1104	9	-0.2941224208028004
1104	10-11	-0.2985030793407901
1104	12-13	-0.2798115512554702
1104	14-15	-0.34244170429346354
1104	16-17	-0.3059531653547225
1104	18-19	-0.27456752239827154
1104	20-21	-0.3135521591576378
1104	22-23	-0.2587279274612513
1104	24-25	-0.2547032912921523
1104	26-27	-0.27520467541128824
1104	28-29	-0.21432348847865512
1104	30-31	-0.2521274977728183
1104	32-33	-0.29167466511508877
1104	34-35	-0.208529354255667
1104	36-37	-0.33647578817940627
1104	38-39	-0.3416073726594817
1104	40-41	-0.30310065694722965
1104	42-43	-0.39903039916232075
1104	44-45	-0.36240148945489636
1104	46-47	-0.4869311817162725
1104	48-49	-0.5198599456770765
1104	50-51	-0.6206182973061729
1104	52-53	-0.6664883661976191
1104	54-55	-0.532221488225133
1104	56-57	-0.591379596922927
1104	58-59	-0.6265795723647756
1104	60-61	-0.6455607583281022
1104	62-63	-0.6460682516403864
1104	64-65	-0.6297654639126549
1104	66-67	-0.6132296540015147
1104	68-69	-0.6604939761179622
1104	70-71	-0.6397914837089829
1104	72-73	-0.5943956555304126
1104	74-75	-0.5280747417115705
1104	76-77	-0.5438474445324459
1104	78-79	-0.6107224061093426
1104	80-81	-0.5691739839567553
1104	82-83	-0.6193385366254915
1104	84-85	-0.5650403966641484
1104	86-87	-0.5369097109568699
1104	88-89	-0.5944980175362993
1104	90-91	-0.7202342307975869
1104	92-93	-0.7129719182340324
1104	94-95	-0.659314826796237
1104	96-97	-0.7372145449042904
1104	98-99	-0.7369533500578207
1104	100	-0.6761033544917012
1105	1	-0.228843068260705
1105	2	-0.22953770036942345
1105	3	-0.24466313656235172
1105	4	-0.12327934309036692
1105	5	-0.14469777739488165
1105	6	-0.14116750670658007
1105	7	-0.14980666459663183
1105	8	-0.23855955577234766
1105	9	-0.32711415756678264
1105	10-11	-0.24923015120982228
1105	12-13	-0.15930730762122636
1105	14-15	-0.24825224568676418
1105	16-17	-0.2774706110260752
1105	18-19	-0.2854915485591363
1105	20-21	-0.24510552723156565
1105	22-23	-0.2829816356871788
1105	24-25	-0.32324131389003696
1105	26-27	-0.2132208163333047
1105	28-29	-0.2550202169634659
1105	30-31	-0.2885144034431306
1105	32-33	-0.23212510422305854
1105	34-35	-0.2966291679998889
1105	36-37	-0.3842101276538372
1105	38-39	-0.34844128098662
1105	40-41	-0.3971740520470206
1105	42-43	-0.4526990458201041
1105	44-45	-0.44777686181234344
1105	46-47	-0.43229895577449895
1105	48-49	-0.5756562001757004
1105	50-51	-0.5371231506474174
1105	52-53	-0.4349725537589819
1105	54-55	-0.4967764310641698
1105	56-57	-0.5833278312289991
1105	58-59	-0.5349724489545835
1105	60-61	-0.6024689591301708
1105	62-63	-0.45667351411171353
1105	64-65	-0.46489354752669954
1105	66-67	-0.5882024329373223
1105	68-69	-0.4271700573781061
1105	70-71	-0.4279915859121104
1105	72-73	-0.4538819761323154
1105	74-75	-0.4173998286829317
1105	76-77	-0.44039096246342524
1105	78-79	-0.47521343618728906
1105	80-81	-0.4249360607413557
1105	82-83	-0.3576963363826948
1105	84-85	-0.47228238278721335
1105	86-87	-0.4716839658792793
1105	88-89	-0.40618619175459614
1105	90-91	-0.43741096980282634
1105	92-93	-0.4600957038868998
1105	94-95	-0.4842652803187484
1105	96-97	-0.5042488103508695
1105	98-99	-0.5636046414231117
1105	100	-0.5584819253825621
1106	1	-0.4944692459776938
1106	2	-0.465274289854694
1106	3	-0.4288116055577049
1106	4	-0.3114787926414522
1106	5	-0.3809422258885391
1106	6	-0.40552348806362204
1106	7	-0.47826941782179944
1106	8	-0.5753309994603697
1106	9	-0.5341792154766125
1106	10-11	-0.5541349971653489
1106	12-13	-0.53257215316696
1106	14-15	-0.6348524150218822
1106	16-17	-0.622796625042696
1106	18-19	-0.6944564492083032
1106	20-21	-0.7277620323273482
1106	22-23	-0.6341242452775191
1106	24-25	-0.5952087772513792
1106	26-27	-0.6579120248840695
1106	28-29	-0.6616220913583675
1106	30-31	-0.6882835983548858
1106	32-33	-0.7270230055359619
1106	34-35	-0.6540684243303829
1106	36-37	-0.7144058399773172
1106	38-39	-0.73608488470731
1106	40-41	-0.7073230545138713
1106	42-43	-0.7059459772613366
1106	44-45	-0.7610067719271569
1106	46-47	-0.7622913232536703
1106	48-49	-0.7732686957026615
1106	50-51	-0.8137300414306523
1106	52-53	-0.822898809557465
1106	54-55	-0.7772841034673021
1106	56-57	-0.7127128717808802
1106	58-59	-0.8671440731008673
1106	60-61	-0.8336993061208062
1106	62-63	-0.8144628382421217
1106	64-65	-0.7906523540141279
1106	66-67	-0.740623160463656
1106	68-69	-0.7196867841609418
1106	70-71	-0.7556887087517907
1106	72-73	-0.8224409847388472
1106	74-75	-0.7023272099672226
1106	76-77	-0.7152819081569035
1106	78-79	-0.72130396461678
1106	80-81	-0.7449355338559336
1106	82-83	-0.7294362328057993
1106	84-85	-0.6964711255873617
1106	86-87	-0.7204837464900429
1106	88-89	-0.8116011103690894
1106	90-91	-0.7631094040017885
1106	92-93	-0.697622697617696
1106	94-95	-0.7347225546672611
1106	96-97	-0.7051803181725624
1106	98-99	-0.6853121563460434
1106	100	-0.7069677517986968
1107	1	-0.4316629660219071
1107	2	-0.4788863937409822
1107	3	-0.42760441125687265
1107	4	-0.37333510954174187
1107	5	-0.3317789713450452
1107	6	-0.46300257166790004
1107	7	-0.4393657463521663
1107	8	-0.4891369320195338
1107	9	-0.4476924242562461
1107	10-11	-0.3954939901668624
1107	12-13	-0.419788441443778
1107	14-15	-0.5474922923539509
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2216	72-73	0.7235308311081354
2216	74-75	0.7102429969525161
2216	76-77	0.7220374237090823
2216	78-79	0.7658994649322466
2216	80-81	0.7067893639924883
2216	82-83	0.6561009899521864
2216	84-85	0.7067034656621338
2216	86-87	0.6902806138472499
2216	88-89	0.7506135352883909
2216	90-91	0.7685641122315161
2216	92-93	0.7684716819129491
2216	94-95	0.7558416854042065
2216	96-97	0.7804709932014866
2216	98-99	0.8068686300200234
2216	100	0.8065377176857496
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97922.0
3	18260.0
4	12594.0
5	11162.0
6	8165.0
7	6950.0
8	7759.0
9	8192.0
10	8423.0
11	9040.0
12	10306.0
13	11476.0
14	12404.0
15	12669.0
16	12436.0
17	12366.0
18	12192.0
19	11630.0
20	11371.0
21	12091.0
22	14138.0
23	19769.0
24	30580.0
25	41505.0
26	46884.0
27	37475.0
28	35637.0
29	43301.0
30	51574.0
31	59057.0
32	75250.0
33	100810.0
34	143595.0
35	223112.0
36	374857.0
37	690306.0
38	1060702.0
39	522662.0
40	2083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.91461296009954	21.90000232324614	14.091313898949117	22.094070817705198
2	14.641139605963794	22.54416561631817	40.70910719904515	22.10558757867289
3	17.234049254450348	29.284232059848435	30.629133516353868	22.852585169347346
4	11.708334118488878	19.078195555098944	39.84001412505579	29.373456201356383
5	11.695182350292662	40.6788670695488	35.31092903849411	12.31502154166443
6	27.18140700264921	41.09998011739249	18.921150633058616	12.797462246899693
7	24.664168269426664	35.57998249471463	23.359369479632957	16.396479756225748
8	24.113118017054187	37.563384628951894	21.188548336315456	17.134949017678462
9	25.02897483862401	18.600699005895528	21.749835304309933	34.62049085117053
10-11	22.243583123065992	29.539482616632128	29.40002215172695	18.816912108574925
12-13	22.82919742136326	27.78755979775459	29.005666265156865	20.377576515725284
14-15	20.850249513659815	29.18759270376261	26.484858987133386	23.477298795444185
16-17	20.001469783381005	32.2570651133377	27.211530837409082	20.52993426587221
18-19	20.08503005345903	31.159411881050758	29.037064428561372	19.71849363692884
20-21	21.767743346309604	30.067230587686776	28.390940026530174	19.774086039473442
22-23	21.36713687807936	30.082274426102646	28.455184864424677	20.09540383139332
24-25	20.88708873659454	30.295670888448996	28.37861098285728	20.438629392099187
26-27	20.53401068898158	30.291116798720935	28.53407515811054	20.640797354186947
28-29	20.624006551986277	30.146718282125313	28.777706949210756	20.45156821667765
30-31	20.860858325889033	30.45279816220387	28.419950238389475	20.26639327351762
32-33	20.46833949313894	30.346446515936243	28.750127648776587	20.435086342148235
34-35	20.87043313937596	30.008177257990816	28.668883715316923	20.452505887316303
36-37	20.89395437963499	30.215039065774153	28.283955759413114	20.607050795177738
38-39	20.710527116613275	30.287722825489883	28.498813437019837	20.50293662087701
40-41	20.657197815134328	29.989630630205475	28.717945147297403	20.635226407362794
42-43	20.54975307209263	30.142908720487927	28.658014519103066	20.649323688316375
44-45	20.740082772466245	30.05932933746564	28.591525629404615	20.609062260663503
46-47	20.763227806422805	30.158190433786146	28.45033308305201	20.628248676739037
48-49	20.72275164628956	30.034534459216104	28.51138698388353	20.7313269106108
50-51	20.67285957259149	29.96363687942531	28.484418660728217	20.879084887254983
52-53	20.687552457002504	29.977636827913695	28.528318109707847	20.806492605375958
54-55	20.77232909292021	30.234535842524537	28.385782086590666	20.607352977964585
56-57	20.76121676367023	30.01440128671609	28.304259771412937	20.92012217820074
58-59	20.717899161581858	29.719956038463636	28.577332626173103	20.984812173781403
60-61	20.869475179708292	30.07641188687807	28.33082340677689	20.723289526636748
62-63	20.67388164522966	30.02240100506685	28.44337125456201	20.86034609514148
64-65	20.784353367750448	29.863349715826047	28.45629572400049	20.896001192423007
66-67	20.637262625686958	29.909105420640476	28.37255972411083	21.08107222956174
68-69	21.01671138594521	30.06587336686527	28.191132044575728	20.72628320261379
70-71	20.966762892127345	29.863539489613967	28.30039362616989	20.8693039920888
72-73	20.82668710944571	29.953338480127854	28.40721149457653	20.81276291584991
74-75	20.821709888090012	29.986812579262484	28.384904554946466	20.806572977701034
76-77	20.80553424093021	29.879637660968044	28.252340694003053	21.062487404098693
78-79	20.873084270439435	29.911542238368433	28.3953334488599	20.820040042332234
80-81	20.80657178173457	29.896155079070635	28.330641382357015	20.966631756837785
82-83	20.709197891289456	29.931060592482	28.566534078153506	20.793207438075033
84-85	20.752309267282094	29.833761640469845	28.34409355883578	21.069835533412277
86-87	20.932433917040992	29.820591258672184	28.281763120012087	20.96521170427473
88-89	20.785172764261713	29.77468861273061	28.429940733454767	21.01019788955291
90-91	20.785007399514058	29.7550984566691	28.2773619226951	21.182532221121747
92-93	20.892049469053475	29.789568280393404	28.27638358114276	21.041998669410365
94-95	20.736070122987794	29.684902203759606	28.45172359418992	21.12730407906268
96-97	20.80683385742417	29.82075358456085	28.252955045860794	21.119457512154185
98-99	20.671271131070696	29.828346739560295	28.2875278828073	21.212854246561704
100	20.72526712970008	29.828593387717838	28.13000183115701	21.31613765142508
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15617.0
1	11465.0
2	6538.5
3	4907.0
4	3418.5
5	2505.0
6	2120.5
7	2209.0
8	2520.5
9	2543.0
10	2104.5
11	1611.0
12	1508.0
13	1551.0
14	1619.0
15	1753.0
16	2069.0
17	2609.5
18	3320.0
19	4322.5
20	5892.5
21	8342.5
22	12128.5
23	17987.0
24	24680.5
25	31719.0
26	41144.0
27	52655.5
28	66320.5
29	79424.5
30	88844.5
31	97615.5
32	106212.5
33	115357.5
34	123405.5
35	129298.0
36	132970.0
37	134636.5
38	136110.5
39	136298.5
40	136620.0
41	135773.0
42	133378.0
43	131233.5
44	129289.0
45	126484.5
46	123644.0
47	119756.0
48	114647.5
49	110838.0
50	109104.5
51	104588.5
52	95398.0
53	87363.0
54	82143.0
55	78266.0
56	70727.0
57	61820.0
58	55081.0
59	49063.0
60	42621.5
61	35266.0
62	29068.5
63	23446.0
64	18476.0
65	14915.0
66	11922.5
67	9222.0
68	7067.5
69	5415.5
70	4126.0
71	3130.0
72	2372.0
73	1854.5
74	1515.5
75	1163.5
76	854.5
77	692.5
78	530.0
79	373.5
80	276.5
81	232.0
82	187.5
83	157.0
84	135.5
85	107.0
86	87.0
87	73.5
88	66.0
89	64.5
90	70.5
91	61.5
92	47.5
93	40.5
94	36.0
95	39.5
96	40.0
97	32.5
98	35.0
99	39.5
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17561242093897886
2	0.16991757940889607
3	0.18519830803938975
4	0.17362824538324867
5	0.18762054832820327
6	0.07586250436454202
7	0.07779514289285065
8	0.06689506159318989
9	0.060298322083229716
10-11	0.07481887955925534
12-13	0.07323411596604225
14-15	0.07114686635546892
16-17	0.06654718665809435
18-19	0.07168800514339534
20-21	0.07362064367170398
22-23	0.07134013020829978
24-25	0.07115975061232431
26-27	0.07421331948705197
28-29	0.07207453284905706
30-31	0.07774360586542908
32-33	0.07413601394591962
34-35	0.07413601394591962
36-37	0.08426303983425693
38-39	0.08037199426392885
40-41	0.07570789328227731
42-43	0.0787228093864388
44-45	0.08888848804534227
46-47	0.07907068432153437
48-49	0.08435322963224465
50-51	0.08117081818896309
52-53	0.08051372108933814
54-55	0.08178926251802185
56-57	0.07541155537460333
58-59	0.08309057246041635
60-61	0.07992104527399016
62-63	0.07096648675949344
64-65	0.07438081482617205
66-67	0.07514098598064012
68-69	0.07240952352729724
70-71	0.06854424647067994
72-73	0.06604470064073409
74-75	0.07130147743773362
76-77	0.076158842272216
78-79	0.07238375501358645
80-81	0.07977931844858087
82-83	0.08167330420632334
84-85	0.08324518354268103
86-87	0.07934125371549758
88-89	0.0808615960244337
90-91	0.07950874905461766
92-93	0.07021919986188077
94-95	0.07005170452276069
96-97	0.07007747303647147
98-99	0.07966336013688236
100	0.08696873377388901
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3880705.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.36961718638854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.90731015058616	45.82495255439351
2	14.41653318322662	17.40641178852517
3	4.608035473023402	8.345560124631648
4	1.8776710163967643	4.53417121847399
5	0.9803167137361256	2.959067236483417
6	0.5359217958335648	1.941203619378851
7	0.36088269776086535	1.5250445219110218
8	0.23696218287029389	1.1444253014024508
9	0.1629337662543452	0.8852624185960197
>10	0.8246522043424919	8.944742818849448
>50	0.05845642731481947	2.4449859181855462
>100	0.02917155518374568	3.0702908961569526
>500	8.539507476109211E-4	0.32509119168147926
>1k	2.561851914846164E-4	0.22376437771711696
>5k	0.0	0.0
>10k+	4.2697531914101965E-5	0.42502601361335024
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	16489	0.4248970225770833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5768513710781932E-5	0.0
5	0.0	0.0	0.0	2.5768513710781932E-5	0.0
6	0.0	0.0	0.0	2.5768513710781932E-5	0.0
7	0.0	0.0	0.0	2.5768513710781932E-5	0.0
8	0.0	0.0	0.0	2.5768513710781932E-5	0.0
9	0.0	0.0	0.0	7.73055411323458E-5	0.0
10-11	0.0	0.0	0.0	7.73055411323458E-5	0.0
12-13	0.0	0.0	0.0	7.73055411323458E-5	0.0
14-15	0.0	0.0	0.0	7.73055411323458E-5	0.0
16-17	0.0	0.0	0.0	1.1595831169851868E-4	0.0
18-19	0.0	0.0	0.0	1.2884256855390965E-4	0.0
20-21	0.0	0.0	0.0	1.932638528308645E-4	0.0
22-23	0.0	0.0	0.0	3.73643448806338E-4	0.0
24-25	0.0	0.0	0.0	5.669073016372025E-4	0.0
26-27	0.0	0.0	0.0	0.0010565090621420593	0.0
28-29	0.0	0.0	0.0	0.0028345365081860127	0.0
30-31	0.0	0.0	0.0	0.006777119105935648	0.0
32-33	0.0	0.0	0.0	0.01453344173288101	0.0
34-35	0.0	0.0	0.0	0.026876559800345555	0.0
36-37	0.0	0.0	0.0	0.04616429231286583	0.0
38-39	0.0	0.0	0.0	0.0731568104249099	0.0
40-41	0.0	0.0	0.0	0.10038124516035102	0.0
42-43	0.0	0.0	0.0	0.12759279563893675	0.0
44-45	0.0	0.0	0.0	0.16198087718597523	0.0
46-47	0.0	2.5768513710781932E-5	0.0	0.20658617441933874	0.0
48-49	0.0	2.5768513710781932E-5	0.0	0.2556107717541014	0.0
50-51	0.0	2.5768513710781932E-5	0.0	0.3060139845723908	0.0
52-53	0.0	2.5768513710781932E-5	0.0	0.3573706323979792	0.0
54-55	0.0	2.5768513710781932E-5	0.0	0.4157878529803219	0.0
56-57	0.0	2.5768513710781932E-5	0.0	0.4856462936502517	0.0
58-59	0.0	2.5768513710781932E-5	0.0	0.5658121398044943	0.0
60-61	0.0	2.5768513710781932E-5	0.0	0.651221878498881	0.0
62-63	0.0	2.5768513710781932E-5	0.0	0.7379973484199391	0.0
64-65	0.0	2.5768513710781932E-5	0.0	0.8278908085000019	0.0
66-67	0.0	2.5768513710781932E-5	0.0	0.9384119638055457	0.0
68-69	0.0	2.5768513710781932E-5	0.0	1.0639561626044753	0.0
70-71	0.0	2.5768513710781932E-5	0.0	1.2055670297020773	0.0
72-73	0.0	2.5768513710781932E-5	0.0	1.3544317334092646	0.0
74-75	0.0	2.5768513710781932E-5	0.0	1.5070328716045152	0.0
76-77	0.0	2.5768513710781932E-5	0.0	1.6721575074632056	0.0
78-79	0.0	2.5768513710781932E-5	0.0	1.8691191420115674	0.0
80-81	0.0	2.5768513710781932E-5	0.0	2.086901735638241	0.0
82-83	0.0	2.5768513710781932E-5	0.0	2.3252733717198293	0.0
84-85	0.0	2.5768513710781932E-5	0.0	2.558993791076621	0.0
86-87	0.0	2.5768513710781932E-5	0.0	2.8095152813728435	0.0
88	0.0	2.5768513710781932E-5	0.0	3.0114888918379523	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5220	0.0	46.59986	1
GTATCAA	9060	0.0	37.131496	1
TCAACGC	10295	0.0	31.255138	4
ATCAACG	10405	0.0	30.972727	3
CAACGCA	11270	0.0	28.675653	5
TATCAAC	11560	0.0	28.651396	2
AACGCAG	11750	0.0	27.596184	6
GTACATG	20325	0.0	27.385382	1
TACATGG	20375	0.0	26.600256	2
CATGGGT	8325	0.0	25.145916	4
ACATGGG	21050	0.0	24.808575	3
ACGCAGA	13435	0.0	23.820301	7
TACCTGG	2875	0.0	23.727999	2
CGCAGAG	13245	0.0	23.665281	8
GTACCTG	3410	0.0	21.24863	1
GCAGAGT	14740	0.0	20.468525	9
GTGGTAT	2675	0.0	19.523773	1
ATGGGTG	4950	0.0	19.481804	5
CATGGGA	5305	0.0	19.2427	4
CATGGGG	9305	0.0	18.705832	4
>>END_MODULE