FastQCFastQC Report
Wed 25 May 2016
SRR2431388_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431388_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3880705
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT266630.6870658810705786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT214150.5518327211163951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144710.3728961619087254No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46890.12082856078985647No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45210.11649945048644511No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39510.1018113976712994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100900.062.311671
GTATCAA200550.045.770561
GTGGTAT33500.035.7951771
ATCAACG259000.035.0382043
TCAACGC259850.034.8874054
TATCAAC264200.034.5105362
CAACGCA269300.033.680635
AACGCAG278700.032.5970086
TGGTATC36250.031.1322122
ACGCAGA316800.028.528377
CGCAGAG317100.028.4569178
TATAACG3150.028.3628432
GTACATG193150.026.7810171
GCAGAGT341350.026.2150049
CATGGGT81300.026.0259344
TACATGG194750.025.7145462
ACATGGG195300.025.0389123
TACCTGG30200.024.7569372
GAGTACT236200.022.89230712-13
GTACCTG33550.022.7065731
Produced by FastQC (version 0.11.5)