##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431388_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3880705
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.609717564205475	34.0	31.0	34.0	31.0	34.0
2	32.755699544283836	34.0	31.0	34.0	31.0	34.0
3	32.83886742228538	34.0	31.0	34.0	31.0	34.0
4	36.30177583712238	37.0	37.0	37.0	35.0	37.0
5	36.215470384891404	37.0	37.0	37.0	35.0	37.0
6	36.265893181780115	37.0	37.0	37.0	35.0	37.0
7	36.21921016928625	37.0	37.0	37.0	35.0	37.0
8	36.235889612840964	37.0	37.0	37.0	35.0	37.0
9	38.030597533180185	39.0	39.0	39.0	35.0	39.0
10-11	38.006208279165776	39.0	39.0	39.0	35.0	39.0
12-13	37.99443928358378	39.0	39.0	39.0	35.0	39.0
14-15	39.45591187683681	41.0	39.0	41.0	36.0	41.0
16-17	39.4846938893835	41.0	39.0	41.0	36.0	41.0
18-19	39.553314539497336	41.0	39.5	41.0	36.5	41.0
20-21	39.55231613843361	41.0	40.0	41.0	37.0	41.0
22-23	39.49766936677743	41.0	39.5	41.0	36.0	41.0
24-25	39.45189984809461	41.0	39.0	41.0	36.0	41.0
26-27	39.33448754800996	41.0	39.0	41.0	36.0	41.0
28-29	39.26059066587128	41.0	39.0	41.0	36.0	41.0
30-31	39.089637578738916	41.0	39.0	41.0	35.0	41.0
32-33	38.87944626040887	41.0	38.0	41.0	35.0	41.0
34-35	38.75708099430388	41.0	38.0	41.0	35.0	41.0
36-37	38.623409277437986	41.0	38.0	41.0	35.0	41.0
38-39	38.48310319903213	41.0	38.0	41.0	35.0	41.0
40-41	38.351936052856374	41.0	38.0	41.0	35.0	41.0
42-43	38.185630832542024	40.0	38.0	41.0	34.0	41.0
44-45	37.953035208808714	40.0	38.0	41.0	33.5	41.0
46-47	37.597649267336735	40.0	38.0	41.0	33.0	41.0
48-49	37.2750174259574	40.0	38.0	41.0	32.5	41.0
50-51	37.11264950569549	40.0	37.0	41.0	32.0	41.0
52-53	37.057150698133455	40.0	37.0	41.0	32.0	41.0
54-55	36.91667093994519	40.0	37.0	41.0	31.5	41.0
56-57	36.76944743287625	40.0	36.5	41.0	31.0	41.0
58-59	36.5919464375674	40.0	36.0	41.0	31.0	41.0
60-61	36.44025673170211	40.0	35.5	41.0	31.0	41.0
62-63	36.282047334182835	39.0	35.0	41.0	31.0	41.0
64-65	36.05297929113396	39.0	35.0	41.0	31.0	41.0
66-67	35.78664082943692	39.0	35.0	41.0	30.0	41.0
68-69	35.491639663411675	38.0	35.0	41.0	30.0	41.0
70-71	35.18957624967628	37.0	35.0	40.0	29.5	41.0
72-73	34.865595040076485	37.0	35.0	39.5	29.0	41.0
74-75	34.52149145580506	36.0	35.0	39.0	29.0	41.0
76-77	34.184866409582796	36.0	35.0	39.0	29.0	41.0
78-79	33.85895668441688	35.5	35.0	38.0	29.0	40.0
80-81	33.58806453981944	35.0	34.5	37.0	29.0	39.0
82-83	33.38014123722365	35.0	35.0	37.0	29.0	39.0
84-85	33.113979805215806	35.0	34.0	36.5	29.0	38.5
86-87	32.868880654417175	35.0	34.0	36.0	29.0	37.0
88-89	32.63194690140065	35.0	34.0	36.0	29.0	37.0
90-91	32.44862750453848	35.0	34.0	35.5	29.0	36.5
92-93	32.287066654125994	35.0	34.0	35.0	29.0	36.0
94-95	32.14511126200007	35.0	34.0	35.0	28.0	36.0
96-97	32.00357009873206	35.0	34.0	35.0	27.0	36.0
98-99	31.888990789044776	35.0	34.0	35.0	27.0	36.0
100	31.78694025956624	35.0	34.0	35.0	27.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.020264469373373117
1101	2	0.052194658262045834
1101	3	0.0355677106911898
1101	4	0.024275841044229196
1101	5	0.04987814857781814
1101	6	0.03695943957357173
1101	7	0.025430982510251
1101	8	0.05058565195349729
1101	9	0.02307998650158538
1101	10-11	0.07236233629980404
1101	12-13	0.0736706624209944
1101	14-15	0.11899089888622427
1101	16-17	0.020775772191008457
1101	18-19	-0.038815527084224755
1101	20-21	0.04564131645397396
1101	22-23	0.06713667850730332
1101	24-25	0.06376938558313583
1101	26-27	0.09053524101322807
1101	28-29	0.01480753902826848
1101	30-31	0.034571206910079866
1101	32-33	0.027985106177979446
1101	34-35	0.06301903876054382
1101	36-37	0.0401561209325223
1101	38-39	-0.016823558692756535
1101	40-41	-0.03326246445890746
1101	42-43	-0.04342122004577931
1101	44-45	-0.020898874157786906
1101	46-47	-0.002592169067092698
1101	48-49	0.006698693163528446
1101	50-51	0.07643972080663985
1101	52-53	0.11193832640711321
1101	54-55	0.0648798185938233
1101	56-57	0.03972952603088231
1101	58-59	0.014390851541243421
1101	60-61	0.01749090106859086
1101	62-63	0.03494923102018532
1101	64-65	0.033941762330094605
1101	66-67	0.029058619215774684
1101	68-69	0.026610203354955786
1101	70-71	0.05549188196044952
1101	72-73	0.01055709351645362
1101	74-75	-0.00871915007721924
1101	76-77	-0.013120789238413977
1101	78-79	-0.0375660992867779
1101	80-81	-0.07078638602057197
1101	82-83	-0.1358884087423391
1101	84-85	-0.052166580372620786
1101	86-87	-0.031060227599198242
1101	88-89	-0.010674504983001043
1101	90-91	-0.04448739398979029
1101	92-93	-0.08609654962817359
1101	94-95	-0.08151432455056096
1101	96-97	-0.029766326855373393
1101	98-99	-0.05292702982946906
1101	100	-0.0711378898609567
1102	1	-0.015090720535013702
1102	2	0.007339171967736036
1102	3	0.010131750457361477
1102	4	0.010537898770294873
1102	5	0.015985364551653447
1102	6	-0.05997550859269296
1102	7	-0.03799800079667648
1102	8	-0.012812982090139258
1102	9	0.053723666012231774
1102	10-11	0.028218418895690434
1102	12-13	0.03630455806030852
1102	14-15	0.039254733257536145
1102	16-17	0.010019542610777421
1102	18-19	0.05044540658454366
1102	20-21	0.04892242478764075
1102	22-23	0.04120224029060893
1102	24-25	0.050871185048627865
1102	26-27	0.09363880773238975
1102	28-29	0.009387690061707588
1102	30-31	0.0517375601725476
1102	32-33	0.07358326699371531
1102	34-35	0.037891345732212756
1102	36-37	-0.008089028251433206
1102	38-39	0.0010374397274119929
1102	40-41	0.0017854824922238777
1102	42-43	0.06604953386931811
1102	44-45	0.017535852030242438
1102	46-47	0.03571200486425852
1102	48-49	0.015281641308611427
1102	50-51	0.01156013410876966
1102	52-53	0.07246164036135383
1102	54-55	0.059452784131906355
1102	56-57	-0.02019643374250535
1102	58-59	-4.469833146458768E-5
1102	60-61	-0.03328163580426491
1102	62-63	-0.06060868629423766
1102	64-65	-0.07964542951182096
1102	66-67	-0.02932858878720168
1102	68-69	-0.0696350633181595
1102	70-71	-0.049796693048349994
1102	72-73	-0.029168642428757607
1102	74-75	-0.029430111351842925
1102	76-77	-0.06513027131893523
1102	78-79	-0.015158778073804058
1102	80-81	-0.036130978268765546
1102	82-83	-0.022659168024496523
1102	84-85	-0.01635763273537094
1102	86-87	-0.023958263623676146
1102	88-89	-0.014084495260831886
1102	90-91	-0.021889436542487317
1102	92-93	-0.0032334644203970697
1102	94-95	0.014599885620576458
1102	96-97	0.023323358936465866
1102	98-99	0.016885777616366227
1102	100	0.025073088132550936
1103	1	-0.08741509895948951
1103	2	-0.0883592415141976
1103	3	-0.04503051837676253
1103	4	-0.0014576772032626195
1103	5	-0.07209573100008981
1103	6	-0.05672604679691773
1103	7	-0.08018152413505675
1103	8	-0.07173951265066592
1103	9	-0.037565000011035465
1103	10-11	-0.028566122203287136
1103	12-13	-0.006004213718213691
1103	14-15	-0.0511313780379723
1103	16-17	-0.026714467905740946
1103	18-19	-0.0588235264500554
1103	20-21	-0.14208560718821417
1103	22-23	-0.12682144996248468
1103	24-25	-0.1284194747368943
1103	26-27	-0.1215913458508453
1103	28-29	-0.07781444418306194
1103	30-31	-0.09943243709081173
1103	32-33	-0.09342907402020018
1103	34-35	-0.12431380033801531
1103	36-37	-0.14293488425384027
1103	38-39	-0.11599457980699412
1103	40-41	-0.15647284834938802
1103	42-43	-0.13740239635618678
1103	44-45	-0.06333383644771828
1103	46-47	0.056122252371061165
1103	48-49	0.06704702492817205
1103	50-51	0.060641210558820546
1103	52-53	0.09355756279413185
1103	54-55	0.1255310526339244
1103	56-57	0.1385925835815911
1103	58-59	0.06430155776617852
1103	60-61	0.04024978959201775
1103	62-63	0.06728855267977707
1103	64-65	0.0028271458552140416
1103	66-67	0.03249137459430074
1103	68-69	0.01424905109452368
1103	70-71	-0.008500972639559734
1103	72-73	0.018091532576747227
1103	74-75	0.04819895695295884
1103	76-77	0.03555396625233698
1103	78-79	0.0032205912717060414
1103	80-81	0.02633153939792976
1103	82-83	-0.013293714731055672
1103	84-85	-0.02289825852556504
1103	86-87	-0.008946890554675235
1103	88-89	0.012113137983362776
1103	90-91	-8.376276599690868E-4
1103	92-93	0.04796520428655526
1103	94-95	0.08898663162165832
1103	96-97	0.02616941579027099
1103	98-99	0.007074271554230904
1103	100	0.05972373812892684
1104	1	-0.11838120637040106
1104	2	-0.10403730065148409
1104	3	-0.12227815617583104
1104	4	-0.07580992091892824
1104	5	-0.04898713894906592
1104	6	-0.06493439887562857
1104	7	-0.07352437379639554
1104	8	-0.12182505702622137
1104	9	-0.10651785089132915
1104	10-11	-0.1456468957658501
1104	12-13	-0.11172388743943884
1104	14-15	-0.17141716302144516
1104	16-17	-0.07718922078296231
1104	18-19	-0.08603838697467836
1104	20-21	-0.09843741316033316
1104	22-23	-0.08844832985732154
1104	24-25	-0.04385962882060568
1104	26-27	-0.06084099452641567
1104	28-29	-0.03753215875652671
1104	30-31	-0.06262415887974981
1104	32-33	-0.05370326597401487
1104	34-35	-0.0898985903988958
1104	36-37	-0.07577720036017865
1104	38-39	-0.22871380791838192
1104	40-41	-0.27310620283360976
1104	42-43	-0.2819025226259484
1104	44-45	-0.2829578269583166
1104	46-47	-0.3109725546602462
1104	48-49	-0.34751139624491145
1104	50-51	-0.4818343392683957
1104	52-53	-0.42758058550414546
1104	54-55	-0.3458644115853531
1104	56-57	-0.3741645632140944
1104	58-59	-0.3220570012621451
1104	60-61	-0.3564723287846334
1104	62-63	-0.3489796547233084
1104	64-65	-0.3207741027585129
1104	66-67	-0.268653838265422
1104	68-69	-0.31350443661578
1104	70-71	-0.3446434697398928
1104	72-73	-0.2918059866017444
1104	74-75	-0.410064972067147
1104	76-77	-0.32649143510266043
1104	78-79	-0.2873597008421882
1104	80-81	-0.3090509006296003
1104	82-83	-0.35229051239416975
1104	84-85	-0.33297237600759644
1104	86-87	-0.3306596307354468
1104	88-89	-0.30235394292864726
1104	90-91	-0.2862120692396175
1104	92-93	-0.30082420136331933
1104	94-95	-0.3350418794782861
1104	96-97	-0.3317515302190728
1104	98-99	-0.2973670601451772
1104	100	-0.2738837198227415
1105	1	-0.15201686521417912
1105	2	-0.1414963927795725
1105	3	-0.09497234819978928
1105	4	-0.008149971054130845
1105	5	-0.08449497563301378
1105	6	-0.023355861707941017
1105	7	0.004392128099318882
1105	8	-0.018577943810193176
1105	9	-0.008630549179201807
1105	10-11	-0.07938566993720997
1105	12-13	-0.06870929787056923
1105	14-15	-0.11451508295044732
1105	16-17	-0.11906624477268224
1105	18-19	-0.13576847082869392
1105	20-21	-0.04822613897294303
1105	22-23	-0.13023690523568376
1105	24-25	-0.13595595928976678
1105	26-27	-0.06548999488276763
1105	28-29	-0.07617687011798324
1105	30-31	-0.07494292406365588
1105	32-33	-0.15261314384394353
1105	34-35	-0.12222769420120017
1105	36-37	-0.19085052491278276
1105	38-39	-0.11505844754337602
1105	40-41	-0.21389641100882528
1105	42-43	-0.23800618664002116
1105	44-45	-0.28962606147857883
1105	46-47	-0.2554003637504749
1105	48-49	-0.3789790522907168
1105	50-51	-0.3745750183382839
1105	52-53	-0.35279899691229133
1105	54-55	-0.2972097675787211
1105	56-57	-0.2205975530704265
1105	58-59	-0.3145041560966533
1105	60-61	-0.29253687180045773
1105	62-63	-0.3149079171399123
1105	64-65	-0.3659779744727203
1105	66-67	-0.38657638492790625
1105	68-69	-0.301941454075056
1105	70-71	-0.30462746244033667
1105	72-73	-0.27616560402306334
1105	74-75	-0.2967554516777966
1105	76-77	-0.31931914312299625
1105	78-79	-0.3171091624451279
1105	80-81	-0.2760524235895403
1105	82-83	-0.3255953454118483
1105	84-85	-0.3809669710844972
1105	86-87	-0.37063250338134424
1105	88-89	-0.3360204389246704
1105	90-91	-0.3047901381060001
1105	92-93	-0.30987614308275013
1105	94-95	-0.32646789026907896
1105	96-97	-0.2928381262351891
1105	98-99	-0.33422088170737396
1105	100	-0.3468401568424433
1106	1	-0.19390645198232903
1106	2	-0.15606770196545483
1106	3	-0.1502667783622229
1106	4	-0.08047085013319588
1106	5	-0.09654674798046159
1106	6	-0.16500333730473216
1106	7	-0.10757779406068124
1106	8	-0.04191274803201139
1106	9	-0.14289870166277296
1106	10-11	-0.1440185954465889
1106	12-13	-0.11259217636273888
1106	14-15	-0.2327608874422964
1106	16-17	-0.2528677926449774
1106	18-19	-0.21565875425646652
1106	20-21	-0.1636078272162962
1106	22-23	-0.16788460695413931
1106	24-25	-0.10750917727910547
1106	26-27	-0.14131246669256825
1106	28-29	-0.12736195984583532
1106	30-31	-0.2211625943065556
1106	32-33	-0.23111266928116692
1106	34-35	-0.29048096290325987
1106	36-37	-0.24080246491224244
1106	38-39	-0.2303747324742318
1106	40-41	-0.28350017121448445
1106	42-43	-0.3665390631929668
1106	44-45	-0.35824566190916585
1106	46-47	-0.4392309634997602
1106	48-49	-0.36569733177562114
1106	50-51	-0.48089491755681024
1106	52-53	-0.4018071168021464
1106	54-55	-0.4500551135338853
1106	56-57	-0.3973647780161116
1106	58-59	-0.3278822893269506
1106	60-61	-0.4217191163470062
1106	62-63	-0.46244132496898516
1106	64-65	-0.48415773533991313
1106	66-67	-0.43872441265671114
1106	68-69	-0.44659400606657584
1106	70-71	-0.4220018020955649
1106	72-73	-0.46223116759654204
1106	74-75	-0.47191146830688524
1106	76-77	-0.4294880501503968
1106	78-79	-0.40998698869147887
1106	80-81	-0.460867731952888
1106	82-83	-0.43021933216360253
1106	84-85	-0.38678704263846697
1106	86-87	-0.47048433514974874
1106	88-89	-0.45301809208916666
1106	90-91	-0.46175159350990214
1106	92-93	-0.40688343385983927
1106	94-95	-0.465506002380053
1106	96-97	-0.49072284589921367
1106	98-99	-0.4355102142533056
1106	100	-0.3162707400533833
1107	1	-0.22668882646154742
1107	2	-0.17747453997860418
1107	3	-0.1622783123603071
1107	4	-0.14565073812735818
1107	5	-0.1898634320886572
1107	6	-0.17425532676882938
1107	7	-0.1263851624541843
1107	8	-0.12611286999726445
1107	9	-0.14634300114948928
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2216	32-33	-0.0871442795834767
2216	34-35	-0.015228668761338326
2216	36-37	-0.011028606853230372
2216	38-39	0.006012379898422182
2216	40-41	-0.03166484250662194
2216	42-43	0.019949252171834075
2216	44-45	-0.007899472699641308
2216	46-47	0.05758052756271326
2216	48-49	0.010535491566265875
2216	50-51	0.05554168836074069
2216	52-53	0.05254814060830881
2216	54-55	0.1137194287229164
2216	56-57	0.14416195289958011
2216	58-59	0.03495790859865622
2216	60-61	0.0023386470668711468
2216	62-63	-0.05843709836292277
2216	64-65	0.0014894781325267559
2216	66-67	0.02343537424026465
2216	68-69	0.04466129957769738
2216	70-71	0.04079744716885614
2216	72-73	0.044479753371931
2216	74-75	0.08674722323617345
2216	76-77	0.028088613240981886
2216	78-79	0.05482675864526243
2216	80-81	0.07510448306005912
2216	82-83	0.07607606917121501
2216	84-85	0.050546859544319034
2216	86-87	0.07898974471197562
2216	88-89	0.07846699655755174
2216	90-91	0.048422927012843786
2216	92-93	0.11717527055365906
2216	94-95	0.0871064250681215
2216	96-97	0.07336791776448592
2216	98-99	0.10406086901028289
2216	100	0.1681389450790327
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56.0
3	229.0
4	67.0
5	101.0
6	299.0
7	627.0
8	1262.0
9	2299.0
10	3308.0
11	4591.0
12	6840.0
13	10329.0
14	11726.0
15	15620.0
16	20469.0
17	31249.0
18	36631.0
19	18889.0
20	11707.0
21	10371.0
22	10385.0
23	10418.0
24	11283.0
25	13112.0
26	16185.0
27	20352.0
28	25375.0
29	32436.0
30	41015.0
31	51862.0
32	66080.0
33	84588.0
34	118804.0
35	190342.0
36	311938.0
37	661411.0
38	1226862.0
39	792832.0
40	8755.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.376808886495155	21.385416035840787	14.649832044623173	22.58794303304089
2	14.240322924378765	22.19360361723133	40.714416587188836	22.851656871201072
3	16.47358984256826	28.598029307340546	32.020457313254994	22.907923536836204
4	10.959220038627002	18.588658504060472	40.208158053755696	30.24396340355683
5	11.087322145616312	39.553072222439916	35.819496490862626	13.54010914108114
6	27.088376983048185	40.412090071262824	18.662536832869286	13.836996112819708
7	24.049959994382462	35.368444651165184	23.771325055627777	16.81027029882457
8	24.814279879557965	35.50058558947408	21.19096916668492	18.494165364283035
9	25.751390920665663	15.957727246841616	22.4129236378048	35.87795819468792
10-11	22.65974867968578	27.850802882465942	29.707385642557217	19.78206279529106
12-13	24.128618382484625	26.150596347828554	29.908496007813014	19.812289261873808
14-15	21.470609077474325	27.162397038682407	28.599597238130702	22.76739664571257
16-17	19.225089255689365	30.534889923351553	29.184864605786835	21.055156215172243
18-19	19.149293091816894	28.227702101320844	33.158034293795296	19.464970513066966
20-21	20.637966637243316	27.23416585828681	33.06483397537741	19.06303352909246
22-23	20.155579998425544	27.322745135580405	33.161195344814324	19.360479521179723
24-25	19.834380544661553	27.469246233318263	33.067921834275666	19.628451387744516
26-27	19.520699688471034	27.520896044874394	33.13131643697649	19.827087829678085
28-29	19.586033607583577	27.406604192566732	33.36642872429121	19.640933475558477
30-31	19.862934363183093	27.66665150615399	33.0341172091446	19.43629692151832
32-33	19.529286134133404	27.58195193136011	33.26846033109325	19.620301603413235
34-35	19.896850339322675	27.417635399229823	32.988139683023974	19.69737457842353
36-37	19.99492355701464	27.610322444320186	32.514076177567276	19.880677821097905
38-39	19.83475791551459	27.78544684294207	32.6095880350558	19.770207206487537
40-41	19.811003145180397	27.589162726977445	32.62983902773198	19.969995100110175
42-43	19.82630643816749	27.758411208330486	32.38162850115379	20.03365385234823
44-45	20.114250035882712	27.775859452124475	32.01312443969501	20.0967660722978
46-47	20.25760832943047	28.06329274569842	31.437892286116092	20.24120663875502
48-49	20.36924514411185	28.157580292838848	30.986489296650273	20.486685266399036
50-51	20.377110285657597	28.246628503399517	30.71346756186714	20.662793649075745
52-53	20.385200730387833	28.393131303344415	30.678234576535335	20.543433389732414
54-55	20.462105353619677	28.65184355241429	30.522034383979996	20.36401670998604
56-57	20.40978198225569	28.37007290965668	30.483611593958653	20.736533514128975
58-59	20.312215739398383	28.014985510278862	30.862783912724616	20.810014837598136
60-61	20.446933420603543	28.330242155505463	30.622979165311122	20.599845258579872
62-63	20.270486867084	28.18166146094913	30.871094648886572	20.6767570230803
64-65	20.26664608591938	28.091727943994943	30.85955070149984	20.78207526858584
66-67	20.270232836140888	28.100991305542145	30.76297890800619	20.865796950310774
68-69	20.631730032719673	28.292089620602507	30.457399033806464	20.618781312871352
70-71	20.591798557758526	28.15133266704383	30.445341563129595	20.811527212068043
72-73	20.44736803113358	28.21924409207365	30.527643548614652	20.805744328178115
74-75	20.465181180161267	28.309254444097	30.346151829259888	20.87941254648185
76-77	20.488300641923086	28.241871340689258	30.22237322656862	21.04745479081904
78-79	20.558217392363108	28.28262200295335	30.300608824574184	20.858551780109362
80-81	20.421243238458626	28.316664645972438	30.221923763608928	21.040168351960013
82-83	20.286433973689974	28.299936866246632	30.548619432311597	20.865009727751794
84-85	20.326176601213426	28.24364730882658	30.28600687228727	21.144169217672722
86-87	20.46112562262692	28.250161152770247	30.15803214654717	21.130681078055662
88-89	20.276815886757582	28.18557433182861	30.319177172165805	21.218432609248
90-91	20.224618631876766	28.215552663440675	30.166003700652965	21.39382500402959
92-93	20.244038322337353	28.2562300597759	30.162567665663143	21.337163952223605
94-95	20.0753451299909	28.06913384022962	30.362020735822924	21.493500293956554
96-97	20.165447062029443	28.15946102472558	30.170594332590984	21.504497580653993
98-99	19.987558926315756	28.137132172750544	30.191494098735948	21.68381480219775
100	20.02894843781768	28.150281405924147	30.112843247327213	21.707926908930954
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2879.0
1	1705.5
2	1702.0
3	3020.0
4	1994.0
5	1204.5
6	3016.0
7	7771.5
8	13339.0
9	14590.0
10	9637.5
11	4894.5
12	4255.0
13	4504.5
14	4885.0
15	5340.5
16	5891.0
17	6614.5
18	7354.0
19	8298.0
20	9792.0
21	12156.0
22	15704.5
23	21068.5
24	27433.5
25	34048.5
26	42804.5
27	53540.5
28	66317.5
29	78789.0
30	87470.0
31	96261.5
32	105339.5
33	113741.0
34	121510.0
35	127854.5
36	131533.0
37	133659.5
38	135599.5
39	134937.0
40	134966.5
41	134770.5
42	132719.5
43	131020.0
44	129408.0
45	126279.5
46	122641.5
47	118862.5
48	113687.0
49	109503.0
50	108249.5
51	103754.5
52	93869.5
53	85434.0
54	80067.0
55	75712.0
56	67604.0
57	58119.0
58	52242.5
59	47720.5
60	41109.0
61	33234.5
62	27255.0
63	21989.0
64	16935.0
65	13323.0
66	10411.5
67	7756.5
68	5756.5
69	4303.0
70	3184.5
71	2285.0
72	1596.0
73	1172.5
74	935.5
75	671.5
76	418.0
77	303.0
78	209.0
79	103.5
80	49.0
81	21.0
82	12.0
83	9.0
84	4.0
85	2.0
86	1.5
87	0.0
88	1.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005411387879264206
2	0.004329110303411364
3	0.0010049720347204954
4	0.0
5	0.04352301965751068
6	0.0
7	0.0
8	0.0
9	5.1537027421563865E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	7.73055411323458E-5
20-21	1.2884256855390966E-5
22-23	1.2884256855390966E-5
24-25	3.86527705661729E-5
26-27	1.6749533912008256E-4
28-29	9.018979798773676E-5
30-31	2.448008802524284E-4
32-33	0.001185351630695969
34-35	8.503609524558039E-4
36-37	9.018979798773675E-4
38-39	9.018979798773675E-4
40-41	1.4172682540930065E-4
42-43	5.153702742156386E-4
44-45	1.546110822646916E-4
46-47	1.9326385283086446E-4
48-49	1.0307405484312773E-4
50-51	2.576851371078193E-4
52-53	6.957498701911123E-4
54-55	8.117081818896309E-4
56-57	0.0020743653537179454
58-59	5.926758153479845E-4
60-61	9.663192641543224E-4
62-63	9.276664935881496E-4
64-65	0.0014301525109483972
66-67	3.73643448806338E-4
68-69	3.2210642138477414E-4
70-71	2.448008802524284E-4
72-73	1.9326385283086446E-4
74-75	6.055600722033754E-4
76-77	5.669073016372026E-4
78-79	7.85939668178849E-4
80-81	6.570970996249392E-4
82-83	0.0014172682540930064
84-85	0.0011982358875513598
86-87	0.0018166802166101263
88-89	0.0010049720347204954
90-91	9.920877778651044E-4
92-93	0.0011338146032744052
94-95	7.60171154468067E-4
96-97	1.9326385283086446E-4
98-99	2.834536508186013E-4
100	3.092221645293832E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3880705.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.37135504928089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.72327497579988	40.26786279031499
2	16.213642907426134	17.955427561386948
3	5.140426993809677	8.538972245374298
4	2.2124033934047436	4.900150952337916
5	1.0858691717401947	3.006302372274744
6	0.6314198936027715	2.0977545068314933
7	0.40000431907114475	1.5504146820773934
8	0.2782782422183473	1.2326914681889516
9	0.20801735690170695	1.0366382632875655
>10	0.9939887272117361	9.843688207265028
>50	0.07123520319151437	2.747588128325382
>100	0.038916030049149196	3.74076520143111
>500	0.0016818570796480045	0.601313957743808
>1k	7.007737442511805E-4	0.8623791135387603
>5k	0.0	0.0
>10k+	1.4015474885023538E-4	1.6180505496216149
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26663	0.6870658810705786	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21415	0.5518327211163951	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14471	0.3728961619087254	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4689	0.12082856078985647	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4521	0.11649945048644511	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3951	0.1018113976712994	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5768513710781932E-5	0.0
5	0.0	0.0	0.0	2.5768513710781932E-5	0.0
6	0.0	0.0	0.0	5.1537027421563865E-5	0.0
7	0.0	0.0	0.0	5.1537027421563865E-5	0.0
8	0.0	0.0	0.0	5.1537027421563865E-5	0.0
9	0.0	0.0	0.0	5.1537027421563865E-5	0.0
10-11	0.0	0.0	0.0	5.1537027421563865E-5	0.0
12-13	0.0	0.0	0.0	6.442128427695483E-5	0.0
14-15	0.0	0.0	0.0	7.73055411323458E-5	0.0
16-17	0.0	0.0	0.0	1.4172682540930062E-4	0.0
18-19	0.0	0.0	0.0	1.546110822646916E-4	0.0
20-21	0.0	0.0	0.0	2.1903236654164643E-4	0.0
22-23	0.0	0.0	0.0	3.73643448806338E-4	0.0
24-25	0.0	0.0	0.0	5.411387879264205E-4	0.0
26-27	0.0	1.2884256855390966E-5	0.0	0.0010307405484312774	0.0
28-29	0.0	2.5768513710781932E-5	0.0	0.0028474207650414033	0.0
30-31	0.0	2.5768513710781932E-5	0.0	0.006841540390212603	0.0
32-33	0.0	2.5768513710781932E-5	0.0	0.014700937072001093	0.0
34-35	0.0	2.5768513710781932E-5	0.0	0.0270955921668872	0.0
36-37	0.0	2.5768513710781932E-5	0.0	0.046653894073370686	0.0
38-39	0.0	2.5768513710781932E-5	0.0	0.0740071713773657	0.0
40-41	0.0	2.5768513710781932E-5	0.0	0.10172120787331168	0.0
42-43	0.0	2.5768513710781932E-5	0.0	0.129216212002716	0.0
44-45	0.0	2.5768513710781932E-5	0.0	0.16424850639252403	0.0
46-47	0.0	2.5768513710781932E-5	0.0	0.20984589140375265	0.0
48-49	0.0	2.5768513710781932E-5	0.0	0.25960489137927256	0.0
50-51	0.0	2.5768513710781932E-5	0.0	0.3108713494068732	0.0
52-53	0.0	2.5768513710781932E-5	0.0	0.3628464415615204	0.0
54-55	0.0	2.5768513710781932E-5	0.0	0.42238459249028204	0.0
56-57	0.0	2.5768513710781932E-5	0.0	0.493595880130028	0.0
58-59	0.0	2.5768513710781932E-5	0.0	0.5753851426480497	0.0
60-61	0.0	2.5768513710781932E-5	0.0	0.6627146356138898	0.0
62-63	0.0	2.5768513710781932E-5	0.0	0.7511006376418717	0.0
64-65	0.0	2.5768513710781932E-5	0.0	0.842720588140557	0.0
66-67	0.0	2.5768513710781932E-5	0.0	0.9550841921764215	0.0
68-69	0.0	2.5768513710781932E-5	0.0	1.0827542933564906	0.0
70-71	0.0	2.5768513710781932E-5	0.0	1.2269677803388817	0.0
72-73	0.0	2.5768513710781932E-5	0.0	1.3784608724445686	0.0
74-75	0.0	2.5768513710781932E-5	0.0	1.5339609684322824	0.0
76-77	0.0	2.5768513710781932E-5	0.0	1.7021134046519897	0.0
78-79	0.0	2.5768513710781932E-5	0.0	1.902978969027535	0.0
80-81	0.0	2.5768513710781932E-5	0.0	2.1248072193068013	0.0
82-83	0.0	2.5768513710781932E-5	0.0	2.3679589146817395	0.0
84-85	0.0	2.5768513710781932E-5	0.0	2.6070263006335193	0.0
86-87	0.0	2.5768513710781932E-5	0.0	2.86395126658687	0.0
88	0.0	2.5768513710781932E-5	0.0	3.0713491491880984	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10090	0.0	62.31167	1
GTATCAA	20055	0.0	45.77056	1
GTGGTAT	3350	0.0	35.795177	1
ATCAACG	25900	0.0	35.038204	3
TCAACGC	25985	0.0	34.887405	4
TATCAAC	26420	0.0	34.510536	2
CAACGCA	26930	0.0	33.68063	5
AACGCAG	27870	0.0	32.597008	6
TGGTATC	3625	0.0	31.132212	2
ACGCAGA	31680	0.0	28.52837	7
CGCAGAG	31710	0.0	28.456917	8
TATAACG	315	0.0	28.362843	2
GTACATG	19315	0.0	26.781017	1
GCAGAGT	34135	0.0	26.215004	9
CATGGGT	8130	0.0	26.025934	4
TACATGG	19475	0.0	25.714546	2
ACATGGG	19530	0.0	25.038912	3
TACCTGG	3020	0.0	24.756937	2
GAGTACT	23620	0.0	22.892307	12-13
GTACCTG	3355	0.0	22.706573	1
>>END_MODULE