FastQCFastQC Report
Wed 25 May 2016
SRR2431387_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431387_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4972026
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA164400.33064992017338607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.044.774271
GTATCAA94700.037.615691
ATCAACG103850.032.395723
TCAACGC104450.032.2067074
CAACGCA112850.029.7677065
TATCAAC115800.029.6621342
AACGCAG120650.028.0833746
GTACATG191200.025.202011
ACGCAGA133450.025.1082657
CGCAGAG133400.024.8358488
TACATGG191000.024.3395022
ACATGGG195550.022.7625753
CATGGGT72800.021.8440934
GCAGAGT153300.020.8148529
GTCTCGC11400.017.7495251
AGAGTAC136900.017.74723210-11
CAGAGTA151350.017.3879810-11
ATGGGTG45000.017.3557875
CATGGGG101750.016.3225884
CTGTCGC12100.016.3120929
Produced by FastQC (version 0.11.5)