FastQCFastQC Report
Wed 25 May 2016
SRR2431387_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431387_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4972026
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272160.5473824955863063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222310.4471215556797169No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145570.2927780345476874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118050.062.18531
GTATCAA231450.044.3961261
ATCAACG290850.035.0799063
TCAACGC293000.034.80614
TATCAAC296950.034.441922
CAACGCA302550.033.738525
AACGCAG314600.032.4770366
TGGTATC40400.031.0771622
GTGGTAT42000.030.0079481
ACGCAGA350850.029.121497
CGCAGAG352950.028.9482238
GCAGAGT381300.026.5493839
TATAACG3800.024.7490122
GTACATG189750.024.0155641
GAGTACT271800.023.19619612-13
TACATGG190250.023.1840672
CAGAGTA377450.022.59918610-11
ACATGGG192650.022.282793
AGAGTAC361350.021.94781510-11
CATGGGT71650.021.0645524
Produced by FastQC (version 0.11.5)