##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431387_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4972026 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.651973058869764 34.0 31.0 34.0 31.0 34.0 2 32.76941009560288 34.0 31.0 34.0 31.0 34.0 3 32.848760646062594 34.0 31.0 34.0 31.0 34.0 4 36.3124227427612 37.0 37.0 37.0 35.0 37.0 5 36.215615726868684 37.0 37.0 37.0 35.0 37.0 6 36.2628419481314 37.0 37.0 37.0 35.0 37.0 7 36.21878063389049 37.0 37.0 37.0 35.0 37.0 8 36.23326748492465 37.0 37.0 37.0 35.0 37.0 9 38.03029388824596 39.0 39.0 39.0 35.0 39.0 10-11 38.01090028491404 39.0 39.0 39.0 35.0 39.0 12-13 37.991146164561485 39.0 39.0 39.0 35.0 39.0 14-15 39.47166426724237 41.0 39.0 41.0 36.0 41.0 16-17 39.50752751494059 41.0 39.0 41.0 36.0 41.0 18-19 39.569337630173294 41.0 40.0 41.0 36.5 41.0 20-21 39.56308585272885 41.0 40.0 41.0 37.0 41.0 22-23 39.51204639718296 41.0 39.5 41.0 36.0 41.0 24-25 39.469450280428944 41.0 39.0 41.0 36.0 41.0 26-27 39.35861658808703 41.0 39.0 41.0 36.0 41.0 28-29 39.28162815319148 41.0 39.0 41.0 36.0 41.0 30-31 39.1230459172981 41.0 39.0 41.0 35.5 41.0 32-33 38.92431063313024 41.0 38.5 41.0 35.0 41.0 34-35 38.80255634222347 41.0 38.0 41.0 35.0 41.0 36-37 38.66937894130079 41.0 38.0 41.0 35.0 41.0 38-39 38.52478768212394 41.0 38.0 41.0 35.0 41.0 40-41 38.393938205471976 41.0 38.0 41.0 35.0 41.0 42-43 38.23933332207032 40.5 38.0 41.0 34.0 41.0 44-45 38.029674925271905 40.0 38.0 41.0 34.0 41.0 46-47 37.719336745222165 40.0 38.0 41.0 33.0 41.0 48-49 37.428197579819575 40.0 38.0 41.0 33.0 41.0 50-51 37.27275048441018 40.0 37.5 41.0 32.5 41.0 52-53 37.21811490929453 40.0 37.0 41.0 32.5 41.0 54-55 37.082086055060856 40.0 37.0 41.0 32.0 41.0 56-57 36.9392552452461 40.0 37.0 41.0 32.0 41.0 58-59 36.76796581514256 40.0 36.0 41.0 31.0 41.0 60-61 36.61779101718294 40.0 36.0 41.0 31.0 41.0 62-63 36.46247726781799 39.5 35.5 41.0 31.0 41.0 64-65 36.229731401243676 39.0 35.0 41.0 31.0 41.0 66-67 35.96783252943568 39.0 35.0 41.0 31.0 41.0 68-69 35.67520855683377 38.5 35.0 41.0 30.0 41.0 70-71 35.37048740292187 37.0 35.0 40.0 30.0 41.0 72-73 35.046639639454824 37.0 35.0 40.0 30.0 41.0 74-75 34.70349913697153 36.5 35.0 39.0 29.0 41.0 76-77 34.364858610956574 36.0 35.0 39.0 29.0 41.0 78-79 34.03873662366206 36.0 35.0 38.5 29.0 40.0 80-81 33.76433771665715 35.0 35.0 37.0 29.0 39.0 82-83 33.55741924921551 35.0 35.0 37.0 29.0 39.0 84-85 33.287975264007066 35.0 34.5 36.5 29.0 39.0 86-87 33.038180512330385 35.0 34.0 36.0 29.0 37.0 88-89 32.7998196308708 35.0 34.0 36.0 29.0 37.0 90-91 32.61537027360677 35.0 34.0 35.5 29.0 37.0 92-93 32.451678350032765 35.0 34.0 35.0 29.0 36.0 94-95 32.31073339117695 35.0 34.0 35.0 29.0 36.0 96-97 32.168286630037734 35.0 34.0 35.0 29.0 36.0 98-99 32.052552018030475 35.0 34.0 35.0 29.0 36.0 100 31.950748246288335 35.0 34.0 35.0 27.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.005762642552298303 1101 2 8.285724930203742E-4 1101 3 -0.010451782112703256 1101 4 0.03143992433724918 1101 5 0.020138414224270207 1101 6 0.0026606859742059896 1101 7 -0.015272864818932419 1101 8 0.03605459408339584 1101 9 -0.023666051986140246 1101 10-11 0.030553342359503688 1101 12-13 0.011402860456243502 1101 14-15 0.00686817043253285 1101 16-17 -0.02427066938160749 1101 18-19 0.03114925976955618 1101 20-21 0.05375048548962269 1101 22-23 0.025835798771154828 1101 24-25 0.03540170755916705 1101 26-27 0.05148436012014912 1101 28-29 0.03657255482496424 1101 30-31 0.010815692929632803 1101 32-33 0.05638235701002259 1101 34-35 0.08995888452441392 1101 36-37 0.08789991419035914 1101 38-39 0.03507858617057025 1101 40-41 0.006945056665422555 1101 42-43 0.04386081468926051 1101 44-45 0.05188605132984492 1101 46-47 0.06622109121779118 1101 48-49 0.0665295908486101 1101 50-51 -0.020419472822261753 1101 52-53 0.01023003746478679 1101 54-55 0.03058875816874007 1101 56-57 0.07247891132765716 1101 58-59 0.07087973639443845 1101 60-61 0.04875651106677026 1101 62-63 0.07691820051002907 1101 64-65 0.05419538430541593 1101 66-67 0.04825604599179201 1101 68-69 0.04450441604230804 1101 70-71 0.07601875605978137 1101 72-73 0.05689287785182273 1101 74-75 -0.011603607592512333 1101 76-77 -0.009980596672647835 1101 78-79 0.052988893532322834 1101 80-81 0.10171074460794216 1101 82-83 0.07868561596212231 1101 84-85 0.06731988360091634 1101 86-87 0.12973401856328337 1101 88-89 0.1613533613727398 1101 90-91 0.12034759448106769 1101 92-93 0.08212159493633919 1101 94-95 0.046974670529017715 1101 96-97 0.1397049731819564 1101 98-99 0.17062179154312673 1101 100 0.17521637730632023 1102 1 -0.06610786664930401 1102 2 -0.030945503518665873 1102 3 -0.014476484066598516 1102 4 0.016368226499153593 1102 5 0.017394813040034762 1102 6 -0.03491860890292742 1102 7 -0.019442397062853445 1102 8 -0.030577508350219773 1102 9 0.0026699067270214982 1102 10-11 0.03448310088130313 1102 12-13 -0.003966476730255408 1102 14-15 -0.048611686775679175 1102 16-17 -0.05098698849729999 1102 18-19 -0.01716151763782392 1102 20-21 0.030194345311109316 1102 22-23 0.011026324719047409 1102 24-25 0.007868464255544438 1102 26-27 0.01162693132362591 1102 28-29 -0.02703899711744384 1102 30-31 -0.024313161832381525 1102 32-33 -0.025999188787167782 1102 34-35 -0.06616824635430163 1102 36-37 -0.0882292849102555 1102 38-39 -0.028109758601090107 1102 40-41 -0.10784219808132178 1102 42-43 -0.12777309157178962 1102 44-45 -0.083751778451429 1102 46-47 -0.19736086496969563 1102 48-49 -0.2151430100921914 1102 50-51 -0.23810573482248998 1102 52-53 -0.23621489323257094 1102 54-55 -0.17883891148022002 1102 56-57 -0.16432987701971769 1102 58-59 -0.13152249083963596 1102 60-61 -0.17197521551992168 1102 62-63 -0.16041675632014574 1102 64-65 -0.22438671503056185 1102 66-67 -0.16643800187831914 1102 68-69 -0.1554362047121245 1102 70-71 -0.18513798700072215 1102 72-73 -0.1358199370201092 1102 74-75 -0.20206226396167182 1102 76-77 -0.21392090750669013 1102 78-79 -0.19059981410120486 1102 80-81 -0.21955189861094482 1102 82-83 -0.2550429141109518 1102 84-85 -0.24094505265341581 1102 86-87 -0.2439889287186503 1102 88-89 -0.23935771115520765 1102 90-91 -0.21321132809734422 1102 92-93 -0.20641198410134365 1102 94-95 -0.23166388641529068 1102 96-97 -0.2155064297608682 1102 98-99 -0.25330524793957565 1102 100 -0.28124211495051554 1103 1 -0.08862370083760851 1103 2 -0.11960614517293067 1103 3 -0.09159176661069779 1103 4 -0.049411881808147484 1103 5 -0.07525855766715495 1103 6 -0.057269076763091675 1103 7 -0.09315601470769508 1103 8 -0.04394812622087585 1103 9 -0.038852399039825514 1103 10-11 -0.029117548956264727 1103 12-13 -0.023103780067344815 1103 14-15 -0.0634407486874764 1103 16-17 -0.06389868640400209 1103 18-19 -0.11592208114171143 1103 20-21 -0.13414366362031416 1103 22-23 -0.1165275292292236 1103 24-25 -0.12870825608315783 1103 26-27 -0.09304119831856639 1103 28-29 -0.13390194263298127 1103 30-31 -0.0837007900647393 1103 32-33 -0.1021861123654304 1103 34-35 -0.0852822785236711 1103 36-37 -0.11196667910675728 1103 38-39 -0.16358603478173706 1103 40-41 -0.11816409709636133 1103 42-43 -0.07893212109044612 1103 44-45 -0.12304220685560097 1103 46-47 -0.02429319069019442 1103 48-49 0.03396112830496634 1103 50-51 0.011859064598063185 1103 52-53 0.0590576748246221 1103 54-55 0.05062992450851311 1103 56-57 0.06042813417770532 1103 58-59 0.054421817886805 1103 60-61 0.06332279333001622 1103 62-63 0.002167903326103726 1103 64-65 0.027185107393343344 1103 66-67 0.08591025091143933 1103 68-69 0.09513243058040644 1103 70-71 0.08867748705647216 1103 72-73 0.08744781837155813 1103 74-75 0.1096650960520762 1103 76-77 0.08722451582248425 1103 78-79 0.13280397184354342 1103 80-81 0.14120150426844447 1103 82-83 0.055046218379096956 1103 84-85 0.07856361385853461 1103 86-87 0.1279743993686111 1103 88-89 0.06666666527544152 1103 90-91 0.08873418853589499 1103 92-93 0.08369889008438491 1103 94-95 0.07787516111540782 1103 96-97 0.1483587082590887 1103 98-99 0.14169585058490952 1103 100 0.1642628827090462 1104 1 -0.12544534730788826 1104 2 -0.09993368155177507 1104 3 -0.11881801684308613 1104 4 -0.06431242094181755 1104 5 -0.03934025585370904 1104 6 -0.062100153847374884 1104 7 -0.1015455430079939 1104 8 -0.1525675046115893 1104 9 -0.10046067364307731 1104 10-11 -0.14657094516035585 1104 12-13 -0.10169856787852893 1104 14-15 -0.15757830382614912 1104 16-17 -0.1530112284445977 1104 18-19 -0.10605838562548087 1104 20-21 -0.08406127250334805 1104 22-23 -0.09296282473869155 1104 24-25 -0.10833398915284675 1104 26-27 -0.13480081906590868 1104 28-29 -0.07801794932567674 1104 30-31 -0.07338668977573803 1104 32-33 -0.11688173000304403 1104 34-35 -0.13119525660340514 1104 36-37 -0.13081354148401658 1104 38-39 -0.13817069904850143 1104 40-41 -0.22499368691536148 1104 42-43 -0.2131755593773903 1104 44-45 -0.15779604754921195 1104 46-47 -0.21533537494208588 1104 48-49 -0.21969450217721231 1104 50-51 -0.341633051205811 1104 52-53 -0.29804105930980285 1104 54-55 -0.21566788346525811 1104 56-57 -0.26801074428905025 1104 58-59 -0.20750926088755506 1104 60-61 -0.20234165933946002 1104 62-63 -0.21577814950561702 1104 64-65 -0.15452195204656505 1104 66-67 -0.16845943591781065 1104 68-69 -0.22601800292362384 1104 70-71 -0.1436772933183903 1104 72-73 -0.1522283634128243 1104 74-75 -0.12912168418757375 1104 76-77 -0.09480365771433696 1104 78-79 -0.12317001791460314 1104 80-81 -0.08703245270226745 1104 82-83 -0.12348181150142068 1104 84-85 -0.1499969499502214 1104 86-87 -0.10014294248269096 1104 88-89 -0.07862521053790772 1104 90-91 -0.08461587653195579 1104 92-93 -0.10933546912398384 1104 94-95 -0.10468961097809171 1104 96-97 -0.09405201357159143 1104 98-99 -0.1143791211063494 1104 100 -0.0478042470379485 1105 1 -0.11899982106161389 1105 2 -0.10291681299069921 1105 3 -0.1306278964611849 1105 4 -0.04841370778283505 1105 5 -0.11002480998782715 1105 6 -0.02114426871963815 1105 7 -0.0035486374643838303 1105 8 -0.044648337850581754 1105 9 -0.048434182077357946 1105 10-11 -0.057148514125223926 1105 12-13 -0.05834749802683348 1105 14-15 -0.12108107751572561 1105 16-17 -0.1517979751142846 1105 18-19 -0.1418153953239596 1105 20-21 -0.08094228688124616 1105 22-23 -0.11807452706708688 1105 24-25 -0.17980819572667173 1105 26-27 -0.05330642305097655 1105 28-29 -0.07387722703426647 1105 30-31 -0.028189135151038158 1105 32-33 -0.05862500333411447 1105 34-35 -0.050266730998664855 1105 36-37 -0.07587392112098712 1105 38-39 0.004181653870482194 1105 40-41 -0.042178108969018524 1105 42-43 -0.10075301513043655 1105 44-45 -0.07850305536082658 1105 46-47 -0.06291660442244051 1105 48-49 -0.0503494325519398 1105 50-51 -0.0709011367939496 1105 52-53 -0.05162213784107195 1105 54-55 -0.024266132455785794 1105 56-57 0.014164620819066442 1105 58-59 -0.04320475883087482 1105 60-61 -0.05535818826507466 1105 62-63 -0.09579116886484229 1105 64-65 -0.05679486800565314 1105 66-67 -0.12859887514193247 1105 68-69 -0.030450811708860215 1105 70-71 -0.019477323508205302 1105 72-73 -0.022082508852271587 1105 74-75 -0.0485412849272322 1105 76-77 -0.05815397282056267 1105 78-79 0.07654695253711452 1105 80-81 0.043235670623140265 1105 82-83 -0.029546757348434483 1105 84-85 -9.02078445633947E-4 1105 86-87 -0.008782891159995643 1105 88-89 0.018097495979318978 1105 90-91 1.0599976526037835E-4 1105 92-93 -0.041785184207576265 1105 94-95 -0.06443771468011761 1105 96-97 -0.06405635335799786 1105 98-99 -0.06411571494007973 1105 100 -0.06313086313667071 1106 1 -0.12889552049971797 1106 2 -0.10367152304248606 1106 3 -0.10180201229356811 1106 4 -0.051001022777874994 1106 5 -0.09778344967345731 1106 6 -0.09938034055480927 1106 7 -0.07756604537507172 1106 8 -0.05081823024471532 1106 9 -0.09121577523171709 1106 10-11 -0.05294913111445965 1106 12-13 -0.09321142986421194 1106 14-15 -0.11090956919738915 1106 16-17 -0.16664611496518233 1106 18-19 -0.134293084552354 1106 20-21 -0.13435041791800728 1106 22-23 -0.16073455865105757 1106 24-25 -0.09228955751006396 1106 26-27 -0.15972770183461904 1106 28-29 -0.14897822229666957 1106 30-31 -0.20800991446719763 1106 32-33 -0.1889500244513087 1106 34-35 -0.23161198789388493 1106 36-37 -0.18258349547186015 1106 38-39 -0.19844403932427213 1106 40-41 -0.28467618087764635 1106 42-43 -0.2994459528071758 1106 44-45 -0.26621505359800324 1106 46-47 -0.28795131006716446 1106 48-49 -0.3066790810884257 1106 50-51 -0.380009073185299 1106 52-53 -0.3698519663327531 1106 54-55 -0.33649041538942015 1106 56-57 -0.3583725638002804 1106 58-59 -0.3301480913072723 1106 60-61 -0.3369399577020715 1106 62-63 -0.3803190619200407 1106 64-65 -0.3783103478249643 1106 66-67 -0.3699459683146529 1106 68-69 -0.39804179925889827 1106 70-71 -0.3426988887511442 1106 72-73 -0.2995040079892206 1106 74-75 -0.287743055098737 1106 76-77 -0.32902436835473736 1106 78-79 -0.33816071208516973 1106 80-81 -0.3208412326784824 1106 82-83 -0.3313062784472649 1106 84-85 -0.3288893346798005 1106 86-87 -0.3309550238126917 1106 88-89 -0.36651009151930936 1106 90-91 -0.3686844442466395 1106 92-93 -0.29645538876888367 1106 94-95 -0.31478422024403585 1106 96-97 -0.34226359563645303 1106 98-99 -0.30846567270569736 1106 100 -0.2963862158855761 1107 1 -0.2456820671380271 1107 2 -0.22757691527184676 1107 3 -0.17317032406094768 1107 4 -0.20848553937846503 1107 5 -0.27292162635868067 1107 6 -0.21739134299453156 1107 7 -0.18216116507075952 1107 8 -0.14474592316435775 1107 9 -0.18849955690035358 1107 10-11 -0.14121804182698128 1107 12-13 -0.12751450098374306 1107 14-15 -0.2257177373098429 1107 16-17 -0.20811900878489098 1107 18-19 -0.2074692169262633 1107 20-21 -0.18134345056776624 1107 22-23 -0.1564256287539152 1107 24-25 -0.15046854747404126 1107 26-27 -0.21035956669215494 1107 28-29 -0.26803109632864874 1107 30-31 -0.2591393446292827 1107 32-33 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68-69 0.2002408231782269 2214 70-71 0.19771476201281502 2214 72-73 0.20141245399047847 2214 74-75 0.21762885584375624 2214 76-77 0.17625037250935804 2214 78-79 0.20269991790084418 2214 80-81 0.17640778071253038 2214 82-83 0.17333274538263055 2214 84-85 0.16284624971849837 2214 86-87 0.1578006781814949 2214 88-89 0.13555215194195824 2214 90-91 0.1710594674014061 2214 92-93 0.1344104229935894 2214 94-95 0.11118019906117382 2214 96-97 0.14504417489401789 2214 98-99 0.13833427431526957 2214 100 0.1729763973618148 2215 1 0.10784899199569509 2215 2 0.09802916433094566 2215 3 0.10346713425563081 2215 4 0.045768923885290747 2215 5 0.05107446899861401 2215 6 0.061100599784808196 2215 7 0.03918839025291021 2215 8 0.004968564865926339 2215 9 0.02285548464996623 2215 10-11 0.05509440908588914 2215 12-13 0.041994904975183545 2215 14-15 0.10964740346118873 2215 16-17 0.06229206686639799 2215 18-19 0.08453219094352704 2215 20-21 0.06731118483983778 2215 22-23 0.016140735327887512 2215 24-25 0.05064094740298941 2215 26-27 0.05472672851247751 2215 28-29 0.08226189908976522 2215 30-31 0.07311709794530685 2215 32-33 0.05926200022388883 2215 34-35 0.009567354305580977 2215 36-37 -0.05820937126813419 2215 38-39 -0.04312533851777545 2215 40-41 -0.013956483582312273 2215 42-43 -0.01791762459711066 2215 44-45 -0.009723507296392597 2215 46-47 0.08577302088895777 2215 48-49 0.08055280704544288 2215 50-51 0.0724898859910823 2215 52-53 1.242021836560525E-4 2215 54-55 0.030320022075805753 2215 56-57 0.059335806632226706 2215 58-59 0.08109145286724839 2215 60-61 0.09181084517514648 2215 62-63 0.10269442027665576 2215 64-65 0.07338733067781789 2215 66-67 0.042447692670364745 2215 68-69 0.03907249729785178 2215 70-71 0.07857189241308049 2215 72-73 0.09164272787737815 2215 74-75 0.06703251385413722 2215 76-77 0.09022512719889164 2215 78-79 0.13968693835529677 2215 80-81 0.0696588723249576 2215 82-83 0.054394155935412414 2215 84-85 0.051737621853689575 2215 86-87 0.047333487582335465 2215 88-89 0.06269268671218953 2215 90-91 0.04978458710579048 2215 92-93 0.027919288084547134 2215 94-95 -0.015068742736268348 2215 96-97 -0.004286305855195849 2215 98-99 -0.004603418803682757 2215 100 0.04191836533489379 2216 1 0.1292976261784986 2216 2 0.1015190502918415 2216 3 0.08620455499482915 2216 4 0.07413404190250361 2216 5 0.06884636667684418 2216 6 0.06874941720169403 2216 7 0.05139997739758684 2216 8 0.04600335523167587 2216 9 -0.015795454424704758 2216 10-11 0.02850889181126348 2216 12-13 0.03334402617488763 2216 14-15 0.03552499827276989 2216 16-17 0.02914568453402211 2216 18-19 -0.002754136418623432 2216 20-21 -0.009259866586255328 2216 22-23 -0.0712209349954307 2216 24-25 -0.020909249091452864 2216 26-27 0.02084349201997071 2216 28-29 -0.0021086556755207653 2216 30-31 -0.04382271206988264 2216 32-33 0.01084510652376025 2216 34-35 0.06455415421878996 2216 36-37 0.06147894997014447 2216 38-39 0.07241158556062999 2216 40-41 0.056924667417035835 2216 42-43 0.08557346685115874 2216 44-45 0.05668528552854468 2216 46-47 0.052138416168268975 2216 48-49 0.0030309274495223804 2216 50-51 0.07076603903976064 2216 52-53 0.024928611856488203 2216 54-55 0.05359949021477206 2216 56-57 0.03493674209043007 2216 58-59 0.033325522351539405 2216 60-61 -0.021468803786930835 2216 62-63 -0.04168228247426242 2216 64-65 -0.03283268282363849 2216 66-67 0.010753922629135104 2216 68-69 0.04956174435043437 2216 70-71 0.019070542267215274 2216 72-73 -0.001758434212455029 2216 74-75 -0.021292039512786687 2216 76-77 -0.0037049424782793494 2216 78-79 0.02697236160191352 2216 80-81 0.06329389896622928 2216 82-83 0.08484175339906841 2216 84-85 0.09859943683548522 2216 86-87 0.036793007316163084 2216 88-89 0.11643331404782487 2216 90-91 0.08537810881661301 2216 92-93 0.03680071634599358 2216 94-95 2.2437151982757086E-4 2216 96-97 -0.00368657588436605 2216 98-99 0.023868686218378343 2216 100 0.04594469317924066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41.0 3 224.0 4 58.0 5 118.0 6 288.0 7 744.0 8 1462.0 9 2695.0 10 4148.0 11 5844.0 12 8944.0 13 13245.0 14 15192.0 15 20006.0 16 24608.0 17 35951.0 18 41636.0 19 21814.0 20 13617.0 21 11953.0 22 11805.0 23 12141.0 24 13226.0 25 15961.0 26 19909.0 27 25269.0 28 31783.0 29 41087.0 30 52180.0 31 65858.0 32 83742.0 33 109236.0 34 150534.0 35 238682.0 36 391706.0 37 835624.0 38 1596334.0 39 1044109.0 40 10252.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.78983335099997 21.25692443259428 14.880462585586802 23.07277963081895 2 14.179374208659464 21.961850723633464 40.7579772657104 23.100797801996666 3 16.249743562926643 28.1229610738579 31.911673015579307 23.715622347636153 4 11.070034629746505 18.755815033951954 40.16823323128238 30.005917105019158 5 11.272518597989654 38.907304223109875 36.04985782387223 13.770319355028239 6 27.473106536450132 40.41410885622883 18.548656020704637 13.5641285866164 7 24.02638280652595 34.67254596013778 24.10180477736842 17.199266455967848 8 24.17974081390564 35.80622868826511 21.512860149967036 18.50117034786222 9 25.003569973286545 16.299613075233317 22.59410952396468 36.10270742751546 10-11 22.432223805748404 27.663381084491512 30.01076422367864 19.893630886081446 12-13 24.084168514421336 25.791148898974875 29.981030869612397 20.143651716991396 14-15 21.365284493685273 27.0433722591153 28.51303472668888 23.07830852051055 16-17 19.37851083024413 30.302921817275475 28.978059344225006 21.340508008255387 18-19 19.254422343898582 28.127358194570263 32.73918227265194 19.879037188879217 20-21 20.753280453481135 27.321337418589525 32.50490846186243 19.42047366606691 22-23 20.288496164892187 27.406461894948425 32.516090779520496 19.78895116063889 24-25 20.0501304800358 27.48573267431278 32.466731361970226 19.997405483681195 26-27 19.843649107309773 27.34308369342329 32.62528128649484 20.1879859127721 28-29 19.850455131963983 27.37145339031756 32.69102222949849 20.08706924821996 30-31 20.158322176719754 27.79438350278011 32.256800992193604 19.79049332830653 32-33 19.81471289136607 27.54607112145624 32.60221649029925 20.036999496878444 34-35 20.02086693832236 27.38261215853644 32.416894275520015 20.17962662762119 36-37 20.355537570263984 27.656716742116394 31.86635100334596 20.121394684273657 38-39 20.301868364612528 27.77956055031322 31.854003097565887 20.06456798750837 40-41 20.143304015251758 27.652037392470703 31.890397800329325 20.314260791948218 42-43 20.126323322177868 27.666734345621435 31.742827614662783 20.46411471753791 44-45 20.49644218978246 27.765943774551143 31.400372766073502 20.337241269592894 46-47 20.52386152911694 28.13323357508618 30.790543883572475 20.552361012224406 48-49 20.587773001383844 28.137086696024948 30.49131022462393 20.78383007796728 50-51 20.66908278946435 28.11814444263096 30.268338865797983 20.944433902106702 52-53 20.637394813222638 28.228461486942148 30.18776791340447 20.946375786430742 54-55 20.759503803613168 28.62729015766948 29.97221631691017 20.640989721807173 56-57 20.615558206001317 28.241953293603466 30.05202226106265 21.09046623933257 58-59 20.43179644872969 27.903514796021035 30.4644483286439 21.200240426605372 60-61 20.73975193706149 28.276652847848453 30.066156955279645 20.91743825981041 62-63 20.599584792679927 28.152781973517964 30.242233768720432 21.005399465081677 64-65 20.584165107838462 27.95765967501084 30.368735569672307 21.08943964747839 66-67 20.56292151610793 28.08028748951002 30.13383427116713 21.22295672321492 68-69 21.01036712584004 28.165937569168392 29.965680912636117 20.858014392355454 70-71 20.954254489183143 28.006999990144827 30.03171556274961 21.007029957922423 72-73 20.78906979237207 28.162854968977392 30.10526508446569 20.942810154184848 74-75 20.867212296728734 28.222045543544045 29.87512643330466 21.035615726422566 76-77 20.931889981732677 28.271613442699277 29.674572115706127 21.121924459861916 78-79 20.998244160274176 28.329247100752617 29.728659407318613 20.943849331654594 80-81 20.78862868653063 28.15044695428486 29.920283347596584 21.140641011587928 82-83 20.72781595879266 28.208361742824216 29.998773112252188 21.06504918613094 84-85 20.719669055533924 28.216251886582977 29.841197179699243 21.22288187818386 86-87 20.846566827241887 28.27007168462386 29.666364100607474 21.216997387526785 88-89 20.619451200938542 28.184819464254122 29.80729653091921 21.388432803888122 90-91 20.55902294477199 28.079627744427977 29.808543330141095 21.55280598065894 92-93 20.61967306457885 28.273424537463644 29.66589336339257 21.441009034564935 94-95 20.455315451193854 28.118356836203027 29.842377732097354 21.58394998050576 96-97 20.46816188070059 28.121872812445726 29.774561423180256 21.635403883673423 98-99 20.449551126668442 28.12280521093708 29.69738157893678 21.730262083457696 100 20.46638580166898 28.18280534213351 29.546194595718724 21.80461426047879 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2502.0 1 1433.5 2 1951.5 3 3534.0 4 2189.5 5 1188.5 6 2862.5 7 7566.5 8 13351.0 9 14689.0 10 9616.0 11 4808.5 12 4174.5 13 4525.5 14 5019.0 15 5695.0 16 6247.5 17 7091.5 18 8258.0 19 9596.5 20 11498.0 21 14173.5 22 18287.0 23 24384.0 24 31832.0 25 40718.5 26 52640.5 27 66537.5 28 82526.5 29 99770.0 30 114155.0 31 126568.0 32 137319.5 33 147384.5 34 156938.0 35 163722.0 36 167549.0 37 171543.0 38 173940.0 39 173650.5 40 173450.0 41 171059.0 42 167113.5 43 162562.5 44 158584.5 45 154641.0 46 150917.5 47 146716.0 48 142250.0 49 139778.0 50 137956.5 51 132716.5 52 122826.0 53 113281.5 54 105520.0 55 99651.0 56 91384.0 57 81010.5 58 73497.5 59 66909.0 60 58264.5 61 48664.5 62 40546.0 63 32963.5 64 25928.5 65 20388.5 66 16145.5 67 12250.0 68 9206.5 69 6990.5 70 5048.5 71 3604.0 72 2693.5 73 2040.0 74 1602.0 75 1225.5 76 822.5 77 593.5 78 434.0 79 261.5 80 143.5 81 77.0 82 45.5 83 31.5 84 10.5 85 6.0 86 8.0 87 6.0 88 4.5 89 3.5 90 2.0 91 1.0 92 0.5 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006415895652999401 2 0.004847118659476036 3 9.251761756676252E-4 4 0.0 5 0.04292012954075461 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.0056262778995927E-5 14-15 0.0 16-17 1.0056262778995927E-5 18-19 4.022505111598371E-5 20-21 0.0 22-23 3.0168788336987778E-5 24-25 2.0112525557991854E-5 26-27 9.050636501096334E-5 28-29 1.3073141612694703E-4 30-31 2.514065694748982E-4 32-33 0.0013374829496064582 34-35 9.251761756676252E-4 36-37 0.0012469765845954947 38-39 0.0011564702195845315 40-41 1.8101273002192668E-4 42-43 4.525318250548167E-4 44-45 1.4078767890594296E-4 46-47 1.2067515334795111E-4 48-49 9.050636501096334E-5 50-51 2.1118151835891445E-4 52-53 7.441634456456986E-4 54-55 8.648385989936496E-4 56-57 0.0023330529647270547 58-59 6.134320295187515E-4 60-61 0.0012469765845954947 62-63 0.0010458513290155763 64-65 0.0015184956796283847 66-67 3.720817228228493E-4 68-69 2.312940439169063E-4 70-71 3.821379856018452E-4 72-73 1.4078767890594296E-4 74-75 7.441634456456986E-4 76-77 6.73769606192727E-4 78-79 9.251761756676252E-4 80-81 8.246135478776661E-4 82-83 0.0019308024535672178 84-85 0.0014883268912913971 86-87 0.002192265285821112 88-89 0.001216807796258507 90-91 0.0013173704240484663 92-93 0.0013173704240484663 94-95 8.950073873306375E-4 96-97 1.3073141612694703E-4 98-99 4.4247556227582075E-4 100 1.0056262778995926E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4972026.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.77382021707023 #Duplication Level Percentage of deduplicated Percentage of total 1 75.21092476247325 46.460661446366174 2 15.575639219281918 19.243334737957383 3 4.617200628171853 8.556663645324953 4 1.7879760107429625 4.4180043456028075 5 0.8664294370136956 2.67613281364307 6 0.4951897591916394 1.8353857894583177 7 0.3228978078678169 1.3962641792198824 8 0.20776768105322752 1.026768270103973 9 0.15015747428197893 0.8348220738489882 >10 0.7054367854141932 7.63985924097828 >50 0.04074004818525641 1.7370598632837642 >100 0.01840280629719473 1.9108373138889942 >500 5.536552057248929E-4 0.22319417427740187 >1k 5.862212735536007E-4 0.7533537636626791 >5k 0.0 0.0 >10k+ 9.770354558994818E-5 1.2876583423834715 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27216 0.5473824955863063 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22231 0.4471215556797169 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14557 0.2927780345476874 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.0112525557991854E-5 0.0 4 0.0 0.0 0.0 6.0337576673975555E-5 0.0 5 0.0 0.0 0.0 6.0337576673975555E-5 0.0 6 0.0 0.0 0.0 6.0337576673975555E-5 0.0 7 0.0 0.0 0.0 6.0337576673975555E-5 0.0 8 0.0 0.0 0.0 6.0337576673975555E-5 0.0 9 0.0 0.0 0.0 6.0337576673975555E-5 0.0 10-11 0.0 0.0 0.0 6.0337576673975555E-5 0.0 12-13 0.0 0.0 0.0 6.0337576673975555E-5 0.0 14-15 0.0 0.0 0.0 1.0056262778995926E-4 0.0 16-17 0.0 0.0 0.0 1.1061889056895519E-4 0.0 18-19 0.0 0.0 0.0 1.4078767890594296E-4 0.0 20-21 0.0 0.0 0.0 2.0112525557991853E-4 0.0 22-23 0.0 0.0 0.0 3.016878833698778E-4 0.0 24-25 0.0 1.0056262778995927E-5 0.0 4.223630367178289E-4 0.0 26-27 0.0 2.0112525557991854E-5 0.0 9.352324384466211E-4 0.0 28-29 0.0 2.0112525557991854E-5 0.0 0.002041421344136173 2.0112525557991854E-5 30-31 0.0 2.0112525557991854E-5 0.0 0.004806893608360053 2.0112525557991854E-5 32-33 0.0 2.0112525557991854E-5 0.0 0.01021716298345986 2.0112525557991854E-5 34-35 0.0 2.0112525557991854E-5 0.0 0.017638684914358854 3.016878833698778E-5 36-37 0.0 2.0112525557991854E-5 0.0 0.030028000658081835 4.022505111598371E-5 38-39 0.0 2.0112525557991854E-5 0.0 0.04826000507640145 5.028131389497963E-5 40-41 0.0 3.016878833698778E-5 0.0 0.06926753802172395 7.039383945297149E-5 42-43 0.0 4.022505111598371E-5 0.0 0.09011417076258249 8.045010223196742E-5 44-45 0.0 4.022505111598371E-5 0.0 0.11581797842569608 8.045010223196742E-5 46-47 0.0 4.022505111598371E-5 0.0 0.14648957990163367 8.045010223196742E-5 48-49 0.0 4.022505111598371E-5 0.0 0.18014789142293303 8.045010223196742E-5 50-51 0.0 4.022505111598371E-5 0.0 0.21705437582184806 8.045010223196742E-5 52-53 0.0 6.0337576673975555E-5 0.0 0.2549664864986627 8.045010223196742E-5 54-55 0.0 6.0337576673975555E-5 0.0 0.29457810558512765 8.045010223196742E-5 56-57 0.0 6.0337576673975555E-5 0.0 0.3448292506917703 8.045010223196742E-5 58-59 0.0 6.0337576673975555E-5 0.0 0.4038997382555924 8.045010223196742E-5 60-61 0.0 6.0337576673975555E-5 0.0 0.4677771194277745 8.045010223196742E-5 62-63 0.0 6.0337576673975555E-5 0.0 0.5318053445416415 8.045010223196742E-5 64-65 0.0 6.0337576673975555E-5 0.0 0.6006605757894268 8.045010223196742E-5 66-67 0.0 6.0337576673975555E-5 0.0 0.6846504825196007 8.045010223196742E-5 68-69 0.0 6.0337576673975555E-5 0.0 0.7817738684391433 8.045010223196742E-5 70-71 0.0 6.0337576673975555E-5 0.0 0.8900798185689294 8.045010223196742E-5 72-73 0.0 6.0337576673975555E-5 0.0 1.007295617520906 8.045010223196742E-5 74-75 0.0 6.0337576673975555E-5 0.0 1.1245717540495566 8.045010223196742E-5 76-77 0.0 6.0337576673975555E-5 0.0 1.2572741976811868 8.045010223196742E-5 78-79 0.0 6.0337576673975555E-5 0.0 1.4106623738492114 8.045010223196742E-5 80-81 0.0 6.0337576673975555E-5 0.0 1.58444465093304 8.045010223196742E-5 82-83 0.0 6.0337576673975555E-5 0.0 1.76983185526383 8.045010223196742E-5 84-85 0.0 6.0337576673975555E-5 0.0 1.960619272706941 8.045010223196742E-5 86-87 0.0 6.0337576673975555E-5 0.0 2.1634440366965095 8.045010223196742E-5 88 0.0 6.0337576673975555E-5 0.0 2.3345614041439045 8.045010223196742E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11805 0.0 62.1853 1 GTATCAA 23145 0.0 44.396126 1 ATCAACG 29085 0.0 35.079906 3 TCAACGC 29300 0.0 34.8061 4 TATCAAC 29695 0.0 34.44192 2 CAACGCA 30255 0.0 33.73852 5 AACGCAG 31460 0.0 32.477036 6 TGGTATC 4040 0.0 31.077162 2 GTGGTAT 4200 0.0 30.007948 1 ACGCAGA 35085 0.0 29.12149 7 CGCAGAG 35295 0.0 28.948223 8 GCAGAGT 38130 0.0 26.549383 9 TATAACG 380 0.0 24.749012 2 GTACATG 18975 0.0 24.015564 1 GAGTACT 27180 0.0 23.196196 12-13 TACATGG 19025 0.0 23.184067 2 CAGAGTA 37745 0.0 22.599186 10-11 ACATGGG 19265 0.0 22.28279 3 AGAGTAC 36135 0.0 21.947815 10-11 CATGGGT 7165 0.0 21.064552 4 >>END_MODULE