FastQCFastQC Report
Wed 25 May 2016
SRR2431386_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431386_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364531
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA253870.5816661629852097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53500.048.1911161
GTATCAA96450.038.9260981
TCAACGC112450.031.835424
ATCAACG113550.031.694553
TATCAAC121300.030.3676572
CAACGCA121900.029.4060465
GTACATG223850.029.0042861
TACATGG223850.027.7623372
AACGCAG130750.027.280486
CATGGGT97750.027.1423664
ACATGGG229650.026.3032173
CGCAGAG146050.024.0040348
ATGGGTG54850.023.5853025
ACGCAGA150450.023.4584677
GCAGAGT164500.020.511889
CATGGGG102900.020.160844
ATGGGTA43900.019.8240595
TACCTGG36250.018.6880842
TGGTATC27000.018.6436212
GTGGTAT29750.018.502871
Produced by FastQC (version 0.11.5)