##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431386_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4364531 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.687679386399136 31.0 31.0 34.0 26.0 34.0 2 30.828365292857352 33.0 31.0 34.0 27.0 34.0 3 30.90708348732086 34.0 31.0 34.0 27.0 34.0 4 34.13921495803329 37.0 35.0 37.0 30.0 37.0 5 34.20398824066091 37.0 35.0 37.0 30.0 37.0 6 34.279420858736025 37.0 35.0 37.0 31.0 37.0 7 34.19093300059044 37.0 35.0 37.0 30.0 37.0 8 34.165487196677034 37.0 35.0 37.0 30.0 37.0 9 35.59668266762225 39.0 37.0 39.0 30.0 39.0 10-11 35.61202498046182 39.0 37.0 39.0 30.0 39.0 12-13 35.534228534520665 39.0 37.0 39.0 29.5 39.0 14-15 36.88325664315364 41.0 38.0 41.0 29.5 41.0 16-17 36.9101905794689 40.5 38.0 41.0 30.0 41.0 18-19 36.944778373667184 40.5 38.0 41.0 30.0 41.0 20-21 37.028354592967716 41.0 38.0 41.0 30.0 41.0 22-23 37.00911231928471 40.0 38.0 41.0 30.0 41.0 24-25 37.05934108384154 40.5 38.0 41.0 30.0 41.0 26-27 37.096877877600136 40.0 38.0 41.0 30.0 41.0 28-29 37.063299126527 40.0 38.0 41.0 30.0 41.0 30-31 36.99540809768564 40.0 38.0 41.0 30.0 41.0 32-33 36.939486854372205 40.0 38.0 41.0 30.0 41.0 34-35 36.86311816779397 40.0 38.0 41.0 30.0 41.0 36-37 36.73020675073679 40.0 37.0 41.0 30.0 41.0 38-39 36.60905421453073 40.0 37.0 41.0 29.0 41.0 40-41 36.54538677809826 40.0 37.0 41.0 29.0 41.0 42-43 36.385496173586574 40.0 37.0 41.0 28.0 41.0 44-45 36.238211161749106 40.0 36.0 41.0 28.0 41.0 46-47 36.111980989480884 40.0 36.0 41.0 28.0 41.0 48-49 35.979085839921865 40.0 36.0 41.0 28.0 41.0 50-51 35.850492985386055 40.0 35.0 41.0 28.0 41.0 52-53 35.69496161214114 40.0 35.0 41.0 27.0 41.0 54-55 35.536727084765815 39.5 35.0 41.0 27.0 41.0 56-57 35.35336706280698 39.0 35.0 41.0 27.0 41.0 58-59 35.14769903112156 39.0 35.0 41.0 26.5 41.0 60-61 34.921509665070545 39.0 34.0 41.0 26.0 41.0 62-63 34.826864215192884 38.5 34.0 41.0 26.0 41.0 64-65 34.5880408456258 38.0 34.0 40.5 26.0 41.0 66-67 34.33460571135822 37.5 34.0 40.0 26.0 41.0 68-69 34.04379027208192 37.0 34.0 40.0 25.5 41.0 70-71 33.716350164542305 36.5 33.5 40.0 25.0 41.0 72-73 33.372875917252045 36.0 33.0 39.0 24.0 41.0 74-75 33.081697208703524 36.0 33.0 39.0 24.0 41.0 76-77 32.80409498752557 35.0 33.0 39.0 23.5 40.0 78-79 32.44681685157008 35.0 33.0 37.5 22.5 39.5 80-81 32.11138401812245 35.0 33.0 37.0 22.0 39.0 82-83 31.77532270935869 35.0 32.0 36.5 20.5 39.0 84-85 31.466005969484463 35.0 32.0 36.0 20.0 38.0 86-87 31.175112400393076 35.0 32.0 36.0 18.5 37.0 88-89 30.90208512667226 35.0 32.0 35.5 17.5 37.0 90-91 30.66635475839214 35.0 32.0 35.0 13.0 36.5 92-93 30.433147227044557 35.0 31.5 35.0 6.0 36.0 94-95 30.22050490648365 35.0 31.0 35.0 2.0 36.0 96-97 30.019420528803668 35.0 31.0 35.0 2.0 36.0 98-99 29.80723541658886 35.0 31.0 35.0 2.0 35.5 100 29.639350711451012 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1922636493844081 1101 2 0.21941898388873327 1101 3 0.24698365704073666 1101 4 0.30532397203383255 1101 5 0.19087995347936726 1101 6 0.31556213023512214 1101 7 0.2674110627558761 1101 8 0.26250586416323785 1101 9 0.26587251677126034 1101 10-11 0.2536542035196234 1101 12-13 0.1693286667350833 1101 14-15 0.1759743761138708 1101 16-17 0.10379707803718219 1101 18-19 0.041759053864325324 1101 20-21 0.0729896403844208 1101 22-23 0.13209662782290366 1101 24-25 0.1868422794954725 1101 26-27 0.18034300598558417 1101 28-29 0.18507434248620314 1101 30-31 0.17952313553576005 1101 32-33 0.22226034870961087 1101 34-35 0.2531633519277676 1101 36-37 0.15722232408311498 1101 38-39 0.13770861998889927 1101 40-41 0.025958801292865985 1101 42-43 0.1453080042726569 1101 44-45 0.012644192446302327 1101 46-47 0.08103890115947365 1101 48-49 0.12178385869498953 1101 50-51 0.0709144525539287 1101 52-53 0.03739465263183206 1101 54-55 0.025690368493762605 1101 56-57 -0.0332513064508575 1101 58-59 -0.004610123009634037 1101 60-61 -9.803155101550942E-4 1101 62-63 0.08289332502364033 1101 64-65 0.10920220756259624 1101 66-67 0.058894284489561244 1101 68-69 0.024060849613555035 1101 70-71 0.06665516534210525 1101 72-73 0.05336757089217059 1101 74-75 0.04380378900012971 1101 76-77 0.1221922911242217 1101 78-79 0.1352442431810914 1101 80-81 0.08844675508390054 1101 82-83 0.10778651623078872 1101 84-85 0.09822063728980979 1101 86-87 0.10993090576311104 1101 88-89 0.02384526537725762 1101 90-91 -0.013033882769242666 1101 92-93 -0.06274920880411017 1101 94-95 -0.04794923600416112 1101 96-97 -0.004829495060899802 1101 98-99 0.020214026770336346 1101 100 -0.07729982725649975 1102 1 -0.0417339744917804 1102 2 -0.02641042861542786 1102 3 0.03305813951508796 1102 4 0.05274108062037186 1102 5 0.04746137927281069 1102 6 0.007434236743449674 1102 7 -0.0267801379798982 1102 8 0.010163071537938606 1102 9 0.16647425721815523 1102 10-11 0.0788366191588068 1102 12-13 0.030319969506045652 1102 14-15 0.0836696402074324 1102 16-17 0.04536781649655808 1102 18-19 -0.004002385057013669 1102 20-21 0.024885034788574956 1102 22-23 -0.002670460166449118 1102 24-25 0.010022960580052143 1102 26-27 0.04303880340531663 1102 28-29 0.019261169974633674 1102 30-31 0.08021868752480543 1102 32-33 0.10111833647883373 1102 34-35 0.11632672446317827 1102 36-37 0.07357180835358434 1102 38-39 0.033120815282629223 1102 40-41 0.04394057103203153 1102 42-43 0.03520638775166418 1102 44-45 0.0016428066687836917 1102 46-47 -0.09199661754708188 1102 48-49 -0.08857040018444451 1102 50-51 -0.1154870414231084 1102 52-53 -0.18060373033302568 1102 54-55 -0.2697045090015848 1102 56-57 -0.11877533266541462 1102 58-59 -0.19636422039229728 1102 60-61 -0.14144519311893333 1102 62-63 -0.1458765054673279 1102 64-65 -0.136560624181989 1102 66-67 -0.13310367937695133 1102 68-69 -0.13481509741443887 1102 70-71 -0.09934587508597303 1102 72-73 -0.1309097996283839 1102 74-75 -0.08697828750836578 1102 76-77 -0.10911023337606451 1102 78-79 -0.17988111811983742 1102 80-81 -0.16029225701649352 1102 82-83 -0.14910565332509407 1102 84-85 -0.18086568099930034 1102 86-87 -0.22568810854839683 1102 88-89 -0.1944844027725381 1102 90-91 -0.21048083232175685 1102 92-93 -0.1812430614345395 1102 94-95 -0.15626727707225996 1102 96-97 -0.146774056941684 1102 98-99 -0.22551622868840937 1102 100 -0.24676835827873944 1103 1 -0.13584406896170975 1103 2 -0.09254024149144868 1103 3 -0.137747439686418 1103 4 -0.0020621151985977804 1103 5 -0.10123807366783666 1103 6 0.0011279343691938948 1103 7 -0.025204552498792054 1103 8 -0.04314951334905004 1103 9 -0.09374259869335333 1103 10-11 -0.12552404623555447 1103 12-13 -0.11459779269647896 1103 14-15 -0.09233520134816331 1103 16-17 -0.019240537045838835 1103 18-19 -0.05951993017300339 1103 20-21 -0.1731579991897192 1103 22-23 -0.09256526199793313 1103 24-25 -0.07692196621305669 1103 26-27 -0.04118718029228319 1103 28-29 -0.12057747698914767 1103 30-31 -0.08834379527957026 1103 32-33 -0.03940319171812234 1103 34-35 -0.06265078492362619 1103 36-37 -0.14726350655175224 1103 38-39 -0.08009039394345763 1103 40-41 -0.11656627601991687 1103 42-43 -0.20851407658764032 1103 44-45 -0.31383663029858155 1103 46-47 -0.3497613623017486 1103 48-49 -0.30142076124275974 1103 50-51 -0.4009910730532553 1103 52-53 -0.4333562564264639 1103 54-55 -0.3872867481461526 1103 56-57 -0.3878098606751763 1103 58-59 -0.40436497023746654 1103 60-61 -0.40344035835267533 1103 62-63 -0.31312034707563186 1103 64-65 -0.30314251836330186 1103 66-67 -0.22700218614189538 1103 68-69 -0.24834708960475638 1103 70-71 -0.3167927018712007 1103 72-73 -0.32208558874666693 1103 74-75 -0.3070620733837046 1103 76-77 -0.2886616173296659 1103 78-79 -0.27001688772791965 1103 80-81 -0.2902965817199288 1103 82-83 -0.30337110088448327 1103 84-85 -0.31070174046919874 1103 86-87 -0.36409378663721625 1103 88-89 -0.47141387195695117 1103 90-91 -0.4353134265327796 1103 92-93 -0.471246810660368 1103 94-95 -0.5207778640820493 1103 96-97 -0.4615994269689345 1103 98-99 -0.5104374006496322 1103 100 -0.43004943522796424 1104 1 -0.10071341629409147 1104 2 -0.10195971391453185 1104 3 -0.014589406674669902 1104 4 0.04915187390886899 1104 5 -0.024682736107763503 1104 6 0.011648861778994046 1104 7 -0.044189346870020074 1104 8 -0.04941201054143818 1104 9 -0.032010997531315866 1104 10-11 -0.100558011658336 1104 12-13 -0.06350294753609376 1104 14-15 -0.03630849117568857 1104 16-17 -0.033765082213044195 1104 18-19 -7.737280821018544E-4 1104 20-21 -0.08474344017529489 1104 22-23 0.012604567204363093 1104 24-25 0.004114543836230666 1104 26-27 -0.03183238340138672 1104 28-29 0.03027268712354214 1104 30-31 -0.0029113982965327523 1104 32-33 -0.07629549885137976 1104 34-35 -0.05384524058458595 1104 36-37 -0.1471112482100878 1104 38-39 -0.08326003017641881 1104 40-41 -0.1411917948991217 1104 42-43 -0.23340622051144067 1104 44-45 -0.21533802676797364 1104 46-47 -0.32226816556731563 1104 48-49 -0.31562823314499155 1104 50-51 -0.2860476549418465 1104 52-53 -0.35899807265219863 1104 54-55 -0.2202646205777583 1104 56-57 -0.2499170184905708 1104 58-59 -0.3355639091984912 1104 60-61 -0.3676227092601039 1104 62-63 -0.31214894799258985 1104 64-65 -0.35044385314097326 1104 66-67 -0.31499334557747716 1104 68-69 -0.3229885926730063 1104 70-71 -0.37212386617905935 1104 72-73 -0.39469565358039205 1104 74-75 -0.29814786631904866 1104 76-77 -0.31990865765722987 1104 78-79 -0.3428975586430454 1104 80-81 -0.3374824925357238 1104 82-83 -0.3495571318884316 1104 84-85 -0.3577354190484847 1104 86-87 -0.3177677536638903 1104 88-89 -0.34705588919241137 1104 90-91 -0.3678964479877713 1104 92-93 -0.40157318467149494 1104 94-95 -0.34179473104316216 1104 96-97 -0.31020699343449465 1104 98-99 -0.35330517352764446 1104 100 -0.39609296652089654 1105 1 -0.18684874380482341 1105 2 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-0.2501492828979295 1105 60-61 -0.3510813007083513 1105 62-63 -0.25140835714022813 1105 64-65 -0.2878972248910543 1105 66-67 -0.24513448104652724 1105 68-69 -0.19034948418800468 1105 70-71 -0.14088966361115496 1105 72-73 -0.14082771608220668 1105 74-75 -0.17896586276383886 1105 76-77 -0.19608518749860337 1105 78-79 -0.2066171019937144 1105 80-81 -0.2021226363789168 1105 82-83 -0.09576109567526458 1105 84-85 -0.1528198034070627 1105 86-87 -0.23658424632580832 1105 88-89 -0.24118661674734554 1105 90-91 -0.20416820894849508 1105 92-93 -0.20583847705545466 1105 94-95 -0.2557002923105074 1105 96-97 -0.20880903137384976 1105 98-99 -0.28954001906873117 1105 100 -0.19006762868070126 1106 1 -0.1491671325661308 1106 2 -0.15775665753793078 1106 3 -0.1065687488282947 1106 4 0.02100445980437371 1106 5 -0.038680632297435125 1106 6 0.007643816680769078 1106 7 -0.018112740879814737 1106 8 -0.011827749526133857 1106 9 -0.043933200982031906 1106 10-11 -0.02606287357073711 1106 12-13 7.817629043458396E-4 1106 14-15 0.0302183115781105 1106 16-17 0.045281732624872006 1106 18-19 -0.003476400829711679 1106 20-21 -0.02117192212916308 1106 22-23 0.031762870996871584 1106 24-25 0.01835598939552341 1106 26-27 -0.07389572126329114 1106 28-29 -0.03263777011898128 1106 30-31 -0.028625079153300703 1106 32-33 -0.07642377603780659 1106 34-35 -0.03620532361031081 1106 36-37 -0.11876197761099405 1106 38-39 -0.1347371431559523 1106 40-41 -0.08846668511160516 1106 42-43 -0.031756219093161064 1106 44-45 -0.027860751430452524 1106 46-47 -0.03548007357808558 1106 48-49 -0.05038878686008985 1106 50-51 -0.1090352292501322 1106 52-53 -0.13396971207505715 1106 54-55 -0.10646073820864643 1106 56-57 -0.06478581350474855 1106 58-59 -0.17241656501602876 1106 60-61 -0.18813888664110578 1106 62-63 -0.13536976932760325 1106 64-65 -0.06995495576887834 1106 66-67 -0.03978378526114312 1106 68-69 -0.082948528336793 1106 70-71 -0.13061445963169405 1106 72-73 -0.11587000810079218 1106 74-75 -0.12309555124497962 1106 76-77 -0.09796793775975488 1106 78-79 0.003102127154093637 1106 80-81 -0.05163299233448981 1106 82-83 -0.036843596925727695 1106 84-85 -0.04510314985915187 1106 86-87 -0.13384058386402486 1106 88-89 -0.09547434938415833 1106 90-91 -0.06905103192784168 1106 92-93 -0.08388706460275586 1106 94-95 -0.11612917489715358 1106 96-97 -0.07707673899166778 1106 98-99 0.01420570505219132 1106 100 0.008106972622133668 1107 1 -0.5643616764372865 1107 2 -0.5892876498996564 1107 3 -0.5655792068561567 1107 4 -0.46129985499531045 1107 5 -0.47206441429781165 1107 6 -0.5315432884097504 1107 7 -0.5559935515879744 1107 8 -0.47972503757459606 1107 9 -0.5490770468201163 1107 10-11 -0.5358689051596599 1107 12-13 -0.5528005720057436 1107 14-15 -0.6592047321453407 1107 16-17 -0.6255900126688232 1107 18-19 -0.6527365030314201 1107 20-21 -0.6038325905835862 1107 22-23 -0.6486782479277053 1107 24-25 -0.6631871355828949 1107 26-27 -0.5891530861932139 1107 28-29 -0.6667857151510361 1107 30-31 -0.7196234448466754 1107 32-33 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2215 1 0.21587416113746016 2215 2 0.12498091071370254 2215 3 0.12799523870552676 2215 4 0.0392985376772188 2215 5 0.18721383770505184 2215 6 0.11091746333062957 2215 7 0.052949031107360156 2215 8 0.01949597447465834 2215 9 0.017198549635850213 2215 10-11 0.035995805480894205 2215 12-13 0.03847232092808639 2215 14-15 0.09422040427929801 2215 16-17 0.13402809664312798 2215 18-19 0.21976732024042178 2215 20-21 0.15903638120107644 2215 22-23 0.19370802423576805 2215 24-25 0.2322329013108444 2215 26-27 0.13108737784522617 2215 28-29 0.11884063095721586 2215 30-31 0.09811632020840477 2215 32-33 0.08267366040324475 2215 34-35 0.07508505190017445 2215 36-37 0.037673416929116854 2215 38-39 0.14866992696637737 2215 40-41 0.17133811022124945 2215 42-43 0.24781754728577 2215 44-45 0.26594343416078914 2215 46-47 0.27754080967844175 2215 48-49 0.35539107446382445 2215 50-51 0.3404358387939723 2215 52-53 0.3490266973118139 2215 54-55 0.3696847052320038 2215 56-57 0.32575082245921294 2215 58-59 0.29519676523553784 2215 60-61 0.38452093876880866 2215 62-63 0.3587653631999146 2215 64-65 0.3336043223216194 2215 66-67 0.35258547915029936 2215 68-69 0.2723587871774171 2215 70-71 0.3078449016568854 2215 72-73 0.31473974649001235 2215 74-75 0.3450424298649821 2215 76-77 0.36532404166705845 2215 78-79 0.3846844916136831 2215 80-81 0.4282221420943344 2215 82-83 0.40838948955197196 2215 84-85 0.35629933846580997 2215 86-87 0.3435991931348674 2215 88-89 0.35325633078621976 2215 90-91 0.3635918898362043 2215 92-93 0.4320934302763746 2215 94-95 0.4225091956825153 2215 96-97 0.38773229533596165 2215 98-99 0.38925648406240754 2215 100 0.4097281982720169 2216 1 0.25481378027321 2216 2 0.10782741572308652 2216 3 0.0960830852143566 2216 4 0.10987728821795884 2216 5 0.18782112164183218 2216 6 0.06403114287057576 2216 7 0.0900045764623556 2216 8 0.1131732010978439 2216 9 0.0821975282195666 2216 10-11 0.09770304538834296 2216 12-13 0.09081720444789454 2216 14-15 0.13925199241394637 2216 16-17 0.1260776224005511 2216 18-19 0.16612713307311822 2216 20-21 0.07556400205758251 2216 22-23 0.09868869106976774 2216 24-25 0.08291407130402462 2216 26-27 0.08027702749561882 2216 28-29 0.15221951422360291 2216 30-31 0.10598253520790024 2216 32-33 0.10716505270066534 2216 34-35 0.08923397874423955 2216 36-37 0.11988373152330922 2216 38-39 0.1853037005935576 2216 40-41 0.19027244602416715 2216 42-43 0.2330648833599227 2216 44-45 0.33466173843170566 2216 46-47 0.3403392882444649 2216 48-49 0.36727887735334974 2216 50-51 0.4000467470864848 2216 52-53 0.33590143958992513 2216 54-55 0.3500935308611446 2216 56-57 0.3202895934117507 2216 58-59 0.4006125462083432 2216 60-61 0.42037795894820107 2216 62-63 0.4152647987978426 2216 64-65 0.3703193072351638 2216 66-67 0.2792665843490667 2216 68-69 0.30560158034614204 2216 70-71 0.24357374551265565 2216 72-73 0.23305172383266637 2216 74-75 0.241864348013209 2216 76-77 0.2890933036426091 2216 78-79 0.3015214372018562 2216 80-81 0.26229698379039945 2216 82-83 0.22966732521148003 2216 84-85 0.2721550368765584 2216 86-87 0.2914512653034329 2216 88-89 0.3042774750153612 2216 90-91 0.28634996294198345 2216 92-93 0.2600050774481488 2216 94-95 0.23418062014983576 2216 96-97 0.254824108569462 2216 98-99 0.24528129121834397 2216 100 0.24783040416481228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103834.0 3 21293.0 4 15152.0 5 12849.0 6 8758.0 7 7432.0 8 8119.0 9 8724.0 10 8698.0 11 9434.0 12 10757.0 13 11939.0 14 12801.0 15 13345.0 16 13436.0 17 13935.0 18 13612.0 19 12832.0 20 12675.0 21 13290.0 22 15419.0 23 22830.0 24 36143.0 25 48641.0 26 56263.0 27 44440.0 28 39721.0 29 48404.0 30 58917.0 31 66648.0 32 84146.0 33 111878.0 34 156422.0 35 247106.0 36 411264.0 37 754149.0 38 1195781.0 39 630872.0 40 2572.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.933322606729895 21.783918311941715 14.146217070494213 22.136542010834177 2 14.697820924831984 22.606177756825474 40.34316382930769 22.35283748903485 3 17.245390854314568 28.87705493971733 30.809433400521947 23.068120805446156 4 11.653780783527491 19.337523684050463 39.38278589847906 29.62590963394299 5 11.81577021504004 40.61996982699648 34.763597363948314 12.800662594015167 6 27.282238857339138 40.549606999375634 19.100616827200106 13.067537316085131 7 24.45741264521869 35.69229746199063 23.314763837981054 16.53552605480963 8 23.61625062935216 37.81182164091023 21.235534071222947 17.336393658514666 9 24.74036567517304 18.832945429335446 21.717878247664324 34.708810647827185 10-11 22.021012474680795 29.629155341715823 29.358995935628574 18.99083624797481 12-13 22.828412479934478 27.833425850834274 28.848963058805133 20.489198610426115 14-15 20.80776981825156 29.345327535707526 26.372502387685902 23.47440025835501 16-17 19.932845898647518 32.34808935232632 27.078808054823796 20.640256694202368 18-19 19.99870913497943 31.15541705315234 28.935255323585814 19.91061848828242 20-21 21.936774458485317 30.09331160766684 28.09256848990377 19.877345443944076 22-23 21.45738725861768 30.190611270309226 28.183706214596 20.1682952564771 24-25 21.037675154226516 30.219741544195706 28.201888274566073 20.540695027011708 26-27 20.686977217697557 30.181552204857287 28.289458599310297 20.84201197813486 28-29 20.656653566474787 30.231452773103236 28.372835106450665 20.73905855397131 30-31 20.930961510813553 30.555424983381663 28.064062046062656 20.44955145974213 32-33 20.613867495227055 30.39760282457098 28.41748199131933 20.571047688882636 34-35 20.875827689106117 30.07146125325289 28.3523579874829 20.70035307015809 36-37 21.03554842751489 30.166586101666 28.074582858275004 20.723282612544107 38-39 20.826333353550293 30.209541358916926 28.302672915504147 20.661452372028638 40-41 20.72826723118469 29.990868427976658 28.462932295097097 20.817932045741554 42-43 20.691029385361595 30.015968386080903 28.435043132298443 20.857959096259055 44-45 20.893327049888033 30.08891992391069 28.324177619697917 20.693575406503367 46-47 20.874707568061186 30.28267799725181 28.15317156446573 20.68944287022127 48-49 20.824541535675905 29.99696961740474 28.234944886134777 20.94354396078458 50-51 20.70787044388659 29.989985020525832 28.285821896173267 21.0163226394143 52-53 20.7778830788337 30.044317400363813 28.272744970753543 20.905054550048945 54-55 20.90715513493384 30.251731731970448 28.123091013154927 20.718022119940784 56-57 20.873689403412502 30.047971563982127 27.988506452136946 21.08983258046842 58-59 20.84390025945365 29.80792128770829 28.28043650219009 21.06774195064797 60-61 21.009320665470934 30.128372401034426 27.972406721115178 20.889900212379462 62-63 20.80376529608544 30.050958291011394 28.18397032152446 20.961306091378706 64-65 20.873045225642116 29.88531654143286 28.23514683320994 21.006491399715085 66-67 20.694632749124512 30.000071080001 28.067635143144802 21.237661027729686 68-69 21.08459177510796 30.105511005088797 27.903977350111642 20.905919869691605 70-71 21.021465462254195 29.83744421093757 28.034127108956103 21.106963217852133 72-73 20.86547490232146 29.990598694959917 28.25177568424271 20.892150718475907 74-75 20.870989368570882 30.04035623358328 28.137874282800418 20.95078011504542 76-77 20.921057534281125 30.03556948144225 27.977572596423105 21.06580038785352 78-79 20.984814413094877 29.988023174990158 28.194365515456894 20.83279689645807 80-81 20.849591012736482 29.95643675693921 28.209928603793244 20.984043626531065 82-83 20.742922809850896 29.90286896396428 28.44626458206665 20.907943644118177 84-85 20.772425533905523 29.85671061938349 28.18941353432425 21.18145031238674 86-87 21.038171548281216 29.918060510114252 28.04890045294418 20.994867488660343 88-89 20.845640427914265 29.760658587877415 28.217090383852938 21.17661060035538 90-91 20.795816298897115 29.73989565282793 28.1650802255161 21.299207822758856 92-93 20.92598844764267 29.828273755444723 28.105302196117226 21.140435600795378 94-95 20.80040500482697 29.78021311590615 28.21645976393749 21.20292211532939 96-97 20.798345049593483 29.887675228019976 28.088724876532172 21.22525484585437 98-99 20.757292099118136 29.86133922000459 28.100806722985823 21.280561957891454 100 20.84337371500696 29.935143797222665 27.940796069643483 21.280686418126898 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 23448.0 1 17359.5 2 9885.0 3 7141.5 4 4801.0 5 3409.5 6 2871.0 7 2789.5 8 3062.0 9 3155.0 10 2489.5 11 1871.0 12 1810.0 13 1869.0 14 1910.5 15 2013.0 16 2292.5 17 2788.0 18 3579.0 19 4664.5 20 6367.5 21 8973.5 22 13274.0 23 20395.5 24 28091.5 25 35966.0 26 46997.0 27 60045.0 28 77220.0 29 93598.0 30 102286.0 31 111276.0 32 121179.5 33 130253.0 34 139154.5 35 145919.5 36 149119.5 37 150192.0 38 150096.5 39 148681.0 40 147617.0 41 144318.5 42 138545.0 43 133989.0 44 131093.0 45 127975.5 46 126123.5 47 124699.0 48 121140.0 49 117741.0 50 115491.0 51 110952.5 52 104281.0 53 98667.0 54 95693.5 55 93735.0 56 87094.5 57 78056.5 58 71109.5 59 64549.5 60 56673.5 61 47935.0 62 40336.5 63 33089.0 64 26379.0 65 21314.5 66 17049.0 67 13255.0 68 10328.5 69 7933.0 70 5948.0 71 4529.5 72 3544.5 73 2803.0 74 2231.5 75 1764.5 76 1308.5 77 1006.0 78 808.0 79 592.5 80 434.0 81 304.5 82 231.0 83 186.5 84 160.0 85 144.0 86 136.0 87 107.5 88 84.0 89 70.5 90 57.0 91 50.0 92 37.5 93 29.0 94 25.0 95 29.5 96 28.5 97 24.0 98 30.0 99 32.0 100 37.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17784270520704287 2 0.17287080788290884 3 0.18645760563964375 4 0.17633051523749058 5 0.19060467207129472 6 0.07657180118551112 7 0.07872552629366134 8 0.06651344669106485 9 0.060189743182028035 10-11 0.07370780503105602 12-13 0.0731808297386363 14-15 0.07080944092274749 16-17 0.06692586213730639 18-19 0.07135932818440285 20-21 0.07277987027701258 22-23 0.07077507296889403 24-25 0.07077507296889403 26-27 0.0744868119850678 28-29 0.07204668726147208 30-31 0.07753410389340802 32-33 0.07404002858497283 34-35 0.07369634904643821 36-37 0.08392654331015177 38-39 0.080340820124774 40-41 0.07534601083140434 42-43 0.07822146297047725 44-45 0.08857767306498682 46-47 0.07958472513999786 48-49 0.08467118231031008 50-51 0.08164680237120552 52-53 0.07996277263238592 54-55 0.08200193789435795 56-57 0.07564386643146766 58-59 0.08331937612540728 60-61 0.07948162127843748 62-63 0.07150825598443453 64-65 0.07382236487723423 66-67 0.0744180760773609 68-69 0.07297462201551552 70-71 0.06903376330698534 72-73 0.06650199070644704 74-75 0.0715540799229058 76-77 0.07672072898554277 78-79 0.0731235498155472 80-81 0.08053557186327695 82-83 0.08171553827891245 84-85 0.08409838307941907 86-87 0.08037518807862747 88-89 0.08165825835582334 90-91 0.08046683595557003 92-93 0.070649057138098 94-95 0.07106147258433954 96-97 0.07036265752265249 98-99 0.08002005255547504 100 0.08722586688008402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4364531.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.16018537189968 #Duplication Level Percentage of deduplicated Percentage of total 1 75.2998787015477 46.053545398682175 2 15.240499084223613 18.64223498302767 3 4.671805487446328 8.571854689010266 4 1.882735908396848 4.605939086555326 5 0.9002922537079245 2.7531020562831 6 0.5124939905445841 1.8806536478214817 7 0.33380545725731736 1.4290922550806484 8 0.22354218584380844 1.0937505219717578 9 0.15861067845853857 0.8730592646838296 >10 0.7042364668872996 7.452009734403241 >50 0.043480304774323034 1.8423631949779669 >100 0.026335183131619134 3.0214383163331755 >500 0.0016476904903567206 0.6610024568163988 >1k 5.99159802030946E-4 0.5385171303562011 >5k 0.0 0.0 >10k+ 3.74474876269284E-5 0.5814372639965678 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25387 0.5816661629852097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.2911969235640668E-5 0.0 3 0.0 0.0 0.0 2.2911969235640668E-5 0.0 4 0.0 0.0 0.0 2.2911969235640668E-5 0.0 5 2.2911969235640668E-5 0.0 0.0 2.2911969235640668E-5 0.0 6 2.2911969235640668E-5 0.0 0.0 2.2911969235640668E-5 0.0 7 2.2911969235640668E-5 0.0 0.0 2.2911969235640668E-5 0.0 8 2.2911969235640668E-5 0.0 0.0 2.2911969235640668E-5 0.0 9 1.1455984617820334E-4 0.0 0.0 4.5823938471281337E-5 0.0 10-11 1.2601583079602368E-4 0.0 0.0 6.873590770692201E-5 0.0 12-13 1.6038378464948468E-4 0.0 0.0 6.873590770692201E-5 0.0 14-15 1.8329575388512535E-4 0.0 0.0 9.164787694256267E-5 0.0 16-17 1.8329575388512535E-4 0.0 0.0 1.03103861560383E-4 2.2911969235640668E-5 18-19 1.9475173850294568E-4 0.0 0.0 1.2601583079602368E-4 2.2911969235640668E-5 20-21 2.06207723120766E-4 0.0 0.0 2.1766370773858637E-4 2.2911969235640668E-5 22-23 2.06207723120766E-4 0.0 0.0 4.696953693306337E-4 2.2911969235640668E-5 24-25 2.06207723120766E-4 0.0 0.0 6.873590770692201E-4 2.2911969235640668E-5 26-27 2.06207723120766E-4 0.0 0.0 0.0011799664156354945 2.2911969235640668E-5 28-29 2.06207723120766E-4 0.0 0.0 0.0025088606313026533 2.2911969235640668E-5 30-31 2.06207723120766E-4 0.0 0.0 0.005957112001266574 2.2911969235640668E-5 32-33 2.06207723120766E-4 0.0 0.0 0.012876526710430054 2.2911969235640668E-5 34-35 2.2911969235640668E-4 0.0 0.0 0.0226484815894308 2.2911969235640668E-5 36-37 2.2911969235640668E-4 0.0 0.0 0.038125516808106066 2.2911969235640668E-5 38-39 2.2911969235640668E-4 0.0 0.0 0.060544878705180466 2.2911969235640668E-5 40-41 2.2911969235640668E-4 0.0 0.0 0.0837776155101201 2.2911969235640668E-5 42-43 2.2911969235640668E-4 0.0 0.0 0.10887767780776446 2.2911969235640668E-5 44-45 2.5203166159204737E-4 2.2911969235640668E-5 0.0 0.14030144361444563 2.2911969235640668E-5 46-47 2.5203166159204737E-4 3.4367953853461004E-5 0.0 0.17918305540732785 2.2911969235640668E-5 48-49 2.5203166159204737E-4 4.5823938471281337E-5 0.0 0.22253250120115997 2.2911969235640668E-5 50-51 2.634876462098677E-4 4.5823938471281337E-5 0.0 0.26723375317989495 2.2911969235640668E-5 52-53 2.7494363082768803E-4 4.5823938471281337E-5 0.0 0.310331167312135 4.5823938471281337E-5 54-55 2.7494363082768803E-4 4.5823938471281337E-5 0.0 0.3585952305070121 4.5823938471281337E-5 56-57 2.978556000633287E-4 4.5823938471281337E-5 0.0 0.4199305721508222 4.5823938471281337E-5 58-59 3.2076756929896936E-4 4.5823938471281337E-5 0.0 0.4880020327499106 4.5823938471281337E-5 60-61 3.2076756929896936E-4 4.5823938471281337E-5 0.0 0.5619504134579408 4.5823938471281337E-5 62-63 3.5513552315243036E-4 4.5823938471281337E-5 0.0 0.6382243590433886 4.5823938471281337E-5 64-65 3.665915077702507E-4 4.5823938471281337E-5 0.0 0.7207532722301664 4.5823938471281337E-5 66-67 3.665915077702507E-4 4.5823938471281337E-5 0.0 0.821394097097718 4.5823938471281337E-5 68-69 3.665915077702507E-4 4.5823938471281337E-5 0.0 0.937053717799232 4.5823938471281337E-5 70-71 3.665915077702507E-4 4.5823938471281337E-5 0.0 1.0688089968887837 5.7279923089101676E-5 72-73 3.665915077702507E-4 6.873590770692201E-5 0.0 1.2057194690563544 6.873590770692201E-5 74-75 3.8950347700589136E-4 6.873590770692201E-5 0.0 1.3455970412399407 6.873590770692201E-5 76-77 3.8950347700589136E-4 6.873590770692201E-5 0.0 1.4997373142727133 6.873590770692201E-5 78-79 3.8950347700589136E-4 8.019189232474234E-5 0.0 1.6828612283885716 6.873590770692201E-5 80-81 3.8950347700589136E-4 9.164787694256267E-5 0.0 1.8877744252475237 6.873590770692201E-5 82-83 4.12415446241532E-4 9.164787694256267E-5 0.0 2.1080730094482085 6.873590770692201E-5 84-85 4.12415446241532E-4 9.164787694256267E-5 0.0 2.330456582849337 6.873590770692201E-5 86-87 4.12415446241532E-4 9.164787694256267E-5 0.0 2.5683171914691405 6.873590770692201E-5 88 4.12415446241532E-4 9.164787694256267E-5 0.0 2.7637104651106843 6.873590770692201E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5350 0.0 48.191116 1 GTATCAA 9645 0.0 38.926098 1 TCAACGC 11245 0.0 31.83542 4 ATCAACG 11355 0.0 31.69455 3 TATCAAC 12130 0.0 30.367657 2 CAACGCA 12190 0.0 29.406046 5 GTACATG 22385 0.0 29.004286 1 TACATGG 22385 0.0 27.762337 2 AACGCAG 13075 0.0 27.28048 6 CATGGGT 9775 0.0 27.142366 4 ACATGGG 22965 0.0 26.303217 3 CGCAGAG 14605 0.0 24.004034 8 ATGGGTG 5485 0.0 23.585302 5 ACGCAGA 15045 0.0 23.458467 7 GCAGAGT 16450 0.0 20.51188 9 CATGGGG 10290 0.0 20.16084 4 ATGGGTA 4390 0.0 19.824059 5 TACCTGG 3625 0.0 18.688084 2 TGGTATC 2700 0.0 18.643621 2 GTGGTAT 2975 0.0 18.50287 1 >>END_MODULE