FastQCFastQC Report
Wed 25 May 2016
SRR2431386_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431386_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4364531
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336540.771079412656251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278930.6390835578897252No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182290.41766228719649373No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67910.15559518307923578No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57240.13114811190480719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56510.12947553815060542No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50500.11570544463998537No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46430.10638027316107962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121350.062.27121
GTATCAA243900.045.4999921
GTGGTAT42200.038.888761
TGGTATC41750.037.7285042
ATCAACG316000.034.802023
TATCAAC318300.034.5669062
TCAACGC318400.034.4954
CAACGCA328450.033.4538275
AACGCAG343300.032.1851846
CGCAGAG382200.028.749558
ACGCAGA384250.028.632867
GCAGAGT413750.026.3755449
GTACATG213850.025.8588471
TATAACG4500.025.0772532
CATGGGT98950.025.0409724
TACATGG217600.024.8712562
ACATGGG216450.024.3071373
ATAACGC5600.023.5088483
GAGTACT288250.023.09534612-13
CAGAGTA407750.022.30881110-11
Produced by FastQC (version 0.11.5)