##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431386_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4364531 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.62707379097548 34.0 31.0 34.0 31.0 34.0 2 32.74960219093415 34.0 31.0 34.0 31.0 34.0 3 32.85834377164465 34.0 31.0 34.0 31.0 34.0 4 36.32770164766844 37.0 37.0 37.0 35.0 37.0 5 36.22681841416638 37.0 37.0 37.0 35.0 37.0 6 36.270926475261604 37.0 37.0 37.0 35.0 37.0 7 36.22988953452273 37.0 37.0 37.0 35.0 37.0 8 36.24149696725719 37.0 37.0 37.0 35.0 37.0 9 38.03828223467768 39.0 39.0 39.0 35.0 39.0 10-11 38.020205034630294 39.0 39.0 39.0 35.0 39.0 12-13 37.997631933419655 39.0 39.0 39.0 35.0 39.0 14-15 39.46326443780558 41.0 39.0 41.0 36.0 41.0 16-17 39.49253241642687 41.0 39.0 41.0 36.0 41.0 18-19 39.566851512797136 41.0 40.0 41.0 36.5 41.0 20-21 39.56358460966367 41.0 40.0 41.0 37.0 41.0 22-23 39.501191995199484 41.0 39.5 41.0 36.0 41.0 24-25 39.44926247516629 41.0 39.0 41.0 36.0 41.0 26-27 39.332843666364155 41.0 39.0 41.0 36.0 41.0 28-29 39.24946620839673 41.0 39.0 41.0 35.5 41.0 30-31 39.06799848597707 41.0 39.0 41.0 35.0 41.0 32-33 38.839684836698375 41.0 38.0 41.0 35.0 41.0 34-35 38.700558318866335 41.0 38.0 41.0 35.0 41.0 36-37 38.554490734514204 41.0 38.0 41.0 35.0 41.0 38-39 38.40631731106962 41.0 38.0 41.0 35.0 41.0 40-41 38.266448330874496 40.0 38.0 41.0 34.5 41.0 42-43 38.09326030677752 40.0 38.0 41.0 34.0 41.0 44-45 37.8456912094335 40.0 38.0 41.0 33.5 41.0 46-47 37.46986835469836 40.0 38.0 41.0 33.0 41.0 48-49 37.129010425175125 40.0 37.0 41.0 32.0 41.0 50-51 36.96358062298103 40.0 37.0 41.0 31.0 41.0 52-53 36.90302841244569 40.0 37.0 41.0 31.0 41.0 54-55 36.76231352234639 40.0 36.5 41.0 31.0 41.0 56-57 36.61843643681303 40.0 36.0 41.0 31.0 41.0 58-59 36.44350607201552 40.0 36.0 41.0 31.0 41.0 60-61 36.29371437618383 40.0 35.0 41.0 31.0 41.0 62-63 36.13602744487323 39.0 35.0 41.0 31.0 41.0 64-65 35.90750690051233 39.0 35.0 41.0 30.0 41.0 66-67 35.64706070365865 39.0 35.0 41.0 30.0 41.0 68-69 35.35866866336841 38.0 35.0 41.0 29.5 41.0 70-71 35.06253466867345 37.0 35.0 40.0 29.0 41.0 72-73 34.74746759732031 37.0 35.0 39.5 29.0 41.0 74-75 34.41080553672319 36.0 35.0 39.0 29.0 41.0 76-77 34.07656275095766 36.0 35.0 39.0 29.0 41.0 78-79 33.755654845847126 35.5 35.0 38.0 28.5 40.0 80-81 33.48869958765329 35.0 34.0 37.0 29.0 39.0 82-83 33.28280621675044 35.0 34.0 37.0 29.0 39.0 84-85 33.015175170023994 35.0 34.0 36.5 29.0 38.5 86-87 32.76923167689724 35.0 34.0 36.0 29.0 37.0 88-89 32.53409484318017 35.0 34.0 36.0 28.5 37.0 90-91 32.34937430848813 35.0 34.0 35.5 28.0 36.5 92-93 32.19003588243502 35.0 34.0 35.0 27.0 36.0 94-95 32.05025007268822 35.0 34.0 35.0 27.0 36.0 96-97 31.90750999362818 35.0 34.0 35.0 27.0 36.0 98-99 31.792240449202904 35.0 34.0 35.0 26.5 36.0 100 31.689931403855304 35.0 34.0 35.0 26.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04040689870583236 1101 2 -0.013844768962947285 1101 3 -0.031430650530161586 1101 4 0.046859236563889795 1101 5 0.0046002765715584815 1101 6 -0.020060403477110356 1101 7 0.023670455119756184 1101 8 0.03913348666954164 1101 9 0.042885540721705695 1101 10-11 0.06483083508589971 1101 12-13 0.030580214008999462 1101 14-15 0.018260427256436174 1101 16-17 0.004068546357679281 1101 18-19 -0.03701602719823427 1101 20-21 0.028312865449194646 1101 22-23 0.012279196213732746 1101 24-25 0.04812983071382604 1101 26-27 0.06858324396144155 1101 28-29 0.018666467517611807 1101 30-31 -0.009749027626149598 1101 32-33 0.01084310810060174 1101 34-35 0.026068258508118447 1101 36-37 0.012488067950037873 1101 38-39 0.0058116420009568515 1101 40-41 -0.058257791530621716 1101 42-43 -0.05036202627506725 1101 44-45 -0.08401405709435039 1101 46-47 -0.08761467102365117 1101 48-49 -0.10768709957286404 1101 50-51 -0.16620778084622145 1101 52-53 -0.10224190951968382 1101 54-55 -0.1109466190737578 1101 56-57 -0.0913177253806623 1101 58-59 -0.12196737650773315 1101 60-61 -0.15650878812922997 1101 62-63 -0.14251268013769902 1101 64-65 -0.14224105212414173 1101 66-67 -0.2001361393413177 1101 68-69 -0.18990827529678 1101 70-71 -0.17093942361397296 1101 72-73 -0.19286348161450206 1101 74-75 -0.22942500668020926 1101 76-77 -0.18053396066410698 1101 78-79 -0.19899434319869158 1101 80-81 -0.14485621718868202 1101 82-83 -0.13181038900474817 1101 84-85 -0.09918597661148709 1101 86-87 -0.13686598821501406 1101 88-89 -0.07885529500008204 1101 90-91 -0.10599832305193502 1101 92-93 -0.13130160105326638 1101 94-95 -0.19174454530760698 1101 96-97 -0.14980593301687506 1101 98-99 -0.17023046703275924 1101 100 -0.1507409859360216 1102 1 -0.07167450197177061 1102 2 -0.034864049526760255 1102 3 -0.02043360372348957 1102 4 0.0190259491275242 1102 5 -0.01114189051832426 1102 6 -0.03359502733552944 1102 7 -0.009187986241933288 1102 8 -0.011635709488366786 1102 9 -0.0063850374732297155 1102 10-11 -0.021683115766315098 1102 12-13 -0.04926996173564646 1102 14-15 -0.05299426083834646 1102 16-17 -0.07217409476815817 1102 18-19 -0.07787427944064262 1102 20-21 -0.04398536553743071 1102 22-23 -0.03589877743464598 1102 24-25 -0.05471690413171615 1102 26-27 -0.03853205887057243 1102 28-29 -0.131180162784581 1102 30-31 -0.1276603564178842 1102 32-33 -0.06407898969032999 1102 34-35 -0.04290494702750891 1102 36-37 -0.038435222881062714 1102 38-39 -0.0674135781433236 1102 40-41 -0.04832418703382757 1102 42-43 -0.03310147782207906 1102 44-45 -0.06875690261428247 1102 46-47 -0.1701008463688396 1102 48-49 -0.10884073534887051 1102 50-51 -0.14662168003611953 1102 52-53 -0.10124136188444055 1102 54-55 -0.04123480916496902 1102 56-57 -0.014633406946330751 1102 58-59 -0.04093221286228044 1102 60-61 -0.07038605194646408 1102 62-63 -0.1110635337873731 1102 64-65 -0.08956742861368383 1102 66-67 -0.024707630605341535 1102 68-69 -0.030175782778918858 1102 70-71 -0.06208530881829688 1102 72-73 0.04043367730627523 1102 74-75 0.008750053192827068 1102 76-77 -0.013440723743165961 1102 78-79 -0.0317888235054653 1102 80-81 -0.01635867004019076 1102 82-83 -0.047644841515989356 1102 84-85 -0.045103983011351545 1102 86-87 -0.05364743811625772 1102 88-89 -0.05461942245113249 1102 90-91 -0.054133149103684275 1102 92-93 -0.003882485386668577 1102 94-95 0.01984612983869738 1102 96-97 -0.003371706061390256 1102 98-99 0.02084534991315934 1102 100 0.05163410957914749 1103 1 -0.08307238018367968 1103 2 -0.09205291793626458 1103 3 -0.043802978766663614 1103 4 0.023343405206134094 1103 5 -0.004026982835597437 1103 6 -0.0055416525419502705 1103 7 -0.06048939005165721 1103 8 -0.03128358185389857 1103 9 -0.006235025333239719 1103 10-11 -0.006826310005422442 1103 12-13 -0.001296386503426561 1103 14-15 -0.06142015823586178 1103 16-17 -0.007348563089905724 1103 18-19 -0.043461028432936644 1103 20-21 -0.041491844779443454 1103 22-23 -0.021415722286540984 1103 24-25 -0.013580493200187504 1103 26-27 -0.008795533317695003 1103 28-29 -0.08206104764929734 1103 30-31 -0.0895409461078458 1103 32-33 -0.16481569801662488 1103 34-35 -0.08617738750194803 1103 36-37 -0.04283171283407228 1103 38-39 -0.09291468550664916 1103 40-41 -0.10207349907405927 1103 42-43 -0.07089128171676862 1103 44-45 -0.11920438494824737 1103 46-47 -0.11643854298631595 1103 48-49 -0.15041790123805043 1103 50-51 -0.21551653348816302 1103 52-53 -0.10205699901196397 1103 54-55 -0.11905954746006131 1103 56-57 -0.10460491218344714 1103 58-59 -0.09362299365715643 1103 60-61 -0.20653532437142275 1103 62-63 -0.23385187851224742 1103 64-65 -0.1515746557071651 1103 66-67 -0.13819270358915503 1103 68-69 -0.14268294796006842 1103 70-71 -0.15981534871699665 1103 72-73 -0.2176328429476797 1103 74-75 -0.16938541606766933 1103 76-77 -0.15922775079675233 1103 78-79 -0.18207168853042788 1103 80-81 -0.15130552834737188 1103 82-83 -0.13412847020188678 1103 84-85 -0.09872234426019588 1103 86-87 -0.11322436346873133 1103 88-89 -0.15340507040604479 1103 90-91 -0.13438591255469134 1103 92-93 -0.14903485709261588 1103 94-95 -0.1507158682281684 1103 96-97 -0.11300404268690656 1103 98-99 -0.1194504057024055 1103 100 -0.11883957315099636 1104 1 -0.14063318614810782 1104 2 -0.06899148967705315 1104 3 -0.07146852176285279 1104 4 -0.05758585179612652 1104 5 -0.056228947802267726 1104 6 -0.09772197215472289 1104 7 -0.06652297110206717 1104 8 -0.10902375427951938 1104 9 -0.08859471453983048 1104 10-11 -0.12164750376093991 1104 12-13 -0.10801461712496518 1104 14-15 -0.10697564522186553 1104 16-17 -0.10654913435432434 1104 18-19 -0.05245695769881564 1104 20-21 -0.049939996434972045 1104 22-23 -0.09261834050871443 1104 24-25 -0.01817844711955985 1104 26-27 -0.0879303347216478 1104 28-29 -0.13351243707551674 1104 30-31 -0.0473926523045094 1104 32-33 -0.030836084475311054 1104 34-35 -0.09970333201010817 1104 36-37 -0.07418929545872999 1104 38-39 -0.1783454509172273 1104 40-41 -0.16325451632111054 1104 42-43 -0.19643365555914727 1104 44-45 -0.15429715902970997 1104 46-47 -0.2184114169064344 1104 48-49 -0.2104561520837862 1104 50-51 -0.3159877733819414 1104 52-53 -0.23721708785722484 1104 54-55 -0.21064256797527037 1104 56-57 -0.295548646062862 1104 58-59 -0.23747895906719663 1104 60-61 -0.25885679875739953 1104 62-63 -0.2846283582022835 1104 64-65 -0.3034225720612227 1104 66-67 -0.33742735774956856 1104 68-69 -0.40180818913258065 1104 70-71 -0.3127158642521266 1104 72-73 -0.2913083376482817 1104 74-75 -0.29881201572134586 1104 76-77 -0.3313045159414756 1104 78-79 -0.3370648719512275 1104 80-81 -0.28022074098276306 1104 82-83 -0.28055383591689775 1104 84-85 -0.28034769794594894 1104 86-87 -0.26871813942443623 1104 88-89 -0.2651107308712781 1104 90-91 -0.16962520184277707 1104 92-93 -0.1658831269105292 1104 94-95 -0.18072288738339992 1104 96-97 -0.15936051427598485 1104 98-99 -0.17262564932358515 1104 100 -0.08935074140804389 1105 1 -0.16192180595444938 1105 2 -0.13665768237579812 1105 3 -0.12487322660008005 1105 4 -0.06880948497668982 1105 5 -0.10510105351848154 1105 6 -0.024480560924438066 1105 7 -0.0904058512207655 1105 8 -0.020827028098374 1105 9 -0.031416791737996164 1105 10-11 -0.0805680933362396 1105 12-13 -0.08555553224400825 1105 14-15 -0.1213060003982207 1105 16-17 -0.11441491151339989 1105 18-19 -0.10243172750743668 1105 20-21 -0.08822863667934655 1105 22-23 -0.09722012430545846 1105 24-25 -0.15950491665602584 1105 26-27 -0.1141004094423721 1105 28-29 -0.11726514590945669 1105 30-31 -0.13899242669899792 1105 32-33 -0.2180482302069322 1105 34-35 -0.2298017126190075 1105 36-37 -0.24804029350163148 1105 38-39 -0.19829713081636413 1105 40-41 -0.18869174125649124 1105 42-43 -0.2583471107345261 1105 44-45 -0.1792683077504904 1105 46-47 -0.28649646251817273 1105 48-49 -0.28651306225554407 1105 50-51 -0.28979765414690917 1105 52-53 -0.30592997488042073 1105 54-55 -0.28975721231462614 1105 56-57 -0.23283621578546843 1105 58-59 -0.22775132707584334 1105 60-61 -0.2307341166820578 1105 62-63 -0.28056113710189834 1105 64-65 -0.3365896548303411 1105 66-67 -0.2985306921081943 1105 68-69 -0.20037378134642125 1105 70-71 -0.250966370411021 1105 72-73 -0.19283387878986957 1105 74-75 -0.24942725790516107 1105 76-77 -0.2554456516644237 1105 78-79 -0.20172267375477304 1105 80-81 -0.1072399719106798 1105 82-83 -0.17348637374917075 1105 84-85 -0.18474606610160293 1105 86-87 -0.18771037385715772 1105 88-89 -0.14241071829109586 1105 90-91 -0.1628266839943393 1105 92-93 -0.19572788538428654 1105 94-95 -0.1879692776253421 1105 96-97 -0.1703848321182555 1105 98-99 -0.16554375358222373 1105 100 -0.10114968642425026 1106 1 -0.12045140471705906 1106 2 -0.08987499579220781 1106 3 -0.10999665591160834 1106 4 -0.04237649430329071 1106 5 -0.011701998658494972 1106 6 -0.06005184588444479 1106 7 -0.051734658337714734 1106 8 -0.006721712496492671 1106 9 -0.05683617428189791 1106 10-11 -0.09379133230297754 1106 12-13 -0.07680374991118555 1106 14-15 -0.0768706743897738 1106 16-17 -0.054026724093063194 1106 18-19 -0.08791716323192134 1106 20-21 -0.08243359832050601 1106 22-23 -0.09316575320485043 1106 24-25 -0.020395707191262602 1106 26-27 -0.02585759060993098 1106 28-29 -0.03235743905812427 1106 30-31 -0.10122427834994596 1106 32-33 -0.012853264369738326 1106 34-35 -0.02199158179503513 1106 36-37 0.007120303161002539 1106 38-39 0.021715101072139475 1106 40-41 -0.06670780215110028 1106 42-43 -0.08377754840962837 1106 44-45 -0.011598056273271595 1106 46-47 -0.04822017120514488 1106 48-49 8.318956710908765E-4 1106 50-51 -0.07860661853170114 1106 52-53 -0.08341886570190837 1106 54-55 -0.08109701560207583 1106 56-57 -0.07905532406384452 1106 58-59 -0.05189100448220074 1106 60-61 -0.0325369260611339 1106 62-63 -0.07487811178907577 1106 64-65 -0.03035063011892447 1106 66-67 -0.03344419676167121 1106 68-69 -0.03049980001534891 1106 70-71 -0.0544074028325241 1106 72-73 -0.0135695707477268 1106 74-75 -6.998140578602374E-4 1106 76-77 -0.002727145767856598 1106 78-79 -0.007617245144878382 1106 80-81 -0.03807752339073289 1106 82-83 -0.022775418261552716 1106 84-85 -0.03150654415495069 1106 86-87 -0.026522753049142977 1106 88-89 -0.07671386915088618 1106 90-91 -0.023996935962884436 1106 92-93 -0.015019138466207949 1106 94-95 -0.05683090374179045 1106 96-97 -0.09516933076068668 1106 98-99 -0.0736449291603094 1106 100 0.026272787625671867 1107 1 -0.27509958760594344 1107 2 -0.23796322149532045 1107 3 -0.1929686003144937 1107 4 -0.20726009791104616 1107 5 -0.2226737205501479 1107 6 -0.21900773111468652 1107 7 -0.1554064772903061 1107 8 -0.10021325898791389 1107 9 -0.1588414608418347 1107 10-11 -0.15713989614643253 1107 12-13 -0.16174242504862235 1107 14-15 -0.21526983076529405 1107 16-17 -0.22589487851585943 1107 18-19 -0.21662202318411516 1107 20-21 -0.22986901251748293 1107 22-23 -0.20085212554287324 1107 24-25 -0.20541422741485604 1107 26-27 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62-63 0.23290253010405593 2214 64-65 0.1899871805320501 2214 66-67 0.20480861538816697 2214 68-69 0.21773203622992554 2214 70-71 0.22278446698060606 2214 72-73 0.22775913297824246 2214 74-75 0.21839376957564838 2214 76-77 0.22098119698339502 2214 78-79 0.25498733380611327 2214 80-81 0.1766159895482744 2214 82-83 0.16557075149178502 2214 84-85 0.18296334335905584 2214 86-87 0.15368924225072078 2214 88-89 0.1734476581386275 2214 90-91 0.18672217607585395 2214 92-93 0.1483172727224371 2214 94-95 0.12289260225602305 2214 96-97 0.18924914695131534 2214 98-99 0.19491674256397573 2214 100 0.2445501985722558 2215 1 0.1278456392323406 2215 2 0.1075414208945702 2215 3 0.1083050043829914 2215 4 0.050128637898417594 2215 5 0.07380647059574841 2215 6 0.05313368255801265 2215 7 0.059653320686791744 2215 8 0.07806845313004374 2215 9 0.0398540258295057 2215 10-11 0.06866736573028476 2215 12-13 0.053123112574773756 2215 14-15 0.09630138158054535 2215 16-17 0.07547761610561565 2215 18-19 0.06648328362336997 2215 20-21 0.07377295621289193 2215 22-23 0.03195590197131537 2215 24-25 0.09275268627181532 2215 26-27 0.04422194887619213 2215 28-29 0.07346753315015775 2215 30-31 0.10247688192191617 2215 32-33 0.10379512303369864 2215 34-35 0.08557964929278228 2215 36-37 0.048939375717893085 2215 38-39 0.13834094188677426 2215 40-41 0.10457343903979677 2215 42-43 0.07176054698361156 2215 44-45 0.06730817761850716 2215 46-47 0.1757530087421202 2215 48-49 0.16348858462621507 2215 50-51 0.18487565135025363 2215 52-53 0.12402910086334629 2215 54-55 0.16900985444191008 2215 56-57 0.148004495695659 2215 58-59 0.14158830758635332 2215 60-61 0.13949235625221945 2215 62-63 0.19902784351657488 2215 64-65 0.14017862528017133 2215 66-67 0.12678006742863346 2215 68-69 0.11023525970202996 2215 70-71 0.11345583648507329 2215 72-73 0.0877419717993817 2215 74-75 0.10715223617074798 2215 76-77 0.09853593932005822 2215 78-79 0.18141325704516476 2215 80-81 0.20569519141214698 2215 82-83 0.18822450170395655 2215 84-85 0.18510874540699263 2215 86-87 0.20792385843859051 2215 88-89 0.15117439019108048 2215 90-91 0.1680915014381057 2215 92-93 0.1422775121573565 2215 94-95 0.11318977458866897 2215 96-97 0.11890055516537856 2215 98-99 0.10172777028099134 2215 100 0.17900869223022653 2216 1 0.10907789648000943 2216 2 0.09482350582666754 2216 3 0.0747675338244207 2216 4 0.03625974889765615 2216 5 0.03569109378823754 2216 6 0.031336504697769385 2216 7 0.05660472476663614 2216 8 0.01059861843016563 2216 9 -0.0035180707052546722 2216 10-11 0.01378376813234894 2216 12-13 0.013683675310836918 2216 14-15 0.03066865406510999 2216 16-17 0.017774019340521363 2216 18-19 -0.026771079932011332 2216 20-21 -0.03371393862563821 2216 22-23 -0.10196823658451848 2216 24-25 -0.0736641596773282 2216 26-27 -0.04246280513990541 2216 28-29 -0.03351280843333626 2216 30-31 -0.10948129368875925 2216 32-33 -0.016349897031446403 2216 34-35 0.006897044468132663 2216 36-37 -0.0445634781595885 2216 38-39 -0.015099723384118136 2216 40-41 -0.03611280584027554 2216 42-43 -0.0450752885558785 2216 44-45 -0.02349562874466926 2216 46-47 -0.04319947916305722 2216 48-49 -0.073271648802681 2216 50-51 0.036275453141087155 2216 52-53 0.01755189819974845 2216 54-55 -0.03478744436999648 2216 56-57 -0.05173230895530878 2216 58-59 -0.06202582539756918 2216 60-61 -0.08966892354722944 2216 62-63 -0.12056061341769464 2216 64-65 -0.03767225997820134 2216 66-67 -0.033779291715312354 2216 68-69 0.024954545773589132 2216 70-71 -0.03206662702601193 2216 72-73 -0.0010771548258574626 2216 74-75 0.011098351663740402 2216 76-77 0.01495847193011457 2216 78-79 0.07723585074843697 2216 80-81 0.04469709020342805 2216 82-83 0.03373080246969096 2216 84-85 0.03948390640886856 2216 86-87 -0.02286811717538484 2216 88-89 -0.013679485385658552 2216 90-91 -0.01299076359939022 2216 92-93 0.008322558837171812 2216 94-95 -0.028517785237305304 2216 96-97 -6.090711627670942E-4 2216 98-99 -0.02456274900991673 2216 100 -0.0013925376725332228 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52.0 3 296.0 4 85.0 5 146.0 6 333.0 7 829.0 8 1474.0 9 2555.0 10 3824.0 11 5753.0 12 8567.0 13 12889.0 14 14412.0 15 18959.0 16 24579.0 17 36781.0 18 43804.0 19 22355.0 20 13394.0 21 12062.0 22 11873.0 23 11668.0 24 12379.0 25 14610.0 26 17984.0 27 23026.0 28 28895.0 29 36251.0 30 45852.0 31 57817.0 32 73942.0 33 95988.0 34 134687.0 35 223067.0 36 358181.0 37 737819.0 38 1356481.0 39 892243.0 40 8619.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.65776296768479 21.060007226832493 14.719779226453964 22.56245057902876 2 14.276149745608437 22.030756117484106 40.70177930887524 22.99131482803222 3 16.32583950436249 28.378661375467413 32.4311487279126 22.864350392257496 4 11.083825501525823 18.635679297500694 39.81204395157235 30.46845124940114 5 11.1975935489949 39.07397572139778 35.685726447511776 14.042704282095544 6 27.00551330715717 40.19142033817608 18.702536423730294 14.100529930936451 7 23.824805001957827 35.44110466851994 23.68719571472857 17.04689461479366 8 24.678207120077737 35.295567839935146 21.369077227312623 18.657147812674488 9 25.696420920465663 15.84500507500235 22.52547238763395 35.933101616898036 10-11 22.635788358474255 27.613562602717224 29.747950008832564 20.00269902997596 12-13 24.321284463324925 25.99933417817401 29.905847844819984 19.77353351368108 14-15 21.62090515806912 27.003477235409402 28.752118927797614 22.623498678723863 16-17 19.131677607513843 30.322043765985395 29.413882041392302 21.132396585108456 18-19 19.03958675939148 27.765078808645384 33.59199821744737 19.603336214515778 20-21 20.755998754505352 26.82713217067309 33.34975739661375 19.06711167820781 22-23 20.23855265940494 26.92652517602701 33.54348201146103 19.291440153107015 24-25 19.922935573818947 26.982378400423414 33.47327203616426 19.621413989593382 26-27 19.623060756009306 27.055808847839728 33.506559594255776 19.81457080189519 28-29 19.55776849892451 26.989392199470092 33.67793372198206 19.774905579623343 30-31 19.90204202084328 27.298643872889244 33.33800738201836 19.461306724249113 32-33 19.607224281588934 27.213006465496253 33.56324259352584 19.61652665938897 34-35 19.863853680224896 27.02992820132361 33.353213447006645 19.75300467144484 36-37 20.07454032075044 27.171748959527513 32.88405145763525 19.8696592620868 38-39 19.979310285571902 27.291119518614725 32.92195246291804 19.807617732895327 40-41 19.921586004623183 27.166093828724165 32.922800110985676 19.989520055666972 42-43 19.901698002948557 27.29423546907161 32.66046276219584 20.14360376578399 44-45 20.221696646082133 27.477977258680646 32.26155896779087 20.03876712744636 46-47 20.355282810973847 27.788580791073848 31.609387784186264 20.24674861376604 48-49 20.477096864548184 27.768478682375907 31.10538438053647 20.649040072539435 50-51 20.45153694608256 27.89793048048518 30.905503715131726 20.745028858300536 52-53 20.47470707998301 28.10053701430312 30.82020583333448 20.60455007237939 54-55 20.61473627098885 28.30363092865729 30.626754858729754 20.4548779416241 56-57 20.478268475516227 28.069681011146585 30.5814972694611 20.870553243876092 58-59 20.365537192279177 27.803282752603504 30.99499416658189 20.836185888535432 60-61 20.50667239167488 28.02910864234134 30.736523528477754 20.72769543750603 62-63 20.36259081553606 27.899916576526085 30.98747868875071 20.750013919187147 64-65 20.349090110908325 27.77955030180706 31.0937056252428 20.777653962041814 66-67 20.31037365332929 27.844340497514786 30.892022673317427 20.9532631758385 68-69 20.738817739938252 27.968844637552387 30.55514663890947 20.73719098359989 70-71 20.643049317593526 27.791468358186215 30.560583157244757 21.004899166975502 72-73 20.511708104408743 27.91398915734023 30.725764886604356 20.84853785164667 74-75 20.589624846259735 28.03032142942245 30.483624256043758 20.896429468274057 76-77 20.682766120074344 27.997975023920286 30.33098208819529 20.988276767810074 78-79 20.69987164582496 28.042374291039085 30.455499227171313 20.802254835964646 80-81 20.562963828534375 28.014155147565923 30.507974585804988 20.914906438094715 82-83 20.400993111639824 27.96092486958307 30.74457711187445 20.893504906902656 84-85 20.44648084014879 27.991162719829347 30.38300226946487 21.179354170556987 86-87 20.56196245220604 28.01075740000859 30.333748621672324 21.093531526113043 88-89 20.39169282106906 27.858058694923958 30.477726255628983 21.272522228377998 90-91 20.287960994207786 27.905810085050227 30.370708538015982 21.435520382726008 92-93 20.293082214928486 28.00765269591417 30.334576323612804 21.364688765544535 94-95 20.17032677105909 27.894385441791293 30.472531402845643 21.462756384303976 96-97 20.22194417210961 28.012707465492774 30.208446700107526 21.55690166229009 98-99 20.150236641440873 27.92256733251774 30.309029790140464 21.618166235900922 100 20.26742453524682 28.00295840024598 30.076954607389904 21.652662457117287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3752.0 1 2194.0 2 3195.0 3 5442.0 4 3218.5 5 1761.0 6 4131.0 7 10732.5 8 18604.5 9 20333.0 10 13088.0 11 6128.0 12 4995.0 13 5375.5 14 5974.0 15 6420.5 16 7000.5 17 7826.0 18 8653.0 19 9676.0 20 11179.0 21 13448.5 22 17589.0 23 24336.5 24 31678.5 25 38884.5 26 48837.0 27 61497.0 28 77975.5 29 93322.5 30 101672.0 31 110358.5 32 119769.5 33 128680.5 34 137619.0 35 144096.5 36 146690.0 37 148335.5 38 148518.0 39 146005.0 40 145390.0 41 142451.5 42 136937.0 43 133336.0 44 130944.0 45 128067.0 46 125565.5 47 123429.5 48 119457.0 49 115535.0 50 113500.0 51 109432.5 52 102191.5 53 96343.5 54 92984.5 55 90405.0 56 83333.5 57 74052.5 58 67667.5 59 61905.5 60 54561.5 61 45628.0 62 38189.5 63 31135.5 64 24292.0 65 19519.0 66 15617.5 67 11750.5 68 8777.0 69 6570.5 70 4777.5 71 3540.0 72 2688.5 73 2088.5 74 1691.0 75 1276.5 76 799.0 77 602.5 78 447.0 79 254.0 80 143.5 81 58.5 82 28.5 83 19.5 84 12.5 85 6.5 86 4.0 87 2.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0054988726165537604 2 0.004490745970185571 3 0.001168510431017674 4 0.0 5 0.044082628809372644 6 0.0 7 0.0 8 0.0 9 2.2911969235640668E-5 10-11 0.0 12-13 0.0 14-15 1.1455984617820334E-5 16-17 0.0 18-19 8.019189232474233E-5 20-21 0.0 22-23 4.5823938471281337E-5 24-25 2.2911969235640668E-5 26-27 1.8329575388512535E-4 28-29 1.6038378464948465E-4 30-31 1.4892780003166435E-4 32-33 0.001271614292578057 34-35 8.133749078652438E-4 36-37 0.0010883185386929319 38-39 9.96670661750369E-4 40-41 9.164787694256267E-5 42-43 4.926073385662743E-4 44-45 1.9475173850294568E-4 46-47 1.8329575388512535E-4 48-49 1.9475173850294568E-4 50-51 3.5513552315243036E-4 52-53 6.644471078335794E-4 54-55 9.508467232790876E-4 56-57 0.0022797409389462464 58-59 5.269752924197353E-4 60-61 0.0012372463387245961 62-63 0.0011914224002533148 64-65 0.001615293831112667 66-67 4.0095946162371174E-4 68-69 4.0095946162371174E-4 70-71 3.0931158468114903E-4 72-73 2.6348764620986765E-4 74-75 6.186231693622981E-4 76-77 8.019189232474235E-4 78-79 0.00103103861560383 80-81 9.393907386612674E-4 82-83 0.0015351019387879247 84-85 0.001512189969552284 86-87 0.0021422691235324027 88-89 0.0014892780003166433 90-91 0.001168510431017674 92-93 0.0012830702771958772 94-95 8.706548309543454E-4 96-97 2.291196923564067E-4 98-99 3.78047492388071E-4 100 2.291196923564067E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4364531.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.59747780864895 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81195407167283 41.20972954775869 2 16.531268639460777 18.71256219941391 3 5.1667717642982645 8.772787508166749 4 2.1334343042459363 4.82988002763079 5 1.0246207469315514 2.899547499336989 6 0.5935005191280892 2.0154379476464195 7 0.38350609571312677 1.5193834419123704 8 0.24784127148426197 1.1221752690318283 9 0.17356229640769993 0.8840869397417653 >10 0.8419715324719489 8.227919368440917 >50 0.0542469675634341 2.120142967497414 >100 0.03370867713905573 3.598140082796359 >500 0.002232823250473738 0.8070585467626931 >1k 0.0010961128316491176 0.9147273450765117 >5k 1.6238708617020018E-4 0.5334281993241231 >10k+ 1.2179031462765014E-4 1.8329931094624934 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33654 0.771079412656251 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27893 0.6390835578897252 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18229 0.41766228719649373 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6791 0.15559518307923578 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5724 0.13114811190480719 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5651 0.12947553815060542 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5050 0.11570544463998537 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4643 0.10638027316107962 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 2.2911969235640668E-5 9 0.0 0.0 0.0 4.5823938471281337E-5 2.2911969235640668E-5 10-11 0.0 0.0 0.0 4.5823938471281337E-5 2.2911969235640668E-5 12-13 0.0 0.0 0.0 4.5823938471281337E-5 2.2911969235640668E-5 14-15 0.0 0.0 0.0 4.5823938471281337E-5 2.2911969235640668E-5 16-17 0.0 0.0 0.0 5.7279923089101676E-5 6.873590770692201E-5 18-19 0.0 0.0 0.0 1.0310386156038302E-4 6.873590770692201E-5 20-21 0.0 0.0 0.0 2.1766370773858637E-4 6.873590770692201E-5 22-23 2.2911969235640668E-5 1.1455984617820334E-5 0.0 4.696953693306337E-4 6.873590770692201E-5 24-25 2.2911969235640668E-5 3.4367953853461004E-5 0.0 6.873590770692201E-4 6.873590770692201E-5 26-27 2.2911969235640668E-5 4.5823938471281337E-5 0.0 0.0011799664156354945 6.873590770692201E-5 28-29 2.2911969235640668E-5 4.5823938471281337E-5 0.0 0.002543228585156114 6.873590770692201E-5 30-31 2.2911969235640668E-5 4.5823938471281337E-5 0.0 0.006048759878209136 6.873590770692201E-5 32-33 2.2911969235640668E-5 6.873590770692201E-5 0.0 0.013174382310493383 6.873590770692201E-5 34-35 2.2911969235640668E-5 1.03103861560383E-4 0.0 0.023152544912614894 6.873590770692201E-5 36-37 2.2911969235640668E-5 1.1455984617820334E-4 0.0 0.03877850793132183 6.873590770692201E-5 38-39 2.2911969235640668E-5 1.1455984617820334E-4 0.0 0.061392621566899175 6.873590770692201E-5 40-41 2.2911969235640668E-5 1.1455984617820334E-4 0.0 0.08476283018725266 6.873590770692201E-5 42-43 2.2911969235640668E-5 1.2601583079602368E-4 0.0 0.11016074808496032 6.873590770692201E-5 44-45 2.2911969235640668E-5 1.3747181541384402E-4 0.0 0.14183654555323355 6.873590770692201E-5 46-47 2.2911969235640668E-5 1.3747181541384402E-4 0.0 0.18132532453086025 6.873590770692201E-5 48-49 3.4367953853461004E-5 1.3747181541384402E-4 0.0 0.2251330097094052 6.873590770692201E-5 50-51 4.5823938471281337E-5 1.3747181541384402E-4 0.0 0.270544532734445 6.873590770692201E-5 52-53 4.5823938471281337E-5 1.3747181541384402E-4 0.0 0.3143407619283721 6.873590770692201E-5 54-55 4.5823938471281337E-5 1.3747181541384402E-4 0.0 0.3632921842003184 6.873590770692201E-5 56-57 5.7279923089101676E-5 1.3747181541384402E-4 0.0 0.4257158443828214 6.873590770692201E-5 58-59 6.873590770692201E-5 1.3747181541384402E-4 0.0 0.49481834359751364 6.873590770692201E-5 60-61 6.873590770692201E-5 1.3747181541384402E-4 0.0 0.5703018262443318 6.873590770692201E-5 62-63 9.164787694256267E-5 1.3747181541384402E-4 0.0 0.6480306818762429 8.019189232474234E-5 64-65 9.164787694256267E-5 1.3747181541384402E-4 0.0 0.7318426653402164 9.164787694256267E-5 66-67 9.164787694256267E-5 1.3747181541384402E-4 0.0 0.8343049917620015 9.164787694256267E-5 68-69 9.164787694256267E-5 1.3747181541384402E-4 0.0 0.951660098186953 9.164787694256267E-5 70-71 9.164787694256267E-5 1.8329575388512535E-4 0.0 1.0857180301846865 9.164787694256267E-5 72-73 9.164787694256267E-5 1.8329575388512535E-4 0.0 1.2246103876911403 9.164787694256267E-5 74-75 1.1455984617820334E-4 1.8329575388512535E-4 0.0 1.3664469332443738 9.164787694256267E-5 76-77 1.1455984617820334E-4 1.8329575388512535E-4 0.0 1.523611586216251 9.164787694256267E-5 78-79 1.1455984617820334E-4 1.8329575388512535E-4 0.0 1.7101150157943659 9.164787694256267E-5 80-81 1.1455984617820334E-4 1.8329575388512535E-4 0.0 1.9184191841001932 9.164787694256267E-5 82-83 1.4892780003166435E-4 1.8329575388512535E-4 0.0 2.1426586270094083 9.164787694256267E-5 84-85 1.6038378464948468E-4 1.8329575388512535E-4 0.0 2.3691663548729522 9.164787694256267E-5 86-87 1.6038378464948468E-4 1.8329575388512535E-4 0.0 2.61183847703224 9.164787694256267E-5 88 1.8329575388512535E-4 1.8329575388512535E-4 0.0 2.810702914012983 9.164787694256267E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12135 0.0 62.2712 1 GTATCAA 24390 0.0 45.499992 1 GTGGTAT 4220 0.0 38.88876 1 TGGTATC 4175 0.0 37.728504 2 ATCAACG 31600 0.0 34.80202 3 TATCAAC 31830 0.0 34.566906 2 TCAACGC 31840 0.0 34.495 4 CAACGCA 32845 0.0 33.453827 5 AACGCAG 34330 0.0 32.185184 6 CGCAGAG 38220 0.0 28.74955 8 ACGCAGA 38425 0.0 28.63286 7 GCAGAGT 41375 0.0 26.375544 9 GTACATG 21385 0.0 25.858847 1 TATAACG 450 0.0 25.077253 2 CATGGGT 9895 0.0 25.040972 4 TACATGG 21760 0.0 24.871256 2 ACATGGG 21645 0.0 24.307137 3 ATAACGC 560 0.0 23.508848 3 GAGTACT 28825 0.0 23.095346 12-13 CAGAGTA 40775 0.0 22.308811 10-11 >>END_MODULE