FastQCFastQC Report
Wed 25 May 2016
SRR2431385_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431385_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850968
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310320.8058233670079835No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38710.10052018089997113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.048.0486071
GTATCAA118700.038.7624971
ATCAACG142100.031.1539823
TCAACGC142000.031.1411694
CAACGCA149400.029.6616765
GTACATG273750.029.198661
TACATGG265500.028.6352162
TATCAAC161500.028.1111352
AACGCAG159300.027.9368826
CATGGGT120750.027.8557134
ACATGGG274000.026.5446433
CGCAGAG175100.024.8795728
ATGGGTA55650.024.3457245
ACGCAGA180750.024.2838697
ATGGGTG62000.023.369775
TACCTGG25350.022.6415162
GCAGAGT187450.022.4133669
CATGGGG94350.021.0908374
GTACGGA4250.021.0091446
GTACCTG30400.020.2731551
Produced by FastQC (version 0.11.5)