FastQCFastQC Report
Wed 25 May 2016
SRR2431385_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431385_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850968
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT427961.111305001755403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337550.8765328613481078No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237520.6167799888235893No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99440.2582207902013208No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68310.17738397202988962No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64370.1671527782105694No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62890.16330958865407347No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61460.15959623658259428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131850.066.116491
GTATCAA272200.046.3115041
ATCAACG360300.034.7368283
GTGGTAT40950.034.331871
TCAACGC365350.034.2305034
TATCAAC368500.033.9779322
CAACGCA376700.033.2116135
TATAACG3400.033.189112
TGGTATC41300.033.014992
AACGCAG390250.032.1788646
ACGCAGA436500.028.7154717
CGCAGAG434650.028.6646868
GCAGAGT460400.026.8879439
CATGGGT109900.026.6092994
GTACATG255250.026.3236871
TACATGG248700.026.2407932
ACATGGG248400.025.3250833
TACCTGG23050.024.681852
GAGTACT326900.022.70096812-13
ATGGGTG60900.022.697125
Produced by FastQC (version 0.11.5)