##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431385_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3850968
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.611496122533346	34.0	31.0	34.0	31.0	34.0
2	32.700819378400446	34.0	31.0	34.0	31.0	34.0
3	32.79926164019021	34.0	31.0	34.0	31.0	34.0
4	36.2835146903324	37.0	35.0	37.0	35.0	37.0
5	36.2058882338155	37.0	37.0	37.0	35.0	37.0
6	36.26144127917968	37.0	37.0	37.0	35.0	37.0
7	36.21265588288451	37.0	37.0	37.0	35.0	37.0
8	36.22473726086532	37.0	37.0	37.0	35.0	37.0
9	38.00744721846559	39.0	39.0	39.0	35.0	39.0
10-11	37.981475177150266	39.0	38.5	39.0	35.0	39.0
12-13	37.96517018578186	39.0	38.0	39.0	35.0	39.0
14-15	39.37444117946448	41.0	39.0	41.0	36.0	41.0
16-17	39.41354719125165	41.0	39.0	41.0	36.0	41.0
18-19	39.515022197016435	41.0	39.5	41.0	36.5	41.0
20-21	39.50950280552837	41.0	40.0	41.0	36.0	41.0
22-23	39.437058033201	41.0	39.0	41.0	36.0	41.0
24-25	39.388389230967384	41.0	39.0	41.0	36.0	41.0
26-27	39.2600831790864	41.0	39.0	41.0	35.5	41.0
28-29	39.172975729738596	41.0	39.0	41.0	35.0	41.0
30-31	38.94309015291739	41.0	38.5	41.0	35.0	41.0
32-33	38.66851542261582	41.0	38.0	41.0	35.0	41.0
34-35	38.51535367211568	40.5	38.0	41.0	35.0	41.0
36-37	38.361802798672954	40.0	38.0	41.0	35.0	41.0
38-39	38.20821024739754	40.0	38.0	41.0	34.5	41.0
40-41	38.05280373661895	40.0	38.0	41.0	34.0	41.0
42-43	37.84256322046821	40.0	37.5	41.0	33.5	41.0
44-45	37.54616761292226	40.0	37.0	41.0	33.0	41.0
46-47	37.07937445857769	40.0	37.0	41.0	32.0	41.0
48-49	36.65929215719268	40.0	37.0	41.0	30.5	41.0
50-51	36.472179592247976	40.0	36.0	41.0	30.0	41.0
52-53	36.40923723593652	40.0	36.0	41.0	30.0	41.0
54-55	36.261010348566906	40.0	35.5	41.0	30.0	41.0
56-57	36.10408603758847	40.0	35.0	41.0	30.0	41.0
58-59	35.917132783237875	39.5	35.0	41.0	29.5	41.0
60-61	35.754529380664806	39.0	35.0	41.0	29.0	41.0
62-63	35.58588996325079	39.0	35.0	41.0	29.0	41.0
64-65	35.349118195736764	39.0	35.0	41.0	29.0	41.0
66-67	35.08106195637045	38.0	35.0	41.0	28.0	41.0
68-69	34.785057938679316	37.0	35.0	40.0	28.0	41.0
70-71	34.48948407257603	37.0	35.0	40.0	28.0	41.0
72-73	34.16820472151417	36.0	35.0	39.0	27.0	41.0
74-75	33.84111812925997	36.0	35.0	39.0	26.5	41.0
76-77	33.52462406854588	35.5	34.0	39.0	26.0	40.5
78-79	33.21120754573915	35.0	34.0	37.0	26.0	39.5
80-81	32.95542873895602	35.0	34.0	37.0	26.0	39.0
82-83	32.76664425671676	35.0	34.0	37.0	26.0	39.0
84-85	32.51149866734805	35.0	34.0	36.0	26.0	38.0
86-87	32.27643789821157	35.0	34.0	36.0	26.0	37.0
88-89	32.050257493700286	35.0	34.0	36.0	25.0	37.0
90-91	31.870838968280182	35.0	34.0	35.0	25.0	36.5
92-93	31.712339728608494	35.0	34.0	35.0	25.0	36.0
94-95	31.569018101422813	35.0	34.0	35.0	24.0	36.0
96-97	31.423353297145027	35.0	34.0	35.0	23.5	36.0
98-99	31.305683921549075	35.0	34.0	35.0	22.0	36.0
100	31.200113322156923	35.0	34.0	35.0	20.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.005933152807770625
1101	2	0.009553462416150182
1101	3	0.02059181266737653
1101	4	0.069690414057618
1101	5	0.02694067149436563
1101	6	0.05397268967254121
1101	7	0.08255315281989084
1101	8	0.09078392720063988
1101	9	0.07309283771336084
1101	10-11	0.07561794793296883
1101	12-13	0.06469229654281605
1101	14-15	0.08207904097354657
1101	16-17	0.05672003201398468
1101	18-19	0.037139214606298765
1101	20-21	0.06710958095232655
1101	22-23	0.0693711489607125
1101	24-25	0.10856777521028249
1101	26-27	0.15926442833722376
1101	28-29	0.09053682052155665
1101	30-31	0.13013121048417275
1101	32-33	0.10059681405250842
1101	34-35	0.13879090223312573
1101	36-37	0.20301310627390734
1101	38-39	0.16230275405211358
1101	40-41	0.1181018446861728
1101	42-43	0.13551277820177177
1101	44-45	0.126727073976582
1101	46-47	0.18292932762867053
1101	48-49	0.19772603859890836
1101	50-51	0.16253092922077172
1101	52-53	0.2551022787439763
1101	54-55	0.2388620219637474
1101	56-57	0.23434316374675035
1101	58-59	0.22453446891038453
1101	60-61	0.20545185491697993
1101	62-63	0.20822664219893738
1101	64-65	0.22583092483428402
1101	66-67	0.1962711987456629
1101	68-69	0.18014859601480282
1101	70-71	0.2296064897055743
1101	72-73	0.15832942072000833
1101	74-75	0.16259833053624817
1101	76-77	0.12749996340625103
1101	78-79	0.12693966392540545
1101	80-81	0.1848839136724365
1101	82-83	0.201706023164121
1101	84-85	0.184009854208945
1101	86-87	0.2518354786907224
1101	88-89	0.2556569195954381
1101	90-91	0.1303258963724403
1101	92-93	0.10117969606720223
1101	94-95	0.08622723088829787
1101	96-97	0.17387791660952345
1101	98-99	0.1697545810157699
1101	100	0.15561107760871096
1102	1	-0.008917531600289408
1102	2	0.0011999265152127236
1102	3	0.02304847069449778
1102	4	0.04578541718478135
1102	5	0.02924007299329645
1102	6	-0.04052272355199449
1102	7	-0.046588603951882135
1102	8	0.002057374667423062
1102	9	0.01854949961141017
1102	10-11	0.047450435099456456
1102	12-13	0.04475942676255329
1102	14-15	0.0352151785375483
1102	16-17	-0.00863712901282554
1102	18-19	0.019013343301445218
1102	20-21	0.02995134950279521
1102	22-23	0.09377732757315727
1102	24-25	0.06103113132864024
1102	26-27	0.1096249708978263
1102	28-29	0.06071158194785653
1102	30-31	0.0760466812639109
1102	32-33	0.11096717356788588
1102	34-35	0.10512400764241647
1102	36-37	0.10945224613116267
1102	38-39	0.09397603678318234
1102	40-41	0.12243490143234226
1102	42-43	0.1563118722382768
1102	44-45	0.12202914752314342
1102	46-47	0.02774976071937374
1102	48-49	0.010430402961794982
1102	50-51	0.039577994475259004
1102	52-53	0.0437375810166003
1102	54-55	0.060972089367702154
1102	56-57	0.1181141034039257
1102	58-59	0.14655451645646878
1102	60-61	0.13401888919295857
1102	62-63	0.13899091307334288
1102	64-65	0.1056720411330545
1102	66-67	0.1162142504540995
1102	68-69	0.0718793427005977
1102	70-71	0.050644205834800005
1102	72-73	0.05027272293290963
1102	74-75	0.07794489489238998
1102	76-77	0.06256055175442299
1102	78-79	0.036477483187262294
1102	80-81	0.0019338116181302212
1102	82-83	0.05407680875828902
1102	84-85	0.025177926668334294
1102	86-87	0.05371091658967231
1102	88-89	0.07693473706561349
1102	90-91	0.07869287546001758
1102	92-93	0.03254627672549404
1102	94-95	0.08344721196498028
1102	96-97	0.06388654878325539
1102	98-99	0.04521618940927041
1102	100	0.10026389943367775
1103	1	-0.038632521346912085
1103	2	-0.049500337025200736
1103	3	-0.016067227742624368
1103	4	0.020671962727874416
1103	5	-0.008388008266024372
1103	6	-0.0115120216512139
1103	7	-0.05326564896975583
1103	8	0.008662463728391856
1103	9	0.008712308562841997
1103	10-11	0.007386479599325924
1103	12-13	0.028927052498019634
1103	14-15	-0.00962139682668095
1103	16-17	0.04222768390512499
1103	18-19	-0.04780312842144241
1103	20-21	-0.058049444351652824
1103	22-23	-0.06332993977211743
1103	24-25	-0.06501799133837238
1103	26-27	-0.09748559844165072
1103	28-29	-0.0453356076767264
1103	30-31	-0.06842873848490427
1103	32-33	-0.10810906603856552
1103	34-35	-0.10558852143060449
1103	36-37	-0.11571706706092755
1103	38-39	-0.10339258340287216
1103	40-41	-0.1297989320384545
1103	42-43	-0.1251920760524996
1103	44-45	-0.1434316492067822
1103	46-47	-0.13623315283648196
1103	48-49	-0.16752912972346934
1103	50-51	-0.2333567629347968
1103	52-53	-0.18431138067820285
1103	54-55	-0.17140613067856947
1103	56-57	-0.1480599928643329
1103	58-59	-0.17597069048570546
1103	60-61	-0.25822837238757756
1103	62-63	-0.19826332053036566
1103	64-65	-0.17536907763760468
1103	66-67	-0.14886970618796624
1103	68-69	-0.14778461747735605
1103	70-71	-0.18894570712325276
1103	72-73	-0.2705224454240778
1103	74-75	-0.23133666684079657
1103	76-77	-0.18043729438227984
1103	78-79	-0.17504300259111716
1103	80-81	-0.21279593735464175
1103	82-83	-0.21873123462440702
1103	84-85	-0.2415052614345825
1103	86-87	-0.20837235282485267
1103	88-89	-0.22455050680385824
1103	90-91	-0.22902027342483677
1103	92-93	-0.2861079955390622
1103	94-95	-0.28670372356564755
1103	96-97	-0.20225084719533015
1103	98-99	-0.19638216151978938
1103	100	-0.17567673896562397
1104	1	-0.14684896609654174
1104	2	-0.07967413515898158
1104	3	-0.10694395445347027
1104	4	-0.032214868008466624
1104	5	-0.08298299479871218
1104	6	-0.11308032220857456
1104	7	-0.06646624763697417
1104	8	-0.09978693831195073
1104	9	-0.10044304099776014
1104	10-11	-0.11895066627290163
1104	12-13	-0.05623073108553456
1104	14-15	-0.10566553674122048
1104	16-17	-0.10116554759727592
1104	18-19	-0.08918852684444545
1104	20-21	-0.059445099095057685
1104	22-23	-0.04038455636295879
1104	24-25	-0.036143922375735826
1104	26-27	-0.061155379965846635
1104	28-29	-0.09844158134318803
1104	30-31	-0.06940713215105632
1104	32-33	-0.010294396804894745
1104	34-35	-0.0303771448666339
1104	36-37	-0.02368761619187154
1104	38-39	-0.1167502277646264
1104	40-41	-0.16073804574951112
1104	42-43	-0.2039327522014247
1104	44-45	-0.1904227803872729
1104	46-47	-0.15477812329734064
1104	48-49	-0.15151333283366597
1104	50-51	-0.25020320721946376
1104	52-53	-0.12624776992780085
1104	54-55	-0.012997059832464686
1104	56-57	-0.14125423018695926
1104	58-59	-0.10724542577222707
1104	60-61	-0.15530575908792343
1104	62-63	-0.12459460372274123
1104	64-65	-0.08602011812850918
1104	66-67	-0.1492983219957722
1104	68-69	-0.2584300648368867
1104	70-71	-0.1824456798314742
1104	72-73	-0.1711809187148532
1104	74-75	-0.1297346727209998
1104	76-77	-0.15794647082400814
1104	78-79	-0.15582257728402027
1104	80-81	-0.15343667435414687
1104	82-83	-0.19837311248794265
1104	84-85	-0.17622705032016484
1104	86-87	-0.17294427948339575
1104	88-89	-0.1404566985350506
1104	90-91	-0.13329901891916407
1104	92-93	-0.1505105783527867
1104	94-95	-0.13814641452222887
1104	96-97	-0.13331223484448174
1104	98-99	-0.17380228587598978
1104	100	-0.1271733733156708
1105	1	-0.1128570484702891
1105	2	-0.1471070043357514
1105	3	-0.11131391495779042
1105	4	2.711701526081356E-4
1105	5	-0.0702655587953771
1105	6	-0.015774235869109532
1105	7	0.01909531031500933
1105	8	-0.015489359418637605
1105	9	-0.021464436792108188
1105	10-11	-0.07602342879466306
1105	12-13	-0.09262051120860804
1105	14-15	-0.10973219895657138
1105	16-17	-0.14046457558607983
1105	18-19	-0.1979351017620914
1105	20-21	-0.13781384403336006
1105	22-23	-0.15234904933805637
1105	24-25	-0.17773844680514372
1105	26-27	-0.09860793996752903
1105	28-29	-0.11891386980605745
1105	30-31	-0.11051083933740102
1105	32-33	-0.08962838698242592
1105	34-35	-0.04881339979222332
1105	36-37	-0.09139077776426774
1105	38-39	-0.06062974541125499
1105	40-41	-0.0646412307473625
1105	42-43	-0.0844623928119006
1105	44-45	-0.0452624660011125
1105	46-47	-0.10394566951696049
1105	48-49	-0.1633401040636997
1105	50-51	-0.18973604809981026
1105	52-53	-0.19199659702391614
1105	54-55	-0.1290256444191371
1105	56-57	-0.09801263752787293
1105	58-59	-0.07796151952491925
1105	60-61	-0.1446481017503345
1105	62-63	-0.18049686488514283
1105	64-65	-0.18788495604568567
1105	66-67	-0.21618174109389798
1105	68-69	-0.14384655504917987
1105	70-71	-0.15163981136716131
1105	72-73	-0.08630239984665877
1105	74-75	-0.16104383574361947
1105	76-77	-0.1809034620522567
1105	78-79	-0.08501780099674505
1105	80-81	-0.07736422010710697
1105	82-83	-0.14655231099082044
1105	84-85	-0.15263399943992795
1105	86-87	-0.11683121233322424
1105	88-89	-0.10608431507188953
1105	90-91	-0.11002032321979272
1105	92-93	-0.12364598925687886
1105	94-95	-0.09709126867770479
1105	96-97	-0.11310887108420786
1105	98-99	-0.1336337841168067
1105	100	-0.14332124710008287
1106	1	-0.12293972309871748
1106	2	-0.09143128698827496
1106	3	-0.0936610953619379
1106	4	-0.04220215302969166
1106	5	-0.07004060620842267
1106	6	-0.07917722314105902
1106	7	-0.06752379990724933
1106	8	-0.05898572006533698
1106	9	-0.11162403427873357
1106	10-11	-0.07374839168515734
1106	12-13	-0.09310428227403378
1106	14-15	-0.11750994822877914
1106	16-17	-0.12912410257733598
1106	18-19	-0.09720750905733411
1106	20-21	-0.06998544736191548
1106	22-23	-0.09688916315108997
1106	24-25	-0.03925818060390185
1106	26-27	-0.1259507655694705
1106	28-29	-0.10875229723507829
1106	30-31	-0.1668943857665326
1106	32-33	-0.09273833013916288
1106	34-35	-0.14823648474555284
1106	36-37	-0.09295054168577366
1106	38-39	-0.09425071624476544
1106	40-41	-0.2181078727962955
1106	42-43	-0.2241433646121891
1106	44-45	-0.1929790470834547
1106	46-47	-0.22280272826620262
1106	48-49	-0.1314866292523007
1106	50-51	-0.1610068934745712
1106	52-53	-0.16718401677812267
1106	54-55	-0.18798659286090214
1106	56-57	-0.16901225545032617
1106	58-59	-0.13904261654594308
1106	60-61	-0.17986872477474947
1106	62-63	-0.2303663550044348
1106	64-65	-0.17151301794959295
1106	66-67	-0.15887704241550438
1106	68-69	-0.16455513237534802
1106	70-71	-0.11030495722100397
1106	72-73	-0.10979938605387929
1106	74-75	-0.11571798212906259
1106	76-77	-0.10357956460827467
1106	78-79	-0.11555841340516082
1106	80-81	-0.10641652243022293
1106	82-83	-0.12761303480268538
1106	84-85	-0.10230815045797215
1106	86-87	-0.12370111609563139
1106	88-89	-0.09542847620401318
1106	90-91	-0.09324081231419257
1106	92-93	-0.0861343386713429
1106	94-95	-0.026408376713316528
1106	96-97	-0.012449297703064133
1106	98-99	-0.014746885044289115
1106	100	0.03456190400866532
1107	1	-0.23548817182961557
1107	2	-0.14378102707053841
1107	3	-0.14676007570479044
1107	4	-0.16035100836728589
1107	5	-0.21633879417417035
1107	6	-0.1677535974824167
1107	7	-0.13468509371111992
1107	8	-0.16920990082661547
1107	9	-0.1106980843521228
1107	10-11	-0.07575374419352698
1107	12-13	-0.10781578255903668
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2216	32-33	0.013360595330695446
2216	34-35	0.06611414180440534
2216	36-37	0.12896820342141524
2216	38-39	0.0728276521304636
2216	40-41	0.09286517905267999
2216	42-43	0.16565748812271863
2216	44-45	0.130987679786557
2216	46-47	0.15968843588530035
2216	48-49	0.21530831804911799
2216	50-51	0.21785966521205324
2216	52-53	0.1787404929888936
2216	54-55	0.2528252738675789
2216	56-57	0.16464702581707513
2216	58-59	0.18677519155203726
2216	60-61	0.19943835421252487
2216	62-63	0.1271360221033433
2216	64-65	0.1793886274175307
2216	66-67	0.21599562948090067
2216	68-69	0.22410319425082292
2216	70-71	0.16582816977413017
2216	72-73	0.15797952244717806
2216	74-75	0.20268173289556302
2216	76-77	0.20513515572567798
2216	78-79	0.17833681056934125
2216	80-81	0.1918388871668668
2216	82-83	0.23275469026999218
2216	84-85	0.24352552109412073
2216	86-87	0.2123328260866657
2216	88-89	0.23409366122234587
2216	90-91	0.22421885744179093
2216	92-93	0.22146979866240457
2216	94-95	0.15196470477068047
2216	96-97	0.19098216638545296
2216	98-99	0.2216242297960065
2216	100	0.24710416593426743
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42.0
3	345.0
4	98.0
5	144.0
6	397.0
7	914.0
8	1618.0
9	2739.0
10	3865.0
11	5660.0
12	8707.0
13	13222.0
14	14259.0
15	18715.0
16	25318.0
17	40233.0
18	47118.0
19	23498.0
20	13969.0
21	11527.0
22	11558.0
23	11381.0
24	12357.0
25	14652.0
26	17945.0
27	22808.0
28	28249.0
29	34975.0
30	44094.0
31	54613.0
32	68513.0
33	88461.0
34	123859.0
35	212643.0
36	340705.0
37	681001.0
38	1147738.0
39	695762.0
40	7266.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40519790120716	21.18573225978599	14.918101114448094	21.49096872455876
2	14.676873760318024	22.14329749822504	40.904116717989524	22.275712023467417
3	16.091168721497116	29.99761615777608	32.17865001820341	21.732565102523395
4	10.631301013147864	19.057208473298143	40.2309497248484	30.080540788705594
5	10.63620079831971	39.983503644135936	35.59918791167825	13.781107645866102
6	25.89522945919052	40.97551576642548	18.90142945877504	14.227825315608959
7	23.50175332539767	36.087134455544685	24.099784781384837	16.311327437672812
8	25.261207052356703	34.6954843561411	21.069014336135748	18.97429425536644
9	26.672275650388926	15.459138164070566	22.568161486796168	35.300424698744344
10-11	22.860875499354968	27.880275816366172	29.355502304875035	19.90334637940383
12-13	24.698452441048588	26.71762008928664	29.533963928030566	19.049963541634206
14-15	21.892807211070046	26.98874672549863	29.26945900355443	21.848987059876894
16-17	18.878214516454044	30.0952903270035	29.85732418446479	21.16917097207767
18-19	18.91333905657859	27.284701848525124	34.70025152156786	19.10170757332843
20-21	20.624476754935383	26.111954189180487	34.746575406495204	18.516993649388933
22-23	19.97225631899728	26.254769256656836	34.996015286550104	18.776959137795778
24-25	19.40729936332858	26.668983834186022	34.84751618165416	19.07620062083124
26-27	18.964077162527552	26.85640278242128	34.879384050091375	19.3001360049598
28-29	19.15714135102725	26.6072590617523	35.243975148053764	18.991624439166692
30-31	19.410980562117054	26.738712780542095	34.86936177853857	18.980944878802283
32-33	19.0321730824088	26.72519489247399	35.012972922861906	19.2296591022553
34-35	19.648259128992557	26.58450299622312	34.65586183498719	19.111376039797122
36-37	19.687126306345377	26.643161430465227	34.10428430645704	19.56542795673236
38-39	19.461051215650276	26.867938786096058	34.29959964138463	19.37141035686904
40-41	19.359313085657462	26.71971057610173	34.22885017906572	19.692126159175093
42-43	19.364527160681533	26.940216079514016	34.01047042107992	19.68478633872452
44-45	19.692326229134434	26.96720975174515	33.48638599913476	19.854078019985653
46-47	19.956667958984784	27.168634011094895	32.79177587100986	20.082922158910463
48-49	20.16965626519912	27.300262298027793	32.14846520208062	20.381616234692466
50-51	20.229615740820687	27.573676195427343	31.64922316032995	20.54748490342202
52-53	20.220434720286185	27.721364364403872	31.59509791905137	20.463102996258574
54-55	20.240536514138263	28.013055537450278	31.604990271188825	20.141417677222638
56-57	20.366163534261695	27.64827474275327	31.389541338007596	20.59602038497744
58-59	20.419912296897362	27.257957588094566	31.673416954323187	20.648713160684892
60-61	20.460519840899902	27.475603811983092	31.60690112386452	20.456975223252485
62-63	20.18964049611028	27.470294096109033	31.807688452467698	20.53237695531299
64-65	20.25751682596301	27.46437580468328	31.7847362196934	20.493371149660312
66-67	20.274885667718312	27.429191758441725	31.66402078034582	20.631901793494144
68-69	20.654663413448503	27.660170617373968	31.38854352010254	20.296622449074984
70-71	20.548567894011732	27.450064652802315	31.43777730949561	20.563590143690348
72-73	20.38283166424801	27.46889315824113	31.486526157168797	20.661749020342064
74-75	20.3151724403876	27.640852316687912	31.435822349325406	20.608152893599083
76-77	20.360663247062465	27.689328793827368	31.188091301510568	20.7619166575996
78-79	20.370655293848653	27.627993865907683	31.42399257946736	20.577358260776307
80-81	20.25514319832155	27.77610790936384	31.299654330746385	20.669094561568222
82-83	20.137484547465526	27.62813836901477	31.530610138316817	20.703766945202887
84-85	20.151166631523513	27.678310822671804	31.15382158426722	21.016700961537463
86-87	20.318596190728204	27.601763071349545	31.039108196578276	21.040532541343975
88-89	20.108815753562855	27.585523225271512	31.296980741969506	21.008680279196128
90-91	20.01457051769613	27.593641738729403	31.16472994684137	21.2270577967331
92-93	20.016453202635628	27.68224077246955	31.22601548408407	21.075290540810744
94-95	20.023625440428923	27.42303911506132	31.323989365176004	21.22934607933375
96-97	20.1441951013292	27.727999464030166	31.001609207878655	21.12619622676198
98-99	19.834900672028194	27.610985795859182	31.076866787182244	21.47724674493038
100	19.859110382403546	27.587343201354052	31.04759906495994	21.505947351282458
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5003.0
1	2892.5
2	4365.0
3	7180.5
4	4034.5
5	2367.0
6	5161.5
7	13455.0
8	23650.5
9	25472.0
10	16447.0
11	7939.5
12	6375.0
13	6579.0
14	6877.0
15	7360.5
16	7864.0
17	8385.0
18	9075.0
19	9914.0
20	11258.5
21	13409.0
22	16745.0
23	21961.5
24	27552.0
25	32925.0
26	40608.0
27	50093.5
28	62855.5
29	74631.5
30	80435.0
31	87184.0
32	96756.5
33	106099.5
34	113280.0
35	120044.0
36	123590.5
37	126165.5
38	130355.0
39	131274.5
40	134147.5
41	135213.5
42	133048.0
43	133965.5
44	133567.5
45	129555.0
46	125058.0
47	122398.0
48	117445.5
49	112699.5
50	112614.5
51	104147.0
52	87718.5
53	75806.0
54	69571.5
55	66666.0
56	60496.0
57	52790.0
58	48915.0
59	45023.5
60	39317.0
61	32870.5
62	27276.0
63	22065.0
64	17275.5
65	14459.0
66	12041.0
67	9241.0
68	7045.0
69	5441.5
70	4210.5
71	3335.0
72	2580.5
73	1981.5
74	1729.5
75	1377.0
76	877.0
77	686.5
78	550.0
79	332.0
80	163.0
81	70.5
82	34.5
83	16.0
84	6.0
85	2.5
86	2.5
87	3.5
88	3.0
89	2.5
90	1.5
91	0.5
92	0.0
93	1.0
94	1.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004752051951613205
2	0.004726084454609854
3	0.0010906348741407355
4	0.0
5	0.04240492260647193
6	0.0
7	0.0
8	0.0
9	7.790249101005254E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	1.5580498202010507E-4
20-21	0.0
22-23	2.5967497003350842E-5
24-25	5.1934994006701684E-5
26-27	1.4282123351842964E-4
28-29	5.1934994006701684E-5
30-31	2.2072372452848217E-4
32-33	0.001622968562709428
34-35	9.478136406223059E-4
36-37	0.0013762773411775948
38-39	0.0011815211136524636
40-41	1.9475622752513133E-4
42-43	6.362036765820958E-4
44-45	1.5580498202010507E-4
46-47	1.8177247902345593E-4
48-49	1.2983748501675421E-4
50-51	3.2459371254188555E-4
52-53	8.30959904107227E-4
54-55	9.088623951172796E-4
56-57	0.0024149772213116283
58-59	5.453174370703678E-4
60-61	0.0015320823231976999
62-63	0.0012983748501675422
64-65	0.0019215947782479626
66-67	5.583011855720432E-4
68-69	3.1160996404021015E-4
70-71	3.505612095452364E-4
72-73	1.687887305217805E-4
74-75	7.40073664595499E-4
76-77	9.088623951172796E-4
78-79	8.179761556055517E-4
80-81	8.439436526089026E-4
82-83	0.001622968562709428
84-85	0.001350309844174244
86-87	0.0019345785267496378
88-89	0.0014282123351842964
90-91	0.0010646673771373847
92-93	0.001194504862154139
94-95	8.958786466156043E-4
96-97	1.2983748501675421E-4
98-99	3.505612095452364E-4
100	2.5967497003350843E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3850968.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.067607927807
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.71430626358035	30.280751074547123
2	17.019572821252897	15.000237243714654
3	6.25354490170771	8.267362946621752
4	2.8475841959753554	5.01944895558646
5	1.490561927993227	3.284274931746081
6	0.8743801104867123	2.3119103933280605
7	0.5652069398745758	1.7435122477127876
8	0.37485194788965714	1.3215062936460922
9	0.2621022014956966	1.0395195347274686
>10	1.3263689856133944	10.84575628054663
>50	0.1446491878671064	4.463558577695043
>100	0.11711290193574832	9.613442681519233
>500	0.007628680301714896	2.214081916843439
>1k	0.001655837575760325	1.0517410264344145
>5k	2.956852813857723E-4	0.9289710186160755
>10k+	1.7741116883146336E-4	2.6139248767146803
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42796	1.111305001755403	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33755	0.8765328613481078	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23752	0.6167799888235893	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9944	0.2582207902013208	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6831	0.17738397202988962	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6437	0.1671527782105694	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6289	0.16330958865407347	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6146	0.15959623658259428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	2.5967497003350846E-5	0.0	0.0	2.5967497003350846E-5	0.0
10-11	2.5967497003350846E-5	0.0	0.0	2.5967497003350846E-5	0.0
12-13	3.895124550502627E-5	0.0	0.0	2.5967497003350846E-5	0.0
14-15	5.193499400670169E-5	0.0	0.0	2.5967497003350846E-5	0.0
16-17	5.193499400670169E-5	0.0	0.0	6.491874250837712E-5	0.0
18-19	5.193499400670169E-5	0.0	0.0	9.088623951172795E-5	0.0
20-21	5.193499400670169E-5	0.0	0.0	1.5580498202010507E-4	0.0
22-23	6.491874250837712E-5	0.0	0.0	5.19349940067017E-4	0.0
24-25	7.790249101005254E-5	0.0	0.0	0.0010257161316323585	0.0
26-27	7.790249101005254E-5	0.0	0.0	0.0016619198082144541	0.0
28-29	7.790249101005254E-5	0.0	0.0	0.0032329533769171804	0.0
30-31	7.790249101005254E-5	0.0	0.0	0.006946305448396351	0.0
32-33	7.790249101005254E-5	0.0	0.0	0.012503349807113432	0.0
34-35	7.790249101005254E-5	0.0	0.0	0.021669876249296283	0.0
36-37	7.790249101005254E-5	0.0	0.0	0.03479644598449013	0.0
38-39	7.790249101005254E-5	0.0	0.0	0.05602487478472945	0.0
40-41	7.790249101005254E-5	0.0	0.0	0.07952545957276197	0.0
42-43	7.790249101005254E-5	0.0	0.0	0.10223403570219228	0.0
44-45	7.790249101005254E-5	0.0	0.0	0.131006022381905	0.0
46-47	7.790249101005254E-5	0.0	0.0	0.1672436644500811	0.0
48-49	7.790249101005254E-5	0.0	0.0	0.2076750572842984	0.0
50-51	7.790249101005254E-5	0.0	0.0	0.2504565085973189	0.0
52-53	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.2947181072395304	0.0
54-55	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.34113500813302006	0.0
56-57	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.3999384050971081	0.0
58-59	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.4666359211502147	0.0
60-61	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.5410457838133166	0.0
62-63	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.619831169721483	0.0
64-65	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.6997721092462986	0.0
66-67	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.7919307561111908	0.0
68-69	7.790249101005254E-5	2.5967497003350846E-5	0.0	0.9043700181357	0.0
70-71	7.790249101005254E-5	2.5967497003350846E-5	0.0	1.0308447122905202	0.0
72-73	7.790249101005254E-5	2.5967497003350846E-5	0.0	1.1636035407201515	0.0
74-75	7.790249101005254E-5	2.5967497003350846E-5	0.0	1.2963234179042775	0.0
76-77	7.790249101005254E-5	2.5967497003350846E-5	0.0	1.442273734811611	0.0
78-79	7.790249101005254E-5	2.5967497003350846E-5	0.0	1.614529126183339	0.0
80-81	7.790249101005254E-5	2.5967497003350846E-5	0.0	1.812206697121347	0.0
82-83	7.790249101005254E-5	2.5967497003350846E-5	0.0	2.0219721379144153	0.0
84-85	7.790249101005254E-5	2.5967497003350846E-5	0.0	2.234879645844889	0.0
86-87	7.790249101005254E-5	2.5967497003350846E-5	0.0	2.4557072403613844	0.0
88	7.790249101005254E-5	2.5967497003350846E-5	0.0	2.638739142989503	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13185	0.0	66.11649	1
GTATCAA	27220	0.0	46.311504	1
ATCAACG	36030	0.0	34.736828	3
GTGGTAT	4095	0.0	34.33187	1
TCAACGC	36535	0.0	34.230503	4
TATCAAC	36850	0.0	33.977932	2
CAACGCA	37670	0.0	33.211613	5
TATAACG	340	0.0	33.18911	2
TGGTATC	4130	0.0	33.01499	2
AACGCAG	39025	0.0	32.178864	6
ACGCAGA	43650	0.0	28.715471	7
CGCAGAG	43465	0.0	28.664686	8
GCAGAGT	46040	0.0	26.887943	9
CATGGGT	10990	0.0	26.609299	4
GTACATG	25525	0.0	26.323687	1
TACATGG	24870	0.0	26.240793	2
ACATGGG	24840	0.0	25.325083	3
TACCTGG	2305	0.0	24.68185	2
GAGTACT	32690	0.0	22.700968	12-13
ATGGGTG	6090	0.0	22.69712	5
>>END_MODULE