FastQCFastQC Report
Wed 25 May 2016
SRR2431384_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431384_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617901
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA303581.159631322956827No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29110.1111959543160723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37500.049.81271
GTATCAA70950.039.8568461
TACCTGG24250.033.565922
ATCAACG83300.032.5901763
TCAACGC83450.032.3048364
TATCAAC89850.030.8957062
CAACGCA89400.030.258895
GTACCTG28800.029.2443081
GTACATG161000.028.6405281
AACGCAG95400.028.4743396
TACATGG159650.027.1722892
ACCTGGG29550.026.9082283
CATGGGT73150.025.9840184
ACATGGG163250.025.6214943
CGCAGAG104200.025.5715088
ACGCAGA106600.025.1294827
ATGGGTA29750.023.0881185
GCAGAGT112050.022.6052659
ATGGGTG37650.022.4921115
GTGGTAT17400.021.633241
Produced by FastQC (version 0.11.5)