##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431384_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2617901
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.732107516670798	31.0	31.0	34.0	24.0	34.0
2	29.909122613880356	31.0	31.0	34.0	24.0	34.0
3	29.996414302909088	31.0	31.0	34.0	25.0	34.0
4	33.17354208581608	37.0	35.0	37.0	25.0	37.0
5	33.28608262879307	37.0	35.0	37.0	27.0	37.0
6	33.325617737263556	37.0	35.0	37.0	26.0	37.0
7	33.23721943648748	37.0	35.0	37.0	26.0	37.0
8	33.18893036826068	37.0	35.0	37.0	25.0	37.0
9	34.468641480331	39.0	35.0	39.0	21.0	39.0
10-11	34.499379655685985	39.0	35.0	39.0	23.0	39.0
12-13	34.39911879784606	39.0	35.0	39.0	21.5	39.0
14-15	35.61074635748258	40.0	36.0	41.0	21.5	41.0
16-17	35.612550283605074	40.0	36.0	41.0	22.5	41.0
18-19	35.602536153964564	40.0	36.0	41.0	22.5	41.0
20-21	35.66496899615379	40.0	36.0	41.0	23.5	41.0
22-23	35.637385638341556	40.0	36.0	41.0	24.0	41.0
24-25	35.70375484023269	40.0	36.0	41.0	25.0	41.0
26-27	35.754278141152014	40.0	36.0	41.0	26.0	41.0
28-29	35.723465478641096	40.0	36.0	41.0	26.0	41.0
30-31	35.64915460897872	40.0	36.0	41.0	26.0	41.0
32-33	35.58935593821157	40.0	35.5	41.0	26.0	41.0
34-35	35.50175197610605	40.0	35.0	41.0	25.0	41.0
36-37	35.35115269828768	40.0	35.0	41.0	25.0	41.0
38-39	35.20655441133947	40.0	35.0	41.0	25.0	41.0
40-41	35.12513956792102	40.0	35.0	41.0	24.5	41.0
42-43	34.93599834371124	40.0	35.0	41.0	24.0	41.0
44-45	34.7522450237805	40.0	34.0	41.0	23.5	41.0
46-47	34.598916078186306	39.0	34.0	41.0	23.0	41.0
48-49	34.439695389550636	39.0	34.0	41.0	22.5	41.0
50-51	34.28442481209183	39.0	33.5	41.0	22.0	41.0
52-53	34.10208331025505	39.0	33.0	41.0	20.5	41.0
54-55	33.91088585855615	38.5	33.0	40.5	19.5	41.0
56-57	33.70363757071028	38.0	33.0	40.0	18.0	41.0
58-59	33.467027782945195	38.0	33.0	40.0	16.0	41.0
60-61	33.207871688043205	38.0	32.0	40.0	13.0	41.0
62-63	33.08990924408525	37.0	32.0	40.0	11.0	41.0
64-65	32.83652800468772	37.0	32.0	40.0	8.0	41.0
66-67	32.57089802097177	36.0	32.0	40.0	7.0	41.0
68-69	32.27357031453825	36.0	31.0	39.5	2.0	41.0
70-71	31.94993775547662	35.5	31.0	39.0	2.0	41.0
72-73	31.615846626744098	35.0	31.0	39.0	2.0	40.5
74-75	31.34085952830149	35.0	31.0	38.0	2.0	40.0
76-77	31.093757747141698	35.0	31.0	37.0	2.0	40.0
78-79	30.768829684544983	35.0	31.0	37.0	2.0	39.0
80-81	30.454024044453934	35.0	30.0	36.5	2.0	39.0
82-83	30.14218967791372	35.0	30.0	36.0	2.0	38.5
84-85	29.853532658416036	35.0	30.0	36.0	2.0	37.0
86-87	29.5820437824043	35.0	30.0	35.0	2.0	37.0
88-89	29.32329163707871	34.0	30.0	35.0	2.0	36.5
90-91	29.09531567465691	34.0	29.0	35.0	2.0	36.0
92-93	28.860595759732703	34.0	29.0	35.0	2.0	36.0
94-95	28.634933482969753	34.0	29.0	35.0	2.0	36.0
96-97	28.421636074091417	34.0	29.0	35.0	2.0	35.0
98-99	28.189167199217998	34.0	29.0	35.0	2.0	35.0
100	27.997780282753244	34.0	28.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.47904846497599607
1101	2	0.5311121029759036
1101	3	0.5238922818182203
1101	4	0.5360086949219962
1101	5	0.47757763987215185
1101	6	0.528938691531927
1101	7	0.5058237465502913
1101	8	0.4548244051689352
1101	9	0.5387695781438566
1101	10-11	0.43761349722332454
1101	12-13	0.5189446118422296
1101	14-15	0.6113450683460471
1101	16-17	0.5373207879221127
1101	18-19	0.3570363409626367
1101	20-21	0.3664979480357218
1101	22-23	0.5419016148988902
1101	24-25	0.44135751069498497
1101	26-27	0.4814876996920816
1101	28-29	0.43964094929926745
1101	30-31	0.4399860153226314
1101	32-33	0.4479800208916487
1101	34-35	0.48064582135830847
1101	36-37	0.5773713670352905
1101	38-39	0.5510416458560954
1101	40-41	0.4367369016853857
1101	42-43	0.5624024829720113
1101	44-45	0.4806855578738194
1101	46-47	0.5140858075725419
1101	48-49	0.5480907079178294
1101	50-51	0.483155087172193
1101	52-53	0.45624205706107546
1101	54-55	0.4934344387576246
1101	56-57	0.4726146854168931
1101	58-59	0.5520597592035585
1101	60-61	0.6290123550555862
1101	62-63	0.5973309009065417
1101	64-65	0.6014585891422826
1101	66-67	0.5756555221083843
1101	68-69	0.5436761160533834
1101	70-71	0.5397259490087798
1101	72-73	0.5762255819950681
1101	74-75	0.5653350108684165
1101	76-77	0.6369936802747915
1101	78-79	0.4846891786293419
1101	80-81	0.40712798623972546
1101	82-83	0.5247953172564159
1101	84-85	0.4926432839967454
1101	86-87	0.5308513726131388
1101	88-89	0.4913302066501757
1101	90-91	0.4421275236338573
1101	92-93	0.4720060261408996
1101	94-95	0.45067453919397593
1101	96-97	0.4275766037728772
1101	98-99	0.48065787298511253
1101	100	0.5979681371007608
1102	1	0.15880293391698785
1102	2	0.16103217989435947
1102	3	0.21331634782104203
1102	4	0.35520614134242123
1102	5	0.27805552287079394
1102	6	0.3265716050422611
1102	7	0.07928201934922186
1102	8	0.2719691315770092
1102	9	0.20472102572608009
1102	10-11	0.2324292045891383
1102	12-13	0.17212028178570904
1102	14-15	0.26443884747178004
1102	16-17	0.2689792331041687
1102	18-19	0.28562010996690645
1102	20-21	0.15821815303376496
1102	22-23	0.08405697533042655
1102	24-25	0.14463276046198104
1102	26-27	0.19715990842293962
1102	28-29	0.11630816386852416
1102	30-31	0.2726414664821206
1102	32-33	0.3131290415599466
1102	34-35	0.302028839441455
1102	36-37	0.3181380300492762
1102	38-39	0.3568079671673914
1102	40-41	0.2888563715513257
1102	42-43	0.3061580135479218
1102	44-45	0.2814642717991447
1102	46-47	0.24933316202780986
1102	48-49	0.18699741836956463
1102	50-51	0.09010501064257426
1102	52-53	0.08365385947207926
1102	54-55	0.049858243181141404
1102	56-57	0.08903478118791242
1102	58-59	0.06343278837437083
1102	60-61	0.07701860821486406
1102	62-63	0.1969853982442089
1102	64-65	0.13853752894487315
1102	66-67	0.17678729444954655
1102	68-69	0.10620255382637112
1102	70-71	0.031019747340561565
1102	72-73	0.05975440756291306
1102	74-75	0.219925727631054
1102	76-77	0.12329029765379218
1102	78-79	0.04758972193662103
1102	80-81	-4.765630014027522E-4
1102	82-83	-0.03192026002671611
1102	84-85	-0.062156327993957206
1102	86-87	0.027528019355003153
1102	88-89	-0.039752660965955755
1102	90-91	-0.012958971333095093
1102	92-93	0.004188381085160842
1102	94-95	0.0018147657310763066
1102	96-97	-0.0664905832245104
1102	98-99	-0.13219231336068304
1102	100	-0.20596182680836606
1103	1	-0.16436591410896995
1103	2	0.0077343251981289995
1103	3	-0.026382227985699558
1103	4	0.0888557537455199
1103	5	-0.04522192439799966
1103	6	-6.774304724288527E-4
1103	7	-0.03685729920134406
1103	8	-0.0792013028920735
1103	9	-0.02814632599557143
1103	10-11	-0.013403714105649556
1103	12-13	0.020366180469686412
1103	14-15	-0.044808962155038046
1103	16-17	-0.0956075345179741
1103	18-19	-0.053308103481803926
1103	20-21	-0.15341033083138456
1103	22-23	-0.13738596680045134
1103	24-25	-0.12393965706100829
1103	26-27	-0.12166763799854863
1103	28-29	-0.16877342978570908
1103	30-31	-0.12575276463380192
1103	32-33	-0.15189644969659355
1103	34-35	-0.17657465794452065
1103	36-37	-0.28167787043747694
1103	38-39	-0.15651260250991328
1103	40-41	-0.11021952532986745
1103	42-43	-0.17567115537221412
1103	44-45	-0.28029069484949076
1103	46-47	-0.3694250856736545
1103	48-49	-0.32323434654841066
1103	50-51	-0.4640442592330345
1103	52-53	-0.40415620001954267
1103	54-55	-0.36164137823583786
1103	56-57	-0.32256810325413454
1103	58-59	-0.36789427129752994
1103	60-61	-0.4724059455980054
1103	62-63	-0.3887845675030448
1103	64-65	-0.3703137420123994
1103	66-67	-0.28448957593083435
1103	68-69	-0.29161233710566137
1103	70-71	-0.2692304780064738
1103	72-73	-0.31543607377835414
1103	74-75	-0.2838571459943289
1103	76-77	-0.26794640687117877
1103	78-79	-0.33042585405040015
1103	80-81	-0.3013959789018905
1103	82-83	-0.41112537991133635
1103	84-85	-0.4184092214498776
1103	86-87	-0.4823137689990631
1103	88-89	-0.43555868223871386
1103	90-91	-0.46940036307638167
1103	92-93	-0.5357190283253281
1103	94-95	-0.5658988812853281
1103	96-97	-0.5800717622401983
1103	98-99	-0.6287981183002884
1103	100	-0.7510449347946668
1104	1	-0.3293530125958313
1104	2	-0.27568965498061715
1104	3	-0.2423309050939224
1104	4	-0.2502907996568311
1104	5	-0.19602627799503125
1104	6	-0.2302001983690758
1104	7	-0.18085177756609028
1104	8	-0.3125877996622677
1104	9	-0.3155278320569863
1104	10-11	-0.3253308355993241
1104	12-13	-0.39966390536342544
1104	14-15	-0.387834287347502
1104	16-17	-0.4942649630641185
1104	18-19	-0.4509544710599016
1104	20-21	-0.3934239933391268
1104	22-23	-0.30899892140624274
1104	24-25	-0.324196306915006
1104	26-27	-0.32438410556779473
1104	28-29	-0.2859470604378558
1104	30-31	-0.29124587143850533
1104	32-33	-0.3212813536873824
1104	34-35	-0.22489349698530958
1104	36-37	-0.33654466098698776
1104	38-39	-0.28045795247894745
1104	40-41	-0.2717324887844299
1104	42-43	-0.3772939144200862
1104	44-45	-0.3143046405180243
1104	46-47	-0.32030854731421243
1104	48-49	-0.41098898773059744
1104	50-51	-0.45169331683855773
1104	52-53	-0.4958056823662389
1104	54-55	-0.33329723170013636
1104	56-57	-0.3213496624081884
1104	58-59	-0.49522374656520185
1104	60-61	-0.5478006894259906
1104	62-63	-0.46536823266542626
1104	64-65	-0.43387148026565825
1104	66-67	-0.31249661955529007
1104	68-69	-0.3693166464588167
1104	70-71	-0.4309105063225509
1104	72-73	-0.48030265195727395
1104	74-75	-0.41136278266365167
1104	76-77	-0.33200976409620253
1104	78-79	-0.34378491961981794
1104	80-81	-0.47117298978588096
1104	82-83	-0.39064697976975893
1104	84-85	-0.3662900815507548
1104	86-87	-0.3675275376920979
1104	88-89	-0.42917092894254694
1104	90-91	-0.49106187975631954
1104	92-93	-0.5517582106985728
1104	94-95	-0.5074836259981836
1104	96-97	-0.42012145695747805
1104	98-99	-0.5064559184949644
1104	100	-0.4653615439442973
1105	1	-0.2751015726201622
1105	2	-0.2975072503701526
1105	3	-0.23803726876307252
1105	4	-0.14740272274242017
1105	5	-0.20427450118092594
1105	6	-0.23815069092705698
1105	7	-0.18405854855063808
1105	8	-0.24362788626878285
1105	9	-0.32823641796156267
1105	10-11	-0.37627707867271454
1105	12-13	-0.3636143852079101
1105	14-15	-0.4303806435709703
1105	16-17	-0.41075915693684806
1105	18-19	-0.2581633524840612
1105	20-21	-0.22400155068216066
1105	22-23	-0.3912422916312721
1105	24-25	-0.3473641234847449
1105	26-27	-0.20574960919410756
1105	28-29	-0.1740657428171133
1105	30-31	-0.1808627420761013
1105	32-33	-0.0941096025683521
1105	34-35	-0.2219565076755643
1105	36-37	-0.3458119672620512
1105	38-39	-0.36743975850505706
1105	40-41	-0.3567758328086157
1105	42-43	-0.3685193941367544
1105	44-45	-0.48239911060517926
1105	46-47	-0.4507702184170128
1105	48-49	-0.5665221480406828
1105	50-51	-0.4372675804199133
1105	52-53	-0.4709322541580079
1105	54-55	-0.5612607431499441
1105	56-57	-0.6102282108369863
1105	58-59	-0.5757105475425419
1105	60-61	-0.6260269763111737
1105	62-63	-0.49260827043554656
1105	64-65	-0.47816694346997934
1105	66-67	-0.5722492150077017
1105	68-69	-0.4576566195974152
1105	70-71	-0.4222754198770282
1105	72-73	-0.33335444962426664
1105	74-75	-0.4414322978249743
1105	76-77	-0.4821126397360054
1105	78-79	-0.355447613550659
1105	80-81	-0.5134730221085526
1105	82-83	-0.3175225341712711
1105	84-85	-0.39896289141344
1105	86-87	-0.4748073313797647
1105	88-89	-0.47156527602667353
1105	90-91	-0.4523152338034322
1105	92-93	-0.48765281281258055
1105	94-95	-0.4912345745006661
1105	96-97	-0.5191886805119275
1105	98-99	-0.5339118437904844
1105	100	-0.5643532811420542
1106	1	-0.13796428602451272
1106	2	-0.12676403823780902
1106	3	-0.05956699861234327
1106	4	-0.006439217847798773
1106	5	-0.03337760997418826
1106	6	-0.0957560703435405
1106	7	-0.12067556427384574
1106	8	-0.09041256380956497
1106	9	-0.13004331573525008
1106	10-11	-0.16776996527159582
1106	12-13	-0.14873243735445385
1106	14-15	-0.15712776144275153
1106	16-17	-0.12043131133472684
1106	18-19	-0.14716283086245596
1106	20-21	-0.1460075664796463
1106	22-23	-0.05550807313472461
1106	24-25	-0.03033813073182756
1106	26-27	-0.13952809759322093
1106	28-29	-0.15961762549502367
1106	30-31	-0.07026873173292358
1106	32-33	-0.13501256942863193
1106	34-35	-0.10973175113539924
1106	36-37	-0.20576782210371647
1106	38-39	-0.13504352763498417
1106	40-41	-0.1768602444005083
1106	42-43	-0.15851253585216085
1106	44-45	-0.14166598527602048
1106	46-47	-0.19106181932920663
1106	48-49	-0.12520785625755337
1106	50-51	-0.20718391835285388
1106	52-53	-0.2468259532629844
1106	54-55	-0.28195346167516533
1106	56-57	-0.27887669309556173
1106	58-59	-0.4036533826347579
1106	60-61	-0.32911314478396747
1106	62-63	-0.31161980874784945
1106	64-65	-0.228580340842214
1106	66-67	-0.17198951657358208
1106	68-69	-0.1159407692264054
1106	70-71	-0.30674239185367114
1106	72-73	-0.33687926889410136
1106	74-75	-0.3495309864081335
1106	76-77	-0.3813306178437905
1106	78-79	-0.2998928599013233
1106	80-81	-0.32865367414482805
1106	82-83	-0.2876389727206323
1106	84-85	-0.278701666120444
1106	86-87	-0.2704181020780041
1106	88-89	-0.31473412529815903
1106	90-91	-0.2948512576748712
1106	92-93	-0.2785841517036083
1106	94-95	-0.4143119737978367
1106	96-97	-0.36707496753146884
1106	98-99	-0.4567761052416941
1106	100	-0.4810844497611697
1107	1	-0.29423054359713063
1107	2	-0.3258609892913036
1107	3	-0.26971607061366143
1107	4	-0.15064778585213645
1107	5	-0.12784988467301872
1107	6	-0.23302619543296288
1107	7	-0.2667231509966044
1107	8	-0.09385920969115347
1107	9	-0.1070301558911666
1107	10-11	-0.11228499752810706
1107	12-13	-0.15598217705515083
1107	14-15	-0.17372375716395538
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2216	86-87	0.22917664236744884
2216	88-89	0.3154332094521308
2216	90-91	0.24065227398379818
2216	92-93	0.2830618687958477
2216	94-95	0.311061945910982
2216	96-97	0.26603633863014764
2216	98-99	0.24335360378555393
2216	100	0.24165342122705624
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92336.0
3	18826.0
4	15024.0
5	13588.0
6	8882.0
7	6784.0
8	6958.0
9	7610.0
10	7897.0
11	8527.0
12	9644.0
13	10504.0
14	10878.0
15	10846.0
16	10849.0
17	10381.0
18	10170.0
19	9865.0
20	9434.0
21	9931.0
22	12156.0
23	19248.0
24	31034.0
25	38793.0
26	40515.0
27	31412.0
28	29364.0
29	34486.0
30	40483.0
31	46611.0
32	59233.0
33	78275.0
34	110065.0
35	172329.0
36	273464.0
37	452396.0
38	600318.0
39	258025.0
40	760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62851492756052	21.983856760316435	13.663935310715654	21.723693001407387
2	15.20903992849359	23.32556078657051	39.22628595217397	22.239113332761935
3	17.332274055892192	30.19174146282847	29.633056803752783	22.842927677526557
4	11.674634520769352	20.12027926646254	37.8802778583449	30.32480835442321
5	11.796476162047561	41.413094226692074	33.24672988756477	13.54369972369559
6	27.065616151762224	40.68292388303153	18.852310810413048	13.3991491547932
7	24.765801382446554	36.411204835581216	22.386390829766103	16.43660295220613
8	23.89400454006344	38.088105056716394	20.48425559662771	17.533634806592456
9	25.029792776809103	20.013789908752695	20.624436727327485	34.33198058711071
10-11	22.108600661380564	30.687263168074836	28.02635507138071	19.17778109916389
12-13	22.931533486527236	29.19760121510301	27.41605791425282	20.454807384116926
14-15	21.027669253289087	30.31598304839205	25.26329690962264	23.393050788696225
16-17	20.121070696877965	32.97524309696358	25.951690211907163	20.95199599425129
18-19	20.36127895387162	31.653181378165584	27.834724864756904	20.15081480320589
20-21	22.426229783919027	30.557423809177052	26.928940806271367	20.08740560063255
22-23	21.841059777316495	30.689496277809873	27.040219071305394	20.429224873568234
24-25	21.327329340694792	30.623668844423175	27.174418266856463	20.874583548025573
26-27	20.99307927394493	30.612659137518133	27.24031031457672	21.15395127396022
28-29	21.028242259992393	30.51811560271624	27.459400676193518	20.994241461097847
30-31	21.331707678828366	30.943526918483837	27.007704975435516	20.717060427252278
32-33	20.866165811308466	30.686169802045672	27.51779585926612	20.929868527379742
34-35	21.204281543355556	30.274312651133418	27.438986631071234	21.08241917443979
36-37	21.410303119042148	30.565615262192292	26.95700426375245	21.06707735501311
38-39	21.21442704821147	30.574714093701118	27.247971246519352	20.962887611568064
40-41	21.047489151409323	30.28427283096994	27.5023814911881	21.16585652643264
42-43	21.009075552126987	30.396218704712552	27.43042720196152	21.16427854119894
44-45	21.20606617278098	30.401611070277873	27.342794962251293	21.049527794689855
46-47	21.218405559482356	30.645599055325324	27.16501645496988	20.970978930222444
48-49	21.137772830023355	30.34492236914691	27.300549702822526	21.21675509800721
50-51	21.06064336186161	30.26649882028408	27.300675412212467	21.37218240564184
52-53	21.115449742144637	30.28612091916964	27.30504878905174	21.29338054963398
54-55	21.195927357302775	30.614702058933553	27.161629829296047	21.02774075446763
56-57	21.120335653835575	30.410028025306303	27.00314618132027	21.466490139537854
58-59	21.068427130010342	30.061454763914963	27.368290672183342	21.501827433891354
60-61	21.325109259823826	30.416921916856452	26.964435724259072	21.293533099060646
62-63	21.078577899416313	30.35260097930913	27.150791436205385	21.41802968506917
64-65	21.15038158747269	30.118327475253675	27.333685009241048	21.397605928032583
66-67	20.993353282151414	30.172096781838725	27.112859648291227	21.721690287718634
68-69	21.417614547461696	30.404812661906146	26.91041126501711	21.267161525615048
70-71	21.37305345470759	30.09933661556785	27.097317087667616	21.430292842056943
72-73	21.151356922518875	30.213840737737453	27.354483167731765	21.280319172011904
74-75	21.14341237872372	30.3246125771845	27.18404332499788	21.3479317190939
76-77	21.20064059547321	30.243289047587286	27.07439038212296	21.481679974816547
78-79	21.262763254060236	30.222403237791767	27.288976279230486	21.225857228917505
80-81	21.138181856409837	30.164370914978022	27.25690087379689	21.440546354815247
82-83	21.00840014886195	30.21832511961233	27.44088768205627	21.332387049469453
84-85	21.067055075937642	30.123910585586234	27.220379253363458	21.588655085112666
86-87	21.31488423664575	30.12528868923095	27.13823079732797	21.42159627679532
88-89	21.094054035043186	30.03251705558941	27.349668827589262	21.52376008177814
90-91	21.000173936645012	30.04421049009995	27.194210776808703	21.76140479644634
92-93	21.10683446314435	30.14862896578191	27.12133174550116	21.623204825572586
94-95	20.99355121321361	30.04471072197285	27.365029770835093	21.596708293978445
96-97	20.98934750836965	30.234522237556472	27.156199275385166	21.61993097868871
98-99	20.81644066763166	30.175539732256944	27.22185661665272	21.78616298345868
100	20.92446946955366	30.352099106772833	27.031858588422757	21.691572835250742
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	26045.0
1	19165.0
2	10280.0
3	6790.5
4	4281.5
5	2872.5
6	2365.5
7	2331.0
8	2538.0
9	2501.0
10	2021.5
11	1594.0
12	1495.0
13	1493.0
14	1550.0
15	1663.5
16	1803.5
17	2019.5
18	2449.5
19	3068.0
20	3880.5
21	5208.5
22	7435.5
23	10980.5
24	14850.0
25	18855.5
26	24298.0
27	31110.0
28	39979.5
29	48339.5
30	52870.5
31	57527.5
32	62918.5
33	67848.0
34	72667.0
35	77165.0
36	79603.0
37	80781.5
38	81955.0
39	82197.0
40	83211.5
41	82838.0
42	80512.0
43	79645.5
44	80016.5
45	78912.0
46	77895.0
47	76872.0
48	75628.0
49	75700.5
50	76176.5
51	73766.0
52	69166.5
53	64931.0
54	62203.0
55	61200.0
56	56666.0
57	50266.0
58	46359.0
59	42839.5
60	38432.5
61	33153.0
62	28367.0
63	23535.0
64	18626.0
65	15347.5
66	12617.5
67	9911.0
68	7818.0
69	6237.5
70	4809.5
71	3851.0
72	3103.5
73	2422.0
74	2036.0
75	1684.0
76	1225.5
77	955.0
78	767.5
79	559.5
80	415.0
81	295.0
82	192.5
83	158.5
84	143.5
85	110.0
86	80.0
87	61.0
88	55.5
89	50.0
90	43.0
91	36.0
92	33.0
93	31.0
94	24.0
95	18.0
96	14.5
97	15.0
98	21.0
99	20.0
100	27.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.173688768215452
2	0.16918134031806398
3	0.18331480067428066
4	0.17124406155924154
5	0.18491913941741878
6	0.07311200843729385
7	0.07483094280494182
8	0.06256921098238627
9	0.05710681954741604
10-11	0.0709728901131097
12-13	0.06976963605575612
14-15	0.06677105054774798
16-17	0.06344777743696191
18-19	0.06793610606359829
20-21	0.06957864334823968
22-23	0.06747772356555883
24-25	0.06696204325526443
26-27	0.07051450761507023
28-29	0.06822259512487294
30-31	0.07368498655984317
32-33	0.07039991199056038
34-35	0.07009432365853407
36-37	0.08136289340200413
38-39	0.07674086988010624
40-41	0.07230983906572479
42-43	0.07532752384448456
44-45	0.08588942057014379
46-47	0.07540392092749115
48-49	0.08113370215298439
50-51	0.07704645821213255
52-53	0.07626338811131514
54-55	0.07855530060151243
56-57	0.07152676896490738
58-59	0.07924287434857162
60-61	0.07653077790183815
62-63	0.06764961700232362
64-65	0.07089649303010313
66-67	0.07154586823565903
68-69	0.06883377178892555
70-71	0.06476562711882536
72-73	0.06232092046261489
74-75	0.06749682283631046
76-77	0.07248173250248958
78-79	0.06864277908140912
80-81	0.07710375602438747
82-83	0.07847890351850585
84-85	0.08035063205216698
86-87	0.0757286085302691
88-89	0.07803962029121804
90-91	0.07662627425559637
92-93	0.06705753960902265
94-95	0.06684744763075456
96-97	0.06694294398451278
98-99	0.07630158665281842
100	0.0846861665127902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2617901.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.67032980316164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14125102781826	50.389495539759935
2	13.07333190445241	16.6476670796536
3	3.7493286051902732	7.1616296649867826
4	1.476916967626918	3.7614316168276507
5	0.7267458453792516	2.313607382918723
6	0.4125209766846267	1.5759207981739516
7	0.28018273618913897	1.2487529052820296
8	0.19238978511186183	0.9799616855065313
9	0.13985167198437856	0.8013961870892067
>10	0.735845772744126	8.356242658722046
>50	0.04633916275514301	2.0612617932164703
>100	0.023674554740210012	2.6024075184988784
>500	0.001140696190637163	0.48450856381757523
>1k	4.202564912840082E-4	0.4552660351107006
>5k	0.0	0.0
>10k+	6.003664161200117E-5	1.1604505704358228
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	30358	1.159631322956827	No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2911	0.1111959543160723	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	1.9099270751644162E-5	0.0
18-19	0.0	0.0	0.0	3.8198541503288325E-5	0.0
20-21	0.0	0.0	0.0	1.7189343676479745E-4	0.0
22-23	0.0	0.0	0.0	5.156803102943923E-4	0.0
24-25	0.0	1.9099270751644162E-5	0.0	0.0013560482233667356	0.0
26-27	0.0	3.8198541503288325E-5	0.0	0.001909927075164416	0.0
28-29	0.0	3.8198541503288325E-5	0.0	0.0036670599843156787	0.0
30-31	0.0	3.8198541503288325E-5	0.0	0.008136289340200413	0.0
32-33	0.0	3.8198541503288325E-5	0.0	0.01533671441357026	0.0
34-35	0.0	3.8198541503288325E-5	0.0	0.02587951186847784	0.0
36-37	0.0	3.8198541503288325E-5	0.0	0.04291606137894443	0.0
38-39	0.0	3.8198541503288325E-5	0.0	0.069024764496442	0.0
40-41	0.0	3.8198541503288325E-5	0.0	0.09811295385119606	0.0
42-43	0.0	3.8198541503288325E-5	0.0	0.12588329352408667	0.0
44-45	0.0	3.8198541503288325E-5	0.0	0.15774087713782914	0.0
46-47	0.0	3.8198541503288325E-5	0.0	0.199721074249943	0.0
48-49	0.0	3.8198541503288325E-5	0.0	0.246094103634935	0.0
50-51	0.0	3.8198541503288325E-5	0.0	0.2935557914527708	0.0
52-53	1.9099270751644162E-5	3.8198541503288325E-5	0.0	0.3426218180137446	0.0
54-55	3.8198541503288325E-5	3.8198541503288325E-5	0.0	0.39877367402357844	0.0
56-57	3.8198541503288325E-5	3.8198541503288325E-5	0.0	0.4635965989546587	0.0
58-59	3.8198541503288325E-5	3.8198541503288325E-5	0.0	0.5381983505105808	0.0
60-61	3.8198541503288325E-5	3.8198541503288325E-5	0.0	0.6197522366201014	0.0
62-63	3.8198541503288325E-5	3.8198541503288325E-5	0.0	0.7047057929234146	0.0
64-65	3.8198541503288325E-5	3.8198541503288325E-5	0.0	0.7968215757585944	0.0
66-67	3.8198541503288325E-5	3.8198541503288325E-5	0.0	0.9037965912385533	0.0
68-69	3.8198541503288325E-5	3.8198541503288325E-5	0.0	1.0250769605114938	0.0
70-71	3.8198541503288325E-5	3.8198541503288325E-5	0.0	1.1615221507612397	0.0
72-73	3.8198541503288325E-5	3.8198541503288325E-5	0.0	1.312272694803967	0.0
74-75	3.8198541503288325E-5	3.8198541503288325E-5	0.0	1.4661173207084608	0.0
76-77	3.8198541503288325E-5	3.8198541503288325E-5	0.0	1.6292250929275018	0.0
78-79	3.8198541503288325E-5	3.8198541503288325E-5	0.0	1.8217648413748266	0.0
80-81	3.8198541503288325E-5	3.8198541503288325E-5	0.0	2.0452645077105664	0.0
82-83	3.8198541503288325E-5	3.8198541503288325E-5	0.0	2.280166438685038	0.0
84-85	3.8198541503288325E-5	3.8198541503288325E-5	0.0	2.5157177448650656	0.0
86-87	3.8198541503288325E-5	3.8198541503288325E-5	0.0	2.7664147727511468	0.0
88	3.8198541503288325E-5	3.8198541503288325E-5	0.0	2.9699748004221704	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3750	0.0	49.8127	1
GTATCAA	7095	0.0	39.856846	1
TACCTGG	2425	0.0	33.56592	2
ATCAACG	8330	0.0	32.590176	3
TCAACGC	8345	0.0	32.304836	4
TATCAAC	8985	0.0	30.895706	2
CAACGCA	8940	0.0	30.25889	5
GTACCTG	2880	0.0	29.244308	1
GTACATG	16100	0.0	28.640528	1
AACGCAG	9540	0.0	28.474339	6
TACATGG	15965	0.0	27.172289	2
ACCTGGG	2955	0.0	26.908228	3
CATGGGT	7315	0.0	25.984018	4
ACATGGG	16325	0.0	25.621494	3
CGCAGAG	10420	0.0	25.571508	8
ACGCAGA	10660	0.0	25.129482	7
ATGGGTA	2975	0.0	23.088118	5
GCAGAGT	11205	0.0	22.605265	9
ATGGGTG	3765	0.0	22.492111	5
GTGGTAT	1740	0.0	21.63324	1
>>END_MODULE