##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431384_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2617901 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.58910172691786 34.0 31.0 34.0 31.0 34.0 2 32.66774068232527 34.0 31.0 34.0 31.0 34.0 3 32.76708171928579 34.0 31.0 34.0 31.0 34.0 4 36.26651160605386 37.0 35.0 37.0 35.0 37.0 5 36.18266160561458 37.0 35.0 37.0 35.0 37.0 6 36.23793756906774 37.0 37.0 37.0 35.0 37.0 7 36.18735124055493 37.0 37.0 37.0 35.0 37.0 8 36.20042812925317 37.0 37.0 37.0 35.0 37.0 9 37.97815463609968 39.0 39.0 39.0 35.0 39.0 10-11 37.95367357283564 39.0 38.0 39.0 35.0 39.0 12-13 37.94295353414816 39.0 38.0 39.0 35.0 39.0 14-15 39.36584729521857 41.0 39.0 41.0 36.0 41.0 16-17 39.40214030247897 41.0 39.0 41.0 36.0 41.0 18-19 39.48895183584101 41.0 39.5 41.0 36.0 41.0 20-21 39.46839624569455 41.0 39.0 41.0 36.0 41.0 22-23 39.378473059141655 41.0 39.0 41.0 36.0 41.0 24-25 39.31684372327295 41.0 39.0 41.0 35.5 41.0 26-27 39.17919852584188 41.0 39.0 41.0 35.0 41.0 28-29 39.07522438778243 41.0 39.0 41.0 35.0 41.0 30-31 38.83871468019608 41.0 38.5 41.0 35.0 41.0 32-33 38.54198688185687 40.5 38.0 41.0 35.0 41.0 34-35 38.35435774691251 40.0 38.0 41.0 35.0 41.0 36-37 38.17749200600022 40.0 38.0 41.0 34.0 41.0 38-39 38.01098609152905 40.0 38.0 41.0 34.0 41.0 40-41 37.8448237729387 40.0 37.0 41.0 33.5 41.0 42-43 37.633491106042584 40.0 37.0 41.0 33.0 41.0 44-45 37.32814934560169 40.0 37.0 41.0 33.0 41.0 46-47 36.8755902152144 40.0 37.0 41.0 31.5 41.0 48-49 36.47105696510296 40.0 36.0 41.0 30.0 41.0 50-51 36.28284854927669 40.0 36.0 41.0 30.0 41.0 52-53 36.20863183901912 40.0 35.5 41.0 30.0 41.0 54-55 36.050758985920396 40.0 35.0 41.0 29.5 41.0 56-57 35.88830689166626 40.0 35.0 41.0 29.0 41.0 58-59 35.69782508964243 39.0 35.0 41.0 28.5 41.0 60-61 35.526356038673725 39.0 35.0 41.0 28.0 41.0 62-63 35.34773927661894 39.0 35.0 41.0 28.0 41.0 64-65 35.09878563016707 38.5 35.0 41.0 28.0 41.0 66-67 34.82226677021018 37.5 35.0 41.0 28.0 41.0 68-69 34.523785276830566 37.0 35.0 40.0 27.0 41.0 70-71 34.21876820399244 36.5 35.0 40.0 26.0 41.0 72-73 33.90070308235491 36.0 35.0 39.0 26.0 41.0 74-75 33.57370714171391 36.0 34.0 39.0 26.0 41.0 76-77 33.2542168324929 35.0 34.0 38.5 26.0 40.5 78-79 32.950780415302184 35.0 34.0 37.0 25.5 39.5 80-81 32.70397811070778 35.0 34.0 37.0 25.0 39.0 82-83 32.52666544685991 35.0 34.0 36.5 26.0 39.0 84-85 32.28400138125926 35.0 34.0 36.0 25.0 37.5 86-87 32.0594898355591 35.0 34.0 36.0 25.0 37.0 88-89 31.84145618951977 35.0 34.0 35.5 24.0 37.0 90-91 31.67130976305063 35.0 34.0 35.0 24.0 36.0 92-93 31.519378502090035 35.0 34.0 35.0 23.5 36.0 94-95 31.3798640590305 35.0 34.0 35.0 22.0 36.0 96-97 31.23942234637597 35.0 34.0 35.0 20.0 36.0 98-99 31.12374971398842 35.0 34.0 35.0 18.5 36.0 100 31.018254700999005 35.0 34.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06955967480602965 1101 2 -0.0385151839627369 1101 3 -0.023257833317494203 1101 4 0.01040930671928919 1101 5 0.03964448932871534 1101 6 0.002854144335522335 1101 7 0.04227027270083994 1101 8 0.033076969949078716 1101 9 0.04298122542294891 1101 10-11 0.049628877870262045 1101 12-13 0.05333290477680919 1101 14-15 0.08849633336982521 1101 16-17 0.031692942600109575 1101 18-19 0.0382072251133323 1101 20-21 0.06332034159877509 1101 22-23 0.050508703372777575 1101 24-25 0.10614751173440595 1101 26-27 0.141950254958914 1101 28-29 0.05133862178343662 1101 30-31 0.04728252227138796 1101 32-33 0.027036846249345103 1101 34-35 0.08133769630516241 1101 36-37 0.09659005290213685 1101 38-39 0.06961443999136208 1101 40-41 0.0158934615809585 1101 42-43 -0.024060704573464875 1101 44-45 -0.0393446306833809 1101 46-47 -0.0481355502755747 1101 48-49 -0.02002722106949051 1101 50-51 -0.10605954559864017 1101 52-53 -0.02884980798889103 1101 54-55 -0.05879323856699159 1101 56-57 0.026177021202734352 1101 58-59 -0.02851338009337212 1101 60-61 -0.007777694970535265 1101 62-63 -0.001965721944827692 1101 64-65 -0.011595215214732946 1101 66-67 -0.009598898112820109 1101 68-69 -0.06398448683232516 1101 70-71 0.056820731882773146 1101 72-73 -0.043683528447481024 1101 74-75 0.03539728155134014 1101 76-77 -0.02001357899187184 1101 78-79 -0.004227521434735593 1101 80-81 0.03984045743504794 1101 82-83 0.052627073737284036 1101 84-85 0.0272092658391756 1101 86-87 0.04926196202291777 1101 88-89 -0.029796111850739493 1101 90-91 -0.08972606258788929 1101 92-93 -0.09245192643116695 1101 94-95 -0.1473079485898694 1101 96-97 -0.05759900487186442 1101 98-99 -0.07940092443672953 1101 100 -0.10988630657440623 1102 1 -0.06910883345152286 1102 2 -0.009341432856622589 1102 3 0.010481772734650008 1102 4 0.026309528354730105 1102 5 -0.017353287247210858 1102 6 0.009828266837118349 1102 7 -0.012351197304141692 1102 8 0.009800830576800479 1102 9 0.034723213967659206 1102 10-11 0.053550873626079465 1102 12-13 -0.008570441081701574 1102 14-15 -0.02517401308134737 1102 16-17 -0.029906184761905763 1102 18-19 0.011661639028410775 1102 20-21 0.008354223188185017 1102 22-23 0.05924082731423397 1102 24-25 0.07439758743922198 1102 26-27 0.03411433470053282 1102 28-29 0.027858933651948803 1102 30-31 0.08466590149892994 1102 32-33 0.1360098116749171 1102 34-35 0.13849085923489213 1102 36-37 0.05917515454691369 1102 38-39 0.11376809839000401 1102 40-41 0.04763425416624045 1102 42-43 0.043549518110651775 1102 44-45 0.17438362848279354 1102 46-47 0.12164208375142493 1102 48-49 0.11507237642830859 1102 50-51 0.08614078113983226 1102 52-53 0.13187763874967828 1102 54-55 0.14504331678708127 1102 56-57 0.15197170089180645 1102 58-59 0.11315545167225594 1102 60-61 0.11105449778278143 1102 62-63 0.10509083633138516 1102 64-65 0.15107691751672547 1102 66-67 0.19993164657305584 1102 68-69 0.17740408981099876 1102 70-71 0.08266030423596504 1102 72-73 0.07680363943811841 1102 74-75 0.034807034335329945 1102 76-77 0.08602221613262628 1102 78-79 0.1929142386404905 1102 80-81 0.12086582473597929 1102 82-83 0.09346106101887841 1102 84-85 0.08144492110122314 1102 86-87 0.09110220482035913 1102 88-89 0.07543889123047265 1102 90-91 0.05631236600390821 1102 92-93 0.004367985056049406 1102 94-95 0.03688107014604114 1102 96-97 0.06042083391515973 1102 98-99 0.09828631685603995 1102 100 0.14618377664787374 1103 1 -0.01569257240951316 1103 2 -0.06767422535707368 1103 3 -0.033309249439497535 1103 4 0.04224852240556487 1103 5 0.018222267106494883 1103 6 0.04641972167757302 1103 7 -0.045721884161899595 1103 8 0.023416839230122832 1103 9 0.07268405766696162 1103 10-11 0.09963171011427363 1103 12-13 0.07166471305567512 1103 14-15 0.04208064709531811 1103 16-17 0.024658084212312303 1103 18-19 0.011941429906364931 1103 20-21 -6.247564404446848E-4 1103 22-23 0.008875152174518064 1103 24-25 -0.013381464300451285 1103 26-27 -0.0080048648667983 1103 28-29 -0.02759035425141576 1103 30-31 -0.009455821955192789 1103 32-33 -0.05071086616896281 1103 34-35 -0.1018470531501805 1103 36-37 -0.04969818239197821 1103 38-39 0.0014386229979024279 1103 40-41 0.0032620890319350337 1103 42-43 -8.426217847912199E-4 1103 44-45 -0.10331761543284301 1103 46-47 -0.1370653977701224 1103 48-49 -0.12488190516535269 1103 50-51 -0.170120765642217 1103 52-53 -0.04948706289085436 1103 54-55 -0.056563173207514694 1103 56-57 -0.08753866507177577 1103 58-59 -0.10220030819795056 1103 60-61 -0.05729120259580611 1103 62-63 -0.1576495999404699 1103 64-65 -0.1274126444086363 1103 66-67 -0.025723734714127033 1103 68-69 -0.044793855024046536 1103 70-71 -0.07473547726755925 1103 72-73 -0.08376762430805229 1103 74-75 -0.04714890581249165 1103 76-77 -0.08285061602890664 1103 78-79 -0.07247000509486412 1103 80-81 -0.09178742927520034 1103 82-83 -0.12243632495552248 1103 84-85 -0.11913997163358658 1103 86-87 -0.08269119701847671 1103 88-89 -0.1470022120686032 1103 90-91 -0.1282193087752539 1103 92-93 -0.1614865669540464 1103 94-95 -0.2105364888948813 1103 96-97 -0.20831578047099697 1103 98-99 -0.1717309897962025 1103 100 -0.09099523903628182 1104 1 -7.292948234649543E-4 1104 2 -0.03237858780287439 1104 3 -0.03819462241533955 1104 4 -0.008521214785830011 1104 5 -0.010292094454428025 1104 6 -0.06422556635003929 1104 7 0.009913687911364377 1104 8 -0.056171161831727545 1104 9 -0.036383316901662965 1104 10-11 -0.08933769082369736 1104 12-13 -0.02065235623022943 1104 14-15 -0.12196526034650645 1104 16-17 -0.05027280685927593 1104 18-19 -0.05322678529584124 1104 20-21 -0.05183604747663395 1104 22-23 -0.030976436180488065 1104 24-25 -0.04061859375802612 1104 26-27 -0.018719704261542347 1104 28-29 0.01798062176840176 1104 30-31 0.06016417627505888 1104 32-33 0.009948084366392607 1104 34-35 -0.10091835657465253 1104 36-37 -0.1509261091241214 1104 38-39 -0.16269527665699712 1104 40-41 -0.16705299812849717 1104 42-43 -0.1584856976187865 1104 44-45 -0.11085762074210237 1104 46-47 -0.22386628264756325 1104 48-49 -0.3492849491437582 1104 50-51 -0.39890738629525657 1104 52-53 -0.2931831959764182 1104 54-55 -0.22770116099620452 1104 56-57 -0.2379136761327416 1104 58-59 -0.21358538916423697 1104 60-61 -0.17352438549997373 1104 62-63 -0.2930712794378678 1104 64-65 -0.2633171838299262 1104 66-67 -0.29367849977916904 1104 68-69 -0.34646574246763606 1104 70-71 -0.2601964807185695 1104 72-73 -0.26082891091985516 1104 74-75 -0.19904105694956087 1104 76-77 -0.28794832419632144 1104 78-79 -0.2813009758054221 1104 80-81 -0.27223687622679194 1104 82-83 -0.27113711249645434 1104 84-85 -0.25679016188223613 1104 86-87 -0.3182627393598594 1104 88-89 -0.3109154586839473 1104 90-91 -0.26438800181126965 1104 92-93 -0.3111599055966394 1104 94-95 -0.3154182161756509 1104 96-97 -0.45274052164434053 1104 98-99 -0.42534235162259293 1104 100 -0.3787701262144054 1105 1 -0.17684127968003338 1105 2 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-0.40121061532964575 1105 60-61 -0.45426944309554784 1105 62-63 -0.5033454527948891 1105 64-65 -0.5927282092186275 1105 66-67 -0.5839344582955945 1105 68-69 -0.4570655884961994 1105 70-71 -0.43997274952158705 1105 72-73 -0.3802824256268096 1105 74-75 -0.4696287947592239 1105 76-77 -0.6004958702367773 1105 78-79 -0.4800900640919181 1105 80-81 -0.4434774518328908 1105 82-83 -0.5578152595382093 1105 84-85 -0.5449307750345938 1105 86-87 -0.5147442943636094 1105 88-89 -0.5135381829171202 1105 90-91 -0.5001941829727201 1105 92-93 -0.5798102178303246 1105 94-95 -0.5725144864655931 1105 96-97 -0.5973183832820297 1105 98-99 -0.6021068357097903 1105 100 -0.6136001323210287 1106 1 -0.08070571579966668 1106 2 -0.09080082588280902 1106 3 -0.06130660380225095 1106 4 -9.702005656890833E-4 1106 5 -0.010356923939042417 1106 6 -0.019495923196508613 1106 7 -0.0031816653880554213 1106 8 -0.07516977995074114 1106 9 -0.10083982938468239 1106 10-11 -0.046139306590553986 1106 12-13 -0.0516502704090982 1106 14-15 -0.053470985228941004 1106 16-17 -0.06561088890733657 1106 18-19 -0.09385336361521723 1106 20-21 -0.121141028805404 1106 22-23 -0.1868749029429253 1106 24-25 -0.07960485105530779 1106 26-27 -0.1449879226129127 1106 28-29 -0.13427594739989246 1106 30-31 -0.16441123618397313 1106 32-33 -0.08606468623170827 1106 34-35 -0.06049085879759275 1106 36-37 -0.09797109004906446 1106 38-39 -0.07656015593875765 1106 40-41 -0.11609722581585658 1106 42-43 -0.0789219230652094 1106 44-45 -0.12101224784189668 1106 46-47 -0.18633720307835233 1106 48-49 -0.05857718202450002 1106 50-51 -0.0727932662781896 1106 52-53 -0.07288484123393602 1106 54-55 -0.04470420602358871 1106 56-57 -0.08338230112706668 1106 58-59 -0.05443302252000137 1106 60-61 -0.11196105759453445 1106 62-63 -0.12907817621453432 1106 64-65 -0.09217814936921087 1106 66-67 -0.10314444191330807 1106 68-69 -0.14093071587370076 1106 70-71 -0.11010021906353273 1106 72-73 -0.047377630243779834 1106 74-75 -0.02939664599735181 1106 76-77 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0.03995131017719444 2214 74-75 0.07950710570658259 2214 76-77 0.034184085195001046 2214 78-79 0.06498629440117298 2214 80-81 -0.006394196733793933 2214 82-83 0.009222134367725232 2214 84-85 0.010502073002840007 2214 86-87 -0.004336985355408274 2214 88-89 0.03190318749942733 2214 90-91 0.03364994968406876 2214 92-93 0.06030061807115317 2214 94-95 0.04779497258819987 2214 96-97 0.05027601218559141 2214 98-99 0.05115021970703282 2214 100 0.06997236355551095 2215 1 0.07652727055979369 2215 2 0.06671264116683773 2215 3 0.06364321308726772 2215 4 0.031093062020126183 2215 5 0.014210275671807437 2215 6 0.009201734525532856 2215 7 0.011235717551159041 2215 8 -0.011289798885506741 2215 9 -0.05480373676559225 2215 10-11 0.01272007556752186 2215 12-13 0.018242014982874366 2215 14-15 0.04449834873700098 2215 16-17 0.03340612132865317 2215 18-19 0.057897175802146705 2215 20-21 0.04846960473015116 2215 22-23 -0.0016835915799404688 2215 24-25 -0.014050129539569411 2215 26-27 -0.03582670640854957 2215 28-29 0.055034627904184674 2215 30-31 0.018656669122613323 2215 32-33 0.031756514273581615 2215 34-35 0.010440741282373267 2215 36-37 -0.04559882479026811 2215 38-39 -0.027431612548134865 2215 40-41 -0.053455112966638296 2215 42-43 -0.08140250758064838 2215 44-45 -0.02488763684612394 2215 46-47 0.061031104728094476 2215 48-49 0.15319889426363176 2215 50-51 0.090459105878125 2215 52-53 0.0037177907850960423 2215 54-55 0.021580724008764207 2215 56-57 -0.040670713608541575 2215 58-59 -0.05165983710587341 2215 60-61 -0.014872358912718653 2215 62-63 -3.979225671741915E-4 2215 64-65 -0.05394883284547092 2215 66-67 -0.009119023793353165 2215 68-69 0.08379715139993493 2215 70-71 0.04316259553758783 2215 72-73 0.025418956705514972 2215 74-75 0.05720284293839484 2215 76-77 0.05635052601249413 2215 78-79 0.1299362761328311 2215 80-81 0.12525097186684775 2215 82-83 0.09731556655054163 2215 84-85 0.07703076355556249 2215 86-87 0.1278055909900928 2215 88-89 0.06171042176437069 2215 90-91 0.133392111638738 2215 92-93 0.05468159864195954 2215 94-95 0.017152019313542155 2215 96-97 0.027876939015087032 2215 98-99 0.043356804636342616 2215 100 0.08063645261254138 2216 1 0.005232816268609497 2216 2 0.026104544262807394 2216 3 -0.031599273987588106 2216 4 -0.013036637157028963 2216 5 -0.0252467799812095 2216 6 -0.05320857557750713 2216 7 -0.0330225759717635 2216 8 -0.0693624511068549 2216 9 -0.1877137106883211 2216 10-11 -0.07453756757671925 2216 12-13 -0.1196120555227651 2216 14-15 -0.12949269218906778 2216 16-17 -0.13844851401724867 2216 18-19 -0.17150259300519366 2216 20-21 -0.17151431978195575 2216 22-23 -0.22895125562136087 2216 24-25 -0.14424454545793708 2216 26-27 -0.10956710543810289 2216 28-29 -0.11135909634767671 2216 30-31 -0.15482138023831027 2216 32-33 -0.1250028843196631 2216 34-35 0.0065955266311306104 2216 36-37 -0.02180280189655548 2216 38-39 0.03857917198249794 2216 40-41 -0.009080721352653143 2216 42-43 -0.003896196135748653 2216 44-45 0.0050649777383213745 2216 46-47 0.06510060684194485 2216 48-49 0.07305840577714662 2216 50-51 0.20823549412122588 2216 52-53 0.20477686629162406 2216 54-55 0.21243874600695278 2216 56-57 0.131632353302102 2216 58-59 0.12603740659785956 2216 60-61 0.031178513985317124 2216 62-63 0.0752807578665653 2216 64-65 0.03919007819792597 2216 66-67 0.0458940291778589 2216 68-69 0.0942121704167036 2216 70-71 0.0957267409872955 2216 72-73 0.09196005552531972 2216 74-75 0.04130399904505566 2216 76-77 0.04559574267079114 2216 78-79 0.018101414975056684 2216 80-81 6.290253852228034E-4 2216 82-83 0.07959396590216983 2216 84-85 0.06224465977309279 2216 86-87 0.08409880521317348 2216 88-89 0.11101349871023913 2216 90-91 0.09901990866133303 2216 92-93 0.09454176005629478 2216 94-95 0.01891071782980802 2216 96-97 0.03400423738216318 2216 98-99 0.02795167032430257 2216 100 0.06520560898871253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33.0 3 217.0 4 84.0 5 120.0 6 335.0 7 798.0 8 1376.0 9 2175.0 10 3168.0 11 4880.0 12 7740.0 13 11379.0 14 11124.0 15 13997.0 16 18064.0 17 27766.0 18 31482.0 19 15920.0 20 9673.0 21 8730.0 22 8502.0 23 8357.0 24 8986.0 25 10425.0 26 12504.0 27 15590.0 28 18917.0 29 24035.0 30 30088.0 31 37861.0 32 48345.0 33 62631.0 34 89088.0 35 152709.0 36 235052.0 37 467798.0 38 768769.0 39 445248.0 40 3935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25646886966301 20.431184123268373 14.49177314414417 21.820573862924444 2 14.964068846016326 21.919302738499432 40.55911412095116 22.557514294533085 3 16.58859431958259 29.23853008799899 31.716536070723116 22.456339521695302 4 11.114973408085332 18.399549868386924 39.03459298117079 31.45088374235695 5 11.154004743945775 38.76159857291839 35.09063901874382 14.993757664392012 6 26.69860319393285 39.63625820838909 18.982612405893118 14.682526191784945 7 23.886197377211744 35.20438702609457 23.777713519342406 17.13170207735128 8 25.32295911877493 33.942803795865466 21.55253388115135 19.181703204208254 9 26.345477804911493 15.280421437190665 22.543650461687026 35.83045029621081 10-11 22.971666995810764 27.13838300226021 29.462114877529743 20.42783512439928 12-13 24.97332786839533 25.748739161641332 29.72715545775031 19.55077751221303 14-15 22.108800141792987 26.26573732161759 29.314668507327053 22.310794029262375 16-17 19.20742992191072 29.465724639701808 29.747286089122543 21.579559349264926 18-19 19.141048168379236 26.520378929676458 34.55739333053211 19.7811795714122 20-21 20.899510714881885 25.58620436754484 34.481536925957094 19.032747991616183 22-23 20.35208741659826 25.706854460882973 34.64909482826127 19.2919632942575 24-25 19.926158385316782 25.844832528967395 34.62406229724927 19.60494678846656 26-27 19.61606219952038 25.885023022287346 34.61909134733286 19.879823430859414 28-29 19.633285661462487 25.79840834013269 34.82380507094246 19.744500927462358 30-31 19.96025047624177 26.222767699061805 34.34617075640601 19.470811068290413 32-33 19.595705977985173 25.98664105808076 34.667202980130426 19.750449983803637 34-35 19.95470760006624 25.825912088739027 34.28696175166265 19.932418559532078 36-37 20.235205990506426 26.11006520363554 33.64084732066136 20.01388148519668 38-39 20.030727176743913 26.226489546113303 33.76116075691839 19.981622520224395 40-41 19.973723183150444 26.092355810790114 33.7156885851506 20.218232420908844 42-43 20.02803784727395 26.250243516725302 33.44966748029902 20.272051155701728 44-45 20.381321343047492 26.391450847760627 32.983211333371784 20.244016475820096 46-47 20.52162480036457 26.835796060731187 32.159592222306685 20.48298691659756 48-49 20.67984340101998 26.84845889658761 31.63734096490427 20.834356737488143 50-51 20.654300997023178 26.956621221722965 31.339401572180375 21.049676209073482 52-53 20.712045624703958 27.127370238513603 31.202633428575794 20.957950708206642 54-55 20.79689397112507 27.47728932208987 31.067221849464634 20.658594857320427 56-57 20.65739123459254 27.162353804031746 30.972755288457403 21.207499672918303 58-59 20.525694959331705 26.780242997501418 31.46579665203273 21.228265391134148 60-61 20.810634424746326 27.015570450160826 31.195371972425733 20.978423152667112 62-63 20.624051831341863 26.919079994445877 31.391981780646177 21.06488639356608 64-65 20.646149661880443 26.754858260236126 31.559200431191726 21.039791646691707 66-67 20.588607169432503 26.83032760709762 31.332862471026306 21.248202752443568 68-69 21.078400570229025 27.062768548248012 30.906218372734894 20.95261250878807 70-71 21.06239937048659 26.88165491740501 30.86961088966517 21.18633482244322 72-73 20.885466464112223 26.999725352066946 31.051355343621236 21.063452840199595 74-75 20.880912231365087 27.144376087828032 30.82398276320783 21.15072891759905 76-77 20.926481966429726 27.134039489564366 30.627341229921978 21.312137314083927 78-79 20.989960397382234 27.14474389129382 30.8100153463725 21.055280364951447 80-81 20.80311809079394 27.0885761172 30.80474535922906 21.303560432777005 82-83 20.700753303689623 27.095039342121275 30.964637834176926 21.23956952001218 84-85 20.69208146619906 27.139362481955757 30.677141845415605 21.491414206429578 86-87 20.856671478497837 27.097300726969774 30.549538113866593 21.496489680665796 88-89 20.642507076363607 27.060358230164216 30.728607608475595 21.568527084996582 90-91 20.520379979872992 27.073460903698436 30.572271294032127 21.833887822396445 92-93 20.584573254152076 27.17394314588299 30.46563902676044 21.775844573204495 94-95 20.442555389136352 27.05170238440391 30.69135528004073 21.814386946419003 96-97 20.47521304771249 27.14187462123737 30.503863880183328 21.87904845086681 98-99 20.337473234713443 27.079393704067368 30.531764720263006 22.051368340956184 100 20.462982132993723 27.207477143876503 30.297857288557463 22.031683434572315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3215.0 1 1903.0 2 5206.5 3 8281.0 4 4163.5 5 1861.0 6 3645.0 7 9287.0 8 15785.5 9 16636.0 10 10490.5 11 4849.0 12 3829.0 13 4063.5 14 4519.0 15 4914.0 16 5241.0 17 5570.5 18 6148.0 19 6790.0 20 7537.0 21 8732.5 22 10569.0 23 13833.0 24 17387.5 25 20792.0 26 25922.5 27 32492.0 28 40930.0 29 48653.0 30 52467.0 31 56882.0 32 62018.5 33 66805.0 34 71812.5 35 75801.0 36 78297.0 37 79972.0 38 81093.5 39 81068.5 40 81424.0 41 81029.0 42 79519.5 43 78868.5 44 78802.0 45 78137.0 46 77671.5 47 76615.5 48 75012.5 49 74892.0 50 75457.0 51 72891.0 52 67420.0 53 62772.5 54 60071.0 55 58391.0 56 53584.5 57 47505.0 58 43959.5 59 41157.0 60 37047.0 61 31358.0 62 26415.0 63 21686.5 64 16881.5 65 13651.5 66 11104.0 67 8613.5 68 6603.5 69 5119.0 70 3882.5 71 2967.5 72 2333.0 73 1804.5 74 1561.5 75 1231.0 76 779.5 77 659.0 78 538.0 79 292.0 80 134.5 81 77.5 82 49.0 83 22.0 84 12.5 85 10.5 86 8.0 87 6.0 88 3.5 89 3.5 90 2.0 91 1.5 92 1.5 93 0.5 94 0.5 95 1.0 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005080406019937347 2 0.004469229355884733 3 0.0011459562450986496 4 0.0 5 0.04171280732159085 6 0.0 7 0.0 8 0.0 9 7.639708300657665E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.8198541503288325E-5 20-21 0.0 22-23 0.0 24-25 1.9099270751644162E-5 26-27 1.1459562450986496E-4 28-29 1.909927075164416E-4 30-31 3.055883320263066E-4 32-33 0.0011077577035953615 34-35 8.594671838239873E-4 36-37 9.740628083338523E-4 38-39 8.785664545756314E-4 40-41 1.527941660131533E-4 42-43 4.201839565361716E-4 44-45 1.527941660131533E-4 46-47 1.527941660131533E-4 48-49 1.527941660131533E-4 50-51 3.055883320263066E-4 52-53 8.021693715690548E-4 54-55 9.358642668305638E-4 56-57 0.002043621970425925 58-59 5.347795810460365E-4 60-61 9.740628083338523E-4 62-63 9.740628083338523E-4 64-65 0.0015279416601315329 66-67 3.437868735295949E-4 68-69 2.100919782680858E-4 70-71 2.2919124901972992E-4 72-73 1.527941660131533E-4 74-75 4.7748176879110405E-4 76-77 7.448715593141223E-4 78-79 7.06673017810834E-4 80-81 6.493752055559014E-4 82-83 0.0012414525988568704 84-85 0.0011459562450986496 86-87 0.0018335299921578394 88-89 0.0011841547866019379 90-91 9.740628083338523E-4 92-93 9.740628083338523E-4 94-95 5.347795810460365E-4 96-97 5.729781225493248E-5 98-99 1.7189343676479745E-4 100 1.1459562450986496E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2617901.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.629122859832414 #Duplication Level Percentage of deduplicated Percentage of total 1 75.59054780130595 43.56216966283495 2 15.212399659892368 17.5335449798562 3 4.382809186085952 7.577323471684483 4 1.729650257647159 3.9871290880995565 5 0.872380872791618 2.5137272249337994 6 0.49623477302966174 1.7158544821348456 7 0.32463205581599314 1.309578244020189 8 0.22457109264543404 1.035346807106442 9 0.18248247097173867 0.9464674265456517 >10 0.8872484702338195 9.230509879079001 >50 0.06025609044591249 2.4254881259255345 >100 0.03392830795329949 3.4476374403465946 >500 0.0019281366105830403 0.7943703261591224 >1k 5.98387223971586E-4 0.7997345865107984 >5k 6.648746933017622E-5 0.2288623363946435 >10k+ 2.659498773207049E-4 2.892255918368202 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26708 1.0202066464698245 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21480 0.8205046714906331 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15081 0.5760722044110911 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12215 0.4665951844626669 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5973 0.22815988839914114 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4420 0.16883755344453438 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4353 0.16627825116381406 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3925 0.14992927540040665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.8198541503288325E-5 0.0 5 0.0 0.0 0.0 3.8198541503288325E-5 0.0 6 0.0 0.0 0.0 3.8198541503288325E-5 0.0 7 0.0 0.0 0.0 3.8198541503288325E-5 0.0 8 0.0 0.0 0.0 3.8198541503288325E-5 0.0 9 0.0 0.0 0.0 7.639708300657665E-5 0.0 10-11 0.0 0.0 0.0 1.1459562450986496E-4 0.0 12-13 0.0 0.0 0.0 1.1459562450986496E-4 0.0 14-15 0.0 0.0 0.0 1.1459562450986496E-4 0.0 16-17 0.0 0.0 0.0 1.3369489526150914E-4 0.0 18-19 0.0 0.0 0.0 1.527941660131533E-4 0.0 20-21 0.0 0.0 0.0 2.8648906127466244E-4 0.0 22-23 0.0 0.0 0.0 5.920773933009689E-4 0.0 24-25 0.0 0.0 0.0 0.0014706438478766003 0.0 26-27 0.0 0.0 0.0 0.0020627212411775692 0.0 28-29 0.0 0.0 0.0 0.0038771519625837646 0.0 30-31 0.0 0.0 0.0 0.008460976942978364 0.0 32-33 0.0 0.0 0.0 0.0158714939946163 0.0 34-35 0.0 0.0 0.0 0.02671987978155018 0.0 36-37 0.0 0.0 0.0 0.04404291835329144 0.0 38-39 0.0 0.0 0.0 0.0708009966763449 0.0 40-41 0.0 0.0 0.0 0.10061495831966144 0.0 42-43 0.0 0.0 0.0 0.1290537724688596 0.0 44-45 0.0 0.0 0.0 0.16177082326642606 0.0 46-47 0.0 0.0 0.0 0.20472508318687377 0.0 48-49 0.0 1.9099270751644162E-5 0.0 0.2521294731924546 0.0 50-51 0.0 3.8198541503288325E-5 0.0 0.3008517128798988 0.0 52-53 0.0 3.8198541503288325E-5 0.0 0.3509300007907098 0.0 54-55 0.0 3.8198541503288325E-5 0.0 0.40809411815038077 0.0 56-57 0.0 3.8198541503288325E-5 0.0 0.4744831832830959 0.0 58-59 0.0 3.8198541503288325E-5 0.0 0.5507847699359143 0.0 60-61 0.0 3.8198541503288325E-5 0.0 0.6339620940593247 0.0 62-63 0.0 3.8198541503288325E-5 0.0 0.7208828752500572 0.0 64-65 0.0 3.8198541503288325E-5 0.0 0.8156152581782122 0.0 66-67 0.0 3.8198541503288325E-5 0.0 0.9248248883361135 0.0 68-69 0.0 3.8198541503288325E-5 0.0 1.049103843117062 0.0 70-71 0.0 3.8198541503288325E-5 0.0 1.189903667098183 0.0 72-73 0.0 3.8198541503288325E-5 0.0 1.34506614268454 0.0 74-75 0.0 3.8198541503288325E-5 0.0 1.5027497220101143 0.0 76-77 0.0 7.639708300657665E-5 0.0 1.6705368155633082 0.0 78-79 0.0 7.639708300657665E-5 0.0 1.8676412897202759 0.0 80-81 0.0 7.639708300657665E-5 0.0 2.097004432176771 0.0 82-83 0.0 7.639708300657665E-5 0.0 2.339106788224612 0.0 84-85 0.0 7.639708300657665E-5 0.0 2.5809417544819304 0.0 86-87 0.0 7.639708300657665E-5 0.0 2.840023362227983 0.0 88 0.0 7.639708300657665E-5 0.0 3.04942776674901 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8250 0.0 66.506775 1 TATAACG 335 0.0 47.715355 2 GTATCAA 17120 0.0 47.45576 1 GTGGTAT 2220 0.0 40.451008 1 TACCTGG 2400 0.0 37.610928 2 TGGTATC 2355 0.0 37.531075 2 ATCAACG 22735 0.0 35.62852 3 TATCAAC 22810 0.0 35.55395 2 TCAACGC 23075 0.0 35.123924 4 CAACGCA 23770 0.0 34.11673 5 AACGCAG 24785 0.0 32.994637 6 ATAACGC 515 0.0 32.862663 3 GTACCTG 2810 0.0 32.794327 1 ACCTGGG 2810 0.0 31.620079 3 ACGCAGA 27925 0.0 29.200438 7 GTACACG 355 0.0 29.136894 1 CGCAGAG 27975 0.0 29.097845 8 TACACGG 360 0.0 27.424637 2 GCAGAGT 29660 0.0 27.27048 9 GTACATG 15225 0.0 26.434153 1 >>END_MODULE