FastQCFastQC Report
Wed 25 May 2016
SRR2431383_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431383_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978527
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212830.5349467277713585No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.043.3939251
GTATCAA98650.037.3874321
ATCAACG110600.032.5793233
TCAACGC112000.032.086874
TATCAAC117750.031.0404832
CAACGCA121150.029.7019215
GTACATG210000.027.7337151
AACGCAG129750.027.563246
TACATGG209150.027.0792752
ACATGGG215100.025.3679853
CATGGGT71100.025.0738834
CGCAGAG140500.024.8859988
ACGCAGA146250.024.261357
ATGGGTG38550.021.9631525
GCAGAGT158600.021.3350289
CATGGGG95750.021.1733954
CTGTCGC9950.019.8376459
TCTGTCG9500.019.2929868
AGAGTAC136300.018.32615310-11
CAGAGTA157250.017.87205310-11
Produced by FastQC (version 0.11.5)