FastQCFastQC Report
Wed 25 May 2016
SRR2431383_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431383_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3978527
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262800.6605459759353147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223470.5616902939203379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134190.33728563360258706No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67970.1708421232280188No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49250.12378953316139366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43810.11011613091981026No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.10717534404064619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115050.059.716651
GTATCAA220450.044.4335481
TATCAAC284200.034.2174262
ATCAACG285800.033.9596253
TCAACGC288900.033.6115044
CAACGCA298200.032.5001835
AACGCAG309450.031.3317016
CGCAGAG343950.027.9566758
ACGCAGA347850.027.737817
GTGGTAT41600.026.9039121
GTACATG197700.026.6405491
TACATGG199050.026.010342
TGGTATC42350.025.8716072
GCAGAGT371100.025.6833829
CATGGGT67050.025.0371484
ACATGGG201450.024.9064943
GAGTACT251750.022.95272612-13
AGAGTAC348450.022.28151710-11
TATAACG3400.022.129012
CAGAGTA369900.021.77718410-11
Produced by FastQC (version 0.11.5)