##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431383_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3978527 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.642987216122954 34.0 31.0 34.0 31.0 34.0 2 32.77662989342538 34.0 31.0 34.0 31.0 34.0 3 32.868700149577975 34.0 31.0 34.0 31.0 34.0 4 36.32637053864408 37.0 37.0 37.0 35.0 37.0 5 36.23454962100295 37.0 37.0 37.0 35.0 37.0 6 36.27850910651103 37.0 37.0 37.0 35.0 37.0 7 36.232915096466606 37.0 37.0 37.0 35.0 37.0 8 36.25275258908636 37.0 37.0 37.0 35.0 37.0 9 38.05287157784778 39.0 39.0 39.0 35.0 39.0 10-11 38.03488200532509 39.0 39.0 39.0 35.0 39.0 12-13 38.015513153486204 39.0 39.0 39.0 35.0 39.0 14-15 39.48760031036612 41.0 39.0 41.0 36.0 41.0 16-17 39.516570705690825 41.0 39.0 41.0 36.5 41.0 18-19 39.58856104281811 41.0 40.0 41.0 37.0 41.0 20-21 39.584478376042185 41.0 40.0 41.0 37.0 41.0 22-23 39.51961555118264 41.0 40.0 41.0 36.0 41.0 24-25 39.465904466653114 41.0 39.0 41.0 36.0 41.0 26-27 39.347829359961615 41.0 39.0 41.0 36.0 41.0 28-29 39.26959638579806 41.0 39.0 41.0 36.0 41.0 30-31 39.098111939418786 41.0 39.0 41.0 35.0 41.0 32-33 38.870408445135595 41.0 38.0 41.0 35.0 41.0 34-35 38.73453240357549 41.0 38.0 41.0 35.0 41.0 36-37 38.594402903386104 41.0 38.0 41.0 35.0 41.0 38-39 38.442965197923755 41.0 38.0 41.0 35.0 41.0 40-41 38.304563975561805 41.0 38.0 41.0 35.0 41.0 42-43 38.13223612658655 40.5 38.0 41.0 34.0 41.0 44-45 37.878797353895045 40.0 38.0 41.0 33.5 41.0 46-47 37.49293218319242 40.0 38.0 41.0 33.0 41.0 48-49 37.13710363157018 40.0 38.0 41.0 32.0 41.0 50-51 36.961178848352674 40.0 37.5 41.0 31.0 41.0 52-53 36.89607523085805 40.0 37.0 41.0 31.0 41.0 54-55 36.76289893219274 40.0 37.0 41.0 31.0 41.0 56-57 36.61963749397704 40.0 37.0 41.0 31.0 41.0 58-59 36.45152200801955 40.0 36.0 41.0 31.0 41.0 60-61 36.30692062665403 40.0 36.0 41.0 30.5 41.0 62-63 36.1542930335775 39.5 35.5 41.0 30.5 41.0 64-65 35.927594182470045 39.0 35.0 41.0 30.0 41.0 66-67 35.67246031005948 39.0 35.0 41.0 29.5 41.0 68-69 35.38083717918717 38.5 35.0 41.0 29.0 41.0 70-71 35.07247531561304 37.5 35.0 40.0 29.0 41.0 72-73 34.7477286191598 37.0 35.0 40.0 29.0 41.0 74-75 34.40402779722244 36.5 35.0 39.0 28.5 41.0 76-77 34.06210011896363 36.0 35.0 39.0 28.0 41.0 78-79 33.730010755236805 36.0 35.0 38.5 28.0 40.0 80-81 33.45072824691148 35.0 34.5 37.0 28.0 39.0 82-83 33.235729831668856 35.0 35.0 37.0 29.0 39.0 84-85 32.96015887286928 35.0 34.0 36.5 29.0 38.5 86-87 32.708921291724295 35.0 34.0 36.0 28.5 37.0 88-89 32.47120089922728 35.0 34.0 36.0 27.5 37.0 90-91 32.284420339487454 35.0 34.0 35.5 27.0 37.0 92-93 32.11822516222713 35.0 34.0 35.0 27.0 36.0 94-95 31.97983411951207 35.0 34.0 35.0 27.0 36.0 96-97 31.838830677786024 35.0 34.0 35.0 26.0 36.0 98-99 31.725133447630242 35.0 34.0 35.0 26.0 36.0 100 31.622927279367463 35.0 34.0 35.0 25.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.035442254354414615 1101 2 0.0342221268857017 1101 3 0.04445336645482456 1101 4 0.07299906567187264 1101 5 0.04854591694141419 1101 6 0.07561231649523847 1101 7 0.07289550264824385 1101 8 0.12031916638868978 1101 9 0.046921235932593675 1101 10-11 0.09593002956241747 1101 12-13 0.11848362278377067 1101 14-15 0.09576695922857681 1101 16-17 0.07067369613572794 1101 18-19 0.08048300166183964 1101 20-21 0.13330970073436532 1101 22-23 0.07684893654128899 1101 24-25 0.14495267465457573 1101 26-27 0.1378477781659413 1101 28-29 0.07818145728178649 1101 30-31 0.09167481077147954 1101 32-33 0.11504184537268003 1101 34-35 0.09923779733559002 1101 36-37 0.05286011001039981 1101 38-39 0.024178332531846536 1101 40-41 0.027717909951462616 1101 42-43 0.019840847262450723 1101 44-45 -0.00372755282657522 1101 46-47 0.05306047361330002 1101 48-49 0.11748239268593608 1101 50-51 0.0337598708975122 1101 52-53 0.041521934114932435 1101 54-55 0.03790439537192469 1101 56-57 0.07951508086544834 1101 58-59 0.016506583166915334 1101 60-61 0.03497962857127135 1101 62-63 0.056070404822122555 1101 64-65 0.06760672757942388 1101 66-67 0.0530583829716349 1101 68-69 0.030448479248427418 1101 70-71 0.061165529443151456 1101 72-73 0.052777403244363086 1101 74-75 0.0256673818660218 1101 76-77 0.05688751498290401 1101 78-79 0.05028220993944643 1101 80-81 0.05083975359159609 1101 82-83 0.023333191337528092 1101 84-85 0.031056553100327733 1101 86-87 0.06226151101456878 1101 88-89 0.10784947579841031 1101 90-91 0.07453843791394377 1101 92-93 0.03390508123601421 1101 94-95 0.033737107256737175 1101 96-97 0.08207539927979468 1101 98-99 0.1297125072433154 1101 100 0.17783212282320093 1102 1 -0.03531971671417722 1102 2 -8.120519607857091E-4 1102 3 0.009955333678909994 1102 4 0.050183157985820515 1102 5 -0.009967121221102104 1102 6 -0.04653107483410679 1102 7 -0.07676962950356625 1102 8 -0.015520901201220738 1102 9 -0.014834840446440012 1102 10-11 -0.03807036432309019 1102 12-13 -0.033417458204141326 1102 14-15 -0.03979754057749574 1102 16-17 -0.011762424244764702 1102 18-19 0.0038786801308532404 1102 20-21 0.006573719902050357 1102 22-23 0.0042222782389416125 1102 24-25 0.011949423702645845 1102 26-27 0.007691569606244286 1102 28-29 -0.015479530941313158 1102 30-31 -0.021737757105647404 1102 32-33 -9.792674115871591E-4 1102 34-35 -0.01466503261319474 1102 36-37 -0.05760798374328857 1102 38-39 -0.04541892668049741 1102 40-41 -0.09746893894031672 1102 42-43 -0.08176472960012404 1102 44-45 -0.10831146207624442 1102 46-47 -0.1479160657444325 1102 48-49 -0.12247832179728846 1102 50-51 -0.11992340853879568 1102 52-53 -0.06841940140022729 1102 54-55 -0.07638105468011958 1102 56-57 -0.11749215896855247 1102 58-59 -0.10673222074942146 1102 60-61 -0.16384220936949134 1102 62-63 -0.0572522415894241 1102 64-65 -0.09392490358013106 1102 66-67 -0.09048060688274262 1102 68-69 -0.12198313062791044 1102 70-71 -0.1925330436249979 1102 72-73 -0.13726792896727602 1102 74-75 -0.12194850292922865 1102 76-77 -0.14549622160514275 1102 78-79 -0.0897020811917244 1102 80-81 -0.07844631931595103 1102 82-83 -0.0838137977814668 1102 84-85 -0.08290342624582081 1102 86-87 -0.0915132105609544 1102 88-89 -0.12000164913696665 1102 90-91 -0.1481625068464325 1102 92-93 -0.1114755995428851 1102 94-95 -0.13081889545962255 1102 96-97 -0.12080761480207514 1102 98-99 -0.14804490695609118 1102 100 -0.11588977303349779 1103 1 -0.10354402910409988 1103 2 -0.10095323565809622 1103 3 -0.08552284009429911 1103 4 -0.030675127569537608 1103 5 -0.054354489179957 1103 6 -0.04975416821665135 1103 7 -0.07117440275685993 1103 8 -1.0096271348913888E-4 1103 9 0.02008331428981336 1103 10-11 0.006102431004471498 1103 12-13 -0.018692162889550445 1103 14-15 -0.003870058920611541 1103 16-17 -0.024735076142889056 1103 18-19 -0.08561621595718805 1103 20-21 -0.05624703053870661 1103 22-23 -0.06933896924807215 1103 24-25 -0.07567793259325128 1103 26-27 -0.06907442946558007 1103 28-29 -0.021313480611851787 1103 30-31 -0.020101702914935515 1103 32-33 -0.03017867946766728 1103 34-35 -0.026494669025950657 1103 36-37 -0.004826300244566539 1103 38-39 -0.10565924554263972 1103 40-41 -0.09686791503624903 1103 42-43 -0.11977499150204096 1103 44-45 -0.11930102551568211 1103 46-47 -0.07213218374596408 1103 48-49 -0.020107235204655183 1103 50-51 -0.04324765856660662 1103 52-53 -0.06282114616023904 1103 54-55 -0.045260894534635554 1103 56-57 -0.07792755007143626 1103 58-59 -0.12160356709358666 1103 60-61 -0.09602136993763111 1103 62-63 -0.11509105846798207 1103 64-65 -0.09574015138837666 1103 66-67 -0.008339047776516395 1103 68-69 -0.03104597285735622 1103 70-71 -0.040359860526045566 1103 72-73 -0.060832454992393536 1103 74-75 -0.03836597119147456 1103 76-77 -0.019827510876353927 1103 78-79 0.02317472432996226 1103 80-81 0.06792976854600852 1103 82-83 0.024634760402584277 1103 84-85 0.014606928638613681 1103 86-87 0.012527802754881634 1103 88-89 0.004516467107912092 1103 90-91 -0.015968526851203535 1103 92-93 -0.04146491098060778 1103 94-95 -0.022745668542260233 1103 96-97 -0.005202509576221814 1103 98-99 -0.053384022568341294 1103 100 -0.03778604965476973 1104 1 -0.11736440837898954 1104 2 -0.07277842735447848 1104 3 -0.10560964209604862 1104 4 -0.02469026574943456 1104 5 -0.056235411616498254 1104 6 -0.10982381372072325 1104 7 -0.06815329803452386 1104 8 -0.10326575509517966 1104 9 -0.09829373200485492 1104 10-11 -0.09599008027189626 1104 12-13 -0.08051608815922862 1104 14-15 -0.10612047528825741 1104 16-17 -0.09236027118454615 1104 18-19 -0.08418531307459887 1104 20-21 -0.08267333366391227 1104 22-23 -0.11651655015003115 1104 24-25 -0.04468711697462169 1104 26-27 -0.08973649982633702 1104 28-29 -0.13235301781652709 1104 30-31 -0.05946885594786977 1104 32-33 -0.04411693997084143 1104 34-35 -0.053662707920388186 1104 36-37 -0.054936973195047756 1104 38-39 -0.12370064893458732 1104 40-41 -0.12286251798757775 1104 42-43 -0.08642595394651664 1104 44-45 -0.10181756672113806 1104 46-47 -0.008263258113885286 1104 48-49 -0.07617230404967756 1104 50-51 -0.12178184531755676 1104 52-53 -0.09408954021170501 1104 54-55 -0.019432161622439992 1104 56-57 -0.0974464512909492 1104 58-59 -0.05167212139980393 1104 60-61 -0.06916237102006306 1104 62-63 -0.08517232483097814 1104 64-65 -0.026861870925046105 1104 66-67 -0.0416882844557378 1104 68-69 -0.10848298804093304 1104 70-71 -0.06593351598311159 1104 72-73 -0.08496863648487363 1104 74-75 -0.033194131423918805 1104 76-77 -0.05861601044744447 1104 78-79 -0.012549062429265234 1104 80-81 -0.05881226511227311 1104 82-83 -0.0581548988649061 1104 84-85 -0.07555608198423869 1104 86-87 -0.06653306617314314 1104 88-89 -0.11371024079594605 1104 90-91 -0.1464777817223677 1104 92-93 -0.13619183672579283 1104 94-95 -0.12871611380674253 1104 96-97 -0.10250350734524005 1104 98-99 -0.07903155291678488 1104 100 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-0.0684716023273495 1105 56-57 -0.046418164819058916 1105 58-59 -0.05479161995159387 1105 60-61 -0.05301759308543552 1105 62-63 -0.06316901734444258 1105 64-65 -0.0874457140437741 1105 66-67 -0.11990619672194214 1105 68-69 -0.005628678906049345 1105 70-71 0.01729021259170338 1105 72-73 0.05013177987945028 1105 74-75 -0.018312029053539902 1105 76-77 -0.011795354934136526 1105 78-79 0.01305581090911545 1105 80-81 0.004775223500054437 1105 82-83 -0.0010360772854411948 1105 84-85 0.006839068153425387 1105 86-87 0.018324373180227838 1105 88-89 0.07890548470111725 1105 90-91 0.12364127413839299 1105 92-93 0.11447261904091732 1105 94-95 0.05867550414514611 1105 96-97 0.11316799482665374 1105 98-99 0.13098899101347072 1105 100 0.18598014652469175 1106 1 -0.18374893605535192 1106 2 -0.1302154193297369 1106 3 -0.11876429138922617 1106 4 -0.06079224050274945 1106 5 -0.11804746289482893 1106 6 -0.14795310791754446 1106 7 -0.10288541227219383 1106 8 -0.08915658002980109 1106 9 -0.14230642034743823 1106 10-11 -0.15332649600218673 1106 12-13 -0.17373228146703212 1106 14-15 -0.16261550065394914 1106 16-17 -0.18034941159721996 1106 18-19 -0.11828899857998465 1106 20-21 -0.14320260030705612 1106 22-23 -0.14284020206510917 1106 24-25 -0.09767119460354223 1106 26-27 -0.12847673698920659 1106 28-29 -0.16843671266451565 1106 30-31 -0.24558905766390637 1106 32-33 -0.14558035595366192 1106 34-35 -0.18108673660445618 1106 36-37 -0.2444523548162323 1106 38-39 -0.22942038572042378 1106 40-41 -0.23666104643020702 1106 42-43 -0.2677068632369526 1106 44-45 -0.27701335583097375 1106 46-47 -0.32939004618421563 1106 48-49 -0.3114551713929856 1106 50-51 -0.39550063278363723 1106 52-53 -0.36153461416987653 1106 54-55 -0.3313798783284341 1106 56-57 -0.37499915109619053 1106 58-59 -0.34055039170133483 1106 60-61 -0.3019968340211534 1106 62-63 -0.36302470919829943 1106 64-65 -0.3659269521528259 1106 66-67 -0.4271672806239266 1106 68-69 -0.4286238230199473 1106 70-71 -0.34874199004720907 1106 72-73 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2213 48-49 0.1562744615249514 2213 50-51 0.23942329853012723 2213 52-53 0.16383730529550888 2213 54-55 0.23844649418207808 2213 56-57 0.21879964453181344 2213 58-59 -0.22150937516677516 2213 60-61 -0.13913443747642162 2213 62-63 0.1688547863712344 2213 64-65 0.1976717385207749 2213 66-67 0.20937092314895267 2213 68-69 0.2155848168024619 2213 70-71 0.17892808633299495 2213 72-73 0.19078997014193533 2213 74-75 0.18159148157768357 2213 76-77 0.18800026625694954 2213 78-79 0.1919414426024204 2213 80-81 0.21638216655856723 2213 82-83 0.19017637492311934 2213 84-85 0.1587549116400382 2213 86-87 -0.25257236249324677 2213 88-89 -0.2773275329611735 2213 90-91 0.07816849787547397 2213 92-93 0.17134736370427106 2213 94-95 0.2085938015117712 2213 96-97 0.22204674828168436 2213 98-99 0.26014181776773526 2213 100 0.2717389848775369 2214 1 0.14716182585969761 2214 2 0.10720091311237923 2214 3 0.09729167494919011 2214 4 0.06128258492930172 2214 5 0.09693144779762264 2214 6 0.06338503844011001 2214 7 0.07252240343522232 2214 8 0.060309466938662126 2214 9 0.10817247730765445 2214 10-11 0.07014793439187628 2214 12-13 0.046944248905546715 2214 14-15 0.15566051120444513 2214 16-17 0.1662824873122446 2214 18-19 0.14223306492749543 2214 20-21 0.1255191817465331 2214 22-23 0.13034241485226516 2214 24-25 0.1487882880757354 2214 26-27 0.1705202846969769 2214 28-29 0.17477508251694474 2214 30-31 0.18166943941608338 2214 32-33 0.17028747039035608 2214 34-35 0.1021090491504495 2214 36-37 0.17371016422453778 2214 38-39 0.16525211483936886 2214 40-41 0.15002016056580914 2214 42-43 0.1051154268737946 2214 44-45 0.09297151516471303 2214 46-47 0.15131479337775744 2214 48-49 0.21669061321113503 2214 50-51 0.19896021367439687 2214 52-53 0.10904756498349855 2214 54-55 0.12856981450681815 2214 56-57 0.07959676530853699 2214 58-59 0.04543329835924226 2214 60-61 0.10277771633382571 2214 62-63 0.03524243775281377 2214 64-65 0.0714533666034427 2214 66-67 0.011476329356959525 2214 68-69 0.03833724033673036 2214 70-71 0.0507170572066471 2214 72-73 0.044136603626057536 2214 74-75 0.07299193911041613 2214 76-77 0.036030475747722335 2214 78-79 0.040348886213827484 2214 80-81 0.0615547784411703 2214 82-83 0.012480494059197156 2214 84-85 0.015646213020417576 2214 86-87 -0.006609192061553415 2214 88-89 -0.004891202587039345 2214 90-91 0.0029613793209364303 2214 92-93 0.023282355699791424 2214 94-95 0.010561700773951799 2214 96-97 0.06651729815199303 2214 98-99 0.054823134214796454 2214 100 0.10966006304086662 2215 1 0.1113507453130822 2215 2 0.09364047366447892 2215 3 0.0734263488303526 2215 4 0.043700369322067445 2215 5 0.040084497337041114 2215 6 0.029467863246857462 2215 7 0.020057402425955217 2215 8 0.010807758831234082 2215 9 0.0043632332426497555 2215 10-11 0.05973761193395433 2215 12-13 0.052387614993008924 2215 14-15 0.07514075962576072 2215 16-17 0.06111124340359453 2215 18-19 0.04706680859177936 2215 20-21 0.06496077134670486 2215 22-23 0.033422796206416194 2215 24-25 0.05960570959194911 2215 26-27 0.08597896426486784 2215 28-29 0.1401799133376045 2215 30-31 0.0905589781399101 2215 32-33 0.10068369148551426 2215 34-35 0.07083903570811145 2215 36-37 -0.00811551924076781 2215 38-39 0.01872075010786034 2215 40-41 0.003961546601935595 2215 42-43 0.050405282103994864 2215 44-45 0.10268566290802994 2215 46-47 0.16128101474510714 2215 48-49 0.22311358265645964 2215 50-51 0.23501239099699234 2215 52-53 0.13525770754549882 2215 54-55 0.1444525051493315 2215 56-57 0.13355000509673687 2215 58-59 0.20854179744350176 2215 60-61 0.21431754298172478 2215 62-63 0.2061630010087825 2215 64-65 0.1452733746133319 2215 66-67 0.15240164468635697 2215 68-69 0.14915963304871127 2215 70-71 0.08204546872447338 2215 72-73 0.12267810744096863 2215 74-75 0.17530702581269253 2215 76-77 0.16222692368115332 2215 78-79 0.17659332733170885 2215 80-81 0.16453814684755486 2215 82-83 0.1503986712863039 2215 84-85 0.15306135208609817 2215 86-87 0.17823655502966318 2215 88-89 0.08021659161565964 2215 90-91 0.09287221310925986 2215 92-93 0.08694398012694649 2215 94-95 0.1113701088364003 2215 96-97 0.12287810588299664 2215 98-99 0.13574085095328314 2215 100 0.1387997491021835 2216 1 0.14027429375943257 2216 2 0.12373899031933888 2216 3 0.06595584628023943 2216 4 0.057821284815204876 2216 5 0.015321323860746361 2216 6 0.03511321755213004 2216 7 0.08062346051361402 2216 8 0.036785463174553 2216 9 0.015827951483366576 2216 10-11 0.02344633446445954 2216 12-13 0.007642772862880065 2216 14-15 0.03974046781423368 2216 16-17 0.04745500454820473 2216 18-19 0.030748989941528748 2216 20-21 0.06747075581719031 2216 22-23 -0.020211159311905647 2216 24-25 0.034752227935172186 2216 26-27 0.09039053102601002 2216 28-29 0.055838990405675304 2216 30-31 -0.0068789336015129265 2216 32-33 -1.8781597555772578E-4 2216 34-35 -0.0022560460879788025 2216 36-37 0.006981715611154016 2216 38-39 0.04064069755558819 2216 40-41 0.02068746689962353 2216 42-43 0.08572543815731137 2216 44-45 0.09121878776403491 2216 46-47 0.13692219262493666 2216 48-49 0.13406619959127397 2216 50-51 0.08026895940389522 2216 52-53 0.021290503473743172 2216 54-55 0.056069545454789704 2216 56-57 0.09513868601027298 2216 58-59 0.08829228220751872 2216 60-61 -0.0028323972982846612 2216 62-63 0.026605553259614112 2216 64-65 0.048922483834623165 2216 66-67 0.0259810542579757 2216 68-69 0.021432785068867588 2216 70-71 0.003997563746985122 2216 72-73 0.006923588373950906 2216 74-75 0.03411876952540638 2216 76-77 0.030090811580166132 2216 78-79 0.03110439048702318 2216 80-81 0.054371493812126914 2216 82-83 0.06183889410102239 2216 84-85 0.07618895940083092 2216 86-87 0.06016464741057348 2216 88-89 0.07296143934207322 2216 90-91 0.10279178218563345 2216 92-93 0.09380795564287325 2216 94-95 0.08704256181391301 2216 96-97 0.05173674125614269 2216 98-99 0.038172193952615174 2216 100 0.06726317566872808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37.0 3 255.0 4 52.0 5 133.0 6 296.0 7 751.0 8 1469.0 9 2514.0 10 3671.0 11 5230.0 12 8398.0 13 12511.0 14 14271.0 15 18614.0 16 24041.0 17 37052.0 18 44935.0 19 22583.0 20 12507.0 21 10160.0 22 9776.0 23 9649.0 24 10822.0 25 12774.0 26 15678.0 27 19890.0 28 25332.0 29 31383.0 30 40253.0 31 50884.0 32 64489.0 33 84503.0 34 116995.0 35 187541.0 36 295570.0 37 643772.0 38 1283784.0 39 847569.0 40 8383.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.93324106644171 20.968304663734436 14.473141134661569 22.62531313516228 2 14.52427801400185 22.099462789933067 40.644973823200274 22.73128537286481 3 16.69472588846522 28.26441690188991 31.825466445812356 23.21539076383251 4 11.013573616567136 18.461154090446037 40.058644820055264 30.466627472931563 5 11.051010544506104 38.98404392823338 35.82906594163757 14.135879585622938 6 27.269514571598986 40.00475552886785 18.64016506611618 14.085564833416992 7 23.724257746648444 35.10927536749154 23.819192379491202 17.347274506368816 8 24.34139569745285 34.96610680284437 21.791230774605776 18.901266725097003 9 25.462734421106926 15.720980946702849 22.867274396233377 35.949010235956855 10-11 22.31461543430521 27.601660111895683 29.980304268388778 20.10342018541033 12-13 24.24173569765896 25.995525479656163 29.981900336481317 19.78083848620356 14-15 21.572735534122998 27.019174058536493 28.937723551671706 22.4703668556688 16-17 18.854892778156337 30.35522946055161 29.45740973983587 21.332468021456183 18-19 18.682064167255977 27.936939938411843 33.764778877194296 19.616217017137885 20-21 20.77796631768491 26.96254166428932 33.29090138134038 18.968590636685388 22-23 20.04886733819669 27.201867324498153 33.48219497846753 19.267070358837625 24-25 19.6575265167234 27.296974483269814 33.547855776773666 19.497643223233123 26-27 19.381557157684966 27.129316408633404 33.71504938577103 19.774077047910602 28-29 19.304212075554123 27.21280786060062 33.785896307564194 19.697083756281064 30-31 19.759435488147968 27.74371706844535 33.21632155901307 19.280525884393608 32-33 19.23609693762134 27.402201244898798 33.80638716935832 19.55531464812154 34-35 19.481675859841474 27.159956214598736 33.61981100893679 19.738556916622997 36-37 19.86643914293025 27.51148301137669 32.97969378301193 19.64238406268113 38-39 19.72245538545915 27.56922811650786 33.122860136451074 19.58545636158191 40-41 19.601067783865886 27.405979182462424 33.082109291632406 19.910843742039283 42-43 19.539591975849277 27.417972210482077 32.85010020699937 20.192335606669275 44-45 20.077563960293805 27.577369792759775 32.38153958139742 19.963526665549 46-47 20.189384304329945 28.04728587787833 31.69368841308988 20.069641404701844 48-49 20.23781441477332 28.012396561788954 31.237098696815213 20.51269032662251 50-51 20.3204410890895 28.099007268036623 30.90600867258043 20.674542970293448 52-53 20.33086334914373 28.220129669457123 30.764517938353908 20.684489043045236 54-55 20.519199364784285 28.58013251758016 30.53980768085186 20.360860436783696 56-57 20.355222281304588 28.13191657983402 30.583091833642772 20.929769305218617 58-59 20.156312366344366 27.77915741277886 31.107477979182146 20.957052241694626 60-61 20.403518124873223 28.16134943896958 30.648930760795007 20.786201675362193 62-63 20.189224653471655 28.104610319697244 30.8161734885661 20.889991538265 64-65 20.13106307808085 27.855660950728243 31.007642141468594 21.00563382972231 66-67 20.060751272301804 27.975696977580217 30.693015677322016 21.27053607279596 68-69 20.7550520350467 28.085905300726736 30.422653345634387 20.736389318592174 70-71 20.64013275293303 27.889225599931233 30.428789007767588 21.041852639368148 72-73 20.42081948501994 27.97538181883076 30.732749314255564 20.871049381893734 74-75 20.552365359074308 28.106290096689513 30.384443112845318 20.95690143139086 76-77 20.670745830444375 28.095130553223235 30.178761579550546 21.055362036781847 78-79 20.824365345457828 28.12709956483632 30.37024227454397 20.678292815161882 80-81 20.666727242131063 27.969891229338835 30.433653717215886 20.929727811314216 82-83 20.401809183179356 28.03135518698659 30.73550316509029 20.831332464743763 84-85 20.37393071895852 28.05709501416492 30.46034287874473 21.108631388131833 86-87 20.56011070605524 28.21831845384531 30.226304702808005 20.995266137291445 88-89 20.339570957933546 27.967777230110613 30.34915750720562 21.34349430475022 90-91 20.154747172262237 27.926591235191147 30.381379666830842 21.537281925715774 92-93 20.291024767418218 28.170377577640576 30.22476098361867 21.313836671322534 94-95 20.022503436279496 27.94540711099035 30.447616798627923 21.58447265410223 96-97 19.92303930766293 28.064587763629454 30.373999204981644 21.63837372372597 98-99 19.991439023274495 27.977741963213436 30.25751045992503 21.77330855358704 100 20.092144701868158 28.106295674904647 29.993854506381133 21.80770511684607 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3515.0 1 2012.0 2 3091.5 3 4914.0 4 2522.5 5 1215.0 6 2878.5 7 7074.0 8 12081.0 9 13318.5 10 8557.0 11 3995.0 12 3492.5 13 3948.5 14 4477.0 15 5114.5 16 5706.0 17 6416.5 18 7307.0 19 8277.5 20 9598.0 21 11627.5 22 15101.5 23 20426.5 24 26099.0 25 32091.5 26 40684.5 27 51577.5 28 66239.0 29 80333.5 30 89955.0 31 100615.0 32 111550.5 33 121424.0 34 131342.0 35 138651.5 36 142889.5 37 145913.0 38 146899.5 39 146096.5 40 144860.5 41 141050.0 42 134894.0 43 129753.0 44 124961.5 45 121159.5 46 118502.0 47 115664.5 48 113602.0 49 110870.0 50 106622.0 51 99909.5 52 92304.5 53 85832.5 54 81098.0 55 77813.5 56 71830.0 57 64737.5 58 57975.0 59 50884.5 60 42889.5 61 34546.5 62 27868.5 63 21845.0 64 16908.0 65 13210.0 66 10008.0 67 7571.5 68 5828.0 69 4331.5 70 3218.5 71 2336.5 72 1723.0 73 1333.0 74 1017.5 75 755.5 76 502.5 77 343.5 78 261.5 79 175.5 80 105.0 81 60.5 82 28.0 83 17.0 84 14.0 85 11.5 86 7.5 87 5.0 88 2.5 89 2.0 90 1.0 91 1.5 92 1.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005353740215914081 2 0.0049013114652734545 3 9.29992431872399E-4 4 0.0 5 0.04672583596894026 6 0.0 7 0.0 8 0.0 9 7.540479177343775E-5 10-11 0.0 12-13 0.0 14-15 1.2567465295572959E-5 16-17 0.0 18-19 6.28373264778648E-5 20-21 0.0 22-23 1.2567465295572959E-5 24-25 0.0 26-27 6.28373264778648E-5 28-29 3.770239588671887E-5 30-31 2.513493059114592E-4 32-33 0.0012818814601484418 34-35 8.043177789166694E-4 36-37 0.0010305321542369826 38-39 7.917503136210965E-4 40-41 1.6337704884244846E-4 42-43 3.1418663238932395E-4 44-45 1.0053972236458367E-4 46-47 7.540479177343775E-5 48-49 1.1310718766015663E-4 50-51 2.764842365026051E-4 52-53 6.660756606653668E-4 54-55 9.048575012812531E-4 56-57 0.0022244413573164136 58-59 4.7756368123177246E-4 60-61 0.0011059369460104205 62-63 7.163455218476587E-4 64-65 0.001533230766059901 66-67 2.3878184061588623E-4 68-69 1.6337704884244846E-4 70-71 2.136469100247403E-4 72-73 1.0053972236458367E-4 74-75 4.7756368123177246E-4 76-77 3.895914241627617E-4 78-79 6.03238334187502E-4 80-81 4.649962159361995E-4 82-83 0.0011687742724882852 84-85 9.048575012812531E-4 86-87 0.001596068092537766 88-89 9.048575012812531E-4 90-91 9.42559897167972E-4 92-93 8.545876400989612E-4 94-95 5.906708688919291E-4 96-97 6.28373264778648E-5 98-99 2.2621437532031327E-4 100 5.0269861182291835E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3978527.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.98532194378839 #Duplication Level Percentage of deduplicated Percentage of total 1 77.28478196069543 51.769460009926725 2 15.552826613170684 20.836221956383167 3 4.185306962844523 8.410624030191588 4 1.4448026200660653 3.8712227460141744 5 0.6006246402783612 2.011651744820906 6 0.28814196497137784 1.1580769373474127 7 0.1590595577412289 0.7458258978472972 8 0.10625930312921567 0.5694250903706491 9 0.0717024878013996 0.432271280659258 >10 0.2613441504227858 3.03167962393208 >50 0.02227320368501601 1.0538438387232199 >100 0.02039822757138152 2.6945395993673897 >500 0.0016146550541306984 0.7307468809626545 >1k 7.134521218285985E-4 0.9527980338598183 >5k 3.7550111675187074E-5 0.1709653705423701 >10k+ 1.1265033502556124E-4 1.5606469590513308 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26280 0.6605459759353147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22347 0.5616902939203379 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13419 0.33728563360258706 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6797 0.1708421232280188 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4925 0.12378953316139366 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4381 0.11011613091981026 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4264 0.10717534404064619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 2.5134930591145918E-5 0.0 7 0.0 0.0 0.0 2.5134930591145918E-5 0.0 8 0.0 0.0 0.0 2.5134930591145918E-5 0.0 9 0.0 0.0 0.0 2.5134930591145918E-5 0.0 10-11 0.0 0.0 0.0 3.770239588671887E-5 0.0 12-13 0.0 0.0 0.0 6.28373264778648E-5 0.0 14-15 0.0 0.0 0.0 1.1310718766015663E-4 0.0 16-17 0.0 0.0 0.0 1.5080958354687552E-4 0.0 18-19 0.0 0.0 0.0 1.6337704884244848E-4 0.0 20-21 0.0 0.0 0.0 2.3878184061588623E-4 0.0 22-23 0.0 0.0 0.0 4.021588894583347E-4 0.0 24-25 0.0 0.0 0.0 6.032383341875021E-4 0.0 26-27 0.0 0.0 0.0 0.001256746529557296 0.0 28-29 0.0 0.0 0.0 0.0023249810796809973 0.0 30-31 0.0 0.0 0.0 0.006359137439559917 0.0 32-33 0.0 0.0 0.0 0.014213803249293017 0.0 34-35 0.0 0.0 0.0 0.0258889785088803 0.0 36-37 0.0 0.0 0.0 0.04486585110519546 0.0 38-39 0.0 0.0 0.0 0.0717602268377216 0.0 40-41 0.0 0.0 0.0 0.10095444871933759 0.0 42-43 0.0 0.0 0.0 0.12952029733617493 0.0 44-45 0.0 0.0 0.0 0.16370380294013337 0.0 46-47 0.0 0.0 0.0 0.20799155064173247 0.0 48-49 0.0 0.0 0.0 0.25560967664665846 0.0 50-51 0.0 0.0 0.0 0.30497468032766906 0.0 52-53 0.0 0.0 0.0 0.352856723103802 0.0 54-55 0.0 0.0 0.0 0.4080153282860717 0.0 56-57 0.0 0.0 0.0 0.4776767884194326 0.0 58-59 0.0 0.0 0.0 0.5570654666915671 0.0 60-61 0.0 0.0 0.0 0.6404380314623981 0.0 62-63 0.0 0.0 0.0 0.7254066643257668 0.0 64-65 0.0 0.0 0.0 0.8180917208806173 0.0 66-67 0.0 0.0 0.0 0.9316513372914146 0.0 68-69 0.0 0.0 0.0 1.059299082298549 0.0 70-71 0.0 0.0 0.0 1.200683066873745 0.0 72-73 0.0 0.0 0.0 1.3528625041378382 0.0 74-75 0.0 0.0 0.0 1.5069647635921535 0.0 76-77 0.0 0.0 0.0 1.67622338619293 0.0 78-79 0.0 0.0 0.0 1.8750406871688945 0.0 80-81 0.0 0.0 0.0 2.1005387169673604 0.0 82-83 0.0 0.0 0.0 2.3400243356397983 0.0 84-85 0.0 0.0 0.0 2.5800000854587637 0.0 86-87 0.0 0.0 0.0 2.8391914897146604 0.0 88 0.0 0.0 0.0 3.0558043215491564 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11505 0.0 59.71665 1 GTATCAA 22045 0.0 44.433548 1 TATCAAC 28420 0.0 34.217426 2 ATCAACG 28580 0.0 33.959625 3 TCAACGC 28890 0.0 33.611504 4 CAACGCA 29820 0.0 32.500183 5 AACGCAG 30945 0.0 31.331701 6 CGCAGAG 34395 0.0 27.956675 8 ACGCAGA 34785 0.0 27.73781 7 GTGGTAT 4160 0.0 26.903912 1 GTACATG 19770 0.0 26.640549 1 TACATGG 19905 0.0 26.01034 2 TGGTATC 4235 0.0 25.871607 2 GCAGAGT 37110 0.0 25.683382 9 CATGGGT 6705 0.0 25.037148 4 ACATGGG 20145 0.0 24.906494 3 GAGTACT 25175 0.0 22.952726 12-13 AGAGTAC 34845 0.0 22.281517 10-11 TATAACG 340 0.0 22.12901 2 CAGAGTA 36990 0.0 21.777184 10-11 >>END_MODULE