FastQCFastQC Report
Wed 25 May 2016
SRR2431382_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431382_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971678
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA277900.6997042559844983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58650.047.485851
GTATCAA102250.037.5897331
TCAACGC118850.031.1092874
ATCAACG119350.031.0215033
TATCAAC128800.029.3298742
CAACGCA128300.028.964215
GTACATG232950.028.51561
CATGGGT112500.028.4748424
TACATGG229850.027.6721382
AACGCAG136450.027.1395936
ACATGGG235850.026.3099163
ATGGGTG59700.025.3713045
CGCAGAG151050.024.0188798
ACGCAGA153100.023.7893527
GCAGAGT168750.021.0542419
TGGTATC29000.020.764552
ATGGGTA43900.020.6801665
GTGGTAT31550.020.5773891
GTACCGT2151.1967531E-619.672286
TACCTGG28150.019.3860842
Produced by FastQC (version 0.11.5)