##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431382_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3971678 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.661304869125846 31.0 31.0 34.0 26.0 34.0 2 30.811455258961075 33.0 31.0 34.0 27.0 34.0 3 30.896311584171727 34.0 31.0 34.0 27.0 34.0 4 34.112262625519996 37.0 35.0 37.0 30.0 37.0 5 34.19490829820544 37.0 35.0 37.0 30.0 37.0 6 34.26538707317159 37.0 35.0 37.0 31.0 37.0 7 34.17873629231776 37.0 35.0 37.0 30.0 37.0 8 34.14898035540646 37.0 35.0 37.0 30.0 37.0 9 35.57158712262172 39.0 37.0 39.0 29.0 39.0 10-11 35.597093344425204 39.0 37.0 39.0 30.0 39.0 12-13 35.52196603047881 39.0 37.0 39.0 29.5 39.0 14-15 36.871167803633625 41.0 38.0 41.0 29.5 41.0 16-17 36.897393998204286 41.0 38.0 41.0 30.0 41.0 18-19 36.924141005388655 41.0 38.0 41.0 30.0 41.0 20-21 37.00471614264802 41.0 38.0 41.0 30.0 41.0 22-23 36.982522752348004 40.5 38.0 41.0 30.0 41.0 24-25 37.02937058341588 40.5 38.0 41.0 30.0 41.0 26-27 37.0724689916957 40.0 38.0 41.0 30.0 41.0 28-29 37.03826468308861 40.0 38.0 41.0 30.0 41.0 30-31 36.96959824034073 40.0 38.0 41.0 30.0 41.0 32-33 36.91177847247435 40.0 38.0 41.0 30.0 41.0 34-35 36.83296380018723 40.0 38.0 41.0 30.0 41.0 36-37 36.701478820790605 40.0 37.0 41.0 30.0 41.0 38-39 36.57739600239496 40.0 37.0 41.0 29.0 41.0 40-41 36.51322136386686 40.0 37.0 41.0 29.0 41.0 42-43 36.35040126616508 40.0 37.0 41.0 28.0 41.0 44-45 36.199843491844 40.0 36.0 41.0 28.0 41.0 46-47 36.072567690532814 40.0 36.0 41.0 28.0 41.0 48-49 35.93895602815737 40.0 36.0 41.0 27.0 41.0 50-51 35.80847024859518 40.0 35.0 41.0 27.0 41.0 52-53 35.648585937732115 40.0 35.0 41.0 27.0 41.0 54-55 35.48641392882303 39.5 35.0 41.0 27.0 41.0 56-57 35.2961982315787 39.0 35.0 41.0 27.0 41.0 58-59 35.08225213625072 39.0 35.0 41.0 26.5 41.0 60-61 34.84872804391494 39.0 34.0 41.0 26.0 41.0 62-63 34.74819383645905 38.5 34.0 41.0 26.0 41.0 64-65 34.49743861410718 38.0 34.0 40.5 26.0 41.0 66-67 34.22768474685007 37.5 34.0 40.0 25.0 41.0 68-69 33.928479348023686 37.0 34.0 40.0 25.0 41.0 70-71 33.59320078314506 36.5 33.5 40.0 23.5 41.0 72-73 33.25138618991771 36.0 33.0 39.0 23.0 41.0 74-75 32.964324398906456 36.0 33.0 39.0 22.5 41.0 76-77 32.69764089636672 35.0 33.0 39.0 22.0 40.5 78-79 32.351765802766494 35.0 33.0 37.5 21.5 39.5 80-81 32.02896496140926 35.0 33.0 37.0 20.0 39.0 82-83 31.703350196063226 35.0 32.5 37.0 19.5 39.0 84-85 31.404104008431702 35.0 32.0 36.0 18.5 38.0 86-87 31.121920382266637 35.0 32.0 36.0 17.5 37.0 88-89 30.858482988802216 35.0 32.0 35.5 14.0 37.0 90-91 30.62641483020527 35.0 32.0 35.0 8.5 36.5 92-93 30.399606664991474 35.0 31.5 35.0 3.5 36.0 94-95 30.18688171599007 35.0 31.0 35.0 2.0 36.0 96-97 29.987549343123987 35.0 31.0 35.0 2.0 36.0 98-99 29.773896952371267 35.0 31.0 35.0 2.0 35.5 100 29.60344292764922 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34264038369222405 1101 2 0.35953253448077405 1101 3 0.3867660235830641 1101 4 0.44719731039116795 1101 5 0.3161347871017597 1101 6 0.38491756654835996 1101 7 0.3582856966728727 1101 8 0.3257145149488281 1101 9 0.32448811778042597 1101 10-11 0.3105560424110365 1101 12-13 0.2395691866912273 1101 14-15 0.32272707599704376 1101 16-17 0.2641660203462024 1101 18-19 0.20596854983003965 1101 20-21 0.25514149043897305 1101 22-23 0.33389801430186594 1101 24-25 0.32312635169392934 1101 26-27 0.33499353791250286 1101 28-29 0.4051566944484435 1101 30-31 0.3876441657158267 1101 32-33 0.38446049244817004 1101 34-35 0.34833043867396185 1101 36-37 0.3307854667563319 1101 38-39 0.4011593053773197 1101 40-41 0.34390951100113654 1101 42-43 0.3403208188950586 1101 44-45 0.30946195711442925 1101 46-47 0.3568523721145027 1101 48-49 0.41016749539461017 1101 50-51 0.35386071206804814 1101 52-53 0.3363848652441561 1101 54-55 0.3400638355445693 1101 56-57 0.25714441648666053 1101 58-59 0.26067042144075714 1101 60-61 0.2303382193712622 1101 62-63 0.23881146507557816 1101 64-65 0.25918550080329794 1101 66-67 0.26623138539643776 1101 68-69 0.25935045960221714 1101 70-71 0.23554954665966932 1101 72-73 0.17515169681046672 1101 74-75 0.22502858779310486 1101 76-77 0.23182869286546293 1101 78-79 0.17279515263451373 1101 80-81 0.18895450110706236 1101 82-83 0.20889204255562888 1101 84-85 0.15496667275522213 1101 86-87 0.19995762806625095 1101 88-89 0.16450898214866783 1101 90-91 0.15203937048111626 1101 92-93 0.14195132801487986 1101 94-95 0.1360769467955727 1101 96-97 0.12630556260959125 1101 98-99 0.06575949831510286 1101 100 0.15557757241772663 1102 1 0.056445340416459544 1102 2 0.1463455129628244 1102 3 0.21921460237894053 1102 4 0.23057593086370076 1102 5 0.22488847878441476 1102 6 0.14364071283644364 1102 7 0.05107460988147494 1102 8 0.11145259537379104 1102 9 0.18100162646556583 1102 10-11 0.16859803889300906 1102 12-13 0.1333223099422014 1102 14-15 0.16501258361277849 1102 16-17 0.1481089029569418 1102 18-19 0.07114063158925887 1102 20-21 0.11736272467280173 1102 22-23 0.0756214148225709 1102 24-25 0.10707277919452451 1102 26-27 0.08119302557440733 1102 28-29 0.0829537218630989 1102 30-31 0.13199062857162858 1102 32-33 0.1019398914847045 1102 34-35 0.08975133672105784 1102 36-37 0.04669819625281235 1102 38-39 0.0901884178243435 1102 40-41 0.07367601363466036 1102 42-43 0.07609074086376211 1102 44-45 -0.0432043778629847 1102 46-47 -0.10798541247612548 1102 48-49 -0.09179966861854894 1102 50-51 -0.12173470449867096 1102 52-53 -0.16747773732340931 1102 54-55 -0.22646750065715793 1102 56-57 -0.22675514663298912 1102 58-59 -0.15406349943855702 1102 60-61 -0.19607339637391163 1102 62-63 -0.1773859799801798 1102 64-65 -0.15462431257620324 1102 66-67 -0.2193066195707729 1102 68-69 -0.20819216965039544 1102 70-71 -0.25395762619853457 1102 72-73 -0.22869753824290484 1102 74-75 -0.21020549362130225 1102 76-77 -0.23232744219890122 1102 78-79 -0.24710798285961033 1102 80-81 -0.23792525872743298 1102 82-83 -0.25062113006770304 1102 84-85 -0.19561445493826923 1102 86-87 -0.24240071376232208 1102 88-89 -0.2722123913821548 1102 90-91 -0.23551080454586426 1102 92-93 -0.20531790434000285 1102 94-95 -0.2557204747733479 1102 96-97 -0.19099079830329657 1102 98-99 -0.26791776741608686 1102 100 -0.3007943905330208 1103 1 -0.2606354531708419 1103 2 -0.18050665149406697 1103 3 -0.17493385946156792 1103 4 -0.07588606377117912 1103 5 -0.1683110874557059 1103 6 -0.09273768577494224 1103 7 -0.07243701637555233 1103 8 -0.1170502052275566 1103 9 -0.15535313308883758 1103 10-11 -0.15367379069049036 1103 12-13 -0.12093091603983908 1103 14-15 -0.1725586546579052 1103 16-17 -0.17931275482290232 1103 18-19 -0.23254703071901872 1103 20-21 -0.356590520758715 1103 22-23 -0.23834526175231474 1103 24-25 -0.16026825808607015 1103 26-27 -0.22976395256512205 1103 28-29 -0.2761219023242134 1103 30-31 -0.29288481531054344 1103 32-33 -0.23828288139808507 1103 34-35 -0.2607953891013608 1103 36-37 -0.2653101847561814 1103 38-39 -0.214465250113264 1103 40-41 -0.27138030420147885 1103 42-43 -0.33163826747015435 1103 44-45 -0.3983885484409555 1103 46-47 -0.4818660282047489 1103 48-49 -0.5173244984968406 1103 50-51 -0.5866757811675072 1103 52-53 -0.5928179960135935 1103 54-55 -0.5130329628022423 1103 56-57 -0.5000221866316181 1103 58-59 -0.5219645958259704 1103 60-61 -0.44088853772507974 1103 62-63 -0.45536840022818836 1103 64-65 -0.43954293061186434 1103 66-67 -0.4658635282304431 1103 68-69 -0.44129207876387966 1103 70-71 -0.48572637811749075 1103 72-73 -0.4627443857774054 1103 74-75 -0.42338965013204444 1103 76-77 -0.43269263963548354 1103 78-79 -0.39256822887705667 1103 80-81 -0.382083537045677 1103 82-83 -0.4408700955149243 1103 84-85 -0.44376664826355494 1103 86-87 -0.4721658033093945 1103 88-89 -0.5190541460913103 1103 90-91 -0.4730187861679518 1103 92-93 -0.4493280639357451 1103 94-95 -0.47443982503872917 1103 96-97 -0.4395323327074543 1103 98-99 -0.49980959227789157 1103 100 -0.5095198936810199 1104 1 -0.32252406304530723 1104 2 -0.2643195450765532 1104 3 -0.17364323947235505 1104 4 -0.18762201758518415 1104 5 -0.2245221378800366 1104 6 -0.05902941856592747 1104 7 -0.18318709926051469 1104 8 -0.17637915911880953 1104 9 -0.18429533326867897 1104 10-11 -0.21610141013989193 1104 12-13 -0.19797248953912572 1104 14-15 -0.29126687464247425 1104 16-17 -0.20752093266872862 1104 18-19 -0.27690686334587866 1104 20-21 -0.366980020753509 1104 22-23 -0.29759947387049834 1104 24-25 -0.3569710470167564 1104 26-27 -0.37226289667486157 1104 28-29 -0.32576545460059236 1104 30-31 -0.3181037006343175 1104 32-33 -0.38313151432292614 1104 34-35 -0.34259588541821273 1104 36-37 -0.44473456170222647 1104 38-39 -0.407682677896787 1104 40-41 -0.345603546767812 1104 42-43 -0.4506073571304938 1104 44-45 -0.4814838725665638 1104 46-47 -0.5627689269867702 1104 48-49 -0.5492050281469005 1104 50-51 -0.5962339189451953 1104 52-53 -0.6918001850097113 1104 54-55 -0.5437228308813502 1104 56-57 -0.5079118482793348 1104 58-59 -0.6301010173315476 1104 60-61 -0.6587775012985659 1104 62-63 -0.5433717825885722 1104 64-65 -0.5730958838715239 1104 66-67 -0.5498136603014814 1104 68-69 -0.5323788090083781 1104 70-71 -0.6429692040576072 1104 72-73 -0.6754276862675468 1104 74-75 -0.6684132678058035 1104 76-77 -0.6456834439453587 1104 78-79 -0.5868879237489892 1104 80-81 -0.624068306797426 1104 82-83 -0.6300813915063905 1104 84-85 -0.5812157245654284 1104 86-87 -0.5159922115606435 1104 88-89 -0.5558707810610883 1104 90-91 -0.6137336450625099 1104 92-93 -0.6101226982453518 1104 94-95 -0.6499805207686968 1104 96-97 -0.6836734737518668 1104 98-99 -0.6712428355537945 1104 100 -0.5835426745133212 1105 1 -0.09597679417220206 1105 2 -0.08187557555115177 1105 3 -0.0780050936948129 1105 4 0.020049028402034708 1105 5 0.017431741625749453 1105 6 0.021547675354739226 1105 7 -0.07648841129240935 1105 8 -0.06864905774683194 1105 9 -0.1043242829365596 1105 10-11 -0.11784748669766998 1105 12-13 -0.08881816928742126 1105 14-15 -0.14660538842694848 1105 16-17 -0.14595902075745215 1105 18-19 -0.1411402561831423 1105 20-21 -0.10079740549974758 1105 22-23 -0.23935499797410387 1105 24-25 -0.20789257745458656 1105 26-27 -0.13405399096495074 1105 28-29 -0.15864127242078752 1105 30-31 -0.16488519412583003 1105 32-33 -0.12208724682633942 1105 34-35 -0.20415414318598124 1105 36-37 -0.22960258896022623 1105 38-39 -0.23188685732193193 1105 40-41 -0.22598083798232693 1105 42-43 -0.2587471800911345 1105 44-45 -0.29637225866684247 1105 46-47 -0.2928569323384451 1105 48-49 -0.43229040750904346 1105 50-51 -0.3166477438200417 1105 52-53 -0.3259970654177593 1105 54-55 -0.4258807545312209 1105 56-57 -0.4343719260587662 1105 58-59 -0.3509578547767944 1105 60-61 -0.43846751450341515 1105 62-63 -0.2690355979766892 1105 64-65 -0.3002654037454704 1105 66-67 -0.2432516977046646 1105 68-69 -0.2383251560744455 1105 70-71 -0.2091558053779039 1105 72-73 -0.24888452307286713 1105 74-75 -0.2887680313152856 1105 76-77 -0.3233152772647401 1105 78-79 -0.4295301129170639 1105 80-81 -0.3651866373837187 1105 82-83 -0.3004981304261243 1105 84-85 -0.292312201160712 1105 86-87 -0.28871990021111316 1105 88-89 -0.2570167587416883 1105 90-91 -0.25463372579755017 1105 92-93 -0.309108350469149 1105 94-95 -0.2674589769462905 1105 96-97 -0.26911077291040897 1105 98-99 -0.33209498839902096 1105 100 -0.2979448950603789 1106 1 -0.21664815385718228 1106 2 -0.12166960237693303 1106 3 -0.18221866021140087 1106 4 -0.06086222518195683 1106 5 -0.05854869857017775 1106 6 -0.02632718394571043 1106 7 -0.05265797921645543 1106 8 -0.08242685115789072 1106 9 -0.07284544200218335 1106 10-11 -0.07030333258896349 1106 12-13 -0.10261368139177307 1106 14-15 -0.1414913652084948 1106 16-17 -0.05732375855696148 1106 18-19 -0.1070062216620542 1106 20-21 -0.1466116901440344 1106 22-23 -0.11415889651829048 1106 24-25 -0.11770862854322672 1106 26-27 -0.19346573209737983 1106 28-29 -0.10309583766617436 1106 30-31 -0.0908135603217275 1106 32-33 -0.14099961130677485 1106 34-35 -0.06885860529837373 1106 36-37 -0.09843498161323083 1106 38-39 -0.1809491432392818 1106 40-41 -0.1888883212617074 1106 42-43 -0.11669885131244939 1106 44-45 -0.17786588997055475 1106 46-47 -0.19445382847838033 1106 48-49 -0.22275694259946732 1106 50-51 -0.2063001698489444 1106 52-53 -0.23823917749299994 1106 54-55 -0.2584801985463372 1106 56-57 -0.2502164716062225 1106 58-59 -0.28141440439916465 1106 60-61 -0.26635767983822234 1106 62-63 -0.2695221255864695 1106 64-65 -0.18183861604453 1106 66-67 -0.10961007952450075 1106 68-69 -0.12290235042742381 1106 70-71 -0.24420511613678286 1106 72-73 -0.20334926045237722 1106 74-75 -0.2745236602306065 1106 76-77 -0.29285942472347415 1106 78-79 -0.28060776238525165 1106 80-81 -0.277440941086617 1106 82-83 -0.26248271040880056 1106 84-85 -0.23756899300564882 1106 86-87 -0.25014118616299896 1106 88-89 -0.2706287401833478 1106 90-91 -0.2694303367817277 1106 92-93 -0.2217120551372922 1106 94-95 -0.21064134001272805 1106 96-97 -0.2422676525583931 1106 98-99 -0.2197750249062409 1106 100 -0.24956848736488269 1107 1 -0.3279328933129868 1107 2 -0.3224475894852965 1107 3 -0.30662743343767573 1107 4 -0.21395035105972227 1107 5 -0.15549506858025097 1107 6 -0.2869507972167611 1107 7 -0.3043352089203495 1107 8 -0.21512196173775067 1107 9 -0.24223093610508073 1107 10-11 -0.21128671930044618 1107 12-13 -0.16769396518522228 1107 14-15 -0.25474384948687145 1107 16-17 -0.25563366685591404 1107 18-19 -0.33429794634844257 1107 20-21 -0.27454210289063496 1107 22-23 -0.2875436876059183 1107 24-25 -0.3099681132177494 1107 26-27 -0.2849756105018173 1107 28-29 -0.3059512757116778 1107 30-31 -0.3786006315961501 1107 32-33 -0.32271309127721537 1107 34-35 -0.33036676091900574 1107 36-37 -0.3474567603809078 1107 38-39 -0.37892815239100486 1107 40-41 -0.31044644450618364 1107 42-43 -0.36050016143237684 1107 44-45 -0.49322952258457775 1107 46-47 -0.4600744769987699 1107 48-49 -0.41491416032257433 1107 50-51 -0.5037572771852084 1107 52-53 -0.45984904744149446 1107 54-55 -0.4230606251487359 1107 56-57 -0.4956445888396175 1107 58-59 -0.583515933523465 1107 60-61 -0.585999175938376 1107 62-63 -0.5435554192595831 1107 64-65 -0.4331233221820696 1107 66-67 -0.36988436807625646 1107 68-69 -0.45617668746298534 1107 70-71 -0.47026452477673786 1107 72-73 -0.4551834306484821 1107 74-75 -0.41077201959354426 1107 76-77 -0.41587101396309833 1107 78-79 -0.3823279065820806 1107 80-81 -0.3641440860775731 1107 82-83 -0.38737936635847703 1107 84-85 -0.39216514352881404 1107 86-87 -0.35028923987111327 1107 88-89 -0.35666228868370453 1107 90-91 -0.39650761538304025 1107 92-93 -0.34710664673355396 1107 94-95 -0.45667360920680267 1107 96-97 -0.5384008473567015 1107 98-99 -0.5524878774593809 1107 100 -0.5708881466546565 1108 1 -0.4076315906026018 1108 2 -0.4036265691826486 1108 3 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0.38609903313149374 2213 96-97 0.37573261707189687 2213 98-99 0.3609796110275951 2213 100 0.39589682823812566 2214 1 0.26391081432291585 2214 2 0.16971130765335474 2214 3 0.16283600313181879 2214 4 0.09799113625869182 2214 5 0.1500122672277655 2214 6 0.08714416512667356 2214 7 0.07028080651586066 2214 8 0.13931804732447262 2214 9 0.16226875547999242 2214 10-11 0.14276758087257235 2214 12-13 0.05244955514352512 2214 14-15 0.04342437692724843 2214 16-17 0.18172587425998188 2214 18-19 0.14855592633914227 2214 20-21 0.13215210880056105 2214 22-23 0.19495449540919907 2214 24-25 0.2383088254701562 2214 26-27 0.19453364771425896 2214 28-29 0.169945244636466 2214 30-31 0.16540227508876626 2214 32-33 0.18361141522821356 2214 34-35 0.19268670887780814 2214 36-37 0.18554638611409757 2214 38-39 0.23163326096182857 2214 40-41 0.18983603804131377 2214 42-43 0.20356622251856038 2214 44-45 0.2621390449145977 2214 46-47 0.2935193239716085 2214 48-49 0.3154167604279152 2214 50-51 0.3360388523240161 2214 52-53 0.2626321181386686 2214 54-55 0.34521854963944065 2214 56-57 0.41280883976974536 2214 58-59 0.3409891114476764 2214 60-61 0.41625917488980235 2214 62-63 0.34893892973945384 2214 64-65 0.3471183631944399 2214 66-67 0.29585945926832835 2214 68-69 0.27576843505248405 2214 70-71 0.3005312446491786 2214 72-73 0.3165483345182807 2214 74-75 0.3410303484145629 2214 76-77 0.4106978337481664 2214 78-79 0.41105171476342406 2214 80-81 0.38322351334482363 2214 82-83 0.45907923743621737 2214 84-85 0.43392423126393 2214 86-87 0.436251890996715 2214 88-89 0.39615903904931216 2214 90-91 0.45044911595380555 2214 92-93 0.4535150185990595 2214 94-95 0.45641302654718885 2214 96-97 0.45259358399268734 2214 98-99 0.4826414665826846 2214 100 0.5107736219460293 2215 1 0.16376915010654258 2215 2 0.07642534965649972 2215 3 0.05799348989051367 2215 4 0.03630653560650643 2215 5 0.11100601728778514 2215 6 0.12658329497777032 2215 7 0.09841360757479833 2215 8 0.029211501857368205 2215 9 -0.052743278724570075 2215 10-11 -0.03867579624868256 2215 12-13 0.0035332567704529083 2215 14-15 0.037704712287087716 2215 16-17 0.09834633778169888 2215 18-19 0.15779287051960011 2215 20-21 0.10432552149418939 2215 22-23 0.15110936935910502 2215 24-25 0.22199935709817709 2215 26-27 0.10866597635480701 2215 28-29 0.11852427060864557 2215 30-31 0.145186905839239 2215 32-33 0.1541109302433341 2215 34-35 0.17464844870972485 2215 36-37 0.047118051799621696 2215 38-39 0.11423821109737275 2215 40-41 0.09393912046912334 2215 42-43 0.1801023127700887 2215 44-45 0.18899110582960077 2215 46-47 0.23053788171831968 2215 48-49 0.2492597348492822 2215 50-51 0.19181550549720328 2215 52-53 0.3183668727865836 2215 54-55 0.3363003959207873 2215 56-57 0.2879195526127276 2215 58-59 0.24497441621168292 2215 60-61 0.2764197256250256 2215 62-63 0.29068611561137203 2215 64-65 0.18718316134285828 2215 66-67 0.19105290867246083 2215 68-69 0.21127389514242623 2215 70-71 0.19838264005090167 2215 72-73 0.23671285544782705 2215 74-75 0.25358611350247884 2215 76-77 0.2488044045525868 2215 78-79 0.28753232854769806 2215 80-81 0.2937770172813998 2215 82-83 0.3090677845596481 2215 84-85 0.27734385383188 2215 86-87 0.24803895453842273 2215 88-89 0.2509514804368429 2215 90-91 0.35433056836245314 2215 92-93 0.3632985745568327 2215 94-95 0.3411767570749973 2215 96-97 0.36250086371075696 2215 98-99 0.36269839177241536 2215 100 0.41527610428806483 2216 1 0.3249340432699057 2216 2 0.13745386764004053 2216 3 0.2655259823615772 2216 4 0.12958472343477467 2216 5 0.21850080090233348 2216 6 0.14035631216588484 2216 7 0.1509947424478213 2216 8 0.1357030378994608 2216 9 0.0951019661435808 2216 10-11 0.11712968345753438 2216 12-13 0.10483617515043875 2216 14-15 0.21817718313285894 2216 16-17 0.18700287670333893 2216 18-19 0.27376527646888604 2216 20-21 0.22220859983995922 2216 22-23 0.2717842370865924 2216 24-25 0.2612700041857039 2216 26-27 0.24341218853652435 2216 28-29 0.2311566688390556 2216 30-31 0.2328497489282313 2216 32-33 0.23900027634344667 2216 34-35 0.2687620463966667 2216 36-37 0.27669830713012544 2216 38-39 0.3150482921466988 2216 40-41 0.30429986305897927 2216 42-43 0.3540011541392758 2216 44-45 0.41500631979496205 2216 46-47 0.47368184613233666 2216 48-49 0.47732189333522257 2216 50-51 0.5511117272010821 2216 52-53 0.5432190264539045 2216 54-55 0.5166517571944809 2216 56-57 0.5562831636253449 2216 58-59 0.549362752661402 2216 60-61 0.5408115877259192 2216 62-63 0.47619916646549854 2216 64-65 0.4745480454945721 2216 66-67 0.42274843065843015 2216 68-69 0.35971893392582643 2216 70-71 0.3712844171916885 2216 72-73 0.3781547513557726 2216 74-75 0.38697114073714545 2216 76-77 0.40875235262799947 2216 78-79 0.39912310048686805 2216 80-81 0.38335281335211846 2216 82-83 0.33789827507192527 2216 84-85 0.33843664805852924 2216 86-87 0.31323290896079925 2216 88-89 0.355980025825577 2216 90-91 0.4131564322448007 2216 92-93 0.40414783510617625 2216 94-95 0.3583377315249834 2216 96-97 0.3998661152370744 2216 98-99 0.4094400833952676 2216 100 0.42687926390816955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97038.0 3 20584.0 4 14728.0 5 12568.0 6 8457.0 7 6992.0 8 8006.0 9 8040.0 10 8278.0 11 8764.0 12 10279.0 13 11838.0 14 12861.0 15 13631.0 16 13808.0 17 13387.0 18 12692.0 19 11416.0 20 10958.0 21 11432.0 22 13610.0 23 20643.0 24 33580.0 25 43056.0 26 46676.0 27 35896.0 28 35338.0 29 43538.0 30 51057.0 31 58580.0 32 74804.0 33 99950.0 34 141541.0 35 226370.0 36 377037.0 37 686119.0 38 1079810.0 39 585884.0 40 2432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99591342515514 22.027924928106554 13.940189697795269 22.03597194894304 2 14.577057088508516 23.061594161771165 40.28402617022248 22.077322579497842 3 17.192836574581676 29.652288383728553 30.592370658272035 22.56250438341774 4 11.465629321465851 19.56991813273828 39.38424740087715 29.580205144918725 5 11.675713451347304 41.19866638241724 34.483097040484104 12.64252312575136 6 26.908941365669104 40.932400944148625 19.12268867160244 13.035969018579832 7 24.292753853267765 36.083671317173106 23.338108575654832 16.285466253904296 8 23.589743072900617 38.05446841404944 21.180593262211474 17.175195250838463 9 24.934109697999318 19.080914374226683 21.56601362980043 34.41896229797357 10-11 21.93885804652963 29.998692223492203 29.21318218561456 18.84926754436361 12-13 22.782090671475093 28.323301645344657 28.66829814410202 20.226309539078226 14-15 20.729520082747392 29.677879812180546 26.282162569402363 23.310437535669696 16-17 19.84157959318395 32.626137918349556 27.063320290158156 20.468962198308336 18-19 19.996512689564234 31.47550901440771 28.793820203697734 19.734158092330322 20-21 21.879323764765527 30.382558769183944 27.962963942872822 19.77515352317771 22-23 21.36650787773433 30.464658890204326 28.038855686599508 20.12997754546184 24-25 20.93336755104846 30.541402112452566 28.08357235969571 20.441657976803263 26-27 20.610634126256784 30.546384169786396 28.190380245947967 20.652601458008856 28-29 20.534980408955185 30.528177087926622 28.32385067970217 20.612991823416024 30-31 20.77072594105278 30.84056617573593 28.00608404187617 20.382623841335125 32-33 20.490704684169916 30.67914088694181 28.293551461261245 20.536602967627033 34-35 20.795047583290444 30.387967446692038 28.26318312590122 20.553801844116297 36-37 20.89290788229387 30.526542598130966 27.945762489754234 20.634787029820927 38-39 20.70698511748877 30.533925540950563 28.197052376505848 20.56203696505482 40-41 20.622288264286297 30.278936866678958 28.360133243812015 20.738641625222737 42-43 20.577778712950316 30.385696093648512 28.329033289608418 20.70749190379275 44-45 20.714652943340877 30.395457088564847 28.270399754028542 20.619490214065735 46-47 20.763929496651066 30.493400217546306 28.128626743025848 20.614043542776777 48-49 20.76712649819058 30.326439220588085 28.13327217926877 20.77316210195256 50-51 20.65751645466938 30.366454940281617 28.114908572430092 20.861120032618906 52-53 20.73637511371397 30.353640988939823 28.133931743899765 20.776052153446447 54-55 20.809870983008324 30.498065009725984 28.054297160174656 20.637766847091036 56-57 20.75647231403204 30.321787869697353 27.92641148121601 20.995328335054598 58-59 20.764013587604683 30.11399067405159 28.207169768575003 20.914825969768728 60-61 20.899062540988552 30.491212495119175 27.857134487310603 20.752590476581666 62-63 20.712705483939246 30.389663565719584 28.030440851870143 20.867190098471028 64-65 20.811360672161847 30.22452935164523 28.087977829786464 20.876132146406462 66-67 20.643417583918392 30.361342835357323 27.94279433486924 21.052445245855044 68-69 20.966381663293525 30.509425471097167 27.76680135705013 20.757391508559174 70-71 20.873180710791125 30.254090101539788 27.916752440102005 20.955976747567078 72-73 20.815405334243746 30.422977738080093 28.03245747861175 20.72915944906441 74-75 20.83115113409057 30.471950954785925 27.92422992671716 20.77266798440634 76-77 20.88317093249721 30.397057004041606 27.778001765414555 20.94177029804663 78-79 20.961848708898525 30.27982737017959 28.047344062117507 20.71097985880438 80-81 20.857573486534967 30.292005751613164 28.00432580580292 20.846094956048947 82-83 20.806980247596783 30.26865153345601 28.166490517425167 20.75787770152204 84-85 20.735241270060477 30.226862755870147 27.987001924838072 21.0508940492313 86-87 20.965151844466913 30.25650233255529 27.847251490724904 20.931094332252894 88-89 20.840823579056234 30.089852708389277 28.02169438083834 21.04762933171615 90-91 20.76778091136656 30.105673887276733 27.95627389959941 21.170271301757293 92-93 20.82003646062341 30.20408626074511 27.93286911341723 21.043008165214246 94-95 20.785418010261104 30.123934263727463 28.0781766919022 21.012471034109232 96-97 20.781105982655305 30.254697626883924 27.90423377292246 21.05996261753831 98-99 20.663832553826307 30.26801014031126 27.902701899040398 21.16545540682203 100 20.69068410640255 30.415655439206645 27.761110901095027 21.13254955329578 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 26096.0 1 19481.5 2 11413.5 3 8239.0 4 5510.0 5 3930.0 6 3157.0 7 3069.5 8 3330.5 9 3331.0 10 2653.0 11 1978.5 12 1855.5 13 1852.5 14 1886.5 15 2053.0 16 2337.0 17 2834.5 18 3579.0 19 4565.0 20 6128.5 21 8752.5 22 13140.0 23 19831.0 24 26951.0 25 34061.0 26 44269.5 27 56278.5 28 71591.5 29 86757.5 30 95250.5 31 103243.5 32 112279.5 33 120676.0 34 128263.5 35 133258.5 36 134956.0 37 135720.5 38 135328.0 39 133084.5 40 131954.0 41 128984.5 42 123137.0 43 119015.5 44 116686.0 45 114208.0 46 112313.0 47 109507.0 48 106284.5 49 105242.0 50 104298.0 51 99229.5 52 92313.0 53 87397.5 54 84312.5 55 82522.5 56 76738.0 57 68683.0 58 63269.5 59 57803.5 60 51766.5 61 44873.0 62 37922.0 63 31077.0 64 24261.5 65 19611.0 66 16182.0 67 12723.5 68 9774.0 69 7518.5 70 5854.5 71 4726.5 72 3938.0 73 3349.0 74 3108.5 75 2646.0 76 1837.0 77 1512.5 78 1317.0 79 1023.5 80 722.0 81 442.0 82 322.0 83 243.5 84 179.5 85 157.5 86 124.0 87 96.0 88 87.0 89 68.0 90 53.5 91 52.5 92 52.0 93 37.5 94 26.5 95 23.5 96 23.0 97 24.5 98 22.0 99 23.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18828313876401864 2 0.18443086272351383 3 0.19830409212428599 4 0.18772921671897871 5 0.20200529851614354 6 0.08145171889564058 7 0.08291205883256396 8 0.07072577384168606 9 0.06498512719309069 10-11 0.07830443454882294 12-13 0.0777253342290085 14-15 0.0748550109047108 16-17 0.07070059556691152 18-19 0.07562294828533431 20-21 0.07702034253532133 22-23 0.07534598726281436 24-25 0.07486760004209807 26-27 0.07843032592269565 28-29 0.07623981601731056 30-31 0.08216929972671501 32-33 0.07855621729656835 34-35 0.07831702368621021 36-37 0.09002492145637185 38-39 0.08466194892939458 40-41 0.07965147224926089 42-43 0.08327714381679481 44-45 0.09411639110723477 46-47 0.08378070931228564 48-49 0.08958430164781737 50-51 0.08629853678973974 52-53 0.08530399493614538 54-55 0.08663844349919607 56-57 0.080029146370879 58-59 0.08818690739783033 60-61 0.08420873998345284 62-63 0.07548446777407433 64-65 0.07830443454882294 66-67 0.07855621729656835 68-69 0.0772973035578413 70-71 0.07274003582364935 72-73 0.0703229214452934 74-75 0.07587473103307972 76-77 0.08069637065240436 78-79 0.07730989269522857 80-81 0.08560613423343988 82-83 0.086915404521716 84-85 0.08943323199917012 86-87 0.08536694062308173 88-89 0.08715459813207416 90-91 0.08521587097443448 92-93 0.07586214189569244 94-95 0.07557259173578523 96-97 0.07482983262993627 98-99 0.08454864669290914 100 0.09386460835948936 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3971678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.64699442266511 #Duplication Level Percentage of deduplicated Percentage of total 1 75.45803644809816 45.76303115613069 2 14.86661320374543 18.032308161029373 3 4.750375086860268 8.64287914195146 4 1.8999833251091967 4.6091311248421665 5 0.96254869228893 2.918784258639517 6 0.5448571508031037 1.9826369151543022 7 0.3283053029683286 1.3937510914636124 8 0.23015049076892352 1.1166348424029235 9 0.15293436055200368 0.83475083802881 >10 0.7294509753565078 7.711764175928557 >50 0.04501277577869686 1.89399137291959 >100 0.029945656670859817 3.3939861735400387 >500 0.00128796425368489 0.555521519466896 >1k 4.570195170256927E-4 0.4506005537910873 >5k 0.0 0.0 >10k+ 4.1547228820516416E-5 0.7002286747108485 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27790 0.6997042559844983 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.5178274774541138E-5 0.0 5 0.0 0.0 0.0 2.5178274774541138E-5 0.0 6 0.0 0.0 0.0 2.5178274774541138E-5 0.0 7 0.0 0.0 0.0 5.0356549549082276E-5 0.0 8 0.0 0.0 0.0 5.0356549549082276E-5 0.0 9 0.0 0.0 0.0 5.0356549549082276E-5 0.0 10-11 0.0 0.0 0.0 5.0356549549082276E-5 0.0 12-13 0.0 0.0 0.0 5.0356549549082276E-5 0.0 14-15 0.0 0.0 0.0 5.0356549549082276E-5 0.0 16-17 0.0 0.0 0.0 6.294568693635284E-5 0.0 18-19 0.0 0.0 0.0 7.553482432362341E-5 0.0 20-21 0.0 0.0 0.0 1.6365878603451738E-4 0.0 22-23 0.0 0.0 0.0 3.399067094563054E-4 0.0 24-25 0.0 0.0 0.0 7.679373806235047E-4 0.0 26-27 2.5178274774541138E-5 0.0 0.0 0.0014351616621488448 0.0 28-29 2.5178274774541138E-5 1.2589137387270569E-5 0.0 0.002807377637361337 0.0 30-31 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.006596707990929778 0.0 32-33 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.014339027484101178 0.0 34-35 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.026097281803811892 0.0 36-37 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.045245359769850425 0.0 38-39 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.07081389780339695 0.0 40-41 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.09946677449682476 0.0 42-43 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.12669707866549101 0.0 44-45 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.16038561031382706 0.0 46-47 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.2033145688044197 0.0 48-49 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.25174498033324955 0.0 50-51 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.3030708934611517 0.0 52-53 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.3521811184088942 0.0 54-55 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.40591155677776497 0.0 56-57 2.5178274774541138E-5 5.0356549549082276E-5 0.0 0.4724577370068772 2.5178274774541138E-5 58-59 2.5178274774541138E-5 5.0356549549082276E-5 0.0 0.5509132412043474 2.5178274774541138E-5 60-61 2.5178274774541138E-5 5.0356549549082276E-5 2.5178274774541138E-5 0.6346561831044712 2.5178274774541138E-5 62-63 2.5178274774541138E-5 5.0356549549082276E-5 2.5178274774541138E-5 0.7195573256442239 2.5178274774541138E-5 64-65 2.5178274774541138E-5 5.0356549549082276E-5 2.5178274774541138E-5 0.8092171621163649 2.5178274774541138E-5 66-67 2.5178274774541138E-5 5.0356549549082276E-5 2.5178274774541138E-5 0.914235746200976 2.5178274774541138E-5 68-69 2.5178274774541138E-5 5.0356549549082276E-5 2.5178274774541138E-5 1.0380750906795566 2.5178274774541138E-5 70-71 2.5178274774541138E-5 6.294568693635284E-5 2.5178274774541138E-5 1.1758002536962966 2.5178274774541138E-5 72-73 2.5178274774541138E-5 7.553482432362341E-5 2.5178274774541138E-5 1.319681504895412 2.5178274774541138E-5 74-75 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 1.4701720532228444 2.5178274774541138E-5 76-77 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 1.635656264178516 2.5178274774541138E-5 78-79 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 1.8284337249897902 2.5178274774541138E-5 80-81 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 2.0462761583391202 2.5178274774541138E-5 82-83 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 2.270866369328027 2.5178274774541138E-5 84-85 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 2.504734774571353 2.5178274774541138E-5 86-87 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 2.753080687810039 2.5178274774541138E-5 88 5.0356549549082276E-5 7.553482432362341E-5 2.5178274774541138E-5 2.956030171630228 2.5178274774541138E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5865 0.0 47.48585 1 GTATCAA 10225 0.0 37.589733 1 TCAACGC 11885 0.0 31.109287 4 ATCAACG 11935 0.0 31.021503 3 TATCAAC 12880 0.0 29.329874 2 CAACGCA 12830 0.0 28.96421 5 GTACATG 23295 0.0 28.5156 1 CATGGGT 11250 0.0 28.474842 4 TACATGG 22985 0.0 27.672138 2 AACGCAG 13645 0.0 27.139593 6 ACATGGG 23585 0.0 26.309916 3 ATGGGTG 5970 0.0 25.371304 5 CGCAGAG 15105 0.0 24.018879 8 ACGCAGA 15310 0.0 23.789352 7 GCAGAGT 16875 0.0 21.054241 9 TGGTATC 2900 0.0 20.76455 2 ATGGGTA 4390 0.0 20.680166 5 GTGGTAT 3155 0.0 20.577389 1 GTACCGT 215 1.1967531E-6 19.67228 6 TACCTGG 2815 0.0 19.386084 2 >>END_MODULE