FastQCFastQC Report
Wed 25 May 2016
SRR2431382_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431382_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3971678
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340910.858352565338882No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269220.6778495134801965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187180.47128694722986103No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81840.20605900075484468No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58910.14832521669682186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54040.1360633968816203No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53550.1348296614176678No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52710.13271468633660635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117100.062.9336471
GTATCAA236450.045.7466551
ATCAACG306650.035.0242843
TATCAAC309050.034.8614352
TCAACGC309550.034.726544
CAACGCA319350.033.646155
AACGCAG329000.032.7836156
GTGGTAT39350.032.1498071
TGGTATC40400.031.0798452
CGCAGAG372400.028.7736638
ACGCAGA373300.028.7546527
GCAGAGT398500.026.7593889
CATGGGT107250.026.3507674
GTACATG221000.026.1109751
TACATGG219950.025.7211632
ACATGGG221100.024.6496523
ATGGGTG54900.023.2976785
GAGTACT270800.023.1340412-13
CAGAGTA395050.022.4129410-11
AGAGTAC379800.022.21776810-11
Produced by FastQC (version 0.11.5)