##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431382_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3971678 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65800651513038 34.0 31.0 34.0 31.0 34.0 2 32.77967247093042 34.0 31.0 34.0 31.0 34.0 3 32.863747010709325 34.0 31.0 34.0 31.0 34.0 4 36.329851513642346 37.0 37.0 37.0 35.0 37.0 5 36.23773956498991 37.0 37.0 37.0 35.0 37.0 6 36.280192654087266 37.0 37.0 37.0 35.0 37.0 7 36.236544352286366 37.0 37.0 37.0 35.0 37.0 8 36.253017742123106 37.0 37.0 37.0 35.0 37.0 9 38.05144324388835 39.0 39.0 39.0 35.0 39.0 10-11 38.0282906872108 39.0 39.0 39.0 35.0 39.0 12-13 38.010989309808096 39.0 39.0 39.0 35.0 39.0 14-15 39.47561333018437 41.0 39.0 41.0 36.0 41.0 16-17 39.50487199616887 41.0 39.0 41.0 36.5 41.0 18-19 39.57814543374362 41.0 40.0 41.0 37.0 41.0 20-21 39.570843608167635 41.0 40.0 41.0 37.0 41.0 22-23 39.50008774628759 41.0 39.5 41.0 36.0 41.0 24-25 39.44655546094119 41.0 39.0 41.0 36.0 41.0 26-27 39.32337943811155 41.0 39.0 41.0 36.0 41.0 28-29 39.238425295303394 41.0 39.0 41.0 35.0 41.0 30-31 39.04993481344661 41.0 39.0 41.0 35.0 41.0 32-33 38.80869055849946 41.0 38.0 41.0 35.0 41.0 34-35 38.66291602692867 41.0 38.0 41.0 35.0 41.0 36-37 38.52018479342988 41.0 38.0 41.0 35.0 41.0 38-39 38.37009080293015 41.0 38.0 41.0 35.0 41.0 40-41 38.226805143820826 40.0 38.0 41.0 34.0 41.0 42-43 38.046377752677834 40.0 38.0 41.0 34.0 41.0 44-45 37.79051121465537 40.0 38.0 41.0 33.0 41.0 46-47 37.40427962689825 40.0 38.0 41.0 33.0 41.0 48-49 37.05437248437562 40.0 37.0 41.0 31.5 41.0 50-51 36.88934689569497 40.0 37.0 41.0 31.0 41.0 52-53 36.83048034105484 40.0 37.0 41.0 31.0 41.0 54-55 36.69275391912436 40.0 36.0 41.0 31.0 41.0 56-57 36.540669837786446 40.0 36.0 41.0 31.0 41.0 58-59 36.36601343311315 40.0 35.5 41.0 31.0 41.0 60-61 36.21577554877309 39.5 35.0 41.0 31.0 41.0 62-63 36.05935627208449 39.0 35.0 41.0 30.5 41.0 64-65 35.833256119957355 39.0 35.0 41.0 30.0 41.0 66-67 35.57858001076623 38.5 35.0 41.0 30.0 41.0 68-69 35.29371363942394 37.5 35.0 41.0 29.0 41.0 70-71 35.0070521829816 37.0 35.0 40.0 29.0 41.0 72-73 34.69612151337545 37.0 35.0 39.5 29.0 41.0 74-75 34.36967674620148 36.0 35.0 39.0 29.0 41.0 76-77 34.04118637009344 36.0 35.0 39.0 29.0 41.0 78-79 33.723649424751954 35.0 35.0 38.0 28.5 40.0 80-81 33.46036247147931 35.0 34.5 37.0 29.0 39.0 82-83 33.25105887234564 35.0 34.5 37.0 29.0 39.0 84-85 32.98478917475183 35.0 34.0 36.5 29.0 38.5 86-87 32.73834472482412 35.0 34.0 36.0 29.0 37.0 88-89 32.50654270059154 35.0 34.0 36.0 28.0 37.0 90-91 32.31904021423691 35.0 34.0 35.5 27.0 37.0 92-93 32.15877797746947 35.0 34.0 35.0 27.0 36.0 94-95 32.01823448426585 35.0 34.0 35.0 27.0 36.0 96-97 31.873595115213266 35.0 34.0 35.0 26.5 36.0 98-99 31.75987340867009 35.0 34.0 35.0 26.0 36.0 100 31.65780332645295 35.0 34.0 35.0 25.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02312297780243 1101 2 0.0629395549686862 1101 3 0.08079222937201536 1101 4 0.06007916402389668 1101 5 0.06067724664465857 1101 6 0.06820578892942564 1101 7 0.07383933936564802 1101 8 0.09780380787884724 1101 9 0.06604478511245304 1101 10-11 0.07193024927517655 1101 12-13 0.08631791946739042 1101 14-15 0.09697318600263571 1101 16-17 0.004836376738808212 1101 18-19 0.015597545457296746 1101 20-21 0.09074208812060647 1101 22-23 0.045223770042838396 1101 24-25 0.07198940340668969 1101 26-27 0.13237297321663988 1101 28-29 0.09806385466578149 1101 30-31 0.08190917754532023 1101 32-33 0.0149158751949372 1101 34-35 0.03998797401757059 1101 36-37 0.046249072752246434 1101 38-39 0.048347531039048874 1101 40-41 -0.033025366519716215 1101 42-43 -0.06128973882874078 1101 44-45 -0.0889725402136392 1101 46-47 -0.07344232802555695 1101 48-49 0.0034877881446035985 1101 50-51 -0.08256053289127863 1101 52-53 -0.09931063217990754 1101 54-55 -0.0947137129375335 1101 56-57 -0.138461136638945 1101 58-59 -0.0973982723965392 1101 60-61 -0.002634661695104512 1101 62-63 -0.0576569010587491 1101 64-65 -0.12794569043380477 1101 66-67 -0.12564485996507813 1101 68-69 -0.13629184292857133 1101 70-71 -0.11179754727650248 1101 72-73 -0.11618363872542403 1101 74-75 -0.051867793515391725 1101 76-77 -0.12492787512598369 1101 78-79 -0.09784767001547579 1101 80-81 -0.06197495372842354 1101 82-83 -0.10411078006297458 1101 84-85 -0.10253434090750346 1101 86-87 -0.08989755794092247 1101 88-89 -0.13638972489857082 1101 90-91 -0.14980745596882628 1101 92-93 -0.1837398721473278 1101 94-95 -0.19245006661309816 1101 96-97 -0.14770146478597468 1101 98-99 -0.0883995446549406 1101 100 -0.033043883327351864 1102 1 0.005844091478770963 1102 2 0.03096998917553151 1102 3 0.02393666223960622 1102 4 0.033377804285350976 1102 5 0.022857144651773353 1102 6 -0.032871326635856235 1102 7 -0.0012068847488464485 1102 8 0.021142238357782617 1102 9 0.03234593771698968 1102 10-11 0.03047805625327271 1102 12-13 -0.02009676955675843 1102 14-15 -0.0046752190155530116 1102 16-17 0.006603277899159821 1102 18-19 0.056837872749014196 1102 20-21 0.04093009221331556 1102 22-23 0.05684843210708124 1102 24-25 0.05651547505656396 1102 26-27 0.08261680542060645 1102 28-29 0.005458760393572959 1102 30-31 0.008459293855189287 1102 32-33 -0.010345868853399054 1102 34-35 -0.047571398983549784 1102 36-37 -0.05947281673963545 1102 38-39 -0.030853975492298957 1102 40-41 -0.03880069483586368 1102 42-43 -0.15113175542445134 1102 44-45 -0.1859318836063366 1102 46-47 -0.2662063801563619 1102 48-49 -0.1967739246712199 1102 50-51 -0.2168750199035543 1102 52-53 -0.13087639285392783 1102 54-55 -0.10490636442770551 1102 56-57 -0.03752008550258523 1102 58-59 -0.08796486760322608 1102 60-61 -0.15637851826043203 1102 62-63 -0.11926237074572299 1102 64-65 -0.08895326056952513 1102 66-67 -0.0423843452283279 1102 68-69 -0.10738565539358547 1102 70-71 -0.10665659808304184 1102 72-73 -0.07530631127879417 1102 74-75 -0.10404102962817063 1102 76-77 -0.10690098580900553 1102 78-79 -0.12259396833841407 1102 80-81 -0.13004069628758685 1102 82-83 -0.11561911664448843 1102 84-85 -0.1389347223189148 1102 86-87 -0.12827909552987649 1102 88-89 -0.09794672173035224 1102 90-91 -0.11596504834209753 1102 92-93 -0.13858703273739792 1102 94-95 -0.13886490243003635 1102 96-97 -0.05798584615740765 1102 98-99 -0.05087148224488658 1102 100 -0.020333095118751032 1103 1 -0.054768407802576746 1103 2 -0.06872141868474557 1103 3 -0.042245755439594745 1103 4 -0.0178220995682139 1103 5 -0.08244564934408771 1103 6 -0.03891628916198897 1103 7 -0.10378069793362954 1103 8 -0.026955169235826304 1103 9 -0.03859594261861332 1103 10-11 -0.06233703127546164 1103 12-13 -0.02510058911461499 1103 14-15 -0.09311669874100659 1103 16-17 -0.06699301165573956 1103 18-19 -0.10713039403410818 1103 20-21 -0.08079051615143129 1103 22-23 -0.09063596550369368 1103 24-25 -0.10050618113307763 1103 26-27 -0.14712343712284337 1103 28-29 -0.10432906301502243 1103 30-31 -0.12819005177412635 1103 32-33 -0.10838844484394627 1103 34-35 -0.07378050769629851 1103 36-37 -0.08179161862782536 1103 38-39 -0.14478551458145006 1103 40-41 -0.2603193314647214 1103 42-43 -0.270052970392463 1103 44-45 -0.270606374097504 1103 46-47 -0.29200662186801196 1103 48-49 -0.26620360872131243 1103 50-51 -0.3618377753919191 1103 52-53 -0.2549190569175437 1103 54-55 -0.21105528681697194 1103 56-57 -0.18459936227588258 1103 58-59 -0.2944438652338093 1103 60-61 -0.3000064009715615 1103 62-63 -0.3225007992285569 1103 64-65 -0.27126701048849355 1103 66-67 -0.2809728733438277 1103 68-69 -0.18488264928841147 1103 70-71 -0.210551859017869 1103 72-73 -0.3108656761985529 1103 74-75 -0.2818914167215496 1103 76-77 -0.30420778468312903 1103 78-79 -0.22084811847609132 1103 80-81 -0.19945937120376556 1103 82-83 -0.2498533182315157 1103 84-85 -0.24007580375837279 1103 86-87 -0.20121531105446167 1103 88-89 -0.2266263718356072 1103 90-91 -0.1997005514533967 1103 92-93 -0.19611205099420914 1103 94-95 -0.1960475060412783 1103 96-97 -0.21627976067514965 1103 98-99 -0.24922910295087064 1103 100 -0.1978781010697972 1104 1 -0.12677758048592125 1104 2 -0.09755415501224007 1104 3 -0.09998191340852713 1104 4 -0.043970368114045755 1104 5 -0.08463438149355085 1104 6 -0.11984988383623829 1104 7 -0.1014775978543625 1104 8 -0.13907893870592147 1104 9 -0.14512994167945692 1104 10-11 -0.15666891368803704 1104 12-13 -0.11426554157412028 1104 14-15 -0.16450247841788013 1104 16-17 -0.13307071779227897 1104 18-19 -0.10194969028324863 1104 20-21 -0.0707033888582842 1104 22-23 -0.07519331318002287 1104 24-25 -0.07018890185228344 1104 26-27 -0.1457092313545374 1104 28-29 -0.12161475543564393 1104 30-31 -0.09984103306185688 1104 32-33 -0.11782667490063403 1104 34-35 -0.11873857722861203 1104 36-37 -0.0447992996240103 1104 38-39 -0.1029882137856788 1104 40-41 -0.15493730625267688 1104 42-43 -0.1610517360180097 1104 44-45 -0.12709520721041656 1104 46-47 -0.14279949010429505 1104 48-49 -0.1905323313338556 1104 50-51 -0.21483852287018834 1104 52-53 -0.15277226976637337 1104 54-55 -0.14361794034184783 1104 56-57 -0.15148491224818628 1104 58-59 -0.16348756044285295 1104 60-61 -0.14917977330826204 1104 62-63 -0.11780463649358097 1104 64-65 -0.08513364367826881 1104 66-67 -0.1669147934463524 1104 68-69 -0.21214196382673833 1104 70-71 -0.21897275031518149 1104 72-73 -0.18707215493289908 1104 74-75 -0.17780827577739444 1104 76-77 -0.1903229973251399 1104 78-79 -0.19134387166609912 1104 80-81 -0.20490323017266832 1104 82-83 -0.19797291632965397 1104 84-85 -0.28427787342436517 1104 86-87 -0.2155422100767268 1104 88-89 -0.22549613334244611 1104 90-91 -0.2413142823374912 1104 92-93 -0.28646534921031375 1104 94-95 -0.2505778806861585 1104 96-97 -0.25540043415876923 1104 98-99 -0.25340428919077596 1104 100 -0.21788924967254886 1105 1 -0.1384031094807554 1105 2 -0.1057463440621973 1105 3 -0.10072856078418368 1105 4 -0.040890870068963636 1105 5 -0.1362464374040968 1105 6 -0.06061346646259835 1105 7 -0.061650752823631194 1105 8 -0.05930192877673335 1105 9 -0.05394584398571567 1105 10-11 -0.07264847727744694 1105 12-13 -0.09060182253337956 1105 14-15 -0.1179937987355899 1105 16-17 -0.15082946651306628 1105 18-19 -0.13186845376893075 1105 20-21 -0.08996975585528588 1105 22-23 -0.08309475560682245 1105 24-25 -0.15873240241231912 1105 26-27 -0.09299224557678087 1105 28-29 -0.10607896395219996 1105 30-31 -0.09548253993376221 1105 32-33 -0.15581595223215317 1105 34-35 -0.12304324500222208 1105 36-37 -0.08233026901356055 1105 38-39 -0.041335112753010606 1105 40-41 -0.056936900230084575 1105 42-43 -0.20319320585507228 1105 44-45 -0.12350978073613561 1105 46-47 -0.18099388689087448 1105 48-49 -0.2466869481333589 1105 50-51 -0.2347915070211073 1105 52-53 -0.171793880915331 1105 54-55 -0.12842854491749733 1105 56-57 -0.10200574581514132 1105 58-59 -0.07498513161858966 1105 60-61 -0.1295904242813677 1105 62-63 -0.1625884677398588 1105 64-65 -0.1382015558157974 1105 66-67 -0.16900356108466497 1105 68-69 -0.18874535310763463 1105 70-71 -0.15359107726826693 1105 72-73 -0.09279709140327697 1105 74-75 -0.13564593366398014 1105 76-77 -0.1574786015507783 1105 78-79 -0.09188529193599493 1105 80-81 -0.09412516344244892 1105 82-83 -0.15996817916020944 1105 84-85 -0.11667575199968638 1105 86-87 -0.1545452849697142 1105 88-89 -0.11983449548894498 1105 90-91 -0.10120944131201526 1105 92-93 -0.09734035241367422 1105 94-95 -0.07486285794898251 1105 96-97 -0.1384834362344236 1105 98-99 -0.14707777364596808 1105 100 -0.11221004382139554 1106 1 -0.11563381567582809 1106 2 -0.0875591480945559 1106 3 -0.0847293244817422 1106 4 -0.014726295462274663 1106 5 -0.01767162934348221 1106 6 -0.05763567599152708 1106 7 -0.053600136918937835 1106 8 -0.028539448050004523 1106 9 -0.04634868079664045 1106 10-11 -0.07545734953905026 1106 12-13 -0.08129571689624981 1106 14-15 -0.13580766256455234 1106 16-17 -0.12833509682838695 1106 18-19 -0.07533084510594534 1106 20-21 -0.07483736469559688 1106 22-23 -0.08430392882080184 1106 24-25 -0.02663182930081831 1106 26-27 -0.03596633014304018 1106 28-29 -0.08778023500813248 1106 30-31 -0.0896454481957889 1106 32-33 -0.07818179649478907 1106 34-35 -0.019123006713655855 1106 36-37 -0.03035511450071482 1106 38-39 -0.07020252454395859 1106 40-41 -0.2176226985355285 1106 42-43 -0.2181997870006782 1106 44-45 -0.14404217336581127 1106 46-47 -0.16022129294650256 1106 48-49 -0.06375606684551371 1106 50-51 -0.06925535132013749 1106 52-53 -0.1085204271403768 1106 54-55 -0.12636900567468956 1106 56-57 -0.0652035094156318 1106 58-59 -0.10835275609219508 1106 60-61 -0.1523111994915567 1106 62-63 -0.14956957398364779 1106 64-65 -0.13378576577964907 1106 66-67 -0.17640681031092242 1106 68-69 -0.17127053611038434 1106 70-71 -0.08705925058677622 1106 72-73 -0.06334118566218194 1106 74-75 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1107 30-31 -0.16431863340762476 1107 32-33 -0.12050290846068634 1107 34-35 -0.15046154711652093 1107 36-37 -0.2319024661816158 1107 38-39 -0.14115407450939443 1107 40-41 -0.21665178721449507 1107 42-43 -0.20166407832818578 1107 44-45 -0.15633322192132226 1107 46-47 -0.1885123586206987 1107 48-49 -0.17372194894758763 1107 50-51 -0.13942557373445652 1107 52-53 -0.1272769270732823 1107 54-55 -0.14210404209958938 1107 56-57 -0.2638011610987263 1107 58-59 -0.23625214839011477 1107 60-61 -0.2421915852158989 1107 62-63 -0.2496158203025587 1107 64-65 -0.18066987085579456 1107 66-67 -0.19427546479092683 1107 68-69 -0.1577373914503113 1107 70-71 -0.15342444157622737 1107 72-73 -0.1355990699113292 1107 74-75 -0.14066147195215706 1107 76-77 -0.15030947664824623 1107 78-79 -0.14338800607200852 1107 80-81 -0.08800969889220056 1107 82-83 -0.13080889347448732 1107 84-85 -0.10332908258320828 1107 86-87 -0.10032686648488465 1107 88-89 -0.12245936704876215 1107 90-91 -0.0935124406760579 1107 92-93 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0.4789848119012632 2201 42-43 0.501221428937491 2201 44-45 0.5005385383323571 2201 46-47 0.48069437103991675 2201 48-49 0.5231388973765121 2201 50-51 0.46529352826310344 2201 52-53 0.5076960700643482 2201 54-55 0.5229806939526043 2201 56-57 0.49997776931121507 2201 58-59 0.4899476764193196 2201 60-61 0.5128799861481994 2201 62-63 0.4631950096683468 2201 64-65 0.4206214318330268 2201 66-67 0.39199269949070725 2201 68-69 0.3841211882975415 2201 70-71 0.4520549408225989 2201 72-73 0.44849278065652953 2201 74-75 0.4017801717998708 2201 76-77 0.42472600944057604 2201 78-79 0.39318584778762045 2201 80-81 0.41378369563796724 2201 82-83 0.39197331050483797 2201 84-85 0.3916564857156217 2201 86-87 0.35664662631301525 2201 88-89 0.37295179922696775 2201 90-91 0.3648375992063322 2201 92-93 0.3809988002944422 2201 94-95 0.3944621066604199 2201 96-97 0.4158494479176227 2201 98-99 0.39824257283398623 2201 100 0.42714910908063786 2202 1 0.24395370066796573 2202 2 0.24890230449339867 2202 3 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70-71 0.2300629028570924 2214 72-73 0.19171024363043188 2214 74-75 0.16415105276215058 2214 76-77 0.13926262555847302 2214 78-79 0.14507660058421123 2214 80-81 0.16865102038590862 2214 82-83 0.1224877467050689 2214 84-85 0.14961342656676635 2214 86-87 0.13247176642574487 2214 88-89 0.12446680506910468 2214 90-91 0.1566121893917085 2214 92-93 0.1356659284200319 2214 94-95 0.12933739194838978 2214 96-97 0.19625034180955225 2214 98-99 0.21945452271047827 2214 100 0.2697003874372612 2215 1 0.10216473392659964 2215 2 0.09054200347795671 2215 3 0.07150740513801424 2215 4 0.003874569374389125 2215 5 0.05655539372239815 2215 6 0.03485547900115904 2215 7 0.006293656043624196 2215 8 0.02243112604146802 2215 9 -0.006680692798610721 2215 10-11 0.0278681862304353 2215 12-13 0.03743025056468241 2215 14-15 0.06623674591410378 2215 16-17 0.005046092763890897 2215 18-19 0.03282599376419171 2215 20-21 0.0653381519836671 2215 22-23 3.139813051973306E-4 2215 24-25 0.04511313053702537 2215 26-27 0.03315985763967433 2215 28-29 0.039923120776933274 2215 30-31 0.023198751992900668 2215 32-33 0.06926377247025073 2215 34-35 0.055888570796462034 2215 36-37 -0.002454964954289096 2215 38-39 -0.031173188539270313 2215 40-41 0.028598973147900608 2215 42-43 0.009069868847433327 2215 44-45 0.046271673278901915 2215 46-47 0.10717917633239438 2215 48-49 0.1743414302316495 2215 50-51 0.19595046008303996 2215 52-53 0.1461903785166001 2215 54-55 0.10713715503358401 2215 56-57 0.0909467594924891 2215 58-59 0.04923156270834994 2215 60-61 0.15071734755914434 2215 62-63 0.14297302943636936 2215 64-65 0.08778685605343384 2215 66-67 0.12024989791171237 2215 68-69 0.0873165595710148 2215 70-71 0.09571079136962624 2215 72-73 0.08638734300604511 2215 74-75 0.0648627657753309 2215 76-77 0.056445914697938804 2215 78-79 0.0979007103503804 2215 80-81 0.07934731237153159 2215 82-83 0.08924754504453603 2215 84-85 0.09001490454975425 2215 86-87 0.0637266359416202 2215 88-89 0.06220641385681347 2215 90-91 0.10334548367015373 2215 92-93 0.08734466879567293 2215 94-95 0.11697186108150959 2215 96-97 0.09441380511038489 2215 98-99 0.0729181239157981 2215 100 0.13284850429215567 2216 1 0.09932318198434587 2216 2 0.10167166016820062 2216 3 0.06417325296961707 2216 4 0.03706994733997249 2216 5 0.03722162270754126 2216 6 0.05178085659212428 2216 7 0.047569691381127654 2216 8 0.03183209912806717 2216 9 -0.022280525550606 2216 10-11 -8.245054061433166E-4 2216 12-13 0.03507553200235236 2216 14-15 0.04113488820281219 2216 16-17 0.016636780731715817 2216 18-19 -0.021846857620602123 2216 20-21 0.00875424651447787 2216 22-23 -0.03969870523737029 2216 24-25 -0.04200180910732598 2216 26-27 -0.01377953877548066 2216 28-29 0.017702834984675064 2216 30-31 -0.041587149433034654 2216 32-33 -0.02716599330066316 2216 34-35 0.03154782739323991 2216 36-37 0.040055668536744804 2216 38-39 0.021111718351114916 2216 40-41 -0.011905756814492463 2216 42-43 0.05008778773954958 2216 44-45 0.04383903313087245 2216 46-47 0.03680204330055403 2216 48-49 0.06748763419945192 2216 50-51 0.05321879303497212 2216 52-53 -0.016349068212662132 2216 54-55 -0.0027141410764386364 2216 56-57 -0.032707831198671045 2216 58-59 -0.07763403473217778 2216 60-61 -0.09247924207469538 2216 62-63 -0.09351358225922723 2216 64-65 -0.06505369109541448 2216 66-67 -0.012070630384044989 2216 68-69 -0.05210290572200904 2216 70-71 -0.06929577146762966 2216 72-73 -0.047646105182899134 2216 74-75 -0.042745760834733915 2216 76-77 -0.011391409764513583 2216 78-79 -0.022283440322759418 2216 80-81 -0.00991177816047184 2216 82-83 -0.01870493962062625 2216 84-85 0.034934963930474794 2216 86-87 0.004378565752809038 2216 88-89 0.03204834601748274 2216 90-91 -0.011247826627162283 2216 92-93 0.062331782534911895 2216 94-95 0.0014116399002332969 2216 96-97 0.024450364513782574 2216 98-99 0.028938831706017965 2216 100 0.05385842147904896 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50.0 3 341.0 4 94.0 5 154.0 6 389.0 7 886.0 8 1519.0 9 2523.0 10 3672.0 11 5311.0 12 8226.0 13 12354.0 14 13004.0 15 17212.0 16 22578.0 17 34373.0 18 40280.0 19 20509.0 20 12498.0 21 10994.0 22 10702.0 23 10902.0 24 11551.0 25 13416.0 26 16636.0 27 20780.0 28 26000.0 29 33265.0 30 41595.0 31 53088.0 32 66839.0 33 87682.0 34 122961.0 35 209300.0 36 330224.0 37 675008.0 38 1208374.0 39 817909.0 40 8479.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98536500982893 21.090052703791898 14.547994040450304 22.376588245928872 2 14.236956022528583 22.190725491302 40.920355292525116 22.6519631936443 3 16.44436555176199 28.981151363164837 32.137857409030026 22.436625676043146 4 10.835193588201259 18.620215435390282 40.0080520122729 30.536538964135563 5 11.046510309775677 39.46926187230756 35.551262457241506 13.93296536067526 6 26.72182890959438 40.325071669959144 18.789337907050875 14.163761513395597 7 23.60536277110078 35.655332582349324 23.842315515003985 16.89698913154591 8 24.764998572391818 35.15672720698909 21.487592901539347 18.590681319079742 9 25.952947823538473 15.767751448003311 22.57502877878256 35.70427194967565 10-11 22.623901031251776 27.69097847307863 29.774417764984978 19.910702730684612 12-13 24.36915832552387 26.118041291363497 29.9636828564652 19.54911752664743 14-15 21.569840451547236 26.9469386676033 29.024398380784945 22.458822500064517 16-17 19.028722750021874 30.333819903554605 29.563012102568752 21.07444524385477 18-19 19.012308284578708 27.722400211799837 33.85323998731518 19.412051516306285 20-21 20.698153274258384 26.736495254650556 33.63466272945591 18.930688741635148 22-23 20.109321620056825 26.855278081001334 33.790638233158774 19.244762065783068 24-25 19.79461318161235 26.908473033994586 33.78126340350228 19.51565038089079 26-27 19.513008504480858 27.03015934602841 33.80339156994625 19.65344057954448 28-29 19.433780386585052 26.97140714829476 33.972245207909154 19.62256725721103 30-31 19.698952681845512 27.264552324111683 33.609342472734376 19.42715252130843 32-33 19.469567109071757 27.150719705591463 33.82924717949131 19.55046600584547 34-35 19.724776128346996 27.030990602243733 33.57531568512198 19.668917584287296 36-37 19.933760177498954 27.167618123445063 33.103041598296315 19.795580100759665 38-39 19.805500953132952 27.23420985643459 33.223714441317554 19.73657474911491 40-41 19.769618321763893 27.116124954993744 33.15324536026407 19.961011362978294 42-43 19.804406549257216 27.233674412678944 32.95043264693208 20.01148639113176 44-45 20.044336479866292 27.339033198352432 32.53934802789656 20.077282293884718 46-47 20.199636274184705 27.64126100008737 31.90538344924167 20.253719276486258 48-49 20.408847961261657 27.719925008913123 31.326025915533812 20.545201114291412 50-51 20.396161407410094 27.903291919961003 31.066852583702953 20.633694088925953 52-53 20.39301302896945 28.031438826613474 30.992601436758203 20.58294670765887 54-55 20.455660748622922 28.236012890396132 30.85602694504712 20.452299415933826 56-57 20.418005135490226 27.947568951182628 30.812185987534424 20.822239925792722 58-59 20.337969939483667 27.678385962526956 31.20235770730338 20.781286390686 60-61 20.479297154718417 27.908832347843255 30.982911793350233 20.628958704088095 62-63 20.29749494571562 27.786321008462394 31.20782509853321 20.708358947288776 64-65 20.352872097933105 27.634870401391474 31.284600271577588 20.72765722909783 66-67 20.317710742326327 27.750831326827075 31.079160039509894 20.8522978913367 68-69 20.628736152536494 27.907935863690454 30.802275417922857 20.6610525658502 70-71 20.536092410590417 27.72607263551366 30.858133589279664 20.87970136461626 72-73 20.45099931540116 27.888703716246244 30.90470278364083 20.755594184711768 74-75 20.50364231674106 28.00745433633595 30.653282969689155 20.83562037723383 76-77 20.538223030339957 27.913331093286697 30.55799192450178 20.99045395187156 78-79 20.570341865460716 27.909799376100853 30.758361385378898 20.761497373059537 80-81 20.44229582209165 27.92814455386886 30.73485628923363 20.894703334805858 82-83 20.34824858489628 27.899908274136205 30.86990764718753 20.88193549377999 84-85 20.3134918027132 27.919886071649874 30.57649789670157 21.190124228935353 86-87 20.406075994880144 27.951127462218277 30.465758543195413 21.177037999706165 88-89 20.290579049388267 27.763974991071027 30.63938746428883 21.306058495251875 90-91 20.178088426077757 27.840070399440027 30.556441353628145 21.42539982085407 92-93 20.198918779007364 27.90076142713517 30.577376340793847 21.322943453063623 94-95 20.094817111091974 27.760215789624752 30.730029816353223 21.41493728293005 96-97 20.143428240873334 27.867365700470014 30.51465094365157 21.474555115005074 98-99 20.010058753696498 27.806965088948843 30.554163052523315 21.628813104831348 100 20.08481071428931 27.899864741967356 30.446804717816295 21.56851982592704 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4284.0 1 2509.5 2 3871.0 3 6390.5 4 3548.0 5 1853.5 6 4415.5 7 11560.5 8 20044.5 9 21339.5 10 13291.5 11 6071.0 12 4758.5 13 4977.0 14 5558.5 15 6051.5 16 6582.0 17 7212.5 18 7997.0 19 8934.0 20 10222.5 21 12746.0 22 16859.0 23 22994.0 24 29772.5 25 36281.0 26 45539.0 27 57368.5 28 72312.0 29 86827.0 30 94662.0 31 101927.5 32 110685.0 33 119073.5 34 127094.5 35 131206.0 36 131952.0 37 133063.5 38 133309.5 39 131426.5 40 130304.5 41 127663.5 42 122385.0 43 118507.5 44 116192.5 45 113726.5 46 111568.5 47 108603.5 48 105368.5 49 104198.5 50 103345.0 51 98516.5 52 91025.0 53 85378.0 54 81642.0 55 79057.5 56 73195.0 57 65432.0 58 60621.5 59 55796.0 60 49897.5 61 42534.0 62 36015.5 63 29753.0 64 22637.0 65 18077.0 66 14727.5 67 11356.0 68 8686.5 69 6512.0 70 4876.5 71 3939.5 72 3242.5 73 2765.0 74 2619.5 75 2134.5 76 1350.0 77 1157.5 78 1046.0 79 685.5 80 338.5 81 136.5 82 83.0 83 65.5 84 37.0 85 15.5 86 5.5 87 5.5 88 7.0 89 6.5 90 3.0 91 1.5 92 2.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00596725112156625 2 0.004783872207162816 3 9.567744414325633E-4 4 0.0 5 0.050331371274307736 6 0.0 7 0.0 8 0.0 9 5.0356549549082276E-5 10-11 0.0 12-13 0.0 14-15 1.2589137387270569E-5 16-17 1.2589137387270569E-5 18-19 8.812396171089398E-5 20-21 0.0 22-23 6.294568693635284E-5 24-25 3.776741216181171E-5 26-27 1.7624792342178796E-4 28-29 8.812396171089398E-5 30-31 2.5178274774541136E-4 32-33 0.0013596268378252216 34-35 9.69363578819834E-4 36-37 0.0012337354639525159 38-39 9.819527162071044E-4 40-41 2.014261981963291E-4 42-43 5.413329076526345E-4 44-45 1.2589137387270568E-4 46-47 1.2589137387270568E-4 48-49 1.5106964864724685E-4 50-51 3.5249584684357593E-4 52-53 7.175808310744224E-4 54-55 9.819527162071044E-4 56-57 0.002517827477454114 58-59 5.791003198144461E-4 60-61 0.001258913738727057 62-63 0.001195968051790704 64-65 0.0017373009594433386 66-67 3.776741216181171E-4 68-69 3.021392972944937E-4 70-71 3.021392972944937E-4 72-73 2.2660447297087027E-4 74-75 7.553482432362342E-4 76-77 8.686504797216693E-4 78-79 0.0010700766779179983 80-81 9.441853040452927E-4 82-83 0.0015358747612470094 84-85 0.0014855182116979273 86-87 0.0020520293941251027 88-89 0.0014225725247615745 90-91 0.0013973942499870332 92-93 0.0014351616621488448 94-95 8.057047927853164E-4 96-97 1.3848051125997627E-4 98-99 3.2731757206903476E-4 100 2.5178274774541136E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3971678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.81376474835036 #Duplication Level Percentage of deduplicated Percentage of total 1 72.58134073095025 40.51037876677117 2 16.301092700752132 18.196507062816615 3 5.380604878296565 9.009354446432125 4 2.2537049531272646 5.031510322641484 5 1.0888380228407333 3.038607462794581 6 0.6073081156448085 2.0337691377817975 7 0.38808189836306234 1.5162218244830423 8 0.2451310606880493 1.0945349883005102 9 0.19008953769273904 0.9548651464114695 >10 0.8680305715803036 8.458969394231271 >50 0.05537880020936311 2.1157817633821168 >100 0.03705027535341771 3.874937390830274 >500 0.002307718723092194 0.8568394691995617 >1k 6.787407250518922E-4 0.5337725289081796 >5k 2.2624690835062954E-4 0.7603133183241393 >10k+ 1.3574814501037772E-4 2.013636976691644 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34091 0.858352565338882 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26922 0.6778495134801965 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18718 0.47128694722986103 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8184 0.20605900075484468 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5891 0.14832521669682186 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5404 0.1360633968816203 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5355 0.1348296614176678 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5271 0.13271468633660635 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.5178274774541138E-5 0.0 5 0.0 0.0 0.0 2.5178274774541138E-5 0.0 6 0.0 0.0 0.0 2.5178274774541138E-5 0.0 7 0.0 0.0 0.0 5.0356549549082276E-5 0.0 8 0.0 0.0 0.0 5.0356549549082276E-5 0.0 9 0.0 0.0 0.0 5.0356549549082276E-5 0.0 10-11 0.0 0.0 0.0 5.0356549549082276E-5 0.0 12-13 0.0 0.0 0.0 5.0356549549082276E-5 0.0 14-15 0.0 0.0 0.0 5.0356549549082276E-5 0.0 16-17 0.0 0.0 0.0 6.294568693635284E-5 0.0 18-19 0.0 0.0 0.0 7.553482432362341E-5 0.0 20-21 0.0 0.0 0.0 1.6365878603451738E-4 0.0 22-23 0.0 0.0 0.0 3.399067094563054E-4 0.0 24-25 0.0 0.0 0.0 7.679373806235047E-4 0.0 26-27 0.0 0.0 0.0 0.0014351616621488448 0.0 28-29 0.0 0.0 0.0 0.002807377637361337 0.0 30-31 0.0 0.0 0.0 0.00662188626570432 0.0 32-33 0.0 0.0 0.0 0.014439740583199343 0.0 34-35 0.0 0.0 0.0 0.026437188513268196 0.0 36-37 0.0 0.0 0.0 0.04578669267750306 0.0 38-39 0.0 0.0 0.0 0.0714811220849223 0.0 40-41 0.0 0.0 0.0 0.10046131635041913 0.0 42-43 0.0 0.0 0.0 0.12793081412944352 0.0 44-45 0.0 2.5178274774541138E-5 0.0 0.16184595025075044 0.0 46-47 0.0 2.5178274774541138E-5 0.0 0.20541695474809388 0.0 48-49 0.0 2.5178274774541138E-5 0.0 0.25431316436025275 0.0 50-51 0.0 2.5178274774541138E-5 0.0 0.30628112349490566 0.0 52-53 0.0 2.5178274774541138E-5 0.0 0.355894913938139 0.0 54-55 0.0 2.5178274774541138E-5 0.0 0.4105695376110551 0.0 56-57 0.0 2.5178274774541138E-5 0.0 0.4780599031442126 0.0 58-59 0.0 2.5178274774541138E-5 0.0 0.5576484297065372 0.0 60-61 0.0 2.5178274774541138E-5 0.0 0.6428894789557462 0.0 62-63 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.7290495352342259 0.0 64-65 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.820106765956354 0.0 66-67 2.5178274774541138E-5 2.5178274774541138E-5 0.0 0.9271647902977029 0.0 68-69 2.5178274774541138E-5 2.5178274774541138E-5 0.0 1.052288226789785 0.0 70-71 2.5178274774541138E-5 2.5178274774541138E-5 0.0 1.191863993002454 0.0 72-73 2.5178274774541138E-5 2.5178274774541138E-5 0.0 1.3380490563434397 0.0 74-75 2.5178274774541138E-5 2.5178274774541138E-5 0.0 1.4905286883780609 0.0 76-77 2.5178274774541138E-5 2.5178274774541138E-5 0.0 1.6583293006129902 0.0 78-79 2.5178274774541138E-5 2.5178274774541138E-5 0.0 1.8536119997643312 0.0 80-81 2.5178274774541138E-5 2.5178274774541138E-5 0.0 2.0745010043613807 0.0 82-83 2.5178274774541138E-5 2.5178274774541138E-5 0.0 2.3032582198254743 0.0 84-85 2.5178274774541138E-5 2.5178274774541138E-5 0.0 2.5409285445597556 0.0 86-87 2.5178274774541138E-5 2.5178274774541138E-5 0.0 2.793831725532634 0.0 88 2.5178274774541138E-5 2.5178274774541138E-5 0.0 3.000192865584773 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11710 0.0 62.933647 1 GTATCAA 23645 0.0 45.746655 1 ATCAACG 30665 0.0 35.024284 3 TATCAAC 30905 0.0 34.861435 2 TCAACGC 30955 0.0 34.72654 4 CAACGCA 31935 0.0 33.64615 5 AACGCAG 32900 0.0 32.783615 6 GTGGTAT 3935 0.0 32.149807 1 TGGTATC 4040 0.0 31.079845 2 CGCAGAG 37240 0.0 28.773663 8 ACGCAGA 37330 0.0 28.754652 7 GCAGAGT 39850 0.0 26.759388 9 CATGGGT 10725 0.0 26.350767 4 GTACATG 22100 0.0 26.110975 1 TACATGG 21995 0.0 25.721163 2 ACATGGG 22110 0.0 24.649652 3 ATGGGTG 5490 0.0 23.297678 5 GAGTACT 27080 0.0 23.13404 12-13 CAGAGTA 39505 0.0 22.41294 10-11 AGAGTAC 37980 0.0 22.217768 10-11 >>END_MODULE