FastQCFastQC Report
Wed 25 May 2016
SRR2431381_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431381_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325870
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA222580.6692384248332015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44050.043.795611
GTATCAA71100.035.4721761
ATCAACG81250.029.8799383
TCAACGC81350.029.7840274
GTACATG159700.028.285181
CAACGCA87300.027.8618555
TATCAAC89850.027.5959852
TACATGG161950.026.9600322
AACGCAG92200.026.2494146
CATGGGT77350.025.8501054
ACATGGG164150.025.5668563
TACCTGG23000.023.524662
CGCAGAG102950.023.326228
ACGCAGA104450.022.9912347
ATGGGTA34600.021.3480035
ATGGGTG43650.020.8020825
CTGTCGC7150.020.375989
GTACCTG27400.020.0922181
GCAGAGT117650.019.8131089
CATGGGG70200.019.1003574
Produced by FastQC (version 0.11.5)