##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431381_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3325870
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.566922339117284	31.0	31.0	34.0	26.0	34.0
2	30.745088653495177	33.0	31.0	34.0	27.0	34.0
3	30.832591171633286	34.0	31.0	34.0	27.0	34.0
4	34.09737662626621	37.0	35.0	37.0	30.0	37.0
5	34.131329246182204	37.0	35.0	37.0	30.0	37.0
6	34.226177511448135	37.0	35.0	37.0	30.0	37.0
7	34.138933872941514	37.0	35.0	37.0	30.0	37.0
8	34.126672720220576	37.0	35.0	37.0	30.0	37.0
9	35.57229476798552	39.0	37.0	39.0	30.0	39.0
10-11	35.58481645404059	39.0	37.0	39.0	30.0	39.0
12-13	35.50302402078253	39.0	37.0	39.0	29.5	39.0
14-15	36.833492138899	40.0	38.0	41.0	29.5	41.0
16-17	36.849799150297514	40.0	38.0	41.0	30.0	41.0
18-19	36.86330839750201	40.0	38.0	41.0	30.0	41.0
20-21	36.93927633972464	40.0	38.0	41.0	30.0	41.0
22-23	36.90675402225583	40.0	38.0	41.0	29.5	41.0
24-25	36.948969141908734	40.0	38.0	41.0	30.0	41.0
26-27	36.98543208243257	40.0	38.0	41.0	30.0	41.0
28-29	36.953830426324544	40.0	38.0	41.0	30.0	41.0
30-31	36.882976484348454	40.0	38.0	41.0	30.0	41.0
32-33	36.83080066268375	40.0	38.0	41.0	30.0	41.0
34-35	36.75643861606136	40.0	37.5	41.0	30.0	41.0
36-37	36.62234287569869	40.0	37.0	41.0	29.5	41.0
38-39	36.49431441998635	40.0	37.0	41.0	29.0	41.0
40-41	36.434169104625255	40.0	37.0	41.0	29.0	41.0
42-43	36.25823122972335	40.0	36.5	41.0	28.0	41.0
44-45	36.094420707965135	40.0	36.0	41.0	28.0	41.0
46-47	35.959195488699194	40.0	36.0	41.0	27.5	41.0
48-49	35.817974093996455	40.0	35.0	41.0	27.0	41.0
50-51	35.67843872430372	40.0	35.0	41.0	27.0	41.0
52-53	35.516466518535	40.0	35.0	41.0	27.0	41.0
54-55	35.347110680814346	39.0	35.0	41.0	27.0	41.0
56-57	35.15329928710382	39.0	35.0	41.0	26.0	41.0
58-59	34.93710953825615	39.0	34.0	41.0	26.0	41.0
60-61	34.69614837621434	38.5	34.0	40.5	26.0	41.0
62-63	34.60045491856266	38.0	34.0	40.0	26.0	41.0
64-65	34.35230811787592	38.0	34.0	40.0	25.0	41.0
66-67	34.09176350849552	37.5	34.0	40.0	25.0	41.0
68-69	33.80015965747308	37.0	33.5	40.0	24.0	41.0
70-71	33.4717675375165	36.5	33.0	39.5	23.5	41.0
72-73	33.13422969027653	36.0	33.0	39.0	22.5	41.0
74-75	32.84976412186886	36.0	33.0	39.0	22.0	41.0
76-77	32.583374876348145	35.0	33.0	38.5	22.0	40.0
78-79	32.2336898315328	35.0	32.5	37.0	20.5	39.5
80-81	31.899527642391313	35.0	32.0	37.0	20.0	39.0
82-83	31.56815810599933	35.0	32.0	36.5	18.5	39.0
84-85	31.256657506156284	35.0	32.0	36.0	18.0	37.5
86-87	30.969589310466134	35.0	32.0	36.0	15.0	37.0
88-89	30.698827675164694	35.0	31.5	35.5	9.5	37.0
90-91	30.46234819761446	35.0	31.0	35.0	4.5	36.0
92-93	30.226381668555895	35.0	31.0	35.0	2.0	36.0
94-95	30.006125314579343	35.0	31.0	35.0	2.0	36.0
96-97	29.79419595474267	35.0	31.0	35.0	2.0	36.0
98-99	29.57525790244357	34.5	31.0	35.0	2.0	35.0
100	29.399243205537196	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.04352659851735652
1101	2	0.042978599137132534
1101	3	0.07063264866721752
1101	4	0.07727762464866572
1101	5	0.08340116344118798
1101	6	0.11019343810418292
1101	7	0.08268230527903597
1101	8	0.061066721423259196
1101	9	0.07476495064249633
1101	10-11	0.06319231726951813
1101	12-13	0.006571936551516444
1101	14-15	0.09605448666790295
1101	16-17	0.021009618757076964
1101	18-19	-0.14570410637735876
1101	20-21	-0.06510066552031901
1101	22-23	-0.015650354499022967
1101	24-25	-0.019053398303292113
1101	26-27	-0.026129349752615383
1101	28-29	-7.195377545627935E-4
1101	30-31	0.026568662013367828
1101	32-33	0.02148694589269695
1101	34-35	0.024464560539726676
1101	36-37	0.013122078090511025
1101	38-39	0.04671144121966364
1101	40-41	-0.03442428976941159
1101	42-43	-0.03965037891410361
1101	44-45	-0.09893437902476165
1101	46-47	-0.09749407792238429
1101	48-49	-0.025218582770342834
1101	50-51	-0.07245387546738868
1101	52-53	-0.13391214645639593
1101	54-55	-0.1149726869068175
1101	56-57	-0.14132895506731558
1101	58-59	-0.21324263478671668
1101	60-61	-0.1777154605778506
1101	62-63	-0.15716058147986445
1101	64-65	-0.12981581677762932
1101	66-67	-0.07154404233928346
1101	68-69	-0.08776119836718976
1101	70-71	-0.028634316813906935
1101	72-73	-0.07756705637255124
1101	74-75	-0.13272942373902907
1101	76-77	-0.10436001881328139
1101	78-79	-0.16535649652632145
1101	80-81	-0.1658264869143693
1101	82-83	-0.14421235991305892
1101	84-85	-0.12000404875560733
1101	86-87	-0.220149710875841
1101	88-89	-0.16200628021936936
1101	90-91	-0.20232875451365473
1101	92-93	-0.2581614113239574
1101	94-95	-0.27849963600629124
1101	96-97	-0.23634555999937845
1101	98-99	-0.2728299385485933
1101	100	-0.19625384394124623
1102	1	0.03867340385462725
1102	2	0.05773636791164094
1102	3	0.054662833920748
1102	4	0.1411695833571187
1102	5	0.10885412885232881
1102	6	0.12141666674342133
1102	7	0.027716826062352595
1102	8	0.06420791572936224
1102	9	0.18982063590224385
1102	10-11	0.07591739609419079
1102	12-13	0.033235176884879536
1102	14-15	0.059356284084692845
1102	16-17	0.046979169141977195
1102	18-19	0.03340118261649394
1102	20-21	-0.008015585327193264
1102	22-23	-0.02079127995113339
1102	24-25	-0.016581703997886166
1102	26-27	-0.0010556913616781571
1102	28-29	-0.04000049242122117
1102	30-31	-0.021038623860668793
1102	32-33	-0.02595612911272127
1102	34-35	0.006815617847777844
1102	36-37	-0.06329748739582186
1102	38-39	-0.005536016589623216
1102	40-41	-0.03949273177089907
1102	42-43	-0.06531155066134886
1102	44-45	-0.11903279150760682
1102	46-47	-0.23949460042525317
1102	48-49	-0.16838313851850017
1102	50-51	-0.13109363496401727
1102	52-53	-0.13555244089643992
1102	54-55	-0.16550283121022602
1102	56-57	-0.10688104397298304
1102	58-59	-0.28839659084533054
1102	60-61	-0.22428990047689012
1102	62-63	-0.09549571782822852
1102	64-65	-0.09080650226545828
1102	66-67	-0.03786031761259778
1102	68-69	-0.1299536076306964
1102	70-71	-0.13083777483605274
1102	72-73	-0.09061982259674295
1102	74-75	-0.08664737886598317
1102	76-77	-0.06789341338173926
1102	78-79	-0.12378602809835115
1102	80-81	-0.11863909101753478
1102	82-83	-0.12172966090927062
1102	84-85	-0.10088882806034505
1102	86-87	-0.08324715506963187
1102	88-89	-0.17012554979352856
1102	90-91	-0.15333216650578407
1102	92-93	-0.15292332435487666
1102	94-95	-0.22884903388393596
1102	96-97	-0.20810753246845337
1102	98-99	-0.2836539682552299
1102	100	-0.33949263259160034
1103	1	-0.31653155327358107
1103	2	-0.2643123649032155
1103	3	-0.2003252729027274
1103	4	-0.18046214946598838
1103	5	-0.2605038286140129
1103	6	-0.18890735616734844
1103	7	-0.2316218604031377
1103	8	-0.30509162393838807
1103	9	-0.3409291187429986
1103	10-11	-0.31959902068175694
1103	12-13	-0.3438715579313083
1103	14-15	-0.4031648751855883
1103	16-17	-0.39424926094497437
1103	18-19	-0.3908883624531825
1103	20-21	-0.42869881928154285
1103	22-23	-0.44160741439728923
1103	24-25	-0.3830822579360742
1103	26-27	-0.35799101804774836
1103	28-29	-0.40075776062627
1103	30-31	-0.415776795287357
1103	32-33	-0.387128679138975
1103	34-35	-0.39526506950291207
1103	36-37	-0.4292383902325909
1103	38-39	-0.3409578291770714
1103	40-41	-0.34302946556992
1103	42-43	-0.48054964442672343
1103	44-45	-0.5530805143818114
1103	46-47	-0.7129248110867081
1103	48-49	-0.6690026678634737
1103	50-51	-0.8004245827893612
1103	52-53	-0.7844907661337501
1103	54-55	-0.7179952179965312
1103	56-57	-0.7897018642138605
1103	58-59	-0.7444841464109686
1103	60-61	-0.8179937246434434
1103	62-63	-0.7030046987056835
1103	64-65	-0.6808601160592076
1103	66-67	-0.7142355069549922
1103	68-69	-0.755883739256312
1103	70-71	-0.7328171674421426
1103	72-73	-0.6349277469367607
1103	74-75	-0.6669377892063508
1103	76-77	-0.6575367280488678
1103	78-79	-0.6646784012080147
1103	80-81	-0.6228106802428215
1103	82-83	-0.6796906838667773
1103	84-85	-0.6650872826003003
1103	86-87	-0.7414400193698967
1103	88-89	-0.75837645904938
1103	90-91	-0.7865085535505045
1103	92-93	-0.7981168539307752
1103	94-95	-0.8492727725405729
1103	96-97	-0.7874772611212144
1103	98-99	-0.84155163367382
1103	100	-0.8372571208929216
1104	1	-0.2125512143928674
1104	2	-0.23864724546474747
1104	3	-0.1445363752084745
1104	4	-0.060468942175148754
1104	5	-0.06345205307531643
1104	6	-0.11738012872480397
1104	7	-0.11100881840155097
1104	8	-0.08714566371347132
1104	9	-0.058154297051615345
1104	10-11	-0.12973808253314445
1104	12-13	-0.18760009485723117
1104	14-15	-0.16929755943782965
1104	16-17	-0.1521396530352419
1104	18-19	-0.1776109746272212
1104	20-21	-0.17527038801998174
1104	22-23	-0.07123494751174775
1104	24-25	-0.1073963829973934
1104	26-27	-0.18171219620541734
1104	28-29	-0.06683006524153257
1104	30-31	-0.10770054712704535
1104	32-33	-0.1606663598061786
1104	34-35	-0.14073754016217777
1104	36-37	-0.2148496397137052
1104	38-39	-0.25366915790646516
1104	40-41	-0.1927318128283062
1104	42-43	-0.238482490949238
1104	44-45	-0.2712769557569672
1104	46-47	-0.4042098595790975
1104	48-49	-0.4124227025817717
1104	50-51	-0.49387858105345117
1104	52-53	-0.5057165671073776
1104	54-55	-0.4582674924219816
1104	56-57	-0.47844766594221966
1104	58-59	-0.48409554684117495
1104	60-61	-0.5710325185601874
1104	62-63	-0.48971315940231364
1104	64-65	-0.4439200428488945
1104	66-67	-0.3382557530020165
1104	68-69	-0.4373495492868784
1104	70-71	-0.5261197448978976
1104	72-73	-0.5181610764963693
1104	74-75	-0.5183812399026024
1104	76-77	-0.42802901077651256
1104	78-79	-0.44162611621613124
1104	80-81	-0.4724054762728578
1104	82-83	-0.51647509082553
1104	84-85	-0.4873046304247488
1104	86-87	-0.42685126130355755
1104	88-89	-0.5094180077870654
1104	90-91	-0.5275034967559122
1104	92-93	-0.5350611725843102
1104	94-95	-0.5520247631817305
1104	96-97	-0.5850965109042257
1104	98-99	-0.6109915181977605
1104	100	-0.685658003945111
1105	1	-0.3165561567992583
1105	2	-0.2994686747625721
1105	3	-0.27556336522196645
1105	4	-0.15202515611558454
1105	5	-0.2561562409003173
1105	6	-0.17580866044366417
1105	7	-0.27393958403386165
1105	8	-0.24147959140111652
1105	9	-0.40105153703290597
1105	10-11	-0.32513855982451645
1105	12-13	-0.30533843545615724
1105	14-15	-0.34887081078190363
1105	16-17	-0.3955542694055225
1105	18-19	-0.3210171163637767
1105	20-21	-0.3333484088879217
1105	22-23	-0.39523968652692787
1105	24-25	-0.3729161492330988
1105	26-27	-0.3673038781829163
1105	28-29	-0.36956432106699566
1105	30-31	-0.379568237755457
1105	32-33	-0.4153606289198635
1105	34-35	-0.4934643383099626
1105	36-37	-0.5239862270804068
1105	38-39	-0.5894786379707284
1105	40-41	-0.5978344745412656
1105	42-43	-0.6505372336932567
1105	44-45	-0.6369244347229213
1105	46-47	-0.6727329512610822
1105	48-49	-0.7810437610296219
1105	50-51	-0.6941751084875349
1105	52-53	-0.7610690610225888
1105	54-55	-0.7352619127231392
1105	56-57	-0.7474888119838923
1105	58-59	-0.7187711209128622
1105	60-61	-0.8424274093730091
1105	62-63	-0.7068714960689988
1105	64-65	-0.7479809178378787
1105	66-67	-0.7511040604481707
1105	68-69	-0.6712025754090831
1105	70-71	-0.6702463152475318
1105	72-73	-0.646946356396576
1105	74-75	-0.6410988515474259
1105	76-77	-0.6768576469028602
1105	78-79	-0.6571439332860791
1105	80-81	-0.6278189875509028
1105	82-83	-0.592530182724456
1105	84-85	-0.616965255242544
1105	86-87	-0.6053434036231629
1105	88-89	-0.6128993873218427
1105	90-91	-0.5492900851395603
1105	92-93	-0.6469777787985898
1105	94-95	-0.6921258825113519
1105	96-97	-0.6270875583011559
1105	98-99	-0.631423775365608
1105	100	-0.5024176090949766
1106	1	-0.29391708217787027
1106	2	-0.25643429895486847
1106	3	-0.229545407542755
1106	4	-0.1535431681098416
1106	5	-0.21043795246118435
1106	6	-0.16330589786785765
1106	7	-0.1784968040023216
1106	8	-0.20971558841940663
1106	9	-0.2477098504195041
1106	10-11	-0.1538167188065458
1106	12-13	-0.165153164948471
1106	14-15	-0.2074901409462271
1106	16-17	-0.1473625859761114
1106	18-19	-0.219930721721866
1106	20-21	-0.2849714924094826
1106	22-23	-0.16197668181837344
1106	24-25	-0.21914087868795917
1106	26-27	-0.3020008002052421
1106	28-29	-0.28468488174814155
1106	30-31	-0.1803462540200087
1106	32-33	-0.24940239906606365
1106	34-35	-0.19147312985629128
1106	36-37	-0.2523248233547619
1106	38-39	-0.19892252677845335
1106	40-41	-0.23066099227232684
1106	42-43	-0.2126638177261242
1106	44-45	-0.2795365578420288
1106	46-47	-0.24686456599132356
1106	48-49	-0.2787676795046039
1106	50-51	-0.2788433746387682
1106	52-53	-0.3375165570987946
1106	54-55	-0.3266006288652008
1106	56-57	-0.42637683308060303
1106	58-59	-0.5286810890355511
1106	60-61	-0.485991450562544
1106	62-63	-0.46026009964779746
1106	64-65	-0.4523375467087618
1106	66-67	-0.3415344042062429
1106	68-69	-0.3728003743385173
1106	70-71	-0.45479724216195194
1106	72-73	-0.4073121027040827
1106	74-75	-0.40898301814853966
1106	76-77	-0.4810247349793997
1106	78-79	-0.4921956824179716
1106	80-81	-0.46887254968169856
1106	82-83	-0.3988642539932954
1106	84-85	-0.42004604232380416
1106	86-87	-0.42610982643399
1106	88-89	-0.4991898941749042
1106	90-91	-0.4699465667979297
1106	92-93	-0.4531062956980598
1106	94-95	-0.5399706987953792
1106	96-97	-0.4539995416994813
1106	98-99	-0.49415419291723595
1106	100	-0.5684553449440344
1107	1	-0.5911260788681574
1107	2	-0.6425102333455044
1107	3	-0.5579484786899229
1107	4	-0.4441987530151579
1107	5	-0.4641570935110977
1107	6	-0.5945361290794224
1107	7	-0.477480037253855
1107	8	-0.49720864043047186
1107	9	-0.5679299712275494
1107	10-11	-0.5028780037958356
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2216	74-75	0.709977431643118
2216	76-77	0.8077873041180794
2216	78-79	0.8505066539212791
2216	80-81	0.8165665717730093
2216	82-83	0.7744044684810909
2216	84-85	0.8116291069457411
2216	86-87	0.8198348214170927
2216	88-89	0.7578754500858089
2216	90-91	0.7710516842128712
2216	92-93	0.7910613024271278
2216	94-95	0.7497020680334678
2216	96-97	0.7761821307297794
2216	98-99	0.7989662786569731
2216	100	0.7708566017714453
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83273.0
3	16422.0
4	11566.0
5	10217.0
6	6881.0
7	5915.0
8	6641.0
9	6982.0
10	7425.0
11	7922.0
12	9040.0
13	10107.0
14	10747.0
15	11083.0
16	10741.0
17	10479.0
18	10483.0
19	9968.0
20	9936.0
21	10311.0
22	12134.0
23	17622.0
24	27803.0
25	36556.0
26	41108.0
27	32965.0
28	31241.0
29	37690.0
30	45048.0
31	52091.0
32	66664.0
33	88736.0
34	125030.0
35	197211.0
36	322912.0
37	586716.0
38	893540.0
39	442942.0
40	1722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98512874774442	21.801170267013283	13.837637876242773	22.376063108999524
2	14.947911176433385	22.648051826798262	40.01668528953796	22.387351707230394
3	17.348474693696204	28.813446491908458	30.483052570542124	23.355026243853217
4	11.667700740919223	19.096620685500874	39.452502861273416	29.783175712306488
5	11.850597173318377	40.472684016106165	34.909418647278706	12.767300163296758
6	27.6099260690203	40.47223149995336	18.931175282920652	12.986667148105688
7	24.75192736422392	35.204874882685125	23.499294831886537	16.543902921204424
8	23.61938585348506	37.80859415194262	21.257501424616287	17.314518569956032
9	24.820037720851857	18.63893852114886	21.59186327108801	34.949160486911275
10-11	22.140692115433712	29.42916070461149	29.424752632694624	19.005394547260174
12-13	22.923349227805474	27.684394306273614	28.77049901722064	20.62175744870027
14-15	20.916280059177346	29.13306448579825	26.263934944633316	23.686720510391087
16-17	20.157048529855107	32.04279826674361	26.948394042036462	20.85175916136482
18-19	20.193213094801994	30.966900428986328	28.791338733711797	20.048547742499885
20-21	21.96415627821776	30.0223590732975	27.987566056453428	20.02591859203131
22-23	21.45801496169912	30.07383965292565	28.049259331805448	20.41888605356978
24-25	21.078109843132818	30.180594636620317	28.039948537358555	20.70134698288831
26-27	20.797703352852686	30.125380120407886	28.10413926357026	20.97277726316917
28-29	20.794495372542034	30.075889167274205	28.29746435342411	20.832151106759646
30-31	21.092705308020708	30.43084865021659	27.913327162849033	20.563118878913674
32-33	20.669343402385053	30.23635672678791	28.352005189786162	20.74229468104088
34-35	20.922739000594255	29.90311345805219	28.330723113608496	20.843424427745056
36-37	21.19268871047342	30.084229088676768	27.89848574214283	20.82459645870698
38-39	20.979493175299734	30.186688701258507	28.103192061889843	20.73062606155192
40-41	20.81505699209499	29.889575418182496	28.33678286345051	20.958584726272
42-43	20.773456291307728	29.90155729081018	28.357987767292393	20.96699865058969
44-45	20.99950495719911	30.004152942645756	28.18048447988155	20.815857620273583
46-47	21.00765855163809	30.18105464227569	28.02793987924876	20.78334692683746
48-49	20.945132437646528	29.909067136186547	28.1283712761927	21.017429149974227
50-51	20.87997682964902	29.87876941363214	28.14943371812666	21.091820038592175
52-53	20.9167276136318	29.894083929124083	28.138736105634116	21.050452351609998
54-55	21.047685478299176	30.15274170135219	27.970051083416188	20.829521736932442
56-57	20.938891686376543	29.90068810172417	27.88604564847912	21.274374563420164
58-59	20.898276020570215	29.634267681505627	28.200283796615533	21.26717250130862
60-61	21.154830180395763	29.96267388524548	27.877638228417045	21.004857705941713
62-63	20.884080316997867	30.002342370993958	27.96894771277326	21.144629599234914
64-65	20.974569035747056	29.787222519019025	28.08217127480688	21.15603717042704
66-67	20.840234412787783	29.80130457670011	27.962554613747415	21.39590639676469
68-69	21.302323244267562	29.934054440816745	27.77073199059184	20.992890324323852
70-71	21.18049893280107	29.7050776043475	27.93727927154872	21.177144191302705
72-73	21.050543725494446	29.823908998789317	28.062412297486922	21.063134978229318
74-75	21.02856104385486	29.887405558879465	27.957369878824412	21.126663518441262
76-77	21.11471151887426	29.87119473525368	27.818561113369515	21.195532632502545
78-79	21.148142336588368	29.81703265201181	28.009669878275012	21.02515513312481
80-81	21.07481580146655	29.7803489567512	28.008693902968734	21.136141338813513
82-83	21.000968641473563	29.805751124439585	28.134129912304694	21.059150321782155
84-85	20.94940541793022	29.732323342508916	27.97906794267156	21.339203296889306
86-87	21.186721313719893	29.767904490327364	27.889074766972428	21.156299428980322
88-89	20.979093682425948	29.652179251000387	28.038391240266176	21.330335826307486
90-91	20.98084652970485	29.6135393477854	27.968183068322627	21.43743105418712
92-93	21.07284056854698	29.746371360829954	27.8730036466921	21.307784423930965
94-95	20.91195857950422	29.59079869489814	28.05821567879378	21.43902704680386
96-97	20.98439812081813	29.711358916636126	27.94744452808934	21.3567984344564
98-99	20.907940067265297	29.718282565397402	27.88166676145314	21.492110605884164
100	20.930472606198418	29.800678788725055	27.745684744226413	21.52316386085012
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20691.0
1	14835.5
2	7639.5
3	5061.5
4	3216.5
5	2293.5
6	1910.5
7	1925.0
8	2083.5
9	2062.5
10	1692.5
11	1271.5
12	1146.5
13	1209.5
14	1282.5
15	1378.0
16	1557.0
17	1876.0
18	2392.5
19	3170.5
20	4535.0
21	6475.5
22	9630.0
23	14436.5
24	19853.5
25	26024.5
26	33653.0
27	42697.5
28	54444.0
29	65345.5
30	72488.0
31	79896.0
32	87961.0
33	95217.5
34	101484.0
35	107317.0
36	111267.5
37	113507.5
38	114411.0
39	113607.5
40	114086.5
41	113533.0
42	110248.5
43	108646.0
44	106794.0
45	102782.5
46	100227.5
47	98287.5
48	95478.0
49	92858.0
50	91373.0
51	87951.0
52	81466.5
53	76120.0
54	72885.0
55	70488.5
56	65141.5
57	58157.0
58	53407.5
59	48503.0
60	42613.0
61	36309.0
62	30551.0
63	25204.0
64	20104.0
65	16258.5
66	13364.0
67	10666.0
68	8256.0
69	6330.0
70	4930.0
71	4004.0
72	3243.0
73	2600.0
74	2230.5
75	1832.5
76	1313.0
77	1079.0
78	905.0
79	721.5
80	523.0
81	350.0
82	262.5
83	188.0
84	143.0
85	128.0
86	116.5
87	99.5
88	86.5
89	64.0
90	51.0
91	45.5
92	38.0
93	29.5
94	26.0
95	27.0
96	25.0
97	20.0
98	17.0
99	24.0
100	35.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17357864258073827
2	0.1677756496796327
3	0.1817569538196021
4	0.1704817085454332
5	0.184823820534176
6	0.07516830182779242
7	0.07664160054361716
8	0.0651859513450616
9	0.05950322772688048
10-11	0.07280801715039974
12-13	0.07178572824554177
14-15	0.06918490500230015
16-17	0.06566702847675947
18-19	0.07028236220898593
20-21	0.07177069458517621
22-23	0.06966598213399802
24-25	0.06897443375718233
26-27	0.07265768054674417
28-29	0.0703274631900826
30-31	0.075829782883877
32-33	0.07273284884857195
34-35	0.07201123315102514
36-37	0.08388782483981636
38-39	0.07849074076858086
40-41	0.07428131586622447
42-43	0.07716777865641171
44-45	0.08794691313851714
46-47	0.07791946167468963
48-49	0.08295573789715172
50-51	0.07990390484294335
52-53	0.07894175057954761
54-55	0.08052028491793124
56-57	0.07371003677233326
58-59	0.08164780944534814
60-61	0.07797959631615187
62-63	0.06959081383217022
64-65	0.07280801715039974
66-67	0.07265768054674417
68-69	0.07128961745347834
70-71	0.06662918274015521
72-73	0.06416366244020362
74-75	0.06929014062485907
76-77	0.0745970227339012
78-79	0.07076343934068378
80-81	0.07882148129662314
82-83	0.07994900582404002
84-85	0.08230929050143271
86-87	0.07831033684419415
88-89	0.0800993424276956
90-91	0.07841557246675306
92-93	0.0694555108888802
94-95	0.0690947030401068
96-97	0.06903456839864457
98-99	0.0785057744289464
100	0.08695469155439027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3325870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.39275882196188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07156239016626	52.4977917144602
2	13.502970239470338	17.929988929785658
3	3.768146009237392	7.505328275917089
4	1.4278822916612557	3.792041784656639
5	0.6704615567946566	2.225689621983236
6	0.3809043589247516	1.5173574741795017
7	0.23900779733903865	1.1107870931709394
8	0.17007689803197867	0.9033499577779649
9	0.1190251386685272	0.7112166592833085
>10	0.594459244871111	7.033750437498643
>50	0.03798841826610394	1.7139007448595245
>100	0.016564389293844496	1.8554710808768458
>500	6.794766257942843E-4	0.3043277626754185
>1k	2.2649220750013834E-4	0.229591672206196
>5k	0.0	0.0
>10k+	4.529844150002766E-5	0.6694067906687857
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22258	0.6692384248332015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.006732073111697E-5	0.0	0.0	0.0
5	0.0	3.006732073111697E-5	0.0	0.0	0.0
6	0.0	3.006732073111697E-5	0.0	0.0	0.0
7	0.0	3.006732073111697E-5	0.0	0.0	0.0
8	0.0	3.006732073111697E-5	0.0	0.0	0.0
9	0.0	3.006732073111697E-5	0.0	9.020196219335091E-5	0.0
10-11	0.0	3.006732073111697E-5	0.0	9.020196219335091E-5	0.0
12-13	0.0	3.006732073111697E-5	0.0	1.0523562255890939E-4	0.0
14-15	0.0	3.006732073111697E-5	0.0	1.2026928292446788E-4	0.0
16-17	0.0	3.006732073111697E-5	0.0	1.3530294329002637E-4	0.0
18-19	0.0	3.006732073111697E-5	0.0	1.6537026402114335E-4	0.0
20-21	0.0	3.006732073111697E-5	0.0	1.8040392438670183E-4	0.0
22-23	0.0	3.006732073111697E-5	0.0	4.810771316978716E-4	0.0
24-25	0.0	6.013464146223394E-5	0.0	8.268513201057166E-4	0.0
26-27	0.0	6.013464146223394E-5	0.0	0.0012177264896102373	0.0
28-29	0.0	6.013464146223394E-5	0.0	0.002224981734102656	0.0
30-31	0.0	6.013464146223394E-5	0.0	0.005818026561471134	0.0
32-33	0.0	6.013464146223394E-5	0.0	0.012823712291821387	0.0
34-35	0.0	6.013464146223394E-5	0.0	0.023587813113561264	0.0
36-37	0.0	6.013464146223394E-5	0.0	0.04123733038272692	0.0
38-39	0.0	6.013464146223394E-5	0.0	0.06542648991091053	0.0
40-41	0.0	6.013464146223394E-5	0.0	0.09072814030614545	0.0
42-43	0.0	6.013464146223394E-5	0.0	0.1166762380970994	0.0
44-45	0.0	6.013464146223394E-5	0.0	0.14975029090132808	0.0
46-47	0.0	6.013464146223394E-5	0.0	0.19067191441637826	0.0
48-49	0.0	6.013464146223394E-5	0.0	0.23419436117467007	0.0
50-51	0.0	9.020196219335091E-5	0.0	0.2793705105731733	0.0
52-53	0.0	9.020196219335091E-5	0.0	0.3235694720479153	0.0
54-55	0.0	9.020196219335091E-5	0.0	0.37662325947797115	0.0
56-57	0.0	9.020196219335091E-5	0.0	0.4394940271267368	0.0
58-59	0.0	9.020196219335091E-5	0.0	0.5114150583155685	0.0
60-61	0.0	9.020196219335091E-5	0.0	0.5885377359908837	0.0
62-63	0.0	1.2026928292446788E-4	0.0	0.6664872649863043	0.0
64-65	0.0	1.2026928292446788E-4	0.0	0.754313908841897	0.0
66-67	0.0	1.2026928292446788E-4	0.0	0.8553701738191811	0.0
68-69	0.0	1.2026928292446788E-4	0.0	0.9736249462546642	0.0
70-71	0.0	1.2026928292446788E-4	0.0	1.104718464642334	0.0
72-73	0.0	1.2026928292446788E-4	0.0	1.2444563377402003	0.0
74-75	0.0	1.2026928292446788E-4	0.0	1.3882081981556706	0.0
76-77	0.0	1.2026928292446788E-4	0.0	1.542363351544107	0.0
78-79	0.0	1.2026928292446788E-4	0.0	1.7259093109472108	0.0
80-81	0.0	1.2026928292446788E-4	0.0	1.9328025448980268	0.0
82-83	0.0	1.2026928292446788E-4	0.0	2.1529705009516307	0.0
84-85	0.0	1.2026928292446788E-4	0.0	2.3790917865099956	0.0
86-87	0.0	1.2026928292446788E-4	0.0	2.6151503215699954	0.0
88	0.0	1.2026928292446788E-4	0.0	2.8138201433008505	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4405	0.0	43.79561	1
GTATCAA	7110	0.0	35.472176	1
ATCAACG	8125	0.0	29.879938	3
TCAACGC	8135	0.0	29.784027	4
GTACATG	15970	0.0	28.28518	1
CAACGCA	8730	0.0	27.861855	5
TATCAAC	8985	0.0	27.595985	2
TACATGG	16195	0.0	26.960032	2
AACGCAG	9220	0.0	26.249414	6
CATGGGT	7735	0.0	25.850105	4
ACATGGG	16415	0.0	25.566856	3
TACCTGG	2300	0.0	23.52466	2
CGCAGAG	10295	0.0	23.32622	8
ACGCAGA	10445	0.0	22.991234	7
ATGGGTA	3460	0.0	21.348003	5
ATGGGTG	4365	0.0	20.802082	5
CTGTCGC	715	0.0	20.37598	9
GTACCTG	2740	0.0	20.092218	1
GCAGAGT	11765	0.0	19.813108	9
CATGGGG	7020	0.0	19.100357	4
>>END_MODULE