FastQCFastQC Report
Wed 25 May 2016
SRR2431381_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431381_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3325870
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230710.6936831565875996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180080.5414523117259544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130160.3913562466362185No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74640.2244224819370571No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42070.1264932183158091No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41630.12517025620363995No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36420.10950518210272801No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35550.10688932519912082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82500.061.898811
GTATCAA164200.046.5357361
ATCAACG215100.035.148463
TCAACGC216900.034.921284
TATCAAC219600.034.57862
CAACGCA223800.033.8866355
AACGCAG231450.032.814716
CGCAGAG260700.029.0067738
ACGCAGA261650.028.9733037
GCAGAGT283100.026.5622279
GTACATG154400.026.3436091
TACATGG155000.025.6325742
ACATGGG154950.024.7604923
CATGGGT74600.024.6429164
TGGTATC27950.024.560562
GTGGTAT29700.024.3820761
GAGTACT200300.022.41992412-13
AGAGTAC272600.022.39585910-11
CAGAGTA281600.022.13905110-11
AGTACTT205950.021.58806412-13
Produced by FastQC (version 0.11.5)