FastQCFastQC Report
Wed 25 May 2016
SRR2431380_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431380_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922748
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA147590.3762413491766486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46450.047.1517331
GTATCAA80450.039.0838661
TCAACGC91850.033.0560384
ATCAACG92350.032.8261763
TATCAAC98750.031.0794682
CAACGCA106300.028.6067475
AACGCAG112550.027.0181876
GTACATG201550.026.7005461
TACATGG206350.025.3961472
ACGCAGA120300.025.2776157
CGCAGAG121550.024.824338
ACATGGG208650.024.485483
CATGGGT61400.022.2752234
GCAGAGT139500.021.2257869
CATGGGG128600.020.210714
GTGGTAT22100.018.7149141
CAGAGTA133050.018.35135310-11
TAGACCG1550.001033936518.1935315
CATGGGA47950.018.1334534
AGAGTAC125650.017.71148310-11
Produced by FastQC (version 0.11.5)