##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431380_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3922748
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174534535483797	33.0	31.0	34.0	28.0	34.0
2	31.237993875721816	34.0	31.0	34.0	28.0	34.0
3	31.330378601939252	34.0	31.0	34.0	28.0	34.0
4	34.57391272648664	37.0	35.0	37.0	32.0	37.0
5	34.67495413929215	37.0	35.0	37.0	32.0	37.0
6	34.75825849633981	37.0	35.0	37.0	32.0	37.0
7	34.657389921554994	37.0	35.0	37.0	32.0	37.0
8	34.64105596382944	37.0	35.0	37.0	32.0	37.0
9	36.17985198131514	39.0	37.0	39.0	32.0	39.0
10-11	36.178378142057554	39.0	37.0	39.0	32.0	39.0
12-13	36.1195092063013	39.0	37.0	39.0	32.0	39.0
14-15	37.499490408254616	41.0	38.0	41.0	32.0	41.0
16-17	37.497183989387025	41.0	38.0	41.0	32.0	41.0
18-19	37.51111172575959	41.0	38.0	41.0	32.0	41.0
20-21	37.562870084950646	41.0	38.0	41.0	32.0	41.0
22-23	37.54013908107275	41.0	38.0	41.0	31.0	41.0
24-25	37.53963892149075	41.0	38.0	41.0	31.0	41.0
26-27	37.53322823694002	41.0	38.0	41.0	31.0	41.0
28-29	37.49626792238502	40.0	38.0	41.0	31.0	41.0
30-31	37.42648329691329	40.0	38.0	41.0	31.0	41.0
32-33	37.367026507948	40.0	38.0	41.0	30.0	41.0
34-35	37.29993157857706	40.0	38.0	41.0	30.0	41.0
36-37	37.19571025209878	40.0	38.0	41.0	30.0	41.0
38-39	37.09061708781701	40.0	38.0	41.0	30.0	41.0
40-41	36.992945506568354	40.0	38.0	41.0	30.0	41.0
42-43	36.85593976467517	40.0	37.0	41.0	30.0	41.0
44-45	36.69724195895327	40.0	37.0	41.0	30.0	41.0
46-47	36.55367372566374	40.0	37.0	41.0	29.5	41.0
48-49	36.410239199663096	40.0	36.0	41.0	29.0	41.0
50-51	36.24805582719053	40.0	36.0	41.0	29.0	41.0
52-53	36.05836278547589	40.0	35.0	41.0	29.0	41.0
54-55	35.87795061013351	39.0	35.0	41.0	28.0	41.0
56-57	35.66412002504367	39.0	35.0	41.0	28.0	41.0
58-59	35.42457086205894	39.0	35.0	41.0	28.0	41.0
60-61	35.24403033281771	39.0	35.0	41.0	28.0	41.0
62-63	35.07988634498061	38.0	34.5	40.5	28.0	41.0
64-65	34.82552180257309	38.0	34.0	40.0	27.0	41.0
66-67	34.52492614871004	37.0	34.0	40.0	26.5	41.0
68-69	34.22385671983008	37.0	34.0	40.0	26.0	41.0
70-71	33.98799515033849	36.0	34.0	39.0	26.0	41.0
72-73	33.75265171252398	36.0	34.0	39.0	26.0	41.0
74-75	33.41464656919078	35.5	33.5	39.0	26.0	41.0
76-77	33.073178802207025	35.0	33.0	37.5	26.0	40.0
78-79	32.73945700819935	35.0	33.0	37.0	25.0	39.0
80-81	32.41061916289296	35.0	33.0	37.0	25.0	39.0
82-83	32.08833297474118	35.0	33.0	36.0	24.5	39.0
84-85	31.80297243157093	35.0	33.0	36.0	24.0	37.0
86-87	31.538732286652113	35.0	33.0	35.5	23.5	37.0
88-89	31.291187580746968	35.0	32.0	35.0	23.0	37.0
90-91	31.082652135696712	35.0	32.0	35.0	21.5	36.0
92-93	30.87766841000238	35.0	32.0	35.0	20.0	36.0
94-95	30.661773965597586	35.0	32.0	35.0	19.0	36.0
96-97	30.474864686694126	35.0	32.0	35.0	17.5	35.5
98-99	30.35716250444841	35.0	32.0	35.0	6.5	35.0
100	30.31615668403884	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.270842412539249
1101	2	0.2864524207862722
1101	3	0.3057571867439002
1101	4	0.2837483100429239
1101	5	0.19092962724003826
1101	6	0.2961716982283491
1101	7	0.35912589454820676
1101	8	0.3155506814314819
1101	9	0.34959539827556796
1101	10-11	0.3418651090955791
1101	12-13	0.3357667374413893
1101	14-15	0.4290670604100768
1101	16-17	0.3985878160656213
1101	18-19	0.3556259152280248
1101	20-21	0.3569467381575393
1101	22-23	0.4175260728889256
1101	24-25	0.34744843841207995
1101	26-27	0.300682089183006
1101	28-29	0.3137993463361255
1101	30-31	0.3712511092254047
1101	32-33	0.40271882736444553
1101	34-35	0.38000692348451537
1101	36-37	0.39103725644234544
1101	38-39	0.3112606566537508
1101	40-41	0.37179576913976575
1101	42-43	0.35421161425961856
1101	44-45	0.38567228506020257
1101	46-47	0.3517291003863008
1101	48-49	0.346365677172912
1101	50-51	0.33686962279445254
1101	52-53	0.36837615156125736
1101	54-55	0.3732340125080924
1101	56-57	0.35034222383860225
1101	58-59	0.3813104207046436
1101	60-61	0.3796133354564688
1101	62-63	0.30751261098721017
1101	64-65	0.28063174333326657
1101	66-67	0.29665392253735945
1101	68-69	0.24437896054738673
1101	70-71	0.2694261126465918
1101	72-73	0.23370959534645408
1101	74-75	0.28143824505947634
1101	76-77	0.31432191302894097
1101	78-79	0.23814903443150826
1101	80-81	0.2262875625338694
1101	82-83	0.22308791783996895
1101	84-85	0.2613332836142206
1101	86-87	0.30362892631046634
1101	88-89	0.2706552557769726
1101	90-91	0.2460648374653509
1101	92-93	0.2704512030432191
1101	94-95	0.3267974464263972
1101	96-97	0.21134975442441473
1101	98-99	0.2830130288520536
1101	100	0.3218204144887835
1102	1	0.1859147716670151
1102	2	0.29038307705906874
1102	3	0.33945886930645486
1102	4	0.26369117771432826
1102	5	0.33037664901393526
1102	6	0.3278716022088872
1102	7	0.35558770471728707
1102	8	0.3573617392983053
1102	9	0.41740561029232737
1102	10-11	0.4117573905389591
1102	12-13	0.41731667030765607
1102	14-15	0.4629519439109515
1102	16-17	0.4783080863581617
1102	18-19	0.3701430791436806
1102	20-21	0.4134261044590488
1102	22-23	0.38522483641990135
1102	24-25	0.38388784903334283
1102	26-27	0.4047773217143771
1102	28-29	0.430079413584707
1102	30-31	0.4130752493069636
1102	32-33	0.4120374087530507
1102	34-35	0.41222297576143774
1102	36-37	0.45534448077086864
1102	38-39	0.43110467870577196
1102	40-41	0.4404215723602789
1102	42-43	0.3845547128171347
1102	44-45	0.4107626746755173
1102	46-47	0.3986173058709426
1102	48-49	0.3294167192811628
1102	50-51	0.3181813794612651
1102	52-53	0.2982497384199192
1102	54-55	0.3905290499047567
1102	56-57	0.360405642620762
1102	58-59	0.43582016470767115
1102	60-61	0.43393208911088266
1102	62-63	0.506905186780422
1102	64-65	0.5230964126153808
1102	66-67	0.4952689054680661
1102	68-69	0.48732497544013853
1102	70-71	0.43719992414985853
1102	72-73	0.46246085800487435
1102	74-75	0.49613037111410563
1102	76-77	0.38330518027711946
1102	78-79	0.34955028443213365
1102	80-81	0.3533613057799201
1102	82-83	0.35994216449164895
1102	84-85	0.3204569549829941
1102	86-87	0.3508062886863179
1102	88-89	0.2754385528845589
1102	90-91	0.32327671791388113
1102	92-93	0.26934610542343407
1102	94-95	0.30190513752035386
1102	96-97	0.33976013051811904
1102	98-99	0.31513016560463925
1102	100	0.28056809701438823
1103	1	0.0842830941331627
1103	2	0.15405173009437334
1103	3	0.13590309714564697
1103	4	0.15873953237588978
1103	5	0.12068405617520028
1103	6	0.11610402949228416
1103	7	0.1169576344928771
1103	8	0.11932684123815562
1103	9	0.24383292815315372
1103	10-11	0.22801779928291666
1103	12-13	0.10171346159619077
1103	14-15	0.17952938398144624
1103	16-17	0.19361223213349632
1103	18-19	0.2042239467461826
1103	20-21	0.1799694538162555
1103	22-23	0.2210685754204178
1103	24-25	0.2311269935410465
1103	26-27	0.32365126976357317
1103	28-29	0.2790902906144552
1103	30-31	0.23591110436851892
1103	32-33	0.1294438562053486
1103	34-35	0.23935746054861795
1103	36-37	0.2216751631512679
1103	38-39	0.2125736069282098
1103	40-41	0.1722069165534208
1103	42-43	0.11182236060714246
1103	44-45	0.053526705403925234
1103	46-47	0.029756526436628405
1103	48-49	0.03586540229660784
1103	50-51	-0.1063535961337223
1103	52-53	-0.09937671105075196
1103	54-55	-0.07276374378612616
1103	56-57	-0.16521358995666446
1103	58-59	-0.08003804746209653
1103	60-61	-0.08503613145234112
1103	62-63	-0.08720216573724571
1103	64-65	-0.058957595935609675
1103	66-67	-0.032617051865628355
1103	68-69	0.04558563524928161
1103	70-71	-0.0038861542858583675
1103	72-73	-0.034197267522792174
1103	74-75	-0.011776705231859808
1103	76-77	-0.0253509071920206
1103	78-79	-0.13286653791956127
1103	80-81	-0.08601974470633422
1103	82-83	-0.046266775890217104
1103	84-85	-0.08700263459927626
1103	86-87	-0.03676746171058909
1103	88-89	-0.05607652702555299
1103	90-91	-0.08288348628579811
1103	92-93	-0.045833492330768166
1103	94-95	-0.07986285363821466
1103	96-97	-0.06482033218024341
1103	98-99	-0.048729001597390464
1103	100	-0.09128306563892608
1104	1	0.06389501194084346
1104	2	0.046943885216798265
1104	3	0.13165762470547548
1104	4	0.0946104293672505
1104	5	0.0345241290737448
1104	6	0.10988398475908667
1104	7	0.1080777395803878
1104	8	0.14009771968930096
1104	9	0.1642501224269921
1104	10-11	0.16456845154344535
1104	12-13	0.18872844761213514
1104	14-15	0.19803985525791745
1104	16-17	0.20081672281013851
1104	18-19	0.17937319547302621
1104	20-21	0.13441496333685166
1104	22-23	0.18543980574116148
1104	24-25	0.18559443574094558
1104	26-27	0.2119552346081761
1104	28-29	0.2290927757921466
1104	30-31	0.20915804819166084
1104	32-33	0.24179479485302124
1104	34-35	0.20355725305599748
1104	36-37	0.2476057438544501
1104	38-39	0.24795890808835708
1104	40-41	0.21218293261634358
1104	42-43	0.20478070824011496
1104	44-45	0.17261862936538108
1104	46-47	0.14154731392237352
1104	48-49	0.07967908497492004
1104	50-51	0.08716979412201908
1104	52-53	0.07312759856294093
1104	54-55	0.05345985120091967
1104	56-57	0.10537484489420734
1104	58-59	0.019718010651615714
1104	60-61	0.0740333864626308
1104	62-63	0.02885847259840091
1104	64-65	0.08625701690920806
1104	66-67	0.13292986473891233
1104	68-69	0.08993927208987174
1104	70-71	0.0680557162245421
1104	72-73	0.08528123963490941
1104	74-75	0.10956008368187753
1104	76-77	0.04458309753376
1104	78-79	0.09552428980239114
1104	80-81	0.08503929601508275
1104	82-83	0.05659062619181299
1104	84-85	0.028762385933024603
1104	86-87	0.05698645836136507
1104	88-89	0.023409948479667264
1104	90-91	0.023647718473117862
1104	92-93	0.024663740443596538
1104	94-95	0.056671467454634694
1104	96-97	-0.0012449898766000445
1104	98-99	-0.022432034697590808
1104	100	-0.03182294090667526
1105	1	0.050268817396194265
1105	2	0.1274669533770414
1105	3	0.15370876070952733
1105	4	0.15627203306321746
1105	5	0.1147381978035753
1105	6	0.1763196533876581
1105	7	0.08594844396618839
1105	8	0.14481435154804245
1105	9	0.2030283643104127
1105	10-11	0.16017236901702603
1105	12-13	0.16741959057776512
1105	14-15	0.2541889122796306
1105	16-17	0.3095316775598391
1105	18-19	0.23886025325417393
1105	20-21	0.24955087245652408
1105	22-23	0.16125700085539307
1105	24-25	0.1688940686755629
1105	26-27	0.17316204113940614
1105	28-29	0.19849202267525357
1105	30-31	0.19757674004660686
1105	32-33	0.20006681877556076
1105	34-35	0.2067600566760177
1105	36-37	0.2702575538664789
1105	38-39	0.2534262835709171
1105	40-41	0.24566902526871104
1105	42-43	0.24523645471185063
1105	44-45	0.20090750348726516
1105	46-47	0.2544013263266507
1105	48-49	0.20778569215167408
1105	50-51	0.19845301986721609
1105	52-53	0.2405176371680895
1105	54-55	0.22351586031426507
1105	56-57	0.24597607266460386
1105	58-59	0.21577712083450962
1105	60-61	0.256578003891903
1105	62-63	0.29717835741581666
1105	64-65	0.30623328423406093
1105	66-67	0.24578079189542024
1105	68-69	0.19665221747307982
1105	70-71	0.24386555420948497
1105	72-73	0.2783120604690339
1105	74-75	0.23482913310913034
1105	76-77	0.19685486830776
1105	78-79	0.20107409677663668
1105	80-81	0.15295604179065947
1105	82-83	0.23794338867519826
1105	84-85	0.21137282198137086
1105	86-87	0.2218007897327965
1105	88-89	0.26389543990426034
1105	90-91	0.20356686915978273
1105	92-93	0.2413552473102527
1105	94-95	0.2580114934023605
1105	96-97	0.2744654873083867
1105	98-99	0.3380957008887684
1105	100	0.3046445295065894
1106	1	0.07988235192754445
1106	2	0.09321528389207501
1106	3	0.08614358153247181
1106	4	0.009209514553191411
1106	5	-0.005346852782537326
1106	6	0.0324191250756769
1106	7	0.04455596453023247
1106	8	0.010654754517290144
1106	9	0.10619895271430124
1106	10-11	0.03615459461483539
1106	12-13	0.10329614183740432
1106	14-15	0.06764982149213239
1106	16-17	0.07076499465211583
1106	18-19	0.10399261785631353
1106	20-21	0.08919636365291694
1106	22-23	0.1475322342011438
1106	24-25	0.14807076658238572
1106	26-27	0.19006951727938315
1106	28-29	0.22019664086715096
1106	30-31	0.2274495940777328
1106	32-33	0.16363353368770817
1106	34-35	0.1674642258505017
1106	36-37	0.1763261556071356
1106	38-39	0.1512012589704952
1106	40-41	0.2154690811570461
1106	42-43	0.11419973798452787
1106	44-45	0.1428269812895664
1106	46-47	0.15538337279517833
1106	48-49	0.12176959917641739
1106	50-51	0.035375327912277044
1106	52-53	0.08042931448698454
1106	54-55	0.0804388490506156
1106	56-57	0.11239267484472748
1106	58-59	0.0470433021802279
1106	60-61	-0.009195046464917311
1106	62-63	0.07867346599204694
1106	64-65	0.09097648448627638
1106	66-67	0.09500325136443166
1106	68-69	0.12577619165934806
1106	70-71	0.1594827023756764
1106	72-73	0.04462667837432832
1106	74-75	0.0734921001588944
1106	76-77	0.07060663571674297
1106	78-79	0.028007140637036798
1106	80-81	-0.025022161757533468
1106	82-83	1.485418665652105E-4
1106	84-85	-2.3965210458598563E-4
1106	86-87	0.022223236304498073
1106	88-89	0.042047816830500295
1106	90-91	-0.04198343989795106
1106	92-93	0.0027522885720863144
1106	94-95	-0.06390360170335185
1106	96-97	-0.012070020893347078
1106	98-99	0.049066354734552675
1106	100	0.07416042663383493
1107	1	0.021193925422654303
1107	2	0.06475615837876347
1107	3	0.02178741926942962
1107	4	0.07161657055700488
1107	5	0.043753340627944226
1107	6	0.05109260662079862
1107	7	0.054263231163616865
1107	8	0.07574098915272032
1107	9	0.06418902738257515
1107	10-11	0.06352334029919149
1107	12-13	0.07707457509079063
1107	14-15	0.1047350195030674
1107	16-17	0.07396563802024048
1107	18-19	0.07539494600015217
1107	20-21	0.0847813607743717
1107	22-23	0.11625967761727196
1107	24-25	0.15687038889276295
1107	26-27	0.2159620296675726
1107	28-29	0.13567989311998474
1107	30-31	0.2185013700798777
1107	32-33	0.2734170252686212
1107	34-35	0.22988106543286335
1107	36-37	0.22259900352423045
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2216	52-53	-0.6671858936168533
2216	54-55	-0.7734147641386073
2216	56-57	-0.7199920967741775
2216	58-59	-0.6602381014201342
2216	60-61	-0.5338114280699671
2216	62-63	-0.6486166771138997
2216	64-65	-0.6911338249965624
2216	66-67	-0.7502634888165716
2216	68-69	-0.7601996208694999
2216	70-71	-0.8724041423180893
2216	72-73	-0.7751579327222871
2216	74-75	-0.7927867359697558
2216	76-77	-0.7463352396583929
2216	78-79	-0.6897986296118503
2216	80-81	-0.5810946342136205
2216	82-83	-0.57745576996696
2216	84-85	-0.5577528710445918
2216	86-87	-0.587171464911691
2216	88-89	-0.6205674041770521
2216	90-91	-0.5401265945110687
2216	92-93	-0.6152382260872749
2216	94-95	-0.5834782692666423
2216	96-97	-0.669177519208489
2216	98-99	-0.7554488229902354
2216	100	-0.7074031798602043
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76647.0
3	15852.0
4	11386.0
5	9987.0
6	7196.0
7	6095.0
8	6374.0
9	6773.0
10	6935.0
11	7438.0
12	8322.0
13	8959.0
14	9437.0
15	9402.0
16	9486.0
17	9672.0
18	9854.0
19	9566.0
20	9698.0
21	10587.0
22	12547.0
23	18677.0
24	29165.0
25	37415.0
26	39454.0
27	32465.0
28	33199.0
29	39741.0
30	48154.0
31	57816.0
32	74665.0
33	100942.0
34	146695.0
35	232785.0
36	422971.0
37	769199.0
38	1083858.0
39	491996.0
40	1338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.00119763501499	21.101370448292272	14.181752457662785	22.715679459029946
2	14.94974307518999	22.193730254569317	39.518851986989716	23.33767468325098
3	17.431921414233805	28.320033976199326	30.395161960524593	23.85288264904228
4	12.223866762653897	19.116261474110978	38.56435699092268	30.09551477231244
5	12.704270276112844	40.19691318118134	34.30081086096875	12.79800568173707
6	28.38938417660273	39.592907828899534	18.83350651125181	13.184201483245928
7	25.41929790034945	34.81872911540583	22.86517002876555	16.89680295547917
8	24.661308857974053	36.71987086603575	20.780483477399006	17.838336798591193
9	25.656988417303378	17.983056775505336	20.94182445571319	35.4181303514781
10-11	23.180306254696962	28.506406733239047	28.88675234809883	19.42653466396516
12-13	23.87796768999691	26.54342058169426	28.398153539304587	21.180458189004238
14-15	21.762766815507902	27.885362506079918	25.71435891370029	24.63751176471188
16-17	21.27651330138974	30.81673867401118	26.45970375869161	21.447044265907472
18-19	21.403133721564576	29.97340129929325	27.858774002306546	20.764690976835627
20-21	22.996697723126747	28.76491174044318	27.20201501600409	21.036375520425988
22-23	22.7624996558535	28.921766068072685	27.03474707016612	21.280987205907696
24-25	22.276348111069076	29.013474737607414	27.10629130395325	21.60388584737026
26-27	22.024534841391798	29.058736375622395	27.20684581319014	21.709882969795665
28-29	21.891694291858666	29.00499853674006	27.47619780827114	21.62710936313013
30-31	22.07168291208102	29.026246396658667	27.30877690843256	21.593293782827754
32-33	21.972734419850575	29.107299270817293	27.37854942504591	21.541416884286217
34-35	22.23508876940349	28.84461352092972	27.2455814138456	21.674716295821195
36-37	22.12533152779633	28.870386270033148	27.24732763868594	21.75695456348458
38-39	21.995180419440658	28.914411529876503	27.29776422038836	21.792643830294477
40-41	22.059854469366883	28.680277193436847	27.412989567517464	21.846878769678806
42-43	21.974671837191686	28.83942583107556	27.466421498398574	21.719480833334185
44-45	22.091770870828306	28.77883055449904	27.361074430475778	21.768324144196875
46-47	22.056910104855064	28.729885274302607	27.364554133989742	21.848650486852584
48-49	21.985875717736647	28.666944703050007	27.32974435268337	22.017435226529976
50-51	21.919735858637875	28.70352620153015	27.351489313103976	22.025248626727997
52-53	21.982204821721915	28.715482105911466	27.38641380991081	21.91589926245581
54-55	21.97528365319414	28.696821717836578	27.387293295414338	21.940601333554945
56-57	21.911922458439847	28.64165630828185	27.35196092127254	22.09446031200577
58-59	22.074232145424585	28.508191196579542	27.410300126340005	22.00727653165587
60-61	22.090942369991648	28.708497206550103	27.241668340663228	21.95889208279502
62-63	21.897328097547945	28.67176275406934	27.471774888420057	21.95913425996266
64-65	22.122530190892988	28.579292957295877	27.321360857409253	21.976815994401882
66-67	21.908531978092906	28.610593899990516	27.282774728328203	22.19809939358837
68-69	22.04418943047068	28.648768669310392	27.35168050560474	21.955361394614183
70-71	22.03698784627511	28.527998739659033	27.344631875409792	22.090381538656064
72-73	22.048739811988945	28.62045943303011	27.399567853963596	21.931232901017346
74-75	22.01405749234975	28.571412183499934	27.353949323280517	22.060581000869796
76-77	22.000355363128094	28.442038591314052	27.23453048730125	22.323075558256612
78-79	21.940193456219976	28.45723202204169	27.502072526708314	22.100501995030015
80-81	21.80140322567282	28.62340744592916	27.495320562947274	22.079868765450748
82-83	21.8563909593306	28.551480782376505	27.596956770400695	21.9951714878922
84-85	21.922876169390225	28.457453283342478	27.37475997188174	22.24491057538556
86-87	22.057487037604012	28.509750826791695	27.350980563607706	22.081781571996583
88-89	21.9443677524469	28.469909246811504	27.41155968069276	22.174163320048834
90-91	21.936048665197077	28.433957343512873	27.333613926746764	22.296380064543282
92-93	21.885517808376324	28.488316290274778	27.383734899446488	22.242431001902407
94-95	21.884788084407553	28.384245021584213	27.512680494618635	22.218286399389598
96-97	21.948229073856677	28.529762291707293	27.28024187637741	22.241766758058613
98-99	21.794189856090522	28.53748094873363	27.40411144077859	22.26421775439726
100	21.81986390229191	28.492184948693772	27.480922841680172	22.20702830733415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11234.0
1	8632.0
2	4920.5
3	3236.5
4	2319.5
5	1790.0
6	1591.5
7	1655.5
8	1819.0
9	1902.5
10	1606.0
11	1292.5
12	1274.5
13	1316.5
14	1367.0
15	1460.0
16	1703.5
17	2080.5
18	2574.5
19	3354.0
20	4705.0
21	6707.0
22	9978.0
23	14630.5
24	19894.0
25	25473.0
26	32735.5
27	41509.5
28	53056.0
29	65680.0
30	73187.5
31	80254.5
32	88910.0
33	96772.5
34	104004.5
35	109582.5
36	113142.0
37	115825.0
38	117691.0
39	118378.5
40	120310.0
41	121868.0
42	121358.0
43	121414.0
44	121268.0
45	121380.5
46	123407.5
47	122692.5
48	120490.0
49	119754.0
50	119632.5
51	117688.0
52	113437.5
53	109840.0
54	108323.0
55	106627.0
56	97688.5
57	85461.5
58	77435.5
59	72349.0
60	64531.0
61	54517.0
62	47652.5
63	40347.0
64	32198.0
65	26015.0
66	20528.0
67	15894.0
68	12578.0
69	9851.0
70	7539.5
71	5738.0
72	4402.0
73	3376.5
74	2644.5
75	2030.0
76	1541.0
77	1198.0
78	847.0
79	620.5
80	481.5
81	351.5
82	275.5
83	224.0
84	191.0
85	155.0
86	132.5
87	119.5
88	110.0
89	91.5
90	71.0
91	62.5
92	59.0
93	55.5
94	41.5
95	31.0
96	28.0
97	23.5
98	21.0
99	25.0
100	54.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.333696684059236E-4
2	1.2746166717821281E-4
3	5.0984666871285134E-5
4	1.0196933374257027E-4
5	1.0196933374257027E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	1.2746166717821283E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.2746166717821283E-5
82-83	1.911925007673192E-4
84-85	7.775161697870982E-4
86-87	0.001402078338960341
88-89	0.0033394956800691764
90-91	0.0027786643444850396
92-93	0.002179594508747439
94-95	0.0022815638424900095
96-97	0.002115863675158333
98-99	0.0027659181777672185
100	0.0031865416794553203
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3922748.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.98379525054733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.87014971370499	47.309309118068946
2	12.634527156254977	15.157337800565571
3	3.609699571986496	6.495704401260789
4	1.520790421941412	3.6489112515490856
5	0.8597630650633509	2.5785925829371528
6	0.5509012326122551	1.9827088044172596
7	0.38077042497713903	1.598803864650482
8	0.2580794657948368	1.23844686676865
9	0.19712199486334075	1.0641712841335869
>10	1.0136786483242444	10.785440184645331
>50	0.06363839310624583	2.6190262584363504
>100	0.03858203195136	4.081727909934611
>500	0.0017872395841598864	0.7099295495915736
>1k	4.680865156176112E-4	0.3531652296691423
>5k	0.0	0.0
>10k+	4.255331960159918E-5	0.37672489337139975
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14759	0.3762413491766486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	2.5492333435642564E-5	0.0	0.0
4	0.0	0.0	2.5492333435642564E-5	2.5492333435642564E-5	0.0
5	0.0	0.0	2.5492333435642564E-5	2.5492333435642564E-5	0.0
6	0.0	0.0	2.5492333435642564E-5	2.5492333435642564E-5	0.0
7	0.0	0.0	2.5492333435642564E-5	2.5492333435642564E-5	0.0
8	0.0	0.0	5.098466687128513E-5	2.5492333435642564E-5	5.098466687128513E-5
9	0.0	0.0	5.098466687128513E-5	2.5492333435642564E-5	5.098466687128513E-5
10-11	0.0	0.0	5.098466687128513E-5	5.098466687128513E-5	5.098466687128513E-5
12-13	0.0	2.5492333435642564E-5	5.098466687128513E-5	5.098466687128513E-5	5.098466687128513E-5
14-15	2.5492333435642564E-5	5.098466687128513E-5	5.098466687128513E-5	7.64770003069277E-5	5.098466687128513E-5
16-17	2.5492333435642564E-5	5.098466687128513E-5	5.098466687128513E-5	1.0196933374257025E-4	5.098466687128513E-5
18-19	2.5492333435642564E-5	5.098466687128513E-5	5.098466687128513E-5	1.2746166717821281E-4	5.098466687128513E-5
20-21	2.5492333435642564E-5	5.098466687128513E-5	5.098466687128513E-5	2.2943100092078308E-4	6.373083358910641E-5
22-23	2.5492333435642564E-5	5.098466687128513E-5	5.098466687128513E-5	5.353390021484939E-4	7.64770003069277E-5
24-25	2.5492333435642564E-5	5.098466687128513E-5	5.098466687128513E-5	7.647700030692769E-4	7.64770003069277E-5
26-27	2.5492333435642564E-5	7.64770003069277E-5	5.098466687128513E-5	9.687086705544174E-4	7.64770003069277E-5
28-29	2.5492333435642564E-5	1.0196933374257025E-4	5.098466687128513E-5	0.0017079863401880517	7.64770003069277E-5
30-31	2.5492333435642564E-5	1.0196933374257025E-4	5.098466687128513E-5	0.0037983576819107423	7.64770003069277E-5
32-33	5.098466687128513E-5	1.0196933374257025E-4	5.098466687128513E-5	0.007252568862440309	7.64770003069277E-5
34-35	5.098466687128513E-5	1.0196933374257025E-4	5.098466687128513E-5	0.013396221220430167	7.64770003069277E-5
36-37	5.098466687128513E-5	1.0196933374257025E-4	5.098466687128513E-5	0.024319686097603006	7.64770003069277E-5
38-39	7.64770003069277E-5	1.0196933374257025E-4	5.098466687128513E-5	0.03919446265730045	7.64770003069277E-5
40-41	7.64770003069277E-5	1.0196933374257025E-4	5.098466687128513E-5	0.054846755386784976	7.64770003069277E-5
42-43	8.922316702474897E-5	1.0196933374257025E-4	5.098466687128513E-5	0.07028236328206655	7.64770003069277E-5
44-45	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.09002617552797171	7.64770003069277E-5
46-47	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.114753738960545	7.64770003069277E-5
48-49	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.1419413125696578	7.64770003069277E-5
50-51	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.16938380951312704	7.64770003069277E-5
52-53	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.1963929367881903	7.64770003069277E-5
54-55	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.22667782890973368	7.64770003069277E-5
56-57	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.2654261757319104	7.64770003069277E-5
58-59	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.3098975514103889	7.64770003069277E-5
60-61	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.3593654244422533	7.64770003069277E-5
62-63	1.0196933374257025E-4	1.0196933374257025E-4	5.098466687128513E-5	0.40952159047687997	8.922316702474897E-5
64-65	1.0196933374257025E-4	1.1471550046039154E-4	5.098466687128513E-5	0.46320844469234324	1.0196933374257025E-4
66-67	1.0196933374257025E-4	1.2746166717821281E-4	5.098466687128513E-5	0.526340208445712	1.0196933374257025E-4
68-69	1.0196933374257025E-4	1.2746166717821281E-4	5.098466687128513E-5	0.6021799004167487	1.2746166717821281E-4
70-71	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	0.6840740215787504	1.2746166717821281E-4
72-73	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	0.7731187422694499	1.2746166717821281E-4
74-75	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	0.8645342499696641	1.2746166717821281E-4
76-77	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	0.9640563197024127	1.2746166717821281E-4
78-79	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	1.0804033295026854	1.2746166717821281E-4
80-81	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	1.2156656507122048	1.2746166717821281E-4
82-83	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	1.3621955833002783	1.2746166717821281E-4
84-85	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	1.5116061495665793	1.2746166717821281E-4
86-87	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	1.670754787205296	1.2746166717821281E-4
88	1.2746166717821281E-4	1.2746166717821281E-4	5.098466687128513E-5	1.8009568802278402	1.2746166717821281E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4645	0.0	47.151733	1
GTATCAA	8045	0.0	39.083866	1
TCAACGC	9185	0.0	33.056038	4
ATCAACG	9235	0.0	32.826176	3
TATCAAC	9875	0.0	31.079468	2
CAACGCA	10630	0.0	28.606747	5
AACGCAG	11255	0.0	27.018187	6
GTACATG	20155	0.0	26.700546	1
TACATGG	20635	0.0	25.396147	2
ACGCAGA	12030	0.0	25.277615	7
CGCAGAG	12155	0.0	24.82433	8
ACATGGG	20865	0.0	24.48548	3
CATGGGT	6140	0.0	22.275223	4
GCAGAGT	13950	0.0	21.225786	9
CATGGGG	12860	0.0	20.21071	4
GTGGTAT	2210	0.0	18.714914	1
CAGAGTA	13305	0.0	18.351353	10-11
TAGACCG	155	0.0010339365	18.193531	5
CATGGGA	4795	0.0	18.133453	4
AGAGTAC	12565	0.0	17.711483	10-11
>>END_MODULE