FastQCFastQC Report
Wed 25 May 2016
SRR2431380_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431380_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922748
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227590.5801800166617892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173110.44129778410440845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123910.31587550360104705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78150.064.1728741
GTATCAA161700.046.653161
TCAACGC213700.035.0787854
ATCAACG214750.034.9072723
TATCAAC216600.034.674222
CAACGCA224750.033.3118635
AACGCAG233450.032.110696
GTGGTAT23850.031.3346461
ACGCAGA261250.028.6757557
CGCAGAG262400.028.4605278
GCAGAGT286650.025.872479
GTACATG189150.025.7684611
TGGTATC28600.025.3071382
TACATGG194600.024.5138532
ACATGGG199500.023.2756823
TATAACG2905.456968E-1222.6891572
AGAGTAC269200.022.64371910-11
GAGTACT174400.022.52903712-13
AGTACTT179850.022.25138512-13
CAGAGTA280850.021.68769510-11
Produced by FastQC (version 0.11.5)