##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431380_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3922748
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75509668222379	34.0	31.0	34.0	31.0	34.0
2	32.88602352228591	34.0	31.0	34.0	31.0	34.0
3	32.952617654766506	34.0	31.0	34.0	31.0	34.0
4	36.34683543271196	37.0	37.0	37.0	35.0	37.0
5	36.29719140765606	37.0	37.0	37.0	35.0	37.0
6	36.327475025160936	37.0	37.0	37.0	35.0	37.0
7	36.19720461268478	37.0	37.0	37.0	35.0	37.0
8	36.25140666695898	37.0	37.0	37.0	35.0	37.0
9	38.06707032926918	39.0	39.0	39.0	35.0	39.0
10-11	38.05690806546839	39.0	39.0	39.0	35.0	39.0
12-13	38.03449418621844	39.0	39.0	39.0	35.0	39.0
14-15	39.5245352237768	41.0	39.0	41.0	36.0	41.0
16-17	39.534244998659105	41.0	39.5	41.0	36.5	41.0
18-19	39.60521297824892	41.0	40.0	41.0	37.0	41.0
20-21	39.614802811702404	41.0	40.0	41.0	37.0	41.0
22-23	39.553169104923384	41.0	40.0	41.0	36.5	41.0
24-25	39.525116958825805	41.0	40.0	41.0	36.0	41.0
26-27	39.445724272882174	41.0	39.5	41.0	36.0	41.0
28-29	39.366548654157754	41.0	39.0	41.0	36.0	41.0
30-31	39.21983148038059	41.0	39.0	41.0	35.5	41.0
32-33	39.041135831310086	41.0	39.0	41.0	35.0	41.0
34-35	38.95360267853046	41.0	39.0	41.0	35.0	41.0
36-37	38.86072352850604	41.0	38.5	41.0	35.0	41.0
38-39	38.75459945426013	41.0	38.0	41.0	35.0	41.0
40-41	38.6621925497126	41.0	38.0	41.0	35.0	41.0
42-43	38.53929146098602	41.0	38.0	41.0	35.0	41.0
44-45	38.33991477403086	41.0	38.0	41.0	34.5	41.0
46-47	38.06714221764946	40.0	38.0	41.0	34.0	41.0
48-49	37.84314286821381	40.0	38.0	41.0	33.0	41.0
50-51	37.69482758005357	40.0	37.5	41.0	33.0	41.0
52-53	37.563595214375226	40.0	37.0	41.0	33.0	41.0
54-55	37.40641751649609	40.0	37.0	41.0	33.0	41.0
56-57	37.22924235765336	40.0	36.0	41.0	33.0	41.0
58-59	37.02686267381948	40.0	36.0	41.0	32.5	41.0
60-61	36.823387839341194	39.0	35.0	41.0	32.0	41.0
62-63	36.58976717342027	39.0	35.0	41.0	32.0	41.0
64-65	36.315500638837875	39.0	35.0	41.0	31.5	41.0
66-67	36.027918183885376	38.0	35.0	41.0	31.0	41.0
68-69	35.76078440419828	37.0	35.0	40.0	31.0	41.0
70-71	35.51014186993403	37.0	35.0	40.0	31.0	41.0
72-73	35.2550585711853	36.5	35.0	39.0	31.0	41.0
74-75	34.95465614920968	36.0	35.0	39.0	31.0	41.0
76-77	34.63883953289888	35.5	35.0	39.0	31.0	40.5
78-79	34.32537025065082	35.0	35.0	37.0	31.0	39.5
80-81	34.025102428195744	35.0	35.0	37.0	30.5	39.0
82-83	33.71469872650499	35.0	35.0	36.5	30.0	39.0
84-85	33.45409506295077	35.0	34.0	36.0	30.0	37.5
86-87	33.234879604807645	35.0	34.0	36.0	30.0	37.0
88-89	33.019680336335654	35.0	34.0	35.5	30.0	37.0
90-91	32.82644424265847	35.0	34.0	35.0	29.0	36.0
92-93	32.682986008787715	35.0	34.0	35.0	29.0	36.0
94-95	32.55853575095826	35.0	34.0	35.0	29.0	36.0
96-97	32.43358227446678	35.0	34.0	35.0	29.0	36.0
98-99	32.31831613960418	35.0	34.0	35.0	29.0	36.0
100	32.2172163493551	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.17485369973510956
1101	2	0.12841319562045328
1101	3	0.14818214103584637
1101	4	0.09436616652427432
1101	5	0.13683427766777356
1101	6	0.1384794857084799
1101	7	0.17428218112409866
1101	8	0.20711743715603603
1101	9	0.26898545135085783
1101	10-11	0.23947840180233726
1101	12-13	0.24423936419447045
1101	14-15	0.26553496624075734
1101	16-17	0.28992965964782513
1101	18-19	0.29431266900233055
1101	20-21	0.2944308718588573
1101	22-23	0.285140020330239
1101	24-25	0.28456431292077156
1101	26-27	0.26366827828486095
1101	28-29	0.2778563660196127
1101	30-31	0.2849820849955691
1101	32-33	0.28564354948872506
1101	34-35	0.2765255154892685
1101	36-37	0.27153505677208045
1101	38-39	0.22757962158627265
1101	40-41	0.22886055066592093
1101	42-43	0.29503036985549613
1101	44-45	0.31792502963782
1101	46-47	0.3289680977959648
1101	48-49	0.22860419168674184
1101	50-51	0.2835866387895223
1101	52-53	0.20596198160257018
1101	54-55	0.22322913381083254
1101	56-57	0.20514799439203557
1101	58-59	0.10970693794840969
1101	60-61	0.1122541777752204
1101	62-63	0.13617644213461233
1101	64-65	0.22065105101408733
1101	66-67	0.22248025188397946
1101	68-69	0.15596685717213887
1101	70-71	0.14513615774033894
1101	72-73	0.1079964492307397
1101	74-75	0.13468300616931828
1101	76-77	0.0899936307355631
1101	78-79	0.055097798759561556
1101	80-81	0.08616925802272135
1101	82-83	0.13550075051289667
1101	84-85	0.0866279631141893
1101	86-87	0.07821072827319853
1101	88-89	0.14040034003173218
1101	90-91	0.08617364794654492
1101	92-93	0.12475668436815823
1101	94-95	0.15065271759178955
1101	96-97	0.18359435338950192
1101	98-99	0.21877054143233465
1101	100	0.2738794179893347
1102	1	0.12414044440573235
1102	2	0.1465284679077783
1102	3	0.1745891407197746
1102	4	0.10300632023530198
1102	5	0.13440318274133034
1102	6	0.11668241882091479
1102	7	0.1416486464145308
1102	8	0.2037328385178796
1102	9	0.22647494438508176
1102	10-11	0.23055313570159797
1102	12-13	0.2104345218776018
1102	14-15	0.310566246215636
1102	16-17	0.3629243733345646
1102	18-19	0.2947632064966115
1102	20-21	0.27841918974069557
1102	22-23	0.2768008074900976
1102	24-25	0.2946164969765448
1102	26-27	0.3258925344086592
1102	28-29	0.31013733004901667
1102	30-31	0.3101485212255213
1102	32-33	0.35026624493426795
1102	34-35	0.32333008467745117
1102	36-37	0.3377230741624402
1102	38-39	0.35935347503252046
1102	40-41	0.38946540836187893
1102	42-43	0.4135217308502206
1102	44-45	0.46857801690163825
1102	46-47	0.4592518270660264
1102	48-49	0.5051884254209824
1102	50-51	0.5656695970078189
1102	52-53	0.541579509573296
1102	54-55	0.5256553353897857
1102	56-57	0.5450767113014052
1102	58-59	0.5683004300958316
1102	60-61	0.5379660225222835
1102	62-63	0.561912454026448
1102	64-65	0.5541429687519184
1102	66-67	0.5822104486814368
1102	68-69	0.5528578838214102
1102	70-71	0.5034209695386949
1102	72-73	0.4683261310283342
1102	74-75	0.45430757656586707
1102	76-77	0.4974242288374455
1102	78-79	0.47249824285581354
1102	80-81	0.4659228829696076
1102	82-83	0.4838498199529937
1102	84-85	0.4842609810263454
1102	86-87	0.4818986645238752
1102	88-89	0.4979694747930239
1102	90-91	0.5382464549654102
1102	92-93	0.5382983744405081
1102	94-95	0.5295880050684616
1102	96-97	0.4848291247178764
1102	98-99	0.5039347606762732
1102	100	0.5256348564707167
1103	1	0.11299068894814468
1103	2	0.12605139483550687
1103	3	0.12376517925581965
1103	4	0.09114422886234053
1103	5	0.1251364161408759
1103	6	0.12161166520692746
1103	7	0.0782925941437469
1103	8	0.09821457352596497
1103	9	0.16660820584485236
1103	10-11	0.17577612834932665
1103	12-13	0.16665933844002012
1103	14-15	0.2549582623428748
1103	16-17	0.26001472480120924
1103	18-19	0.22583350720869788
1103	20-21	0.2315497510190596
1103	22-23	0.280390793435366
1103	24-25	0.23338736061615606
1103	26-27	0.24563708789415983
1103	28-29	0.27204532696731576
1103	30-31	0.27142147092140334
1103	32-33	0.28090604270372665
1103	34-35	0.3159015090293025
1103	36-37	0.280856318392253
1103	38-39	0.3097927160280136
1103	40-41	0.2641289933265156
1103	42-43	0.30407134206840425
1103	44-45	0.24546525171403744
1103	46-47	0.27865768869957463
1103	48-49	0.2977155441526591
1103	50-51	0.31745671342576287
1103	52-53	0.2823049894893188
1103	54-55	0.291355401109783
1103	56-57	0.24416106181793396
1103	58-59	0.1866884931771935
1103	60-61	0.18407143543835502
1103	62-63	0.21015561311562436
1103	64-65	0.2486399645847328
1103	66-67	0.2493228791154536
1103	68-69	0.2253775690545936
1103	70-71	0.2357335506118261
1103	72-73	0.2419281041052841
1103	74-75	0.21871490274629934
1103	76-77	0.21733714367958612
1103	78-79	0.2431344469471881
1103	80-81	0.2660262627500032
1103	82-83	0.30542173412808893
1103	84-85	0.2869126723933917
1103	86-87	0.32630077248682454
1103	88-89	0.33493365369911743
1103	90-91	0.3500081202659828
1103	92-93	0.3596733747481835
1103	94-95	0.37953717667643616
1103	96-97	0.36744409164865743
1103	98-99	0.34963723859115703
1103	100	0.39533104298753585
1104	1	0.06564229464905935
1104	2	0.1116234787950603
1104	3	0.12138193329818137
1104	4	0.07153925377328818
1104	5	0.10200643345822158
1104	6	0.13387219072339462
1104	7	0.08430561963062644
1104	8	0.15866971606865832
1104	9	0.2043513489240283
1104	10-11	0.1579944058450522
1104	12-13	0.16769774824187778
1104	14-15	0.2158713876313385
1104	16-17	0.22249887686251668
1104	18-19	0.18989744333568837
1104	20-21	0.17569479072273708
1104	22-23	0.18903676606685593
1104	24-25	0.20583210485980885
1104	26-27	0.22975246325460574
1104	28-29	0.2286838677340981
1104	30-31	0.28282433400389806
1104	32-33	0.2561182492653842
1104	34-35	0.2459747626002553
1104	36-37	0.26208028812830975
1104	38-39	0.19941090595081334
1104	40-41	0.25638155049546896
1104	42-43	0.2469846803720941
1104	44-45	0.25524809452063124
1104	46-47	0.3245532402315021
1104	48-49	0.31543746460330624
1104	50-51	0.26628777262427406
1104	52-53	0.314862868488774
1104	54-55	0.3056435160433395
1104	56-57	0.2883082316739092
1104	58-59	0.23925323600074222
1104	60-61	0.2938470199482026
1104	62-63	0.329922321990864
1104	64-65	0.36362763731444403
1104	66-67	0.40455575604393346
1104	68-69	0.38306004224031653
1104	70-71	0.3734764570074134
1104	72-73	0.32425115353665745
1104	74-75	0.31363101813744265
1104	76-77	0.2700760856250426
1104	78-79	0.2878909981517168
1104	80-81	0.2579513287255537
1104	82-83	0.2879727887507073
1104	84-85	0.30489541815970966
1104	86-87	0.28433503638112967
1104	88-89	0.2611501481784728
1104	90-91	0.2845764685695542
1104	92-93	0.32443134605424717
1104	94-95	0.30890740109192905
1104	96-97	0.307101294916464
1104	98-99	0.2923592576599674
1104	100	0.23037457304437936
1105	1	-0.036431466505298715
1105	2	-0.0010567313182221483
1105	3	0.05716969989523335
1105	4	-0.06689011778546927
1105	5	-0.11716308112946194
1105	6	-0.050502057741894646
1105	7	-0.43214444682086395
1105	8	-0.3471769075627549
1105	9	-0.3156774467218213
1105	10-11	-0.3482684889084382
1105	12-13	-0.21708319916651675
1105	14-15	-0.18832779280027268
1105	16-17	-0.1619492593313936
1105	18-19	-0.14885326864456516
1105	20-21	-0.10440892106765887
1105	22-23	-0.1449047566215782
1105	24-25	-0.16846350934181942
1105	26-27	-0.1954583620096173
1105	28-29	-0.19220318055398877
1105	30-31	-0.1973111004622794
1105	32-33	-0.18328526949925106
1105	34-35	-0.24334719487077905
1105	36-37	-0.22022597413356948
1105	38-39	-0.24076598158794127
1105	40-41	-0.2087444700095631
1105	42-43	-0.22781050664139002
1105	44-45	-0.2399300587608053
1105	46-47	-0.1786638029936043
1105	48-49	-0.1598806323525821
1105	50-51	-0.11511467025908928
1105	52-53	-0.13804770679126932
1105	54-55	-0.1702550030377381
1105	56-57	-0.17023742999307245
1105	58-59	-0.11736280095893648
1105	60-61	-0.12125558431734618
1105	62-63	-0.12880078097730063
1105	64-65	-0.09757434357284467
1105	66-67	-0.10085410725585575
1105	68-69	-0.13338337906213127
1105	70-71	-0.11860347464248377
1105	72-73	-0.07720041598425809
1105	74-75	-0.12603711995508604
1105	76-77	-0.1048205190202296
1105	78-79	-0.10680688489366474
1105	80-81	-0.07261224320028248
1105	82-83	-0.04872259445288307
1105	84-85	-0.02677370088338904
1105	86-87	-0.04765154674651484
1105	88-89	0.0013749757210348434
1105	90-91	0.04115413770441734
1105	92-93	0.011152597873746117
1105	94-95	-0.06574997818786699
1105	96-97	-0.03602469394499508
1105	98-99	-0.06893994808650916
1105	100	-0.06070858624933351
1106	1	0.07069107225828475
1106	2	0.07300200398123735
1106	3	0.06765174875214797
1106	4	0.011989464341723988
1106	5	0.06017145117591127
1106	6	0.07079987780977604
1106	7	0.08094524679639648
1106	8	0.07724116108914103
1106	9	0.0707892161234227
1106	10-11	0.11297247103347985
1106	12-13	0.0930485325121424
1106	14-15	0.10885301074982578
1106	16-17	0.11341019344057202
1106	18-19	0.14684476236629251
1106	20-21	0.08063969462119758
1106	22-23	0.10821495906441214
1106	24-25	0.09879667236693024
1106	26-27	0.07168508711288979
1106	28-29	0.06688040594873712
1106	30-31	0.08150411454064255
1106	32-33	0.029094475038505152
1106	34-35	-0.009497670638175748
1106	36-37	0.0024700052498403124
1106	38-39	0.025957051094792405
1106	40-41	0.06473203539297145
1106	42-43	0.05738929416683902
1106	44-45	3.2865169938389727E-4
1106	46-47	0.016962456882389176
1106	48-49	-0.027645182427576742
1106	50-51	-0.03242548584668015
1106	52-53	-0.1173117003712747
1106	54-55	-0.11482272823907635
1106	56-57	-0.09890456478427723
1106	58-59	-0.08065323879868913
1106	60-61	-0.09792214350643746
1106	62-63	-0.10819810645761407
1106	64-65	-0.041511772518219914
1106	66-67	-0.050152816091944885
1106	68-69	-0.05098354816018258
1106	70-71	-0.048833333345299934
1106	72-73	-0.0984787661796247
1106	74-75	-0.0594644961648072
1106	76-77	-0.06400334314870548
1106	78-79	-0.06916626840230577
1106	80-81	-0.08747663038703735
1106	82-83	-0.10421045904069359
1106	84-85	-0.08065489517417745
1106	86-87	-0.0850532644769757
1106	88-89	-0.04155503010663608
1106	90-91	-0.026158290046765842
1106	92-93	-0.02833048276786343
1106	94-95	-0.016847622757140357
1106	96-97	-0.006553686617408516
1106	98-99	-0.025439057216850358
1106	100	0.011135872206999409
1107	1	-0.02225944631486243
1107	2	0.013451318087156494
1107	3	0.02034749424343829
1107	4	-0.09083757677365156
1107	5	-0.058813496027553924
1107	6	-0.061019819968962
1107	7	-0.04484950590082093
1107	8	-0.02230261977261705
1107	9	0.0164728534579055
1107	10-11	0.03303432933942929
1107	12-13	0.040042245367160945
1107	14-15	0.07424414517532085
1107	16-17	0.08656035789165628
1107	18-19	0.06448632639959584
1107	20-21	0.0680020288004144
1107	22-23	0.059277444823869985
1107	24-25	0.029387672257357167
1107	26-27	0.016959662157724154
1107	28-29	0.04128765153655678
1107	30-31	0.040238610829838706
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2216	30-31	-0.5532948325895006
2216	32-33	-0.5725859211868283
2216	34-35	-0.5437745608857654
2216	36-37	-0.6259914638719266
2216	38-39	-0.601301192170574
2216	40-41	-0.6576982439615122
2216	42-43	-0.6023484893278948
2216	44-45	-0.614770784371153
2216	46-47	-0.6742369476293462
2216	48-49	-0.6359323296582886
2216	50-51	-0.6307539563207527
2216	52-53	-0.6737081483825591
2216	54-55	-0.6713346645492635
2216	56-57	-0.6944842201885848
2216	58-59	-0.7680169202080336
2216	60-61	-0.6949309320461623
2216	62-63	-0.7189920318283853
2216	64-65	-0.6697183470744505
2216	66-67	-0.6367346149885691
2216	68-69	-0.7341505709695397
2216	70-71	-0.698987800097207
2216	72-73	-0.6992435051779182
2216	74-75	-0.6430776558951692
2216	76-77	-0.6350607680232514
2216	78-79	-0.6390259054169576
2216	80-81	-0.6724280441924009
2216	82-83	-0.7018758626174559
2216	84-85	-0.6540746686880752
2216	86-87	-0.5860774051260549
2216	88-89	-0.5756518212831452
2216	90-91	-0.5749757377940696
2216	92-93	-0.7533887891508613
2216	94-95	-0.693408401492988
2216	96-97	-0.7632296107818775
2216	98-99	-0.7610917329323428
2216	100	-0.7521077692081946
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56.0
3	316.0
4	181.0
5	271.0
6	313.0
7	524.0
8	1079.0
9	1729.0
10	2302.0
11	3296.0
12	4595.0
13	6562.0
14	7376.0
15	9927.0
16	13596.0
17	22037.0
18	24957.0
19	13607.0
20	9280.0
21	9028.0
22	9367.0
23	9591.0
24	10757.0
25	12731.0
26	15705.0
27	19987.0
28	25270.0
29	31759.0
30	40674.0
31	51555.0
32	65069.0
33	83028.0
34	115533.0
35	189771.0
36	347992.0
37	774040.0
38	1280294.0
39	703633.0
40	4960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.03043618924419	21.254878705732175	15.324279529665871	24.39040557535776
2	13.236056263633621	22.010162776238182	40.075946779132074	24.677834180996122
3	15.188808222422253	28.19261143431232	32.05236547849117	24.566214864774263
4	10.730602163185466	18.872183307113755	39.2617881677166	31.13542636198418
5	12.162468759145375	38.76649736358287	35.28207776793207	13.78895610933968
6	28.24076387267293	39.49165228049317	18.547737453438256	13.719846393395649
7	24.94944870279712	34.43183197085308	23.255738069333027	17.362981257016767
8	25.304658876889363	35.10289215621294	20.748363137270097	18.844085829627595
9	26.18631122876106	15.818477251151489	21.425872882989168	36.569338637098284
10-11	23.4453245530939	27.164910924688506	29.258583523591113	20.131180998626473
12-13	24.779937431616816	25.320846508621	29.138119501940984	20.7610965578212
14-15	22.162932719613902	26.371946400839413	27.301345893236068	24.163774986310617
16-17	20.591687255974637	29.600129806961856	27.882048502733287	21.92613443433022
18-19	20.69160445687564	27.77116959845496	30.995134023393806	20.542091921275595
20-21	22.109437057899207	26.712192575204934	30.692616502513033	20.485753864382826
22-23	21.78532033801823	26.835378371330094	30.586142822873867	20.793158467777808
24-25	21.46680269393126	26.93596037262701	30.598907448181027	20.998329485260697
26-27	21.174112682921358	27.018622773358516	30.685449702808558	21.121814840911572
28-29	21.07408563072819	26.967890006485323	30.954769974996836	21.003254387789646
30-31	21.291849927995603	27.027575811110626	30.73789914387547	20.942675117018304
32-33	21.230851633773398	27.058792337301107	30.755905485715264	20.954450543210232
34-35	21.487790610892013	26.91841206240506	30.515843773968083	21.077953552734847
36-37	21.3646473552456	27.021874581348715	30.37094463535545	21.24253342805023
38-39	21.285617047746026	27.084749262612075	30.39404685121972	21.235586838422183
40-41	21.29594245852939	26.911737300568983	30.454377546304144	21.33794269459748
42-43	21.32158401641512	27.13360318829426	30.32865295536304	21.21615983992758
44-45	21.52900991350917	27.14971570804807	30.003775433830743	21.317498944612016
46-47	21.58294839622746	27.2000113189928	29.66474749404765	21.552292790732093
48-49	21.636676202279396	27.327146465660384	29.233440492665252	21.802736839394967
50-51	21.657101428068497	27.44752289934181	29.13395863218065	21.761417040409043
52-53	21.64414100066344	27.547006275575985	29.098852271271603	21.710000452488977
54-55	21.687445573590626	27.64765999579884	29.029478974835882	21.635415455774655
56-57	21.62334684350641	27.51320957406373	29.014118509005097	21.84932507342476
58-59	21.698076411756023	27.326121332615806	29.221897249383783	21.753905006244388
60-61	21.712795138092254	27.467726640052614	29.163684057572002	21.65579416428313
62-63	21.570286951902084	27.31461465278932	29.451433026031754	21.663665369276845
64-65	21.63039787414333	27.281015757321143	29.35067457812737	21.737911790408152
66-67	21.54969177015348	27.348076157321373	29.232481934738175	21.869750137786976
68-69	21.754250809960325	27.378545464464356	29.078907687338617	21.788296038236705
70-71	21.69042855525882	27.31647185028149	29.07349028773274	21.919609306726954
72-73	21.70803309722322	27.424513338360423	29.025075801492118	21.842377762924237
74-75	21.754313064426697	27.47410886703589	28.814100072017183	21.95747799652023
76-77	21.771530206108853	27.33599253377683	28.73320717979021	22.1592700803241
78-79	21.701362555360422	27.43223231531931	28.870539013532248	21.995866115788015
80-81	21.545901835553373	27.51428316138816	28.920669327494856	22.01914567556361
82-83	21.609419942748602	27.44122095526575	28.9774846349414	21.97187446704425
84-85	21.686602939423853	27.403177524707107	28.72849938467245	22.18172015119659
86-87	21.738581793964038	27.461128867276997	28.71442455683053	22.085864781928436
88-89	21.62042552800962	27.352272853288166	28.769913524671082	22.257388094031132
90-91	21.587030270816644	27.377636037675845	28.682447999418763	22.352885692088748
92-93	21.525546506474434	27.442573147211156	28.77939632447238	22.25248402184203
94-95	21.483593591666974	27.38523544339939	28.821884388451334	22.3092865764823
96-97	21.58875233482968	27.485167601703232	28.57673473352159	22.3493453299455
98-99	21.388320857316316	27.436436937745935	28.733554365348308	22.44168783958944
100	21.496967961884554	27.385574244080917	28.744261791068137	22.37319600296639
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1526.0
1	981.5
2	1060.0
3	1569.5
4	964.5
5	785.5
6	2200.5
7	6251.5
8	11647.0
9	12938.5
10	8383.5
11	4226.5
12	3537.0
13	3784.5
14	4078.5
15	4330.0
16	4656.5
17	5229.0
18	5834.0
19	6437.0
20	7721.0
21	9509.5
22	12392.0
23	16829.0
24	21824.5
25	26818.5
26	33933.5
27	42993.0
28	54204.5
29	66258.5
30	73764.5
31	80265.0
32	88590.5
33	96830.5
34	104322.0
35	109403.5
36	112125.0
37	115248.0
38	117650.5
39	118001.0
40	119530.5
41	120600.5
42	120229.0
43	120845.0
44	121475.0
45	121778.5
46	122802.0
47	122231.5
48	118726.5
49	116976.0
50	118294.5
51	116901.5
52	111689.0
53	108253.0
54	106906.5
55	103361.5
56	93929.0
57	82376.0
58	75309.0
59	71562.5
60	63886.0
61	52970.5
62	45815.0
63	38432.0
64	29916.5
65	24123.5
66	18964.5
67	14217.5
68	11018.5
69	8482.0
70	6303.5
71	4554.5
72	3397.0
73	2608.0
74	1931.5
75	1339.5
76	942.5
77	682.5
78	418.0
79	242.0
80	149.5
81	84.0
82	39.5
83	21.0
84	11.5
85	8.0
86	6.0
87	3.5
88	3.0
89	4.5
90	4.0
91	2.0
92	2.0
93	1.5
94	3.0
95	7.0
96	12.5
97	14.0
98	6.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008794855035296685
2	2.5492333435642567E-5
3	2.8041566779206823E-4
4	0.0027276796776137544
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.5492333435642567E-5
24-25	1.2746166717821281E-4
26-27	6.118160024554216E-4
28-29	0.001121662671168273
30-31	0.001809955673930622
32-33	0.004779812519182981
34-35	0.005442613188509688
36-37	0.004626858518569125
38-39	0.0030080953454058226
40-41	0.003849342348782027
42-43	5.990698357376003E-4
44-45	5.098466687128513E-4
46-47	0.003505195847400853
48-49	0.0021541021753117965
50-51	9.432163371187748E-4
52-53	1.2746166717821283E-5
54-55	5.098466687128513E-4
56-57	6.37308335891064E-4
58-59	6.882930027623492E-4
60-61	2.0393866748514054E-4
62-63	0.0
64-65	0.0
66-67	6.628006693267066E-4
68-69	6.37308335891064E-4
70-71	0.002039386674851405
72-73	5.0984666871285134E-5
74-75	0.001860940340801907
76-77	3.823850015346384E-4
78-79	0.0011471550046039155
80-81	8.794855035296684E-4
82-83	0.0012491243383464857
84-85	0.001032439504143524
86-87	2.6766950107424693E-4
88-89	0.0017462248403415158
90-91	0.0019246711743910135
92-93	0.003250272513044427
94-95	0.0010961703377326303
96-97	0.002166848342029618
98-99	0.002256071509054367
100	0.0035434343475543167
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3922748.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.3998040441544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.92271890122448	43.38426272572632
2	13.655373761070441	15.403208085481209
3	4.024282571192285	6.80906245300652
4	1.6947052788034174	3.8232418254842715
5	0.9433349614977696	2.6601953488237067
6	0.5967213369348634	2.0192979883255275
7	0.4086780167867625	1.613455204474893
8	0.2827792422482103	1.2758955080442824
9	0.22297579896961034	1.131821223162731
>10	1.1246472432868015	11.414029930321124
>50	0.07493222408924607	2.9234598255098483
>100	0.04573151379238226	4.681594117474246
>500	0.0023054587995053433	0.8885972123241384
>1k	6.780760869479117E-4	0.6343553340793023
>5k	0.0	0.0
>10k+	1.356152173895819E-4	1.337523217761915
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22759	0.5801800166617892	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17311	0.44129778410440845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12391	0.31587550360104705	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.5492333435642564E-5	0.0	0.0	0.0
6	0.0	2.5492333435642564E-5	0.0	0.0	0.0
7	0.0	2.5492333435642564E-5	0.0	0.0	0.0
8	0.0	2.5492333435642564E-5	0.0	0.0	0.0
9	0.0	2.5492333435642564E-5	0.0	0.0	0.0
10-11	0.0	2.5492333435642564E-5	0.0	2.5492333435642564E-5	0.0
12-13	0.0	2.5492333435642564E-5	0.0	2.5492333435642564E-5	0.0
14-15	0.0	2.5492333435642564E-5	0.0	6.373083358910641E-5	0.0
16-17	0.0	2.5492333435642564E-5	0.0	1.0196933374257025E-4	2.5492333435642564E-5
18-19	2.5492333435642564E-5	5.098466687128513E-5	0.0	1.2746166717821281E-4	5.098466687128513E-5
20-21	5.098466687128513E-5	5.098466687128513E-5	0.0	2.4217716763860438E-4	6.373083358910641E-5
22-23	5.098466687128513E-5	5.098466687128513E-5	0.0	5.608313355841364E-4	7.64770003069277E-5
24-25	5.098466687128513E-5	5.098466687128513E-5	0.0	8.157546699405621E-4	7.64770003069277E-5
26-27	5.098466687128513E-5	5.098466687128513E-5	0.0	0.0010196933374257027	7.64770003069277E-5
28-29	5.098466687128513E-5	5.098466687128513E-5	0.0	0.0017589710070593369	7.64770003069277E-5
30-31	5.098466687128513E-5	5.098466687128513E-5	0.0	0.003925819349088955	7.64770003069277E-5
32-33	5.098466687128513E-5	5.098466687128513E-5	0.0	0.007481999863361093	7.64770003069277E-5
34-35	5.098466687128513E-5	5.098466687128513E-5	0.0	0.013638398388068771	7.64770003069277E-5
36-37	5.098466687128513E-5	5.098466687128513E-5	0.0	0.024612847932112897	7.64770003069277E-5
38-39	5.098466687128513E-5	5.098466687128513E-5	0.0	0.03944938599165687	7.64770003069277E-5
40-41	5.098466687128513E-5	5.098466687128513E-5	2.5492333435642564E-5	0.055216394221601794	7.64770003069277E-5
42-43	6.373083358910641E-5	5.098466687128513E-5	2.5492333435642564E-5	0.07058827128329426	7.64770003069277E-5
44-45	7.64770003069277E-5	5.098466687128513E-5	2.5492333435642564E-5	0.09045954519637764	7.64770003069277E-5
46-47	1.529540006138554E-4	5.098466687128513E-5	2.5492333435642564E-5	0.11536555496300044	7.64770003069277E-5
48-49	1.529540006138554E-4	7.64770003069277E-5	2.5492333435642564E-5	0.14291002124021224	7.64770003069277E-5
50-51	1.529540006138554E-4	7.64770003069277E-5	2.5492333435642564E-5	0.17084961868567647	7.64770003069277E-5
52-53	1.657001673316767E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.19815190779524966	7.64770003069277E-5
54-55	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.2289466465855059	7.64770003069277E-5
56-57	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.26826857090998457	7.64770003069277E-5
58-59	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.31344098575794316	7.64770003069277E-5
60-61	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.3637755981266194	7.64770003069277E-5
62-63	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.4144161184965234	7.64770003069277E-5
64-65	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.46838338837977866	7.64770003069277E-5
66-67	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.5322544298027811	7.64770003069277E-5
68-69	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.6093177537787287	7.64770003069277E-5
70-71	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.6919893911105175	7.64770003069277E-5
72-73	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.7820665513053604	7.64770003069277E-5
74-75	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.8746037216767429	7.64770003069277E-5
76-77	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	0.9753621695811202	7.64770003069277E-5
78-79	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	1.0935828658889126	7.64770003069277E-5
80-81	1.7844633404949796E-4	1.0196933374257025E-4	2.5492333435642564E-5	1.2307953506062588	7.64770003069277E-5
82-83	1.7844633404949796E-4	1.0196933374257025E-4	3.823850015346385E-5	1.3791734773684161	7.64770003069277E-5
84-85	1.7844633404949796E-4	1.0196933374257025E-4	5.098466687128513E-5	1.530954830644232	7.64770003069277E-5
86-87	1.7844633404949796E-4	1.0196933374257025E-4	5.098466687128513E-5	1.6929076249608692	7.64770003069277E-5
88	1.7844633404949796E-4	1.0196933374257025E-4	5.098466687128513E-5	1.8255569819932354	7.64770003069277E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7815	0.0	64.172874	1
GTATCAA	16170	0.0	46.65316	1
TCAACGC	21370	0.0	35.078785	4
ATCAACG	21475	0.0	34.907272	3
TATCAAC	21660	0.0	34.67422	2
CAACGCA	22475	0.0	33.311863	5
AACGCAG	23345	0.0	32.11069	6
GTGGTAT	2385	0.0	31.334646	1
ACGCAGA	26125	0.0	28.675755	7
CGCAGAG	26240	0.0	28.460527	8
GCAGAGT	28665	0.0	25.87247	9
GTACATG	18915	0.0	25.768461	1
TGGTATC	2860	0.0	25.307138	2
TACATGG	19460	0.0	24.513853	2
ACATGGG	19950	0.0	23.275682	3
TATAACG	290	5.456968E-12	22.689157	2
AGAGTAC	26920	0.0	22.643719	10-11
GAGTACT	17440	0.0	22.529037	12-13
AGTACTT	17985	0.0	22.251385	12-13
CAGAGTA	28085	0.0	21.687695	10-11
>>END_MODULE