FastQCFastQC Report
Wed 25 May 2016
SRR2431379_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431379_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349845
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245141.0432177441490822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33050.044.084581
GTATCAA55900.037.751221
TCAACGC69000.029.5622254
ATCAACG69450.029.438353
TACCTGG16850.027.8930852
CAACGCA74200.027.7438475
AACGCAG78600.026.3103496
TATCAAC81950.025.234822
GTACATG124750.024.63961
ACGCAGA84250.024.3785577
TACATGG124250.023.7552592
CGCAGAG87400.023.3385988
GTACCTG21850.022.585741
ACATGGG129950.021.772923
TATAACG2206.314258E-821.363572
CATGGGT27700.020.7002944
GCAGAGT93900.020.5217679
CATGGGG70200.020.1523574
ACCTGGG23750.019.9873053
TAATACG4550.018.5933484
Produced by FastQC (version 0.11.5)