##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431379_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2349845 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.643585002415055 33.0 31.0 34.0 26.0 34.0 2 30.747317376252475 33.0 31.0 34.0 26.0 34.0 3 30.8383089097366 34.0 31.0 34.0 26.0 34.0 4 34.02885935029757 37.0 35.0 37.0 30.0 37.0 5 34.20686938925759 37.0 35.0 37.0 30.0 37.0 6 34.28802921043729 37.0 35.0 37.0 30.0 37.0 7 34.26303564703204 37.0 35.0 37.0 30.0 37.0 8 34.245610667937676 37.0 35.0 37.0 30.0 37.0 9 35.71191674344478 39.0 37.0 39.0 30.0 39.0 10-11 35.73559873098013 39.0 37.0 39.0 30.0 39.0 12-13 35.671207888179865 39.0 37.0 39.0 30.0 39.0 14-15 36.98308718234607 40.0 38.0 41.0 30.0 41.0 16-17 36.96723932855146 40.0 38.0 41.0 30.0 41.0 18-19 36.956893327006675 40.0 38.0 41.0 30.0 41.0 20-21 36.980048896842135 40.0 38.0 41.0 30.0 41.0 22-23 36.97976079273314 40.0 38.0 41.0 30.0 41.0 24-25 36.99844904663925 40.0 38.0 41.0 30.0 41.0 26-27 36.992963578448794 40.0 38.0 41.0 30.0 41.0 28-29 36.9682174781741 40.0 38.0 41.0 30.0 41.0 30-31 36.898379467581904 40.0 38.0 41.0 30.0 41.0 32-33 36.81386708485027 40.0 37.0 41.0 30.0 41.0 34-35 36.74737503963027 40.0 37.0 41.0 30.0 41.0 36-37 36.651358493858105 40.0 37.0 41.0 29.5 41.0 38-39 36.54218405894857 40.0 37.0 41.0 29.0 41.0 40-41 36.44580748943015 40.0 37.0 41.0 28.5 41.0 42-43 36.31376643991412 40.0 36.5 41.0 28.0 41.0 44-45 36.170631041621895 40.0 36.0 41.0 28.0 41.0 46-47 36.03018390574697 40.0 36.0 41.0 28.0 41.0 48-49 35.87727701188802 40.0 35.0 41.0 27.0 41.0 50-51 35.722069753536935 40.0 35.0 41.0 27.0 41.0 52-53 35.52548146792661 39.0 35.0 41.0 27.0 41.0 54-55 35.33947345463211 39.0 35.0 41.0 27.0 41.0 56-57 35.12058263417374 39.0 35.0 41.0 26.5 41.0 58-59 34.87013185976096 38.5 34.0 40.0 26.0 41.0 60-61 34.664746823726674 38.0 34.0 40.0 26.0 41.0 62-63 34.47677102106735 38.0 34.0 40.0 26.0 41.0 64-65 34.20282805887197 37.0 34.0 40.0 26.0 41.0 66-67 33.87792599086323 37.0 33.5 40.0 25.0 41.0 68-69 33.5485261793863 36.0 33.0 39.5 25.0 41.0 70-71 33.284782613321305 36.0 33.0 39.0 24.5 41.0 72-73 33.03809549140475 35.5 33.0 39.0 24.0 41.0 74-75 32.693579576525266 35.0 33.0 38.5 23.5 40.0 76-77 32.35529002976792 35.0 33.0 37.0 23.0 39.5 78-79 32.026129170221864 35.0 32.5 37.0 22.0 39.0 80-81 31.705371630894803 35.0 32.0 36.0 20.5 39.0 82-83 31.399676999972336 35.0 32.0 36.0 20.0 37.5 84-85 31.130833735842153 35.0 32.0 35.5 19.5 37.0 86-87 30.887776215026946 35.0 32.0 35.0 18.0 37.0 88-89 30.656802257170156 35.0 31.5 35.0 17.5 36.0 90-91 30.4628560607189 35.0 31.0 35.0 13.5 36.0 92-93 30.267098893756824 35.0 31.0 35.0 7.0 36.0 94-95 30.05097591543272 35.0 31.0 35.0 2.0 36.0 96-97 29.857628694658583 34.0 31.0 35.0 2.0 35.0 98-99 29.73668667507857 34.5 31.0 35.0 2.0 35.0 100 29.69025786807215 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7037512022052326 1101 2 0.7518913435700654 1101 3 0.7846563193494944 1101 4 0.7611753059858799 1101 5 0.7584955009296124 1101 6 0.6906700992241284 1101 7 0.7353032401335966 1101 8 0.8128910007159789 1101 9 0.9036249733749884 1101 10-11 0.9470930180920902 1101 12-13 0.9353880171839606 1101 14-15 1.097574640231585 1101 16-17 1.0635684844435147 1101 18-19 1.1137365064563838 1101 20-21 1.0884912323811946 1101 22-23 1.0571244055115514 1101 24-25 1.1167978971173156 1101 26-27 1.1250321095405198 1101 28-29 1.1382521909009355 1101 30-31 1.0973551097854113 1101 32-33 1.0499995088253868 1101 34-35 1.118764714314679 1101 36-37 1.1540141354365048 1101 38-39 1.1432508106081727 1101 40-41 1.2253710600551955 1101 42-43 1.2370758099195882 1101 44-45 1.2354222201335645 1101 46-47 1.2119303040168035 1101 48-49 1.1623232396066783 1101 50-51 1.2033868223791515 1101 52-53 1.2936399574240554 1101 54-55 1.2598466903000585 1101 56-57 1.2289655429645592 1101 58-59 1.3140346189819354 1101 60-61 1.2988871170952336 1101 62-63 1.3171962174459324 1101 64-65 1.3568982097288753 1101 66-67 1.2417058270596968 1101 68-69 1.3159334051551639 1101 70-71 1.3189446461402525 1101 72-73 1.2552346282692781 1101 74-75 1.2639036810315574 1101 76-77 1.2773129279863653 1101 78-79 1.2995062563702362 1101 80-81 1.22308112285128 1101 82-83 1.2865363175223976 1101 84-85 1.2690511282187487 1101 86-87 1.291698530809704 1101 88-89 1.2368559665395154 1101 90-91 1.209111216037666 1101 92-93 1.232783271661397 1101 94-95 1.2982695955355474 1101 96-97 1.3831833405940515 1101 98-99 1.393289928605558 1101 100 1.4890325737448045 1102 1 0.33099621415065883 1102 2 0.42716257301248106 1102 3 0.457722744255193 1102 4 0.31114604951650193 1102 5 0.4569431272238944 1102 6 0.40632126959853565 1102 7 0.4180624605469845 1102 8 0.33287999195754026 1102 9 0.4997664497975123 1102 10-11 0.5631603010333635 1102 12-13 0.5350737125050031 1102 14-15 0.7239117996481212 1102 16-17 0.7167186776749688 1102 18-19 0.6387342052558296 1102 20-21 0.615881485929556 1102 22-23 0.5807938374735002 1102 24-25 0.6055775669933254 1102 26-27 0.6466313861078206 1102 28-29 0.6639803069462005 1102 30-31 0.6720273332539577 1102 32-33 0.6427349308933188 1102 34-35 0.6898596926093816 1102 36-37 0.6755179410902912 1102 38-39 0.6791129513491398 1102 40-41 0.6718008225574295 1102 42-43 0.6752527502710848 1102 44-45 0.6732140389608574 1102 46-47 0.6513414279509036 1102 48-49 0.5605654461689156 1102 50-51 0.5799379818835817 1102 52-53 0.5472860998487121 1102 54-55 0.6408755004059117 1102 56-57 0.6338106918256443 1102 58-59 0.6056451587117522 1102 60-61 0.6227436979506393 1102 62-63 0.60154712855946 1102 64-65 0.6815819276363726 1102 66-67 0.6992819112258104 1102 68-69 0.6750868408822868 1102 70-71 0.6962275180385404 1102 72-73 0.6910620312892846 1102 74-75 0.7457606458058379 1102 76-77 0.7193724446648702 1102 78-79 0.6336370616979181 1102 80-81 0.667902496494559 1102 82-83 0.6637719165168008 1102 84-85 0.5793999162145766 1102 86-87 0.5736030922748867 1102 88-89 0.6491972935574601 1102 90-91 0.6876287803267367 1102 92-93 0.6881215712707736 1102 94-95 0.56653504537978 1102 96-97 0.5644493940697863 1102 98-99 0.5386682159110272 1102 100 0.6415314462721682 1103 1 0.2080345112508759 1103 2 0.2939471815927561 1103 3 0.32936519880786364 1103 4 0.33228033318948036 1103 5 0.22442699526504128 1103 6 0.3139001716046863 1103 7 0.39678936831413836 1103 8 0.34477171220839153 1103 9 0.5272069968665392 1103 10-11 0.4975113369943074 1103 12-13 0.48966267359551807 1103 14-15 0.5101894848112565 1103 16-17 0.5339992093208323 1103 18-19 0.5685753238895117 1103 20-21 0.5678832096959852 1103 22-23 0.6044924505219527 1103 24-25 0.623513129882447 1103 26-27 0.64535466489302 1103 28-29 0.6361403450505136 1103 30-31 0.6411537692534566 1103 32-33 0.5840751592923752 1103 34-35 0.6422941724742088 1103 36-37 0.7236933570056436 1103 38-39 0.6590816513797577 1103 40-41 0.578924740950697 1103 42-43 0.5448783981010621 1103 44-45 0.568083338907563 1103 46-47 0.5176157109434527 1103 48-49 0.522801579488636 1103 50-51 0.4755326245989622 1103 52-53 0.3912303496133944 1103 54-55 0.4225588944112886 1103 56-57 0.37313434031337067 1103 58-59 0.3669339009292898 1103 60-61 0.4076013171116628 1103 62-63 0.39106957721503477 1103 64-65 0.42857769760428255 1103 66-67 0.4271291310854224 1103 68-69 0.42856986972134337 1103 70-71 0.5091398894648478 1103 72-73 0.4332219559879107 1103 74-75 0.40789357995115694 1103 76-77 0.46486445523428443 1103 78-79 0.4741099634092123 1103 80-81 0.4432310894702063 1103 82-83 0.4313846134174284 1103 84-85 0.30203886653154655 1103 86-87 0.3699965646435892 1103 88-89 0.45901663829940276 1103 90-91 0.4123786640859102 1103 92-93 0.4753212446623962 1103 94-95 0.3717272113149832 1103 96-97 0.44962749769990396 1103 98-99 0.29123742173100453 1103 100 0.22685720410566645 1104 1 0.32374346689791267 1104 2 0.3774806759621647 1104 3 0.4208575042320568 1104 4 0.43231435489533254 1104 5 0.42834856389249154 1104 6 0.39868679880617464 1104 7 0.4453961794596495 1104 8 0.5296989624607207 1104 9 0.616180562001098 1104 10-11 0.5086432391478866 1104 12-13 0.5896139091504651 1104 14-15 0.6017197811403108 1104 16-17 0.6945844960671081 1104 18-19 0.6355300409990363 1104 20-21 0.5985072811869259 1104 22-23 0.49555381433874146 1104 24-25 0.57289393483601 1104 26-27 0.5355382686989145 1104 28-29 0.534020792776154 1104 30-31 0.6593032152666751 1104 32-33 0.6251756480708792 1104 34-35 0.5929076972363347 1104 36-37 0.667600069488202 1104 38-39 0.658673390909577 1104 40-41 0.6535417132456871 1104 42-43 0.6738762320524572 1104 44-45 0.6033065780007689 1104 46-47 0.7068937703453457 1104 48-49 0.6744578695350256 1104 50-51 0.5607592658627496 1104 52-53 0.6151634856208332 1104 54-55 0.6299211915568677 1104 56-57 0.719941403219508 1104 58-59 0.6543900979830113 1104 60-61 0.6775210258858593 1104 62-63 0.6998698584611418 1104 64-65 0.7338607014940948 1104 66-67 0.7982929002368024 1104 68-69 0.7650694898123049 1104 70-71 0.7693333595654366 1104 72-73 0.7245033268358441 1104 74-75 0.7977386677838609 1104 76-77 0.8101705938840738 1104 78-79 0.7315317985400185 1104 80-81 0.7404299690220277 1104 82-83 0.7533439234572299 1104 84-85 0.757236816156734 1104 86-87 0.787963994530525 1104 88-89 0.797294459549363 1104 90-91 0.876651336717714 1104 92-93 0.8425345267363582 1104 94-95 0.7154881974908278 1104 96-97 0.7936685959205718 1104 98-99 0.7811205004685746 1104 100 0.8153544653583467 1105 1 0.19022124132567342 1105 2 0.26185007160298923 1105 3 0.20932884408785668 1105 4 0.31814840844320713 1105 5 0.288230433672382 1105 6 0.2884863403437876 1105 7 0.31586338156578364 1105 8 0.3851358565740881 1105 9 0.32020170109311863 1105 10-11 0.33940915652542003 1105 12-13 0.41264834795385497 1105 14-15 0.41551063777523467 1105 16-17 0.43297348686351 1105 18-19 0.40111243637652194 1105 20-21 0.43990753786202674 1105 22-23 0.3908103905538596 1105 24-25 0.4483307907846594 1105 26-27 0.48675109149005635 1105 28-29 0.5027657077607728 1105 30-31 0.5675016218548805 1105 32-33 0.546757554939937 1105 34-35 0.5156580517345475 1105 36-37 0.5436099166801327 1105 38-39 0.5073113267116582 1105 40-41 0.4906657864067654 1105 42-43 0.5762041239061233 1105 44-45 0.5340291090295537 1105 46-47 0.572778343575294 1105 48-49 0.6276675746154083 1105 50-51 0.5818856073960674 1105 52-53 0.6713089945179007 1105 54-55 0.6099209695075771 1105 56-57 0.5858136596531267 1105 58-59 0.5411265737069471 1105 60-61 0.5782257895022553 1105 62-63 0.6441115480063928 1105 64-65 0.6340978605400238 1105 66-67 0.6891942746177975 1105 68-69 0.6350548623155845 1105 70-71 0.7018591736623137 1105 72-73 0.6544891052729866 1105 74-75 0.626707373753483 1105 76-77 0.6203546241688187 1105 78-79 0.5145906003135039 1105 80-81 0.5147102777954409 1105 82-83 0.5781472970797878 1105 84-85 0.6369154206324872 1105 86-87 0.6122983757745786 1105 88-89 0.6547023880272889 1105 90-91 0.6638461533934752 1105 92-93 0.6575246229912537 1105 94-95 0.6386733695946347 1105 96-97 0.6826770868770922 1105 98-99 0.7102668285193303 1105 100 0.6913456638797761 1106 1 0.027045831355376748 1106 2 0.03923136123404092 1106 3 -0.001163651137954247 1106 4 0.056109826618140346 1106 5 -0.015635180976211416 1106 6 0.04670420602246139 1106 7 0.04445563576177136 1106 8 0.11528532145182879 1106 9 0.04849192398176427 1106 10-11 0.07321746324702616 1106 12-13 0.05768535020747123 1106 14-15 0.010952490675769866 1106 16-17 0.08973758858861913 1106 18-19 0.06909042565396817 1106 20-21 0.07078015658044023 1106 22-23 0.07286665830272199 1106 24-25 0.11937799727256504 1106 26-27 0.17140643717070958 1106 28-29 0.09269973221895356 1106 30-31 0.11455158434830537 1106 32-33 0.09206949013938726 1106 34-35 0.13825332350883457 1106 36-37 0.20481781991043135 1106 38-39 0.20563799283458906 1106 40-41 0.20237794920348762 1106 42-43 0.24398846727880397 1106 44-45 0.23020898005454882 1106 46-47 0.19329647647325032 1106 48-49 0.12824139388212785 1106 50-51 0.11415409336098037 1106 52-53 0.1575897753689688 1106 54-55 0.18806503498249327 1106 56-57 0.11700754155253179 1106 58-59 0.15722571912324668 1106 60-61 0.0910618787606694 1106 62-63 0.1399264638647395 1106 64-65 0.20020475179249786 1106 66-67 0.1998807937902285 1106 68-69 0.09821150693196046 1106 70-71 0.13910704186822898 1106 72-73 0.15547338146681966 1106 74-75 0.10268868593835379 1106 76-77 0.15006083095642708 1106 78-79 0.1804930996205627 1106 80-81 0.17582495751436156 1106 82-83 0.25586147266784565 1106 84-85 0.23047418273364428 1106 86-87 0.23812650861944817 1106 88-89 0.2320169331633153 1106 90-91 0.21193746031310567 1106 92-93 0.23923354267152064 1106 94-95 0.2541547785421301 1106 96-97 0.24794022089550438 1106 98-99 0.35425323638659023 1106 100 0.37337981215872773 1107 1 -0.12258388995575942 1107 2 -0.06206779734311496 1107 3 -0.08538489220997647 1107 4 -0.11211036738782809 1107 5 -0.12815010984756015 1107 6 -0.05104495764064865 1107 7 -0.1023212573684944 1107 8 -0.13177169029849978 1107 9 -0.12170859058860373 1107 10-11 -0.14861130304313974 1107 12-13 -0.03901834788469216 1107 14-15 -0.036292025564783614 1107 16-17 -0.11575634276737645 1107 18-19 -0.025214658947120938 1107 20-21 -0.12268994264186972 1107 22-23 -0.02357225015938269 1107 24-25 -0.08170609308525201 1107 26-27 0.00859125926551485 1107 28-29 0.008710168735952095 1107 30-31 -0.015534741694693821 1107 32-33 6.508081195448767E-4 1107 34-35 0.0575643654320217 1107 36-37 0.09794050944752541 1107 38-39 -0.022818801108932973 1107 40-41 -0.03274894709215914 1107 42-43 -0.09371316584547174 1107 44-45 0.04804641166982293 1107 46-47 0.0078091888651243835 1107 48-49 -0.024293651176570563 1107 50-51 -0.018041515932587515 1107 52-53 -0.06320351942493829 1107 54-55 -0.03703698638135222 1107 56-57 -5.19895916923474E-4 1107 58-59 0.029275919965016328 1107 60-61 -0.0633925976956391 1107 62-63 0.019417733453657604 1107 64-65 0.05898285701552197 1107 66-67 0.0238966975284427 1107 68-69 0.09320813882209222 1107 70-71 0.0415058331898166 1107 72-73 0.07273930138761386 1107 74-75 0.008507300240331972 1107 76-77 0.1635720019134297 1107 78-79 0.12071629825084074 1107 80-81 0.06530681631266688 1107 82-83 0.1841151486023236 1107 84-85 0.17405738216060485 1107 86-87 0.16266832830926603 1107 88-89 0.10721448507737463 1107 90-91 0.07866565633639055 1107 92-93 0.13832639367160482 1107 94-95 0.2619102871978569 1107 96-97 0.20244384970489548 1107 98-99 0.08439027969116708 1107 100 0.1410559870671264 1108 1 -0.18971307946202032 1108 2 -0.2276595994315116 1108 3 -0.15864549688407337 1108 4 -0.1448295455321258 1108 5 -0.14273903901209906 1108 6 -0.16202742227313394 1108 7 -0.0753715715487715 1108 8 -0.16302490306993178 1108 9 -0.1386102966966476 1108 10-11 -0.2178447227013578 1108 12-13 -0.1976643909708784 1108 14-15 -0.2033569770793875 1108 16-17 -0.1360610233525037 1108 18-19 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-0.8168851904662375 2215 68-69 -0.9872537255597251 2215 70-71 -1.0598959512564505 2215 72-73 -1.05772626068417 2215 74-75 -1.001282350285429 2215 76-77 -0.8788209663237403 2215 78-79 -0.8771466332908489 2215 80-81 -0.8354777156939477 2215 82-83 -0.8912755467008253 2215 84-85 -0.9225500363871362 2215 86-87 -0.9594907823079453 2215 88-89 -1.0608747778126748 2215 90-91 -0.9518577598579192 2215 92-93 -0.9013853566708434 2215 94-95 -0.9055932027015601 2215 96-97 -0.9120130054523337 2215 98-99 -0.930054108033552 2215 100 -0.9866540433586444 2216 1 -0.5440114187836684 2216 2 -0.6276033741133986 2216 3 -0.8741309887490445 2216 4 -0.8422259953965465 2216 5 -0.8015031782932169 2216 6 -0.843479703422247 2216 7 -0.7332688088028618 2216 8 -0.8792984617669006 2216 9 -0.9453246460635825 2216 10-11 -0.9947150324616558 2216 12-13 -0.9989006921974521 2216 14-15 -1.0142511599329964 2216 16-17 -1.0173210706522013 2216 18-19 -1.154165881773885 2216 20-21 -0.9948910811617964 2216 22-23 -1.0397854095054342 2216 24-25 -1.1315361851289154 2216 26-27 -1.1922255284746512 2216 28-29 -1.0028570901518563 2216 30-31 -1.0959885318357152 2216 32-33 -1.1574168717632247 2216 34-35 -1.1585162757745735 2216 36-37 -1.13159367095691 2216 38-39 -1.144200455507665 2216 40-41 -1.2655845764238975 2216 42-43 -1.3330010911636236 2216 44-45 -1.2545438757500165 2216 46-47 -1.1168213889585417 2216 48-49 -1.2924801659638874 2216 50-51 -1.1680487781854723 2216 52-53 -1.2229705664850528 2216 54-55 -1.3853940634468032 2216 56-57 -1.307291874837702 2216 58-59 -1.276563398734993 2216 60-61 -1.2002244434655225 2216 62-63 -1.2738434351363068 2216 64-65 -1.3513244369468111 2216 66-67 -1.3762162269088947 2216 68-69 -1.3998611552941185 2216 70-71 -1.6238549461865333 2216 72-73 -1.5448201789802205 2216 74-75 -1.5361945267555441 2216 76-77 -1.5024326373000036 2216 78-79 -1.392414214500164 2216 80-81 -1.398944887620381 2216 82-83 -1.4063158265600535 2216 84-85 -1.2813893434621306 2216 86-87 -1.269529121561412 2216 88-89 -1.4222207201675552 2216 90-91 -1.3060468698994008 2216 92-93 -1.4673774228939536 2216 94-95 -1.4933776395583713 2216 96-97 -1.5959737701024999 2216 98-99 -1.7634775660010575 2216 100 -1.7410974219134765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51736.0 3 10415.0 4 7928.0 5 7627.0 6 5161.0 7 4628.0 8 5023.0 9 5310.0 10 5457.0 11 5771.0 12 6529.0 13 7038.0 14 7232.0 15 7190.0 16 6973.0 17 6925.0 18 6831.0 19 6737.0 20 6618.0 21 7115.0 22 8961.0 23 15313.0 24 24571.0 25 27430.0 26 25713.0 27 19964.0 28 22509.0 29 25196.0 30 29511.0 31 36342.0 32 47608.0 33 64637.0 34 93093.0 35 152208.0 36 264277.0 37 481683.0 38 609857.0 39 220740.0 40 1988.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.52983181833657 21.56610662853641 13.464023082422633 22.440038470704387 2 15.936285159233906 22.85508193093587 38.425257836155154 22.78337507367507 3 17.33931386963821 29.50479712491675 29.5666309905547 23.589258014890344 4 12.30749262185378 19.998978656039014 37.845687694294725 29.84784102781247 5 12.766501620319637 40.12822122310195 33.334581642618986 13.77069551395943 6 28.046445616625775 40.141456138596375 18.438577863646323 13.37352038113152 7 25.037396083571466 35.238494453889516 22.924235428294207 16.79987403424481 8 24.29300656000715 36.7408063085012 20.8023507933502 18.16383633814145 9 25.326819428515496 18.801793309771494 20.644978711361812 35.226408550351195 10-11 22.987239583887447 29.240800989001404 28.053105630371366 19.718853796739786 12-13 23.70158457259947 27.82111160523354 27.481536016205325 20.99576780596167 14-15 21.86188876287585 28.6527409254653 25.271326406635332 24.214043905023523 16-17 21.370622317642226 31.36408997189176 25.620221759307526 21.645065951158482 18-19 21.21454819360426 30.272315833597535 27.446597541539973 21.066538431258234 20-21 23.334347584627924 29.193521274807487 26.49402407392828 20.97810706663631 22-23 22.59440516289372 29.510797520687536 26.572178164942795 21.322619151475948 24-25 22.179377788747768 29.285527343292856 26.733742012771057 21.801352855188323 26-27 22.00164266153725 29.465475382418845 26.719868757301015 21.813013198742894 28-29 21.92159482859508 29.26095125423166 27.05889111835036 21.7585627988229 30-31 22.386795724824402 29.584227895882492 26.35352544529533 21.675450933997777 32-33 21.593551915126316 29.53333092182676 27.023612195698014 21.84950496734891 34-35 22.164078907332186 28.730192842506636 27.003334262472634 22.102393987688547 36-37 22.522783417629675 29.268079383959368 26.457085467339336 21.752051731071624 38-39 22.097202155886876 29.152241956384355 26.804342414074117 21.946213473654648 40-41 22.111883975326034 28.974102547189283 26.77263819528522 22.141375282199462 42-43 21.668429194266004 29.365213450248845 27.04150699301443 21.924850362470714 44-45 22.03053818443344 29.034532064880874 26.86743168166411 22.067498069021575 46-47 22.147248009975126 29.345169575014523 26.605010117688614 21.902572297321736 48-49 21.77758533009624 28.964378501560738 27.111979726322378 22.146056442020644 50-51 22.095712695943774 28.92097138321889 26.701825013990284 22.281490906847047 52-53 21.858250225014842 29.204266664397018 26.779808881011302 22.157674229576845 54-55 22.039496222091245 29.154242088307953 26.72548614908643 22.080775540514374 56-57 21.963406096997886 29.236843281152584 26.63433120056855 22.165419421280976 58-59 21.957022697241733 28.580948956207752 27.00393004644987 22.458098300100644 60-61 22.425351459351575 29.07351335939179 26.506556815449528 21.994578365807108 62-63 21.81657126673701 29.07077468317584 26.915355462882758 22.197298587204386 64-65 21.9971789622675 28.769563262590353 26.85677711865615 22.37648065648599 66-67 21.9892801440095 28.947441214207746 26.646119212118247 22.41715942966451 68-69 22.19220842225764 28.980337000951128 26.765531343556702 22.06192323323453 70-71 22.393498294568364 28.689807200049366 26.672035815128233 22.244658690254038 72-73 21.86293138483602 28.891607744340586 27.03580448923227 22.209656381591124 74-75 21.972023686668695 28.692615895942076 26.88422002302279 22.451140394366437 76-77 22.153567575733717 28.914970987448108 26.66301394347287 22.268447493345306 78-79 22.066668227053274 28.84832829399386 26.86375058780473 22.22125289114814 80-81 22.078669018594844 28.943355838363804 26.693824486295902 22.284150656745442 82-83 21.778970292689827 29.05890501171887 27.001682027625257 22.160442667966045 84-85 21.93582349716916 28.66209158782415 26.778190951523346 22.623893963483347 86-87 22.173549463363152 28.95913303997753 26.664638142496024 22.202679354163298 88-89 21.72956890896366 28.78359822799716 26.994402219280563 22.492430643758617 90-91 21.876401721004857 28.827278182305655 26.636720728235293 22.659599368454202 92-93 21.865050360615413 28.910493541478488 26.895771027565086 22.328685070341017 94-95 21.91764382200995 28.483485122492052 26.969998155166458 22.628872900331537 96-97 21.90001866109515 28.982617094116392 26.76556408745649 22.35180015733197 98-99 21.491306651459603 28.832488929838263 26.94127335054058 22.734931068161554 100 21.720860438730806 28.759142353022288 26.889987054096174 22.63001015415073 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 21114.0 1 13651.0 2 4816.0 3 2689.0 4 1559.5 5 1090.0 6 966.5 7 1048.0 8 1311.5 9 1395.5 10 1055.0 11 736.5 12 694.5 13 696.5 14 732.5 15 793.5 16 892.0 17 1084.0 18 1308.0 19 1641.5 20 2153.5 21 2885.0 22 3908.0 23 5426.5 24 7404.0 25 9779.0 26 13006.0 27 17011.0 28 22318.0 29 28129.5 30 32843.5 31 37661.5 32 42596.5 33 46999.5 34 51345.5 35 56351.5 36 61046.5 37 65392.5 38 70457.0 39 74139.0 40 78718.5 41 82830.0 42 84013.0 43 86832.5 44 87646.5 45 85933.0 46 85237.5 47 84190.0 48 82606.5 49 81994.5 50 82801.0 51 80571.5 52 74933.0 53 69677.5 54 65899.5 55 62487.0 56 56197.0 57 48740.5 58 44512.5 59 41442.5 60 37090.0 61 31996.0 62 26620.5 63 20876.5 64 15899.0 65 12418.5 66 9733.5 67 7380.0 68 5381.0 69 3945.5 70 2941.5 71 2240.0 72 1627.5 73 1163.0 74 898.5 75 686.0 76 484.0 77 372.5 78 273.5 79 180.0 80 130.5 81 98.5 82 86.0 83 70.0 84 47.0 85 39.5 86 32.5 87 34.5 88 35.5 89 30.5 90 27.5 91 22.0 92 15.5 93 13.0 94 13.5 95 12.0 96 9.0 97 9.5 98 11.0 99 12.0 100 29.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.127799918718043E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.1277999187180433E-5 64-65 2.1277999187180433E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0638999593590215E-4 84-85 5.532279788666911E-4 86-87 0.0010638999593590216 88-89 0.003170421878889884 90-91 0.002340579910589847 92-93 0.0018086299309103366 94-95 0.001680961935787254 96-97 0.0015532939406641716 98-99 0.0025533599024616517 100 0.0028512518910821776 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2349845.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.542575396886186 #Duplication Level Percentage of deduplicated Percentage of total 1 77.65971381123605 46.24059364906125 2 13.515736991392584 16.095235777089535 3 4.034480706839275 7.206701150227807 4 1.5999064926655187 3.810502118700175 5 0.8455951642375396 2.517445691092803 6 0.5082744149254798 1.8158380603805158 7 0.3381638790323079 1.4094603784649284 8 0.23419453001413104 1.1155636368723778 9 0.16730929215646032 0.8965823528543128 >10 0.9848352964180049 10.74426006627226 >50 0.07717936872077538 3.148718650234898 >100 0.03346290195204503 3.498069010815156 >500 0.001003756608657084 0.3911274393106527 >1k 7.169690061827145E-5 0.06339491894517929 >5k 0.0 0.0 >10k+ 7.169690061827145E-5 1.0465070996781987 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24514 1.0432177441490822 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.127799918718043E-5 0.0 20-21 0.0 0.0 0.0 1.0638999593590215E-4 0.0 22-23 0.0 0.0 0.0 4.681159821179695E-4 0.0 24-25 0.0 0.0 0.0 0.001234123952856465 0.0 26-27 0.0 0.0 0.0 0.0019575759252205994 0.0 28-29 0.0 0.0 0.0 0.0035747038634463125 0.0 30-31 0.0 0.0 0.0 0.008319697682187549 0.0 32-33 0.0 0.0 0.0 0.016022333387946865 0.0 34-35 2.127799918718043E-5 0.0 0.0 0.028469962912447418 0.0 36-37 4.255599837436086E-5 0.0 0.0 0.048109556162214955 0.0 38-39 8.511199674872172E-5 0.0 0.0 0.07294098121365453 0.0 40-41 8.511199674872172E-5 0.0 0.0 0.10126199813179168 0.0 42-43 1.0638999593590215E-4 0.0 0.0 0.12664665116209792 0.0 44-45 1.2766799512308258E-4 0.0 0.0 0.16049994786890198 0.0 46-47 1.2766799512308258E-4 0.0 0.0 0.20094942432373197 0.0 48-49 1.2766799512308258E-4 0.0 0.0 0.24310114071353642 0.0 50-51 1.2766799512308258E-4 0.0 0.0 0.2884020009830436 0.0 52-53 1.2766799512308258E-4 0.0 0.0 0.33476676121190974 0.0 54-55 1.2766799512308258E-4 0.0 0.0 0.3828763173741247 0.0 56-57 1.2766799512308258E-4 0.0 0.0 0.44432717902670177 0.0 58-59 1.2766799512308258E-4 0.0 0.0 0.5107145364907046 0.0 60-61 1.2766799512308258E-4 0.0 0.0 0.5857833176230772 0.0 62-63 1.2766799512308258E-4 0.0 0.0 0.6633416246603498 0.0 64-65 1.2766799512308258E-4 0.0 0.0 0.7467726594732844 0.0 66-67 1.2766799512308258E-4 0.0 0.0 0.8486942755798788 0.0 68-69 1.2766799512308258E-4 0.0 0.0 0.9625954052288554 0.0 70-71 1.2766799512308258E-4 0.0 0.0 1.089093110396643 0.0 72-73 1.2766799512308258E-4 0.0 0.0 1.2258255331734647 0.0 74-75 1.2766799512308258E-4 0.0 0.0 1.3697711976747402 0.0 76-77 1.2766799512308258E-4 0.0 0.0 1.5240792477801728 0.0 78-79 1.2766799512308258E-4 0.0 0.0 1.7006015290370216 0.0 80-81 1.2766799512308258E-4 0.0 0.0 1.8995295434379713 0.0 82-83 1.2766799512308258E-4 0.0 0.0 2.118097151088689 0.0 84-85 1.2766799512308258E-4 0.0 0.0 2.342558764514255 0.0 86-87 1.2766799512308258E-4 0.0 0.0 2.5817447533773503 0.0 88 1.2766799512308258E-4 0.0 0.0 2.7732467460619743 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3305 0.0 44.08458 1 GTATCAA 5590 0.0 37.75122 1 TCAACGC 6900 0.0 29.562225 4 ATCAACG 6945 0.0 29.43835 3 TACCTGG 1685 0.0 27.893085 2 CAACGCA 7420 0.0 27.743847 5 AACGCAG 7860 0.0 26.310349 6 TATCAAC 8195 0.0 25.23482 2 GTACATG 12475 0.0 24.6396 1 ACGCAGA 8425 0.0 24.378557 7 TACATGG 12425 0.0 23.755259 2 CGCAGAG 8740 0.0 23.338598 8 GTACCTG 2185 0.0 22.58574 1 ACATGGG 12995 0.0 21.77292 3 TATAACG 220 6.314258E-8 21.36357 2 CATGGGT 2770 0.0 20.700294 4 GCAGAGT 9390 0.0 20.521767 9 CATGGGG 7020 0.0 20.152357 4 ACCTGGG 2375 0.0 19.987305 3 TAATACG 455 0.0 18.593348 4 >>END_MODULE