##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431379_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2349845
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78454110803053	34.0	31.0	34.0	31.0	34.0
2	32.89705363545255	34.0	31.0	34.0	31.0	34.0
3	32.969607357080996	34.0	31.0	34.0	31.0	34.0
4	36.36214686500599	37.0	37.0	37.0	35.0	37.0
5	36.32413499613804	37.0	37.0	37.0	35.0	37.0
6	36.35733973942962	37.0	37.0	37.0	35.0	37.0
7	36.22973600386408	37.0	37.0	37.0	35.0	37.0
8	36.28088022827038	37.0	37.0	37.0	35.0	37.0
9	38.10241781904764	39.0	39.0	39.0	37.0	39.0
10-11	38.09288719043171	39.0	39.0	39.0	36.0	39.0
12-13	38.07823664965136	39.0	39.0	39.0	35.0	39.0
14-15	39.559640529481726	41.0	39.5	41.0	37.0	41.0
16-17	39.56479129474497	41.0	39.5	41.0	37.0	41.0
18-19	39.63865680502331	41.0	40.0	41.0	37.0	41.0
20-21	39.63373860829118	41.0	40.0	41.0	37.0	41.0
22-23	39.55238515731889	41.0	40.0	41.0	36.5	41.0
24-25	39.520210481967965	41.0	40.0	41.0	36.0	41.0
26-27	39.4295757805302	41.0	39.0	41.0	36.0	41.0
28-29	39.34079907398147	41.0	39.0	41.0	36.0	41.0
30-31	39.16071336620075	41.0	39.0	41.0	35.0	41.0
32-33	38.93924195000096	41.0	39.0	41.0	35.0	41.0
34-35	38.82477886839345	41.0	38.0	41.0	35.0	41.0
36-37	38.71058835795552	41.0	38.0	41.0	35.0	41.0
38-39	38.59139390044875	41.0	38.0	41.0	35.0	41.0
40-41	38.48891182184357	41.0	38.0	41.0	35.0	41.0
42-43	38.34605154808083	41.0	38.0	41.0	35.0	41.0
44-45	38.12667303588109	40.5	38.0	41.0	34.0	41.0
46-47	37.79706470001213	40.0	38.0	41.0	33.5	41.0
48-49	37.504291772436055	40.0	38.0	41.0	33.0	41.0
50-51	37.3394581344727	40.0	37.0	41.0	33.0	41.0
52-53	37.20869759494775	40.0	37.0	41.0	33.0	41.0
54-55	37.04678053233298	40.0	37.0	41.0	33.0	41.0
56-57	36.86512152929236	40.0	36.0	41.0	32.0	41.0
58-59	36.6618168432386	40.0	35.5	41.0	32.0	41.0
60-61	36.44156167747234	39.0	35.0	41.0	31.5	41.0
62-63	36.18319484902196	39.0	35.0	41.0	31.0	41.0
64-65	35.88862584553449	38.5	35.0	41.0	31.0	41.0
66-67	35.584056820769035	37.5	35.0	40.5	31.0	41.0
68-69	35.29121750583549	37.0	35.0	40.0	30.0	41.0
70-71	35.0192199911058	37.0	35.0	39.5	30.0	41.0
72-73	34.74989861033387	36.0	35.0	39.0	30.0	41.0
74-75	34.44613793675753	36.0	35.0	39.0	30.0	41.0
76-77	34.132864933644555	35.0	35.0	37.5	30.0	40.0
78-79	33.82902468035126	35.0	35.0	37.0	30.0	39.0
80-81	33.540345639818796	35.0	35.0	37.0	30.0	39.0
82-83	33.25046545623222	35.0	34.0	36.0	29.0	38.5
84-85	33.01474671733668	35.0	34.0	36.0	29.0	37.0
86-87	32.820901591381556	35.0	34.0	36.0	29.0	37.0
88-89	32.6247173749758	35.0	34.0	35.0	29.0	36.5
90-91	32.450901442435566	35.0	34.0	35.0	29.0	36.0
92-93	32.321214165189616	35.0	34.0	35.0	29.0	36.0
94-95	32.20763114162849	35.0	34.0	35.0	29.0	36.0
96-97	32.084004476891025	35.0	34.0	35.0	29.0	36.0
98-99	31.97422468290462	35.0	34.0	35.0	28.5	35.5
100	31.874882385859493	35.0	34.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2761279262937606
1101	2	0.2824911315097012
1101	3	0.2739851257383137
1101	4	0.1517210251180927
1101	5	0.18934841602713703
1101	6	0.21776181215992096
1101	7	0.26131539158096473
1101	8	0.26311754404258636
1101	9	0.3747237074703804
1101	10-11	0.3252026826925132
1101	12-13	0.31170967692925444
1101	14-15	0.44035735960503786
1101	16-17	0.4497428612699039
1101	18-19	0.45183556613483233
1101	20-21	0.4751591006198055
1101	22-23	0.5326556976045325
1101	24-25	0.4648115574025695
1101	26-27	0.44151195834393775
1101	28-29	0.4453346136316938
1101	30-31	0.5278149139941632
1101	32-33	0.4902294786783514
1101	34-35	0.5183154580668017
1101	36-37	0.5465864733416339
1101	38-39	0.6061466577137651
1101	40-41	0.6398490498149982
1101	42-43	0.6572642426293314
1101	44-45	0.7237055450404313
1101	46-47	0.6561994998616569
1101	48-49	0.6934306977170195
1101	50-51	0.7158591502344649
1101	52-53	0.7037224695017912
1101	54-55	0.7411602162698543
1101	56-57	0.7358357014290675
1101	58-59	0.7031684503325977
1101	60-61	0.6412818331340233
1101	62-63	0.6006360419056023
1101	64-65	0.6718003469442237
1101	66-67	0.6761923888447754
1101	68-69	0.6653306719245791
1101	70-71	0.6196219978009694
1101	72-73	0.6680676473336717
1101	74-75	0.7079356995558257
1101	76-77	0.6355946565602224
1101	78-79	0.5833987810564878
1101	80-81	0.5947690712254641
1101	82-83	0.6401419884944275
1101	84-85	0.6012958638120836
1101	86-87	0.5779792268284467
1101	88-89	0.5867616402466354
1101	90-91	0.518897830349573
1101	92-93	0.5236933214194011
1101	94-95	0.6275925779660767
1101	96-97	0.6917120510734165
1101	98-99	0.6767257909494617
1101	100	0.6820492899980621
1102	1	0.13149772575396668
1102	2	0.1526826052725454
1102	3	0.1949602797105996
1102	4	0.1090646764642429
1102	5	0.10591941904991842
1102	6	0.13009305552517958
1102	7	0.16436717668815248
1102	8	0.21698085504232267
1102	9	0.24977818463529644
1102	10-11	0.25797302750255113
1102	12-13	0.28368799245045295
1102	14-15	0.3705680755675118
1102	16-17	0.3862278294578374
1102	18-19	0.3467425814430172
1102	20-21	0.27274846005345665
1102	22-23	0.3257652786935239
1102	24-25	0.3551904575905951
1102	26-27	0.3705345609902082
1102	28-29	0.29741200797883494
1102	30-31	0.2827118456496578
1102	32-33	0.27790003926931206
1102	34-35	0.3174070967297453
1102	36-37	0.30104342470188783
1102	38-39	0.25069137848215206
1102	40-41	0.29477840642707065
1102	42-43	0.28571471362378986
1102	44-45	0.2708967158311424
1102	46-47	0.2702223453481736
1102	48-49	0.267779735491402
1102	50-51	0.31821058403274094
1102	52-53	0.24132811079665828
1102	54-55	0.22634335251712656
1102	56-57	0.3175664355236165
1102	58-59	0.30264479422215373
1102	60-61	0.29675885235078425
1102	62-63	0.30201870496127015
1102	64-65	0.3537170735500439
1102	66-67	0.34645134907216857
1102	68-69	0.3419945555647814
1102	70-71	0.30053466087976943
1102	72-73	0.2811923539016874
1102	74-75	0.22026277994520171
1102	76-77	0.2854809841910537
1102	78-79	0.3237997218890314
1102	80-81	0.2516834272477837
1102	82-83	0.2705676990334709
1102	84-85	0.23796469676804577
1102	86-87	0.2753254222154098
1102	88-89	0.337511230309147
1102	90-91	0.30710254700124295
1102	92-93	0.3491440907604755
1102	94-95	0.3034400557808752
1102	96-97	0.2882861069451863
1102	98-99	0.34696426008447645
1102	100	0.4423899139356351
1103	1	0.1777572660073119
1103	2	0.24308281393900444
1103	3	0.2407761769196881
1103	4	0.16149938314043055
1103	5	0.16308087472530275
1103	6	0.20701658607099915
1103	7	0.18942847246152184
1103	8	0.24175727573205563
1103	9	0.28531680494172207
1103	10-11	0.2965023825644124
1103	12-13	0.2934510879720591
1103	14-15	0.4003257276161847
1103	16-17	0.40262697211084486
1103	18-19	0.34633726952067434
1103	20-21	0.36334554099109084
1103	22-23	0.3829500959402594
1103	24-25	0.3565592642225681
1103	26-27	0.3730313005767272
1103	28-29	0.37408627282647444
1103	30-31	0.390617356031953
1103	32-33	0.3733663067479611
1103	34-35	0.36213634065403966
1103	36-37	0.3515534100725546
1103	38-39	0.35270047361818513
1103	40-41	0.3452217092542753
1103	42-43	0.3586186930293209
1103	44-45	0.37522110145583554
1103	46-47	0.3442964446235166
1103	48-49	0.29242982224674563
1103	50-51	0.33495085098972055
1103	52-53	0.3366419610331164
1103	54-55	0.3611144431396056
1103	56-57	0.28888001358642157
1103	58-59	0.32985943716435173
1103	60-61	0.2883060461270759
1103	62-63	0.33458178687419604
1103	64-65	0.34873516175698427
1103	66-67	0.3738814628180691
1103	68-69	0.3439294833820554
1103	70-71	0.3541587663924233
1103	72-73	0.367871722005205
1103	74-75	0.3298312713093594
1103	76-77	0.3403103653362223
1103	78-79	0.29803463042735245
1103	80-81	0.23657892390798452
1103	82-83	0.27607036973737564
1103	84-85	0.3213934042834481
1103	86-87	0.31121582696030714
1103	88-89	0.3484244862998054
1103	90-91	0.3772931819596792
1103	92-93	0.39781962575179364
1103	94-95	0.34228966850046305
1103	96-97	0.32822486787465976
1103	98-99	0.3603658636408724
1103	100	0.406775085008114
1104	1	0.18691704327854097
1104	2	0.2079168447173103
1104	3	0.2175166706880418
1104	4	0.12059735431270724
1104	5	0.1282675971875662
1104	6	0.16522318855004414
1104	7	0.15157365442094317
1104	8	0.17959508291971105
1104	9	0.2678001626572737
1104	10-11	0.2437914196367359
1104	12-13	0.2174936604666442
1104	14-15	0.3373696950007101
1104	16-17	0.3374481880466149
1104	18-19	0.31404159821571653
1104	20-21	0.35629964570990325
1104	22-23	0.2940706575252179
1104	24-25	0.3502164841955704
1104	26-27	0.37643970847430097
1104	28-29	0.3636988790025484
1104	30-31	0.39344059058893066
1104	32-33	0.38508338224212935
1104	34-35	0.3796858705874726
1104	36-37	0.46389478386903704
1104	38-39	0.4273310545955198
1104	40-41	0.5177917089140607
1104	42-43	0.5315793752779285
1104	44-45	0.5218625920601738
1104	46-47	0.5967000781416942
1104	48-49	0.5911574104480195
1104	50-51	0.5220798726388693
1104	52-53	0.5816866995763021
1104	54-55	0.5729483264905113
1104	56-57	0.5586074997225694
1104	58-59	0.5399553783748416
1104	60-61	0.5747750466827668
1104	62-63	0.6236092197096781
1104	64-65	0.6646771660890778
1104	66-67	0.6658324942427853
1104	68-69	0.6421796336445951
1104	70-71	0.6262836487340238
1104	72-73	0.5954780681874041
1104	74-75	0.6203437516051196
1104	76-77	0.6034971785230354
1104	78-79	0.5690917982337425
1104	80-81	0.5373687945120977
1104	82-83	0.5154028638686299
1104	84-85	0.5679878315280291
1104	86-87	0.5745908935861408
1104	88-89	0.5595008196671571
1104	90-91	0.5419724139763673
1104	92-93	0.521532754728085
1104	94-95	0.5580785751562374
1104	96-97	0.5368459681366247
1104	98-99	0.5037832305876577
1104	100	0.5333789249246479
1105	1	-0.03089439732517718
1105	2	0.07571529196828664
1105	3	0.05018171662622706
1105	4	-0.06440231434812205
1105	5	-0.10645046787495716
1105	6	-0.10521028982078917
1105	7	-0.39282249815883574
1105	8	-0.2745069827284681
1105	9	-0.19846100963111013
1105	10-11	-0.240482980543959
1105	12-13	-0.11072658328102136
1105	14-15	-0.1272369498935646
1105	16-17	-0.0628953985281484
1105	18-19	-0.10770875385383505
1105	20-21	-0.09449700814614204
1105	22-23	-0.07995184855771953
1105	24-25	0.004670411223933968
1105	26-27	-0.03616880510348608
1105	28-29	0.002418705887372141
1105	30-31	-0.013699699109054109
1105	32-33	-0.009059651499306653
1105	34-35	-0.09773978366860803
1105	36-37	-0.03665799741970943
1105	38-39	-0.014993725098626953
1105	40-41	-0.08446502308251524
1105	42-43	-0.06101864647110489
1105	44-45	-0.03636237370433548
1105	46-47	-0.012006231492847519
1105	48-49	-0.061972374237029726
1105	50-51	-0.05440326913647198
1105	52-53	-0.02335961331265679
1105	54-55	-0.06383047737855208
1105	56-57	0.004679794806641269
1105	58-59	-0.03199674363568761
1105	60-61	0.038156257080359524
1105	62-63	0.09164019419725378
1105	64-65	0.06136960319448548
1105	66-67	0.027183879257854926
1105	68-69	-0.04079574351825954
1105	70-71	-0.022317024422264353
1105	72-73	0.0038204598594404615
1105	74-75	-0.018938657962706884
1105	76-77	0.04124479383514057
1105	78-79	0.027195087928610917
1105	80-81	-0.1087202063112187
1105	82-83	-0.05521730937838498
1105	84-85	-0.04585640367351118
1105	86-87	-0.051857847897487375
1105	88-89	-0.09325052711380977
1105	90-91	-0.03523811268637189
1105	92-93	-0.03256660469441641
1105	94-95	-0.04095341139034758
1105	96-97	-0.014940435716212619
1105	98-99	-0.014773497627146526
1105	100	-0.04997456053789051
1106	1	0.07471167373735454
1106	2	0.07133684344238134
1106	3	0.07590963620722846
1106	4	0.0495928270902013
1106	5	0.06880354005234324
1106	6	0.10996413305810648
1106	7	0.04563317353868257
1106	8	0.08171485415918056
1106	9	0.13930747850552905
1106	10-11	0.16492330746633854
1106	12-13	0.13414861706105086
1106	14-15	0.16375034741768957
1106	16-17	0.1900702593459087
1106	18-19	0.14459235470965126
1106	20-21	0.13225378007111743
1106	22-23	0.09384416456467903
1106	24-25	0.1370035986492013
1106	26-27	0.12398633201068066
1106	28-29	0.11353493799091297
1106	30-31	0.1475138345317646
1106	32-33	0.11785301906392931
1106	34-35	0.0793248436311913
1106	36-37	0.023274505218950026
1106	38-39	0.12562565705872686
1106	40-41	0.07150949400119799
1106	42-43	0.10691655168636771
1106	44-45	0.07187615558095217
1106	46-47	0.049077850966611436
1106	48-49	0.09645028555924995
1106	50-51	0.1548807389050353
1106	52-53	0.12880392019142306
1106	54-55	0.15260962156867208
1106	56-57	0.07228827632835788
1106	58-59	0.05586327888917708
1106	60-61	0.12383059445745204
1106	62-63	0.126624951067285
1106	64-65	0.12507870369135787
1106	66-67	0.0969996425734152
1106	68-69	0.10658636493535312
1106	70-71	0.09067626589794742
1106	72-73	0.16198585011204614
1106	74-75	0.10624567765609072
1106	76-77	0.15172402542244612
1106	78-79	0.1327607406870328
1106	80-81	0.14065787462931212
1106	82-83	0.13904432099867847
1106	84-85	0.14145810446267149
1106	86-87	0.1626522267569328
1106	88-89	0.20619754787296785
1106	90-91	0.2106823331520573
1106	92-93	0.24125465679745162
1106	94-95	0.20025602974636314
1106	96-97	0.1670368454164617
1106	98-99	0.12917848430149093
1106	100	0.11789315312773851
1107	1	-0.1300650671699941
1107	2	-0.1067637559887018
1107	3	-0.04155075915978301
1107	4	-0.11415464504909068
1107	5	-0.13302356174424546
1107	6	-0.15546846099822886
1107	7	-0.1458396400885391
1107	8	-0.07677395638943807
1107	9	-0.12013386338927035
1107	10-11	-0.08599358664555012
1107	12-13	-0.02784448596042921
1107	14-15	-0.060273949920613745
1107	16-17	-0.10061846399462837
1107	18-19	-0.06677450038623078
1107	20-21	-0.07795575406274224
1107	22-23	-0.021585192976367296
1107	24-25	-0.0627599223380031
1107	26-27	-0.08074203899782617
1107	28-29	-0.028594852674125093
1107	30-31	-0.051094819554464266
1107	32-33	-0.07884932751635176
1107	34-35	-0.02041683976932518
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2216	62-63	-1.0039603793719536
2216	64-65	-0.8691489149506282
2216	66-67	-0.7795942202591348
2216	68-69	-0.8746440998974094
2216	70-71	-0.8012561539181391
2216	72-73	-0.9080601892208193
2216	74-75	-0.8530068267090058
2216	76-77	-0.834264774893569
2216	78-79	-0.7672898616523582
2216	80-81	-0.7633070850861827
2216	82-83	-0.7681921988136153
2216	84-85	-0.720454501131826
2216	86-87	-0.7900721323921687
2216	88-89	-0.7345839031601997
2216	90-91	-0.7219902038512203
2216	92-93	-0.9395200743962491
2216	94-95	-0.87686012541214
2216	96-97	-1.0810688744361627
2216	98-99	-1.1372910651355994
2216	100	-1.1293913418604014
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24.0
3	230.0
4	130.0
5	235.0
6	273.0
7	510.0
8	841.0
9	1150.0
10	1696.0
11	2606.0
12	4331.0
13	6218.0
14	6349.0
15	7842.0
16	10541.0
17	17162.0
18	21106.0
19	10591.0
20	6366.0
21	5715.0
22	5645.0
23	5724.0
24	6277.0
25	7611.0
26	9296.0
27	11821.0
28	14680.0
29	18358.0
30	23072.0
31	29106.0
32	36866.0
33	47780.0
34	68912.0
35	119654.0
36	205489.0
37	474384.0
38	789130.0
39	364748.0
40	7376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.952350957892236	20.850658697212083	14.563903035276072	23.63308730961961
2	14.372139438984274	21.95017118150346	39.84884109377427	23.828848285737998
3	15.550014745703638	28.95524242745348	31.45141556627119	24.04332726057169
4	11.075661164903106	18.698080510955865	39.08282094755621	31.14343737658482
5	12.202932533847976	37.92956556709059	34.854767016547896	15.012734882513529
6	27.947928480389134	39.16232772799908	18.773706350844417	14.116037440767371
7	24.73818485900134	33.888703297451535	24.12380391047069	17.249307933076437
8	25.218131408667382	33.79154795316287	21.66589711236273	19.32442352580702
9	26.251135713206615	15.2644110568995	22.11167119533416	36.37278203455973
10-11	23.513721117775855	26.651162097925607	29.27207964780656	20.56303713649198
12-13	25.0569292868253	25.284008094150888	29.170817649674767	20.488244969349044
14-15	22.60453349050682	25.727867157195472	28.056595222238062	23.611004130059644
16-17	20.8015209513819	28.932823228766154	28.193774482997814	22.071881336854133
18-19	20.419027637993146	26.832897488983313	32.010409197202364	20.73766567582117
20-21	22.272171143203064	25.990288721170973	31.47890605550579	20.258634080120178
22-23	21.54245591248949	26.313241711628883	31.600381046652682	20.543921329228944
24-25	21.23038805980312	26.10528613814364	31.618418073710426	21.045907728342815
26-27	21.058511042354613	26.43966808782816	31.622790247074427	20.879030622742796
28-29	20.996778702454797	26.183963559909344	31.933976486379116	20.88528125125674
30-31	21.482265785119463	26.486610820969204	31.31587772277565	20.715245671135683
32-33	20.726609213746144	26.494329183955738	31.83538674327056	20.943674859027556
34-35	21.256844682495892	25.88219347323762	31.575709588205093	21.285252256061398
36-37	21.63356361941025	26.379881166511364	30.95194676389084	21.03460845018754
38-39	21.286238071011013	26.32387866067209	31.211505876800505	21.178377391516392
40-41	21.318151689475663	26.191676410538488	31.021111663175187	21.469060236810662
42-43	21.05715372719311	26.579151413842144	31.13220996511249	21.231484893852258
44-45	21.48378203897894	26.348148996120152	30.68192505595079	21.486143908950126
46-47	21.70560912225258	26.758613234781865	30.09202388063004	21.44375376233551
48-49	21.517956011538015	26.58179554157421	30.02187847636265	21.878369970525128
50-51	21.9016968302925	26.71095369969434	29.308256261909143	22.079093208104016
52-53	21.674843095967223	27.097713720570386	29.355501897147214	21.871941286315177
54-55	21.871133368016658	27.125848331742798	29.179888353744367	21.823129946496188
56-57	21.822138395720195	27.075546412565693	29.155954885189423	21.946360306524692
58-59	21.69145073960701	26.39040968513028	29.65856238900788	22.25957718625483
60-61	22.261742668698297	26.84261067303136	29.10991542610269	21.785731232167656
62-63	21.689494413461315	26.798831412284642	29.63874212980005	21.872932044453997
64-65	21.831588892033306	26.49619442984537	29.630252208124364	22.04196446999696
66-67	21.914790802818164	26.75752719826149	29.312032771753344	22.015649227167003
68-69	22.122467982265103	26.730566311059455	29.326519508534766	21.820446198140676
70-71	22.26947439020446	26.53470246880165	29.21147891483715	21.984344226156736
72-73	21.850826172392406	26.73168520868952	29.414226698172325	22.00326192074575
74-75	21.95378330070644	26.558111328623713	29.186739296961445	22.3013660737084
76-77	22.058019343469358	26.809391459918285	29.030268908013618	22.102320288598744
78-79	21.964124493764523	26.765789131552815	29.14792853326456	22.1221578414181
80-81	21.93878061261944	26.91523101163809	28.952806110235752	22.19318226550672
82-83	21.605253504307985	26.913783256774344	29.23391644133045	22.247046797587224
84-85	21.812188713057566	26.626362896075296	28.962646737209614	22.59880165365752
86-87	21.930162023866316	26.895417090277245	28.825847217617888	22.348573668238547
88-89	21.484206519280562	26.701554526963722	29.139193753379256	22.675045200376456
90-91	21.67178090668124	26.743549429390402	28.812247280260568	22.772422383667795
92-93	21.538356782337072	26.873600932853286	29.121002817285024	22.467039467524618
94-95	21.54713061122528	26.417842039883038	29.12838892834816	22.906638420543523
96-97	21.551709830006445	26.819892799159074	29.023382890847927	22.60501447998655
98-99	21.215421319218006	26.601379870337126	29.12022626741329	23.062972543031577
100	21.40175123150089	26.513559808917876	29.03405645221351	23.050632507367727
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2437.0
1	1575.5
2	3221.0
3	4553.0
4	2109.0
5	1002.0
6	2015.5
7	4780.0
8	8360.5
9	9133.5
10	5693.0
11	2551.5
12	1951.5
13	2046.0
14	2282.5
15	2427.5
16	2643.5
17	2934.5
18	3174.5
19	3563.0
20	4089.5
21	4826.0
22	5736.0
23	7123.0
24	9116.5
25	11238.0
26	14227.0
27	18242.5
28	22945.0
29	28369.0
30	33220.0
31	38100.5
32	43192.0
33	47328.0
34	51535.5
35	56158.5
36	60739.0
37	65216.0
38	69912.0
39	73862.0
40	78430.5
41	82534.5
42	83689.5
43	86481.0
44	87746.5
45	85776.0
46	85349.0
47	85009.5
48	82702.0
49	81628.5
50	82559.0
51	80386.0
52	74159.0
53	68491.5
54	64166.5
55	59483.0
56	53042.0
57	46107.0
58	42728.5
59	40170.5
60	35883.5
61	30743.5
62	25314.5
63	19687.0
64	14763.5
65	11143.0
66	8332.0
67	6144.0
68	4349.5
69	3015.5
70	2183.0
71	1634.0
72	1117.0
73	746.5
74	579.5
75	422.0
76	269.0
77	185.0
78	108.5
79	65.0
80	38.0
81	14.5
82	7.0
83	4.5
84	5.5
85	4.5
86	2.5
87	3.0
88	3.5
89	2.0
90	0.5
91	0.5
92	0.5
93	1.5
94	5.0
95	4.5
96	3.5
97	4.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008809091663492698
2	0.0
3	3.4044798699488693E-4
4	0.0022554679138411258
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.383399756154129E-5
24-25	4.2555998374360866E-5
26-27	2.766139894333456E-4
28-29	6.596179748025934E-4
30-31	0.001574571939851352
32-33	0.004042819845564282
34-35	0.005085441805736123
36-37	0.0036810938593822146
38-39	0.002319301911402667
40-41	0.0032980898740129667
42-43	2.9789198862052604E-4
44-45	5.106719804923303E-4
46-47	0.003170421878889884
48-49	0.0020001319235949605
50-51	9.575099634231194E-4
52-53	4.2555998374360866E-5
54-55	5.319499796795108E-4
56-57	7.660079707384955E-4
58-59	6.808959739897739E-4
60-61	2.766139894333456E-4
62-63	0.0
64-65	0.0
66-67	7.021739731769542E-4
68-69	6.170619764282326E-4
70-71	0.0017447959333487955
72-73	1.0638999593590215E-4
74-75	0.0019150199268462389
76-77	3.4044798699488693E-4
78-79	0.0010426219601718412
80-81	7.447299715513151E-4
82-83	9.787879626102997E-4
84-85	0.0010000659617974803
86-87	2.5533599024616516E-4
88-89	0.0012979579504180062
90-91	0.0014256259455410888
92-93	0.0027661398943334557
94-95	0.0010213439609846606
96-97	0.002127799918718043
98-99	0.002510803904087291
100	0.0029789198862052602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2349845.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.438819549807974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2427763314077	41.713706994634734
2	14.79015883035119	16.39897893017677
3	4.531193572976335	7.536120685124541
4	1.8197470511647982	4.035385135832817
5	0.9555202601717168	2.6486457639922687
6	0.5678616656798984	1.888894824772876
7	0.36979846042679454	1.4350833082178505
8	0.25706090499839396	1.1400922500413035
9	0.202321741526003	1.0094830667516672
>10	1.12721649279822	11.537144881760584
>50	0.09006534152876636	3.435577899752309
>100	0.04412968022681444	4.280158337732147
>500	0.0014587024329727723	0.5304138156968704
>1k	3.838690613084948E-4	0.5175173796386218
>5k	1.5354762452339795E-4	0.6507805522390433
>10k+	1.5354762452339795E-4	1.2420161736355475
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16640	0.7081318129493647	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12541	0.5336947756128595	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8388	0.3569597143641389	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6902	0.2937215007798387	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3096	0.13175337096702122	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2888	0.12290172330515416	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2727	0.11605020756688207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	4.255599837436086E-5	0.0
16-17	0.0	0.0	0.0	4.255599837436086E-5	0.0
18-19	0.0	0.0	0.0	6.383399756154129E-5	0.0
20-21	0.0	0.0	0.0	1.4894599431026302E-4	0.0
22-23	0.0	0.0	0.0	5.106719804923303E-4	0.0
24-25	0.0	0.0	0.0	0.0012554019520436455	0.0
26-27	0.0	0.0	0.0	0.0019788539244077804	0.0
28-29	4.255599837436086E-5	0.0	0.0	0.003595981862633493	0.0
30-31	4.255599837436086E-5	0.0	0.0	0.00838353167974909	0.0
32-33	4.255599837436086E-5	0.0	0.0	0.016384059374128932	0.0
34-35	4.255599837436086E-5	0.0	0.0	0.029044468890501288	0.0
36-37	4.255599837436086E-5	0.0	0.0	0.04883300813457909	0.0
38-39	4.255599837436086E-5	0.0	0.0	0.07389849117707764	0.0
40-41	6.383399756154129E-5	0.0	0.0	0.10249612208464814	0.0
42-43	8.511199674872172E-5	0.0	0.0	0.12834889109707237	0.0
44-45	8.511199674872172E-5	0.0	0.0	0.1626490257868072	0.0
46-47	8.511199674872172E-5	0.0	0.0	0.2036730082196911	0.0
48-49	8.511199674872172E-5	0.0	0.0	0.24635667458917504	0.0
50-51	8.511199674872172E-5	0.0	0.0	0.29223204083673604	0.0
52-53	8.511199674872172E-5	0.0	0.0	0.3394479210330894	0.0
54-55	8.511199674872172E-5	0.0	0.0	0.3882596511684813	0.0
56-57	8.511199674872172E-5	0.0	0.0	0.4502850187991123	0.0
58-59	8.511199674872172E-5	0.0	0.0	0.5177149982232871	0.0
60-61	8.511199674872172E-5	0.0	0.0	0.5935285093272109	0.0
62-63	8.511199674872172E-5	0.0	0.0	0.6719379363319709	0.0
64-65	8.511199674872172E-5	0.0	0.0	0.7564115931050771	0.0
66-67	8.511199674872172E-5	0.0	0.0	0.8599716151490844	0.0
68-69	8.511199674872172E-5	0.0	0.0	0.9754473167379125	0.0
70-71	8.511199674872172E-5	0.0	0.0	1.1047111618000336	0.0
72-73	8.511199674872172E-5	0.0	0.0	1.2435501064963859	0.0
74-75	8.511199674872172E-5	0.0	0.0	1.3908364168700489	0.0
76-77	8.511199674872172E-5	0.0	0.0	1.54840000085112	0.0
78-79	1.2766799512308258E-4	0.0	0.0	1.7283905959754793	0.0
80-81	1.2766799512308258E-4	0.0	0.0	1.9306592562488163	0.0
82-83	1.2766799512308258E-4	0.0	0.0	2.1533760737410343	0.0
84-85	1.2766799512308258E-4	0.0	0.0	2.3816677270202926	0.0
86-87	1.2766799512308258E-4	0.0	0.0	2.6257689336956265	0.0
88	1.2766799512308258E-4	0.0	0.0	2.8209520202396328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6000	0.0	55.46526	1
GTATCAA	12140	0.0	45.76546	1
ATCAACG	15500	0.0	35.567978	3
TCAACGC	15620	0.0	35.23455	4
CAACGCA	16115	0.0	34.239754	5
TATCAAC	16175	0.0	34.112743	2
AACGCAG	16645	0.0	33.234222	6
ACGCAGA	18530	0.0	29.701221	7
CGCAGAG	18815	0.0	29.126425	8
GCAGAGT	20050	0.0	27.051062	9
TACCTGG	1705	0.0	25.636068	2
GTACATG	11945	0.0	23.807198	1
TACATGG	11750	0.0	23.399729	2
GTACCTG	1950	0.0	22.17646	1
GAGTACT	13390	0.0	22.095713	12-13
ACATGGG	12130	0.0	22.085484	3
AGAGTAC	19125	0.0	21.969942	10-11
GCGTATC	150	3.2908498E-5	21.935415	1
CAGAGTA	19545	0.0	21.786394	10-11
AGTACTT	13495	0.0	21.175005	12-13
>>END_MODULE