FastQCFastQC Report
Wed 25 May 2016
SRR2431378_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431378_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496194
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157430.35014058557081834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.046.31851
GTATCAA74550.038.9614871
TCAACGC82450.033.6322564
ATCAACG82600.033.571183
CAACGCA89500.031.0880375
AACGCAG92900.029.9502666
TATCAAC99100.028.4084682
ACGCAGA103900.026.281817
CGCAGAG104800.025.9664138
GTACATG165100.024.5388871
TACATGG161300.023.7183632
ACATGGG165350.022.7679023
GCAGAGT124950.021.3275749
CATGGGT46200.021.2617474
CATGGGG96000.018.6529884
AGAGTAC109950.017.61151310-11
CAGAGTA122100.017.55269810-11
GAGTACA119250.016.3956851
GTATAGA18650.015.6245511
GGACCGT2851.7503373E-514.8419966
Produced by FastQC (version 0.11.5)