##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431378_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4496194 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44814436387754 33.0 31.0 34.0 29.0 34.0 2 31.54084832638449 34.0 31.0 34.0 29.0 34.0 3 31.60601433123215 34.0 31.0 34.0 28.0 34.0 4 34.84516215270071 37.0 35.0 37.0 32.0 37.0 5 34.94789392984377 37.0 35.0 37.0 32.0 37.0 6 35.042263078505954 37.0 35.0 37.0 32.0 37.0 7 34.987729844397286 37.0 35.0 37.0 32.0 37.0 8 34.98757282270294 37.0 35.0 37.0 32.0 37.0 9 36.56786184048108 39.0 38.0 39.0 33.0 39.0 10-11 36.57981506136078 39.0 38.0 39.0 33.0 39.0 12-13 36.52826757475323 39.0 38.0 39.0 33.0 39.0 14-15 37.9533000577822 41.0 39.0 41.0 33.0 41.0 16-17 37.95803984436615 41.0 39.0 41.0 33.0 41.0 18-19 37.988820211049614 41.0 39.0 41.0 33.0 41.0 20-21 38.03233790623803 41.0 39.0 41.0 33.0 41.0 22-23 38.02821252819607 41.0 39.0 41.0 33.0 41.0 24-25 38.03791695820954 41.0 39.0 41.0 33.0 41.0 26-27 38.03230143094359 41.0 39.0 41.0 33.0 41.0 28-29 38.001737803128606 41.0 39.0 41.0 32.5 41.0 30-31 37.93926385293873 41.0 38.0 41.0 32.0 41.0 32-33 37.87135586231377 41.0 38.0 41.0 32.0 41.0 34-35 37.81210374819236 41.0 38.0 41.0 32.0 41.0 36-37 37.724862072232646 40.0 38.0 41.0 32.0 41.0 38-39 37.636624442806514 40.0 38.0 41.0 31.0 41.0 40-41 37.56020714408675 40.0 38.0 41.0 31.0 41.0 42-43 37.45455845544031 40.0 38.0 41.0 31.0 41.0 44-45 37.33704361955912 40.0 38.0 41.0 30.5 41.0 46-47 37.22541576275401 40.0 38.0 41.0 30.0 41.0 48-49 37.10925195843418 40.0 37.0 41.0 30.0 41.0 50-51 36.98863338637078 40.0 37.0 41.0 30.0 41.0 52-53 36.84171145640068 40.0 37.0 41.0 30.0 41.0 54-55 36.6925327287924 40.0 36.5 41.0 30.0 41.0 56-57 36.5111196269556 40.0 36.0 41.0 30.0 41.0 58-59 36.30718859106169 39.5 35.5 41.0 30.0 41.0 60-61 36.14994537602247 39.0 35.0 41.0 29.5 41.0 62-63 35.99955217679664 39.0 35.0 41.0 29.0 41.0 64-65 35.75744162729633 39.0 35.0 41.0 29.0 41.0 66-67 35.46273837383352 38.5 35.0 40.5 29.0 41.0 68-69 35.154370785602225 37.5 35.0 40.0 28.0 41.0 70-71 34.89427824955952 37.0 35.0 40.0 28.0 41.0 72-73 34.640664637691344 37.0 34.5 39.5 28.0 41.0 74-75 34.28691155230402 36.0 34.0 39.0 28.0 41.0 76-77 33.91999744672939 36.0 34.0 39.0 28.0 40.5 78-79 33.564067075397546 35.0 34.0 37.5 27.0 39.5 80-81 33.20257399925359 35.0 34.0 37.0 27.0 39.0 82-83 32.849713891349 35.0 34.0 37.0 26.0 39.0 84-85 32.54285936060588 35.0 33.5 36.0 26.0 38.0 86-87 32.26021652980276 35.0 33.0 36.0 26.0 37.0 88-89 31.996250161803516 35.0 33.0 36.0 26.0 37.0 90-91 31.77925674470452 35.0 33.0 35.0 25.0 36.5 92-93 31.569232666561987 35.0 33.0 35.0 25.0 36.0 94-95 31.36041427482889 35.0 33.0 35.0 24.5 36.0 96-97 31.173983707108725 35.0 33.0 35.0 24.0 36.0 98-99 31.057202046886765 35.0 33.0 35.0 24.0 36.0 100 31.01717763957694 35.0 33.0 35.0 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2635100923831679 1101 2 0.29671402114318113 1101 3 0.2841683218675506 1101 4 0.3282074369705086 1101 5 0.22986995033196678 1101 6 0.29241365157330534 1101 7 0.3286183960028808 1101 8 0.3263721433406417 1101 9 0.3268107832411431 1101 10-11 0.3234190874736953 1101 12-13 0.33300226903667607 1101 14-15 0.3738054902020096 1101 16-17 0.3822662014539162 1101 18-19 0.35086565569737616 1101 20-21 0.34981146265437246 1101 22-23 0.3502459019464297 1101 24-25 0.32605743674162113 1101 26-27 0.38123447926762566 1101 28-29 0.3950971642848202 1101 30-31 0.44381009053474685 1101 32-33 0.4103916354121324 1101 34-35 0.4518383902506571 1101 36-37 0.4258242634567537 1101 38-39 0.38697253745782234 1101 40-41 0.4647486212203873 1101 42-43 0.4092427091114743 1101 44-45 0.3942963832484381 1101 46-47 0.35875219965368643 1101 48-49 0.44018219948766557 1101 50-51 0.3609620595564351 1101 52-53 0.36458696895748943 1101 54-55 0.39751627861701166 1101 56-57 0.3084158035404485 1101 58-59 0.28705877348423314 1101 60-61 0.365457314571195 1101 62-63 0.34653306904949943 1101 64-65 0.32711196826373623 1101 66-67 0.2943842136056958 1101 68-69 0.30518401837199605 1101 70-71 0.2974795198128888 1101 72-73 0.3420868018824308 1101 74-75 0.3680691172331336 1101 76-77 0.3178035841698019 1101 78-79 0.374118721643093 1101 80-81 0.29753242865833585 1101 82-83 0.3228867602758143 1101 84-85 0.32479131466961775 1101 86-87 0.3005457282407704 1101 88-89 0.33035706745822324 1101 90-91 0.3553687445427691 1101 92-93 0.39427426640539665 1101 94-95 0.3635864179901098 1101 96-97 0.3678603385410355 1101 98-99 0.3781951146270579 1101 100 0.42005271317951554 1102 1 0.08816519798462252 1102 2 0.11691744349501576 1102 3 0.09827525929504688 1102 4 0.05246232619617075 1102 5 0.02233263794003193 1102 6 0.08152093770057434 1102 7 0.12264629077195366 1102 8 0.09618147430403212 1102 9 0.1595000832521194 1102 10-11 0.15799978054330666 1102 12-13 0.07706656503003018 1102 14-15 0.1567531464240517 1102 16-17 0.1495914255465749 1102 18-19 0.15363333128894396 1102 20-21 0.12700140336328758 1102 22-23 0.1078580180204014 1102 24-25 0.19257667920803812 1102 26-27 0.17997755302884855 1102 28-29 0.1573854201370608 1102 30-31 0.18344885576155434 1102 32-33 0.18201488744080763 1102 34-35 0.20570592557395173 1102 36-37 0.22306610888511358 1102 38-39 0.18171594900299937 1102 40-41 0.16938672240154062 1102 42-43 0.19721497747324435 1102 44-45 0.2236915316520296 1102 46-47 0.21681971763294428 1102 48-49 0.19489146574449734 1102 50-51 0.22877315888250394 1102 52-53 0.23314004075992756 1102 54-55 0.22395157237079388 1102 56-57 0.2875629506001829 1102 58-59 0.2382094979004279 1102 60-61 0.18130456708987452 1102 62-63 0.2294248271816386 1102 64-65 0.2562712591721379 1102 66-67 0.18768754056539905 1102 68-69 0.14243863227644482 1102 70-71 0.19324838159011648 1102 72-73 0.16309465945282398 1102 74-75 0.14856276089108178 1102 76-77 0.16856182424868393 1102 78-79 0.14286444249042773 1102 80-81 0.16457335630889247 1102 82-83 0.1997015909926887 1102 84-85 0.17679615644179592 1102 86-87 0.1748502996038397 1102 88-89 0.1498025226266506 1102 90-91 0.22149069667933574 1102 92-93 0.19524356081291927 1102 94-95 0.2055855471503243 1102 96-97 0.19587797122736106 1102 98-99 0.18885068233151614 1102 100 0.2242233747327269 1103 1 0.062257445770832476 1103 2 0.044595732552576095 1103 3 0.11913646264735434 1103 4 0.14658026974455396 1103 5 0.1033354644738651 1103 6 0.13931861322239314 1103 7 0.12636792660370588 1103 8 0.1864122420143417 1103 9 0.17614708277773872 1103 10-11 0.19810928402210948 1103 12-13 0.18638221770238772 1103 14-15 0.2436048719933126 1103 16-17 0.20773540087864717 1103 18-19 0.2479556055615575 1103 20-21 0.19674513755050782 1103 22-23 0.2584933951551278 1103 24-25 0.20417110125104188 1103 26-27 0.26830045981252937 1103 28-29 0.21389203185432848 1103 30-31 0.18504950408539855 1103 32-33 0.19764507600817183 1103 34-35 0.2374683089547176 1103 36-37 0.2303260511684826 1103 38-39 0.21696529672595943 1103 40-41 0.1712061306083683 1103 42-43 0.1880717828970262 1103 44-45 0.14784391740472103 1103 46-47 0.10872733148494262 1103 48-49 0.14431088976410678 1103 50-51 0.1076602358907337 1103 52-53 0.10391842469414314 1103 54-55 0.18388347860103238 1103 56-57 0.060515640748825206 1103 58-59 0.08919646224261868 1103 60-61 0.10625362029986007 1103 62-63 0.05899249598302703 1103 64-65 0.07172483878946423 1103 66-67 0.06810470138134406 1103 68-69 0.07383539461040556 1103 70-71 0.05124752177037806 1103 72-73 0.002194813627397707 1103 74-75 0.05728676863933657 1103 76-77 0.0942754252132687 1103 78-79 0.07287773510586959 1103 80-81 0.10912733717543688 1103 82-83 0.10392305881368458 1103 84-85 0.10953838841523122 1103 86-87 0.10336414566646113 1103 88-89 0.1999606998088197 1103 90-91 0.12251704471854907 1103 92-93 0.1539519040292383 1103 94-95 0.1615242825235086 1103 96-97 0.1510314045765071 1103 98-99 0.15143182164207758 1103 100 0.13547337473272592 1104 1 0.0717206397880581 1104 2 0.07199018810471358 1104 3 0.09787271687682164 1104 4 0.04788309003062352 1104 5 0.010231948248090816 1104 6 0.0701946898142154 1104 7 0.12256875903987918 1104 8 0.14776254768951702 1104 9 0.09563767221175823 1104 10-11 0.12241507454810119 1104 12-13 0.10736977441570872 1104 14-15 0.12333728605821648 1104 16-17 0.12396290779959429 1104 18-19 0.11130411851910793 1104 20-21 0.07994718115247679 1104 22-23 0.11148002426924819 1104 24-25 0.11552015731071918 1104 26-27 0.10271479866980116 1104 28-29 0.0925922635138221 1104 30-31 0.14843019464215246 1104 32-33 0.11611595516074402 1104 34-35 0.13272171656007004 1104 36-37 0.09630051095007985 1104 38-39 0.16292629910983436 1104 40-41 0.0862335019941014 1104 42-43 0.07689587067770987 1104 44-45 0.08829067897807619 1104 46-47 0.11769870567749763 1104 48-49 0.08196995139899599 1104 50-51 0.07259886054468723 1104 52-53 0.07896629458033289 1104 54-55 0.028093673541050634 1104 56-57 0.06274286410317842 1104 58-59 0.10884515206882384 1104 60-61 0.13685865048288548 1104 62-63 0.13902448133369205 1104 64-65 0.12340266388174825 1104 66-67 0.10596213947731314 1104 68-69 0.10619106401981782 1104 70-71 0.07085814638588772 1104 72-73 0.031420388450527525 1104 74-75 0.06981450049107707 1104 76-77 0.016699511071465167 1104 78-79 0.08717134188069764 1104 80-81 0.09904269975618263 1104 82-83 0.10496170557621554 1104 84-85 0.05408482604980236 1104 86-87 0.06657789239823586 1104 88-89 0.09234691270484774 1104 90-91 0.098577535721315 1104 92-93 0.13316457416919292 1104 94-95 0.1753082384216924 1104 96-97 0.21244330187733595 1104 98-99 0.17991099151288736 1104 100 0.2264534577840962 1105 1 0.15321310502979557 1105 2 0.20419075344953797 1105 3 0.15017141482546137 1105 4 0.12207046927035492 1105 5 0.15062039153543338 1105 6 0.19125911716066923 1105 7 0.14104259205138447 1105 8 0.19587537595582916 1105 9 0.18723148942457613 1105 10-11 0.14013016089078434 1105 12-13 0.24274927334055008 1105 14-15 0.26344893595007335 1105 16-17 0.22777629060698246 1105 18-19 0.21295544230403607 1105 20-21 0.20021888071880767 1105 22-23 0.21229028773670677 1105 24-25 0.20872731709255277 1105 26-27 0.25815723273890256 1105 28-29 0.2792473681145822 1105 30-31 0.26745094677950476 1105 32-33 0.30403491234879 1105 34-35 0.3258417492175383 1105 36-37 0.3138330486367309 1105 38-39 0.3487480939048666 1105 40-41 0.368479240337507 1105 42-43 0.27685861630403963 1105 44-45 0.25616694877025026 1105 46-47 0.31698871430121045 1105 48-49 0.28544260341545424 1105 50-51 0.24984067814278177 1105 52-53 0.32026617827074233 1105 54-55 0.33051949471580144 1105 56-57 0.318826088727441 1105 58-59 0.28635159957985934 1105 60-61 0.335861890172076 1105 62-63 0.27961876436833677 1105 64-65 0.3312338229760883 1105 66-67 0.3474224105518928 1105 68-69 0.28629508256251057 1105 70-71 0.27620586598754926 1105 72-73 0.27894415557403107 1105 74-75 0.31088463775202513 1105 76-77 0.26606602107807475 1105 78-79 0.29146180267940736 1105 80-81 0.24504361369849903 1105 82-83 0.2840248687116116 1105 84-85 0.2851628107347892 1105 86-87 0.22683820279151945 1105 88-89 0.2695174305394019 1105 90-91 0.31910559057130783 1105 92-93 0.34693046203840083 1105 94-95 0.3786193410952805 1105 96-97 0.3467619819555452 1105 98-99 0.36083291772791526 1105 100 0.3659610374879101 1106 1 0.1486211640522619 1106 2 0.16297448111082247 1106 3 0.12079916449279793 1106 4 0.12256408606520353 1106 5 0.14104938460074123 1106 6 0.11103502037349244 1106 7 0.10708465814926882 1106 8 0.12882555660488038 1106 9 0.18896300694152046 1106 10-11 0.13969881689408226 1106 12-13 0.14013861548209405 1106 14-15 0.10373616164152821 1106 16-17 0.19499967423504927 1106 18-19 0.17326675391795732 1106 20-21 0.14966018224485822 1106 22-23 0.1879334542670037 1106 24-25 0.12750245217376488 1106 26-27 0.21619811335924766 1106 28-29 0.20884670673898853 1106 30-31 0.21140258943718493 1106 32-33 0.2023366309389658 1106 34-35 0.20412929871827146 1106 36-37 0.1879361650669793 1106 38-39 0.15430868628766348 1106 40-41 0.17625830405127374 1106 42-43 0.13762871412539823 1106 44-45 0.18235507296988374 1106 46-47 0.14225782456452407 1106 48-49 0.18364579100401812 1106 50-51 0.12007427135785775 1106 52-53 0.08780899562840006 1106 54-55 0.14952920380287793 1106 56-57 0.11511996961271365 1106 58-59 0.1159976625021315 1106 60-61 0.11160045204726998 1106 62-63 0.1511236774997542 1106 64-65 0.22557819446997485 1106 66-67 0.22505917802494224 1106 68-69 0.2444101134685397 1106 70-71 0.25331832987303926 1106 72-73 0.2383797545155204 1106 74-75 0.20333077815490697 1106 76-77 0.23201989248546795 1106 78-79 0.23517850283942465 1106 80-81 0.23855622270023957 1106 82-83 0.19968763854263472 1106 84-85 0.20952162803460794 1106 86-87 0.18424262389599022 1106 88-89 0.23219406558159505 1106 90-91 0.16136958941289592 1106 92-93 0.17690073981355425 1106 94-95 0.24815679766191678 1106 96-97 0.26354735397097073 1106 98-99 0.3050745912499231 1106 100 0.3160630517800165 1107 1 0.03724767031419418 1107 2 0.09075251739364987 1107 3 0.06865317306012386 1107 4 0.07962627956046475 1107 5 0.10355463695337619 1107 6 0.05238985944704666 1107 7 0.09316416114275938 1107 8 0.026101876884176534 1107 9 0.13242195962945402 1107 10-11 0.17822640972325132 1107 12-13 0.14824010503519247 1107 14-15 0.14510407872062103 1107 16-17 0.21348354915996026 1107 18-19 0.140333974161436 1107 20-21 0.11745303791725803 1107 22-23 0.20159221490919066 1107 24-25 0.19458169290213334 1107 26-27 0.19182437709643096 1107 28-29 0.19822355916268464 1107 30-31 0.1917878724156168 1107 32-33 0.2231650291758669 1107 34-35 0.24106391292063023 1107 36-37 0.2528934905831193 1107 38-39 0.1976240276694341 1107 40-41 0.2043994576794148 1107 42-43 0.19238396154293014 1107 44-45 0.18607684069322517 1107 46-47 0.2126569751639309 1107 48-49 0.14952735432976993 1107 50-51 0.1003409357684859 1107 52-53 0.18034469752601723 1107 54-55 0.12026267193484586 1107 56-57 0.12887690342321179 1107 58-59 0.08928310424966668 1107 60-61 0.1399922380727716 1107 62-63 0.26204631492808517 1107 64-65 0.2704584901809497 1107 66-67 0.2173080803184959 1107 68-69 0.25447718142904563 1107 70-71 0.249900070580253 1107 72-73 0.2038487085288807 1107 74-75 0.22647567540620628 1107 76-77 0.2824669769714987 1107 78-79 0.21736562477578758 1107 80-81 0.1992621036431501 1107 82-83 0.23456431753294282 1107 84-85 0.25368104715965245 1107 86-87 0.2971930571974539 1107 88-89 0.31531184095292275 1107 90-91 0.36902293695930766 1107 92-93 0.36547641458223623 1107 94-95 0.4226926518425067 1107 96-97 0.40499285781611505 1107 98-99 0.33717104841469236 1107 100 0.32213075284289516 1108 1 0.05844099462140662 1108 2 0.06410004289740456 1108 3 0.12227223850942437 1108 4 0.0684893933077717 1108 5 0.07619286389915914 1108 6 0.05400970517641923 1108 7 0.04824472258071921 1108 8 0.03613027362353449 1108 9 0.15583997070876876 1108 10-11 0.10804462884969723 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-0.05328865324246834 2214 94-95 -0.05202310043869929 2214 96-97 -0.1545144999265986 2214 98-99 -0.07619651108555558 2214 100 -0.08987071803851521 2215 1 -0.22650851039486852 2215 2 -0.2604359790723443 2215 3 -0.24706396285039744 2215 4 -0.21595294717901936 2215 5 -0.15284258225059943 2215 6 -0.15702250752262614 2215 7 -0.20497128742968584 2215 8 -0.26292211492207684 2215 9 -0.3308472372286886 2215 10-11 -0.2505568206663895 2215 12-13 -0.28475935061809565 2215 14-15 -0.3319559521075277 2215 16-17 -0.3205951225638728 2215 18-19 -0.3623023524506479 2215 20-21 -0.30212036903266437 2215 22-23 -0.2652835998673524 2215 24-25 -0.3908944294169103 2215 26-27 -0.3104257062921576 2215 28-29 -0.2811866952651343 2215 30-31 -0.2874268125491568 2215 32-33 -0.31813973653196825 2215 34-35 -0.30115529613519243 2215 36-37 -0.29178809061058786 2215 38-39 -0.30049004608071783 2215 40-41 -0.3650355902490503 2215 42-43 -0.2859079758537746 2215 44-45 -0.2984529977365824 2215 46-47 -0.2643384601014418 2215 48-49 -0.26618942133094237 2215 50-51 -0.3337040664535209 2215 52-53 -0.3215700948948097 2215 54-55 -0.29146725598471335 2215 56-57 -0.26821514160506155 2215 58-59 -0.22483598196097887 2215 60-61 -0.1746327497226119 2215 62-63 -0.26770523001248137 2215 64-65 -0.21480308951817761 2215 66-67 -0.22811609582482362 2215 68-69 -0.2948669375989752 2215 70-71 -0.3621209853747658 2215 72-73 -0.2910139813308561 2215 74-75 -0.25057701186804593 2215 76-77 -0.24324148430824977 2215 78-79 -0.23499904114654413 2215 80-81 -0.16049924840581298 2215 82-83 -0.21599169110602645 2215 84-85 -0.19249707642099878 2215 86-87 -0.1999361212731614 2215 88-89 -0.25635638633441005 2215 90-91 -0.28209368505023846 2215 92-93 -0.2695233789669089 2215 94-95 -0.27008282449319054 2215 96-97 -0.20749024161809615 2215 98-99 -0.22174546239774173 2215 100 -0.22551972096412598 2216 1 -0.4620837482110467 2216 2 -0.4999956871853932 2216 3 -0.608268298892991 2216 4 -0.5595916913488708 2216 5 -0.4339684912320223 2216 6 -0.4780236636234463 2216 7 -0.36451571717613973 2216 8 -0.541315140286386 2216 9 -0.5830168352766307 2216 10-11 -0.5784554735388028 2216 12-13 -0.5643514226337203 2216 14-15 -0.597331853165862 2216 16-17 -0.6216899542089038 2216 18-19 -0.7042163115285618 2216 20-21 -0.6970187330568081 2216 22-23 -0.7349103753542394 2216 24-25 -0.7802769633687703 2216 26-27 -0.8689890035001966 2216 28-29 -0.675836553841151 2216 30-31 -0.725187042382089 2216 32-33 -0.8002545792354923 2216 34-35 -0.7862253955114227 2216 36-37 -0.7816182253406438 2216 38-39 -0.7399414813137142 2216 40-41 -0.8349909984628923 2216 42-43 -0.7847886711405607 2216 44-45 -0.8348580864675696 2216 46-47 -0.7645918570175709 2216 48-49 -0.8330539722568275 2216 50-51 -0.8124874812942835 2216 52-53 -0.884246369599964 2216 54-55 -0.9220712209520485 2216 56-57 -0.8480887561380399 2216 58-59 -0.8197460713529523 2216 60-61 -0.7625893892966857 2216 62-63 -0.8359569289128927 2216 64-65 -0.8392784461529885 2216 66-67 -0.8715149378680778 2216 68-69 -0.904622404305961 2216 70-71 -0.9461887977931056 2216 72-73 -0.8503320666529959 2216 74-75 -0.8794810737446426 2216 76-77 -0.8176937322956093 2216 78-79 -0.7618970349290279 2216 80-81 -0.7687830988781954 2216 82-83 -0.671573441966899 2216 84-85 -0.6269451926120801 2216 86-87 -0.7049894323762729 2216 88-89 -0.7752251376314945 2216 90-91 -0.7463170398596297 2216 92-93 -0.7952780668137898 2216 94-95 -0.8325781935233891 2216 96-97 -0.9087580204724688 2216 98-99 -0.9704375727193799 2216 100 -0.9700646135126512 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 63076.0 3 13223.0 4 9427.0 5 8297.0 6 5879.0 7 5513.0 8 6203.0 9 6635.0 10 6894.0 11 7651.0 12 8627.0 13 9752.0 14 10581.0 15 11108.0 16 10995.0 17 10760.0 18 10698.0 19 10067.0 20 10005.0 21 10722.0 22 12691.0 23 19063.0 24 30415.0 25 39226.0 26 37976.0 27 31175.0 28 33520.0 29 41351.0 30 48798.0 31 58151.0 32 75089.0 33 100752.0 34 143886.0 35 225974.0 36 400585.0 37 818719.0 38 1402138.0 39 737303.0 40 3269.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.09833754320615 21.945844213873254 14.281310544491465 22.67450769842913 2 14.820128780173608 22.528968421923544 40.26058513109658 22.39031766680627 3 16.891319786014428 28.67066812775525 31.151946169546623 23.2860659166837 4 11.735246953531767 19.517813971384662 40.52798035670201 28.218958718381565 5 12.055495875396725 39.80774833803732 35.92953144773686 12.207224338829098 6 27.39995814236622 40.93623205231626 19.139036068958784 12.524773736358737 7 24.13125948997296 34.73098241111981 24.614468284613228 16.523289814294003 8 23.28918636517908 37.10749580645319 22.110478329004486 17.49283949936324 9 24.398013075058593 18.082137914867552 22.365805390069912 35.15404362000394 10-11 22.06143685081204 28.712117404186742 30.372543978306986 18.853901766694232 12-13 22.984350764224143 26.979907895433335 29.54041796239219 20.495323377950328 14-15 20.856729046833834 28.47977645092716 27.051223768369425 23.61227073386958 16-17 20.269654734648906 31.467625729672697 27.465429650055135 20.797289885623265 18-19 20.258934556649468 30.521714587938153 29.26054792119735 19.95880293421503 20-21 21.819532253279107 29.544488071466667 28.546010247778455 20.089969427475772 22-23 21.340916339464002 29.635364933096746 28.52094460336898 20.50277412407027 24-25 21.072856286895096 29.82276787878815 28.346886277593892 20.757489556722863 26-27 20.893048654039394 29.71063970994134 28.49166428316928 20.904647352849988 28-29 20.915589941181363 29.544732722831803 28.719067727059823 20.82060960892702 30-31 21.17778948150369 29.890102606782538 28.30879850824942 20.62330940346435 32-33 20.677844416855677 29.722738831998797 28.79304140346257 20.806375347682952 34-35 21.057587817607516 29.43542916520061 28.655803108139903 20.851179909051968 36-37 21.32759396058088 29.65395843684681 28.205188655115858 20.813258947456447 38-39 21.077371216633445 29.675432154395477 28.407037151866664 20.840159477104415 40-41 20.912709727382758 29.50671167658691 28.64880830320044 20.931770292829892 42-43 20.881705727110532 29.466599973221797 28.649130798181748 21.002563501485923 44-45 21.003397540230694 29.50033962057687 28.530808501590453 20.96545433760198 46-47 21.122120175419475 29.563259503482282 28.399897780211443 20.914722540886803 48-49 20.94719444934983 29.382050685535365 28.575112639712607 21.095642225402194 50-51 20.991921167102664 29.42833427561177 28.34893245264773 21.230812104637835 52-53 20.911375265391126 29.43378332874427 28.50771118861864 21.147130217245962 54-55 21.117160425017246 29.65211243109172 28.329082330522215 20.90164481336882 56-57 20.987083742383003 29.4586265628218 28.367448112781613 21.18684158201359 58-59 20.99921622599025 29.14033513678458 28.49791401349675 21.362534623728425 60-61 21.237117437548292 29.431548104908284 28.263538005699935 21.067796451843492 62-63 20.938158126219225 29.469308813033606 28.456274007810606 21.136259052936563 64-65 21.047441807707298 29.281219609703097 28.489082707201707 21.1822558753879 66-67 20.951520330306032 29.301527024857023 28.333597260260568 21.413355384576377 68-69 21.296033934478807 29.410363520791137 28.250337952499383 21.043264592230674 70-71 21.285758577143245 29.293798265822158 28.338334600330857 21.082108556703734 72-73 21.06273661679189 29.293486891357446 28.523791455617797 21.119985036232865 74-75 21.08665684799188 29.286825701915888 28.43994276047697 21.186574689615263 76-77 21.14095833053467 29.441856823793632 28.219100421378613 21.19808442429308 78-79 21.202121171817765 29.487373098224857 28.301192074897124 21.009313655060257 80-81 21.126279248626727 29.40390250064833 28.36370049868845 21.1061177520365 82-83 20.990743494101338 29.417646811555297 28.545930787162195 21.04567890718117 84-85 21.090469606040514 29.251977181483007 28.344716717973263 21.312836494503216 86-87 21.188851121988325 29.39225192234018 28.293244383107986 21.12565257256351 88-89 21.022591295327384 29.317839498868793 28.379252715928672 21.280316489875155 90-91 20.992773042668475 29.255443015620486 28.378279493505794 21.37350444820525 92-93 21.00231855698547 29.386644982763453 28.44568828358613 21.165348176664946 94-95 21.024722112961264 29.178836891793175 28.419659499481387 21.376781495764178 96-97 20.96517664119285 29.403063085504836 28.29227200341628 21.339488269886033 98-99 20.862041585429488 29.323330428589795 28.352054027340174 21.462573958640544 100 20.98268757792903 29.294376120698974 28.25309080491931 21.469845496452685 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13011.0 1 8910.5 2 3975.0 3 2640.0 4 1807.5 5 1306.0 6 1197.0 7 1356.5 8 1670.5 9 1741.5 10 1321.5 11 987.0 12 927.5 13 943.5 14 1010.0 15 1118.5 16 1374.0 17 1785.5 18 2359.0 19 3292.5 20 4686.0 21 6506.5 22 9297.0 23 13655.0 24 19557.0 25 26860.5 26 36272.0 27 47801.0 28 62347.5 29 79637.5 30 95374.5 31 110085.5 32 124543.0 33 136380.0 34 145658.5 35 154673.0 36 161870.0 37 167338.0 38 172993.0 39 175072.0 40 176251.5 41 175041.5 42 169035.0 43 164913.0 44 159766.5 45 152270.0 46 146965.5 47 141245.0 48 132623.0 49 127104.5 50 123268.0 51 116646.5 52 109000.0 53 101160.5 54 94583.0 55 88652.5 56 80013.0 57 69804.5 58 62422.5 59 56719.5 60 49606.0 61 41541.0 62 34676.0 63 28280.5 64 22541.0 65 17855.0 66 13957.0 67 10776.0 68 8146.5 69 6067.0 70 4494.5 71 3430.5 72 2619.5 73 1971.0 74 1475.5 75 1074.0 76 764.5 77 558.5 78 415.0 79 299.5 80 232.0 81 167.5 82 128.0 83 112.0 84 94.0 85 81.0 86 68.5 87 57.5 88 51.5 89 42.0 90 32.5 91 26.0 92 24.5 93 22.0 94 17.0 95 16.0 96 13.0 97 14.5 98 18.0 99 18.0 100 48.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.780975642954908E-4 2 1.11205165969262E-4 3 6.672309958155721E-5 4 8.89641327754096E-5 5 8.89641327754096E-5 6 2.22410331938524E-5 7 2.22410331938524E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 2.22410331938524E-5 64-65 3.3361549790778606E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.11205165969262E-4 84-85 4.670616970709004E-4 86-87 0.0010342080435141367 88-89 0.0028023701824254027 90-91 0.0024687546845176167 92-93 0.0017459211057174134 94-95 0.001723680072523561 96-97 0.0016458364563450775 98-99 0.00222410331938524 100 0.0025799598504868786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4496194.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.31632228645778 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6790176043212 47.16038051353393 2 14.98851773697695 18.68058603787489 3 4.698690525270302 8.784153392912092 4 1.908352705558956 4.756860889433823 5 0.8810502056470848 2.7451904282826822 6 0.4848781126682391 1.8129492443210025 7 0.3131004581031794 1.365788833863671 8 0.20078083107435796 1.0009538382538021 9 0.12281428552546414 0.6888001138367285 >10 0.6507990559762191 7.193925824274289 >50 0.04585405509336305 1.9799759955774816 >100 0.02464435842045755 2.801414369635947 >500 0.0012500546334825657 0.507276972155526 >1k 2.1429491194553113E-4 0.17135498511203062 >5k 0.0 0.0 >10k+ 3.5715818657568886E-5 0.3503885609322515 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15743 0.35014058557081834 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 1.11205165969262E-5 0.0 0.0 0.0 0.0 16-17 2.22410331938524E-5 0.0 0.0 0.0 0.0 18-19 2.22410331938524E-5 0.0 0.0 2.22410331938524E-5 0.0 20-21 2.22410331938524E-5 0.0 0.0 4.44820663877048E-5 0.0 22-23 2.22410331938524E-5 0.0 0.0 1.66807748953893E-4 0.0 24-25 2.22410331938524E-5 0.0 0.0 2.668923983262288E-4 0.0 26-27 2.22410331938524E-5 0.0 0.0 4.893027302647528E-4 0.0 28-29 2.22410331938524E-5 0.0 0.0 0.0014790287073911845 0.0 30-31 2.22410331938524E-5 0.0 0.0 0.004270278373219661 0.0 32-33 2.22410331938524E-5 0.0 0.0 0.009875018738070465 0.0 34-35 2.22410331938524E-5 0.0 0.0 0.01914952957990692 0.0 36-37 2.22410331938524E-5 0.0 0.0 0.03396205768701262 2.22410331938524E-5 38-39 2.22410331938524E-5 0.0 0.0 0.05350080534781195 2.22410331938524E-5 40-41 2.22410331938524E-5 0.0 0.0 0.07325084282395289 2.22410331938524E-5 42-43 2.22410331938524E-5 0.0 0.0 0.0924003724038598 2.22410331938524E-5 44-45 2.22410331938524E-5 0.0 0.0 0.11880047880496258 2.22410331938524E-5 46-47 2.22410331938524E-5 0.0 0.0 0.15208418497956272 2.22410331938524E-5 48-49 2.22410331938524E-5 0.0 0.0 0.1863687376478862 2.22410331938524E-5 50-51 2.22410331938524E-5 0.0 0.0 0.220753374965582 2.22410331938524E-5 52-53 2.22410331938524E-5 0.0 0.0 0.2548711198849516 2.22410331938524E-5 54-55 2.22410331938524E-5 0.0 0.0 0.2949383411836767 2.22410331938524E-5 56-57 2.22410331938524E-5 0.0 0.0 0.34317914218114254 2.22410331938524E-5 58-59 4.44820663877048E-5 0.0 0.0 0.39860379690022274 2.22410331938524E-5 60-61 4.44820663877048E-5 0.0 0.0 0.4602225793637908 2.22410331938524E-5 62-63 4.44820663877048E-5 0.0 0.0 0.5226309185057406 2.22410331938524E-5 64-65 4.44820663877048E-5 0.0 0.0 0.5921341472365294 2.22410331938524E-5 66-67 4.44820663877048E-5 0.0 0.0 0.6722797103505764 2.22410331938524E-5 68-69 6.67230995815572E-5 0.0 0.0 0.7670265117563877 2.22410331938524E-5 70-71 6.67230995815572E-5 0.0 0.0 0.8722043577301157 2.22410331938524E-5 72-73 6.67230995815572E-5 0.0 0.0 0.9807294791995185 2.22410331938524E-5 74-75 6.67230995815572E-5 0.0 0.0 1.09461468966864 2.22410331938524E-5 76-77 6.67230995815572E-5 0.0 0.0 1.22232270226774 2.22410331938524E-5 78-79 8.89641327754096E-5 0.0 0.0 1.3738508614174565 2.22410331938524E-5 80-81 8.89641327754096E-5 0.0 0.0 1.5415371311825068 2.22410331938524E-5 82-83 8.89641327754096E-5 0.0 0.0 1.721266920422028 2.22410331938524E-5 84-85 1.0008464937233581E-4 0.0 0.0 1.9071018732732616 2.22410331938524E-5 86-87 1.11205165969262E-4 0.0 0.0 2.10782719784778 2.22410331938524E-5 88 1.11205165969262E-4 0.0 0.0 2.273678582374337 2.22410331938524E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4140 0.0 46.3185 1 GTATCAA 7455 0.0 38.961487 1 TCAACGC 8245 0.0 33.632256 4 ATCAACG 8260 0.0 33.57118 3 CAACGCA 8950 0.0 31.088037 5 AACGCAG 9290 0.0 29.950266 6 TATCAAC 9910 0.0 28.408468 2 ACGCAGA 10390 0.0 26.28181 7 CGCAGAG 10480 0.0 25.966413 8 GTACATG 16510 0.0 24.538887 1 TACATGG 16130 0.0 23.718363 2 ACATGGG 16535 0.0 22.767902 3 GCAGAGT 12495 0.0 21.327574 9 CATGGGT 4620 0.0 21.261747 4 CATGGGG 9600 0.0 18.652988 4 AGAGTAC 10995 0.0 17.611513 10-11 CAGAGTA 12210 0.0 17.552698 10-11 GAGTACA 11925 0.0 16.395685 1 GTATAGA 1865 0.0 15.624551 1 GGACCGT 285 1.7503373E-5 14.841996 6 >>END_MODULE