FastQCFastQC Report
Wed 25 May 2016
SRR2431378_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431378_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496194
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197070.43830404115124927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149220.3318806973186655No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97600.21707248397199944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76400.060.2941471
GTATCAA156750.046.0302431
ATCAACG198250.036.0346033
TCAACGC199400.035.897494
CAACGCA207850.034.460335
TATCAAC211000.034.124452
AACGCAG215350.033.303836
ACGCAGA244400.029.3644837
CGCAGAG245300.029.1417878
GTGGTAT29100.026.490691
GCAGAGT272000.025.9010439
TGGTATC30150.024.6296982
GTACATG158050.024.0005661
TACATGG156800.023.0799032
ACATGGG157900.022.3833473
AGAGTAC255950.021.86043410-11
GAGTACT182650.021.70450812-13
CAGAGTA268650.021.14191410-11
AGTACTT191150.020.55495312-13
CATGGGT46150.019.7569264
Produced by FastQC (version 0.11.5)