##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2431378_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4496194
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.770918247744646	34.0	31.0	34.0	31.0	34.0
2	32.912661241930394	34.0	31.0	34.0	31.0	34.0
3	32.98362014628373	34.0	31.0	34.0	31.0	34.0
4	36.36181779522859	37.0	37.0	37.0	35.0	37.0
5	36.31081065452247	37.0	37.0	37.0	35.0	37.0
6	36.33789422787362	37.0	37.0	37.0	35.0	37.0
7	36.2058463224674	37.0	37.0	37.0	35.0	37.0
8	36.26138151512146	37.0	37.0	37.0	35.0	37.0
9	38.086328347931605	39.0	39.0	39.0	35.0	39.0
10-11	38.080623522917385	39.0	39.0	39.0	36.0	39.0
12-13	38.06094743687662	39.0	39.0	39.0	35.0	39.0
14-15	39.56980982137337	41.0	40.0	41.0	37.0	41.0
16-17	39.57658466249454	41.0	40.0	41.0	37.0	41.0
18-19	39.64029677100232	41.0	40.0	41.0	37.0	41.0
20-21	39.64819033609315	41.0	40.0	41.0	37.0	41.0
22-23	39.582842288388804	41.0	40.0	41.0	37.0	41.0
24-25	39.5594877578681	41.0	40.0	41.0	37.0	41.0
26-27	39.48017512144716	41.0	40.0	41.0	36.0	41.0
28-29	39.404018042815764	41.0	39.5	41.0	36.0	41.0
30-31	39.26539791210077	41.0	39.0	41.0	36.0	41.0
32-33	39.096361834031185	41.0	39.0	41.0	35.0	41.0
34-35	39.009973101694456	41.0	39.0	41.0	35.0	41.0
36-37	38.910705921497154	41.0	39.0	41.0	35.0	41.0
38-39	38.803938842496564	41.0	39.0	41.0	35.0	41.0
40-41	38.72238152980054	41.0	39.0	41.0	35.0	41.0
42-43	38.61490218171191	41.0	39.0	41.0	35.0	41.0
44-45	38.44915288352772	41.0	38.0	41.0	35.0	41.0
46-47	38.204753731711754	41.0	38.0	41.0	34.0	41.0
48-49	37.99095368215873	41.0	38.0	41.0	34.0	41.0
50-51	37.86560688884866	40.0	38.0	41.0	33.0	41.0
52-53	37.769557652539014	40.0	38.0	41.0	33.0	41.0
54-55	37.639980392305134	40.0	37.5	41.0	33.0	41.0
56-57	37.49344968210892	40.0	37.0	41.0	33.0	41.0
58-59	37.32817956698487	40.0	37.0	41.0	33.0	41.0
60-61	37.14861458380132	40.0	36.5	41.0	33.0	41.0
62-63	36.93899996307988	39.5	36.0	41.0	32.0	41.0
64-65	36.6822254555742	39.0	35.5	41.0	32.0	41.0
66-67	36.40793758009552	39.0	35.0	41.0	31.5	41.0
68-69	36.14805088481502	38.5	35.0	41.0	31.0	41.0
70-71	35.89380851893846	37.5	35.0	40.0	31.0	41.0
72-73	35.626979841172336	37.0	35.0	40.0	31.0	41.0
74-75	35.31180772004055	37.0	35.0	39.0	31.0	41.0
76-77	34.96877870483347	36.0	35.0	39.0	31.0	41.0
78-79	34.63064482982718	36.0	35.0	38.5	31.0	40.0
80-81	34.295578215708666	35.0	35.0	37.0	31.0	39.0
82-83	33.9506270192078	35.0	35.0	37.0	30.5	39.0
84-85	33.6624645199918	35.0	35.0	36.5	30.0	38.5
86-87	33.41526711258456	35.0	34.0	36.0	30.0	37.0
88-89	33.17448235107293	35.0	34.0	36.0	30.0	37.0
90-91	32.96648131730971	35.0	34.0	35.5	30.0	36.5
92-93	32.81098491301754	35.0	34.0	35.0	30.0	36.0
94-95	32.66963947730014	35.0	34.0	35.0	29.5	36.0
96-97	32.53027905379528	35.0	34.0	35.0	29.0	36.0
98-99	32.40701446601281	35.0	34.0	35.0	29.0	36.0
100	32.30363525239347	35.0	34.0	35.0	29.0	36.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1812504387179814
1101	2	0.16237118642860793
1101	3	0.17815565512266573
1101	4	0.10612116291955687
1101	5	0.14242348946490324
1101	6	0.13245100843342072
1101	7	0.14951982228802052
1101	8	0.16924687482436696
1101	9	0.19048386743346413
1101	10-11	0.1927591267825477
1101	12-13	0.203206919146119
1101	14-15	0.26529280347720885
1101	16-17	0.28348856261565203
1101	18-19	0.2549744068022406
1101	20-21	0.30107489505390816
1101	22-23	0.28041858593937263
1101	24-25	0.28189528028244126
1101	26-27	0.25601294364737015
1101	28-29	0.24527445479377263
1101	30-31	0.28299881727319587
1101	32-33	0.2788855790319005
1101	34-35	0.27261687565400905
1101	36-37	0.2630758397669837
1101	38-39	0.24324021850614486
1101	40-41	0.21756380983006807
1101	42-43	0.2271332181270509
1101	44-45	0.2205209293263266
1101	46-47	0.23978622464697708
1101	48-49	0.2386265212576859
1101	50-51	0.20360269339774817
1101	52-53	0.12168465193092004
1101	54-55	0.13111709474901545
1101	56-57	0.17645631960050423
1101	58-59	0.19585448774329706
1101	60-61	0.1615597148331318
1101	62-63	0.149583708056916
1101	64-65	0.20676628824384835
1101	66-67	0.23901032386015686
1101	68-69	0.25503285758428973
1101	70-71	0.24593118018103866
1101	72-73	0.2493880544267384
1101	74-75	0.22545452311113934
1101	76-77	0.18874710314225496
1101	78-79	0.17667259579121009
1101	80-81	0.17941664776856925
1101	82-83	0.2681920459442395
1101	84-85	0.19368164664604848
1101	86-87	0.2007986555258512
1101	88-89	0.19088506162280794
1101	90-91	0.20754375649247692
1101	92-93	0.2449843826378313
1101	94-95	0.23886376560991351
1101	96-97	0.24444702061769163
1101	98-99	0.3087157256417683
1101	100	0.330818850723233
1102	1	0.08697884828921332
1102	2	0.13347034269643387
1102	3	0.1348900483820259
1102	4	0.10622486320027491
1102	5	0.1395397423310598
1102	6	0.08493323535753206
1102	7	0.13018760358109915
1102	8	0.16606218462440836
1102	9	0.16232135757132227
1102	10-11	0.2014487251592385
1102	12-13	0.19625202634249206
1102	14-15	0.2488297016744596
1102	16-17	0.27169796198484164
1102	18-19	0.26337769234248043
1102	20-21	0.26514812989016434
1102	22-23	0.24914158065793401
1102	24-25	0.23988355244777182
1102	26-27	0.2543642759534066
1102	28-29	0.24537936794326498
1102	30-31	0.22939145746415335
1102	32-33	0.28669850069331204
1102	34-35	0.24182342599442563
1102	36-37	0.24040906576143328
1102	38-39	0.2280121961432755
1102	40-41	0.20626707420575485
1102	42-43	0.27655936442450013
1102	44-45	0.3241076552052391
1102	46-47	0.22371215050551996
1102	48-49	0.2374489014762844
1102	50-51	0.24156154439863542
1102	52-53	0.20011382615528106
1102	54-55	0.18301142901443512
1102	56-57	0.22541391952724155
1102	58-59	0.20041760331209701
1102	60-61	0.1564414085801289
1102	62-63	0.14112743531183725
1102	64-65	0.15784173899137244
1102	66-67	0.22911066146217962
1102	68-69	0.20139987592225594
1102	70-71	0.20500027003824073
1102	72-73	0.1900405657018709
1102	74-75	0.14148094667038436
1102	76-77	0.167388862942083
1102	78-79	0.17177912508835647
1102	80-81	0.13284567038432726
1102	82-83	0.15527578179840162
1102	84-85	0.1630564825297398
1102	86-87	0.1545020138988633
1102	88-89	0.17774203649762654
1102	90-91	0.2127374880227606
1102	92-93	0.21102890826362142
1102	94-95	0.21339769045935242
1102	96-97	0.24908184705314085
1102	98-99	0.2684941921268589
1102	100	0.302820532972234
1103	1	0.11813812248276179
1103	2	0.16152556881914393
1103	3	0.13503335167108332
1103	4	0.11245905042355275
1103	5	0.11288365045882642
1103	6	0.14248571401028443
1103	7	0.08183401012758651
1103	8	0.10320076384642363
1103	9	0.20312553609757344
1103	10-11	0.1618247727556934
1103	12-13	0.1820716223260419
1103	14-15	0.22299167392303332
1103	16-17	0.28435964762684307
1103	18-19	0.21796497886999333
1103	20-21	0.22614468072823968
1103	22-23	0.27261792079076486
1103	24-25	0.2584651957905919
1103	26-27	0.2216627618800331
1103	28-29	0.2560064927851329
1103	30-31	0.30625914405491983
1103	32-33	0.23239189095264834
1103	34-35	0.21402841887867652
1103	36-37	0.19392587671968897
1103	38-39	0.18180582952404478
1103	40-41	0.17844790319057324
1103	42-43	0.16824134971861326
1103	44-45	0.129952057482285
1103	46-47	0.1349622493353806
1103	48-49	0.1129411235667277
1103	50-51	0.13738430293753368
1103	52-53	0.14972448594338772
1103	54-55	0.18604021815106364
1103	56-57	0.17471227697505753
1103	58-59	0.11932919012424748
1103	60-61	0.09045819977240654
1103	62-63	0.10019497563441604
1103	64-65	0.1083485879002879
1103	66-67	0.13137070257540273
1103	68-69	0.164701781361849
1103	70-71	0.13759717271059912
1103	72-73	0.11140150221356748
1103	74-75	0.12191772521244815
1103	76-77	0.13868585653791143
1103	78-79	0.10336914920284102
1103	80-81	0.08723598901178065
1103	82-83	0.1233442511216154
1103	84-85	0.15726203874416456
1103	86-87	0.11550330856995572
1103	88-89	0.12462904432494781
1103	90-91	0.1824419867577376
1103	92-93	0.17848275472155706
1103	94-95	0.16147801936511286
1103	96-97	0.15244917785642542
1103	98-99	0.1625361355171222
1103	100	0.17134273015756207
1104	1	2.983231902362604E-4
1104	2	0.04666408389142873
1104	3	0.07342971048375802
1104	4	-0.04427541589450357
1104	5	-0.008573833776793549
1104	6	-0.008499992310845528
1104	7	9.997956551188736E-4
1104	8	0.04578799662095179
1104	9	0.04043602710034122
1104	10-11	0.08434385342318507
1104	12-13	0.10441729481143369
1104	14-15	0.11065626270341511
1104	16-17	0.1655002582081977
1104	18-19	0.111535418734654
1104	20-21	0.09906010578976066
1104	22-23	0.11838878936966069
1104	24-25	0.08956180548760528
1104	26-27	0.1276328960695139
1104	28-29	0.09831008687157805
1104	30-31	0.1447589325815244
1104	32-33	0.11445779375178233
1104	34-35	0.07406720502140729
1104	36-37	0.13855447657358155
1104	38-39	0.13257335778457247
1104	40-41	0.10769902303830747
1104	42-43	0.1417569506875509
1104	44-45	0.13453833862654108
1104	46-47	0.1371297074251956
1104	48-49	0.15206776248552956
1104	50-51	0.08394867736079448
1104	52-53	0.10445593876558945
1104	54-55	0.10272924172072351
1104	56-57	0.040757556312186694
1104	58-59	0.0031262429541456527
1104	60-61	0.08845206223780622
1104	62-63	0.11149635405643465
1104	64-65	0.14863890722511286
1104	66-67	0.19501515812753922
1104	68-69	0.14488797407178566
1104	70-71	0.16863168612954382
1104	72-73	0.1620641108339882
1104	74-75	0.14353816411586706
1104	76-77	0.0785852865209904
1104	78-79	0.09119330427051153
1104	80-81	0.10763774039596541
1104	82-83	0.13498566914683607
1104	84-85	0.10536769373493371
1104	86-87	0.08699378746106845
1104	88-89	0.07694244308687104
1104	90-91	0.14288117854488291
1104	92-93	0.13891632323893788
1104	94-95	0.09623963117685719
1104	96-97	0.024139042513461106
1104	98-99	0.004995474951144274
1104	100	-0.047095106277382115
1105	1	-0.02781314138156432
1105	2	0.01934500098439429
1105	3	0.09030958338624373
1105	4	-0.04890668659621866
1105	5	-0.07877070073069348
1105	6	-0.07721617776756773
1105	7	-0.37087905348759165
1105	8	-0.3161516061503633
1105	9	-0.3225032062669939
1105	10-11	-0.2308580003113292
1105	12-13	-0.11718183873326637
1105	14-15	-0.05749178181157788
1105	16-17	-0.05487329321868373
1105	18-19	-0.07032106838928343
1105	20-21	-0.0848419849002724
1105	22-23	-0.11249558085687994
1105	24-25	-0.06824515272653287
1105	26-27	-0.07983199880956704
1105	28-29	-0.1298943245070987
1105	30-31	-0.1062875224777926
1105	32-33	-0.12613828908271785
1105	34-35	-0.20084959008290326
1105	36-37	-0.15125939544314804
1105	38-39	-0.17516013002828146
1105	40-41	-0.1988476104929191
1105	42-43	-0.20042915642991233
1105	44-45	-0.21249931689482793
1105	46-47	-0.21634564429105296
1105	48-49	-0.19575373306805233
1105	50-51	-0.2592112253135497
1105	52-53	-0.2384883340413424
1105	54-55	-0.2242838480159577
1105	56-57	-0.1640922887097176
1105	58-59	-0.1293726614671371
1105	60-61	-0.18802083042771756
1105	62-63	-0.2173682426986474
1105	64-65	-0.14645993615130948
1105	66-67	-0.14457337795827385
1105	68-69	-0.17764756962673545
1105	70-71	-0.18606031051155014
1105	72-73	-0.14367551765864306
1105	74-75	-0.15133545705326412
1105	76-77	-0.15509055350469225
1105	78-79	-0.16587314755410887
1105	80-81	-0.15385324001088208
1105	82-83	-0.1493878257149035
1105	84-85	-0.18015983625583942
1105	86-87	-0.22189510758098407
1105	88-89	-0.2362037573749376
1105	90-91	-0.27375923692012094
1105	92-93	-0.22746798169541904
1105	94-95	-0.24298107081332176
1105	96-97	-0.28460656077080415
1105	98-99	-0.27340749291882105
1105	100	-0.2619893054878375
1106	1	0.11825093966846367
1106	2	0.0950034379079554
1106	3	0.09910666481987107
1106	4	0.07829670901639929
1106	5	0.11335564440346246
1106	6	0.12746030311063095
1106	7	0.1487722670480025
1106	8	0.10662474741043582
1106	9	0.1368209296961922
1106	10-11	0.14328328630817566
1106	12-13	0.15644103553250233
1106	14-15	0.17483288283307274
1106	16-17	0.2139999302104627
1106	18-19	0.18518816226099943
1106	20-21	0.16181861022651134
1106	22-23	0.19141099316676957
1106	24-25	0.17790444391631155
1106	26-27	0.1609665753171754
1106	28-29	0.18251195342527637
1106	30-31	0.17043770518525037
1106	32-33	0.2032814526596809
1106	34-35	0.18744375192366647
1106	36-37	0.16434263349592726
1106	38-39	0.1525457192299271
1106	40-41	0.17742038372690416
1106	42-43	0.21730330906117956
1106	44-45	0.24821330315701573
1106	46-47	0.30974760833191795
1106	48-49	0.26847682497965053
1106	50-51	0.2899486411728276
1106	52-53	0.2684017675177799
1106	54-55	0.29629369638058733
1106	56-57	0.30350345705002724
1106	58-59	0.22688307564904875
1106	60-61	0.28379937263861876
1106	62-63	0.3155563150231089
1106	64-65	0.2985591739729543
1106	66-67	0.2742491627830148
1106	68-69	0.255691184476035
1106	70-71	0.25343852579594994
1106	72-73	0.27017646256535244
1106	74-75	0.25725140096216137
1106	76-77	0.27700396135336547
1106	78-79	0.22492633273802198
1106	80-81	0.20947485146852785
1106	82-83	0.25015941836498
1106	84-85	0.23751605763112593
1106	86-87	0.22982199008668402
1106	88-89	0.22325027774478912
1106	90-91	0.26912631490076677
1106	92-93	0.26214138361429207
1106	94-95	0.27043995708137203
1106	96-97	0.3046218998864205
1106	98-99	0.27039405074203415
1106	100	0.27684572179538947
1107	1	0.02775748642638831
1107	2	0.048643494534665876
1107	3	0.05457235461945231
1107	4	-0.04360757549331851
1107	5	-0.018572173466097297
1107	6	-0.031180486730406187
1107	7	-0.032046846157470554
1107	8	0.0027212271356447104
1107	9	0.05627909840880818
1107	10-11	0.07495810998423735
1107	12-13	0.10323153081878189
1107	14-15	0.06482059444294919
1107	16-17	0.08358507190413178
1107	18-19	0.10215058220668283
1107	20-21	0.06847178997964676
1107	22-23	0.07807826614529034
1107	24-25	0.08257406741075357
1107	26-27	0.05480877349371838
1107	28-29	0.059726930455923366
1107	30-31	0.1008101921636495
1107	32-33	0.10284906771088487
1107	34-35	0.06726022978763524
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2216	40-41	-0.6516104011898634
2216	42-43	-0.6510555348473162
2216	44-45	-0.6404691498153738
2216	46-47	-0.5961555685598086
2216	48-49	-0.6006932720956897
2216	50-51	-0.5496785529152817
2216	52-53	-0.6395978918422145
2216	54-55	-0.6466912679362409
2216	56-57	-0.6629502195812051
2216	58-59	-0.7258309245139358
2216	60-61	-0.666715075680635
2216	62-63	-0.6983139436443864
2216	64-65	-0.6186933472503142
2216	66-67	-0.5770441835516991
2216	68-69	-0.64180072108703
2216	70-71	-0.6151859311678152
2216	72-73	-0.6985853254924379
2216	74-75	-0.5579603378002886
2216	76-77	-0.5163024060585215
2216	78-79	-0.49937959383315444
2216	80-81	-0.5711610088148404
2216	82-83	-0.6093897699387512
2216	84-85	-0.6077954429657666
2216	86-87	-0.5347779012531149
2216	88-89	-0.4426983192265368
2216	90-91	-0.483477376227448
2216	92-93	-0.6427317173918183
2216	94-95	-0.587668546081197
2216	96-97	-0.7078656562319736
2216	98-99	-0.7014232054281457
2216	100	-0.7603428069123552
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	48.0
3	296.0
4	208.0
5	309.0
6	369.0
7	639.0
8	1143.0
9	1920.0
10	2735.0
11	3859.0
12	5811.0
13	8263.0
14	9680.0
15	12602.0
16	15928.0
17	23714.0
18	29512.0
19	15880.0
20	10147.0
21	8963.0
22	9087.0
23	9673.0
24	11166.0
25	13643.0
26	17180.0
27	21975.0
28	27581.0
29	35057.0
30	44163.0
31	55279.0
32	69708.0
33	89854.0
34	123630.0
35	194947.0
36	335391.0
37	763737.0
38	1548031.0
39	965542.0
40	8524.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.65999056014969	22.033954100618484	15.127382372213921	24.17867296701791
2	13.185891527763937	22.490231733876133	40.52284688910082	23.801029849259105
3	14.901358354903524	28.70133584687781	32.3728796972715	24.02442610094716
4	10.338425048409029	19.30064576511623	40.87719430915321	29.48373487732153
5	11.463562292908179	38.749395599922956	36.59675272018956	13.190289386979298
6	27.28572210184881	40.784872716791135	18.877277092580968	13.052128088779089
7	23.75173313251163	34.401407056723976	24.915784327811476	16.931075482952913
8	23.88827083528869	35.59143577879424	22.13625568647616	18.384037699440906
9	24.98611047477044	16.074728981890015	22.7515093877177	36.18765115562184
10-11	22.29218757019826	27.550401517372247	30.638691302021222	19.51871961040827
12-13	23.728013070610388	25.830046479311168	30.22323992247665	20.21870052760179
14-15	21.227075611061267	27.0791696265775	28.416634157689813	23.277120604671417
16-17	19.72901969977274	30.321812181591806	28.71381884322607	21.23534927540938
18-19	19.634183934234155	28.624965915616627	31.940781469838715	19.800068680310503
20-21	21.026683901984654	27.799478848110205	31.529989586748258	19.643847663156883
22-23	20.57469311245897	27.886237484128234	31.510660912759263	20.028408490653533
24-25	20.36674157823162	28.052637004593112	31.36053732598741	20.22008409118786
26-27	20.191115760494668	27.97255133835412	31.47201438200088	20.36431851915033
28-29	20.238049828970702	27.82694875482064	31.69571720042405	20.239284215784608
30-31	20.499454308118967	28.144144949642214	31.349381367563684	20.007019374675135
32-33	20.006209934716725	28.01884955398901	31.681020653482193	20.293919857812067
34-35	20.375831005607562	27.865716144198483	31.43622524415403	20.322227606039924
36-37	20.70593042140658	28.065943837663294	30.91618665144734	20.311939089482784
38-39	20.50973749796629	28.095592211780946	31.049564416793807	20.345105873458955
40-41	20.4111357104379	27.998649038780194	31.155937045296124	20.434278205485786
42-43	20.407399577707768	28.0374286991244	31.01204568193568	20.543126041232153
44-45	20.605900941921703	28.07228268527031	30.733686069765216	20.58813030304277
46-47	20.792745908858787	28.240606878562012	30.32065214490211	20.64599506767709
48-49	20.74320592853033	28.193458803862647	30.107933806470466	20.955401461136557
50-51	20.825795882018866	28.358558009341085	29.731707694088698	21.08393841455135
52-53	20.72892325474871	28.4305490855765	29.77911204221971	21.061415617455076
54-55	20.92078862082544	28.657236361946037	29.623156642913536	20.798818374314987
56-57	20.808562677936422	28.454436765922768	29.651590739527155	21.085409816613655
58-59	20.75718427969043	28.119170734387104	29.87000494420697	21.253640041715496
60-61	21.00660706265005	28.393265962712515	29.649878947064657	20.95024802757278
62-63	20.701964817354412	28.35214628194424	29.89098112759369	21.054907773107658
64-65	20.74271039016555	28.18828546988853	29.969124997720293	21.099879142225625
66-67	20.769991845271676	28.24163795608457	29.694009193836212	21.29436100480754
68-69	21.069356927046456	28.310253872768808	29.56798003278124	21.052409167403496
70-71	21.063158805465434	28.188828078310348	29.61652859549064	21.131484520733583
72-73	20.838660991047757	28.238435600266982	29.737904817655657	21.184998591029608
74-75	20.912179444407375	28.199850981962143	29.664253464113344	21.223716109517138
76-77	20.98834365802607	28.348882462197345	29.4410987640839	21.22167511569268
78-79	20.964892000149906	28.436133167991525	29.494329636718426	21.104645195140144
80-81	20.872853627000193	28.32880409851917	29.584875806813393	21.213466467667246
82-83	20.76375154910381	28.336389282559203	29.716570717867828	21.183288450469156
84-85	20.84503687268232	28.207462967736596	29.503328071791245	21.444172087789834
86-87	20.829538451136482	28.39338088028812	29.47327368723765	21.303806981337743
88-89	20.66361938034953	28.196450659861704	29.622554411497976	21.517375548290794
90-91	20.62478669138869	28.198961928673494	29.53662446997432	21.6396269099635
92-93	20.637371795506247	28.30872879837663	29.615475762988208	21.438423643128914
94-95	20.64187933607954	28.085440380984494	29.573916832234577	21.698763450701392
96-97	20.522507276043488	28.27691079616836	29.474484554970594	21.726097372817563
98-99	20.402028022257934	28.138769906259963	29.564915584146235	21.894286487335872
100	20.57426952429863	28.113575457003964	29.458792631887754	21.853362386809657
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2712.0
1	1657.5
2	1426.5
3	1950.0
4	1084.5
5	769.0
6	1882.0
7	4728.5
8	8438.5
9	9386.5
10	6073.5
11	3073.0
12	2665.0
13	2831.0
14	3176.5
15	3657.0
16	4148.0
17	4684.0
18	5335.5
19	6249.0
20	7704.0
21	9519.5
22	12133.0
23	16309.5
24	21845.0
25	28765.5
26	37993.0
27	49209.5
28	63065.5
29	80132.0
30	96028.0
31	110397.5
32	124665.0
33	135992.0
34	144668.0
35	153433.0
36	160808.5
37	165690.0
38	170958.0
39	173903.5
40	174591.5
41	173946.0
42	168930.0
43	164586.0
44	160262.5
45	152631.5
46	146477.5
47	141036.5
48	132151.0
49	126230.5
50	122968.0
51	116107.0
52	107187.0
53	99347.5
54	92781.5
55	86392.0
56	77444.0
57	67392.5
58	60540.0
59	54937.0
60	47885.5
61	39772.5
62	33093.5
63	27068.0
64	21312.5
65	16447.5
66	12632.5
67	9497.5
68	7034.5
69	5119.5
70	3565.5
71	2575.0
72	1899.0
73	1389.0
74	1085.0
75	777.0
76	468.5
77	296.5
78	211.0
79	141.0
80	68.5
81	26.0
82	14.5
83	9.0
84	8.0
85	5.0
86	4.5
87	3.5
88	3.0
89	2.0
90	1.0
91	2.0
92	3.0
93	3.5
94	4.0
95	7.5
96	11.5
97	10.5
98	4.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008006771949786863
2	4.44820663877048E-5
3	4.44820663877048E-4
4	0.002935816381588517
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.672309958155721E-5
24-25	7.784361617848339E-5
26-27	4.114591140862694E-4
28-29	7.673156451879078E-4
30-31	0.001490149223988111
32-33	0.003836578225939539
34-35	0.00468173748730593
36-37	0.0034696011782409746
38-39	0.002124018670012904
40-41	0.0030247805143639264
42-43	4.114591140862694E-4
44-45	3.0025394811700743E-4
46-47	0.0030692625807516314
48-49	0.0023353084853545017
50-51	7.673156451879078E-4
52-53	1.11205165969262E-5
54-55	3.447360145047122E-4
56-57	6.783515124124982E-4
58-59	5.115437634586052E-4
60-61	4.5594118047397416E-4
62-63	0.0
64-65	0.0
66-67	6.561104792186459E-4
68-69	5.449053132493838E-4
70-71	0.0018460057550897491
72-73	6.672309958155721E-5
74-75	0.0020906571202221258
76-77	3.3361549790778596E-4
78-79	0.0011898952758711034
80-81	7.450746119940555E-4
82-83	0.0010230875269172104
84-85	0.0010453285601110629
86-87	1.5568723235696679E-4
88-89	0.001390064574615775
90-91	0.001501269740585037
92-93	0.0023464290019514283
94-95	0.0011787747592741772
96-97	0.0023019469355637237
98-99	0.0019905724708497896
100	0.002602200883680731
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4496194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.453354355279956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86376719759824	43.914487252147104
2	16.087656247781233	19.129302552905422
3	4.999960119777256	8.917932022901557
4	2.0056438596782677	4.769690203997738
5	0.9819862728976033	2.919118892730092
6	0.5488887917989824	1.957996790427983
7	0.3338276202302836	1.389302025939161
8	0.2208694202811664	1.0505142328177155
9	0.1521624648480852	0.8141912047967435
>10	0.7231648707928009	7.642747958230666
>50	0.04963795467516894	2.038986259486556
>100	0.030112222188284674	3.2604885755365527
>500	0.0016860178361767166	0.6525585200367506
>1k	5.245385076359077E-4	0.5539003409296671
>5k	3.7467036259670626E-5	0.21740799997870003
>10k+	7.493407251934125E-5	0.7713751671375413
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19707	0.43830404115124927	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14922	0.3318806973186655	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9760	0.21707248397199944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	1.11205165969262E-5	0.0	0.0	2.22410331938524E-5	0.0
20-21	2.22410331938524E-5	0.0	0.0	4.44820663877048E-5	0.0
22-23	2.22410331938524E-5	0.0	0.0	1.445667157600406E-4	0.0
24-25	2.22410331938524E-5	0.0	0.0	2.446513651323764E-4	0.0
26-27	2.22410331938524E-5	0.0	0.0	4.670616970709004E-4	0.0
28-29	2.22410331938524E-5	0.0	0.0	0.0014567876741973323	0.0
30-31	4.44820663877048E-5	0.0	0.0	0.004248037340025809	0.0
32-33	6.67230995815572E-5	0.0	0.0	0.009919500804458171	0.0
34-35	6.67230995815572E-5	0.0	0.0	0.01933857836205466	0.0
36-37	6.67230995815572E-5	0.0	0.0	0.03407326285298188	0.0
38-39	6.67230995815572E-5	0.0	0.0	0.05365649258016891	0.0
40-41	6.67230995815572E-5	0.0	0.0	0.07352885573887603	0.0
42-43	6.67230995815572E-5	0.0	0.0	0.09281183151794606	0.0
44-45	6.67230995815572E-5	0.0	0.0	0.11928978153522735	0.0
46-47	6.67230995815572E-5	0.0	0.0	0.1525846082264244	0.0
48-49	6.67230995815572E-5	0.0	0.0	0.18690252244453864	0.0
50-51	6.67230995815572E-5	0.0	0.0	0.22162077526014223	0.0
52-53	6.67230995815572E-5	0.0	0.0	0.25598317154464423	0.0
54-55	6.67230995815572E-5	0.0	0.0	0.29621720059232315	0.0
56-57	6.67230995815572E-5	0.0	0.0	0.3448138581208907	0.0
58-59	6.67230995815572E-5	0.0	0.0	0.4005721283378787	0.0
60-61	6.67230995815572E-5	0.0	0.0	0.462302115967416	0.0
62-63	6.67230995815572E-5	0.0	0.0	0.5251441552566459	0.0
64-65	6.67230995815572E-5	0.0	0.0	0.5950922046513119	0.0
66-67	6.67230995815572E-5	0.0	0.0	0.6756492268794452	0.0
68-69	6.67230995815572E-5	0.0	0.0	0.7710966208308627	0.0
70-71	6.67230995815572E-5	0.0	0.0	0.8775199646634464	0.0
72-73	6.67230995815572E-5	0.0	0.0	0.9874685122572557	0.0
74-75	6.67230995815572E-5	0.0	0.0	1.1027437873009927	0.0
76-77	7.784361617848339E-5	0.0	0.0	1.2317529003419336	0.0
78-79	8.89641327754096E-5	0.0	0.0	1.3843152675351642	0.0
80-81	8.89641327754096E-5	0.0	0.0	1.5536473737565595	0.0
82-83	8.89641327754096E-5	0.0	0.0	1.7354678201163027	0.0
84-85	8.89641327754096E-5	0.0	0.0	1.9235602378367127	0.0
86-87	8.89641327754096E-5	0.0	0.0	2.126431822114437	0.0
88	8.89641327754096E-5	0.0	0.0	2.2947185997757216	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7640	0.0	60.294147	1
GTATCAA	15675	0.0	46.030243	1
ATCAACG	19825	0.0	36.034603	3
TCAACGC	19940	0.0	35.89749	4
CAACGCA	20785	0.0	34.46033	5
TATCAAC	21100	0.0	34.12445	2
AACGCAG	21535	0.0	33.30383	6
ACGCAGA	24440	0.0	29.364483	7
CGCAGAG	24530	0.0	29.141787	8
GTGGTAT	2910	0.0	26.49069	1
GCAGAGT	27200	0.0	25.901043	9
TGGTATC	3015	0.0	24.629698	2
GTACATG	15805	0.0	24.000566	1
TACATGG	15680	0.0	23.079903	2
ACATGGG	15790	0.0	22.383347	3
AGAGTAC	25595	0.0	21.860434	10-11
GAGTACT	18265	0.0	21.704508	12-13
CAGAGTA	26865	0.0	21.141914	10-11
AGTACTT	19115	0.0	20.554953	12-13
CATGGGT	4615	0.0	19.756926	4
>>END_MODULE