FastQCFastQC Report
Wed 25 May 2016
SRR2431377_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431377_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297107
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA224690.9781433777355605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2200.051.2723852
ATAACGC2650.042.565753
TACCTGG24000.041.1247252
GGTATCA32500.040.926771
GTACCTG26950.037.6703261
GTATCAA53200.033.8369751
TAACGCA3300.032.7573554
ACCTGGG30200.032.0593873
TCTGTCG7400.031.1214148
TATCACG3450.028.6085032
TCAACGC61250.027.931244
CAACGCA62150.027.5267665
ATCAACG62350.027.4384693
AACGCAG65500.026.405936
CTGTCGC9050.025.4473429
GTATAAC6700.025.2541121
TATCAAC70000.024.8426912
GTACATG88000.023.8742221
TACATGG87800.023.232192
ACGCAGA73900.023.0864497
Produced by FastQC (version 0.11.5)