##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431377_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297107 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0792718841569 33.0 31.0 34.0 28.0 34.0 2 31.17625865926141 34.0 31.0 34.0 27.0 34.0 3 31.24146197804456 34.0 31.0 34.0 27.0 34.0 4 34.37242975621075 37.0 35.0 37.0 31.0 37.0 5 34.534976385514476 37.0 35.0 37.0 31.0 37.0 6 34.60353392332181 37.0 35.0 37.0 31.0 37.0 7 34.5508372052325 37.0 35.0 37.0 31.0 37.0 8 34.52614919548807 37.0 35.0 37.0 31.0 37.0 9 36.033479938026396 39.0 37.0 39.0 31.0 39.0 10-11 36.0555805628558 39.0 37.0 39.0 31.0 39.0 12-13 36.006099628793955 39.0 37.0 39.0 31.0 39.0 14-15 37.40120965196658 41.0 38.0 41.0 31.0 41.0 16-17 37.41018942522051 41.0 38.0 41.0 31.0 41.0 18-19 37.43374296452016 41.0 38.5 41.0 31.0 41.0 20-21 37.48617674318175 41.0 38.5 41.0 31.0 41.0 22-23 37.49785904618287 41.0 38.0 41.0 31.0 41.0 24-25 37.529615947363354 41.0 38.0 41.0 30.5 41.0 26-27 37.54139989996112 41.0 38.0 41.0 30.0 41.0 28-29 37.51739514093161 41.0 38.0 41.0 30.0 41.0 30-31 37.456953463639266 41.0 38.0 41.0 30.0 41.0 32-33 37.37053868191599 40.0 38.0 41.0 30.0 41.0 34-35 37.310064354860266 40.0 38.0 41.0 30.0 41.0 36-37 37.21484915591655 40.0 38.0 41.0 30.0 41.0 38-39 37.111186592527034 40.0 38.0 41.0 30.0 41.0 40-41 37.02478008207716 40.0 38.0 41.0 30.0 41.0 42-43 36.911658011577174 40.0 37.5 41.0 30.0 41.0 44-45 36.79429256016372 40.0 37.0 41.0 29.5 41.0 46-47 36.68532789286698 40.0 37.0 41.0 29.0 41.0 48-49 36.56381548617456 40.0 37.0 41.0 29.0 41.0 50-51 36.44536323297086 40.0 36.5 41.0 29.0 41.0 52-53 36.285676505273806 40.0 36.0 41.0 29.0 41.0 54-55 36.132965943684816 40.0 36.0 41.0 29.0 41.0 56-57 35.95762517810446 40.0 35.0 41.0 28.5 41.0 58-59 35.746364231182966 39.0 35.0 41.0 28.0 41.0 60-61 35.574861336454944 39.0 35.0 41.0 28.0 41.0 62-63 35.39332669309701 39.0 35.0 41.0 28.0 41.0 64-65 35.1227574510025 38.5 35.0 41.0 28.0 41.0 66-67 34.81990303455608 38.0 34.5 40.0 27.0 41.0 68-69 34.486578335271275 37.0 34.0 40.0 26.0 41.0 70-71 34.21043403724772 37.0 34.0 40.0 26.0 41.0 72-73 33.94637298131954 36.5 34.0 39.0 26.0 41.0 74-75 33.606610184027126 36.0 34.0 39.0 26.0 41.0 76-77 33.25633111561629 35.5 34.0 39.0 25.5 40.5 78-79 32.913307042292764 35.0 33.5 37.5 25.0 40.0 80-81 32.56911301911491 35.0 33.0 37.0 25.0 39.0 82-83 32.239280103190666 35.0 33.0 37.0 24.0 39.0 84-85 31.941203653116727 35.0 33.0 36.0 24.0 38.5 86-87 31.666609130528094 35.0 33.0 36.0 23.0 37.0 88-89 31.409632420257307 35.0 33.0 36.0 22.5 37.0 90-91 31.192029147967425 35.0 32.5 35.0 20.5 36.5 92-93 30.97226924997399 35.0 32.0 35.0 20.0 36.0 94-95 30.7513944713938 35.0 32.0 35.0 18.5 36.0 96-97 30.556683036532473 35.0 32.0 35.0 15.0 36.0 98-99 30.427631581811383 35.0 32.0 35.0 2.0 36.0 100 30.3730379124699 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3738976135506 1101 2 0.4604184632025792 1101 3 0.44480136029138606 1101 4 0.37066185670984453 1101 5 0.43059439682918565 1101 6 0.38484592548818597 1101 7 0.3965020362410172 1101 8 0.40870709947154893 1101 9 0.4603010985128151 1101 10-11 0.40559896229515857 1101 12-13 0.4633462112329383 1101 14-15 0.5411859441896709 1101 16-17 0.4998411892836856 1101 18-19 0.5833782762196691 1101 20-21 0.5595220485515355 1101 22-23 0.544585680444122 1101 24-25 0.5724693412494659 1101 26-27 0.5161564839285333 1101 28-29 0.5367265791384384 1101 30-31 0.5784326506810302 1101 32-33 0.5805485140763764 1101 34-35 0.6009332420447961 1101 36-37 0.5672587591687943 1101 38-39 0.549374046905676 1101 40-41 0.5494552076966102 1101 42-43 0.5521489992210533 1101 44-45 0.6341198872746716 1101 46-47 0.579084315123275 1101 48-49 0.5388073683638126 1101 50-51 0.5623869973850759 1101 52-53 0.6209481177284033 1101 54-55 0.6732023156173526 1101 56-57 0.6208928191761487 1101 58-59 0.8024200193930753 1101 60-61 0.7850641829920804 1101 62-63 0.7540513932738477 1101 64-65 0.7458884002253754 1101 66-67 0.6368864770487406 1101 68-69 0.6137344096378214 1101 70-71 0.5769631757813869 1101 72-73 0.6223557363125778 1101 74-75 0.6169756831808186 1101 76-77 0.4969496850927797 1101 78-79 0.4264060717945384 1101 80-81 0.5117037293947533 1101 82-83 0.4986129453635897 1101 84-85 0.4934463395539872 1101 86-87 0.443717501737531 1101 88-89 0.5278523411255733 1101 90-91 0.511778001935344 1101 92-93 0.4574859131419977 1101 94-95 0.4994893949760879 1101 96-97 0.6227978576829081 1101 98-99 0.6368276729981339 1101 100 0.635122198865993 1102 1 0.20116002269148225 1102 2 0.27997452468841644 1102 3 0.28319997613961334 1102 4 0.14898546131384904 1102 5 0.3565201951882244 1102 6 0.3087596750907764 1102 7 0.29487089902589503 1102 8 0.29905656545582815 1102 9 0.4358847959619112 1102 10-11 0.41867440895706665 1102 12-13 0.46021079250626684 1102 14-15 0.5536649040245649 1102 16-17 0.5140358440460631 1102 18-19 0.40846187478849316 1102 20-21 0.3892669412437044 1102 22-23 0.35836085700545794 1102 24-25 0.407974309517364 1102 26-27 0.4187106568822543 1102 28-29 0.45879920366995464 1102 30-31 0.4702073417865762 1102 32-33 0.44538543144929577 1102 34-35 0.4431798652615484 1102 36-37 0.4668819712271528 1102 38-39 0.48051749048262593 1102 40-41 0.5059173141595323 1102 42-43 0.4561261967672152 1102 44-45 0.4403975750699587 1102 46-47 0.4285956015878227 1102 48-49 0.4022099236370309 1102 50-51 0.3675930276377599 1102 52-53 0.3110583575257664 1102 54-55 0.3582613371493295 1102 56-57 0.3850056955897614 1102 58-59 0.40616505157963445 1102 60-61 0.341636813407618 1102 62-63 0.4054730831977196 1102 64-65 0.399811326033209 1102 66-67 0.35015053773435056 1102 68-69 0.39436062847965303 1102 70-71 0.3884375559330735 1102 72-73 0.38204775029684157 1102 74-75 0.3752999728190147 1102 76-77 0.39121255242204 1102 78-79 0.38863882593911825 1102 80-81 0.3929226271919575 1102 82-83 0.38026797840835513 1102 84-85 0.3831664542914801 1102 86-87 0.38281882298288394 1102 88-89 0.33307879802634943 1102 90-91 0.36178445522534375 1102 92-93 0.30243373334118573 1102 94-95 0.36433104826975793 1102 96-97 0.3917490751159214 1102 98-99 0.39624404682703585 1102 100 0.4036633046967921 1103 1 0.21456698066242907 1103 2 0.2125267125235979 1103 3 0.1948944896119258 1103 4 0.12561148979091996 1103 5 0.12258909101138471 1103 6 0.1589388613232856 1103 7 0.15456352799446904 1103 8 0.19753913541821788 1103 9 0.25127718051363956 1103 10-11 0.17727188479827305 1103 12-13 0.17517798065534151 1103 14-15 0.2444019747279924 1103 16-17 0.25476278239344197 1103 18-19 0.255856475864654 1103 20-21 0.3024464364081254 1103 22-23 0.29495959490697743 1103 24-25 0.33337197586975975 1103 26-27 0.3597862360532531 1103 28-29 0.38933842056286494 1103 30-31 0.3546830744122076 1103 32-33 0.29537237006000083 1103 34-35 0.3908688580309345 1103 36-37 0.4425143227905295 1103 38-39 0.381883011506126 1103 40-41 0.35368653572552944 1103 42-43 0.3886115034198241 1103 44-45 0.38682608175721356 1103 46-47 0.3891486316870285 1103 48-49 0.38124725249610236 1103 50-51 0.31286194246624177 1103 52-53 0.29837869663721506 1103 54-55 0.3189740806574264 1103 56-57 0.26869993924754 1103 58-59 0.3585567646481067 1103 60-61 0.3494942074610492 1103 62-63 0.3271219686290081 1103 64-65 0.37648124230484115 1103 66-67 0.298087019501736 1103 68-69 0.3985229564561976 1103 70-71 0.47516646884957936 1103 72-73 0.40676516537443064 1103 74-75 0.3939795245605282 1103 76-77 0.41678210627589607 1103 78-79 0.3663098609678741 1103 80-81 0.3695648750713616 1103 82-83 0.35014129724345366 1103 84-85 0.3633346094222354 1103 86-87 0.38736154364832487 1103 88-89 0.3427171778645288 1103 90-91 0.28498339077458823 1103 92-93 0.312653101711863 1103 94-95 0.3266295722850643 1103 96-97 0.2590482698512169 1103 98-99 0.2568010745318752 1103 100 0.27554290635590206 1104 1 0.20002905848327046 1104 2 0.2404205542637996 1104 3 0.2864008497426731 1104 4 0.18702442948710996 1104 5 0.22812514582820853 1104 6 0.33225751585079166 1104 7 0.227630011998194 1104 8 0.24103537434791633 1104 9 0.2850808484960936 1104 10-11 0.2810293302376152 1104 12-13 0.3220394703523368 1104 14-15 0.3865602113579598 1104 16-17 0.3808968116994862 1104 18-19 0.3951752716495065 1104 20-21 0.28219908283463724 1104 22-23 0.22412258533250196 1104 24-25 0.24808925377340785 1104 26-27 0.2748802002164936 1104 28-29 0.3301799929738891 1104 30-31 0.33143213372422764 1104 32-33 0.29268091310257915 1104 34-35 0.29437514192756 1104 36-37 0.3179458957508814 1104 38-39 0.3141776735852915 1104 40-41 0.36706543763277466 1104 42-43 0.330721495807623 1104 44-45 0.3370114726831801 1104 46-47 0.3554394155419871 1104 48-49 0.2975916991951806 1104 50-51 0.1685838179602186 1104 52-53 0.11913965893607781 1104 54-55 0.1680027682309202 1104 56-57 0.19576733208161556 1104 58-59 0.17507795119487213 1104 60-61 0.19032763328431201 1104 62-63 0.1982042727082458 1104 64-65 0.21499774867431398 1104 66-67 0.2972182681307345 1104 68-69 0.2603090883479595 1104 70-71 0.19453688709464245 1104 72-73 0.1770401725027 1104 74-75 0.2135215816935201 1104 76-77 0.19441865784771295 1104 78-79 0.17679425782328906 1104 80-81 0.26696009706027723 1104 82-83 0.23381409664644082 1104 84-85 0.3310062959564881 1104 86-87 0.25669933817270163 1104 88-89 0.21530295713609604 1104 90-91 0.17395464864137367 1104 92-93 0.1770862206554078 1104 94-95 0.21405223108466842 1104 96-97 0.22139898269633207 1104 98-99 0.16795263208097566 1104 100 0.25506451480623227 1105 1 0.16029773368833844 1105 2 0.19017352305163726 1105 3 0.18681188946155203 1105 4 0.1823772703271871 1105 5 0.09427564588818171 1105 6 0.14907551695373655 1105 7 0.2471961740407167 1105 8 0.09775538527176053 1105 9 0.2387927668750791 1105 10-11 0.19784782170638238 1105 12-13 0.25140576305848583 1105 14-15 0.21348210589007977 1105 16-17 0.23027095403080722 1105 18-19 0.13600627951723965 1105 20-21 0.09887138870823975 1105 22-23 0.1144184154878829 1105 24-25 0.17711021578062258 1105 26-27 0.13632262259346817 1105 28-29 0.10747328303431658 1105 30-31 0.08742440405008267 1105 32-33 0.13367196883000787 1105 34-35 0.1475721573348565 1105 36-37 0.14850394714272852 1105 38-39 0.17205683386777082 1105 40-41 0.16265380494845516 1105 42-43 0.10361997650343113 1105 44-45 0.15799230239723983 1105 46-47 0.1579983329953123 1105 48-49 0.12214305218991939 1105 50-51 0.15889041730343223 1105 52-53 0.17317618062797635 1105 54-55 0.17801896161913078 1105 56-57 0.15298706349693703 1105 58-59 0.14986682984894628 1105 60-61 0.19333923383081242 1105 62-63 0.22650865936999054 1105 64-65 0.3400636651567126 1105 66-67 0.22661002213909853 1105 68-69 0.24211415888142085 1105 70-71 0.2443884194123882 1105 72-73 0.16308021200355682 1105 74-75 0.1978266692011914 1105 76-77 0.21298475651988014 1105 78-79 0.10684960880828243 1105 80-81 0.19044586626696258 1105 82-83 0.2793061825193064 1105 84-85 0.2615088228492759 1105 86-87 0.27737731041196056 1105 88-89 0.3588100233075693 1105 90-91 0.2557784096566884 1105 92-93 0.27247491478105346 1105 94-95 0.29220117178133265 1105 96-97 0.29845421901116254 1105 98-99 0.29017150347009135 1105 100 0.3732343790352495 1106 1 0.06858135892868233 1106 2 0.03928928724164393 1106 3 0.14280967277096224 1106 4 0.07864758687360052 1106 5 0.069458472984536 1106 6 0.05759312026302865 1106 7 -0.02688138946647456 1106 8 0.03193371165254888 1106 9 0.003201968242997566 1106 10-11 0.03546223226156542 1106 12-13 0.01163698184285522 1106 14-15 0.07033718235022945 1106 16-17 0.06911118878197442 1106 18-19 0.05439838970906408 1106 20-21 0.08635970761812217 1106 22-23 0.08296849427623698 1106 24-25 0.07112140148208113 1106 26-27 0.0890816755941799 1106 28-29 0.17689284819672224 1106 30-31 0.15660023834622194 1106 32-33 0.08187504041786298 1106 34-35 0.06730765969030728 1106 36-37 0.12098101871703903 1106 38-39 0.0810810482376425 1106 40-41 0.14237288519643698 1106 42-43 0.13699890305088758 1106 44-45 0.13512043402491258 1106 46-47 0.14087625077397803 1106 48-49 0.1225115654218385 1106 50-51 0.09798566766442462 1106 52-53 0.10552129284660339 1106 54-55 0.15238678440644549 1106 56-57 0.14404263502510872 1106 58-59 0.193894653199159 1106 60-61 0.1707577227119117 1106 62-63 0.15088854178064537 1106 64-65 0.15252351836776512 1106 66-67 0.23609744539483302 1106 68-69 0.21197093437683634 1106 70-71 0.21507658747900393 1106 72-73 0.1928692630055977 1106 74-75 0.1852889361182406 1106 76-77 0.2006944951021623 1106 78-79 0.22333078528599515 1106 80-81 0.17345774027542404 1106 82-83 0.21999755164103618 1106 84-85 0.1262334270104013 1106 86-87 0.17515695027633882 1106 88-89 0.1688420979867402 1106 90-91 0.0843887170897979 1106 92-93 0.15768604903761485 1106 94-95 0.1631465019638476 1106 96-97 0.08511159958971959 1106 98-99 0.12762759806884105 1106 100 0.07318311227602692 1107 1 0.09651578806434458 1107 2 0.19915063450791948 1107 3 0.17420859348890616 1107 4 0.0376874795172526 1107 5 0.058464629327303896 1107 6 0.12148836839750743 1107 7 0.22201786801085177 1107 8 0.16219104983013466 1107 9 0.212868201570231 1107 10-11 0.1899176383298382 1107 12-13 0.12421639828332331 1107 14-15 0.1995128795692409 1107 16-17 0.24168397169798084 1107 18-19 0.20866301840960233 1107 20-21 0.23257157756037117 1107 22-23 0.2456240310452742 1107 24-25 0.17842132034213165 1107 26-27 0.20718128520745438 1107 28-29 0.18646519396092032 1107 30-31 0.18797751591982603 1107 32-33 0.2554302632382033 1107 34-35 0.2619095894486563 1107 36-37 0.28212800846938535 1107 38-39 0.256803007935801 1107 40-41 0.29668818665326313 1107 42-43 0.1917847304369218 1107 44-45 0.27342659945382763 1107 46-47 0.24285747612065478 1107 48-49 0.19877640666202723 1107 50-51 0.19696064040596895 1107 52-53 0.17600808965488568 1107 54-55 0.25237564910796095 1107 56-57 0.1819995079028871 1107 58-59 0.19780848822772867 1107 60-61 0.19915495772674774 1107 62-63 0.2325770358562238 1107 64-65 0.2515261307945593 1107 66-67 0.17461876353459616 1107 68-69 0.16089220035066631 1107 70-71 0.19032513020043496 1107 72-73 0.21619155793820255 1107 74-75 0.22177733158050472 1107 76-77 0.185015049687685 1107 78-79 0.175695257107499 1107 80-81 0.20966195529565823 1107 82-83 0.26080230490443057 1107 84-85 0.22948794435160025 1107 86-87 0.17856727442948284 1107 88-89 0.23509144511831437 1107 90-91 0.195552632178142 1107 92-93 0.28724941666957093 1107 94-95 0.3315205148776137 1107 96-97 0.32693307232110413 1107 98-99 0.3169352175993616 1107 100 0.27850554667784166 1108 1 0.09302553257405677 1108 2 0.10200341094207843 1108 3 0.09888179742735304 1108 4 0.09554242825463177 1108 5 0.13184040412583897 1108 6 0.14582104744266644 1108 7 0.10872765764962367 1108 8 0.15744462961132655 1108 9 0.09382177723744434 1108 10-11 0.06305754525428142 1108 12-13 0.09988227778874403 1108 14-15 0.11763165718273427 1108 16-17 0.11767759784241605 1108 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-0.07534707891060322 2215 6 -0.15767095471299797 2215 7 -0.14868726994362902 2215 8 -0.20539848191192078 2215 9 -0.15448816294451007 2215 10-11 -0.20226796054647878 2215 12-13 -0.172224977772359 2215 14-15 -0.289849494732934 2215 16-17 -0.24138512408286772 2215 18-19 -0.21593226590903924 2215 20-21 -0.20802949064746912 2215 22-23 -0.13321875497318558 2215 24-25 -0.17648072705623719 2215 26-27 -0.12532300909543892 2215 28-29 -0.15373781249842722 2215 30-31 -0.20343672998269113 2215 32-33 -0.24950427100886685 2215 34-35 -0.2683878916743012 2215 36-37 -0.2864153282039581 2215 38-39 -0.182888037100696 2215 40-41 -0.20576482204269553 2215 42-43 -0.11158875761060472 2215 44-45 -0.08848108869031535 2215 46-47 -0.11962418362741545 2215 48-49 -0.11924272311538431 2215 50-51 -0.15360237177910818 2215 52-53 -0.09041733932808427 2215 54-55 -0.08593322310073859 2215 56-57 -0.048711076211766624 2215 58-59 -0.024406309671647364 2215 60-61 -0.0338590613828984 2215 62-63 -0.1132415544493881 2215 64-65 -0.055138817405406826 2215 66-67 -0.16800006787822497 2215 68-69 -0.2307020931470376 2215 70-71 -0.16911333378109816 2215 72-73 -0.14771957906795308 2215 74-75 -0.17695512216627662 2215 76-77 -0.14623869664426792 2215 78-79 -0.1370135869198421 2215 80-81 -0.038395264053754374 2215 82-83 -0.06679261708240603 2215 84-85 -0.15462319086525866 2215 86-87 -0.11734486234406205 2215 88-89 -0.18534289454315456 2215 90-91 -0.28598549988956634 2215 92-93 -0.274039735295073 2215 94-95 -0.17843550206698566 2215 96-97 -0.24340766495702226 2215 98-99 -0.20876629703705518 2215 100 -0.18683193632387685 2216 1 -0.33401088228551856 2216 2 -0.32688163895445044 2216 3 -0.4557508860799473 2216 4 -0.38433040302695076 2216 5 -0.3390368577095515 2216 6 -0.4048902629358935 2216 7 -0.3995340322211476 2216 8 -0.4229951261249525 2216 9 -0.5244065202850834 2216 10-11 -0.5095592263402793 2216 12-13 -0.5160105812220337 2216 14-15 -0.5984671449218908 2216 16-17 -0.6058846304450469 2216 18-19 -0.6814368882404693 2216 20-21 -0.6932636502896585 2216 22-23 -0.6233070990392235 2216 24-25 -0.6345252537578219 2216 26-27 -0.6748337185180731 2216 28-29 -0.5746393595469996 2216 30-31 -0.5546069461856007 2216 32-33 -0.6513075218918587 2216 34-35 -0.6746547283348221 2216 36-37 -0.6401322452053364 2216 38-39 -0.6330559167195844 2216 40-41 -0.7116028375711494 2216 42-43 -0.725800882666789 2216 44-45 -0.681619178525743 2216 46-47 -0.6149188269681503 2216 48-49 -0.7002270008220535 2216 50-51 -0.6618713397767451 2216 52-53 -0.6891267306094591 2216 54-55 -0.7859680723155265 2216 56-57 -0.7749083736040845 2216 58-59 -0.7380747560950809 2216 60-61 -0.7051468123233775 2216 62-63 -0.7643775153232468 2216 64-65 -0.7781145222527499 2216 66-67 -0.8087569847465801 2216 68-69 -0.8989208801758153 2216 70-71 -0.9369078725979492 2216 72-73 -0.8897531364624953 2216 74-75 -0.9444803338033978 2216 76-77 -0.9212245384084028 2216 78-79 -0.8577925084423583 2216 80-81 -0.8043355728363224 2216 82-83 -0.8017797202598373 2216 84-85 -0.7963877536560844 2216 86-87 -0.8126737610138548 2216 88-89 -0.7815697840737528 2216 90-91 -0.7575058877492111 2216 92-93 -0.8593540932665853 2216 94-95 -0.792037231367221 2216 96-97 -0.888216486013075 2216 98-99 -0.8958437473383221 2216 100 -0.9481663643073936 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40965.0 3 8993.0 4 6460.0 5 6248.0 6 3851.0 7 3600.0 8 4006.0 9 4227.0 10 4507.0 11 4901.0 12 6070.0 13 6700.0 14 7293.0 15 7463.0 16 7154.0 17 6929.0 18 6533.0 19 6033.0 20 5597.0 21 5898.0 22 7329.0 23 12634.0 24 21453.0 25 25929.0 26 23753.0 27 17479.0 28 18530.0 29 22010.0 30 25163.0 31 29930.0 32 38834.0 33 51838.0 34 73443.0 35 121458.0 36 200425.0 37 413713.0 38 670734.0 39 362298.0 40 6726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.01358234295416 22.194810848461103 13.063645466022376 21.72796134256236 2 16.043135996712387 23.238316717505977 39.37813954682999 21.340407738951647 3 16.480947557079404 30.024635334792848 30.691299969918685 22.80311713820906 4 11.656270256457361 20.230402850193745 39.56446086316397 28.54886603018492 5 11.79344279565558 38.9853411269044 33.93934196360901 15.281874113831007 6 26.784864614491184 41.44839574299325 18.936166229957944 12.830573412557623 7 23.625238180023832 35.24864100801574 24.29203341420317 16.834087397757266 8 22.669253108366306 37.17593477360872 22.567777643792823 17.587034474232155 9 23.63577317033991 19.00272821422772 22.59150313851292 34.76999547691945 10-11 21.835486984280664 29.41983111801061 29.19735563036463 19.547326267344097 12-13 22.65253642951765 28.327174136859973 29.01802136339317 20.002268070229206 14-15 21.01856813809718 29.095706033719804 26.60294013295854 23.282785695224472 16-17 20.21268491193488 32.48457734010649 26.4277197361725 20.87501801178613 18-19 19.49164753753308 30.83648258439855 28.98968136878256 20.682188509285808 20-21 22.48817752068145 30.22789099506466 27.194292647229755 20.089638837024136 22-23 20.91371886464148 31.120535525772198 28.013279311760403 19.952466297825918 24-25 21.03025675338589 29.810779384678206 28.07185298725745 21.087110874678455 26-27 20.963433571009098 30.14670191680231 28.057225893264874 20.832638618923717 28-29 20.54995696761187 30.06925667807377 28.41804931159062 20.962737042723738 30-31 21.8211210883951 30.85763963106638 26.991385251100624 20.329854029437897 32-33 19.967006325782823 30.647745185574728 28.730006917396533 20.65524157124592 34-35 20.516501843405642 29.028338688620074 28.659026331816502 21.796133136157785 36-37 21.655477955532763 30.63137677086875 27.390691857192547 20.32245341640594 38-39 20.884595275709838 30.180461772133384 28.240369299296898 20.69457365285988 40-41 20.92832419212514 29.89688769395592 27.99612730273339 21.178660811185548 42-43 20.029476206376106 30.43327977321039 28.649949697597894 20.88729432281561 44-45 20.979714919679402 29.70390582589318 28.208764328348657 21.10761492607876 46-47 20.945193236536216 31.093414455660966 27.573661131153226 20.387731176649584 48-49 20.149322604475977 30.107130403590254 28.747311291985962 20.996235699947803 50-51 21.027840670896044 29.605042342389797 28.012909281108804 21.354207705605354 52-53 20.254759573672448 30.36321338100489 28.394737380539958 20.9872896647827 54-55 20.935267708469826 30.272229373729655 27.90274897947723 20.88975393832329 56-57 20.544863604525172 30.50606697903058 27.75582504428396 21.193244372160287 58-59 19.845788637621148 29.645223317851542 28.771428583866577 21.737559460660734 60-61 21.532801911273616 30.315631792511187 27.264489638488758 20.887076657726436 62-63 20.447349644574675 30.55005709355289 28.178813612078145 20.823779649794286 64-65 20.68896690009081 29.346229560150906 28.378816650167643 21.585986889590643 66-67 20.767077894064144 30.164376322043335 27.64734076383904 21.421205020053485 68-69 21.05665952870284 30.181745996159513 27.93888138428031 20.822713090857327 70-71 21.839970885117673 29.657891426041537 27.60217525783518 20.89996243100561 72-73 20.587852459637272 30.25714518304981 28.720799684124422 20.43420267318849 74-75 20.74548551721796 29.41212577385381 28.355666497033006 21.486722211895223 76-77 21.160376943694832 30.38815780022437 27.610664196313017 20.840801059767784 78-79 20.969245228890077 30.27838929575331 28.09364126268389 20.658724212672723 80-81 21.2972447517682 29.82858438897274 27.740414355970355 21.1337565032887 82-83 20.315962918543125 30.34206098545778 28.50446540319228 20.837510692806816 84-85 20.89045367231331 29.581698563942382 27.984937447963997 21.542910315780308 86-87 21.368195021717813 30.34529744860589 27.69651872868895 20.589988800987342 88-89 20.337762240806605 29.962865091008823 28.317044183140837 21.38232848504373 90-91 20.914497464784073 29.7292432052861 27.665307092640408 21.69095223728942 92-93 20.566846686751774 30.590035038289187 28.081827258644044 20.761291016314996 94-95 20.783322859802777 29.540710834564436 28.01950579882419 21.656460506808592 96-97 20.706324145000035 30.285889167529906 27.98899811781269 21.018788569657364 98-99 20.181110436574887 29.952593574850194 28.40212768412027 21.464168304454645 100 21.204227001508897 29.943205217841033 27.516170797051164 21.336396983598906 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 19932.0 1 12854.0 2 4825.5 3 3016.0 4 1701.0 5 1124.0 6 1004.5 7 1160.0 8 1566.0 9 1673.5 10 1193.5 11 757.0 12 658.0 13 687.0 14 742.5 15 811.0 16 963.0 17 1218.0 18 1565.0 19 2047.0 20 2798.0 21 3902.0 22 5503.5 23 7952.5 24 11079.0 25 14554.0 26 19237.0 27 25375.5 28 32945.0 29 41282.0 30 48437.0 31 55366.5 32 61724.5 33 66811.5 34 71467.5 35 75754.0 36 79086.5 37 81577.5 38 84381.0 39 86622.0 40 88248.0 41 87840.0 42 84675.0 43 81729.5 44 77704.5 45 72382.5 46 68070.5 47 64560.0 48 61607.0 49 60738.5 50 60922.0 51 58701.5 52 54366.0 53 50927.0 54 48953.5 55 47178.5 56 44213.5 57 40373.0 58 38010.0 59 35399.0 60 31869.5 61 27386.5 62 22069.0 63 16895.5 64 12420.0 65 9191.5 66 6682.5 67 4848.5 68 3487.5 69 2549.0 70 1841.0 71 1337.0 72 1044.5 73 772.5 74 599.0 75 454.5 76 319.0 77 262.0 78 190.0 79 123.5 80 101.0 81 84.0 82 77.0 83 63.0 84 60.0 85 58.5 86 48.0 87 40.0 88 31.5 89 25.5 90 25.0 91 20.5 92 17.5 93 18.5 94 12.0 95 10.0 96 13.0 97 10.5 98 9.5 99 10.5 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.0473112484529456E-4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.353301783504208E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.706603567008416E-5 84-85 8.488938477833205E-4 86-87 0.0012842240261337413 88-89 0.0033520423732982404 90-91 0.0027861131414426933 92-93 0.0021113513649995406 94-95 0.0020242853293294567 96-97 0.0019807523114944146 98-99 0.0024160824898448352 100 0.002829646159277735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2297107.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.47528425252024 #Duplication Level Percentage of deduplicated Percentage of total 1 84.36116580933594 61.98460637714951 2 11.029870090949478 16.208456804017686 3 2.4239583369949202 5.34303083480904 4 0.805411257839167 2.367112844396507 5 0.3748907388101781 1.3772601798857578 6 0.2221922345157312 0.9795382553847595 7 0.14846139790238982 0.7635770387983225 8 0.09974204383611911 0.5862860018228949 9 0.08000729169601206 0.529070264967491 >10 0.4118246150464776 5.519907124579035 >50 0.02970490304197654 1.4857495007819015 >100 0.012000511177182704 1.4689550094588415 >500 5.928991187226485E-4 0.3004569855460262 >1k 1.1857982374373936E-4 0.10716604095107549 >5k 0.0 0.0 >10k+ 5.928991187186968E-5 0.9788267374511037 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22469 0.9781433777355605 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 4.3533017835042076E-5 0.0 0.0 6.529952675256312E-5 0.0 14-15 4.3533017835042076E-5 0.0 0.0 1.3059905350512623E-4 0.0 16-17 4.3533017835042076E-5 0.0 0.0 1.3059905350512623E-4 4.3533017835042076E-5 18-19 4.3533017835042076E-5 0.0 0.0 2.3943159809273142E-4 4.3533017835042076E-5 20-21 4.3533017835042076E-5 0.0 0.0 3.264976337628156E-4 4.3533017835042076E-5 22-23 4.3533017835042076E-5 0.0 0.0 5.65929231855547E-4 4.3533017835042076E-5 24-25 4.3533017835042076E-5 0.0 0.0 0.0011318584637110942 4.3533017835042076E-5 26-27 4.3533017835042076E-5 0.0 0.0 0.0020242853293294567 4.3533017835042076E-5 28-29 4.3533017835042076E-5 0.0 0.0 0.003809139060566182 4.3533017835042076E-5 30-31 4.3533017835042076E-5 0.0 0.0 0.0071394149249469005 4.3533017835042076E-5 32-33 4.3533017835042076E-5 0.0 0.0 0.013190504404017749 4.3533017835042076E-5 34-35 4.3533017835042076E-5 0.0 0.0 0.023333697559582554 4.3533017835042076E-5 36-37 4.3533017835042076E-5 0.0 0.0 0.039288548596125475 4.3533017835042076E-5 38-39 4.3533017835042076E-5 0.0 0.0 0.06216514946844009 4.3533017835042076E-5 40-41 4.3533017835042076E-5 0.0 0.0 0.09024394597204222 4.3533017835042076E-5 42-43 4.3533017835042076E-5 0.0 0.0 0.11936753490368537 4.3533017835042076E-5 44-45 4.3533017835042076E-5 2.1766508917521038E-5 0.0 0.15336682183285325 4.3533017835042076E-5 46-47 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.19280773599140136 4.3533017835042076E-5 48-49 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.23562245903216522 4.3533017835042076E-5 50-51 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.28294284941885595 4.3533017835042076E-5 52-53 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.3299802751896189 4.3533017835042076E-5 54-55 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.3810009720922882 4.3533017835042076E-5 56-57 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.4414901003740792 4.3533017835042076E-5 58-59 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.5075079219209205 4.3533017835042076E-5 60-61 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.5813616866780694 4.3533017835042076E-5 62-63 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.6563255433900119 4.3533017835042076E-5 64-65 4.3533017835042076E-5 4.3533017835042076E-5 0.0 0.7458729610766934 4.3533017835042076E-5 66-67 4.3533017835042076E-5 8.706603567008415E-5 0.0 0.8506134019878047 4.3533017835042076E-5 68-69 4.3533017835042076E-5 8.706603567008415E-5 0.0 0.9619055620830897 4.3533017835042076E-5 70-71 4.3533017835042076E-5 8.706603567008415E-5 0.0 1.0918080873028553 4.3533017835042076E-5 72-73 4.3533017835042076E-5 8.706603567008415E-5 0.0 1.2382096262821018 4.3533017835042076E-5 74-75 4.3533017835042076E-5 8.706603567008415E-5 0.0 1.38635248597475 4.3533017835042076E-5 76-77 4.3533017835042076E-5 8.706603567008415E-5 0.0 1.5440073100643548 4.3533017835042076E-5 78-79 4.3533017835042076E-5 8.706603567008415E-5 0.0 1.724821699642202 4.3533017835042076E-5 80-81 4.3533017835042076E-5 8.706603567008415E-5 0.0 1.9248123835763855 4.3533017835042076E-5 82-83 4.3533017835042076E-5 8.706603567008415E-5 0.0 2.1411279491987094 4.3533017835042076E-5 84-85 4.3533017835042076E-5 8.706603567008415E-5 0.0 2.363059274121754 4.3533017835042076E-5 86-87 4.3533017835042076E-5 8.706603567008415E-5 0.0 2.6010542826259293 4.3533017835042076E-5 88 4.3533017835042076E-5 8.706603567008415E-5 0.0 2.797518792115474 4.3533017835042076E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 220 0.0 51.272385 2 ATAACGC 265 0.0 42.56575 3 TACCTGG 2400 0.0 41.124725 2 GGTATCA 3250 0.0 40.92677 1 GTACCTG 2695 0.0 37.670326 1 GTATCAA 5320 0.0 33.836975 1 TAACGCA 330 0.0 32.757355 4 ACCTGGG 3020 0.0 32.059387 3 TCTGTCG 740 0.0 31.121414 8 TATCACG 345 0.0 28.608503 2 TCAACGC 6125 0.0 27.93124 4 CAACGCA 6215 0.0 27.526766 5 ATCAACG 6235 0.0 27.438469 3 AACGCAG 6550 0.0 26.40593 6 CTGTCGC 905 0.0 25.447342 9 GTATAAC 670 0.0 25.254112 1 TATCAAC 7000 0.0 24.842691 2 GTACATG 8800 0.0 23.874222 1 TACATGG 8780 0.0 23.23219 2 ACGCAGA 7390 0.0 23.086449 7 >>END_MODULE