FastQCFastQC Report
Wed 25 May 2016
SRR2431377_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2431377_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297107
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150810.6565214419702696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108330.4715931822070108No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86550.37677826936228914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77170.3359442986330197No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49990.21762155615737533No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37500.16324881688140777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23860.1038697805544104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53600.057.1805231
TACCTGG23550.048.696542
ATAACGC2950.047.7967533
GTATCAA109850.045.5309681
TATAACG2800.043.642992
GTACCTG27750.042.3489231
TAACGCA3800.037.104574
ACCTGGG31550.036.3487633
ATCAACG141650.035.3040243
TCAACGC142400.035.2823454
TATCAAC143000.034.9378662
CAACGCA144200.034.8411645
AACGCAG149400.033.7543226
ACGCAGA167100.030.122667
CGCAGAG167500.029.9946048
ACAACGC3150.029.8413583
TACAACG2850.029.68432
TCTGTCG7100.029.125588
GCAGAGT178600.027.8146619
GTACATG81050.023.9531361
Produced by FastQC (version 0.11.5)