##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2431377_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2297107 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.76449508011599 34.0 31.0 34.0 31.0 34.0 2 32.88248914830698 34.0 31.0 34.0 31.0 34.0 3 32.95333957016369 34.0 31.0 34.0 31.0 34.0 4 36.34150694765198 37.0 37.0 37.0 35.0 37.0 5 36.30174475982181 37.0 37.0 37.0 35.0 37.0 6 36.33559777581105 37.0 37.0 37.0 35.0 37.0 7 36.198882333300105 37.0 37.0 37.0 35.0 37.0 8 36.25825701632532 37.0 37.0 37.0 35.0 37.0 9 38.0774983490103 39.0 39.0 39.0 35.0 39.0 10-11 38.070471249271364 39.0 39.0 39.0 35.0 39.0 12-13 38.051368961045355 39.0 39.0 39.0 35.0 39.0 14-15 39.5403910222728 41.0 39.5 41.0 37.0 41.0 16-17 39.54852451366001 41.0 39.5 41.0 37.0 41.0 18-19 39.62153352020607 41.0 40.0 41.0 37.0 41.0 20-21 39.605956753429425 41.0 40.0 41.0 37.0 41.0 22-23 39.49625681346146 41.0 40.0 41.0 36.0 41.0 24-25 39.457784508949736 41.0 39.5 41.0 36.0 41.0 26-27 39.3545224493243 41.0 39.0 41.0 36.0 41.0 28-29 39.252265784745774 41.0 39.0 41.0 35.0 41.0 30-31 39.065028098386364 41.0 39.0 41.0 35.0 41.0 32-33 38.809569602112575 41.0 38.0 41.0 35.0 41.0 34-35 38.663951222124176 41.0 38.0 41.0 35.0 41.0 36-37 38.51962359611459 41.0 38.0 41.0 35.0 41.0 38-39 38.384069832184565 41.0 38.0 41.0 35.0 41.0 40-41 38.278681619968076 41.0 38.0 41.0 35.0 41.0 42-43 38.13942319622029 41.0 38.0 41.0 34.0 41.0 44-45 37.928636759193196 41.0 38.0 41.0 33.5 41.0 46-47 37.60332627082674 40.0 38.0 41.0 33.0 41.0 48-49 37.319793113686046 40.0 38.0 41.0 33.0 41.0 50-51 37.17477788365975 40.0 38.0 41.0 32.0 41.0 52-53 37.06009363081476 40.0 37.0 41.0 32.0 41.0 54-55 36.92388817760775 40.0 37.0 41.0 31.5 41.0 56-57 36.77766425334127 40.0 37.0 41.0 31.0 41.0 58-59 36.60992979430213 40.0 36.0 41.0 31.0 41.0 60-61 36.41844567971801 40.0 36.0 41.0 31.0 41.0 62-63 36.18143168777075 39.0 35.0 41.0 30.5 41.0 64-65 35.90959824683831 39.0 35.0 41.0 30.0 41.0 66-67 35.634542274260625 39.0 35.0 41.0 30.0 41.0 68-69 35.36936916739185 38.0 35.0 40.5 29.5 41.0 70-71 35.10976654548526 37.0 35.0 40.0 29.0 41.0 72-73 34.85067891917964 37.0 35.0 39.5 29.0 41.0 74-75 34.555934921620974 36.0 35.0 39.0 29.0 41.0 76-77 34.23442508337661 36.0 35.0 39.0 29.0 41.0 78-79 33.91190985008535 35.5 35.0 38.5 29.0 40.0 80-81 33.59630961901209 35.0 35.0 37.0 29.0 39.0 82-83 33.275366145329755 35.0 34.0 37.0 29.0 39.0 84-85 33.00129358362497 35.0 34.0 36.5 29.0 39.0 86-87 32.7652991349554 35.0 34.0 36.0 28.5 37.0 88-89 32.53454627929827 35.0 34.0 36.0 27.5 37.0 90-91 32.32396422978991 35.0 34.0 35.5 27.0 37.0 92-93 32.160150136671916 35.0 34.0 35.0 27.0 36.0 94-95 32.01327909409531 35.0 34.0 35.0 27.0 36.0 96-97 31.864302359446036 35.0 34.0 35.0 26.0 36.0 98-99 31.738106453029836 35.0 34.0 35.0 26.0 36.0 100 31.628722998101527 35.0 34.0 35.0 25.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19100955570818456 1101 2 0.2107348096425028 1101 3 0.2014271555525582 1101 4 0.1379326997127066 1101 5 0.16956214358425825 1101 6 0.18191989039600287 1101 7 0.2156352344438517 1101 8 0.24805651838831722 1101 9 0.32238003795426096 1101 10-11 0.2993171223907325 1101 12-13 0.23481828978289343 1101 14-15 0.30020445854199806 1101 16-17 0.32941363859332284 1101 18-19 0.32280559885851545 1101 20-21 0.32116574159929456 1101 22-23 0.33688811304423183 1101 24-25 0.3341592810486631 1101 26-27 0.29297469887937666 1101 28-29 0.3166223585295853 1101 30-31 0.3765766759354605 1101 32-33 0.32353153316046246 1101 34-35 0.3689604913902116 1101 36-37 0.3727678359597846 1101 38-39 0.3467950537247546 1101 40-41 0.4263206261393364 1101 42-43 0.43164827485191637 1101 44-45 0.47095134638264824 1101 46-47 0.4042453550717653 1101 48-49 0.3988470508149291 1101 50-51 0.3911438563800118 1101 52-53 0.44799475578075487 1101 54-55 0.4208856624481925 1101 56-57 0.42635889007056704 1101 58-59 0.35592619046841634 1101 60-61 0.29227905618693484 1101 62-63 0.3527204923463785 1101 64-65 0.38823754409630595 1101 66-67 0.3610920074650181 1101 68-69 0.3105486681558247 1101 70-71 0.3353349712111964 1101 72-73 0.2988022552657412 1101 74-75 0.319454791750573 1101 76-77 0.306145427813064 1101 78-79 0.2775277677756307 1101 80-81 0.2554195804708215 1101 82-83 0.28491672014994407 1101 84-85 0.20992049607877306 1101 86-87 0.21598289348388278 1101 88-89 0.26927827278413474 1101 90-91 0.2860798821576864 1101 92-93 0.28693652612038534 1101 94-95 0.3018846407889981 1101 96-97 0.37087687257691826 1101 98-99 0.33805411122563456 1101 100 0.3848550342571251 1102 1 0.07782191077824052 1102 2 0.0826390602318341 1102 3 0.1369669687161803 1102 4 0.08728408427109713 1102 5 0.09063692396430412 1102 6 0.06808777754293516 1102 7 0.09764536586995831 1102 8 0.1257625110884959 1102 9 0.15190860909444126 1102 10-11 0.11940604671770672 1102 12-13 0.15835785380837564 1102 14-15 0.2754952943057205 1102 16-17 0.23120245805073836 1102 18-19 0.237546156987257 1102 20-21 0.2005812103411202 1102 22-23 0.161717024319195 1102 24-25 0.21252691536439983 1102 26-27 0.1957114200512251 1102 28-29 0.12206523265751912 1102 30-31 0.12307489307656283 1102 32-33 0.1889241797393595 1102 34-35 0.24105752744051756 1102 36-37 0.2538599163234849 1102 38-39 0.2762566713832513 1102 40-41 0.26222489471560806 1102 42-43 0.33561667028273234 1102 44-45 0.29116823624512733 1102 46-47 0.26775793678672954 1102 48-49 0.3450492872602169 1102 50-51 0.37803167045407093 1102 52-53 0.3906230942102269 1102 54-55 0.38631731579256723 1102 56-57 0.3925465685824818 1102 58-59 0.42846198326585494 1102 60-61 0.3671515714191358 1102 62-63 0.3786114362421742 1102 64-65 0.4196671712319997 1102 66-67 0.4388135378122726 1102 68-69 0.40676860929072944 1102 70-71 0.42778722428005267 1102 72-73 0.45412945333333 1102 74-75 0.464471077099077 1102 76-77 0.4469198111316004 1102 78-79 0.41497514284611015 1102 80-81 0.36880646893548175 1102 82-83 0.3513047137584593 1102 84-85 0.37886327496207883 1102 86-87 0.3198961226135566 1102 88-89 0.29199260979397934 1102 90-91 0.36301467560887346 1102 92-93 0.3984562658686315 1102 94-95 0.3733036173651314 1102 96-97 0.4324716838447351 1102 98-99 0.4536788418402473 1102 100 0.4757348659606784 1103 1 0.14772142871852623 1103 2 0.1779092535308564 1103 3 0.138647394206437 1103 4 0.12913682087544487 1103 5 0.1344291194550422 1103 6 0.1088459907143502 1103 7 0.0848992054318316 1103 8 0.08234240776899071 1103 9 0.14270944113069817 1103 10-11 0.1738106925651408 1103 12-13 0.20051904528185105 1103 14-15 0.2965861941333543 1103 16-17 0.27654928325539174 1103 18-19 0.2287013352604319 1103 20-21 0.2608693966818194 1103 22-23 0.30321466096146565 1103 24-25 0.2686839245218735 1103 26-27 0.3164066237068113 1103 28-29 0.25842775881607594 1103 30-31 0.24541922831672736 1103 32-33 0.3136552000332671 1103 34-35 0.20887436917178093 1103 36-37 0.149392463225702 1103 38-39 0.11146964263082992 1103 40-41 0.12207074933213136 1103 42-43 0.1601960105423217 1103 44-45 0.11766905093615776 1103 46-47 0.1583910792844776 1103 48-49 0.07943930511491715 1103 50-51 0.09295711749420121 1103 52-53 0.1029941284947995 1103 54-55 0.13088534254198692 1103 56-57 0.10280411933442934 1103 58-59 0.13733620981502526 1103 60-61 0.08818872778008569 1103 62-63 0.0780002223763745 1103 64-65 0.21010996473210497 1103 66-67 0.178868038820454 1103 68-69 0.17802742763666402 1103 70-71 0.21976418242464746 1103 72-73 0.2117839140410709 1103 74-75 0.13754822401843114 1103 76-77 0.13710756884120912 1103 78-79 0.03197017608348318 1103 80-81 0.08039677751820307 1103 82-83 0.06234766521645696 1103 84-85 0.09992123032132127 1103 86-87 0.011802278296542568 1103 88-89 -0.015156883674080746 1103 90-91 0.0041968448757003785 1103 92-93 0.09014627416342336 1103 94-95 0.051059918746055644 1103 96-97 0.08788413656756333 1103 98-99 0.14570564125413554 1103 100 0.22585799030605713 1104 1 0.06290704199402342 1104 2 0.08443986470805243 1104 3 0.11247535994741753 1104 4 0.03964973078800682 1104 5 0.10877409456124099 1104 6 0.06584004183680037 1104 7 0.05013401587605415 1104 8 0.08725128743753885 1104 9 0.1561369236071286 1104 10-11 0.11773993384503001 1104 12-13 0.18245791074619433 1104 14-15 0.1508365455016687 1104 16-17 0.21466641997642455 1104 18-19 0.22310980517055867 1104 20-21 0.22922223885066018 1104 22-23 0.24085682919447748 1104 24-25 0.2318194004077867 1104 26-27 0.22522070848438602 1104 28-29 0.18468834050487004 1104 30-31 0.14837673862982115 1104 32-33 0.16552248763434818 1104 34-35 0.22929399691756913 1104 36-37 0.28551633919277464 1104 38-39 0.26233529231933517 1104 40-41 0.31674925246763763 1104 42-43 0.3038870575544905 1104 44-45 0.26287562485878624 1104 46-47 0.3222945392299863 1104 48-49 0.3330159560975332 1104 50-51 0.3088488867125747 1104 52-53 0.3665643631053115 1104 54-55 0.34580213710634666 1104 56-57 0.364103959728574 1104 58-59 0.3463387872010699 1104 60-61 0.35699557405285276 1104 62-63 0.3737335965173898 1104 64-65 0.3901598308179004 1104 66-67 0.3578167141964954 1104 68-69 0.33963695047457776 1104 70-71 0.2997724560692774 1104 72-73 0.3368660805999255 1104 74-75 0.30336781372189137 1104 76-77 0.27267715919863633 1104 78-79 0.28047305373480924 1104 80-81 0.33270911918759083 1104 82-83 0.2996248812658351 1104 84-85 0.25984612849831734 1104 86-87 0.26074368900900424 1104 88-89 0.2603750081616454 1104 90-91 0.33743871594511177 1104 92-93 0.2559981886302296 1104 94-95 0.30283180158317435 1104 96-97 0.32485211860919705 1104 98-99 0.348815862718002 1104 100 0.3525607859683362 1105 1 0.025545692929753727 1105 2 0.06464247632096942 1105 3 0.0883371024359576 1105 4 -0.03002427401455776 1105 5 -0.030023385109323897 1105 6 -0.055235428480600035 1105 7 -0.35765627503158015 1105 8 -0.3114808151530255 1105 9 -0.30076124099132784 1105 10-11 -0.2826930780346544 1105 12-13 -0.17892331020435392 1105 14-15 -0.09876220323564411 1105 16-17 -0.0753858115275392 1105 18-19 -0.1105420773438297 1105 20-21 -0.1310023788714645 1105 22-23 -0.12484385452635394 1105 24-25 -0.17652443807023843 1105 26-27 -0.2136627360865475 1105 28-29 -0.2030219415610972 1105 30-31 -0.1682921724034827 1105 32-33 -0.2147426267604544 1105 34-35 -0.20503149735232284 1105 36-37 -0.10519809363125177 1105 38-39 -0.07496981555737392 1105 40-41 -0.140891439144319 1105 42-43 -0.023406477688851623 1105 44-45 -0.05858340583132815 1105 46-47 -0.03683920314387734 1105 48-49 0.009752944994893653 1105 50-51 -0.06394031784616772 1105 52-53 -0.08315833502322789 1105 54-55 -0.07173143975020224 1105 56-57 -0.0954898966666704 1105 58-59 -0.11114734487632205 1105 60-61 -0.1443847114305825 1105 62-63 -0.10266820848148228 1105 64-65 -0.03145613579497564 1105 66-67 -0.047493551574234516 1105 68-69 -0.029084763904684507 1105 70-71 -0.019189480981331997 1105 72-73 -0.030093428762661745 1105 74-75 -0.0022688093625617967 1105 76-77 -0.05501300655254937 1105 78-79 -0.04791220556686682 1105 80-81 -0.04502212413117945 1105 82-83 -0.028698799404928366 1105 84-85 -0.007590619968915746 1105 86-87 0.022856762086341575 1105 88-89 -0.027643431102021054 1105 90-91 -0.01069913871197059 1105 92-93 -0.025650304324720707 1105 94-95 -0.0023368993472381305 1105 96-97 -0.03274828756458348 1105 98-99 -0.046894454805180175 1105 100 -0.05074454370460302 1106 1 0.034047816456634905 1106 2 0.01476453324738003 1106 3 0.04451337529624766 1106 4 0.005594099649933071 1106 5 0.02674706913411029 1106 6 0.05730419281516674 1106 7 0.027055952428945318 1106 8 0.07894681873462162 1106 9 0.020367379434077293 1106 10-11 0.08574253230520412 1106 12-13 0.04050158114324631 1106 14-15 0.1294556142254848 1106 16-17 0.11878450063679225 1106 18-19 0.10441723523270241 1106 20-21 0.11405442248889841 1106 22-23 0.07558423538250025 1106 24-25 0.04763127250877375 1106 26-27 0.046083826564526476 1106 28-29 0.0895517797892822 1106 30-31 0.041876290024816853 1106 32-33 -0.012438704301160897 1106 34-35 -0.06682082125456645 1106 36-37 0.0029430606082954114 1106 38-39 -0.002488361904269709 1106 40-41 0.015102282726211058 1106 42-43 0.09527286351725905 1106 44-45 0.11361665957922185 1106 46-47 0.02988142009460404 1106 48-49 0.017748947327724807 1106 50-51 0.03437932416293421 1106 52-53 0.06453905179712649 1106 54-55 -0.06118554851909863 1106 56-57 -0.004553478492368868 1106 58-59 0.0478736185611055 1106 60-61 -0.057802805924588085 1106 62-63 0.0036903214486514457 1106 64-65 0.029907752384339403 1106 66-67 -0.0038995718379624122 1106 68-69 -0.02756065552411968 1106 70-71 -0.07514306051623976 1106 72-73 -0.10061786562101105 1106 74-75 -0.06996716166531769 1106 76-77 -0.04623067936945091 1106 78-79 -0.07270982834256046 1106 80-81 -0.07157458242394199 1106 82-83 -0.05217409427785924 1106 84-85 -0.00843857893556077 1106 86-87 -0.03713260513791994 1106 88-89 -0.020533953591879595 1106 90-91 -0.03532900446715814 1106 92-93 -0.0623288827641062 1106 94-95 -0.05692624512425226 1106 96-97 -0.08219051662474897 1106 98-99 -0.05604667575315858 1106 100 -0.13393679547676385 1107 1 -0.06725655429892186 1107 2 -0.058937640566213645 1107 3 -0.028564046321413628 1107 4 -0.10800016184309413 1107 5 -0.0767939716684154 1107 6 -0.12950306771714537 1107 7 -0.1002401270564377 1107 8 -0.07820512455764117 1107 9 -0.016210169897995286 1107 10-11 -0.022664324179388018 1107 12-13 0.015639962245586503 1107 14-15 -0.006399435322627767 1107 16-17 -0.0034184108704664595 1107 18-19 0.008383784224207602 1107 20-21 0.015288534435349277 1107 22-23 0.00983534128761221 1107 24-25 0.06698342052532524 1107 26-27 0.008253715001870887 1107 28-29 0.005237475902696076 1107 30-31 0.07656083861475338 1107 32-33 0.0648818061244043 1107 34-35 0.05664080787295944 1107 36-37 0.09788919181483635 1107 38-39 0.1031926894712285 1107 40-41 0.06500156466625384 1107 42-43 0.08824745915184451 1107 44-45 0.04533293630393587 1107 46-47 0.07482747981919857 1107 48-49 0.09615157525817608 1107 50-51 0.15861955051607168 1107 52-53 0.18338560801942094 1107 54-55 0.15986263571243597 1107 56-57 0.11514291862700787 1107 58-59 0.023589295790579 1107 60-61 0.03033983264236184 1107 62-63 0.06711887190919441 1107 64-65 0.1210400438603969 1107 66-67 0.1172114926775123 1107 68-69 0.10038737900833183 1107 70-71 0.13632979953672475 1107 72-73 0.11540478049443692 1107 74-75 0.025561388541063934 1107 76-77 0.12076841805185978 1107 78-79 0.08806080897139879 1107 80-81 0.07827897489885771 1107 82-83 0.10405573192888085 1107 84-85 0.10103375564569461 1107 86-87 0.12356579569978976 1107 88-89 0.08372432910046967 1107 90-91 0.15275067439146994 1107 92-93 0.15934028100515718 1107 94-95 0.04170287414194007 1107 96-97 0.07194067911251167 1107 98-99 0.10394472894012452 1107 100 0.05312517905151992 1108 1 -0.051867640338336685 1108 2 -0.06019723360686413 1108 3 -0.026659774769179023 1108 4 -0.03407648043427258 1108 5 -0.15115663286142222 1108 6 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-0.18270533752534845 2214 50-51 -0.1361114776986696 2214 52-53 -0.10753952373703157 2214 54-55 -0.4034344407806145 2214 56-57 -0.19941806231546622 2214 58-59 -0.14442489732264363 2214 60-61 -0.1373989866369456 2214 62-63 -0.15596758718628934 2214 64-65 -0.14525299133944003 2214 66-67 -0.09150186702616736 2214 68-69 -0.048785606346982036 2214 70-71 -0.09984724140208812 2214 72-73 -0.05967445914796343 2214 74-75 -0.09913801526815291 2214 76-77 -0.13064499852715272 2214 78-79 -0.11992928357250321 2214 80-81 -0.13248797052367678 2214 82-83 -0.0382116507764394 2214 84-85 -0.08252651935659827 2214 86-87 -0.03396518564281337 2214 88-89 -0.041304072845292694 2214 90-91 -0.17124032415664203 2214 92-93 -0.17425575544079663 2214 94-95 -0.12817541548216482 2214 96-97 -0.06622603423575768 2214 98-99 -0.11238324601186278 2214 100 -0.07333335910926309 2215 1 -0.11626640435618185 2215 2 -0.10316560542450048 2215 3 -0.10256472625270874 2215 4 -0.0518918514723552 2215 5 -0.049788509851929064 2215 6 -0.11163138685542151 2215 7 -0.10082220639367989 2215 8 -0.09402023692523187 2215 9 -0.12789391685620188 2215 10-11 -0.15719035168081064 2215 12-13 -0.1987086568676375 2215 14-15 -0.2143720211192246 2215 16-17 -0.22641518567006358 2215 18-19 -0.21085267977522193 2215 20-21 -0.18317368017243751 2215 22-23 -0.17653265139418295 2215 24-25 -0.17987469302319425 2215 26-27 -0.17328206861894557 2215 28-29 -0.2278061783701375 2215 30-31 -0.18485988229134875 2215 32-33 -0.21973038701186454 2215 34-35 -0.21851497243766005 2215 36-37 -0.2182318896337776 2215 38-39 -0.24500873570053727 2215 40-41 -0.1936924416499508 2215 42-43 -0.13892004550032766 2215 44-45 -0.18614704374545 2215 46-47 -0.24175686073691338 2215 48-49 -0.13870793170217866 2215 50-51 -0.17017531600267688 2215 52-53 -0.1960660967293677 2215 54-55 -0.19468714465187276 2215 56-57 -0.24221325577537556 2215 58-59 -0.2378096181086491 2215 60-61 -0.17282989967942086 2215 62-63 -0.2567034378889801 2215 64-65 -0.22882067087264346 2215 66-67 -0.32652642959561007 2215 68-69 -0.2070693312872649 2215 70-71 -0.27824796089484494 2215 72-73 -0.2300444885794164 2215 74-75 -0.2615323301463093 2215 76-77 -0.20468186868232152 2215 78-79 -0.19972709943470335 2215 80-81 -0.21526514459011992 2215 82-83 -0.15777484887558302 2215 84-85 -0.11484470887263143 2215 86-87 -0.13484835402824302 2215 88-89 -0.1294791425750219 2215 90-91 -0.1294004023155253 2215 92-93 -0.1698840158027437 2215 94-95 -0.2285770830939562 2215 96-97 -0.22658997594760777 2215 98-99 -0.25436759884043525 2215 100 -0.1726338166183936 2216 1 -0.15721090597202192 2216 2 -0.22041052675126593 2216 3 -0.2781586832515828 2216 4 -0.14505767577149697 2216 5 -0.17434689642117718 2216 6 -0.15610594526574317 2216 7 -0.2503619211954984 2216 8 -0.23983438774940424 2216 9 -0.2336486933603723 2216 10-11 -0.25956087033785025 2216 12-13 -0.27968337301400936 2216 14-15 -0.3894244731071197 2216 16-17 -0.48478083826496743 2216 18-19 -0.4634262835973786 2216 20-21 -0.4012546975889748 2216 22-23 -0.42287657204725093 2216 24-25 -0.3949573001530595 2216 26-27 -0.44841192767323435 2216 28-29 -0.4460222454022542 2216 30-31 -0.44254612960817497 2216 32-33 -0.46674326055411086 2216 34-35 -0.39732600844994437 2216 36-37 -0.531545988196001 2216 38-39 -0.5260629612687922 2216 40-41 -0.5158481922217462 2216 42-43 -0.5198482128829767 2216 44-45 -0.5621837306802462 2216 46-47 -0.5515398928989157 2216 48-49 -0.5970909138827523 2216 50-51 -0.5702351559290904 2216 52-53 -0.6207317509396333 2216 54-55 -0.637016267097799 2216 56-57 -0.602169061174699 2216 58-59 -0.6936088410210388 2216 60-61 -0.5719481931653903 2216 62-63 -0.6757379269714576 2216 64-65 -0.6820751528094888 2216 66-67 -0.5635926079456723 2216 68-69 -0.5566484088354358 2216 70-71 -0.631979606262405 2216 72-73 -0.6348917007847703 2216 74-75 -0.5484767737989102 2216 76-77 -0.5420435289397716 2216 78-79 -0.526314484731607 2216 80-81 -0.5474276322016607 2216 82-83 -0.5263624270665801 2216 84-85 -0.49221556010567724 2216 86-87 -0.44526654698124446 2216 88-89 -0.3732827763955413 2216 90-91 -0.4292020563585517 2216 92-93 -0.5588881977085194 2216 94-95 -0.546245433070343 2216 96-97 -0.6221688292445293 2216 98-99 -0.5538161878154462 2216 100 -0.5448743859505143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22.0 3 197.0 4 115.0 5 207.0 6 376.0 7 573.0 8 1069.0 9 1714.0 10 2408.0 11 3585.0 12 5830.0 13 9143.0 14 8352.0 15 9323.0 16 11353.0 17 17525.0 18 22197.0 19 11576.0 20 6869.0 21 5726.0 22 5668.0 23 5779.0 24 6803.0 25 8059.0 26 9666.0 27 12473.0 28 15412.0 29 18841.0 30 23313.0 31 28552.0 32 36470.0 33 46950.0 34 65776.0 35 109003.0 36 168121.0 37 381232.0 38 753893.0 39 466023.0 40 16913.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.097957536365584 21.668974867244 14.044468090015904 23.18859950637451 2 14.030567157109857 22.469620470278688 40.841955051268855 22.657857321342593 3 14.541155041979126 29.789507960928024 32.207780787377445 23.461556209715408 4 10.152747890449197 18.959249090029704 40.4785610083877 30.409442011133397 5 10.957217056062255 37.2698354930789 35.27976711576779 16.49318033509105 6 26.467247716366714 40.61856935702168 19.262881528809935 13.651301397801669 7 23.055086245438282 33.9799582692491 25.646258533015658 17.318696952296957 8 23.32573101731874 34.16575718936906 23.676476542015674 18.83203525129652 9 24.37317895944769 15.408555195730978 24.11176318734826 36.10650265747307 10-11 22.03473760691165 26.996848644838924 30.58753902190886 20.38087472634057 12-13 23.679980949951396 25.88466710518927 30.88802567751524 19.547326267344097 14-15 21.52786091374934 26.264928015978363 29.471722475269978 22.735488595002323 16-17 19.39119509887872 30.119014917459218 29.20556160422653 21.284228379435525 18-19 18.584049415199207 27.425148240808984 33.73630396842637 20.25449837556544 20-21 21.219821279548583 27.00662180734289 32.402800566103366 19.37075634700517 22-23 19.823756423846536 27.86146040342083 33.14402258494932 19.17076058778332 24-25 19.870027737080427 26.698164276738705 33.1575323815123 20.27427560466857 26-27 19.873531596749025 27.044783292640638 33.119280413025926 19.962404697584407 28-29 19.467486313641214 26.934815036351484 33.42808379222985 20.16961485777745 30-31 20.76977762066915 27.798809437778456 32.0050664630446 19.4263464785078 32-33 18.985335030906555 27.4855142861061 33.69952039710849 19.82963028587885 34-35 19.494089210818004 26.17726011390579 33.21983677741179 21.10881389786442 36-37 20.701326981907403 27.822932960443914 31.86870188596776 19.607038171680923 38-39 19.930906972775492 27.38331980872842 32.606284239597954 20.079488978898134 40-41 20.15904039417917 27.198597839424142 32.110077963837156 20.532283802559526 42-43 19.271784263073258 27.770173967630406 32.6632257974641 20.294815971832236 44-45 20.328667151917028 27.077209659500518 31.920738184211718 20.673385004370736 46-47 20.348624851710365 28.66035415374234 30.855345501246177 20.135675493301118 48-49 19.827348458645627 27.832883373032725 31.49938323187369 20.840384936447958 50-51 20.61109228288828 27.46530546233953 30.566368179476278 21.357234075295914 52-53 19.95227474215932 28.287325816195285 30.851529957361578 20.908869484283816 54-55 20.663321999425797 28.285358493868003 30.186220432421113 20.86509907428509 56-57 20.20011858492143 28.343276356848325 30.294464121531263 21.162140936698986 58-59 19.53028852579197 27.34338778916464 31.365583688238736 21.760739996804652 60-61 21.152449743110655 28.175659027172546 29.753040575543576 20.91885065417322 62-63 20.194226912372823 28.26276703697303 30.776668217893207 20.766337832760946 64-65 20.332596609561506 27.16172994988914 30.94607695679827 21.559596483751083 66-67 20.51876561080063 28.07949196536919 30.2111478121752 21.190594611654976 68-69 20.795336881736652 27.958205372494195 30.365360165548623 20.881097580220537 70-71 21.58909006849181 27.53111255546757 29.871122385411287 21.008674990629338 72-73 20.422597138572247 28.11223257099697 31.01974006412419 20.44543022630659 74-75 20.58975066122695 27.215406854557934 30.58027984086584 21.61456264334928 76-77 20.860455422549542 28.26486468249606 29.908373501777785 20.966306393176605 78-79 20.62204297985787 28.21312834229255 30.312231724157147 20.852596953692434 80-81 20.9903122807682 27.7213692928446 29.943426087906687 21.344892338480513 82-83 20.005289323837218 28.140704720775943 30.855695056332387 20.998310899054452 84-85 20.540569249157137 27.429493593710447 30.250794650683467 21.779142506448952 86-87 20.81948615165744 28.239979313028872 29.94160458108448 20.998929954229204 88-89 19.84657421416791 27.812194788015958 30.526193895139965 21.81503710267617 90-91 20.377381325268555 27.629435194845254 29.886008959688372 22.107174520197827 92-93 20.029093951740524 28.37796539112711 30.38163010586228 21.211310551270092 94-95 20.224641327874817 27.310189788444827 30.327166964725716 22.13800191895464 96-97 20.044195739412306 28.090589076268994 30.276932975889988 21.58828220842871 98-99 19.554760722089178 27.683575113721602 30.59810879747433 22.16355536671489 100 20.528220534924834 27.624156901533414 29.766529257524816 22.081093306016935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4132.0 1 2488.5 2 4103.0 3 5796.5 4 2622.0 5 1122.0 6 1966.0 7 4346.5 8 7383.5 9 7791.5 10 4745.0 11 2359.5 12 2033.5 13 2156.0 14 2427.5 15 2667.0 16 2947.5 17 3381.0 18 3789.5 19 4258.0 20 5141.0 21 6222.0 22 7564.0 23 9853.0 24 12819.5 25 16069.5 26 20534.0 27 26479.0 28 33563.5 29 41549.5 30 48704.0 31 55214.0 32 61255.5 33 66169.0 34 70724.5 35 75126.0 36 78046.0 37 80359.0 38 83577.0 39 86285.5 40 88373.5 41 87775.5 42 84009.0 43 80874.5 44 77561.0 45 72571.0 46 67814.0 47 64431.0 48 61924.5 49 60825.5 50 60862.0 51 58408.5 52 53309.5 53 49476.5 54 47131.5 55 45018.5 56 42139.0 57 38646.0 58 36228.5 59 33553.0 60 30112.0 61 25695.0 62 20622.0 63 15625.0 64 11310.5 65 8210.0 66 5789.5 67 4077.0 68 2819.5 69 1921.0 70 1292.0 71 928.0 72 684.0 73 418.0 74 283.0 75 217.0 76 152.0 77 112.0 78 74.0 79 44.0 80 23.0 81 8.0 82 3.5 83 3.0 84 3.0 85 4.0 86 2.0 87 2.0 88 2.0 89 1.5 90 3.5 91 3.5 92 2.0 93 0.5 94 2.0 95 4.5 96 5.5 97 6.0 98 3.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009751395995049426 2 4.353301783504208E-5 3 5.65929231855547E-4 4 0.002350782963092272 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.706603567008416E-5 24-25 6.529952675256312E-5 26-27 5.006297051029839E-4 28-29 8.924268656183625E-4 30-31 0.001784853731236725 32-33 0.004462134328091813 34-35 0.005267495158040091 36-37 0.003917971605153787 38-39 0.0025684480522674825 40-41 0.0035044079357208872 42-43 5.65929231855547E-4 44-45 4.788631961854629E-4 46-47 0.00356970746247345 48-49 0.002285483436339709 50-51 0.0010230259191234888 52-53 2.176650891752104E-5 54-55 7.618278121132363E-4 56-57 8.271273388657994E-4 58-59 6.965282853606733E-4 60-61 3.917971605153787E-4 62-63 0.0 64-65 0.0 66-67 8.488938477833205E-4 68-69 7.618278121132363E-4 70-71 0.0019154527847418516 72-73 8.706603567008416E-5 74-75 0.002241950418504667 76-77 4.135636694328997E-4 78-79 0.0013495235528863043 80-81 0.0010230259191234888 82-83 0.001175391481546136 84-85 0.0012406910082986991 86-87 3.917971605153787E-4 88-89 0.0015454221331439935 90-91 0.001763087222319204 92-93 0.002873179177112777 94-95 0.0010012594102059677 96-97 0.00230724994525723 98-99 0.0021766508917521036 100 0.003221443319793114 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2297107.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.22018541533633 #Duplication Level Percentage of deduplicated Percentage of total 1 83.09126394892952 58.34683960888483 2 11.918999255326224 16.73908675348526 3 2.5715049903636147 5.41714671659387 4 0.8787215464343174 2.468159596762754 5 0.4015555033324787 1.409865094927768 6 0.2509414488918918 1.0572693041749073 7 0.15975252133336698 0.7852496168017552 8 0.1190504824011094 0.6687797558392908 9 0.08644649919976233 0.546326028208263 >10 0.46909005065911924 6.028663717411943 >50 0.03484653844012576 1.687943949833421 >100 0.016462835391015174 2.0195401362027807 >500 8.682413710934374E-4 0.42189904483894713 >1k 2.48068963169305E-4 0.5617336683911537 >5k 1.240344815846525E-4 0.7129780307698246 >10k+ 1.240344815846525E-4 1.1285189768732737 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15081 0.6565214419702696 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10833 0.4715931822070108 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8655 0.37677826936228914 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7717 0.3359442986330197 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4999 0.21762155615737533 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3750 0.16324881688140777 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2386 0.1038697805544104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.1766508917521038E-5 0.0 14-15 0.0 0.0 0.0 4.3533017835042076E-5 0.0 16-17 0.0 0.0 0.0 4.3533017835042076E-5 0.0 18-19 0.0 0.0 0.0 8.706603567008415E-5 0.0 20-21 0.0 0.0 0.0 1.741320713401683E-4 0.0 22-23 0.0 0.0 0.0 4.3533017835042074E-4 0.0 24-25 0.0 0.0 0.0 0.0010012594102059677 0.0 26-27 0.0 0.0 0.0 0.0018719197669068094 0.0 28-29 0.0 0.0 0.0 0.0036132404803084924 0.0 30-31 0.0 0.0 0.0 0.006899983326854169 0.0 32-33 0.0 0.0 0.0 0.013016372332677582 0.0 34-35 0.0 0.0 0.0 0.023224865014994947 0.0 36-37 0.0 0.0 0.0 0.03922324906937291 0.0 38-39 0.0 0.0 0.0 0.06214338295952257 0.0 40-41 0.0 0.0 0.0 0.09037454502554734 0.0 42-43 0.0 0.0 0.0 0.11978109857311828 0.0 44-45 0.0 0.0 0.0 0.15388921804687375 0.0 46-47 0.0 0.0 0.0 0.19361309682134964 0.0 48-49 0.0 0.0 0.0 0.23649311938886608 0.0 50-51 0.0 0.0 0.0 0.28407470788256706 0.0 52-53 0.0 0.0 0.0 0.33200456051894844 0.0 54-55 0.0 0.0 0.0 0.3837217857069784 0.0 56-57 0.0 0.0 0.0 0.44473331020278983 0.0 58-59 0.0 0.0 0.0 0.511077629383394 0.0 60-61 0.0 0.0 0.0 0.585649688934821 0.0 62-63 0.0 0.0 0.0 0.6612447744053717 0.0 64-65 0.0 0.0 0.0 0.7518587510290118 0.0 66-67 0.0 0.0 0.0 0.856729790993628 0.0 68-69 0.0 0.0 0.0 0.9691973425704592 0.0 70-71 0.0 0.0 0.0 1.1003840918163585 0.0 72-73 0.0 0.0 0.0 1.2485269515090067 0.0 74-75 0.0 0.0 0.0 1.3986940965309844 0.0 76-77 0.0 0.0 0.0 1.5579596422804858 0.0 78-79 0.0 0.0 0.0 1.7414730789641055 0.0 80-81 0.0 0.0 0.0 1.9445763736734945 0.0 82-83 0.0 0.0 0.0 2.164418113740457 0.0 84-85 0.0 0.0 0.0 2.3895491154743773 0.0 86-87 0.0 0.0 0.0 2.630917932860768 0.0 88 0.0 0.0 0.0 2.8300814894560853 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5360 0.0 57.180523 1 TACCTGG 2355 0.0 48.69654 2 ATAACGC 295 0.0 47.796753 3 GTATCAA 10985 0.0 45.530968 1 TATAACG 280 0.0 43.64299 2 GTACCTG 2775 0.0 42.348923 1 TAACGCA 380 0.0 37.10457 4 ACCTGGG 3155 0.0 36.348763 3 ATCAACG 14165 0.0 35.304024 3 TCAACGC 14240 0.0 35.282345 4 TATCAAC 14300 0.0 34.937866 2 CAACGCA 14420 0.0 34.841164 5 AACGCAG 14940 0.0 33.754322 6 ACGCAGA 16710 0.0 30.12266 7 CGCAGAG 16750 0.0 29.994604 8 ACAACGC 315 0.0 29.841358 3 TACAACG 285 0.0 29.6843 2 TCTGTCG 710 0.0 29.12558 8 GCAGAGT 17860 0.0 27.814661 9 GTACATG 8105 0.0 23.953136 1 >>END_MODULE